Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr03

Orthogroup ID

OG0006576

Physical Location & Seq

Genomic Coordinates Reverse (-)

36765972 .. 36770812

Gene Structure 4841 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

MD03G1289500.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1542 bp

ATGAAGGCCACCGAAAAGTTTAGTGGCATGAGCGTGAAGCAATTGCGCGAAGAAGCCGCTCTTTGTGGAGTTTCGGCAACTGGGTCGAAAAAAGAATTGCTGGAGAGGCTTTCTAAACATTCTGATGGCATTCCTCTAGGTAAAGATTTTTGTTTTTCGAATTACAACTGGGTGTTTATTTTGTTATGCTTTGTGGCGATGCTAGCTAATGAGGAAGGAAATGGCAGCAAGGAGAAGATAGTAAACGCAACCAAAAAGGGTATGACGGTACTGGATCAGTGGCTACCGGATGACATAAAGGCAGATTATCATGGGGATGATATATACGATGCCATGTTAAACCAGGCCAATGTCGTTCTTAACAATAACAAGTTCTATCTGATACAAGTTCTTGAATCTGAAGTTGGTGGAAGTTTCATGATTTACTATAGATGCGGGAGAGTAGGTTTTAAGGGTCAGAACAAGCTAGCTCCTCACGCATCCTGCGAGAGCGCGATCAAGGAGTTTAAACAGAAATTCCGTGACAAAACAGGGAATGATTGGTCCAATCGAAAAATGTTCCAGCTTATCCCATCCTGCTATATGTGGATAGAAATGGATTACAATGAAAAGGAAGAGCAGTCAGCTGTCGAAGAAAAGAATGGCTCTGCTTTAGGACGTCAACCTTTGGAAACACAACTCGAGCCCTGCATTGCAAATTTTATATCTCTTATTTTCAACATTGACATGATGAAGCCGCATGTGATGGAAATAGATAACTTTGTGATTGATACTCCTCAGAAGTTGAAACACAAGCTAGAAATGGTTGAAGCACTAGGTGAAATTGAGGTTGCAACGAAATTGTTGAAGGATGACACAGGAAGGCAGGGAGATCCCTTGTATTCCTGTTATCGACGCCTTCATTGTGAGCTGACACCGGTTGGTGCTGATTCTCATGAATTTGATATGATTACAAAATATATGCGTAATACTCATGCAAAAACACATTCAACTTACACTGTTGATATTGTTCAAATATTCCGCACATCTAAAGAGGGTGAAGTTGAACGCTTCAGAAAGTTTTCTAGTACGAAAAATAGAATGCTTTTATGGCATGGTTCTCGTCTTACAAATTGGGTTGGTATATTATCTCAAGGCTTGCTCATAACTCCACCTGAAGCCCCAGTGACTGGTCACATGTTCGGGAAAGGAGTTTACTTTGCAGACATGTTCTCCAAAAGCGCAAAGTATTGCCATGGCTGTACATCCGGGGTGCTGCTTCTATGTGAGGTTGCGCTGGGTGACATGGCTGAGCTTTTAACCGCTAAGTACGATGCTGACAAGTTACCTGTAGGAAAACTAAGCACTAAAGGAGTTGGGGGAACTGAACCAGATTTTTCAGAAGCTCAGTTACTAGATGACGGTGTCGTGGTTCCCCTAGGAAAGCAGAAAGAGAATACAAGCCACGAGGGTTCATTACGGTACAATGAGTACATAGTTTACAATGTGGAACAGATCAGGATGCGCTACGTTGTTCAAGTTAATTTCAATTTCAAGATATGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000726 GO:0003674 GO:0003824 GO:0003909 GO:0003910 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005737 GO:0006139 GO:0006259 GO:0006260 GO:0006261 GO:0006266 GO:0006271 GO:0006273 GO:0006281 GO:0006302 GO:0006303 GO:0006725 GO:0006807 GO:0006950 GO:0006974 GO:0008150 GO:0008152 GO:0009058 GO:0009059 GO:0009987 GO:0016070 GO:0016874 GO:0016886 GO:0022616 GO:0033554 GO:0034641 GO:0034645 GO:0043170 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044237 GO:0044238 GO:0044249 GO:0044260 GO:0044424 GO:0044464 GO:0046483 GO:0050896 GO:0051103 GO:0051716 GO:0071704 GO:0090304 GO:0140097 GO:1901360 GO:1901576

KEGG Pathways

Metabolic & Signaling

ko03410 ko04210 ko04212 ko04214 ko04217 map03410 map04210 map04212 map04214 map04217

Pfam Domains

Protein Families

PARP PARP_reg SAP WGR

Protein Analysis

514

Amino Acids

58.06

Weight (kDa)

6.27

Isoelectric Point (pI)

26.88

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
SAP	PF02037	7 - 40	3.7e-07	SAP domain
WGR	PF05406	109 - 187	1.2e-20	WGR domain
PARP_reg	PF02877	253 - 297	2.5e-07	Poly(ADP-ribose) polymerase, regulatory domain
PARP	PF00644	311 - 509	3e-77	Poly(ADP-ribose) polymerase catalytic domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0006576)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT4G02390 AT4G02390 AT4G02390
fragaria_vesca	FvH4_2g25840 FvH4_2g25840 FvH4_2g25840
malus_domestica	MD03G1289400.v1.1 MD03G1289500.v1.1 MD03G1289600.v1.1
prunus_persica	Prupe.8G262600_v2.0.a1 Prupe.8G262600_v2.0.a1 Prupe.8G262600_v2.0.a1 Prupe.8G262600_v2.0.a1 Prupe.8G262600_v2.0.a1
pyrus_communis	pycom03g22920
rosa_chinensis	RchiOBHm_Chr6g0294171
rosa_laevigata	RLG00000011893
rosa_multiflora	Rmu_sc0001521.1_g000044
rosa_roxburghii	Rroxscaffold_7G00173580
rosa_rugosa	Rorug06G0242000 Rorug06G0242100
rosa_samantha	Rh6AG354300 Rh6BG361200 Rh6CG368500 Rh6DG355300
rosa_wichuraiana	Rw6G030880

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AatII	GACGTC	1 cut(s)	661
AccB7I	CCANNNNNTGG	1 cut(s)	1169
AccBSI	CCGCTC	1 cut(s)	59
AccII	CGCG	2 cut(s)	48, 494
AciI	CCGC	5 cut(s)	57, 435, 737, 1021, 1302
AclWI	GGATC	2 cut(s)	282, 866
AcsI	RAATTY	3 cut(s)	515, 697, 938
AcuI	CTGAAG	3 cut(s)	420, 1036, 1176
AcyI	GRCGYC	2 cut(s)	658, 895
AdeI	CACNNNGTG	1 cut(s)	818
AfaI	GTAC	6 cut(s)	270, 1069, 1243, 1310, 1463, 1472
AfiI	CCNNNNNNNGG	1 cut(s)	1169
AflIII	ACRYGT	2 cut(s)	1176, 1206
AgeI	ACCGGT	1 cut(s)	916
AjnI	CCWGG	1 cut(s)	342
AjuI	GAANNNNNNNTTGG	2 cut(s)	387, 419
AluBI	AGCT	9 cut(s)	206, 466, 470, 565, 626, 796, 910, 1294, 1385
AluI	AGCT	9 cut(s)	206, 466, 470, 565, 626, 796, 910, 1294, 1385
AlwI	GGATC	2 cut(s)	282, 866
AlwNI	CAGNNNCTG	1 cut(s)	1169
Ama87I	CYCGRG	1 cut(s)	680
AoxI	GGCC	2 cut(s)	6, 345
ApeKI	GCWGC	2 cut(s)	225, 1255
ApoI	RAATTY	3 cut(s)	515, 697, 938
AsiGI	ACCGGT	1 cut(s)	916
Asp700I	GAANNNNTTC	1 cut(s)	1060
AspA2I	CCTAGG	1 cut(s)	1417
AspLEI	GCGC	5 cut(s)	48, 494, 1223, 1276, 1506
AspS9I	GGNCC	1 cut(s)	543
AsuC2I	CCSGG	1 cut(s)	1249
AsuHPI	GGTGA	3 cut(s)	830, 1049, 1292
AsuII	TTCGAA	1 cut(s)	158
AsuNHI	GCTAGC	2 cut(s)	202, 466
AvaI	CYCGRG	1 cut(s)	680
AvaII	GGWCC	1 cut(s)	543
AvrII	CCTAGG	1 cut(s)	1417
BanII	GRGCYC	1 cut(s)	687
BarI	GAAGNNNNNNTAC	2 cut(s)	1374, 1406
BauI	CACGAG	1 cut(s)	1445
BbvI	GCAGC	2 cut(s)	237, 1242
BccI	CCATC	3 cut(s)	119, 580, 739
BcgI	CGANNNNNNTGC	4 cut(s)	66, 100, 1082, 1116
BciT130I	CCWGG	1 cut(s)	344
BcnI	CCSGG	1 cut(s)	1249
BfaI	CTAG	8 cut(s)	137, 203, 467, 797, 815, 1065, 1394, 1418
BfmI	CTRYAG	2 cut(s)	427, 1329
BisI	GCNGC	4 cut(s)	57, 226, 737, 1256
BlnI	CCTAGG	1 cut(s)	1417
BlpI	GCTNAGC	1 cut(s)	1290
BlsI	GCNGC	4 cut(s)	58, 227, 738, 1257
Bme1390I	CCNGG	2 cut(s)	344, 1249
Bme18I	GGWCC	1 cut(s)	543
BmeT110I	CYCGRG	1 cut(s)	680
BmgT120I	GGNCC	1 cut(s)	543
BmiI	GGNNCC	1 cut(s)	1413
BmrFI	CCNGG	2 cut(s)	344, 1249
BmrI	ACTGGG	3 cut(s)	90, 178, 1157
BmsI	GCATC	6 cut(s)	189, 319, 422, 488, 1303, 1491
BmtI	GCTAGC	2 cut(s)	206, 470
BmuI	ACTGGG	3 cut(s)	90, 178, 1157
BpmI	CTGGAG	1 cut(s)	122
Bpu1102I	GCTNAGC	1 cut(s)	1290
Bpu14I	TTCGAA	1 cut(s)	158
BpuEI	CTTGAG	1 cut(s)	1116
BpuMI	CCSGG	1 cut(s)	1249
BsaHI	GRCGYC	2 cut(s)	658, 895
BsaJI	CCNNGG	3 cut(s)	1234, 1248, 1417
BsaWI	WCCGGW	2 cut(s)	286, 916
BsaXI	ACNNNNNCTCC	4 cut(s)	430, 460, 1196, 1226
Bsc4I	CCNNNNNNNGG	1 cut(s)	1169
Bse118I	RCCGGY	1 cut(s)	916
Bse1I	ACTGG	5 cut(s)	85, 173, 276, 1163, 1174
Bse3DI	GCAATG	1 cut(s)	690
BseBI	CCWGG	1 cut(s)	344
BseDI	CCNNGG	3 cut(s)	1234, 1248, 1417
BseGI	GGATG	7 cut(s)	295, 322, 479, 572, 856, 1244, 1506
BseLI	CCNNNNNNNGG	1 cut(s)	1169
BseMI	GCAATG	1 cut(s)	690
BseMII	CTCAG	3 cut(s)	791, 1281, 1400
BseNI	ACTGG	5 cut(s)	85, 173, 276, 1163, 1174
BseRI	GAGGAG	2 cut(s)	462, 765
BseXI	GCAGC	2 cut(s)	237, 1242
BseYI	CCCAGC	1 cut(s)	1276
Bsh1236I	CGCG	2 cut(s)	48, 494
BshFI	GGCC	2 cut(s)	8, 347
BshTI	ACCGGT	1 cut(s)	916
BsiHKCI	CYCGRG	1 cut(s)	680
BsiSI	CCGG	3 cut(s)	287, 917, 1248
BslI	CCNNNNNNNGG	1 cut(s)	1169
BsmI	GAATGC	2 cut(s)	129, 1086
BsnI	GGCC	2 cut(s)	8, 347
BsoBI	CYCGRG	1 cut(s)	680
Bsp119I	TTCGAA	1 cut(s)	158
Bsp1286I	GDGCHC	1 cut(s)	687
Bsp1407I	TGTACA	1 cut(s)	1241
Bsp143I	GATC	4 cut(s)	274, 495, 871, 1494
Bsp1720I	GCTNAGC	1 cut(s)	1290
Bsp19I	CCATGG	1 cut(s)	1234
BspACI	CCGC	5 cut(s)	57, 435, 737, 1021, 1302
BspANI	GGCC	2 cut(s)	8, 347
BspCNI	CTCAG	3 cut(s)	790, 1282, 1399
BspFNI	CGCG	2 cut(s)	48, 494
BspHI	TCATGA	2 cut(s)	417, 934
BspLI	GGNNCC	1 cut(s)	1413
BspOI	GCTAGC	2 cut(s)	206, 470
BspPI	GGATC	2 cut(s)	282, 866
BspQI	GCTCTTC	1 cut(s)	609
BspT104I	TTCGAA	1 cut(s)	158
BsrBI	CCGCTC	1 cut(s)	59
BsrDI	GCAATG	1 cut(s)	690
BsrFI	RCCGGY	1 cut(s)	916
BsrGI	TGTACA	1 cut(s)	1241
BsrI	ACTGG	5 cut(s)	85, 173, 276, 1163, 1174
BssAI	RCCGGY	1 cut(s)	916
BssECI	CCNNGG	3 cut(s)	1234, 1248, 1417
BssMI	GATC	4 cut(s)	274, 495, 871, 1494
BssNI	GRCGYC	2 cut(s)	658, 895
BssSI	CACGAG	1 cut(s)	1445
BssT1I	CCWWGG	2 cut(s)	1234, 1417
Bst2BI	CACGAG	1 cut(s)	1445
Bst2UI	CCWGG	1 cut(s)	344
Bst4CI	ACNGT	4 cut(s)	268, 1000, 1403, 1461
Bst6I	CTCTTC	1 cut(s)	609
BstACI	GRCGYC	2 cut(s)	658, 895
BstAUI	TGTACA	1 cut(s)	1241
BstBI	TTCGAA	1 cut(s)	158
BstC8I	GCNNGC	3 cut(s)	204, 468, 1139
BstDEI	CTNAG	5 cut(s)	777, 1290, 1305, 1340, 1386
BstDSI	CCRYGG	1 cut(s)	1234
BstF5I	GGATG	7 cut(s)	295, 322, 479, 572, 856, 1244, 1506
BstFNI	CGCG	2 cut(s)	48, 494
BstHHI	GCGC	5 cut(s)	48, 494, 1223, 1276, 1506
BstKTI	GATC	4 cut(s)	277, 498, 874, 1497
BstMBI	GATC	4 cut(s)	274, 495, 871, 1494
BstMWI	GCNNNNNNNGC	4 cut(s)	106, 203, 476, 1090
BstNI	CCWGG	1 cut(s)	344
BstNSI	RCATGY	3 cut(s)	743, 1180, 1210
BstSCI	CCNGG	2 cut(s)	342, 1247
BstSFI	CTRYAG	2 cut(s)	427, 1329
BstUI	CGCG	2 cut(s)	48, 494
BstV1I	GCAGC	2 cut(s)	237, 1242
BstX2I	RGATCY	1 cut(s)	871
BstYI	RGATCY	1 cut(s)	871
BsuRI	GGCC	2 cut(s)	8, 347
BtgI	CCRYGG	1 cut(s)	1234
BtgZI	GCGATG	1 cut(s)	212
BtsCI	GGATG	7 cut(s)	295, 322, 479, 572, 856, 1244, 1506
BtsIMutI	CAGTG	3 cut(s)	284, 996, 1170
Cac8I	GCNNGC	3 cut(s)	204, 468, 1139
CaiI	CAGNNNCTG	1 cut(s)	1169
CciI	TCATGA	2 cut(s)	417, 934
CfoI	GCGC	5 cut(s)	48, 494, 1223, 1276, 1506
Cfr10I	RCCGGY	1 cut(s)	916
Cfr13I	GGNCC	1 cut(s)	543
CseI	GACGC	1 cut(s)	903
Csp6I	GTAC	6 cut(s)	269, 1068, 1242, 1309, 1462, 1471
CspAI	ACCGGT	1 cut(s)	916
CviQI	GTAC	6 cut(s)	269, 1068, 1242, 1309, 1462, 1471
DdeI	CTNAG	5 cut(s)	777, 1290, 1305, 1340, 1386
DpnI	GATC	4 cut(s)	276, 497, 873, 1496
DpnII	GATC	4 cut(s)	274, 495, 871, 1494
DraI	TTTAAA	1 cut(s)	508
DraIII	CACNNNGTG	1 cut(s)	818
Eam1104I	CTCTTC	1 cut(s)	609
EarI	CTCTTC	1 cut(s)	609
Eco130I	CCWWGG	2 cut(s)	1234, 1417
Eco24I	GRGCYC	1 cut(s)	687
Eco47I	GGWCC	1 cut(s)	543
Eco57I	CTGAAG	3 cut(s)	420, 1036, 1176
Eco88I	CYCGRG	1 cut(s)	680
EcoRII	CCWGG	1 cut(s)	342
EcoT14I	CCWWGG	2 cut(s)	1234, 1417
EcoT38I	GRGCYC	1 cut(s)	687
ErhI	CCWWGG	2 cut(s)	1234, 1417
FalI	AAGNNNNNCTT	2 cut(s)	45, 77
FauI	CCCGC	1 cut(s)	428
Fnu4HI	GCNGC	4 cut(s)	57, 226, 737, 1256
FokI	GGATG	7 cut(s)	302, 329, 466, 559, 863, 1231, 1513
FriOI	GRGCYC	1 cut(s)	687
Fsp4HI	GCNGC	4 cut(s)	57, 226, 737, 1256
FspBI	CTAG	8 cut(s)	137, 203, 467, 797, 815, 1065, 1394, 1418
GlaI	GCGC	5 cut(s)	47, 493, 1222, 1275, 1505
GluI	GCNGC	4 cut(s)	57, 226, 737, 1256
GsaI	CCCAGC	1 cut(s)	1280
GsuI	CTGGAG	1 cut(s)	122
HaeIII	GGCC	2 cut(s)	8, 347
HapII	CCGG	3 cut(s)	287, 917, 1248
HgaI	GACGC	1 cut(s)	903
HhaI	GCGC	5 cut(s)	48, 494, 1223, 1276, 1506
Hin1I	GRCGYC	2 cut(s)	658, 895
Hin6I	GCGC	5 cut(s)	46, 492, 1221, 1274, 1504
HinP1I	GCGC	5 cut(s)	46, 492, 1221, 1274, 1504
HincII	GTYRAC	1 cut(s)	662
HindII	GTYRAC	1 cut(s)	662
HinfI	GANTC	2 cut(s)	395, 929
HpaII	CCGG	3 cut(s)	287, 917, 1248
HphI	GGTGA	3 cut(s)	830, 1049, 1292
Hpy166II	GTNNAC	4 cut(s)	244, 662, 1195, 1480
Hpy188I	TCNGA	7 cut(s)	124, 381, 400, 459, 780, 1055, 1381
Hpy188III	TCNNGA	6 cut(s)	392, 418, 935, 1183, 1498, 1534
Hpy8I	GTNNAC	4 cut(s)	244, 662, 1195, 1480
Hpy99I	CGWCG	1 cut(s)	897
HpyAV	CCTTC	4 cut(s)	209, 841, 855, 908
HpyCH4III	ACNGT	4 cut(s)	268, 1000, 1403, 1461
HpyCH4IV	ACGT	2 cut(s)	658, 1509
HpyCH4V	TGCA	5 cut(s)	690, 695, 833, 977, 1202
HpyF10VI	GCNNNNNNNGC	4 cut(s)	106, 203, 476, 1090
HpyF3I	CTNAG	5 cut(s)	777, 1290, 1305, 1340, 1386
HpySE526I	ACGT	2 cut(s)	658, 1509
Hsp92I	GRCGYC	2 cut(s)	658, 895
HspAI	GCGC	5 cut(s)	46, 492, 1221, 1274, 1504
Kzo9I	GATC	4 cut(s)	274, 495, 871, 1494
LguI	GCTCTTC	1 cut(s)	609
LmnI	GCTCC	1 cut(s)	475
Lsp1109I	GCAGC	2 cut(s)	237, 1242
LweI	GCATC	6 cut(s)	189, 319, 422, 488, 1303, 1491
MaeI	CTAG	8 cut(s)	137, 203, 467, 797, 815, 1065, 1394, 1418
MaeII	ACGT	2 cut(s)	658, 1509
MaeIII	GTNAC	6 cut(s)	521, 1165, 1172, 1280, 1323, 1389
MalI	GATC	4 cut(s)	276, 497, 873, 1496
MbiI	CCGCTC	1 cut(s)	59
MboI	GATC	4 cut(s)	274, 495, 871, 1494
MboII	GAAGA	4 cut(s)	62, 247, 626, 644
MfeI	CAATTG	1 cut(s)	41
MflI	RGATCY	1 cut(s)	871
MhlI	GDGCHC	1 cut(s)	687
MnlI	CCTC	9 cut(s)	99, 144, 205, 483, 786, 820, 1027, 1261, 1441
MroXI	GAANNNNTTC	1 cut(s)	1060
MseI	TTAA	6 cut(s)	338, 360, 450, 507, 1298, 1521
MslI	CAYNNNNRTG	4 cut(s)	32, 123, 315, 725
MspA1I	CMGCKG	1 cut(s)	626
MspI	CCGG	3 cut(s)	287, 917, 1248
MspR9I	CCNGG	2 cut(s)	344, 1249
MssI	GTTTAAAC	1 cut(s)	508
MunI	CAATTG	1 cut(s)	41
Mva1269I	GAATGC	2 cut(s)	129, 1086
MvaI	CCWGG	1 cut(s)	344
MvnI	CGCG	2 cut(s)	48, 494
MwoI	GCNNNNNNNGC	4 cut(s)	106, 203, 476, 1090
NciI	CCSGG	1 cut(s)	1249
NcoI	CCATGG	1 cut(s)	1234
NdeII	GATC	4 cut(s)	274, 495, 871, 1494
NheI	GCTAGC	2 cut(s)	202, 466
NlaIV	GGNNCC	1 cut(s)	1413
NmuCI	GTSAC	4 cut(s)	521, 1165, 1172, 1280
NspI	RCATGY	3 cut(s)	743, 1180, 1210
NspV	TTCGAA	1 cut(s)	158
PaeR7I	CTCGAG	1 cut(s)	680
PagI	TCATGA	2 cut(s)	417, 934
PciI	ACATGT	2 cut(s)	1176, 1206
PciSI	GCTCTTC	1 cut(s)	609
PcsI	WCGNNNNNNNCGW	1 cut(s)	483
PctI	GAATGC	2 cut(s)	129, 1086
PdmI	GAANNNNTTC	1 cut(s)	1060
PfeI	GAWTC	2 cut(s)	395, 929
PflFI	GACNNNGTC	1 cut(s)	1403
PflMI	CCANNNNNTGG	1 cut(s)	1169
PinAI	ACCGGT	1 cut(s)	916
PkrI	GCNGC	4 cut(s)	58, 227, 738, 1257
PmeI	GTTTAAAC	1 cut(s)	508
PscI	ACATGT	2 cut(s)	1176, 1206
Psp6I	CCWGG	1 cut(s)	342
PspFI	CCCAGC	1 cut(s)	1276
PspGI	CCWGG	1 cut(s)	342
PspN4I	GGNNCC	1 cut(s)	1413
PspPI	GGNCC	1 cut(s)	543
PspXI	VCTCGAGB	1 cut(s)	680
PstNI	CAGNNNCTG	1 cut(s)	1169
PsuI	RGATCY	1 cut(s)	871
PsyI	GACNNNGTC	1 cut(s)	1403
PvuII	CAGCTG	1 cut(s)	626
RsaI	GTAC	6 cut(s)	270, 1069, 1243, 1310, 1463, 1472
RsaNI	GTAC	6 cut(s)	269, 1068, 1242, 1309, 1462, 1471
RseI	CAYNNNNRTG	4 cut(s)	32, 123, 315, 725
SapI	GCTCTTC	1 cut(s)	609
SaqAI	TTAA	6 cut(s)	338, 360, 450, 507, 1298, 1521
SatI	GCNGC	4 cut(s)	57, 226, 737, 1256
Sau3AI	GATC	4 cut(s)	274, 495, 871, 1494
Sau96I	GGNCC	1 cut(s)	543
ScrFI	CCNGG	2 cut(s)	344, 1249
SduI	GDGCHC	1 cut(s)	687
SfaNI	GCATC	6 cut(s)	189, 319, 422, 488, 1303, 1491
SfcI	CTRYAG	2 cut(s)	427, 1329
Sfr274I	CTCGAG	1 cut(s)	680
SfuI	TTCGAA	1 cut(s)	158
SinI	GGWCC	1 cut(s)	543
SlaI	CTCGAG	1 cut(s)	680
SmiMI	CAYNNNNRTG	4 cut(s)	32, 123, 315, 725
SmlI	CTYRAG	2 cut(s)	680, 1131
SmoI	CTYRAG	2 cut(s)	680, 1131
SsiI	CCGC	5 cut(s)	57, 435, 737, 1021, 1302
SspI	AATATT	1 cut(s)	1017
SspMI	CTAG	8 cut(s)	137, 203, 467, 797, 815, 1065, 1394, 1418
StyD4I	CCNGG	2 cut(s)	342, 1247
StyI	CCWWGG	2 cut(s)	1234, 1417
TaaI	ACNGT	4 cut(s)	268, 1000, 1403, 1461
TaiI	ACGT	2 cut(s)	661, 1512
TaqI	TCGA	6 cut(s)	86, 158, 550, 630, 681, 892
TatI	WGTACW	2 cut(s)	1241, 1470
TauI	GCSGC	2 cut(s)	59, 739
TfiI	GAWTC	2 cut(s)	395, 929
Tru1I	TTAA	6 cut(s)	338, 360, 450, 507, 1298, 1521
Tru9I	TTAA	6 cut(s)	338, 360, 450, 507, 1298, 1521
TscAI	CASTG	3 cut(s)	284, 1003, 1170
TseFI	GTSAC	4 cut(s)	521, 1165, 1172, 1280
TseI	GCWGC	2 cut(s)	225, 1255
Tsp45I	GTSAC	4 cut(s)	521, 1165, 1172, 1280
TspDTI	ATGAA	7 cut(s)	17, 406, 621, 746, 890, 951, 1443
TspGWI	ACGGA	1 cut(s)	509
TspRI	CASTG	3 cut(s)	284, 1003, 1170
Tth111I	GACNNNGTC	1 cut(s)	1403
Van91I	CCANNNNNTGG	1 cut(s)	1169
VpaK11BI	GGWCC	1 cut(s)	543
XapI	RAATTY	3 cut(s)	515, 697, 938
XceI	RCATGY	3 cut(s)	743, 1180, 1210
XhoI	CTCGAG	1 cut(s)	680
XmaJI	CCTAGG	1 cut(s)	1417
XmnI	GAANNNNTTC	1 cut(s)	1060
XspI	CTAG	8 cut(s)	137, 203, 467, 797, 815, 1065, 1394, 1418
ZraI	GACGTC	1 cut(s)	659

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.