MD03G1266900.v1.1

protein trichome birefringence-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr03
OG0015547
Physical Location & Seq
Reverse (-)
35182345 .. 35184796
2452 bp
Loading structure...
UTR
Exon/CDS
Intron
MD03G1266900.v1.1.491

Sequence Viewer

Length: 852 bp
ATGGCGAATAATAATCCCGCGTCTCCACATCAAACAGAGGAAAGCACAATGACCCACTTGGAGTTCTTCAAGAAACTCAAACGCTCCAACCCTCTTGAACCTTCTCTGGGGATTCTTGGGTTCTTCTTGGTCGCTCTTCTCTTCGTTAGCTGCTTCTCCTACTTGGATTACCGTACAGTTTACAGTCGGATTCTGGGGTTGCCGTCAAAAGGGAATGGACTGCTGGGATTTCTTGAGGAAGGTGGTAACGGCTGTGACGTGTTTGATGGGAATTGGGTTTGGGATGAGAGCTATCCGTTGTACGAGTCTCAGAATTGTAGCCTTTTAGATGAAGGGTTTAGATGTTCTGAGAATGGCCGGCCTGATAATTTCTACACCAAGTGGCGTTGGCAGCCCAAAGGTTGCACCTTGCCCAGATTTGATGCAGGGAAGATGTTGGAGAAGCTTCGGAACCGGCGTGTTGTATTTGTTGGTGACTCAATTGGAAGGAACCAATGGGAGTCTCTTCTCTGTATGCTCTCCTCTGCAGTACCTAACAAGACTTCAATCTATGAGGTGAATGGGAGTCCAATCACAAAGCATTCGGGGTTCTTGGTATTCAAGTTTGAGGACTTCAACTGCACTGTGGAATACTACAGAGCACCATTTCTAGTCTTTCAGGGCCACCCTCCTGCTGGTGCTCCAGAAAACGTGAAGATAACCTTAAGGGTGGATCAACTGGAAAGGACTTCCAGGCAGTGGAGAGATGCAGATGTGCTGATTCTCAACGCGGGGCATTGGTGGAGTTATGAGAAGACCATACGAGAGAGAGGAGGTGAAGATGAATATGACTGTCGAAACAGCATTTCGTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
PC-Esterase PMR5N

Protein Analysis

284

Amino Acids

32.48

Weight (kDa)

6.02

Isoelectric Point (pI)

44.7

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PMR5N PF14416 84 - 135 1.4e-22 PMR5 N terminal Domain
PC-Esterase PF13839 137 - 270 5.3e-46 GDSL/SGNH-like Acyl-Esterase family found in Pmr5 and Cas1p
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0015547)

Species Orthologous Gene IDs
fragaria_vesca FvH4_2g23720
malus_domestica MD03G1266900.v1.1 MD11G1287300.v1.1
prunus_persica Prupe.8G242600_v2.0.a1
pyrus_communis pycom03g21320 pycom11g25470
rosa_chinensis RchiOBHm_Chr6g0291221
rosa_laevigata RLG00000012132
rosa_multiflora Rmu_sc0013203.1_g000020
rosa_roxburghii Rroxscaffold_7G00176240
rosa_rugosa Rorug06G0218500
rosa_samantha Rh6AG329300 Rh6BG336500 Rh6CG342800 Rh6DG329800
rosa_wichuraiana Rw6G028630

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB7I CCANNNNNTGG 1 cut(s) 738
AccII CGCG 2 cut(s) 20, 770
AciI CCGC 2 cut(s) 18, 770
AclWI GGATC 1 cut(s) 720
AcoI YGGCCR 1 cut(s) 355
AdeI CACNNNGTG 1 cut(s) 381
AfaI GTAC 3 cut(s) 175, 302, 531
AfiI CCNNNNNNNGG 4 cut(s) 107, 209, 674, 738
AflII CTTAAG 1 cut(s) 703
AflIII ACRYGT 1 cut(s) 258
AgsI TTSAA 5 cut(s) 70, 98, 546, 601, 616
AjiI CACGTC 1 cut(s) 259
AjnI CCWGG 1 cut(s) 731
AluBI AGCT 3 cut(s) 150, 291, 445
AluI AGCT 3 cut(s) 150, 291, 445
Alw21I GWGCWC 2 cut(s) 643, 682
Alw26I GTCTC 3 cut(s) 27, 312, 507
AlwI GGATC 1 cut(s) 720
AoxI GGCC 3 cut(s) 355, 359, 661
ApeKI GCWGC 2 cut(s) 150, 391
AspS9I GGNCC 1 cut(s) 661
AsuHPI GGTGA 3 cut(s) 485, 568, 827
BbsI GAAGAC 1 cut(s) 800
Bbv12I GWGCWC 2 cut(s) 643, 682
BbvI GCAGC 2 cut(s) 137, 403
BccI CCATC 1 cut(s) 260
BceAI ACGGC 2 cut(s) 187, 265
BciT130I CCWGG 1 cut(s) 733
BcoDI GTCTC 3 cut(s) 27, 312, 507
BfaI CTAG 1 cut(s) 650
BfmI CTRYAG 2 cut(s) 525, 634
BfrI CTTAAG 1 cut(s) 703
BisI GCNGC 2 cut(s) 151, 392
BlsI GCNGC 2 cut(s) 152, 393
Bme1390I CCNGG 1 cut(s) 733
BmgBI CACGTC 1 cut(s) 259
BmgT120I GGNCC 1 cut(s) 661
BmiI GGNNCC 2 cut(s) 452, 491
BmrFI CCNGG 1 cut(s) 733
BmsI GCATC 2 cut(s) 412, 736
BpiI GAAGAC 1 cut(s) 800
BpmI CTGGAG 1 cut(s) 666
BpuEI CTTGAG 1 cut(s) 254
Bsc4I CCNNNNNNNGG 4 cut(s) 107, 209, 674, 738
Bse118I RCCGGY 2 cut(s) 357, 453
Bse1I ACTGG 1 cut(s) 723
BseBI CCWGG 1 cut(s) 733
BseGI GGATG 1 cut(s) 289
BseLI CCNNNNNNNGG 4 cut(s) 107, 209, 674, 738
BseMII CTCAG 2 cut(s) 323, 339
BseNI ACTGG 1 cut(s) 723
BseRI GAGGAG 2 cut(s) 511, 825
BseXI GCAGC 2 cut(s) 137, 403
BseYI CCCAGC 1 cut(s) 223
BsgI GTGCAG 1 cut(s) 604
Bsh1236I CGCG 2 cut(s) 20, 770
BshFI GGCC 3 cut(s) 357, 361, 663
BsiHKAI GWGCWC 2 cut(s) 643, 682
BsiSI CCGG 2 cut(s) 358, 454
BslI CCNNNNNNNGG 4 cut(s) 107, 209, 674, 738
BsmAI GTCTC 3 cut(s) 27, 312, 507
BsmBI CGTCTC 1 cut(s) 27
BsmI GAATGC 1 cut(s) 580
BsnI GGCC 3 cut(s) 357, 361, 663
Bsp1286I GDGCHC 2 cut(s) 643, 682
Bsp143I GATC 1 cut(s) 712
BspACI CCGC 2 cut(s) 18, 770
BspANI GGCC 3 cut(s) 357, 361, 663
BspCNI CTCAG 2 cut(s) 322, 340
BspFNI CGCG 2 cut(s) 20, 770
BspLI GGNNCC 2 cut(s) 452, 491
BspMAI CTGCAG 1 cut(s) 529
BspPI GGATC 1 cut(s) 720
BspQI GCTCTTC 1 cut(s) 141
BspTI CTTAAG 1 cut(s) 703
BsrFI RCCGGY 2 cut(s) 357, 453
BsrI ACTGG 1 cut(s) 723
BssAI RCCGGY 2 cut(s) 357, 453
BssMI GATC 1 cut(s) 712
Bst2UI CCWGG 1 cut(s) 733
Bst4CI ACNGT 5 cut(s) 173, 178, 185, 625, 833
Bst6I CTCTTC 3 cut(s) 141, 146, 510
BstAFI CTTAAG 1 cut(s) 703
BstC8I GCNNGC 1 cut(s) 359
BstDEI CTNAG 2 cut(s) 309, 348
BstF5I GGATG 1 cut(s) 289
BstFNI CGCG 2 cut(s) 20, 770
BstKTI GATC 1 cut(s) 715
BstMAI GTCTC 3 cut(s) 27, 312, 507
BstMBI GATC 1 cut(s) 712
BstMWI GCNNNNNNNGC 1 cut(s) 391
BstNI CCWGG 1 cut(s) 733
BstSCI CCNGG 1 cut(s) 731
BstSFI CTRYAG 2 cut(s) 525, 634
BstUI CGCG 2 cut(s) 20, 770
BstV1I GCAGC 2 cut(s) 137, 403
BstV2I GAAGAC 1 cut(s) 800
BsuRI GGCC 3 cut(s) 357, 361, 663
BtrI CACGTC 1 cut(s) 259
BtsCI GGATG 1 cut(s) 289
BtsI GCAGTG 1 cut(s) 743
BtsIMutI CAGTG 2 cut(s) 621, 743
Cac8I GCNNGC 1 cut(s) 359
Cfr10I RCCGGY 2 cut(s) 357, 453
Cfr13I GGNCC 1 cut(s) 661
CseI GACGC 1 cut(s) 9
Csp6I GTAC 3 cut(s) 174, 301, 530
CviJI RGCY 9 cut(s) 150, 252, 291, 321, 357, 361, 394, 445, 663
CviKI_1 RGCY 9 cut(s) 150, 252, 291, 321, 357, 361, 394, 445, 663
CviQI GTAC 3 cut(s) 174, 301, 530
DdeI CTNAG 2 cut(s) 309, 348
DpnI GATC 1 cut(s) 714
DpnII GATC 1 cut(s) 712
DraIII CACNNNGTG 1 cut(s) 381
EaeI YGGCCR 1 cut(s) 355
Eam1104I CTCTTC 3 cut(s) 141, 146, 510
EarI CTCTTC 3 cut(s) 141, 146, 510
EcoRII CCWGG 1 cut(s) 731
Esp3I CGTCTC 1 cut(s) 27
FaiI YATR 5 cut(s) 515, 552, 789, 800, 828
FalI AAGNNNNNCTT 2 cut(s) 686, 718
FauI CCCGC 2 cut(s) 25, 763
Fnu4HI GCNGC 2 cut(s) 151, 392
FokI GGATG 1 cut(s) 296
FseI GGCCGGCC 1 cut(s) 361
Fsp4HI GCNGC 2 cut(s) 151, 392
FspBI CTAG 1 cut(s) 650
GluI GCNGC 2 cut(s) 151, 392
GsaI CCCAGC 1 cut(s) 227
GsuI CTGGAG 1 cut(s) 666
HaeIII GGCC 3 cut(s) 357, 361, 663
HapII CCGG 2 cut(s) 358, 454
HgaI GACGC 1 cut(s) 9
HindIII AAGCTT 1 cut(s) 443
HinfI GANTC 7 cut(s) 112, 190, 305, 476, 500, 565, 760
HpaII CCGG 2 cut(s) 358, 454
HphI GGTGA 3 cut(s) 485, 568, 827
Hpy166II GTNNAC 1 cut(s) 181
Hpy188I TCNGA 4 cut(s) 189, 312, 349, 450
Hpy188III TCNNGA 4 cut(s) 70, 95, 233, 683
Hpy8I GTNNAC 1 cut(s) 181
HpyAV CCTTC 4 cut(s) 111, 233, 326, 480
HpyCH4III ACNGT 5 cut(s) 173, 178, 185, 625, 833
HpyCH4IV ACGT 2 cut(s) 258, 690
HpyCH4V TGCA 5 cut(s) 405, 425, 527, 621, 749
HpyF10VI GCNNNNNNNGC 1 cut(s) 391
HpyF3I CTNAG 2 cut(s) 309, 348
HpySE526I ACGT 2 cut(s) 258, 690
KroI GCCGGC 1 cut(s) 357
KroNI GCCGGC 1 cut(s) 359
Kzo9I GATC 1 cut(s) 712
LguI GCTCTTC 1 cut(s) 141
LmnI GCTCC 2 cut(s) 89, 685
Lsp1109I GCAGC 2 cut(s) 137, 403
LweI GCATC 2 cut(s) 412, 736
MaeI CTAG 1 cut(s) 650
MaeII ACGT 2 cut(s) 258, 690
MaeIII GTNAC 3 cut(s) 245, 254, 473
MalI GATC 1 cut(s) 714
MboI GATC 1 cut(s) 712
MboII GAAGA 9 cut(s) 58, 115, 128, 133, 442, 497, 706, 805, 830
MfeI CAATTG 1 cut(s) 480
MhlI GDGCHC 2 cut(s) 643, 682
MluCI AATT 4 cut(s) 271, 313, 367, 480
MlyI GAGTC 4 cut(s) 314, 470, 509, 574
MmeI TCCRAC 3 cut(s) 111, 167, 417
MnlI CCTC 9 cut(s) 31, 102, 229, 532, 547, 601, 678, 803, 806
MroNI GCCGGC 1 cut(s) 357
MseI TTAA 1 cut(s) 704
MspCI CTTAAG 1 cut(s) 703
MspI CCGG 2 cut(s) 358, 454
MspR9I CCNGG 1 cut(s) 733
MunI CAATTG 1 cut(s) 480
Mva1269I GAATGC 1 cut(s) 580
MvaI CCWGG 1 cut(s) 733
MvnI CGCG 2 cut(s) 20, 770
MwoI GCNNNNNNNGC 1 cut(s) 391
NaeI GCCGGC 1 cut(s) 359
NdeII GATC 1 cut(s) 712
NgoMIV GCCGGC 1 cut(s) 357
NlaIV GGNNCC 2 cut(s) 452, 491
NmuCI GTSAC 2 cut(s) 254, 473
PciSI GCTCTTC 1 cut(s) 141
PcsI WCGNNNNNNNCGW 2 cut(s) 255, 454
PctI GAATGC 1 cut(s) 580
PdiI GCCGGC 1 cut(s) 359
PfeI GAWTC 3 cut(s) 112, 190, 760
PflMI CCANNNNNTGG 1 cut(s) 738
PkrI GCNGC 2 cut(s) 152, 393
PleI GAGTC 4 cut(s) 313, 470, 508, 573
PpsI GAGTC 4 cut(s) 313, 470, 508, 573
Psp6I CCWGG 1 cut(s) 731
PspFI CCCAGC 1 cut(s) 223
PspGI CCWGG 1 cut(s) 731
PspN4I GGNNCC 2 cut(s) 452, 491
PspPI GGNCC 1 cut(s) 661
PstI CTGCAG 1 cut(s) 529
RigI GGCCGGCC 1 cut(s) 361
RsaI GTAC 3 cut(s) 175, 302, 531
RsaNI GTAC 3 cut(s) 174, 301, 530
SapI GCTCTTC 1 cut(s) 141
SaqAI TTAA 1 cut(s) 704
SatI GCNGC 2 cut(s) 151, 392
Sau3AI GATC 1 cut(s) 712
Sau96I GGNCC 1 cut(s) 661
SchI GAGTC 4 cut(s) 314, 470, 509, 574
ScrFI CCNGG 1 cut(s) 733
SduI GDGCHC 2 cut(s) 643, 682
SfaNI GCATC 2 cut(s) 412, 736
SfcI CTRYAG 2 cut(s) 525, 634
SmlI CTYRAG 2 cut(s) 233, 703
SmoI CTYRAG 2 cut(s) 233, 703
Sse9I AATT 4 cut(s) 271, 313, 367, 480
SsiI CCGC 2 cut(s) 18, 770
SspMI CTAG 1 cut(s) 650
StyD4I CCNGG 1 cut(s) 731
TaaI ACNGT 5 cut(s) 173, 178, 185, 625, 833
TaiI ACGT 2 cut(s) 261, 693
TaqI TCGA 1 cut(s) 835
TasI AATT 4 cut(s) 271, 313, 367, 480
TfiI GAWTC 3 cut(s) 112, 190, 760
Tru1I TTAA 1 cut(s) 704
Tru9I TTAA 1 cut(s) 704
TscAI CASTG 2 cut(s) 628, 743
TseFI GTSAC 2 cut(s) 254, 473
TseI GCWGC 2 cut(s) 150, 391
Tsp45I GTSAC 2 cut(s) 254, 473
TspDTI ATGAA 2 cut(s) 345, 837
TspGWI ACGGA 1 cut(s) 285
TspRI CASTG 2 cut(s) 628, 743
Van91I CCANNNNNTGG 1 cut(s) 738
Vha464I CTTAAG 1 cut(s) 703
XcmI CCANNNNNNNNNTGG 1 cut(s) 671
XspI CTAG 1 cut(s) 650
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.