Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr03

Orthogroup ID

OG0006576

Physical Location & Seq

Genomic Coordinates Reverse (-)

36776396 .. 36781877

Gene Structure 5482 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

MD03G1289600.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 2166 bp

ATGGCGTTTTGGTGTTTGTTTGACTCGGTTTATATAAGACCCGAATTAATTCGGCACAAACCCGGTGGGCCTGACCATAGCATTGGGACTTTGAGCACAAGTCGAATAACTTGTAAAACTAAAAGCCCGCAGAAAGCCCGCCAAAAGAAGCTTGCCGCGGACGCTGGGGAGAAAGAAGACCTCCACACTGTAACTCCCGCCTCAAAATCCCTCCTCCGCTTCACAGTTTCAACCATCTCTCCCCCAATGGCAAGCAAGCTCAAAGTAGAAGAGCTCCGAAACGAATTGGCTCAGCGTGGTCTCCCCACCACCGGAGCGAAGCCCACCCTGGTTCGGAGGCTCGATTCTGCTCTTCGCGAGGAGAAGAAGCAACCCACAGGCGCAAGTGATGGTTCAGCAGCAGGAGCTTCACTGGTTAGTAGAAAGAGGGAAAGGAAGTTAATTGAAGAGGAGGGAGGTGGGGATTCAAATGTGTCCGAGAAAATGAAGGCCACCGAAAAGTTTAGGGGCATGAGCGTGAAGCAATTGCGCGAAGAAGCCGCTCTTTGTGGAGTTTCGGCAACTGGGTCGAAAAAAGAATTGCTGGAGAGGCTTTCTGAACATTCCGATGGCATTCCTCTAGGTAAAGCTAATGAGGAAGGAAATGGCGGCAAGGAGAAGATAATAACCGCAACCAAAAAGGGTGCGGCGGTACTGGATCAGTGGCTACCGGATGACATAAGGGCACATTATCATGTTTTGCAACTGGGGGATGATATATACGATGCCATGTTAAACCAGACCAATGTCGGTCATAACAATAACAAGTTCTATCTGATTCAAGTTCTTGAATCTGATGTTGGTGGAAGTTTCATGATTTACTATAGATGGGGGAGAGTAGGTGTCAAGGGTCAGAACAAGCTAGCTCCTCATGCATCCCGTGAGAGCGCGATCAATGAGTTTAAACAGAAATTCTATGACAAAACCAGGAATGATTGGTCCAATCGAAAAATGTTCCAGCCTATCCCATCCTGCTATATGTGGATAGAAATGGATTACAATGAAAAGGAAGAGCAGTCAGCTGTTGAAGAAAAGAATGGCTCTGCTTTAGGACGTCAACCTTTGGAAACACAACTTGAGCCCTGCATTGCAAAGTTTATATCTCTTATTTGCAACATCGGCATGATGAAGCAACATATGATGGAAATAGGATACAACGCTGATAAGTTGCCTCTGGGTAAACTTAGCAAATCAACAATTCTAAAGGGCTATAACATCCTGCAGAGGATTTCTGATGTGATTGGCTCATCTAACAGGAAATTAATCGAACAATTAAGCGGAGAGTTCTACACTGTCATTCCTCATGACTTCGGGTTTAAAAAAATGCATAACTTTGTGATTGATACTCCTCAGAAGTTGAAACACAAGCTAGAAATGGTTGAAGCACTAGGTGAAATTGAGGTTGCAACGAAATTGTTGAAGGATGACACAGGAATGCAGGGAGATCCCTTGTATTCCAGTTATCAACGCCTTCATTGTGAGCTGACACCGGTTGGTGCTGATTCTCATGAATTTGATATGATTACAAAATATTTGCATAATACTCATGCAAAAACACATTCAACTTACACTGTTGATATTGTTCAAATATTCCGCACATCTAAAGAGGGTGAAGTTGAACGCTTCAGAAAGTTTTCTAGTACGAAAAATAGAATGCTTTTGTGGCATGGTTCTCGTCTTACAAATTGGGCTGGCATATTATCTCAAGGCTTGCGCATAGCTCCACCTGAAGCCCCAGTGACTGGTTACATGTTCGGGAAAGGAGTTTACTTCGCAGACATGTTCTCCAAAAGTGCAAATTATTGCTATGCATCTAATGGCTGTACATCTGGGGTGCTGCTTCTATGTGAGGTTGCGCTGGGCGACATGGCTGAGCTTTTAACCGCTAAGTACGATGCTGACAAGTTACCTGTAGGAAAACTAAGCACTAAAGGAGTTGGGGGAACTGAACCAGATTTTTCAGAAGCTCAGTTACTAGATGATGGTGTCGTGGTTCCCCTAGGAAAGCCGAAAAAGAATACAAGCCGCCCGAAGGGTTCATTACTGTACAATGAGTACATAGTTTACAATGTGGAACAGATCAGGATGCGCTACGTTGTTCAAGTTAATTTCAATTTCAAGTTATGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000726 GO:0003674 GO:0003824 GO:0003909 GO:0003910 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005737 GO:0006139 GO:0006259 GO:0006260 GO:0006261 GO:0006266 GO:0006271 GO:0006273 GO:0006281 GO:0006302 GO:0006303 GO:0006725 GO:0006807 GO:0006950 GO:0006974 GO:0008150 GO:0008152 GO:0009058 GO:0009059 GO:0009987 GO:0016070 GO:0016874 GO:0016886 GO:0022616 GO:0033554 GO:0034641 GO:0034645 GO:0043170 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044237 GO:0044238 GO:0044249 GO:0044260 GO:0044424 GO:0044464 GO:0046483 GO:0050896 GO:0051103 GO:0051716 GO:0071704 GO:0090304 GO:0140097 GO:1901360 GO:1901576

KEGG Pathways

Metabolic & Signaling

ko03410 ko04210 ko04212 ko04214 ko04217 map03410 map04210 map04212 map04214 map04217

Pfam Domains

Protein Families

PARP PARP_reg SAP WGR

Protein Analysis

722

Amino Acids

80.6

Weight (kDa)

8.95

Isoelectric Point (pI)

38.74

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
SAP	PF02037	84 - 119	3.7e-10	SAP domain
SAP	PF02037	168 - 201	1.7e-06	SAP domain
WGR	PF05406	254 - 332	5.5e-25	WGR domain
PARP_reg	PF02877	371 - 501	1.5e-39	Poly(ADP-ribose) polymerase, regulatory domain
PARP	PF00644	515 - 717	1.6e-80	Poly(ADP-ribose) polymerase catalytic domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0006576)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT4G02390 AT4G02390 AT4G02390
fragaria_vesca	FvH4_2g25840 FvH4_2g25840 FvH4_2g25840
malus_domestica	MD03G1289400.v1.1 MD03G1289500.v1.1 MD03G1289600.v1.1
prunus_persica	Prupe.8G262600_v2.0.a1 Prupe.8G262600_v2.0.a1 Prupe.8G262600_v2.0.a1 Prupe.8G262600_v2.0.a1 Prupe.8G262600_v2.0.a1
pyrus_communis	pycom03g22920
rosa_chinensis	RchiOBHm_Chr6g0294171
rosa_laevigata	RLG00000011893
rosa_multiflora	Rmu_sc0001521.1_g000044
rosa_roxburghii	Rroxscaffold_7G00173580
rosa_rugosa	Rorug06G0242000 Rorug06G0242100
rosa_samantha	Rh6AG354300 Rh6BG361200 Rh6CG368500 Rh6DG355300
rosa_wichuraiana	Rw6G030880

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AatII	GACGTC	1 cut(s)	1096
Acc16I	TGCGCA	1 cut(s)	1754
AccB7I	CCANNNNNTGG	1 cut(s)	1781
AccBSI	CCGCTC	1 cut(s)	542
AccII	CGCG	4 cut(s)	158, 357, 531, 929
AclWI	GGATC	2 cut(s)	705, 1478
AcsI	RAATTY	2 cut(s)	950, 1550
AcuI	CTGAAG	2 cut(s)	1648, 1788
AcyI	GRCGYC	1 cut(s)	1093
AdeI	CACNNNGTG	1 cut(s)	1430
AfaI	GTAC	6 cut(s)	693, 1681, 1864, 1931, 2087, 2096
AfiI	CCNNNNNNNGG	4 cut(s)	311, 333, 1781, 2071
AflIII	ACRYGT	2 cut(s)	1788, 1818
AgeI	ACCGGT	1 cut(s)	1528
AjnI	CCWGG	2 cut(s)	327, 965
AjuI	GAANNNNNNNTTGG	2 cut(s)	822, 854
Alw21I	GWGCWC	2 cut(s)	98, 276
Alw26I	GTCTC	1 cut(s)	305
AlwI	GGATC	2 cut(s)	705, 1478
AlwNI	CAGNNNCTG	1 cut(s)	1781
AoxI	GGCC	2 cut(s)	68, 489
ApeKI	GCWGC	2 cut(s)	398, 1876
ApoI	RAATTY	2 cut(s)	950, 1550
AseI	ATTAAT	2 cut(s)	47, 1301
AsiGI	ACCGGT	1 cut(s)	1528
Asp700I	GAANNNNTTC	2 cut(s)	48, 1672
AspA2I	CCTAGG	1 cut(s)	2038
AspLEI	GCGC	6 cut(s)	383, 531, 929, 1755, 1897, 2130
AspS9I	GGNCC	2 cut(s)	68, 978
AsuC2I	CCSGG	1 cut(s)	63
AsuHPI	GGTGA	2 cut(s)	1442, 1661
AsuNHI	GCTAGC	1 cut(s)	901
AvaII	GGWCC	1 cut(s)	978
AvrII	CCTAGG	1 cut(s)	2038
BaeGI	GKGCMC	1 cut(s)	727
BanII	GRGCYC	2 cut(s)	276, 1122
BarI	GAAGNNNNNNTAC	2 cut(s)	1995, 2027
BbsI	GAAGAC	1 cut(s)	183
Bbv12I	GWGCWC	2 cut(s)	98, 276
BbvI	GCAGC	2 cut(s)	410, 1863
BccI	CCATC	7 cut(s)	242, 383, 602, 861, 1015, 1174, 2015
BcgI	CGANNNNNNTGC	4 cut(s)	549, 583, 1694, 1728
BciT130I	CCWGG	2 cut(s)	329, 967
BciVI	GTATCC	1 cut(s)	1184
BcnI	CCSGG	1 cut(s)	63
BcoDI	GTCTC	1 cut(s)	305
BfaI	CTAG	7 cut(s)	620, 902, 1409, 1427, 1677, 2015, 2039
BfmI	CTRYAG	3 cut(s)	862, 1259, 1950
BfuI	GTATCC	1 cut(s)	1184
BisI	GCNGC	7 cut(s)	156, 399, 540, 649, 687, 1877, 2065
BlnI	CCTAGG	1 cut(s)	2038
BlpI	GCTNAGC	2 cut(s)	291, 1911
BlsI	GCNGC	7 cut(s)	157, 400, 541, 650, 688, 1878, 2066
Bme1390I	CCNGG	3 cut(s)	63, 329, 967
Bme18I	GGWCC	1 cut(s)	978
BmgT120I	GGNCC	2 cut(s)	68, 978
BmiI	GGNNCC	1 cut(s)	2034
BmrFI	CCNGG	3 cut(s)	63, 329, 967
BmrI	ACTGGG	3 cut(s)	573, 755, 1769
BmsI	GCATC	5 cut(s)	754, 923, 1859, 1924, 2115
BmtI	GCTAGC	1 cut(s)	905
BmuI	ACTGGG	3 cut(s)	573, 755, 1769
BoxI	GACNNNNGTC	1 cut(s)	785
BpiI	GAAGAC	1 cut(s)	183
BpmI	CTGGAG	1 cut(s)	605
Bpu1102I	GCTNAGC	2 cut(s)	291, 1911
BpuEI	CTTGAG	2 cut(s)	1136, 1728
BpuMI	CCSGG	1 cut(s)	63
BsaHI	GRCGYC	1 cut(s)	1093
BsaI	GGTCTC	1 cut(s)	305
BsaJI	CCNNGG	3 cut(s)	156, 327, 2038
BsaWI	WCCGGW	3 cut(s)	311, 709, 1528
BsaXI	ACNNNNNCTCC	8 cut(s)	178, 208, 223, 253, 865, 895, 1808, 1838
Bsc4I	CCNNNNNNNGG	4 cut(s)	311, 333, 1781, 2071
Bse118I	RCCGGY	1 cut(s)	1528
Bse1I	ACTGG	7 cut(s)	417, 568, 699, 750, 1497, 1775, 1786
Bse3DI	GCAATG	1 cut(s)	1125
BseBI	CCWGG	2 cut(s)	329, 967
BseDI	CCNNGG	3 cut(s)	156, 327, 2038
BseGI	GGATG	7 cut(s)	718, 757, 914, 1007, 1254, 1468, 2130
BseLI	CCNNNNNNNGG	4 cut(s)	311, 333, 1781, 2071
BseMI	GCAATG	1 cut(s)	1125
BseMII	CTCAG	4 cut(s)	305, 1403, 1902, 2021
BseNI	ACTGG	7 cut(s)	417, 568, 699, 750, 1497, 1775, 1786
BseRI	GAGGAG	5 cut(s)	203, 374, 464, 897, 1377
BseSI	GKGCMC	1 cut(s)	727
BseXI	GCAGC	2 cut(s)	410, 1863
BseYI	CCCAGC	2 cut(s)	164, 1897
Bsh1236I	CGCG	4 cut(s)	158, 357, 531, 929
BshFI	GGCC	2 cut(s)	70, 491
BshTI	ACCGGT	1 cut(s)	1528
BsiHKAI	GWGCWC	2 cut(s)	98, 276
BsiSI	CCGG	4 cut(s)	63, 312, 710, 1529
BslFI	GGGAC	1 cut(s)	100
BslI	CCNNNNNNNGG	4 cut(s)	311, 333, 1781, 2071
BsmAI	GTCTC	1 cut(s)	305
BsmFI	GGGAC	1 cut(s)	100
BsmI	GAATGC	3 cut(s)	612, 1479, 1698
BsnI	GGCC	2 cut(s)	70, 491
Bso31I	GGTCTC	1 cut(s)	305
Bsp1286I	GDGCHC	4 cut(s)	98, 276, 727, 1122
Bsp1407I	TGTACA	2 cut(s)	1862, 2085
Bsp143I	GATC	4 cut(s)	697, 930, 1483, 2118
Bsp1720I	GCTNAGC	2 cut(s)	291, 1911
Bsp68I	TCGCGA	1 cut(s)	357
BspANI	GGCC	2 cut(s)	70, 491
BspCNI	CTCAG	4 cut(s)	304, 1402, 1903, 2020
BspFNI	CGCG	4 cut(s)	158, 357, 531, 929
BspHI	TCATGA	3 cut(s)	852, 1342, 1546
BspLI	GGNNCC	1 cut(s)	2034
BspMAI	CTGCAG	1 cut(s)	1263
BspOI	GCTAGC	1 cut(s)	905
BspPI	GGATC	2 cut(s)	705, 1478
BspQI	GCTCTTC	3 cut(s)	264, 357, 1044
BspTNI	GGTCTC	1 cut(s)	305
BsrBI	CCGCTC	1 cut(s)	542
BsrDI	GCAATG	1 cut(s)	1125
BsrFI	RCCGGY	1 cut(s)	1528
BsrGI	TGTACA	2 cut(s)	1862, 2085
BsrI	ACTGG	7 cut(s)	417, 568, 699, 750, 1497, 1775, 1786
BssAI	RCCGGY	1 cut(s)	1528
BssECI	CCNNGG	3 cut(s)	156, 327, 2038
BssMI	GATC	4 cut(s)	697, 930, 1483, 2118
BssNI	GRCGYC	1 cut(s)	1093
BssT1I	CCWWGG	1 cut(s)	2038
Bst2UI	CCWGG	2 cut(s)	329, 967
Bst4CI	ACNGT	5 cut(s)	190, 226, 1333, 1612, 2085
Bst6I	CTCTTC	4 cut(s)	264, 357, 441, 1044
BstACI	GRCGYC	1 cut(s)	1093
BstAUI	TGTACA	2 cut(s)	1862, 2085
BstC8I	GCNNGC	8 cut(s)	128, 139, 153, 253, 257, 903, 1732, 1751
BstDEI	CTNAG	7 cut(s)	291, 1223, 1389, 1911, 1926, 1961, 2007
BstDSI	CCRYGG	1 cut(s)	156
BstF5I	GGATG	7 cut(s)	718, 757, 914, 1007, 1254, 1468, 2130
BstFNI	CGCG	4 cut(s)	158, 357, 531, 929
BstHHI	GCGC	6 cut(s)	383, 531, 929, 1755, 1897, 2130
BstKTI	GATC	4 cut(s)	700, 933, 1486, 2121
BstMAI	GTCTC	1 cut(s)	305
BstMBI	GATC	4 cut(s)	697, 930, 1483, 2118
BstMWI	GCNNNNNNNGC	6 cut(s)	161, 404, 589, 911, 1158, 1702
BstNI	CCWGG	2 cut(s)	329, 967
BstNSI	RCATGY	2 cut(s)	1792, 1822
BstPAI	GACNNNNGTC	1 cut(s)	785
BstSCI	CCNGG	3 cut(s)	61, 327, 965
BstSFI	CTRYAG	3 cut(s)	862, 1259, 1950
BstSLI	GKGCMC	1 cut(s)	727
BstUI	CGCG	4 cut(s)	158, 357, 531, 929
BstV1I	GCAGC	2 cut(s)	410, 1863
BstV2I	GAAGAC	1 cut(s)	183
BstX2I	RGATCY	1 cut(s)	1483
BstXI	CCANNNNNNTGG	1 cut(s)	83
BstYI	RGATCY	1 cut(s)	1483
BsuI	GTATCC	1 cut(s)	1184
BsuRI	GGCC	2 cut(s)	70, 491
BtgI	CCRYGG	1 cut(s)	156
BtsCI	GGATG	7 cut(s)	718, 757, 914, 1007, 1254, 1468, 2130
BtsIMutI	CAGTG	6 cut(s)	186, 410, 707, 1329, 1608, 1782
BtuMI	TCGCGA	1 cut(s)	357
Cac8I	GCNNGC	8 cut(s)	128, 139, 153, 253, 257, 903, 1732, 1751
CaiI	CAGNNNCTG	1 cut(s)	1781
CciI	TCATGA	3 cut(s)	852, 1342, 1546
CfoI	GCGC	6 cut(s)	383, 531, 929, 1755, 1897, 2130
Cfr10I	RCCGGY	1 cut(s)	1528
Cfr13I	GGNCC	2 cut(s)	68, 978
Cfr42I	CCGCGG	1 cut(s)	159
CseI	GACGC	1 cut(s)	170
Csp6I	GTAC	6 cut(s)	692, 1680, 1863, 1930, 2086, 2095
CspAI	ACCGGT	1 cut(s)	1528
CspCI	CAANNNNNGTGG	2 cut(s)	46, 81
CviQI	GTAC	6 cut(s)	692, 1680, 1863, 1930, 2086, 2095
DdeI	CTNAG	7 cut(s)	291, 1223, 1389, 1911, 1926, 1961, 2007
DpnI	GATC	4 cut(s)	699, 932, 1485, 2120
DpnII	GATC	4 cut(s)	697, 930, 1483, 2118
DraI	TTTAAA	2 cut(s)	943, 1357
DraIII	CACNNNGTG	1 cut(s)	1430
Eam1104I	CTCTTC	4 cut(s)	264, 357, 441, 1044
EarI	CTCTTC	4 cut(s)	264, 357, 441, 1044
Ecl136II	GAGCTC	1 cut(s)	274
Eco130I	CCWWGG	1 cut(s)	2038
Eco24I	GRGCYC	2 cut(s)	276, 1122
Eco31I	GGTCTC	1 cut(s)	305
Eco47I	GGWCC	1 cut(s)	978
Eco53kI	GAGCTC	1 cut(s)	274
Eco57I	CTGAAG	2 cut(s)	1648, 1788
EcoICRI	GAGCTC	1 cut(s)	274
EcoRII	CCWGG	2 cut(s)	327, 965
EcoT14I	CCWWGG	1 cut(s)	2038
EcoT22I	ATGCAT	3 cut(s)	916, 1368, 1852
EcoT38I	GRGCYC	2 cut(s)	276, 1122
ErhI	CCWWGG	1 cut(s)	2038
FalI	AAGNNNNNCTT	2 cut(s)	528, 560
FaqI	GGGAC	1 cut(s)	100
FauI	CCCGC	3 cut(s)	135, 146, 205
FauNDI	CATATG	1 cut(s)	1176
Fnu4HI	GCNGC	7 cut(s)	156, 399, 540, 649, 687, 1877, 2065
FokI	GGATG	7 cut(s)	725, 764, 901, 994, 1241, 1475, 2137
FriOI	GRGCYC	2 cut(s)	276, 1122
Fsp4HI	GCNGC	7 cut(s)	156, 399, 540, 649, 687, 1877, 2065
FspBI	CTAG	7 cut(s)	620, 902, 1409, 1427, 1677, 2015, 2039
FspI	TGCGCA	1 cut(s)	1754
GlaI	GCGC	6 cut(s)	382, 530, 928, 1754, 1896, 2129
GluI	GCNGC	7 cut(s)	156, 399, 540, 649, 687, 1877, 2065
GsaI	CCCAGC	2 cut(s)	168, 1901
GsuI	CTGGAG	1 cut(s)	605
HaeIII	GGCC	2 cut(s)	70, 491
HapII	CCGG	4 cut(s)	63, 312, 710, 1529
HgaI	GACGC	1 cut(s)	170
HhaI	GCGC	6 cut(s)	383, 531, 929, 1755, 1897, 2130
Hin1I	GRCGYC	1 cut(s)	1093
Hin6I	GCGC	6 cut(s)	381, 529, 927, 1753, 1895, 2128
HinP1I	GCGC	6 cut(s)	381, 529, 927, 1753, 1895, 2128
HincII	GTYRAC	1 cut(s)	1097
HindII	GTYRAC	1 cut(s)	1097
HindIII	AAGCTT	1 cut(s)	149
HinfI	GANTC	6 cut(s)	23, 344, 464, 817, 830, 1541
HpaII	CCGG	4 cut(s)	63, 312, 710, 1529
HphI	GGTGA	2 cut(s)	1442, 1661
Hpy166II	GTNNAC	4 cut(s)	1097, 1220, 1807, 2104
Hpy188III	TCNNGA	7 cut(s)	356, 827, 853, 1343, 1547, 1795, 2122
Hpy8I	GTNNAC	4 cut(s)	1097, 1220, 1807, 2104
HpyAV	CCTTC	5 cut(s)	481, 632, 1453, 1520, 2065
HpyCH4III	ACNGT	5 cut(s)	190, 226, 1333, 1612, 2085
HpyCH4IV	ACGT	2 cut(s)	1093, 2133
HpyF10VI	GCNNNNNNNGC	6 cut(s)	161, 404, 589, 911, 1158, 1702
HpyF3I	CTNAG	7 cut(s)	291, 1223, 1389, 1911, 1926, 1961, 2007
HpySE526I	ACGT	2 cut(s)	1093, 2133
Hsp92I	GRCGYC	1 cut(s)	1093
HspAI	GCGC	6 cut(s)	381, 529, 927, 1753, 1895, 2128
KspI	CCGCGG	1 cut(s)	159
Kzo9I	GATC	4 cut(s)	697, 930, 1483, 2118
LguI	GCTCTTC	3 cut(s)	264, 357, 1044
LmnI	GCTCC	5 cut(s)	279, 314, 404, 910, 1765
Lsp1109I	GCAGC	2 cut(s)	410, 1863
LweI	GCATC	5 cut(s)	754, 923, 1859, 1924, 2115
MaeI	CTAG	7 cut(s)	620, 902, 1409, 1427, 1677, 2015, 2039
MaeII	ACGT	2 cut(s)	1093, 2133
MaeIII	GTNAC	5 cut(s)	190, 1777, 1784, 1944, 2010
MalI	GATC	4 cut(s)	699, 932, 1485, 2120
MbiI	CCGCTC	1 cut(s)	542
MboI	GATC	4 cut(s)	697, 930, 1483, 2118
MboII	GAAGA	9 cut(s)	188, 281, 344, 376, 458, 545, 670, 1061, 1079
MfeI	CAATTG	1 cut(s)	524
MflI	RGATCY	1 cut(s)	1483
MhlI	GDGCHC	4 cut(s)	98, 276, 727, 1122
MlyI	GAGTC	1 cut(s)	17
Mph1103I	ATGCAT	3 cut(s)	916, 1368, 1852
MroXI	GAANNNNTTC	2 cut(s)	48, 1672
MseI	TTAA	9 cut(s)	47, 440, 773, 942, 1301, 1313, 1356, 1919, 2145
MslI	CAYNNNNRTG	5 cut(s)	515, 606, 732, 1160, 1472
MspA1I	CMGCKG	2 cut(s)	158, 1061
MspI	CCGG	4 cut(s)	63, 312, 710, 1529
MspR9I	CCNGG	3 cut(s)	63, 329, 967
MssI	GTTTAAAC	1 cut(s)	943
MunI	CAATTG	1 cut(s)	524
Mva1269I	GAATGC	3 cut(s)	612, 1479, 1698
MvaI	CCWGG	2 cut(s)	329, 967
MvnI	CGCG	4 cut(s)	158, 357, 531, 929
MwoI	GCNNNNNNNGC	6 cut(s)	161, 404, 589, 911, 1158, 1702
NciI	CCSGG	1 cut(s)	63
NdeI	CATATG	1 cut(s)	1176
NdeII	GATC	4 cut(s)	697, 930, 1483, 2118
NheI	GCTAGC	1 cut(s)	901
NlaIV	GGNNCC	1 cut(s)	2034
NmuCI	GTSAC	1 cut(s)	1777
NruI	TCGCGA	1 cut(s)	357
NsbI	TGCGCA	1 cut(s)	1754
NsiI	ATGCAT	3 cut(s)	916, 1368, 1852
NspI	RCATGY	2 cut(s)	1792, 1822
PagI	TCATGA	3 cut(s)	852, 1342, 1546
PciI	ACATGT	2 cut(s)	1788, 1818
PciSI	GCTCTTC	3 cut(s)	264, 357, 1044
PctI	GAATGC	3 cut(s)	612, 1479, 1698
PdmI	GAANNNNTTC	2 cut(s)	48, 1672
PfeI	GAWTC	5 cut(s)	344, 464, 817, 830, 1541
PflMI	CCANNNNNTGG	1 cut(s)	1781
PinAI	ACCGGT	1 cut(s)	1528
PkrI	GCNGC	7 cut(s)	157, 400, 541, 650, 688, 1878, 2066
PleI	GAGTC	1 cut(s)	17
PmeI	GTTTAAAC	1 cut(s)	943
PpsI	GAGTC	1 cut(s)	17
PscI	ACATGT	2 cut(s)	1788, 1818
PshAI	GACNNNNGTC	1 cut(s)	785
PshBI	ATTAAT	2 cut(s)	47, 1301
Psp124BI	GAGCTC	1 cut(s)	276
Psp6I	CCWGG	2 cut(s)	327, 965
PspFI	CCCAGC	2 cut(s)	164, 1897
PspGI	CCWGG	2 cut(s)	327, 965
PspN4I	GGNNCC	1 cut(s)	2034
PspPI	GGNCC	2 cut(s)	68, 978
PstI	CTGCAG	1 cut(s)	1263
PstNI	CAGNNNCTG	1 cut(s)	1781
PsuI	RGATCY	1 cut(s)	1483
PvuII	CAGCTG	1 cut(s)	1061
RruI	TCGCGA	1 cut(s)	357
RsaI	GTAC	6 cut(s)	693, 1681, 1864, 1931, 2087, 2096
RsaNI	GTAC	6 cut(s)	692, 1680, 1863, 1930, 2086, 2095
RseI	CAYNNNNRTG	5 cut(s)	515, 606, 732, 1160, 1472
SacI	GAGCTC	1 cut(s)	276
SacII	CCGCGG	1 cut(s)	159
SapI	GCTCTTC	3 cut(s)	264, 357, 1044
SaqAI	TTAA	9 cut(s)	47, 440, 773, 942, 1301, 1313, 1356, 1919, 2145
SatI	GCNGC	7 cut(s)	156, 399, 540, 649, 687, 1877, 2065
Sau3AI	GATC	4 cut(s)	697, 930, 1483, 2118
Sau96I	GGNCC	2 cut(s)	68, 978
SchI	GAGTC	1 cut(s)	17
ScrFI	CCNGG	3 cut(s)	63, 329, 967
SduI	GDGCHC	4 cut(s)	98, 276, 727, 1122
SfaNI	GCATC	5 cut(s)	754, 923, 1859, 1924, 2115
SfcI	CTRYAG	3 cut(s)	862, 1259, 1950
Sfr303I	CCGCGG	1 cut(s)	159
SgrBI	CCGCGG	1 cut(s)	159
SinI	GGWCC	1 cut(s)	978
SmiMI	CAYNNNNRTG	5 cut(s)	515, 606, 732, 1160, 1472
SmlI	CTYRAG	2 cut(s)	1115, 1743
SmoI	CTYRAG	2 cut(s)	1115, 1743
SspI	AATATT	2 cut(s)	1571, 1629
SspMI	CTAG	7 cut(s)	620, 902, 1409, 1427, 1677, 2015, 2039
SstI	GAGCTC	1 cut(s)	276
StyD4I	CCNGG	3 cut(s)	61, 327, 965
StyI	CCWWGG	1 cut(s)	2038
TaaI	ACNGT	5 cut(s)	190, 226, 1333, 1612, 2085
TaiI	ACGT	2 cut(s)	1096, 2136
TaqI	TCGA	5 cut(s)	103, 342, 569, 985, 1305
TaqII	GACCGA	1 cut(s)	779
TatI	WGTACW	3 cut(s)	1862, 2085, 2094
TauI	GCSGC	5 cut(s)	158, 542, 651, 689, 2067
TfiI	GAWTC	5 cut(s)	344, 464, 817, 830, 1541
Tru1I	TTAA	9 cut(s)	47, 440, 773, 942, 1301, 1313, 1356, 1919, 2145
Tru9I	TTAA	9 cut(s)	47, 440, 773, 942, 1301, 1313, 1356, 1919, 2145
TscAI	CASTG	6 cut(s)	193, 417, 707, 1336, 1615, 1782
TseFI	GTSAC	1 cut(s)	1777
TseI	GCWGC	2 cut(s)	398, 1876
Tsp45I	GTSAC	1 cut(s)	1777
TspDTI	ATGAA	7 cut(s)	500, 841, 1056, 1181, 1502, 1563, 2067
TspRI	CASTG	6 cut(s)	193, 417, 707, 1336, 1615, 1782
Van91I	CCANNNNNTGG	1 cut(s)	1781
VpaK11BI	GGWCC	1 cut(s)	978
VspI	ATTAAT	2 cut(s)	47, 1301
XapI	RAATTY	2 cut(s)	950, 1550
XceI	RCATGY	2 cut(s)	1792, 1822
XmaJI	CCTAGG	1 cut(s)	2038
XmnI	GAANNNNTTC	2 cut(s)	48, 1672
XspI	CTAG	7 cut(s)	620, 902, 1409, 1427, 1677, 2015, 2039
ZraI	GACGTC	1 cut(s)	1094
Zsp2I	ATGCAT	3 cut(s)	916, 1368, 1852

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.