MD03G1271900.v1.1

methyl-CpG-binding domain-containing protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr03
OG0013011
Physical Location & Seq
Reverse (-)
35511992 .. 35514773
2782 bp
Loading structure...
UTR
Exon/CDS
Intron
MD03G1271900.v1.1.491

Sequence Viewer

Length: 570 bp
ATGGACGGCCAAACCCCCAAAACGTCGTCGTCCAAGAAACCAAATGTAGCGAAAAGTTCGATTGATGTATATGCTGCGCAGTGTGACCAATGCAAGAAATGGAGAGTGATTCATTCTGAAGAAGAGTACGAGGAAATCAGGAGCAAAATCAGTACGGAACCCTTTTATTGCAACAAAAAATCTGGTGTGACTTGTGACGATGCTGCCGATATTGAGTACAGTGCTAGCAGGACCTGGGTCATTGATAAGCCTGATCTTCCGAAGACCCCGGAAGGTTTCAGGAGACATTTGGTGGCTAGAAAAGATTATTCCAAACTGGATACGTATTATATTACACCCAAGGGAAAAAGACTGAGATCCAGAAATGAGATGGCAGCATTTTTAAAACAAAACCGTAAGTATGAAGGCTTATCCGCTTCAGATTTCGACTTCGCACCCCCAAAGGTAATGGAAGACACCGTTCCTGAGATCTTCAGTAAAAGGGGCTCTGATAGCGTCCGCAAAAAAATTAAAATGACAAAGAATGAGGATAAGAGCGCTGATATCACTGGTGAAGAAGATTTCGAGTAG

Protein Analysis

190

Amino Acids

21.69

Weight (kDa)

8.52

Isoelectric Point (pI)

51.54

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
zf-CW PF07496 24 - 70 2.8e-09 CW-type Zinc Finger
MBD PF01429 81 - 151 8.7e-17 Methyl-CpG binding domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0013011)

Species Orthologous Gene IDs
arabidopsis_thaliana AT3G63030
fragaria_vesca FvH4_2g24220
malus_domestica MD03G1271900.v1.1 MD11G1292500.v1.1
prunus_persica Prupe.8G248100_v2.0.a1
pyrus_communis pycom03g21650 pycom11g25890
rosa_chinensis RchiOBHm_Chr6g0292001
rosa_laevigata RLG00000012071
rosa_multiflora Rmu_sc0008666.1_g000005
rosa_roxburghii Rroxscaffold_7G00175500 Rroxscaffold_7G00178020
rosa_rugosa Rorug06G0222400
rosa_samantha Rh6AG336200 Rh6BG343600 Rh6CG349800 Rh6DG336600
rosa_wichuraiana Rw6G029270

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc16I TGCGCA 1 cut(s) 78
AciI CCGC 2 cut(s) 414, 499
AclWI GGATC 1 cut(s) 351
AcoI YGGCCR 1 cut(s) 7
AcuI CTGAAG 3 cut(s) 138, 402, 457
AfaI GTAC 3 cut(s) 128, 154, 218
AfeI AGCGCT 1 cut(s) 538
AjnI CCWGG 1 cut(s) 233
Alw26I GTCTC 1 cut(s) 277
AlwI GGATC 1 cut(s) 351
AlwNI CAGNNNCTG 1 cut(s) 234
Aor51HI AGCGCT 1 cut(s) 538
AoxI GGCC 1 cut(s) 7
ApeKI GCWGC 3 cut(s) 74, 203, 374
AspLEI GCGC 2 cut(s) 79, 539
AspS9I GGNCC 1 cut(s) 231
AsuC2I CCSGG 1 cut(s) 269
AsuHPI GGTGA 1 cut(s) 563
AsuNHI GCTAGC 1 cut(s) 224
AvaII GGWCC 1 cut(s) 231
BanII GRGCYC 1 cut(s) 488
BbsI GAAGAC 2 cut(s) 269, 459
BbvI GCAGC 3 cut(s) 61, 190, 386
BccI CCATC 1 cut(s) 364
BceAI ACGGC 1 cut(s) 22
BciT130I CCWGG 1 cut(s) 235
BciVI GTATCC 1 cut(s) 313
BcnI CCSGG 1 cut(s) 269
BcoDI GTCTC 1 cut(s) 277
BfaI CTAG 2 cut(s) 225, 297
BfoI RGCGCY 1 cut(s) 540
BfuI GTATCC 1 cut(s) 313
BglII AGATCT 1 cut(s) 468
BisI GCNGC 3 cut(s) 75, 204, 375
BlsI GCNGC 3 cut(s) 76, 205, 376
Bme1390I CCNGG 2 cut(s) 235, 269
Bme18I GGWCC 1 cut(s) 231
BmgT120I GGNCC 1 cut(s) 231
BmiI GGNNCC 1 cut(s) 159
BmrFI CCNGG 2 cut(s) 235, 269
BmsI GCATC 1 cut(s) 190
BmtI GCTAGC 1 cut(s) 228
BoxI GACNNNNGTC 1 cut(s) 236
BpiI GAAGAC 2 cut(s) 269, 459
BpuMI CCSGG 1 cut(s) 269
BsaAI YACGTR 1 cut(s) 324
BsaJI CCNNGG 3 cut(s) 234, 267, 339
Bse1I ACTGG 2 cut(s) 321, 553
BseBI CCWGG 1 cut(s) 235
BseDI CCNNGG 3 cut(s) 234, 267, 339
BseMII CTCAG 2 cut(s) 344, 456
BseNI ACTGG 2 cut(s) 321, 553
BseXI GCAGC 3 cut(s) 61, 190, 386
BshFI GGCC 1 cut(s) 9
BsiSI CCGG 1 cut(s) 269
BsmAI GTCTC 1 cut(s) 277
BsnI GGCC 1 cut(s) 9
Bsp1286I GDGCHC 1 cut(s) 488
Bsp143I GATC 3 cut(s) 253, 356, 468
BspACI CCGC 2 cut(s) 414, 499
BspANI GGCC 1 cut(s) 9
BspCNI CTCAG 2 cut(s) 345, 457
BspLI GGNNCC 1 cut(s) 159
BspOI GCTAGC 1 cut(s) 228
BspPI GGATC 1 cut(s) 351
BsrI ACTGG 2 cut(s) 321, 553
BssECI CCNNGG 3 cut(s) 234, 267, 339
BssMI GATC 3 cut(s) 253, 356, 468
BssT1I CCWWGG 1 cut(s) 339
Bst2UI CCWGG 1 cut(s) 235
Bst4CI ACNGT 3 cut(s) 221, 395, 460
Bst6I CTCTTC 1 cut(s) 117
BstBAI YACGTR 1 cut(s) 324
BstC8I GCNNGC 1 cut(s) 226
BstDEI CTNAG 2 cut(s) 353, 465
BstH2I RGCGCY 1 cut(s) 540
BstHHI GCGC 2 cut(s) 79, 539
BstKTI GATC 3 cut(s) 256, 359, 471
BstMAI GTCTC 1 cut(s) 277
BstMBI GATC 3 cut(s) 253, 356, 468
BstMWI GCNNNNNNNGC 1 cut(s) 492
BstNI CCWGG 1 cut(s) 235
BstPAI GACNNNNGTC 1 cut(s) 236
BstSCI CCNGG 2 cut(s) 233, 267
BstSNI TACGTA 1 cut(s) 324
BstV1I GCAGC 3 cut(s) 61, 190, 386
BstV2I GAAGAC 2 cut(s) 269, 459
BstX2I RGATCY 2 cut(s) 356, 468
BstYI RGATCY 2 cut(s) 356, 468
BsuI GTATCC 1 cut(s) 313
BsuRI GGCC 1 cut(s) 9
BtsI GCAGTG 1 cut(s) 86
BtsIMutI CAGTG 3 cut(s) 86, 226, 546
Cac8I GCNNGC 1 cut(s) 226
CaiI CAGNNNCTG 1 cut(s) 234
CfoI GCGC 2 cut(s) 79, 539
Cfr13I GGNCC 1 cut(s) 231
CseI GACGC 1 cut(s) 484
Csp6I GTAC 3 cut(s) 127, 153, 217
CviJI RGCY 5 cut(s) 9, 250, 296, 408, 486
CviKI_1 RGCY 5 cut(s) 9, 250, 296, 408, 486
CviQI GTAC 3 cut(s) 127, 153, 217
DdeI CTNAG 2 cut(s) 353, 465
DpnI GATC 3 cut(s) 255, 358, 470
DpnII GATC 3 cut(s) 253, 356, 468
DraI TTTAAA 1 cut(s) 384
EaeI YGGCCR 1 cut(s) 7
Eam1104I CTCTTC 1 cut(s) 117
EarI CTCTTC 1 cut(s) 117
Eco105I TACGTA 1 cut(s) 324
Eco130I CCWWGG 1 cut(s) 339
Eco24I GRGCYC 1 cut(s) 488
Eco32I GATATC 1 cut(s) 544
Eco47I GGWCC 1 cut(s) 231
Eco47III AGCGCT 1 cut(s) 538
Eco57I CTGAAG 3 cut(s) 138, 402, 457
EcoO109I RGGNCCY 1 cut(s) 231
EcoRII CCWGG 1 cut(s) 233
EcoRV GATATC 1 cut(s) 544
EcoT14I CCWWGG 1 cut(s) 339
EcoT38I GRGCYC 1 cut(s) 488
ErhI CCWWGG 1 cut(s) 339
FaiI YATR 4 cut(s) 70, 72, 330, 402
Fnu4HI GCNGC 3 cut(s) 75, 204, 375
FriOI GRGCYC 1 cut(s) 488
Fsp4HI GCNGC 3 cut(s) 75, 204, 375
FspBI CTAG 2 cut(s) 225, 297
FspI TGCGCA 1 cut(s) 78
GlaI GCGC 2 cut(s) 78, 538
GluI GCNGC 3 cut(s) 75, 204, 375
HaeII RGCGCY 1 cut(s) 540
HaeIII GGCC 1 cut(s) 9
HapII CCGG 1 cut(s) 269
HgaI GACGC 1 cut(s) 484
HhaI GCGC 2 cut(s) 79, 539
Hin6I GCGC 2 cut(s) 77, 537
HinP1I GCGC 2 cut(s) 77, 537
HinfI GANTC 1 cut(s) 109
HpaII CCGG 1 cut(s) 269
HphI GGTGA 1 cut(s) 563
Hpy188I TCNGA 4 cut(s) 118, 261, 421, 490
Hpy188III TCNNGA 4 cut(s) 139, 280, 360, 464
Hpy99I CGWCG 2 cut(s) 28, 31
HpyAV CCTTC 2 cut(s) 266, 398
HpyCH4III ACNGT 3 cut(s) 221, 395, 460
HpyCH4IV ACGT 2 cut(s) 23, 323
HpyCH4V TGCA 2 cut(s) 93, 171
HpyF10VI GCNNNNNNNGC 1 cut(s) 492
HpyF3I CTNAG 2 cut(s) 353, 465
HpySE526I ACGT 2 cut(s) 23, 323
HspAI GCGC 2 cut(s) 77, 537
Kzo9I GATC 3 cut(s) 253, 356, 468
LmnI GCTCC 1 cut(s) 141
Lsp1109I GCAGC 3 cut(s) 61, 190, 386
LweI GCATC 1 cut(s) 190
MaeI CTAG 2 cut(s) 225, 297
MaeII ACGT 2 cut(s) 23, 323
MaeIII GTNAC 3 cut(s) 83, 187, 194
MalI GATC 3 cut(s) 255, 358, 470
MboI GATC 3 cut(s) 253, 356, 468
MboII GAAGA 8 cut(s) 131, 134, 248, 274, 463, 464, 566, 569
MflI RGATCY 2 cut(s) 356, 468
MhlI GDGCHC 1 cut(s) 488
MluCI AATT 1 cut(s) 507
MnlI CCTC 2 cut(s) 124, 520
MseI TTAA 2 cut(s) 383, 510
MspI CCGG 1 cut(s) 269
MspR9I CCNGG 2 cut(s) 235, 269
MvaI CCWGG 1 cut(s) 235
MwoI GCNNNNNNNGC 1 cut(s) 492
NciI CCSGG 1 cut(s) 269
NdeII GATC 3 cut(s) 253, 356, 468
NheI GCTAGC 1 cut(s) 224
NlaIV GGNNCC 1 cut(s) 159
NmuCI GTSAC 3 cut(s) 83, 187, 194
NsbI TGCGCA 1 cut(s) 78
PcsI WCGNNNNNNNCGW 1 cut(s) 204
PfeI GAWTC 1 cut(s) 109
PkrI GCNGC 3 cut(s) 76, 205, 376
Ppu21I YACGTR 1 cut(s) 324
PpuMI RGGWCCY 1 cut(s) 231
PshAI GACNNNNGTC 1 cut(s) 236
Psp5II RGGWCCY 1 cut(s) 231
Psp6I CCWGG 1 cut(s) 233
PspGI CCWGG 1 cut(s) 233
PspN4I GGNNCC 1 cut(s) 159
PspPI GGNCC 1 cut(s) 231
PspPPI RGGWCCY 1 cut(s) 231
PstNI CAGNNNCTG 1 cut(s) 234
PsuI RGATCY 2 cut(s) 356, 468
RsaI GTAC 3 cut(s) 128, 154, 218
RsaNI GTAC 3 cut(s) 127, 153, 217
SaqAI TTAA 2 cut(s) 383, 510
SatI GCNGC 3 cut(s) 75, 204, 375
Sau3AI GATC 3 cut(s) 253, 356, 468
Sau96I GGNCC 1 cut(s) 231
ScrFI CCNGG 2 cut(s) 235, 269
SduI GDGCHC 1 cut(s) 488
SetI ASST 5 cut(s) 26, 236, 277, 326, 447
SfaNI GCATC 1 cut(s) 190
SinI GGWCC 1 cut(s) 231
SnaBI TACGTA 1 cut(s) 324
Sse9I AATT 1 cut(s) 507
SsiI CCGC 2 cut(s) 414, 499
SspMI CTAG 2 cut(s) 225, 297
StyD4I CCNGG 2 cut(s) 233, 267
StyI CCWWGG 1 cut(s) 339
TaaI ACNGT 3 cut(s) 221, 395, 460
TaiI ACGT 2 cut(s) 26, 326
TaqI TCGA 3 cut(s) 59, 426, 564
TasI AATT 1 cut(s) 507
TatI WGTACW 1 cut(s) 216
TfiI GAWTC 1 cut(s) 109
Tru1I TTAA 2 cut(s) 383, 510
Tru9I TTAA 2 cut(s) 383, 510
TscAI CASTG 3 cut(s) 86, 226, 553
TseFI GTSAC 3 cut(s) 83, 187, 194
TseI GCWGC 3 cut(s) 74, 203, 374
Tsp45I GTSAC 3 cut(s) 83, 187, 194
TspDTI ATGAA 2 cut(s) 101, 417
TspGWI ACGGA 1 cut(s) 170
TspRI CASTG 3 cut(s) 86, 226, 553
VpaK11BI GGWCC 1 cut(s) 231
XcmI CCANNNNNNNNNTGG 1 cut(s) 367
XspI CTAG 2 cut(s) 225, 297
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.