MD03G1277700.v1.1

RING finger and CHY zinc finger domain-containing protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr03
OG0013924
Physical Location & Seq
Reverse (-)
35985022 .. 35988781
3760 bp
Loading structure...
UTR
Exon/CDS
Intron
MD03G1277700.v1.1.491

Sequence Viewer

Length: 819 bp
ATGGGGGAAGACTCTTCGGCTGCGCCGCCCACTCTTACCCGCCAAGATTTCGGAAAGATGGAATACGGATGCGACCATTATCGGAGACGATGCAAGATTCGTGCCCCTTGCTGCGACCAGATTTTCCCCTGCCGCCACTGCCACAACGAAGCCGCGAGCTCGCTAAACAACCCGAAAGACCGCCATGAGCTTGTCAGGCACGATGTCAAGCAGGTTGTATGCTCAGTCTGCAACACCGAGCAACAGGTTGCTCAGCTTTGTTCTAACTGCGGTGTCAACATGGGGGAATACTATTGCGACATTTGCAAGTTCTTCGATGACGAAACTCACAAGGGACAATTTCATTGCAATGAGTGCGGAATTTGTAGAGTTGGTGGTAGCGAGAATTTCTTCCATTGTCAGAAGTGTGGTTCTTGCTATGCAGTGGGTCTGCGTGATAACCACTTATGTGTAGAAGACTCAATGAAGAATCACTGCCCTGTTTGCTATGAGTATCTTTTTGACTCGGTTAAAGTCACAAATATTATGAAGTGTGGACACACAATGCATTCAGATTGCTTCATTGAGATGCAAAACCAAAATCAATATCGCTGTCCCATTTGCTCCAAGACAGTGCTCGAAATGGGTCATATGTGGAACTTGTTGGATGCAGAAATTGAAGCCACTGCTATGCCTGCGGAATACCAATATGAGGTATCAATTCTTTGCAACGATTGCAACACAACTAGCAACGCCCCCTTTCACATTTTTGGACACAAGTGCGGCAACTGCCATTCATACAACACCAGAGTAATAGATAGACCTACAAACCATCAATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000151 GO:0000731 GO:0002039 GO:0003674 GO:0003824 GO:0004842 GO:0005488 GO:0005515 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005654 GO:0005737 GO:0005829 GO:0006139 GO:0006259 GO:0006281 GO:0006301 GO:0006464 GO:0006508 GO:0006511 GO:0006725 GO:0006807 GO:0006950 GO:0006974 GO:0008150 GO:0008152 GO:0008270 GO:0009056 GO:0009057 GO:0009058 GO:0009059 GO:0009893 GO:0009894 GO:0009896 GO:0009987 GO:0010604 GO:0016567 GO:0016604 GO:0016607 GO:0016740 GO:0018130 GO:0019222 GO:0019438 GO:0019538 GO:0019787 GO:0019941 GO:0019985 GO:0030162 GO:0030163 GO:0031323 GO:0031325 GO:0031329 GO:0031331 GO:0031396 GO:0031398 GO:0031399 GO:0031401 GO:0031974 GO:0031981 GO:0032268 GO:0032270 GO:0032434 GO:0032436 GO:0032446 GO:0032991 GO:0033554 GO:0034641 GO:0034645 GO:0034654 GO:0036211 GO:0042176 GO:0042802 GO:0042803 GO:0043167 GO:0043169 GO:0043170 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043233 GO:0043412 GO:0043632 GO:0044237 GO:0044238 GO:0044248 GO:0044249 GO:0044257 GO:0044260 GO:0044265 GO:0044267 GO:0044271 GO:0044422 GO:0044424 GO:0044428 GO:0044444 GO:0044446 GO:0044451 GO:0044464 GO:0045732 GO:0045862 GO:0046483 GO:0046872 GO:0046914 GO:0046983 GO:0048518 GO:0048522 GO:0050789 GO:0050794 GO:0050896 GO:0051171 GO:0051173 GO:0051246 GO:0051247 GO:0051603 GO:0051716 GO:0051865 GO:0060255 GO:0061136 GO:0065007 GO:0070013 GO:0070647 GO:0070987 GO:0071704 GO:0071897 GO:0080090 GO:0090304 GO:0140096 GO:1901360 GO:1901362 GO:1901564 GO:1901565 GO:1901575 GO:1901576 GO:1901800 GO:1902494 GO:1903050 GO:1903052 GO:1903320 GO:1903322 GO:1903362 GO:1903364 GO:1990234 GO:2000058 GO:2000060
KEGG Pathways
Metabolic & Signaling
ko04115 ko04120 ko05162 map04115 map04120 map05162
Pfam Domains
Protein Families
zf-C3HC4_2 zf-CHY zf-RING_2 zf-RING_UBOX zinc_ribbon_6

Protein Analysis

273

Amino Acids

31.08

Weight (kDa)

6.07

Isoelectric Point (pI)

49.34

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
zf-CHY PF05495 24 - 97 1.9e-20 CHY zinc finger
zf-RING_2 PF13639 157 - 201 5.3e-08 Ring finger domain
zf-RING_5 PF14634 158 - 203 6.3e-06 zinc-RING finger domain
zf-C3HC4_2 PF13923 159 - 201 2e-07 Zinc finger, C3HC4 type (RING finger)
zf-C3HC4 PF00097 159 - 201 4.5e-07 Zinc finger, C3HC4 type (RING finger)
zinc_ribbon_6 PF14599 207 - 263 2.9e-24 Zinc-ribbon
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0013924)

Species Orthologous Gene IDs
arabidopsis_thaliana AT3G62970 AT3G62970 AT3G62970
fragaria_vesca FvH4_2g24920
malus_domestica MD03G1277700.v1.1
prunus_persica Prupe.8G254000_v2.0.a1
pyrus_communis pycom03g22110
rosa_chinensis RchiOBHm_Chr6g0292841
rosa_laevigata RLG00000011988
rosa_multiflora Rmu_sc0000639.1_g000021
rosa_roxburghii Rroxscaffold_7G00174660
rosa_rugosa Rorug06G0230100
rosa_samantha Rh6AG343400 Rh6BG350600 Rh6CG357600 Rh6DG343500
rosa_wichuraiana Rw6G029950

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 202
AccII CGCG 1 cut(s) 155
AciI CCGC 9 cut(s) 26, 40, 133, 153, 181, 270, 357, 677, 762
AcsI RAATTY 2 cut(s) 360, 385
AfiI CCNNNNNNNGG 1 cut(s) 691
AgsI TTSAA 1 cut(s) 659
AleI CACNNNNGTG 1 cut(s) 447
AluBI AGCT 3 cut(s) 159, 190, 256
AluI AGCT 3 cut(s) 159, 190, 256
Alw21I GWGCWC 2 cut(s) 161, 618
Alw26I GTCTC 1 cut(s) 79
ApeKI GCWGC 2 cut(s) 20, 111
ApoI RAATTY 2 cut(s) 360, 385
Asp700I GAANNNNTTC 1 cut(s) 389
AspLEI GCGC 1 cut(s) 25
BaeGI GKGCMC 1 cut(s) 106
BanII GRGCYC 1 cut(s) 161
BbsI GAAGAC 2 cut(s) 15, 462
Bbv12I GWGCWC 2 cut(s) 161, 618
BbvI GCAGC 2 cut(s) 7, 98
BccI CCATC 1 cut(s) 52
BcgI CGANNNNNNTGC 2 cut(s) 295, 329
BcoDI GTCTC 1 cut(s) 79
BfaI CTAG 1 cut(s) 726
BfuAI ACCTGC 1 cut(s) 202
BisI GCNGC 6 cut(s) 21, 26, 112, 133, 153, 763
BlpI GCTNAGC 1 cut(s) 252
BlsI GCNGC 6 cut(s) 22, 27, 113, 134, 154, 764
BmsI GCATC 4 cut(s) 59, 80, 558, 637
BpiI GAAGAC 2 cut(s) 15, 462
Bpu1102I GCTNAGC 1 cut(s) 252
Bsc4I CCNNNNNNNGG 1 cut(s) 691
Bse3DI GCAATG 2 cut(s) 343, 355
BseGI GGATG 2 cut(s) 74, 652
BseLI CCNNNNNNNGG 1 cut(s) 691
BseMI GCAATG 2 cut(s) 343, 355
BseMII CTCAG 2 cut(s) 237, 266
BseSI GKGCMC 1 cut(s) 106
BseXI GCAGC 2 cut(s) 7, 98
Bsh1236I CGCG 1 cut(s) 155
BsiHKAI GWGCWC 2 cut(s) 161, 618
BslFI GGGAC 2 cut(s) 348, 579
BslI CCNNNNNNNGG 1 cut(s) 691
BsmAI GTCTC 1 cut(s) 79
BsmBI CGTCTC 1 cut(s) 79
BsmFI GGGAC 2 cut(s) 348, 579
BsmI GAATGC 1 cut(s) 547
Bsp1286I GDGCHC 3 cut(s) 106, 161, 618
Bsp1720I GCTNAGC 1 cut(s) 252
BspACI CCGC 9 cut(s) 26, 40, 133, 153, 181, 270, 357, 677, 762
BspCNI CTCAG 2 cut(s) 236, 265
BspFNI CGCG 1 cut(s) 155
BspMI ACCTGC 1 cut(s) 202
BsrDI GCAATG 2 cut(s) 343, 355
Bst4CI ACNGT 1 cut(s) 613
Bst6I CTCTTC 1 cut(s) 19
BstAPI GCANNNNNTGC 2 cut(s) 354, 714
BstC8I GCNNGC 3 cut(s) 157, 161, 675
BstDEI CTNAG 2 cut(s) 223, 252
BstF5I GGATG 2 cut(s) 74, 652
BstFNI CGCG 1 cut(s) 155
BstHHI GCGC 1 cut(s) 25
BstMAI GTCTC 1 cut(s) 79
BstMWI GCNNNNNNNGC 9 cut(s) 138, 196, 228, 303, 354, 483, 674, 714, 768
BstSLI GKGCMC 1 cut(s) 106
BstUI CGCG 1 cut(s) 155
BstV1I GCAGC 2 cut(s) 7, 98
BstV2I GAAGAC 2 cut(s) 15, 462
BtsCI GGATG 2 cut(s) 74, 652
BtsI GCAGTG 4 cut(s) 136, 429, 472, 663
BtsIMutI CAGTG 5 cut(s) 136, 429, 472, 618, 663
BveI ACCTGC 1 cut(s) 202
Cac8I GCNNGC 3 cut(s) 157, 161, 675
CfoI GCGC 1 cut(s) 25
CviAII CATG 2 cut(s) 185, 280
CviJI RGCY 6 cut(s) 20, 152, 159, 190, 256, 662
CviKI_1 RGCY 6 cut(s) 20, 152, 159, 190, 256, 662
DdeI CTNAG 2 cut(s) 223, 252
Eam1104I CTCTTC 1 cut(s) 19
EarI CTCTTC 1 cut(s) 19
Ecl136II GAGCTC 1 cut(s) 159
Eco24I GRGCYC 1 cut(s) 161
Eco53kI GAGCTC 1 cut(s) 159
EcoICRI GAGCTC 1 cut(s) 159
EcoT22I ATGCAT 1 cut(s) 549
EcoT38I GRGCYC 1 cut(s) 161
Esp3I CGTCTC 1 cut(s) 79
FaeI CATG 2 cut(s) 188, 283
FaqI GGGAC 2 cut(s) 348, 579
FatI CATG 2 cut(s) 184, 279
FauI CCCGC 1 cut(s) 47
FauNDI CATATG 1 cut(s) 630
Fnu4HI GCNGC 6 cut(s) 21, 26, 112, 133, 153, 763
FokI GGATG 2 cut(s) 81, 659
FriOI GRGCYC 1 cut(s) 161
Fsp4HI GCNGC 6 cut(s) 21, 26, 112, 133, 153, 763
FspBI CTAG 1 cut(s) 726
GlaI GCGC 1 cut(s) 24
GluI GCNGC 6 cut(s) 21, 26, 112, 133, 153, 763
HhaI GCGC 1 cut(s) 25
Hin1II CATG 2 cut(s) 188, 283
Hin6I GCGC 1 cut(s) 23
HinP1I GCGC 1 cut(s) 23
HincII GTYRAC 1 cut(s) 277
HindII GTYRAC 1 cut(s) 277
HinfI GANTC 5 cut(s) 11, 97, 458, 469, 503
Hpy166II GTNNAC 2 cut(s) 277, 536
Hpy188I TCNGA 4 cut(s) 53, 84, 402, 553
Hpy8I GTNNAC 2 cut(s) 277, 536
HpyCH4III ACNGT 1 cut(s) 613
HpyF10VI GCNNNNNNNGC 9 cut(s) 138, 196, 228, 303, 354, 483, 674, 714, 768
HpyF3I CTNAG 2 cut(s) 223, 252
Hsp92II CATG 2 cut(s) 188, 283
HspAI GCGC 1 cut(s) 23
LmnI GCTCC 1 cut(s) 608
LpnPI CCDG 8 cut(s) 131, 142, 181, 197, 230, 492, 687, 799
Lsp1109I GCAGC 2 cut(s) 7, 98
LweI GCATC 4 cut(s) 59, 80, 558, 637
MaeI CTAG 1 cut(s) 726
MaeIII GTNAC 1 cut(s) 514
MboII GAAGA 6 cut(s) 6, 20, 304, 382, 467, 478
MhlI GDGCHC 3 cut(s) 106, 161, 618
MluCI AATT 5 cut(s) 338, 360, 385, 654, 699
MlyI GAGTC 3 cut(s) 5, 452, 497
MmeI TCCRAC 1 cut(s) 624
MnlI CCTC 1 cut(s) 685
Mph1103I ATGCAT 1 cut(s) 549
MroXI GAANNNNTTC 1 cut(s) 389
MseI TTAA 1 cut(s) 510
MslI CAYNNNNRTG 4 cut(s) 348, 447, 566, 668
Mva1269I GAATGC 1 cut(s) 547
MvnI CGCG 1 cut(s) 155
MwoI GCNNNNNNNGC 9 cut(s) 138, 196, 228, 303, 354, 483, 674, 714, 768
NdeI CATATG 1 cut(s) 630
NlaIII CATG 2 cut(s) 188, 283
NmuCI GTSAC 1 cut(s) 514
NsiI ATGCAT 1 cut(s) 549
OliI CACNNNNGTG 1 cut(s) 447
PctI GAATGC 1 cut(s) 547
PdmI GAANNNNTTC 1 cut(s) 389
PfeI GAWTC 2 cut(s) 97, 469
PkrI GCNGC 6 cut(s) 22, 27, 113, 134, 154, 764
PleI GAGTC 3 cut(s) 5, 452, 497
PpsI GAGTC 3 cut(s) 5, 452, 497
Psp124BI GAGCTC 1 cut(s) 161
RseI CAYNNNNRTG 4 cut(s) 348, 447, 566, 668
SacI GAGCTC 1 cut(s) 161
SaqAI TTAA 1 cut(s) 510
SatI GCNGC 6 cut(s) 21, 26, 112, 133, 153, 763
SchI GAGTC 3 cut(s) 5, 452, 497
SduI GDGCHC 3 cut(s) 106, 161, 618
SetI ASST 7 cut(s) 161, 192, 216, 249, 258, 696, 805
SfaNI GCATC 4 cut(s) 59, 80, 558, 637
SmiMI CAYNNNNRTG 4 cut(s) 348, 447, 566, 668
Sse9I AATT 5 cut(s) 338, 360, 385, 654, 699
SsiI CCGC 9 cut(s) 26, 40, 133, 153, 181, 270, 357, 677, 762
SspI AATATT 1 cut(s) 523
SspMI CTAG 1 cut(s) 726
SstI GAGCTC 1 cut(s) 161
TaaI ACNGT 1 cut(s) 613
TaqI TCGA 2 cut(s) 315, 618
TasI AATT 5 cut(s) 338, 360, 385, 654, 699
TauI GCSGC 4 cut(s) 28, 135, 155, 765
TfiI GAWTC 2 cut(s) 97, 469
Tru1I TTAA 1 cut(s) 510
Tru9I TTAA 1 cut(s) 510
TscAI CASTG 5 cut(s) 143, 429, 479, 618, 670
TseFI GTSAC 1 cut(s) 514
TseI GCWGC 2 cut(s) 20, 111
Tsp45I GTSAC 1 cut(s) 514
TspDTI ATGAA 5 cut(s) 332, 479, 542, 550, 765
TspGWI ACGGA 1 cut(s) 81
TspRI CASTG 5 cut(s) 143, 429, 479, 618, 670
XapI RAATTY 2 cut(s) 360, 385
XmnI GAANNNNTTC 1 cut(s) 389
XspI CTAG 1 cut(s) 726
Zsp2I ATGCAT 1 cut(s) 549
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.