Rh6BG336500

protein trichome birefringence-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr6B
OG0015547
Physical Location & Seq
Reverse (-)
56991248 .. 56995987
4740 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh6BG336500.1

Sequence Viewer

Length: 906 bp
ATGATGTTGGAAAAGCTTCGAAACCGACGTCTTGTATTTGCTGGTGACTCGATTGGAAGGAACCAGTGGGAGTCTCTTCTCTGCATGCTCTCTTCTGTAATTCCTAACAAGACTTCAATCTATGAGGTGAATGGGAGGCCAATCACGAAGCACTCAGGGTTTTTGGTCTTTAAGTTTGAGGACTTCAACTGCACAGTGGAATACTACAGGTCACCTTTTCTAGTCTTTCAGGGCCATCCCCCGGCTGGCGCCCCTGAGGAGGTGAAGTTGGCTTTAAAAGTGGATAAAATGGACTGGATTTCCCGCCACTGGAGGGATGCAGATGTGCTGGTTTTGAATGCTGGGCACTGGTGGAATTATGGGAAGACCATAAGAGAGGGTTGCTACTTTCAAGAAGGGGAGGAGATAAAGATGAATATGACTGTTAAAACTGCATACCAGAGATCAATGGAAACTTTGCTTGATTGGATTGATAGTGAAGTCAACATAAGCAAGACTTATGTACTTTTTCGAACTTATGCTCCTGTGCATTTCAGTGGTGGTGATTGGAATGCTGGCGGTGGTTGTCACTTGGAAACTCTACCTGGATTAGGCCCATTGCCTGTTTTATCCGACAGTGTTTACAAGACTATCTTTGATGTGTTATCTGAGCGGTCAAATGAGTCGCATGTAAGAAAATTCGATTTTCTGAATGTAACAAACATGTCTCTATGGAGAAAAGATGGCCACGCATCTCTGTACTATCTAGGGCCTAATGGAGCAGCCCCTATCAACCATCAAGACTGCAGCCACTGGTGCCTACCTGGTGTCCCTGATGCATGGAACGAACTTCTGTATGCCCTTTTCCTAAAACAGGAATCCATTCGCTCAGGAAATTCAACCAAATCTTCTCAGCTTCCGTTGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
PC-Esterase PMR5N

Protein Analysis

301

Amino Acids

34.38

Weight (kDa)

6.55

Isoelectric Point (pI)

50.79

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PC-Esterase PF13839 2 - 280 1.3e-92 GDSL/SGNH-like Acyl-Esterase family found in Pmr5 and Cas1p
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0015547)

Species Orthologous Gene IDs
fragaria_vesca FvH4_2g23720
malus_domestica MD03G1266900.v1.1 MD11G1287300.v1.1
prunus_persica Prupe.8G242600_v2.0.a1
pyrus_communis pycom03g21320 pycom11g25470
rosa_chinensis RchiOBHm_Chr6g0291221
rosa_laevigata RLG00000012132
rosa_multiflora Rmu_sc0013203.1_g000020
rosa_roxburghii Rroxscaffold_7G00176240
rosa_rugosa Rorug06G0218500
rosa_samantha Rh6AG329300 Rh6BG336500 Rh6CG342800 Rh6DG329800
rosa_wichuraiana Rw6G028630

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 31
AccB1I GGYRCC 2 cut(s) 248, 795
AccBSI CCGCTC 1 cut(s) 652
AciI CCGC 3 cut(s) 304, 558, 652
AcoI YGGCCR 1 cut(s) 724
AcsI RAATTY 2 cut(s) 677, 874
AcyI GRCGYC 2 cut(s) 28, 249
AfaI GTAC 2 cut(s) 504, 740
AfiI CCNNNNNNNGG 7 cut(s) 241, 245, 259, 309, 313, 590, 853
AflIII ACRYGT 1 cut(s) 702
AgsI TTSAA 5 cut(s) 117, 187, 337, 392, 879
AjnI CCWGG 2 cut(s) 583, 802
AloI GAACNNNNNNTCC 2 cut(s) 505, 537
AluBI AGCT 2 cut(s) 16, 895
AluI AGCT 2 cut(s) 16, 895
Alw26I GTCTC 2 cut(s) 78, 711
AlwNI CAGNNNCTG 1 cut(s) 792
AoxI GGCC 5 cut(s) 137, 232, 592, 724, 749
ApeKI GCWGC 2 cut(s) 761, 786
ApoI RAATTY 2 cut(s) 677, 874
ArsI GACNNNNNNTTYG 2 cut(s) 13, 45
Asp700I GAANNNNTTC 2 cut(s) 15, 861
AspLEI GCGC 1 cut(s) 251
AspS9I GGNCC 3 cut(s) 232, 593, 749
AsuC2I CCSGG 1 cut(s) 242
AsuHPI GGTGA 5 cut(s) 56, 139, 204, 274, 554
AsuII TTCGAA 2 cut(s) 19, 511
AxyI CCTNAGG 1 cut(s) 255
BaeGI GKGCMC 1 cut(s) 348
BalI TGGCCA 1 cut(s) 726
BanI GGYRCC 2 cut(s) 248, 795
BbsI GAAGAC 1 cut(s) 371
BbvI GCAGC 2 cut(s) 773, 798
BccI CCATC 3 cut(s) 243, 716, 783
BciT130I CCWGG 2 cut(s) 585, 804
BcnI CCSGG 1 cut(s) 242
BcoDI GTCTC 2 cut(s) 78, 711
BfaI CTAG 2 cut(s) 221, 746
BfmI CTRYAG 2 cut(s) 205, 784
BfoI RGCGCY 1 cut(s) 252
BisI GCNGC 2 cut(s) 762, 787
BlsI GCNGC 2 cut(s) 763, 788
Bme1390I CCNGG 3 cut(s) 242, 585, 804
BmgT120I GGNCC 3 cut(s) 232, 593, 749
BmiI GGNNCC 3 cut(s) 62, 250, 797
BmrFI CCNGG 3 cut(s) 242, 585, 804
BmsI GCATC 3 cut(s) 307, 740, 805
BpiI GAAGAC 1 cut(s) 371
BpmI CTGGAG 1 cut(s) 331
Bpu10I CCTNAGC 1 cut(s) 868
Bpu14I TTCGAA 2 cut(s) 19, 511
BpuMI CCSGG 1 cut(s) 242
BsaHI GRCGYC 2 cut(s) 28, 249
BsaJI CCNNGG 1 cut(s) 240
BsaXI ACNNNNNCTCC 4 cut(s) 251, 281, 505, 535
Bsc4I CCNNNNNNNGG 7 cut(s) 241, 245, 259, 309, 313, 590, 853
Bse1I ACTGG 5 cut(s) 64, 299, 314, 353, 797
Bse21I CCTNAGG 1 cut(s) 255
Bse3DI GCAATG 1 cut(s) 596
BseBI CCWGG 2 cut(s) 585, 804
BseDI CCNNGG 1 cut(s) 240
BseGI GGATG 2 cut(s) 235, 322
BseLI CCNNNNNNNGG 7 cut(s) 241, 245, 259, 309, 313, 590, 853
BseMI GCAATG 1 cut(s) 596
BseMII CTCAG 5 cut(s) 168, 246, 639, 882, 905
BseNI ACTGG 5 cut(s) 64, 299, 314, 353, 797
BseRI GAGGAG 2 cut(s) 272, 416
BseSI GKGCMC 1 cut(s) 348
BseXI GCAGC 2 cut(s) 773, 798
BseYI CCCAGC 1 cut(s) 341
BsgI GTGCAG 1 cut(s) 175
BshFI GGCC 5 cut(s) 139, 234, 594, 726, 751
BshNI GGYRCC 2 cut(s) 248, 795
BsiSI CCGG 1 cut(s) 242
BslFI GGGAC 1 cut(s) 794
BslI CCNNNNNNNGG 7 cut(s) 241, 245, 259, 309, 313, 590, 853
BsmAI GTCTC 2 cut(s) 78, 711
BsmFI GGGAC 1 cut(s) 794
BsmI GAATGC 2 cut(s) 343, 556
BsnI GGCC 5 cut(s) 139, 234, 594, 726, 751
Bsp119I TTCGAA 2 cut(s) 19, 511
Bsp1286I GDGCHC 1 cut(s) 348
Bsp143I GATC 1 cut(s) 443
BspACI CCGC 3 cut(s) 304, 558, 652
BspANI GGCC 5 cut(s) 139, 234, 594, 726, 751
BspCNI CTCAG 5 cut(s) 167, 247, 640, 881, 904
BspLI GGNNCC 3 cut(s) 62, 250, 797
BspMAI CTGCAG 1 cut(s) 788
BspT104I TTCGAA 2 cut(s) 19, 511
BspT107I GGYRCC 2 cut(s) 248, 795
BsrBI CCGCTC 1 cut(s) 652
BsrDI GCAATG 1 cut(s) 596
BsrI ACTGG 5 cut(s) 64, 299, 314, 353, 797
BssECI CCNNGG 1 cut(s) 240
BssMI GATC 1 cut(s) 443
BssNI GRCGYC 2 cut(s) 28, 249
Bst2UI CCWGG 2 cut(s) 585, 804
Bst4CI ACNGT 3 cut(s) 196, 424, 617
Bst6I CTCTTC 2 cut(s) 81, 97
BstACI GRCGYC 2 cut(s) 28, 249
BstBI TTCGAA 2 cut(s) 19, 511
BstC8I GCNNGC 3 cut(s) 86, 247, 556
BstDEI CTNAG 5 cut(s) 154, 255, 648, 868, 891
BstEII GGTNACC 1 cut(s) 210
BstENI CCTNNNNNAGG 2 cut(s) 588, 851
BstF5I GGATG 2 cut(s) 235, 322
BstH2I RGCGCY 1 cut(s) 252
BstHHI GCGC 1 cut(s) 251
BstKTI GATC 1 cut(s) 446
BstMAI GTCTC 2 cut(s) 78, 711
BstMBI GATC 1 cut(s) 443
BstMWI GCNNNNNNNGC 1 cut(s) 795
BstNI CCWGG 2 cut(s) 585, 804
BstNSI RCATGY 3 cut(s) 88, 671, 706
BstPI GGTNACC 1 cut(s) 210
BstSCI CCNGG 3 cut(s) 240, 583, 802
BstSFI CTRYAG 2 cut(s) 205, 784
BstSLI GKGCMC 1 cut(s) 348
BstV1I GCAGC 2 cut(s) 773, 798
BstV2I GAAGAC 1 cut(s) 371
Bsu36I CCTNAGG 1 cut(s) 255
BsuRI GGCC 5 cut(s) 139, 234, 594, 726, 751
BtsCI GGATG 2 cut(s) 235, 322
BtsIMutI CAGTG 7 cut(s) 71, 201, 307, 346, 541, 622, 790
Cac8I GCNNGC 3 cut(s) 86, 247, 556
CaiI CAGNNNCTG 1 cut(s) 792
CfoI GCGC 1 cut(s) 251
Cfr13I GGNCC 3 cut(s) 232, 593, 749
CsiI ACCWGGT 1 cut(s) 802
Csp6I GTAC 2 cut(s) 503, 739
CviAII CATG 4 cut(s) 85, 668, 703, 819
CviQI GTAC 2 cut(s) 503, 739
DdeI CTNAG 5 cut(s) 154, 255, 648, 868, 891
DinI GGCGCC 1 cut(s) 250
DpnI GATC 1 cut(s) 445
DpnII GATC 1 cut(s) 443
DraI TTTAAA 1 cut(s) 276
EaeI YGGCCR 1 cut(s) 724
Eam1104I CTCTTC 2 cut(s) 81, 97
EarI CTCTTC 2 cut(s) 81, 97
Eco81I CCTNAGG 1 cut(s) 255
Eco91I GGTNACC 1 cut(s) 210
EcoNI CCTNNNNNAGG 2 cut(s) 588, 851
EcoO109I RGGNCCY 1 cut(s) 749
EcoO65I GGTNACC 1 cut(s) 210
EcoRII CCWGG 2 cut(s) 583, 802
EcoT22I ATGCAT 1 cut(s) 820
EgeI GGCGCC 1 cut(s) 250
EheI GGCGCC 1 cut(s) 250
FaeI CATG 4 cut(s) 88, 671, 706, 822
FalI AAGNNNNNCTT 4 cut(s) 481, 513, 617, 649
FaqI GGGAC 1 cut(s) 794
FatI CATG 4 cut(s) 84, 667, 702, 818
FauI CCCGC 1 cut(s) 311
Fnu4HI GCNGC 2 cut(s) 762, 787
FokI GGATG 2 cut(s) 222, 329
Fsp4HI GCNGC 2 cut(s) 762, 787
FspBI CTAG 2 cut(s) 221, 746
GlaI GCGC 1 cut(s) 250
GluI GCNGC 2 cut(s) 762, 787
GsaI CCCAGC 1 cut(s) 345
GsuI CTGGAG 1 cut(s) 331
HaeII RGCGCY 1 cut(s) 252
HaeIII GGCC 5 cut(s) 139, 234, 594, 726, 751
HapII CCGG 1 cut(s) 242
HhaI GCGC 1 cut(s) 251
Hin1I GRCGYC 2 cut(s) 28, 249
Hin1II CATG 4 cut(s) 88, 671, 706, 822
Hin6I GCGC 1 cut(s) 249
HinP1I GCGC 1 cut(s) 249
HincII GTYRAC 1 cut(s) 484
HindII GTYRAC 1 cut(s) 484
HindIII AAGCTT 1 cut(s) 14
HinfI GANTC 4 cut(s) 47, 71, 662, 857
HpaII CCGG 1 cut(s) 242
HphI GGTGA 5 cut(s) 56, 139, 204, 274, 554
Hpy166II GTNNAC 2 cut(s) 484, 622
Hpy188I TCNGA 3 cut(s) 613, 649, 690
Hpy188III TCNNGA 4 cut(s) 145, 392, 779, 870
Hpy8I GTNNAC 2 cut(s) 484, 622
Hpy99I CGWCG 1 cut(s) 30
HpyAV CCTTC 2 cut(s) 51, 389
HpyCH4III ACNGT 3 cut(s) 196, 424, 617
HpyCH4IV ACGT 1 cut(s) 28
HpyCH4V TGCA 7 cut(s) 84, 192, 320, 434, 529, 786, 818
HpyF10VI GCNNNNNNNGC 1 cut(s) 795
HpyF3I CTNAG 5 cut(s) 154, 255, 648, 868, 891
HpySE526I ACGT 1 cut(s) 28
Hsp92I GRCGYC 2 cut(s) 28, 249
Hsp92II CATG 4 cut(s) 88, 671, 706, 822
HspAI GCGC 1 cut(s) 249
KasI GGCGCC 1 cut(s) 248
Kzo9I GATC 1 cut(s) 443
LmnI GCTCC 2 cut(s) 526, 758
Lsp1109I GCAGC 2 cut(s) 773, 798
LweI GCATC 3 cut(s) 307, 740, 805
MabI ACCWGGT 1 cut(s) 802
MaeI CTAG 2 cut(s) 221, 746
MaeII ACGT 1 cut(s) 28
MaeIII GTNAC 4 cut(s) 44, 210, 566, 694
MalI GATC 1 cut(s) 445
MbiI CCGCTC 1 cut(s) 652
MboI GATC 1 cut(s) 443
MboII GAAGA 4 cut(s) 68, 84, 376, 879
MhlI GDGCHC 1 cut(s) 348
MlsI TGGCCA 1 cut(s) 726
MluCI AATT 4 cut(s) 99, 355, 677, 874
MluNI TGGCCA 1 cut(s) 726
Mly113I GGCGCC 1 cut(s) 249
MlyI GAGTC 3 cut(s) 41, 80, 671
MmeI TCCRAC 1 cut(s) 636
MnlI CCTC 8 cut(s) 118, 129, 172, 250, 253, 306, 370, 394
Mox20I TGGCCA 1 cut(s) 726
Mph1103I ATGCAT 1 cut(s) 820
MroXI GAANNNNTTC 2 cut(s) 15, 861
MscI TGGCCA 1 cut(s) 726
MseI TTAA 3 cut(s) 171, 275, 426
MslI CAYNNNNRTG 1 cut(s) 534
Msp20I TGGCCA 1 cut(s) 726
MspI CCGG 1 cut(s) 242
MspR9I CCNGG 3 cut(s) 242, 585, 804
Mva1269I GAATGC 2 cut(s) 343, 556
MvaI CCWGG 2 cut(s) 585, 804
MwoI GCNNNNNNNGC 1 cut(s) 795
NarI GGCGCC 1 cut(s) 249
NciI CCSGG 1 cut(s) 242
NdeII GATC 1 cut(s) 443
NlaIII CATG 4 cut(s) 88, 671, 706, 822
NlaIV GGNNCC 3 cut(s) 62, 250, 797
NmuCI GTSAC 3 cut(s) 44, 210, 566
NsiI ATGCAT 1 cut(s) 820
NspI RCATGY 3 cut(s) 88, 671, 706
NspV TTCGAA 2 cut(s) 19, 511
PaeI GCATGC 1 cut(s) 88
PciI ACATGT 1 cut(s) 702
PcsI WCGNNNNNNNCGW 1 cut(s) 25
PctI GAATGC 2 cut(s) 343, 556
PdmI GAANNNNTTC 2 cut(s) 15, 861
PfeI GAWTC 1 cut(s) 857
PkrI GCNGC 2 cut(s) 763, 788
PleI GAGTC 3 cut(s) 41, 79, 670
PluTI GGCGCC 1 cut(s) 252
PpsI GAGTC 3 cut(s) 41, 79, 670
PscI ACATGT 1 cut(s) 702
Psp6I CCWGG 2 cut(s) 583, 802
PspEI GGTNACC 1 cut(s) 210
PspFI CCCAGC 1 cut(s) 341
PspGI CCWGG 2 cut(s) 583, 802
PspN4I GGNNCC 3 cut(s) 62, 250, 797
PspPI GGNCC 3 cut(s) 232, 593, 749
PstI CTGCAG 1 cut(s) 788
PstNI CAGNNNCTG 1 cut(s) 792
RsaI GTAC 2 cut(s) 504, 740
RsaNI GTAC 2 cut(s) 503, 739
RseI CAYNNNNRTG 1 cut(s) 534
SaqAI TTAA 3 cut(s) 171, 275, 426
SatI GCNGC 2 cut(s) 762, 787
Sau3AI GATC 1 cut(s) 443
Sau96I GGNCC 3 cut(s) 232, 593, 749
SchI GAGTC 3 cut(s) 41, 80, 671
ScrFI CCNGG 3 cut(s) 242, 585, 804
SduI GDGCHC 1 cut(s) 348
SetI ASST 9 cut(s) 18, 31, 129, 212, 217, 264, 586, 805, 897
SexAI ACCWGGT 1 cut(s) 802
SfaNI GCATC 3 cut(s) 307, 740, 805
SfcI CTRYAG 2 cut(s) 205, 784
SfoI GGCGCC 1 cut(s) 250
SfuI TTCGAA 2 cut(s) 19, 511
SmiMI CAYNNNNRTG 1 cut(s) 534
SphI GCATGC 1 cut(s) 88
Sse9I AATT 4 cut(s) 99, 355, 677, 874
SsiI CCGC 3 cut(s) 304, 558, 652
SspDI GGCGCC 1 cut(s) 248
SspMI CTAG 2 cut(s) 221, 746
StyD4I CCNGG 3 cut(s) 240, 583, 802
TaaI ACNGT 3 cut(s) 196, 424, 617
TaiI ACGT 1 cut(s) 31
TaqI TCGA 4 cut(s) 19, 50, 511, 681
TasI AATT 4 cut(s) 99, 355, 677, 874
TatI WGTACW 2 cut(s) 502, 738
TfiI GAWTC 1 cut(s) 857
Tru1I TTAA 3 cut(s) 171, 275, 426
Tru9I TTAA 3 cut(s) 171, 275, 426
TscAI CASTG 7 cut(s) 71, 201, 314, 353, 541, 622, 797
TseFI GTSAC 3 cut(s) 44, 210, 566
TseI GCWGC 2 cut(s) 761, 786
Tsp45I GTSAC 3 cut(s) 44, 210, 566
TspDTI ATGAA 1 cut(s) 428
TspGWI ACGGA 1 cut(s) 888
TspRI CASTG 7 cut(s) 71, 201, 314, 353, 541, 622, 797
XagI CCTNNNNNAGG 2 cut(s) 588, 851
XapI RAATTY 2 cut(s) 677, 874
XceI RCATGY 3 cut(s) 88, 671, 706
XcmI CCANNNNNNNNNTGG 1 cut(s) 242
XmnI GAANNNNTTC 2 cut(s) 15, 861
XspI CTAG 2 cut(s) 221, 746
ZraI GACGTC 1 cut(s) 29
Zsp2I ATGCAT 1 cut(s) 820
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.