Basic Information

Type: gene

Biological Identity

Species

rosa_samantha

Chromosome

Chr6B

Orthogroup ID

OG0006576

Physical Location & Seq

Genomic Coordinates Reverse (-)

59148552 .. 59154226

Gene Structure 5675 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

Rh6BG361200.1

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1425 bp

ATGGCGAACAAGCTCAAAGTAGAACAACTCCGAGACCAATTGGCCCAGCGTGGTCTCAGCACCGCCGGAACCAAGCCCGTCCTGGTTTCGAGGCTCGAGTCCGCTCTTCACAGCCAAACCGCAGCCTCAACCGACGGCTCAGCCACTAAAAAGAGAGGAAGGAACTCCAATGAAGATGAGGATTCAACGGCGTCCAAGAAAATCAAGGCAACTGAGAAACTCCGGAATATGAATATCAAGCAACTCCGCGAGGAAGCAACTCTTCGCGGAATTCCGGCTACTGGTTCCAAGAAGGAGGTGCTGGAGAGGCTCTGTCAAGATTCCGATAAGGATTGCATTGACATTGATGCGAGTAGAGGAGTAGTGAAGGAAGAAGGGAATGACAGCAAGGAGAAGATGGTTACAGCTACCAAAAAAGGTGCGGCTGTGTTGGATCAGTGGCTGCCGGATAATATAAAGACACATTATCATGTTTTGCAACTGGGTGATGATGTGTATGATGCCATGTTGAACCAGACCAATGTTGGCCATAACAATAACAAGTTCTATCTACTTCAAGTTCTTGAATCTGATGTTGGTGGTAGTTTCATGGTTTACTATAGATGGGGGAGAGTAGGTGTCAAGGGTCAAAACAAGTTACTAGGCCCTTACACATCCCGAGACAGGGCAATTGAGGAATTTGAAAAGAAATTCTCTGAAAAAACCAGGAATTATTGGTCCGAACGAAAAACATTCCAGTCATTCCCAAAGTGCTATGTGTGGTTAGAAATGGATTACAGCGAAAAGGAAGGCCAATCAGCTGTTGCAGAAAAGAATGGCTCAGCTTTAGTACCTCAACCTTTGGAAACAAAACTTGATCCCCGTATCGCAAAGCTCGTATCTCTTATCTGCAACATTGCCATGATGAAGCAGTATATGATGGAAATAGGATACAATGCTGATAAATTGCCACTGGGGAAGCTGAGCAAATCAACAATTCTAAAGGGTTACAGTATACTGAAGAGTATTGCTGATGTGATTGGCCAGTCTAATAAGAAAACGCTTGAACAATTAAGTGGAGAGTTCTACACTGTCATTCCTCATGATTTTGGATTTAAAAAGATGAGCAACTTTGTGATTGATACTCCTCAGAAGTTGAAGCAAAAGTTGGAAATGGTTGAAGCACTAGGTGAAATTGAAGTTGCAACAAAATTGTTGAATGATGATAACGGGACACAGGAAGATCCCTTGTATTCTAATTACCAGAGCCTCTGCTGTGAGCTGACACCGCTTGGTGCCGATTCAGATGAATTTGATATGGTTTCAAAGTATTTGCATAACACCCATGCAAAAACGCATTCAAATTATACCGTTGATATCGTTCAGATATTCCGTGCATCAAGAGAGGGTGAAGCTGAACGTTATAGAAAGGTTAGGCTGGGATGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000726 GO:0003674 GO:0003824 GO:0003909 GO:0003910 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005737 GO:0006139 GO:0006259 GO:0006260 GO:0006261 GO:0006266 GO:0006271 GO:0006273 GO:0006281 GO:0006302 GO:0006303 GO:0006725 GO:0006807 GO:0006950 GO:0006974 GO:0008150 GO:0008152 GO:0009058 GO:0009059 GO:0009987 GO:0016070 GO:0016874 GO:0016886 GO:0022616 GO:0033554 GO:0034641 GO:0034645 GO:0043170 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044237 GO:0044238 GO:0044249 GO:0044260 GO:0044424 GO:0044464 GO:0046483 GO:0050896 GO:0051103 GO:0051716 GO:0071704 GO:0090304 GO:0140097 GO:1901360 GO:1901576

KEGG Pathways

Metabolic & Signaling

ko03410 ko04210 ko04212 ko04214 ko04217 map03410 map04210 map04212 map04214 map04217

Pfam Domains

Protein Families

PARP PARP_reg SAP WGR

Protein Analysis

474

Amino Acids

53.45

Weight (kDa)

8.44

Isoelectric Point (pI)

37.48

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
SAP	PF02037	2 - 36	1.1e-11	SAP domain
WGR	PF05406	166 - 245	1e-24	WGR domain
PARP_reg	PF02877	284 - 414	2.9e-42	Poly(ADP-ribose) polymerase, regulatory domain
PARP	PF00644	428 - 471	1.2e-08	Poly(ADP-ribose) polymerase catalytic domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0006576)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT4G02390 AT4G02390 AT4G02390
fragaria_vesca	FvH4_2g25840 FvH4_2g25840 FvH4_2g25840
malus_domestica	MD03G1289400.v1.1 MD03G1289500.v1.1 MD03G1289600.v1.1
prunus_persica	Prupe.8G262600_v2.0.a1 Prupe.8G262600_v2.0.a1 Prupe.8G262600_v2.0.a1 Prupe.8G262600_v2.0.a1 Prupe.8G262600_v2.0.a1
pyrus_communis	pycom03g22920
rosa_chinensis	RchiOBHm_Chr6g0294171
rosa_laevigata	RLG00000011893
rosa_multiflora	Rmu_sc0001521.1_g000044
rosa_roxburghii	Rroxscaffold_7G00173580
rosa_rugosa	Rorug06G0242000 Rorug06G0242100
rosa_samantha	Rh6AG354300 Rh6BG361200 Rh6CG368500 Rh6DG355300
rosa_wichuraiana	Rw6G030880

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AccB1I	GGYRCC	1 cut(s)	1274
AccBSI	CCGCTC	1 cut(s)	104
AccI	GTMKAC	1 cut(s)	994
AccII	CGCG	2 cut(s)	249, 267
AccIII	TCCGGA	1 cut(s)	222
AciI	CCGC	7 cut(s)	63, 102, 120, 247, 267, 422, 1268
AclI	AACGTT	1 cut(s)	1399
AclWI	GGATC	3 cut(s)	441, 851, 1217
AcoI	YGGCCR	2 cut(s)	526, 1021
AcsI	RAATTY	4 cut(s)	270, 677, 689, 1289
AcuI	CTGAAG	1 cut(s)	1019
AcyI	GRCGYC	1 cut(s)	191
AdeI	CACNNNGTG	1 cut(s)	1169
AfaI	GTAC	1 cut(s)	831
AfiI	CCNNNNNNNGG	3 cut(s)	281, 663, 664
AjnI	CCWGG	2 cut(s)	81, 704
AjuI	GAANNNNNNNTTGG	4 cut(s)	558, 590, 1130, 1162
AluBI	AGCT	8 cut(s)	13, 407, 800, 824, 874, 961, 1261, 1394
AluI	AGCT	8 cut(s)	13, 407, 800, 824, 874, 961, 1261, 1394
Alw26I	GTCTC	3 cut(s)	27, 59, 654
AlwI	GGATC	3 cut(s)	441, 851, 1217
AlwNI	CAGNNNCTG	1 cut(s)	442
Ama87I	CYCGRG	2 cut(s)	95, 657
Aor13HI	TCCGGA	1 cut(s)	222
AoxI	GGCC	5 cut(s)	42, 526, 643, 790, 1021
ApeKI	GCWGC	2 cut(s)	122, 442
ApoI	RAATTY	4 cut(s)	270, 677, 689, 1289
AspS9I	GGNCC	3 cut(s)	43, 644, 717
AsuHPI	GGTGA	3 cut(s)	497, 1181, 1400
AvaI	CYCGRG	2 cut(s)	95, 657
AvaII	GGWCC	1 cut(s)	717
BalI	TGGCCA	2 cut(s)	528, 1023
BanI	GGYRCC	1 cut(s)	1274
BbvI	GCAGC	2 cut(s)	134, 429
BccI	CCATC	3 cut(s)	391, 597, 913
BceAI	ACGGC	2 cut(s)	151, 204
BciT130I	CCWGG	2 cut(s)	83, 706
BciVI	GTATCC	1 cut(s)	923
BcoDI	GTCTC	3 cut(s)	27, 59, 654
BfaI	CTAG	2 cut(s)	641, 1166
BfmI	CTRYAG	1 cut(s)	598
BfuI	GTATCC	1 cut(s)	923
BisI	GCNGC	3 cut(s)	123, 423, 443
BlpI	GCTNAGC	3 cut(s)	139, 820, 962
BlsI	GCNGC	3 cut(s)	124, 424, 444
Bme1390I	CCNGG	2 cut(s)	83, 706
Bme18I	GGWCC	1 cut(s)	717
BmeT110I	CYCGRG	2 cut(s)	95, 657
BmgT120I	GGNCC	3 cut(s)	43, 644, 717
BmiI	GGNNCC	3 cut(s)	70, 286, 1276
BmrFI	CCNGG	2 cut(s)	83, 706
BmrI	ACTGGG	2 cut(s)	491, 962
BmsI	GCATC	3 cut(s)	337, 490, 1385
BmuI	ACTGGG	2 cut(s)	491, 962
BpmI	CTGGAG	1 cut(s)	323
Bpu1102I	GCTNAGC	3 cut(s)	139, 820, 962
BsaHI	GRCGYC	1 cut(s)	191
BsaI	GGTCTC	2 cut(s)	27, 59
BsaWI	WCCGGW	1 cut(s)	222
BsaXI	ACNNNNNCTCC	2 cut(s)	601, 631
Bsc4I	CCNNNNNNNGG	3 cut(s)	281, 663, 664
Bse1I	ACTGG	5 cut(s)	286, 486, 736, 957, 1024
Bse3DI	GCAATG	1 cut(s)	894
BseAI	TCCGGA	1 cut(s)	222
BseBI	CCWGG	2 cut(s)	83, 706
BseGI	GGATG	1 cut(s)	653
BseLI	CCNNNNNNNGG	3 cut(s)	281, 663, 664
BseMI	GCAATG	1 cut(s)	894
BseMII	CTCAG	6 cut(s)	70, 153, 204, 834, 953, 1142
BseNI	ACTGG	5 cut(s)	286, 486, 736, 957, 1024
BseRI	GAGGAG	2 cut(s)	372, 1116
BseXI	GCAGC	2 cut(s)	134, 429
BseYI	CCCAGC	2 cut(s)	45, 1417
Bsh1236I	CGCG	2 cut(s)	249, 267
BshFI	GGCC	5 cut(s)	44, 528, 645, 792, 1023
BshNI	GGYRCC	1 cut(s)	1274
BsiHKCI	CYCGRG	2 cut(s)	95, 657
BsiSI	CCGG	4 cut(s)	66, 223, 275, 446
BslFI	GGGAC	1 cut(s)	1225
BslI	CCNNNNNNNGG	3 cut(s)	281, 663, 664
BsmAI	GTCTC	3 cut(s)	27, 59, 654
BsmFI	GGGAC	1 cut(s)	1225
BsmI	GAATGC	1 cut(s)	1336
BsnI	GGCC	5 cut(s)	44, 528, 645, 792, 1023
Bso31I	GGTCTC	2 cut(s)	27, 59
BsoBI	CYCGRG	2 cut(s)	95, 657
Bsp13I	TCCGGA	1 cut(s)	222
Bsp143I	GATC	3 cut(s)	433, 856, 1222
Bsp1720I	GCTNAGC	3 cut(s)	139, 820, 962
BspACI	CCGC	7 cut(s)	63, 102, 120, 247, 267, 422, 1268
BspANI	GGCC	5 cut(s)	44, 528, 645, 792, 1023
BspCNI	CTCAG	6 cut(s)	69, 152, 205, 833, 954, 1141
BspEI	TCCGGA	1 cut(s)	222
BspFNI	CGCG	2 cut(s)	249, 267
BspHI	TCATGA	1 cut(s)	1081
BspLI	GGNNCC	3 cut(s)	70, 286, 1276
BspPI	GGATC	3 cut(s)	441, 851, 1217
BspQI	GCTCTTC	1 cut(s)	111
BspT107I	GGYRCC	1 cut(s)	1274
BspTNI	GGTCTC	2 cut(s)	27, 59
BsrBI	CCGCTC	1 cut(s)	104
BsrDI	GCAATG	1 cut(s)	894
BsrI	ACTGG	5 cut(s)	286, 486, 736, 957, 1024
BssMI	GATC	3 cut(s)	433, 856, 1222
BssNAI	GTATAC	1 cut(s)	995
BssNI	GRCGYC	1 cut(s)	191
Bst1107I	GTATAC	1 cut(s)	995
Bst2UI	CCWGG	2 cut(s)	83, 706
Bst4CI	ACNGT	3 cut(s)	992, 1072, 1351
Bst6I	CTCTTC	3 cut(s)	111, 267, 995
BstACI	GRCGYC	1 cut(s)	191
BstDEI	CTNAG	6 cut(s)	56, 139, 213, 820, 962, 1128
BstF5I	GGATG	1 cut(s)	653
BstFNI	CGCG	2 cut(s)	249, 267
BstKTI	GATC	3 cut(s)	436, 859, 1225
BstMAI	GTCTC	3 cut(s)	27, 59, 654
BstMBI	GATC	3 cut(s)	433, 856, 1222
BstMWI	GCNNNNNNNGC	2 cut(s)	307, 1267
BstNI	CCWGG	2 cut(s)	83, 706
BstSCI	CCNGG	2 cut(s)	81, 704
BstSFI	CTRYAG	1 cut(s)	598
BstUI	CGCG	2 cut(s)	249, 267
BstV1I	GCAGC	2 cut(s)	134, 429
BstX2I	RGATCY	1 cut(s)	1222
BstYI	RGATCY	1 cut(s)	1222
BstZ17I	GTATAC	1 cut(s)	995
BsuI	GTATCC	1 cut(s)	923
BsuRI	GGCC	5 cut(s)	44, 528, 645, 792, 1023
BtsCI	GGATG	1 cut(s)	653
BtsIMutI	CAGTG	3 cut(s)	443, 950, 1068
CaiI	CAGNNNCTG	1 cut(s)	442
CciI	TCATGA	1 cut(s)	1081
Cfr13I	GGNCC	3 cut(s)	43, 644, 717
CseI	GACGC	1 cut(s)	180
Csp6I	GTAC	1 cut(s)	830
CviAII	CATG	6 cut(s)	470, 505, 589, 901, 1082, 1325
CviQI	GTAC	1 cut(s)	830
DdeI	CTNAG	6 cut(s)	56, 139, 213, 820, 962, 1128
DpnI	GATC	3 cut(s)	435, 858, 1224
DpnII	GATC	3 cut(s)	433, 856, 1222
DraI	TTTAAA	1 cut(s)	1096
DraIII	CACNNNGTG	1 cut(s)	1169
EaeI	YGGCCR	2 cut(s)	526, 1021
Eam1104I	CTCTTC	3 cut(s)	111, 267, 995
EarI	CTCTTC	3 cut(s)	111, 267, 995
Eco31I	GGTCTC	2 cut(s)	27, 59
Eco32I	GATATC	1 cut(s)	1357
Eco47I	GGWCC	1 cut(s)	717
Eco57I	CTGAAG	1 cut(s)	1019
Eco88I	CYCGRG	2 cut(s)	95, 657
EcoO109I	RGGNCCY	1 cut(s)	644
EcoRI	GAATTC	1 cut(s)	270
EcoRII	CCWGG	2 cut(s)	81, 704
EcoRV	GATATC	1 cut(s)	1357
FaeI	CATG	6 cut(s)	473, 508, 592, 904, 1085, 1328
FalI	AAGNNNNNCTT	2 cut(s)	246, 278
FaqI	GGGAC	1 cut(s)	1225
FatI	CATG	6 cut(s)	469, 504, 588, 900, 1081, 1324
FblI	GTMKAC	1 cut(s)	994
Fnu4HI	GCNGC	3 cut(s)	123, 423, 443
FokI	GGATG	1 cut(s)	640
Fsp4HI	GCNGC	3 cut(s)	123, 423, 443
FspBI	CTAG	2 cut(s)	641, 1166
GluI	GCNGC	3 cut(s)	123, 423, 443
GsaI	CCCAGC	2 cut(s)	49, 1421
GsuI	CTGGAG	1 cut(s)	323
HaeIII	GGCC	5 cut(s)	44, 528, 645, 792, 1023
HapII	CCGG	4 cut(s)	66, 223, 275, 446
HgaI	GACGC	1 cut(s)	180
Hin1I	GRCGYC	1 cut(s)	191
Hin1II	CATG	6 cut(s)	473, 508, 592, 904, 1085, 1328
HinfI	GANTC	5 cut(s)	98, 182, 320, 566, 1280
HpaII	CCGG	4 cut(s)	66, 223, 275, 446
HphI	GGTGA	3 cut(s)	497, 1181, 1400
Hpy166II	GTNNAC	2 cut(s)	595, 995
Hpy188I	TCNGA	8 cut(s)	32, 325, 571, 697, 721, 1131, 1285, 1365
Hpy188III	TCNNGA	6 cut(s)	223, 317, 563, 657, 1082, 1380
Hpy8I	GTNNAC	2 cut(s)	595, 995
Hpy99I	CGWCG	1 cut(s)	137
HpyAV	CCTTC	5 cut(s)	153, 286, 361, 368, 782
HpyCH4III	ACNGT	3 cut(s)	992, 1072, 1351
HpyCH4IV	ACGT	1 cut(s)	1399
HpyCH4V	TGCA	8 cut(s)	336, 478, 806, 891, 1184, 1315, 1328, 1376
HpyF10VI	GCNNNNNNNGC	2 cut(s)	307, 1267
HpyF3I	CTNAG	6 cut(s)	56, 139, 213, 820, 962, 1128
HpySE526I	ACGT	1 cut(s)	1399
Hsp92I	GRCGYC	1 cut(s)	191
Hsp92II	CATG	6 cut(s)	473, 508, 592, 904, 1085, 1328
Kpn2I	TCCGGA	1 cut(s)	222
Kzo9I	GATC	3 cut(s)	433, 856, 1222
LguI	GCTCTTC	1 cut(s)	111
Lsp1109I	GCAGC	2 cut(s)	134, 429
LweI	GCATC	3 cut(s)	337, 490, 1385
MaeI	CTAG	2 cut(s)	641, 1166
MaeII	ACGT	1 cut(s)	1399
MaeIII	GTNAC	3 cut(s)	400, 636, 986
MalI	GATC	3 cut(s)	435, 858, 1224
MbiI	CCGCTC	1 cut(s)	104
MboI	GATC	3 cut(s)	433, 856, 1222
MboII	GAAGA	7 cut(s)	98, 185, 254, 383, 406, 1012, 1232
MfeI	CAATTG	2 cut(s)	38, 669
MflI	RGATCY	1 cut(s)	1222
MlsI	TGGCCA	2 cut(s)	528, 1023
MluNI	TGGCCA	2 cut(s)	528, 1023
MlyI	GAGTC	1 cut(s)	107
MmeI	TCCRAC	2 cut(s)	411, 1128
Mox20I	TGGCCA	2 cut(s)	528, 1023
MroI	TCCGGA	1 cut(s)	222
MscI	TGGCCA	2 cut(s)	528, 1023
MseI	TTAA	2 cut(s)	1052, 1095
MslI	CAYNNNNRTG	2 cut(s)	468, 899
Msp20I	TGGCCA	2 cut(s)	528, 1023
MspA1I	CMGCKG	1 cut(s)	800
MspI	CCGG	4 cut(s)	66, 223, 275, 446
MspR9I	CCNGG	2 cut(s)	83, 706
MunI	CAATTG	2 cut(s)	38, 669
Mva1269I	GAATGC	1 cut(s)	1336
MvaI	CCWGG	2 cut(s)	83, 706
MvnI	CGCG	2 cut(s)	249, 267
MwoI	GCNNNNNNNGC	2 cut(s)	307, 1267
NdeII	GATC	3 cut(s)	433, 856, 1222
NlaIII	CATG	6 cut(s)	473, 508, 592, 904, 1085, 1328
NlaIV	GGNNCC	3 cut(s)	70, 286, 1276
PaeR7I	CTCGAG	1 cut(s)	95
PagI	TCATGA	1 cut(s)	1081
PciSI	GCTCTTC	1 cut(s)	111
PcsI	WCGNNNNNNNCGW	1 cut(s)	873
PctI	GAATGC	1 cut(s)	1336
PfeI	GAWTC	4 cut(s)	182, 320, 566, 1280
PkrI	GCNGC	3 cut(s)	124, 424, 444
PleI	GAGTC	1 cut(s)	106
PpsI	GAGTC	1 cut(s)	106
Psp1406I	AACGTT	1 cut(s)	1399
Psp6I	CCWGG	2 cut(s)	81, 704
PspFI	CCCAGC	2 cut(s)	45, 1417
PspGI	CCWGG	2 cut(s)	81, 704
PspN4I	GGNNCC	3 cut(s)	70, 286, 1276
PspPI	GGNCC	3 cut(s)	43, 644, 717
PspXI	VCTCGAGB	1 cut(s)	95
PstNI	CAGNNNCTG	1 cut(s)	442
PsuI	RGATCY	1 cut(s)	1222
PvuII	CAGCTG	1 cut(s)	800
RsaI	GTAC	1 cut(s)	831
RsaNI	GTAC	1 cut(s)	830
RseI	CAYNNNNRTG	2 cut(s)	468, 899
SapI	GCTCTTC	1 cut(s)	111
SaqAI	TTAA	2 cut(s)	1052, 1095
SatI	GCNGC	3 cut(s)	123, 423, 443
Sau3AI	GATC	3 cut(s)	433, 856, 1222
Sau96I	GGNCC	3 cut(s)	43, 644, 717
SchI	GAGTC	1 cut(s)	107
ScrFI	CCNGG	2 cut(s)	83, 706
SfaNI	GCATC	3 cut(s)	337, 490, 1385
SfcI	CTRYAG	1 cut(s)	598
Sfr274I	CTCGAG	1 cut(s)	95
SinI	GGWCC	1 cut(s)	717
SlaI	CTCGAG	1 cut(s)	95
SmiMI	CAYNNNNRTG	2 cut(s)	468, 899
SmlI	CTYRAG	1 cut(s)	95
SmoI	CTYRAG	1 cut(s)	95
SsiI	CCGC	7 cut(s)	63, 102, 120, 247, 267, 422, 1268
SspMI	CTAG	2 cut(s)	641, 1166
StyD4I	CCNGG	2 cut(s)	81, 704
TaaI	ACNGT	3 cut(s)	992, 1072, 1351
TaiI	ACGT	1 cut(s)	1402
TaqI	TCGA	2 cut(s)	89, 96
TauI	GCSGC	1 cut(s)	425
TfiI	GAWTC	4 cut(s)	182, 320, 566, 1280
Tru1I	TTAA	2 cut(s)	1052, 1095
Tru9I	TTAA	2 cut(s)	1052, 1095
TscAI	CASTG	3 cut(s)	443, 957, 1075
TseI	GCWGC	2 cut(s)	122, 442
TspDTI	ATGAA	5 cut(s)	186, 245, 577, 920, 1302
TspGWI	ACGGA	1 cut(s)	1361
TspRI	CASTG	3 cut(s)	443, 957, 1075
VpaK11BI	GGWCC	1 cut(s)	717
XapI	RAATTY	4 cut(s)	270, 677, 689, 1289
XcmI	CCANNNNNNNNNTGG	2 cut(s)	79, 521
XhoI	CTCGAG	1 cut(s)	95
XmiI	GTMKAC	1 cut(s)	994
XspI	CTAG	2 cut(s)	641, 1166

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.