Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr03

Orthogroup ID

OG0010841

Physical Location & Seq

Genomic Coordinates Forward (+)

37180057 .. 37188800

Gene Structure 8744 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

MD03G1296000.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 3957 bp

ATGTCGGCGTCGCGTCGTCGGACCAACGGCCAATCCCCACTGGTCAACCCACAGCGCCAAATCACTTCCTTCTTCTCCAAAGCCACTTCCACTCCGTCCCCAATTTCTTCCAAACAAACCCAAAACCCTAAATCTACCCGCATTTCTTCCAAGCAAACCCAAAACCCTAAATCCACCCCCATTTCTTCCAAACCAACCTTAATCCCTAAATCTACCCCCATTTCTTCCAGACAAACCCTAAACCCTAATCCGAATCCTAGTCCCGGTCATAGCCCTAGCCCCACAACCCCTTCGCCTCTCGATTCCAAGCCCAGCTCCAAGACCAGCCCCAAAAAATCCCACGGCCACGAGGTTGTCGGGAAGAGGATCAAGGTTTACTGGCCGTTGGATAAGAGCTGGTACGAAGGTTATGTCAAGTGCTTCAATAAGCACAGTGGTAAGCATTTAGTTCAGTACGACGATGCTGAAGAAGAAATGTTGGATTTGGAAATGGAGAAGATCGAGTGGGTCCCGGAAACCGTGAAGATTTTCAAGCGCTTGAGGCGAGGCCCTTTGCCAACTCCCACCAATGTGGATGTAGATGTGGTGATGGAGGATGAGGATAAGGAAGACAGCAACGGTGTAGCTGATGATGATTCGAGCGATGAGGATTGGGGAAAGAGTGGGGACAAAGAGTTAGTTGCTGAGGTTGGTGACGATGAAGAGGAGCTTAGGGAATTGGAGGATGAGGAAGATGATGTGGTTCCCACGAACACAAACAAAAGAAAGCGGGGTGACTCCGGTGGTAGAAGAAAATTGAGCGGTGGAGGGAGTTTGGGGTCTGCTAAGAAGACCAAGTGTGGTGGAAATGTGGGTATGAACGGCTTCAAGGGCGTGTCACCGGAGCCTACCCCTAATGCTGAAGGGACAAAGGCGACTAATGGTATGGATACTGTTTTATCTGGTGATGCCTCAGCAAGGTTTAGCTTGCGTGAAGCTGAGAAGTTGCACTTCCTTGGGGAACAGCGCATGGATGCCAAAAGAAGAAGCCTTGGAGATGCAAATTATGATCCAAGAACTCTGTACTTACCTCCCGGATTCTTAAAGAGTTTATCAGGTGGCCAGAGACAATGGTGGGAGTTTAAGTCAAAACACATGGACAAAGTTATATTTTTCAAGATGGGCAAATTTTATGAACTTTTTGAAATGGATGCACATATAGGAGCAAAAGAACTTGGTCTGCAGTATATGAAGGGTGAACAACCCCATTGTGGATTTCCGGAGAAGAATTTCTCAATGAACGTGGAGAAACTTGCTAGGAAGGGGTATCGAGTTCTTGTAGTGGAGCAGACAGAAACACCTGAACAGATGGATCTTCGTCGTAAAAAGGATGGCTCTATAGATAAGGTTGTGAAACGTGAAATATGTGCAGTGGTTACAAAAGGAACACTAACTGAGGGAGAGATGCTGTCAGCAAACCCTGATGCTTCTTATCTAATGGCAGTGACTGAAAATTCTCAGAATTTGGCAAACGAAAATGCAGAGCGGGTTTTTGGGGTTTGTGTAGTTGATGTTGCTACCAGCCGGGTCATTCTTGGGCAGTTTGGAGATGATCTAGAGTGCAGTGCCTTGTCTTGTCTCTTGTCTGAGCTGAGGCCTGTTGAAATTGTAAAACCTGCAAATCTACTCAGTCCTGAAACAGAGAAAGTGCTGATTAGGCATACAAGAAGTCCTCTAGTGAATGAGTTAGTCCCAGTATTGGAATTCTTGGATGCTGAGAGAACTGTTCAGGAAGTTAGAAGTATATACAGGCGTGCCGATGATCAAGATGTTTCTGGTTCTCCAAAGAAAGACGGTTTTCATGGCAGTGATTCTCATTTAGAAGAAGATAGCTATGGCTGCTTGCCAGATGTGTTGTCAGAGATGGTGAGAGCAGGTGAGAATGGCATCTCTGCACTTTCAGCTCTTGGAGGTGCTCTTTTCTATTTAAAGCAGGCCTTCCTGGATGAGACAATACTCAGATTTGCAAAATTTGAGTTACTTCCAAGTTCTGGTTTTGGTGATACCGTATCAAAACCATACATGGTTCTTGACTCAGCTGCCCTAGAGAACCTTGAGATTTTTGAGAACAACAGAAATGGAGACTCTTCAGGGACATTGTATGCACAATTGAACCACTGTGTTACTGGATTTGGGAAGAGATTGCTTAGAACATGGCTTGCAAGACCTCTCTATCATGTAGAATTAATAAAGCAACGCCAGGATGCTGTTGCAAGTCTTCGGGGAGTTAATCTTCCTTATGCACTTGAATTTCATAAAGCAGTGTCTAGGCTGCCGGACATGGAGCGGTTGCTTGTGCGTGTCTTCTCTAGTAGTAATGCTTGCGGAAGGAATGCTAAGAAAGTGGTTTTATACGAGGATGCTGCAAAAAAACAGCTTCAGGAGTTTATTTCAGCTTTACGTGGTTGTGAACTCATGGCTCAAACATGTTGTTCTCTTAACGTTATCTTGGAAAATGTTGAATCTGCACAACTTCATCATCTGCTGACACTGGGTAAAGGTCTTCCTGATGTCAACTCAGTTCTAAAACATTTCAAGGATGCCTTTGATTGGGTAGAAGCCAATAATTCAGGTCGTATAATACCTCATGAAGGAGTCGATATTGAGTATGACTCTGCTTGTGAAAAAGTAAAGGAGATAGAGTCTCATTTAACAAAACACCTCCAAGAACAGAGAAAGTTACTGGGAGATAAATCGATCACTTATGTCACAGTTGGAAAAGATTCTTACCTATTGGAAATGCCAGAAAGTCTGTGCAGTTGTATTCCTCCGGATTATGAGTTACGTTCGTCCAAAAAGGGTTTCTCGAGGTACTGGACCCCAGATATCAAGAAGTCATTGACAGAGCTCTCGCAAGCTGAAACTGATAAGGAGTCCTCACTAAAAAGCATTTTGCAGAGGTTAATTGGACGTTTCTGCGAGCATCGTCTTAAATGGAGACAATTGGTCTCTGTGACTGCAGAAATCGATGTTTTAATCAGTCTAGCAATTGCAAGTGATTATTTTGAAGGACCCTCTTGTCAGCCAGTTGTCATGAGTTCATCATGTACTAATGAAGTACCTCGTTTTTCTGCAAAAAGTTTGGGCCATCCTGTTCTTAGAAGTGATTCTTTAGGAAAGGGTACATTTGTATCCAACGACATTTCCATTGGAGGTTCTGGCCATGCAAGCTTTATCCTTCTCACTGGTCCTAACATGGGTGGAAAGTCCACTCTTATTCGCCAGGTTTGCTTGGCTGTGATTTTAGCACAGTTAGGAGCAGATGTTCCTGCAGAGAGCTTTGAGCTATCTCCTGTTGACCGAATCTTTGTTCGTATGGGTGCTAAAGATAATATCATGGTAGGCCAAAGTACATTTCTGACGGAGCTTTCAGAAACTGCAACAATGCTGTCTTCAGCTACTCGCAATTCATTGGTGGCACTGGATGAACTTGGACGTGGAACATCAACTTCAGATGGACAAGCCATTGCGGAATCAGTTCTTGAACATTTTATCTACAAGGTCCAGTGCCGAGGAATGTTTTCTACCCATTATCACCGACTAGCTGTTGATTATCAAAATAATTCTAAGGTTTCACTATGTCATATGGCATGCCAAGTTGGAAACGGAGATGGAGGTGTGGAAGAAGTTACATTTCTATACAGGTTGAATCCTGGTGCATGCCCTAAAAGCTATGGTGTCAACATTGCACGGTTAGCTGGACTTCCTATTTCAGTCCTTAAAAAAGCTACCGCCAAGTCCAGGGAATTCGAGGCTACATATGGTAAACACAGGAAGGCATCTGGAGATAACATTTGCTTTCAAGAGTTGATTAGTATTGTGGCAAAATTGACGAGTCATGAGTTTACCAAAAGCATTGACATGGACTCCCTGACAGAAGTTTGGCATAGAGCAAGACTACTACAGCAAGAAAGTTGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000217 GO:0000404 GO:0003674 GO:0003676 GO:0003677 GO:0003684 GO:0003690 GO:0003824 GO:0005488 GO:0005575 GO:0005622 GO:0005623 GO:0006139 GO:0006259 GO:0006281 GO:0006290 GO:0006298 GO:0006725 GO:0006807 GO:0006950 GO:0006974 GO:0006996 GO:0008094 GO:0008150 GO:0008152 GO:0009987 GO:0016043 GO:0016462 GO:0016787 GO:0016817 GO:0016818 GO:0016887 GO:0017111 GO:0030983 GO:0032135 GO:0032300 GO:0032991 GO:0033554 GO:0034641 GO:0042623 GO:0043170 GO:0043570 GO:0044237 GO:0044238 GO:0044260 GO:0044424 GO:0044464 GO:0046483 GO:0050896 GO:0051276 GO:0051716 GO:0071704 GO:0071840 GO:0090304 GO:0097159 GO:1901360 GO:1901363 GO:1990391

KEGG Pathways

Metabolic & Signaling

ko01524 ko03430 ko05200 ko05210 map01524 map03430 map05200 map05210

Pfam Domains

Protein Families

MutS_I MutS_II MutS_III MutS_IV MutS_V

Protein Analysis

1319

Amino Acids

145.82

Weight (kDa)

6.33

Isoelectric Point (pI)

45.31

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
MutS_I	PF01624	367 - 480	2.1e-32	MutS domain I
MutS_II	PF05188	491 - 571	9e-08	MutS domain II
MutS_III	PF05192	693 - 1009	9.1e-34	MutS domain III
MutS_IV	PF05190	878 - 968	6.9e-17	MutS family domain IV
MutS_V	PF00488	1072 - 1263	7e-67	MutS domain V

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

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Align Labels (Cladogram)

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Orthologous Genes (Group: OG0010841)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT4G02070 AT4G02070
fragaria_vesca	FvH4_5g39410 FvH4_5g39410 FvH4_5g39410 FvH4_5g39410
malus_domestica	MD01G1013000.v1.1 MD03G1296000.v1.1 MD11G1315400.v1.1
prunus_persica	Prupe.8G271300_v2.0.a1 Prupe.8G271300_v2.0.a1 Prupe.8G271300_v2.0.a1 Prupe.8G271300_v2.0.a1
pyrus_communis	pycom03g23420 pycom11g27540
rosa_laevigata	RLG00000000552
rosa_multiflora	Rmu_sc0022843.1_g000001
rosa_roxburghii	Rroxscaffold_3G00218800
rosa_samantha	Rh7CG517400 Rh7DG485900

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AarI	CACCTGC	1 cut(s)	1904
AasI	GACNNNNNNGTC	1 cut(s)	3057
Acc36I	ACCTGC	2 cut(s)	1663, 1904
AccB7I	CCANNNNNTGG	1 cut(s)	2030
AccBSI	CCGCTC	3 cut(s)	801, 1525, 2326
AccII	CGCG	1 cut(s)	13
AccIII	TCCGGA	2 cut(s)	1258, 2809
AciI	CCGC	8 cut(s)	139, 769, 801, 1525, 2326, 2364, 3509, 3771
AclI	AACGTT	1 cut(s)	2481
AclWI	GGATC	3 cut(s)	374, 1043, 1359
AcoI	YGGCCR	5 cut(s)	28, 343, 380, 1099, 3199
AcsI	RAATTY	8 cut(s)	1166, 1267, 1492, 1501, 1742, 2009, 2288, 3785
AcuI	CTGAAG	6 cut(s)	486, 921, 2112, 2402, 3417, 3474
AcyI	GRCGYC	1 cut(s)	8
AfaI	GTAC	8 cut(s)	401, 455, 1064, 2852, 3088, 3099, 3163, 3391
AfeI	AGCGCT	1 cut(s)	536
AfiI	CCNNNNNNNGG	9 cut(s)	263, 957, 1250, 1738, 2030, 2589, 2630, 3236, 3780
AflIII	ACRYGT	1 cut(s)	2465
AhdI	GACNNNNNGTC	1 cut(s)	2984
AjiI	CACGTC	1 cut(s)	3476
AjnI	CCWGG	5 cut(s)	1980, 2238, 3261, 3691, 3779
AjuI	GAANNNNNNNTTGG	4 cut(s)	2825, 2857, 3378, 3410
AleI	CACNNNNGTG	1 cut(s)	569
AloI	GAACNNNNNNTCC	2 cut(s)	3288, 3320
Alw21I	GWGCWC	2 cut(s)	1957, 2889
Alw26I	GTCTC	7 cut(s)	1099, 1622, 1982, 2115, 2688, 2971, 2992
AlwI	GGATC	3 cut(s)	374, 1043, 1359
AlwNI	CAGNNNCTG	2 cut(s)	1487, 3416
Ama87I	CYCGRG	1 cut(s)	2845
Aor13HI	TCCGGA	2 cut(s)	1258, 2809
Aor51HI	AGCGCT	1 cut(s)	536
ApeKI	GCWGC	4 cut(s)	1878, 2078, 2311, 2402
ApoI	RAATTY	8 cut(s)	1166, 1267, 1492, 1501, 1742, 2009, 2288, 3785
ArsI	GACNNNNNNTTYG	4 cut(s)	396, 428, 3329, 3361
AseI	ATTAAT	1 cut(s)	2225
Asp700I	GAANNNNTTC	7 cut(s)	527, 863, 1268, 2761, 3145, 3516, 3559
AspLEI	GCGC	3 cut(s)	57, 537, 1008
AspS9I	GGNCC	8 cut(s)	21, 508, 548, 2856, 3050, 3124, 3227, 3541
AsuC2I	CCSGG	4 cut(s)	264, 512, 1074, 1565
AvaI	CYCGRG	1 cut(s)	2845
AvaII	GGWCC	6 cut(s)	21, 508, 2856, 3050, 3227, 3541
BaeI	ACNNNNGTAYC	6 cut(s)	391, 424, 3153, 3153, 3186, 3186
BalI	TGGCCA	2 cut(s)	1101, 3201
BanII	GRGCYC	1 cut(s)	2889
BauI	CACGAG	1 cut(s)	347
BbsI	GAAGAC	6 cut(s)	615, 836, 2249, 2335, 2534, 3423
Bbv12I	GWGCWC	2 cut(s)	1957, 2889
BbvCI	CCTCAGC	3 cut(s)	684, 952, 1631
BbvI	GCAGC	4 cut(s)	1865, 2065, 2298, 2389
BccI	CCATC	8 cut(s)	583, 1153, 1342, 1364, 1897, 3135, 3488, 3644
BceAI	ACGGC	4 cut(s)	43, 358, 367, 877
BcgI	CGANNNNNNTGC	2 cut(s)	2384, 2418
BciT130I	CCWGG	5 cut(s)	1982, 2240, 3263, 3693, 3781
BciVI	GTATCC	2 cut(s)	922, 3181
BclI	TGATCA	1 cut(s)	1801
BcnI	CCSGG	4 cut(s)	264, 512, 1074, 1565
BcoDI	GTCTC	7 cut(s)	1099, 1622, 1982, 2115, 2688, 2971, 2992
BfmI	CTRYAG	5 cut(s)	1220, 1377, 2997, 3309, 3941
BfoI	RGCGCY	2 cut(s)	58, 538
BfuAI	ACCTGC	2 cut(s)	1663, 1904
BfuI	GTATCC	2 cut(s)	922, 3181
BisI	GCNGC	4 cut(s)	1879, 2079, 2312, 2403
BlsI	GCNGC	4 cut(s)	1880, 2080, 2313, 2404
Bme1390I	CCNGG	9 cut(s)	264, 512, 1074, 1565, 1982, 2240, 3263, 3693, 3781
Bme18I	GGWCC	6 cut(s)	21, 508, 2856, 3050, 3227, 3541
BmeRI	GACNNNNNGTC	1 cut(s)	2984
BmeT110I	CYCGRG	1 cut(s)	2845
BmgBI	CACGTC	1 cut(s)	3476
BmgT120I	GGNCC	8 cut(s)	21, 508, 548, 2856, 3050, 3124, 3227, 3541
BmiI	GGNNCC	6 cut(s)	509, 510, 744, 885, 2858, 3052
BmrFI	CCNGG	9 cut(s)	264, 512, 1074, 1565, 1982, 2240, 3263, 3693, 3781
BmrI	ACTGGG	3 cut(s)	1727, 2540, 2732
BmuI	ACTGGG	3 cut(s)	1727, 2540, 2732
BpiI	GAAGAC	6 cut(s)	615, 836, 2249, 2335, 2534, 3423
BplI	GAGNNNNNCTC	2 cut(s)	2636, 2668
BpmI	CTGGAG	1 cut(s)	3843
Bpu10I	CCTNAGC	4 cut(s)	684, 710, 952, 1631
BpuEI	CTTGAG	2 cut(s)	559, 2114
BpuMI	CCSGG	4 cut(s)	264, 512, 1074, 1565
Bsa29I	ATCGAT	2 cut(s)	2735, 3006
BsaAI	YACGTR	1 cut(s)	2441
BsaHI	GRCGYC	1 cut(s)	8
BsaI	GGTCTC	1 cut(s)	2992
BsaJI	CCNNGG	5 cut(s)	340, 994, 1030, 3550, 3780
BsaWI	WCCGGW	4 cut(s)	779, 880, 1258, 2809
Bsc4I	CCNNNNNNNGG	9 cut(s)	263, 957, 1250, 1738, 2030, 2589, 2630, 3236, 3780
Bse3DI	GCAATG	2 cut(s)	3504, 3723
BseAI	TCCGGA	2 cut(s)	1258, 2809
BseBI	CCWGG	5 cut(s)	1982, 2240, 3263, 3693, 3781
BseCI	ATCGAT	2 cut(s)	2735, 3006
BseDI	CCNNGG	5 cut(s)	340, 994, 1030, 3550, 3780
BseLI	CCNNNNNNNGG	9 cut(s)	263, 957, 1250, 1738, 2030, 2589, 2630, 3236, 3780
BseMI	GCAATG	2 cut(s)	3504, 3723
BseRI	GAGGAG	1 cut(s)	719
BseXI	GCAGC	4 cut(s)	1865, 2065, 2298, 2389
BseYI	CCCAGC	1 cut(s)	311
BsgI	GTGCAG	5 cut(s)	1428, 1621, 1917, 2490, 2815
Bsh1236I	CGCG	1 cut(s)	13
BshVI	ATCGAT	2 cut(s)	2735, 3006
BsiHKAI	GWGCWC	2 cut(s)	1957, 2889
BsiHKCI	CYCGRG	1 cut(s)	2845
BsiSI	CCGG	9 cut(s)	264, 512, 780, 881, 1074, 1259, 1564, 2315, 2810
BslFI	GGGAC	7 cut(s)	82, 246, 494, 680, 919, 1715, 2146
BslI	CCNNNNNNNGG	9 cut(s)	263, 957, 1250, 1738, 2030, 2589, 2630, 3236, 3780
BsmAI	GTCTC	7 cut(s)	1099, 1622, 1982, 2115, 2688, 2971, 2992
BsmFI	GGGAC	7 cut(s)	82, 246, 494, 680, 919, 1715, 2146
BsmI	GAATGC	1 cut(s)	2377
Bso31I	GGTCTC	1 cut(s)	2992
BsoBI	CYCGRG	1 cut(s)	2845
Bsp1286I	GDGCHC	2 cut(s)	1957, 2889
Bsp13I	TCCGGA	2 cut(s)	1258, 2809
Bsp143I	GATC	7 cut(s)	366, 498, 1048, 1351, 1591, 1801, 2736
BspACI	CCGC	8 cut(s)	139, 769, 801, 1525, 2326, 2364, 3509, 3771
BspDI	ATCGAT	2 cut(s)	2735, 3006
BspEI	TCCGGA	2 cut(s)	1258, 2809
BspFNI	CGCG	1 cut(s)	13
BspHI	TCATGA	3 cut(s)	2626, 3072, 3877
BspLI	GGNNCC	6 cut(s)	509, 510, 744, 885, 2858, 3052
BspMAI	CTGCAG	3 cut(s)	1224, 3001, 3313
BspMI	ACCTGC	2 cut(s)	1663, 1904
BspPI	GGATC	3 cut(s)	374, 1043, 1359
BspTNI	GGTCTC	1 cut(s)	2992
BsrBI	CCGCTC	3 cut(s)	801, 1525, 2326
BsrDI	GCAATG	2 cut(s)	3504, 3723
BssECI	CCNNGG	5 cut(s)	340, 994, 1030, 3550, 3780
BssMI	GATC	7 cut(s)	366, 498, 1048, 1351, 1591, 1801, 2736
BssNI	GRCGYC	1 cut(s)	8
BssSI	CACGAG	1 cut(s)	347
BssT1I	CCWWGG	2 cut(s)	994, 1030
Bst2BI	CACGAG	1 cut(s)	347
Bst2UI	CCWGG	5 cut(s)	1982, 2240, 3263, 3693, 3781
Bst6I	CTCTTC	4 cut(s)	356, 696, 2131, 2171
BstACI	GRCGYC	1 cut(s)	8
BstBAI	YACGTR	1 cut(s)	2441
BstDSI	CCRYGG	1 cut(s)	340
BstENI	CCTNNNNNAGG	2 cut(s)	955, 2628
BstFNI	CGCG	1 cut(s)	13
BstH2I	RGCGCY	2 cut(s)	58, 538
BstHHI	GCGC	3 cut(s)	57, 537, 1008
BstKTI	GATC	7 cut(s)	369, 501, 1051, 1354, 1594, 1804, 2739
BstMAI	GTCTC	7 cut(s)	1099, 1622, 1982, 2115, 2688, 2971, 2992
BstMBI	GATC	7 cut(s)	366, 498, 1048, 1351, 1591, 1801, 2736
BstMWI	GCNNNNNNNGC	9 cut(s)	541, 870, 1696, 1878, 1940, 3207, 3267, 3708, 3734
BstNI	CCWGG	5 cut(s)	1982, 2240, 3263, 3693, 3781
BstNSI	RCATGY	3 cut(s)	2469, 3633, 3702
BstSCI	CCNGG	9 cut(s)	262, 510, 1072, 1563, 1980, 2238, 3261, 3691, 3779
BstSFI	CTRYAG	5 cut(s)	1220, 1377, 2997, 3309, 3941
BstUI	CGCG	1 cut(s)	13
BstV1I	GCAGC	4 cut(s)	1865, 2065, 2298, 2389
BstV2I	GAAGAC	6 cut(s)	615, 836, 2249, 2335, 2534, 3423
BstX2I	RGATCY	1 cut(s)	1351
BstXI	CCANNNNNNTGG	1 cut(s)	571
BstYI	RGATCY	1 cut(s)	1351
Bsu15I	ATCGAT	2 cut(s)	2735, 3006
BsuI	GTATCC	2 cut(s)	922, 3181
BsuTUI	ATCGAT	2 cut(s)	2735, 3006
BtgI	CCRYGG	1 cut(s)	340
BtgZI	GCGATG	1 cut(s)	657
BtrI	CACGTC	1 cut(s)	3476
BtsI	GCAGTG	5 cut(s)	1416, 1488, 1609, 1852, 2307
BveI	ACCTGC	2 cut(s)	1663, 1904
CaiI	CAGNNNCTG	2 cut(s)	1487, 3416
CciI	TCATGA	3 cut(s)	2626, 3072, 3877
CfoI	GCGC	3 cut(s)	57, 537, 1008
Cfr13I	GGNCC	8 cut(s)	21, 508, 548, 2856, 3050, 3124, 3227, 3541
ClaI	ATCGAT	2 cut(s)	2735, 3006
CseI	GACGC	1 cut(s)	2
Csp6I	GTAC	8 cut(s)	400, 454, 1063, 2851, 3087, 3098, 3162, 3390
CspCI	CAANNNNNGTGG	4 cut(s)	823, 858, 1263, 1298
CviQI	GTAC	8 cut(s)	400, 454, 1063, 2851, 3087, 3098, 3162, 3390
DpnI	GATC	7 cut(s)	368, 500, 1050, 1353, 1593, 1803, 2738
DpnII	GATC	7 cut(s)	366, 498, 1048, 1351, 1591, 1801, 2736
DraI	TTTAAA	1 cut(s)	1968
DrdI	GACNNNNNNGTC	1 cut(s)	3057
DriI	GACNNNNNGTC	1 cut(s)	2984
DseDI	GACNNNNNNGTC	1 cut(s)	3057
EaeI	YGGCCR	5 cut(s)	28, 343, 380, 1099, 3199
Eam1104I	CTCTTC	4 cut(s)	356, 696, 2131, 2171
Eam1105I	GACNNNNNGTC	1 cut(s)	2984
EarI	CTCTTC	4 cut(s)	356, 696, 2131, 2171
Ecl136II	GAGCTC	1 cut(s)	2887
Eco130I	CCWWGG	2 cut(s)	994, 1030
Eco147I	AGGCCT	2 cut(s)	1636, 1976
Eco24I	GRGCYC	1 cut(s)	2889
Eco31I	GGTCTC	1 cut(s)	2992
Eco32I	GATATC	1 cut(s)	2866
Eco47I	GGWCC	6 cut(s)	21, 508, 2856, 3050, 3227, 3541
Eco47III	AGCGCT	1 cut(s)	536
Eco53kI	GAGCTC	1 cut(s)	2887
Eco57I	CTGAAG	6 cut(s)	486, 921, 2112, 2402, 3417, 3474
Eco88I	CYCGRG	1 cut(s)	2845
EcoICRI	GAGCTC	1 cut(s)	2887
EcoNI	CCTNNNNNAGG	2 cut(s)	955, 2628
EcoO109I	RGGNCCY	3 cut(s)	508, 548, 3050
EcoRI	GAATTC	2 cut(s)	1742, 3785
EcoRII	CCWGG	5 cut(s)	1980, 2238, 3261, 3691, 3779
EcoRV	GATATC	1 cut(s)	2866
EcoT14I	CCWWGG	2 cut(s)	994, 1030
EcoT38I	GRGCYC	1 cut(s)	2889
ErhI	CCWWGG	2 cut(s)	994, 1030
FaqI	GGGAC	7 cut(s)	82, 246, 494, 680, 919, 1715, 2146
FauI	CCCGC	3 cut(s)	146, 762, 1518
FauNDI	CATATG	2 cut(s)	3624, 3799
FbaI	TGATCA	1 cut(s)	1801
Fnu4HI	GCNGC	4 cut(s)	1879, 2079, 2312, 2403
FriOI	GRGCYC	1 cut(s)	2889
Fsp4HI	GCNGC	4 cut(s)	1879, 2079, 2312, 2403
GlaI	GCGC	3 cut(s)	56, 536, 1007
GluI	GCNGC	4 cut(s)	1879, 2079, 2312, 2403
GsaI	CCCAGC	1 cut(s)	315
GsuI	CTGGAG	1 cut(s)	3843
HaeII	RGCGCY	2 cut(s)	58, 538
HapII	CCGG	9 cut(s)	264, 512, 780, 881, 1074, 1259, 1564, 2315, 2810
HgaI	GACGC	1 cut(s)	2
HhaI	GCGC	3 cut(s)	57, 537, 1008
Hin1I	GRCGYC	1 cut(s)	8
Hin6I	GCGC	3 cut(s)	55, 535, 1006
HinP1I	GCGC	3 cut(s)	55, 535, 1006
HincII	GTYRAC	4 cut(s)	46, 2554, 3337, 3721
HindII	GTYRAC	4 cut(s)	46, 2554, 3337, 3721
HindIII	AAGCTT	1 cut(s)	3208
HpaII	CCGG	9 cut(s)	264, 512, 780, 881, 1074, 1259, 1564, 2315, 2810
Hpy188I	TCNGA	9 cut(s)	21, 252, 1500, 1627, 1900, 2000, 3399, 3412, 3493
Hpy99I	CGWCG	5 cut(s)	13, 18, 21, 461, 1362
HpyCH4IV	ACGT	7 cut(s)	1281, 1396, 2440, 2481, 2824, 2950, 3475
HpyF10VI	GCNNNNNNNGC	9 cut(s)	541, 870, 1696, 1878, 1940, 3207, 3267, 3708, 3734
HpySE526I	ACGT	7 cut(s)	1281, 1396, 2440, 2481, 2824, 2950, 3475
Hsp92I	GRCGYC	1 cut(s)	8
HspAI	GCGC	3 cut(s)	55, 535, 1006
KflI	GGGWCCC	1 cut(s)	508
Kpn2I	TCCGGA	2 cut(s)	1258, 2809
Ksp22I	TGATCA	1 cut(s)	1801
Kzo9I	GATC	7 cut(s)	366, 498, 1048, 1351, 1591, 1801, 2736
LmnI	GCTCC	8 cut(s)	320, 706, 883, 1202, 1324, 2323, 3296, 3403
Lsp1109I	GCAGC	4 cut(s)	1865, 2065, 2298, 2389
MaeII	ACGT	7 cut(s)	1281, 1396, 2440, 2481, 2824, 2950, 3475
MalI	GATC	7 cut(s)	368, 500, 1050, 1353, 1593, 1803, 2738
MbiI	CCGCTC	3 cut(s)	801, 1525, 2326
MboI	GATC	7 cut(s)	366, 498, 1048, 1351, 1591, 1801, 2736
MfeI	CAATTG	3 cut(s)	2147, 2981, 3027
MflI	RGATCY	1 cut(s)	1351
MhlI	GDGCHC	2 cut(s)	1957, 2889
MlsI	TGGCCA	2 cut(s)	1101, 3201
MluNI	TGGCCA	2 cut(s)	1101, 3201
MlyI	GAGTC	9 cut(s)	770, 2066, 2117, 2643, 2645, 2690, 2921, 3883, 3899
MmeI	TCCRAC	5 cut(s)	366, 459, 2734, 3198, 3619
Mox20I	TGGCCA	2 cut(s)	1101, 3201
MroI	TCCGGA	2 cut(s)	1258, 2809
MroXI	GAANNNNTTC	7 cut(s)	527, 863, 1268, 2761, 3145, 3516, 3559
MscI	TGGCCA	2 cut(s)	1101, 3201
MslI	CAYNNNNRTG	3 cut(s)	569, 1845, 3899
Msp20I	TGGCCA	2 cut(s)	1101, 3201
MspA1I	CMGCKG	1 cut(s)	2078
MspI	CCGG	9 cut(s)	264, 512, 780, 881, 1074, 1259, 1564, 2315, 2810
MspR9I	CCNGG	9 cut(s)	264, 512, 1074, 1565, 1982, 2240, 3263, 3693, 3781
MunI	CAATTG	3 cut(s)	2147, 2981, 3027
Mva1269I	GAATGC	1 cut(s)	2377
MvaI	CCWGG	5 cut(s)	1982, 2240, 3263, 3693, 3781
MvnI	CGCG	1 cut(s)	13
MwoI	GCNNNNNNNGC	9 cut(s)	541, 870, 1696, 1878, 1940, 3207, 3267, 3708, 3734
NciI	CCSGG	4 cut(s)	264, 512, 1074, 1565
NdeI	CATATG	2 cut(s)	3624, 3799
NdeII	GATC	7 cut(s)	366, 498, 1048, 1351, 1591, 1801, 2736
NlaIV	GGNNCC	6 cut(s)	509, 510, 744, 885, 2858, 3052
NmeAIII	GCCGAG	1 cut(s)	3575
NmuCI	GTSAC	6 cut(s)	692, 773, 876, 1483, 2746, 2992
NspI	RCATGY	3 cut(s)	2469, 3633, 3702
OliI	CACNNNNGTG	1 cut(s)	569
PaeI	GCATGC	2 cut(s)	3633, 3702
PaeR7I	CTCGAG	1 cut(s)	2845
PagI	TCATGA	3 cut(s)	2626, 3072, 3877
PaqCI	CACCTGC	1 cut(s)	1904
PceI	AGGCCT	2 cut(s)	1636, 1976
PciI	ACATGT	1 cut(s)	2465
PctI	GAATGC	1 cut(s)	2377
PdmI	GAANNNNTTC	7 cut(s)	527, 863, 1268, 2761, 3145, 3516, 3559
PflMI	CCANNNNNTGG	1 cut(s)	2030
PfoI	TCCNGGA	3 cut(s)	510, 1072, 1980
PkrI	GCNGC	4 cut(s)	1880, 2080, 2313, 2404
PleI	GAGTC	9 cut(s)	770, 2066, 2117, 2642, 2645, 2689, 2920, 3882, 3899
PpsI	GAGTC	9 cut(s)	770, 2066, 2117, 2642, 2645, 2689, 2920, 3882, 3899
Ppu21I	YACGTR	1 cut(s)	2441
PpuMI	RGGWCCY	2 cut(s)	508, 3050
PscI	ACATGT	1 cut(s)	2465
PshBI	ATTAAT	1 cut(s)	2225
Psp124BI	GAGCTC	1 cut(s)	2889
Psp1406I	AACGTT	1 cut(s)	2481
Psp5II	RGGWCCY	2 cut(s)	508, 3050
Psp6I	CCWGG	5 cut(s)	1980, 2238, 3261, 3691, 3779
PspFI	CCCAGC	1 cut(s)	311
PspGI	CCWGG	5 cut(s)	1980, 2238, 3261, 3691, 3779
PspN4I	GGNNCC	6 cut(s)	509, 510, 744, 885, 2858, 3052
PspPI	GGNCC	8 cut(s)	21, 508, 548, 2856, 3050, 3124, 3227, 3541
PspPPI	RGGWCCY	2 cut(s)	508, 3050
PstI	CTGCAG	3 cut(s)	1224, 3001, 3313
PstNI	CAGNNNCTG	2 cut(s)	1487, 3416
PsuI	RGATCY	1 cut(s)	1351
PvuII	CAGCTG	1 cut(s)	2078
RsaI	GTAC	8 cut(s)	401, 455, 1064, 2852, 3088, 3099, 3163, 3391
RsaNI	GTAC	8 cut(s)	400, 454, 1063, 2851, 3087, 3098, 3162, 3390
RseI	CAYNNNNRTG	3 cut(s)	569, 1845, 3899
SacI	GAGCTC	1 cut(s)	2889
SatI	GCNGC	4 cut(s)	1879, 2079, 2312, 2403
Sau3AI	GATC	7 cut(s)	366, 498, 1048, 1351, 1591, 1801, 2736
Sau96I	GGNCC	8 cut(s)	21, 508, 548, 2856, 3050, 3124, 3227, 3541
SchI	GAGTC	9 cut(s)	770, 2066, 2117, 2643, 2645, 2690, 2921, 3883, 3899
ScrFI	CCNGG	9 cut(s)	264, 512, 1074, 1565, 1982, 2240, 3263, 3693, 3781
SduI	GDGCHC	2 cut(s)	1957, 2889
SfcI	CTRYAG	5 cut(s)	1220, 1377, 2997, 3309, 3941
Sfr274I	CTCGAG	1 cut(s)	2845
SinI	GGWCC	6 cut(s)	21, 508, 2856, 3050, 3227, 3541
SlaI	CTCGAG	1 cut(s)	2845
SmiMI	CAYNNNNRTG	3 cut(s)	569, 1845, 3899
SmlI	CTYRAG	3 cut(s)	538, 2093, 2845
SmoI	CTYRAG	3 cut(s)	538, 2093, 2845
SphI	GCATGC	2 cut(s)	3633, 3702
SseBI	AGGCCT	2 cut(s)	1636, 1976
SsiI	CCGC	8 cut(s)	139, 769, 801, 1525, 2326, 2364, 3509, 3771
SstI	GAGCTC	1 cut(s)	2889
StuI	AGGCCT	2 cut(s)	1636, 1976
StyD4I	CCNGG	9 cut(s)	262, 510, 1072, 1563, 1980, 2238, 3261, 3691, 3779
StyI	CCWWGG	2 cut(s)	994, 1030
TaiI	ACGT	7 cut(s)	1284, 1399, 2443, 2484, 2827, 2953, 3478
TaqI	TCGA	9 cut(s)	300, 501, 638, 1309, 2637, 2735, 2846, 3006, 3789
TaqII	GACCGA	1 cut(s)	3354
TatI	WGTACW	3 cut(s)	1062, 3086, 3389
TseFI	GTSAC	6 cut(s)	692, 773, 876, 1483, 2746, 2992
TseI	GCWGC	4 cut(s)	1878, 2078, 2311, 2402
Tsp45I	GTSAC	6 cut(s)	692, 773, 876, 1483, 2746, 2992
TspGWI	ACGGA	3 cut(s)	84, 3416, 3660
Van91I	CCANNNNNTGG	1 cut(s)	2030
VpaK11BI	GGWCC	6 cut(s)	21, 508, 2856, 3050, 3227, 3541
VspI	ATTAAT	1 cut(s)	2225
XagI	CCTNNNNNAGG	2 cut(s)	955, 2628
XapI	RAATTY	8 cut(s)	1166, 1267, 1492, 1501, 1742, 2009, 2288, 3785
XbaI	TCTAGA	1 cut(s)	1594
XceI	RCATGY	3 cut(s)	2469, 3633, 3702
XcmI	CCANNNNNNNNNTGG	1 cut(s)	2162
XhoI	CTCGAG	1 cut(s)	2845
XmnI	GAANNNNTTC	7 cut(s)	527, 863, 1268, 2761, 3145, 3516, 3559

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.