MD11G1292500.v1.1

methyl-CpG-binding domain-containing protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr11
OG0013011
Physical Location & Seq
Reverse (-)
41207582 .. 41210047
2466 bp
Loading structure...
UTR
Exon/CDS
Intron
MD11G1292500.v1.1.491

Sequence Viewer

Length: 723 bp
ATGTGGACAATTGAAGGGTCAAACCGTCATTCTGGTCCAAAGATAAACGGAGGAACCGAATTGGGCACATATGGTGTCATCCTCCTCCTCGGGCTGTTCGGCAAGATTCGGAAAAAAGAGAGAATTTTTCAGAAGCCGGCGAAGCACGAAATGGACGGCCGAGAAACGACTAAAACGTCGTCGTCCAAGAAACCAAATATAGGGACAAGTTCGATTGATGTATATGCTGCACAATGTGCAGAATGCAAGAAATGGAGAGTGATTCATTCAGAAGAAGAGTTTGAGGAAATCAGGAGCAAAATCAGTACGCAACCCTTTTATTGCAACAAGAAATCTGGTGTGACTTGTGACGATGTTGCCGATATGGAGTACAATGCTACTAGGACCTGGGTCATTGATAAGCCTGACCTTCCGAAGACCCCAGAAGGTTTCAAGAGGCACTTGGTGGTTAGAAAAGATTATTCCAAACTGGATGCGTATTATATTACACCCAAGGGGAAAAGATTGAGATCACAAAATCAGGTGGCAGCGTTTTTAAAAGAAAACCCCATGTTTGAAGGCATATCCGCTTCAGATTTCGACTTCGCAGCCCCAAAGGTAATGGAAGACACCGTTCCTGAGATTTTTGGTAAAAAGGGTTCTGATAGCAGCCGCAAAAAAATTAAAAAGAAAAAGAATGAGGATCAAAGTGCTGTTATCACTGGTAAAGAAGATGTTGAGTAG

Protein Analysis

241

Amino Acids

27.14

Weight (kDa)

9.22

Isoelectric Point (pI)

39.12

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
zf-CW PF07496 75 - 118 1.2e-07 CW-type Zinc Finger
MBD PF01429 132 - 202 4.7e-16 Methyl-CpG binding domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Orthologous Genes (Group: OG0013011)

Species Orthologous Gene IDs
arabidopsis_thaliana AT3G63030
fragaria_vesca FvH4_2g24220
malus_domestica MD03G1271900.v1.1 MD11G1292500.v1.1
prunus_persica Prupe.8G248100_v2.0.a1
pyrus_communis pycom03g21650 pycom11g25890
rosa_chinensis RchiOBHm_Chr6g0292001
rosa_laevigata RLG00000012071
rosa_multiflora Rmu_sc0008666.1_g000005
rosa_roxburghii Rroxscaffold_7G00175500 Rroxscaffold_7G00178020
rosa_rugosa Rorug06G0222400
rosa_samantha Rh6AG336200 Rh6BG343600 Rh6CG349800 Rh6DG336600
rosa_wichuraiana Rw6G029270

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 175
AciI CCGC 2 cut(s) 567, 652
AclWI GGATC 1 cut(s) 690
AcoI YGGCCR 1 cut(s) 157
AcsI RAATTY 1 cut(s) 123
AcuI CTGAAG 1 cut(s) 555
AdeI CACNNNGTG 2 cut(s) 236, 445
AfaI GTAC 2 cut(s) 307, 371
AfiI CCNNNNNNNGG 1 cut(s) 200
AgsI TTSAA 3 cut(s) 14, 433, 557
AjnI CCWGG 1 cut(s) 386
AlwI GGATC 1 cut(s) 690
Ama87I CYCGRG 1 cut(s) 89
AoxI GGCC 1 cut(s) 157
ApeKI GCWGC 4 cut(s) 227, 527, 587, 648
ApoI RAATTY 1 cut(s) 123
AspS9I GGNCC 2 cut(s) 35, 384
AvaI CYCGRG 1 cut(s) 89
AvaII GGWCC 2 cut(s) 35, 384
BaeGI GKGCMC 1 cut(s) 68
BbsI GAAGAC 2 cut(s) 422, 612
BbvI GCAGC 4 cut(s) 214, 539, 599, 660
BceAI ACGGC 1 cut(s) 172
BciT130I CCWGG 1 cut(s) 388
BfaI CTAG 1 cut(s) 381
BisI GCNGC 5 cut(s) 228, 528, 588, 649, 652
BlsI GCNGC 5 cut(s) 229, 529, 589, 650, 653
Bme1390I CCNGG 1 cut(s) 388
Bme18I GGWCC 2 cut(s) 35, 384
BmeT110I CYCGRG 1 cut(s) 89
BmgT120I GGNCC 2 cut(s) 35, 384
BmiI GGNNCC 1 cut(s) 55
BmrFI CCNGG 1 cut(s) 388
BmsI GCATC 1 cut(s) 463
BoxI GACNNNNGTC 1 cut(s) 389
BpiI GAAGAC 2 cut(s) 422, 612
BsaBI GATNNNNATC 1 cut(s) 508
BsaJI CCNNGG 3 cut(s) 88, 387, 492
Bsc4I CCNNNNNNNGG 1 cut(s) 200
Bse118I RCCGGY 1 cut(s) 136
Bse1I ACTGG 2 cut(s) 474, 706
Bse8I GATNNNNATC 1 cut(s) 508
BseBI CCWGG 1 cut(s) 388
BseDI CCNNGG 3 cut(s) 88, 387, 492
BseGI GGATG 2 cut(s) 78, 478
BseJI GATNNNNATC 1 cut(s) 508
BseLI CCNNNNNNNGG 1 cut(s) 200
BseMII CTCAG 1 cut(s) 609
BseNI ACTGG 2 cut(s) 474, 706
BseRI GAGGAG 2 cut(s) 74, 77
BseSI GKGCMC 1 cut(s) 68
BseX3I CGGCCG 1 cut(s) 157
BseXI GCAGC 4 cut(s) 214, 539, 599, 660
BsgI GTGCAG 2 cut(s) 213, 258
Bsh1285I CGRYCG 1 cut(s) 160
BshFI GGCC 1 cut(s) 159
BsiEI CGRYCG 1 cut(s) 160
BsiHKCI CYCGRG 1 cut(s) 89
BsiSI CCGG 1 cut(s) 137
BslFI GGGAC 1 cut(s) 217
BslI CCNNNNNNNGG 1 cut(s) 200
BsmFI GGGAC 1 cut(s) 217
BsmI GAATGC 1 cut(s) 248
BsnI GGCC 1 cut(s) 159
BsoBI CYCGRG 1 cut(s) 89
Bsp1286I GDGCHC 1 cut(s) 68
Bsp143I GATC 2 cut(s) 509, 682
BspACI CCGC 2 cut(s) 567, 652
BspANI GGCC 1 cut(s) 159
BspCNI CTCAG 1 cut(s) 610
BspLI GGNNCC 1 cut(s) 55
BspPI GGATC 1 cut(s) 690
BsrFI RCCGGY 1 cut(s) 136
BsrI ACTGG 2 cut(s) 474, 706
BssAI RCCGGY 1 cut(s) 136
BssECI CCNNGG 3 cut(s) 88, 387, 492
BssMI GATC 2 cut(s) 509, 682
BssT1I CCWWGG 1 cut(s) 492
Bst2UI CCWGG 1 cut(s) 388
Bst4CI ACNGT 2 cut(s) 26, 613
Bst6I CTCTTC 1 cut(s) 270
BstAPI GCANNNNNTGC 1 cut(s) 236
BstC8I GCNNGC 1 cut(s) 138
BstDEI CTNAG 1 cut(s) 618
BstF5I GGATG 2 cut(s) 78, 478
BstKTI GATC 2 cut(s) 512, 685
BstMBI GATC 2 cut(s) 509, 682
BstMCI CGRYCG 1 cut(s) 160
BstMWI GCNNNNNNNGC 2 cut(s) 142, 236
BstNI CCWGG 1 cut(s) 388
BstPAI GACNNNNGTC 1 cut(s) 389
BstSCI CCNGG 1 cut(s) 386
BstSLI GKGCMC 1 cut(s) 68
BstV1I GCAGC 4 cut(s) 214, 539, 599, 660
BstV2I GAAGAC 2 cut(s) 422, 612
BstZI CGGCCG 1 cut(s) 157
BsuRI GGCC 1 cut(s) 159
BtsCI GGATG 2 cut(s) 78, 478
BtsIMutI CAGTG 1 cut(s) 699
Cac8I GCNNGC 1 cut(s) 138
Cfr10I RCCGGY 1 cut(s) 136
Cfr13I GGNCC 2 cut(s) 35, 384
Csp6I GTAC 2 cut(s) 306, 370
CviAII CATG 1 cut(s) 550
CviJI RGCY 6 cut(s) 94, 136, 159, 403, 590, 651
CviKI_1 RGCY 6 cut(s) 94, 136, 159, 403, 590, 651
CviQI GTAC 2 cut(s) 306, 370
DdeI CTNAG 1 cut(s) 618
DpnI GATC 2 cut(s) 511, 684
DpnII GATC 2 cut(s) 509, 682
DraI TTTAAA 1 cut(s) 537
DraIII CACNNNGTG 2 cut(s) 236, 445
DrdI GACNNNNNNGTC 1 cut(s) 175
DseDI GACNNNNNNGTC 1 cut(s) 175
EaeI YGGCCR 1 cut(s) 157
EagI CGGCCG 1 cut(s) 157
Eam1104I CTCTTC 1 cut(s) 270
EarI CTCTTC 1 cut(s) 270
EclXI CGGCCG 1 cut(s) 157
Eco130I CCWWGG 1 cut(s) 492
Eco47I GGWCC 2 cut(s) 35, 384
Eco52I CGGCCG 1 cut(s) 157
Eco57I CTGAAG 1 cut(s) 555
Eco88I CYCGRG 1 cut(s) 89
EcoO109I RGGNCCY 1 cut(s) 384
EcoRII CCWGG 1 cut(s) 386
EcoT14I CCWWGG 1 cut(s) 492
ErhI CCWWGG 1 cut(s) 492
FaeI CATG 1 cut(s) 553
FaiI YATR 9 cut(s) 70, 72, 200, 223, 225, 365, 483, 551, 563
FalI AAGNNNNNCTT 2 cut(s) 425, 457
FaqI GGGAC 1 cut(s) 217
FatI CATG 1 cut(s) 549
FauNDI CATATG 1 cut(s) 70
Fnu4HI GCNGC 5 cut(s) 228, 528, 588, 649, 652
FokI GGATG 2 cut(s) 65, 485
Fsp4HI GCNGC 5 cut(s) 228, 528, 588, 649, 652
FspBI CTAG 1 cut(s) 381
GluI GCNGC 5 cut(s) 228, 528, 588, 649, 652
HaeIII GGCC 1 cut(s) 159
HapII CCGG 1 cut(s) 137
Hin1II CATG 1 cut(s) 553
HinfI GANTC 2 cut(s) 106, 262
HpaII CCGG 1 cut(s) 137
Hpy166II GTNNAC 1 cut(s) 6
Hpy188I TCNGA 6 cut(s) 111, 132, 271, 414, 574, 643
Hpy188III TCNNGA 3 cut(s) 292, 433, 617
Hpy8I GTNNAC 1 cut(s) 6
Hpy99I CGWCG 2 cut(s) 181, 184
HpyAV CCTTC 4 cut(s) 8, 419, 419, 551
HpyCH4III ACNGT 2 cut(s) 26, 613
HpyCH4IV ACGT 1 cut(s) 176
HpyCH4V TGCA 4 cut(s) 230, 239, 246, 324
HpyF10VI GCNNNNNNNGC 2 cut(s) 142, 236
HpyF3I CTNAG 1 cut(s) 618
HpySE526I ACGT 1 cut(s) 176
Hsp92II CATG 1 cut(s) 553
KroI GCCGGC 1 cut(s) 136
KroNI GCCGGC 1 cut(s) 138
Kzo9I GATC 2 cut(s) 509, 682
LmnI GCTCC 1 cut(s) 294
Lsp1109I GCAGC 4 cut(s) 214, 539, 599, 660
LweI GCATC 1 cut(s) 463
MaeI CTAG 1 cut(s) 381
MaeII ACGT 1 cut(s) 176
MaeIII GTNAC 2 cut(s) 340, 347
MalI GATC 2 cut(s) 511, 684
MboI GATC 2 cut(s) 509, 682
MboII GAAGA 5 cut(s) 284, 287, 427, 617, 722
MfeI CAATTG 1 cut(s) 9
MhlI GDGCHC 1 cut(s) 68
MluCI AATT 4 cut(s) 9, 59, 123, 660
MnlI CCTC 7 cut(s) 44, 92, 95, 98, 277, 429, 673
MroNI GCCGGC 1 cut(s) 136
MseI TTAA 2 cut(s) 536, 663
MspI CCGG 1 cut(s) 137
MspR9I CCNGG 1 cut(s) 388
MunI CAATTG 1 cut(s) 9
Mva1269I GAATGC 1 cut(s) 248
MvaI CCWGG 1 cut(s) 388
MwoI GCNNNNNNNGC 2 cut(s) 142, 236
NaeI GCCGGC 1 cut(s) 138
NdeI CATATG 1 cut(s) 70
NdeII GATC 2 cut(s) 509, 682
NgoMIV GCCGGC 1 cut(s) 136
NlaIII CATG 1 cut(s) 553
NlaIV GGNNCC 1 cut(s) 55
NmeAIII GCCGAG 1 cut(s) 185
NmuCI GTSAC 2 cut(s) 340, 347
PcsI WCGNNNNNNNCGW 3 cut(s) 54, 173, 357
PctI GAATGC 1 cut(s) 248
PdiI GCCGGC 1 cut(s) 138
PfeI GAWTC 2 cut(s) 106, 262
PkrI GCNGC 5 cut(s) 229, 529, 589, 650, 653
PpuMI RGGWCCY 1 cut(s) 384
PshAI GACNNNNGTC 1 cut(s) 389
Psp5II RGGWCCY 1 cut(s) 384
Psp6I CCWGG 1 cut(s) 386
PspGI CCWGG 1 cut(s) 386
PspN4I GGNNCC 1 cut(s) 55
PspPI GGNCC 2 cut(s) 35, 384
PspPPI RGGWCCY 1 cut(s) 384
PsrI GAACNNNNNNTAC 2 cut(s) 622, 654
RsaI GTAC 2 cut(s) 307, 371
RsaNI GTAC 2 cut(s) 306, 370
SaqAI TTAA 2 cut(s) 536, 663
SatI GCNGC 5 cut(s) 228, 528, 588, 649, 652
Sau3AI GATC 2 cut(s) 509, 682
Sau96I GGNCC 2 cut(s) 35, 384
ScrFI CCNGG 1 cut(s) 388
SduI GDGCHC 1 cut(s) 68
SetI ASST 6 cut(s) 179, 389, 411, 430, 525, 600
SfaNI GCATC 1 cut(s) 463
SinI GGWCC 2 cut(s) 35, 384
Sse9I AATT 4 cut(s) 9, 59, 123, 660
SsiI CCGC 2 cut(s) 567, 652
SspMI CTAG 1 cut(s) 381
StyD4I CCNGG 1 cut(s) 386
StyI CCWWGG 1 cut(s) 492
TaaI ACNGT 2 cut(s) 26, 613
TaiI ACGT 1 cut(s) 179
TaqI TCGA 2 cut(s) 212, 579
TasI AATT 4 cut(s) 9, 59, 123, 660
TatI WGTACW 1 cut(s) 369
TauI GCSGC 1 cut(s) 654
TfiI GAWTC 2 cut(s) 106, 262
Tru1I TTAA 2 cut(s) 536, 663
Tru9I TTAA 2 cut(s) 536, 663
TscAI CASTG 1 cut(s) 706
TseFI GTSAC 2 cut(s) 340, 347
TseI GCWGC 4 cut(s) 227, 527, 587, 648
Tsp45I GTSAC 2 cut(s) 340, 347
TspDTI ATGAA 1 cut(s) 254
TspGWI ACGGA 1 cut(s) 63
TspRI CASTG 1 cut(s) 706
VpaK11BI GGWCC 2 cut(s) 35, 384
XapI RAATTY 1 cut(s) 123
XspI CTAG 1 cut(s) 381
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.