Rorug06G0222400

methyl-CpG-binding domain-containing protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000006
OG0013011
Physical Location & Seq
Forward (+)
35546196 .. 35548127
1932 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug06G0222400.1

Sequence Viewer

Length: 1080 bp
ATGGATGATTATGGAGCTTCTCCTGCTGGCTCTGCAGATGAGTCAAAGTCGACGTCTTGCCCAAGAGGACACTGGAGGCCCGCAGAAGATGAAAGACTCCGGCAACTCGTCGAACAGTACGGTGCCCAGAACTGGAACTCCATAGCAGAGAAGCTTCATGGAAGATCAGGGAAAAGTTGTAGGTTGAGGTGGTTTAATCAACTAGACCCAAGAATCAACAGAAGGCCGTTTAGTGAAGAAGAAGAAGAGAGGCTTCTAGCGGCTCATCGGATTCACGGAAACAAGTGGGCAATGATAGCAAGACTGTTTCCAGGTCGGACTGATAATGCCGTGAAGAATCACTGGCATGTTATCATGGCCAGAAGGCAAAGAGAGCAATCTAAGCTAATTTGTGGTGGAAACAAGAGAAGCTACCAAGATTTCTTAATCTCTAGTGACTCTATATCGAACACCTCCTCATCAATTTCCAACACTTTTGATTCAAGGAAATTGAAATCATCAACTGGGTTTGACCACAATCAGAACAGTACTACTACTATGTTTGAATTCAGGAACCCTGGTAAAGGTAGGGCAATGTCGTTCATGGAATCATCAAGGCCTAAAAGCTCTTCTTCTCATAATTGGAACTTTGCTTCAATGACAAGTTCATTATCAATTCCCAACTCGACATCTTCAGCGGGCTTCTTGAGAGGATCTGAAATATCTAATTACAAAGTTTCGGAGTGCTTGAAGGGTAATTCGGATCATCTTTTTGTTCATCGCATTAATGGGCAATATTCAAGTCCGGCATTCGTGTATGGCAGGTATAGAGGTTCCGGCAGTAGTAGTACTTTCGGGCTGCCAAATCCCAGGAGGGTTAATGTTCCAATTAATCCCTTTGGGTACCTCAGTAGTAGTACTAGTCCTCTTGGAGATATTCATGAGCAGCATGGAAGCAGGATGATATTGAAGAAAGAGTACCTGACGGCTGCATCATCTACTACATTTGAAGACCATCCTCATCCTCATCATGATTCATCCATCGAGCATAACAAGGAAGTTCCCTTCATAGATTTTCTTGGTGTGGGGATATCTAGCTAG

Protein Analysis

359

Amino Acids

40.32

Weight (kDa)

9.49

Isoelectric Point (pI)

59.46

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Myb_DNA-binding PF00249 22 - 68 1.3e-16 Myb-like DNA-binding domain
Myb_DNA-bind_6 PF13921 25 - 85 3.3e-19 Myb-like DNA-binding domain
Myb_DNA-binding PF00249 74 - 116 5.8e-16 Myb-like DNA-binding domain
Myb_DNA-bind_6 PF13921 77 - 125 6.7e-08 Myb-like DNA-binding domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0013011)

Species Orthologous Gene IDs
arabidopsis_thaliana AT3G63030
fragaria_vesca FvH4_2g24220
malus_domestica MD03G1271900.v1.1 MD11G1292500.v1.1
prunus_persica Prupe.8G248100_v2.0.a1
pyrus_communis pycom03g21650 pycom11g25890
rosa_chinensis RchiOBHm_Chr6g0292001
rosa_laevigata RLG00000012071
rosa_multiflora Rmu_sc0008666.1_g000005
rosa_roxburghii Rroxscaffold_7G00175500 Rroxscaffold_7G00178020
rosa_rugosa Rorug06G0222400
rosa_samantha Rh6AG336200 Rh6BG343600 Rh6CG349800 Rh6DG336600
rosa_wichuraiana Rw6G029270

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 56
Acc36I ACCTGC 1 cut(s) 792
Acc65I GGTACC 1 cut(s) 882
AccB1I GGYRCC 2 cut(s) 122, 882
AccI GTMKAC 1 cut(s) 50
AciI CCGC 3 cut(s) 81, 260, 677
AclWI GGATC 2 cut(s) 700, 750
AcoI YGGCCR 1 cut(s) 357
AcsI RAATTY 1 cut(s) 545
AcuI CTGAAG 1 cut(s) 657
AcyI GRCGYC 1 cut(s) 53
AfaI GTAC 6 cut(s) 119, 529, 829, 884, 898, 959
AfiI CCNNNNNNNGG 2 cut(s) 132, 563
AgsI TTSAA 8 cut(s) 483, 493, 545, 636, 730, 780, 949, 989
AhlI ACTAGT 1 cut(s) 899
AjnI CCWGG 3 cut(s) 310, 556, 848
AloI GAACNNNNNNTCC 2 cut(s) 122, 154
AluBI AGCT 6 cut(s) 17, 154, 385, 411, 606, 1077
AluI AGCT 6 cut(s) 17, 154, 385, 411, 606, 1077
AlwI GGATC 2 cut(s) 700, 750
AoxI GGCC 4 cut(s) 77, 224, 357, 596
ApeKI GCWGC 3 cut(s) 838, 925, 968
ApoI RAATTY 1 cut(s) 545
ArsI GACNNNNNNTTYG 2 cut(s) 38, 70
AseI ATTAAT 2 cut(s) 765, 870
Asp718I GGTACC 1 cut(s) 882
AspS9I GGNCC 1 cut(s) 78
BaeGI GKGCMC 1 cut(s) 127
BaeI ACNNNNGTAYC 2 cut(s) 874, 907
BalI TGGCCA 1 cut(s) 359
BanI GGYRCC 2 cut(s) 122, 882
BarI GAAGNNNNNNTAC 2 cut(s) 941, 973
BbsI GAAGAC 1 cut(s) 996
BbvI GCAGC 3 cut(s) 825, 937, 955
BccI CCATC 2 cut(s) 1002, 1028
BceAI ACGGC 3 cut(s) 211, 314, 981
BciT130I CCWGG 3 cut(s) 312, 558, 850
BcuI ACTAGT 1 cut(s) 899
BfaI CTAG 6 cut(s) 203, 257, 432, 900, 1074, 1078
BfmI CTRYAG 1 cut(s) 33
BfuAI ACCTGC 1 cut(s) 792
BisI GCNGC 4 cut(s) 261, 839, 926, 969
BlsI GCNGC 4 cut(s) 262, 840, 927, 970
BmcAI AGTACT 3 cut(s) 529, 829, 898
Bme1390I CCNGG 3 cut(s) 312, 558, 850
BmgT120I GGNCC 1 cut(s) 78
BmiI GGNNCC 4 cut(s) 124, 554, 814, 884
BmrFI CCNGG 3 cut(s) 312, 558, 850
BmrI ACTGGG 1 cut(s) 513
BmsI GCATC 1 cut(s) 980
BmuI ACTGGG 1 cut(s) 513
BpiI GAAGAC 1 cut(s) 996
BpmI CTGGAG 1 cut(s) 94
BpuEI CTTGAG 1 cut(s) 706
BsaHI GRCGYC 1 cut(s) 53
BsaJI CCNNGG 2 cut(s) 556, 848
BsaXI ACNNNNNCTCC 2 cut(s) 122, 152
Bsc4I CCNNNNNNNGG 2 cut(s) 132, 563
Bse1I ACTGG 4 cut(s) 77, 137, 347, 508
Bse3DI GCAATG 2 cut(s) 297, 579
BseBI CCWGG 3 cut(s) 312, 558, 850
BseDI CCNNGG 2 cut(s) 556, 848
BseGI GGATG 5 cut(s) 10, 945, 994, 1000, 1016
BseLI CCNNNNNNNGG 2 cut(s) 132, 563
BseMI GCAATG 2 cut(s) 297, 579
BseMII CTCAG 1 cut(s) 901
BseNI ACTGG 4 cut(s) 77, 137, 347, 508
BseRI GAGGAG 1 cut(s) 445
BseSI GKGCMC 1 cut(s) 127
BseXI GCAGC 3 cut(s) 825, 937, 955
BshFI GGCC 4 cut(s) 79, 226, 359, 598
BshNI GGYRCC 2 cut(s) 122, 882
BsiSI CCGG 3 cut(s) 100, 785, 816
BslI CCNNNNNNNGG 2 cut(s) 132, 563
BsmI GAATGC 1 cut(s) 788
BsnI GGCC 4 cut(s) 79, 226, 359, 598
Bsp1286I GDGCHC 1 cut(s) 127
Bsp143I GATC 3 cut(s) 164, 692, 742
BspACI CCGC 3 cut(s) 81, 260, 677
BspANI GGCC 4 cut(s) 79, 226, 359, 598
BspCNI CTCAG 1 cut(s) 900
BspHI TCATGA 2 cut(s) 919, 1009
BspLI GGNNCC 4 cut(s) 124, 554, 814, 884
BspMAI CTGCAG 1 cut(s) 37
BspMI ACCTGC 1 cut(s) 792
BspPI GGATC 2 cut(s) 700, 750
BspQI GCTCTTC 1 cut(s) 613
BspT107I GGYRCC 2 cut(s) 122, 882
BsrDI GCAATG 2 cut(s) 297, 579
BsrI ACTGG 4 cut(s) 77, 137, 347, 508
BssECI CCNNGG 2 cut(s) 556, 848
BssMI GATC 3 cut(s) 164, 692, 742
BssNI GRCGYC 1 cut(s) 53
Bst2UI CCWGG 3 cut(s) 312, 558, 850
Bst4CI ACNGT 4 cut(s) 117, 122, 306, 527
Bst6I CTCTTC 2 cut(s) 240, 613
BstACI GRCGYC 1 cut(s) 53
BstC8I GCNNGC 3 cut(s) 28, 81, 679
BstDEI CTNAG 2 cut(s) 381, 887
BstENI CCTNNNNNAGG 1 cut(s) 561
BstF5I GGATG 5 cut(s) 10, 945, 994, 1000, 1016
BstKTI GATC 3 cut(s) 167, 695, 745
BstMBI GATC 3 cut(s) 164, 692, 742
BstMWI GCNNNNNNNGC 5 cut(s) 23, 32, 296, 373, 382
BstNI CCWGG 3 cut(s) 312, 558, 850
BstNSI RCATGY 1 cut(s) 350
BstSCI CCNGG 3 cut(s) 310, 556, 848
BstSFI CTRYAG 1 cut(s) 33
BstSLI GKGCMC 1 cut(s) 127
BstV1I GCAGC 3 cut(s) 825, 937, 955
BstV2I GAAGAC 1 cut(s) 996
BstX2I RGATCY 1 cut(s) 692
BstYI RGATCY 1 cut(s) 692
BsuRI GGCC 4 cut(s) 79, 226, 359, 598
BtgZI GCGATG 1 cut(s) 743
BtsCI GGATG 5 cut(s) 10, 945, 994, 1000, 1016
BtsIMutI CAGTG 2 cut(s) 70, 340
BveI ACCTGC 1 cut(s) 792
Cac8I GCNNGC 3 cut(s) 28, 81, 679
CciI TCATGA 2 cut(s) 919, 1009
Cfr13I GGNCC 1 cut(s) 78
Csp6I GTAC 6 cut(s) 118, 528, 828, 883, 897, 958
CviAII CATG 7 cut(s) 158, 347, 355, 583, 920, 929, 1010
CviQI GTAC 6 cut(s) 118, 528, 828, 883, 897, 958
DdeI CTNAG 2 cut(s) 381, 887
DpnI GATC 3 cut(s) 166, 694, 744
DpnII GATC 3 cut(s) 164, 692, 742
EaeI YGGCCR 1 cut(s) 357
Eam1104I CTCTTC 2 cut(s) 240, 613
EarI CTCTTC 2 cut(s) 240, 613
Eco147I AGGCCT 1 cut(s) 598
Eco32I GATATC 1 cut(s) 1071
Eco57I CTGAAG 1 cut(s) 657
EcoNI CCTNNNNNAGG 1 cut(s) 561
EcoRI GAATTC 1 cut(s) 545
EcoRII CCWGG 3 cut(s) 310, 556, 848
EcoRV GATATC 1 cut(s) 1071
FaeI CATG 7 cut(s) 161, 350, 358, 586, 923, 932, 1013
FalI AAGNNNNNCTT 4 cut(s) 237, 269, 595, 627
FatI CATG 7 cut(s) 157, 346, 354, 582, 919, 928, 1009
FauI CCCGC 2 cut(s) 88, 670
FblI GTMKAC 1 cut(s) 50
Fnu4HI GCNGC 4 cut(s) 261, 839, 926, 969
FokI GGATG 5 cut(s) 17, 952, 981, 987, 1003
Fsp4HI GCNGC 4 cut(s) 261, 839, 926, 969
FspBI CTAG 6 cut(s) 203, 257, 432, 900, 1074, 1078
GluI GCNGC 4 cut(s) 261, 839, 926, 969
GsuI CTGGAG 1 cut(s) 94
HaeIII GGCC 4 cut(s) 79, 226, 359, 598
HapII CCGG 3 cut(s) 100, 785, 816
Hin1I GRCGYC 1 cut(s) 53
Hin1II CATG 7 cut(s) 161, 350, 358, 586, 923, 932, 1013
HincII GTYRAC 1 cut(s) 51
HindII GTYRAC 1 cut(s) 51
HindIII AAGCTT 1 cut(s) 152
HinfI GANTC 9 cut(s) 41, 96, 213, 271, 337, 437, 479, 587, 1013
HpaII CCGG 3 cut(s) 100, 785, 816
Hpy166II GTNNAC 1 cut(s) 51
Hpy188I TCNGA 6 cut(s) 270, 318, 522, 697, 721, 742
Hpy188III TCNNGA 4 cut(s) 550, 685, 920, 1010
Hpy8I GTNNAC 1 cut(s) 51
Hpy99I CGWCG 2 cut(s) 55, 113
HpyAV CCTTC 4 cut(s) 216, 357, 724, 1054
HpyCH4III ACNGT 4 cut(s) 117, 122, 306, 527
HpyCH4IV ACGT 1 cut(s) 53
HpyCH4V TGCA 2 cut(s) 35, 971
HpyF10VI GCNNNNNNNGC 5 cut(s) 23, 32, 296, 373, 382
HpyF3I CTNAG 2 cut(s) 381, 887
HpySE526I ACGT 1 cut(s) 53
Hsp92I GRCGYC 1 cut(s) 53
Hsp92II CATG 7 cut(s) 161, 350, 358, 586, 923, 932, 1013
KpnI GGTACC 1 cut(s) 886
Kzo9I GATC 3 cut(s) 164, 692, 742
LguI GCTCTTC 1 cut(s) 613
LmnI GCTCC 1 cut(s) 14
Lsp1109I GCAGC 3 cut(s) 825, 937, 955
LweI GCATC 1 cut(s) 980
MaeI CTAG 6 cut(s) 203, 257, 432, 900, 1074, 1078
MaeII ACGT 1 cut(s) 53
MaeIII GTNAC 1 cut(s) 434
MalI GATC 3 cut(s) 166, 694, 744
MboI GATC 3 cut(s) 164, 692, 742
MflI RGATCY 1 cut(s) 692
MhlI GDGCHC 1 cut(s) 127
MlsI TGGCCA 1 cut(s) 359
MluCI AATT 9 cut(s) 387, 462, 488, 545, 619, 654, 706, 736, 867
MluNI TGGCCA 1 cut(s) 359
MlyI GAGTC 3 cut(s) 50, 90, 431
MmeI TCCRAC 2 cut(s) 296, 492
Mox20I TGGCCA 1 cut(s) 359
MscI TGGCCA 1 cut(s) 359
MseI TTAA 5 cut(s) 195, 425, 765, 858, 870
MslI CAYNNNNRTG 1 cut(s) 345
Msp20I TGGCCA 1 cut(s) 359
MspA1I CMGCKG 1 cut(s) 677
MspI CCGG 3 cut(s) 100, 785, 816
MspR9I CCNGG 3 cut(s) 312, 558, 850
Mva1269I GAATGC 1 cut(s) 788
MvaI CCWGG 3 cut(s) 312, 558, 850
MwoI GCNNNNNNNGC 5 cut(s) 23, 32, 296, 373, 382
NdeII GATC 3 cut(s) 164, 692, 742
NlaIII CATG 7 cut(s) 161, 350, 358, 586, 923, 932, 1013
NlaIV GGNNCC 4 cut(s) 124, 554, 814, 884
NmuCI GTSAC 1 cut(s) 434
NspI RCATGY 1 cut(s) 350
PagI TCATGA 2 cut(s) 919, 1009
PceI AGGCCT 1 cut(s) 598
PciSI GCTCTTC 1 cut(s) 613
PctI GAATGC 1 cut(s) 788
PfeI GAWTC 6 cut(s) 213, 271, 337, 479, 587, 1013
PkrI GCNGC 4 cut(s) 262, 840, 927, 970
PleI GAGTC 3 cut(s) 49, 90, 431
PpsI GAGTC 3 cut(s) 49, 90, 431
PshBI ATTAAT 2 cut(s) 765, 870
Psp6I CCWGG 3 cut(s) 310, 556, 848
PspGI CCWGG 3 cut(s) 310, 556, 848
PspN4I GGNNCC 4 cut(s) 124, 554, 814, 884
PspPI GGNCC 1 cut(s) 78
PstI CTGCAG 1 cut(s) 37
PsuI RGATCY 1 cut(s) 692
RsaI GTAC 6 cut(s) 119, 529, 829, 884, 898, 959
RsaNI GTAC 6 cut(s) 118, 528, 828, 883, 897, 958
RseI CAYNNNNRTG 1 cut(s) 345
SalI GTCGAC 1 cut(s) 49
SapI GCTCTTC 1 cut(s) 613
SaqAI TTAA 5 cut(s) 195, 425, 765, 858, 870
SatI GCNGC 4 cut(s) 261, 839, 926, 969
Sau3AI GATC 3 cut(s) 164, 692, 742
Sau96I GGNCC 1 cut(s) 78
ScaI AGTACT 3 cut(s) 529, 829, 898
SchI GAGTC 3 cut(s) 50, 90, 431
ScrFI CCNGG 3 cut(s) 312, 558, 850
SduI GDGCHC 1 cut(s) 127
SfaNI GCATC 1 cut(s) 980
SfcI CTRYAG 1 cut(s) 33
SmiMI CAYNNNNRTG 1 cut(s) 345
SmlI CTYRAG 1 cut(s) 685
SmoI CTYRAG 1 cut(s) 685
SpeI ACTAGT 1 cut(s) 899
Sse9I AATT 9 cut(s) 387, 462, 488, 545, 619, 654, 706, 736, 867
SseBI AGGCCT 1 cut(s) 598
SsiI CCGC 3 cut(s) 81, 260, 677
SspI AATATT 1 cut(s) 776
SspMI CTAG 6 cut(s) 203, 257, 432, 900, 1074, 1078
StuI AGGCCT 1 cut(s) 598
StyD4I CCNGG 3 cut(s) 310, 556, 848
TaaI ACNGT 4 cut(s) 117, 122, 306, 527
TaiI ACGT 1 cut(s) 56
TaqI TCGA 5 cut(s) 50, 111, 446, 665, 1023
TasI AATT 9 cut(s) 387, 462, 488, 545, 619, 654, 706, 736, 867
TatI WGTACW 3 cut(s) 527, 827, 896
TauI GCSGC 1 cut(s) 263
TfiI GAWTC 6 cut(s) 213, 271, 337, 479, 587, 1013
Tru1I TTAA 5 cut(s) 195, 425, 765, 858, 870
Tru9I TTAA 5 cut(s) 195, 425, 765, 858, 870
TscAI CASTG 2 cut(s) 77, 347
TseFI GTSAC 1 cut(s) 434
TseI GCWGC 3 cut(s) 838, 925, 968
Tsp45I GTSAC 1 cut(s) 434
TspDTI ATGAA 8 cut(s) 105, 146, 571, 636, 746, 908, 1005, 1036
TspGWI ACGGA 1 cut(s) 291
TspRI CASTG 2 cut(s) 77, 347
VspI ATTAAT 2 cut(s) 765, 870
XagI CCTNNNNNAGG 1 cut(s) 561
XapI RAATTY 1 cut(s) 545
XceI RCATGY 1 cut(s) 350
XcmI CCANNNNNNNNNTGG 1 cut(s) 69
XmiI GTMKAC 1 cut(s) 50
XspI CTAG 6 cut(s) 203, 257, 432, 900, 1074, 1078
ZraI GACGTC 1 cut(s) 54
ZrmI AGTACT 3 cut(s) 529, 829, 898
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.