MD04G1004600.v1.1

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr04
OG0014602
Physical Location & Seq
Forward (+)
585280 .. 588205
2926 bp
Loading structure...
UTR
Exon/CDS
Intron
MD04G1004600.v1.1.491

Sequence Viewer

Length: 2301 bp
ATGGAGGTTGACGAAATGGATTCGCTCTTCGAGGGCATGGTCTTGTTTACCCCTAACAATTCTGGTAATAACAGTCTACCCCAATTGGAGGATTTGGAGAAACACCATCTCCCTGATCACGGTGGTCAAGATCCAGCCCTTCAATCTCACCAAGATGGAGATGCTCCTCCACCTCCGCCACCTCCGCCAGAATCATCATCCTCCGAGCCTCTTGACGAGAACCTCTTCTCCGACCTCACAGTCCAATTCCAGACCCTCCCAAACCCAGAAGGTCCGCTAACACAGACACACGACGACCTTGTTCAGAATCAAACCCCCGCATCGAAACCAACACCGCCTGCGACAACATCCAGACAGAACTCCTCCACTTCCAGACGGAAAAAGCGAGCCGCTGGCCTCCGCATCGGATACGCCCGCGATTCCTATTCCAGTTCCAGGGCCGATGATCCCGATCTCGACGACGACTCCCTCCCCTACACCTCCACCTCCATCTCCACCAGCCCCGTGGTCCCTACTTCCACTCCATCTTCTGACTCCACTCCCCCTCCTCCCCCGCCTCCGCCTGTATCCTTATCAGTATCGGAACCAGAACCAGTACCAGTAGAAGTGGTCGTAAATGACAAAGAAGCCCCTCCCGAGCTCCGGTTGGATCAGAGCAAGGCTCTGATCACCGACAAGCTCAGTCACGCTCGCCAATTGGCTGCCTCCATCTCCGCCGCAAGGAAGGAATCCATTGCCAAGAGAAGGGCAGCAGCGGACCATCTCAATTTGGCGTCTGTCAATGAAGCCAATCTTGAGAAACAGCTGGAAGAGGCTTGCGAGGCTGAGGATTTCGACACTGCACAAAGGCTCAGTGACAGCCTTGCAGCTGCTCAGAGCCAGAAGCAAGTTCTGTTAGCCGCCCTCAGAGATGCCGAGGCCGAATGTGATGCTGTGGACACCAAAATGCATCAAGTTCTCCAATCTCAAATCACCGCCGAGGAAGAATGTGCTTCTCTGCTTCAACGCTTTGCCACCGATGCCTCGAATGATGCAGATTTGGTCTTACAAACAGCAGAAGCCCACTCTTCAAAAGAAACAGAAGAATGGCTTTCATCGACTGAAGCCTTGCATGCCAAGAAAATGGAACTGGAGATTGAATCCCATATCATTGATGATGCTGGGCTTGTCTTGGACTCTTCCATTGACACTTTGGTGGAGGATGACAAGAGGGAGAAAGAATGTCTCTGTAAGAAAAAGGACGTTCTCATGGACGAACTGGAGAAGCTACTTGCTTTGGTGAAACTGAAGGAACAAGAGATTGCACAAAATGATCGTGACATCAAACAAGTTGAGGAAAGGATTGATCTTGCTCTTTCTGGCTTCCAGGACATGCAATCCAGCATTCAGGCCAAGTCCAGCAACTTGCAAGCGGCCCTCTCTAGGATAAATGTAGAGAGTGAGGCTCTGTCAACAAAAAAGACGGAAATTGATGCCTTCCTTACTCAGGAACAAGAGAGGGAAGCAAAGTTGAGGGAACTGGCCAGGGCTTCCACAGAGGAAGCAGAGGCATACCAACAAGTTGCTGCTCTTAGAAAAACTCTCATGTCCTCTATTTTGAAGTCCAGGGAAGATAATATCACGCTTGCAAAGACTGAGGAAGAGCTCTCCAAGGATGTTCAAATGCTCCAACAGGATGTTTCTGCCTCAAGAGCTTCACTGCAGGAACTGTCTTCGAAAAAGTCGAGCATTCAGCAAGATATAGCATCCTCTAAGCAGAAAATGATTTTCATAGACAAAAGAATTCCAGAGCTGGAAGCAGAAAAGAAAGTCGCTGCAACTGCAAGAAATTTTAAGGAAGCAGCACGACTTGCTGCCGAGGCTAAGTCGTTGAATGTTGAAAAGGAAGGGATACAGAATGACATGGAAAAAGCCATCTTAGAGCTTGAGAAACTCGAGCAAGAGATAAAAGAGACTGTTAACAGATTGCAGGAGACAGAGGGGCATATTCTCTCCAAGGAAAAAGAGCTAGCTACGGCTAGGTTTCAGAGGTTGCTTCTGATATCTGGTATTGCCAAAGCAGAAAGAGAAGCTGCTTTGGAGTTGGGTAACCTTGAAGAAGCTAACCTTCTGCTTGGGGAGGCCGAAGCAGCAGACTCTGAAGCAAATAAGCTTCAACCGTTATACGATCTCAAGGTGGAAGAGGTTGAAAGATTACCAAAGCAGTTCATCTCCGTGGAGCTTATATCTAATCTTGGGAGGAAGCAGTTAGAAGAATTAGCAGCATCGGTGCAACCTTCTCAGGGAAAGAGTTTGATATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
UVR

Protein Analysis

767

Amino Acids

84.24

Weight (kDa)

4.67

Isoelectric Point (pI)

61.2

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0014602)

Species Orthologous Gene IDs
arabidopsis_thaliana AT5G25070
fragaria_vesca FvH4_5g26850 FvH4_5g26850
malus_domestica MD04G1004600.v1.1
prunus_persica Prupe.1G004400_v2.0.a1
pyrus_communis pycom04g00470
rosa_chinensis RchiOBHm_Chr7g0217751
rosa_laevigata RLG00000002441
rosa_multiflora Rmu_sc0000202.1_g000023 Rmu_sc0006958.1_g000001
rosa_roxburghii Rroxscaffold_3G00241710
rosa_rugosa Rorug07G0171000
rosa_samantha Rh7AG311700 Rh7BG303600 Rh7CG330500 Rh7DG311400
rosa_wichuraiana Rw7G026450

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 239
AccI GTMKAC 1 cut(s) 76
AccII CGCG 1 cut(s) 417
AclWI GGATC 3 cut(s) 125, 440, 657
AcoI YGGCCR 1 cut(s) 1521
AcsI RAATTY 2 cut(s) 1782, 1828
AcuI CTGAAG 3 cut(s) 1122, 1307, 2160
AcyI GRCGYC 1 cut(s) 773
AfaI GTAC 1 cut(s) 597
AfiI CCNNNNNNNGG 9 cut(s) 88, 119, 435, 642, 720, 744, 1422, 1486, 2282
AjnI CCWGG 4 cut(s) 434, 1365, 1523, 1604
AleI CACNNNNGTG 1 cut(s) 1193
AloI GAACNNNNNNTCC 2 cut(s) 212, 244
Alw21I GWGCWC 2 cut(s) 642, 1647
Alw26I GTCTC 3 cut(s) 1229, 1946, 1967
AlwI GGATC 3 cut(s) 125, 440, 657
AlwNI CAGNNNCTG 2 cut(s) 1708, 2138
Ama87I CYCGRG 2 cut(s) 635, 1934
AoxI GGCC 7 cut(s) 394, 438, 918, 1389, 1413, 1521, 2121
ApoI RAATTY 2 cut(s) 1782, 1828
Asp700I GAANNNNTTC 1 cut(s) 224
AspS9I GGNCC 5 cut(s) 272, 438, 508, 757, 1414
AsuHPI GGTGA 4 cut(s) 140, 661, 964, 1291
AsuII TTCGAA 1 cut(s) 1715
AsuNHI GCTAGC 1 cut(s) 2008
AvaI CYCGRG 2 cut(s) 635, 1934
AvaII GGWCC 3 cut(s) 272, 508, 757
BalI TGGCCA 1 cut(s) 1523
BanII GRGCYC 2 cut(s) 642, 1647
BbsI GAAGAC 1 cut(s) 1704
Bbv12I GWGCWC 2 cut(s) 642, 1647
BbvCI CCTCAGC 1 cut(s) 825
BccI CCATC 7 cut(s) 114, 149, 497, 532, 716, 768, 1922
BceAI ACGGC 1 cut(s) 2031
BcgI CGANNNNNNTGC 2 cut(s) 911, 945
BciT130I CCWGG 4 cut(s) 436, 1367, 1525, 1606
BciVI GTATCC 3 cut(s) 401, 577, 1884
BclI TGATCA 2 cut(s) 115, 666
BcoDI GTCTC 3 cut(s) 1229, 1946, 1967
BfaI CTAG 3 cut(s) 1422, 2009, 2019
BfmI CTRYAG 1 cut(s) 1700
BfuI GTATCC 3 cut(s) 401, 577, 1884
Bme1390I CCNGG 4 cut(s) 436, 1367, 1525, 1606
Bme18I GGWCC 3 cut(s) 272, 508, 757
BmeT110I CYCGRG 2 cut(s) 635, 1934
BmgT120I GGNCC 5 cut(s) 272, 438, 508, 757, 1414
BmiI GGNNCC 2 cut(s) 510, 585
BmrFI CCNGG 4 cut(s) 436, 1367, 1525, 1606
BmtI GCTAGC 1 cut(s) 2012
BpiI GAAGAC 1 cut(s) 1704
BplI GAGNNNNNCTC 4 cut(s) 646, 678, 1429, 1461
BpmI CTGGAG 2 cut(s) 1151, 1280
Bpu10I CCTNAGC 1 cut(s) 825
Bpu14I TTCGAA 1 cut(s) 1715
BpuEI CTTGAG 4 cut(s) 815, 1672, 1946, 2156
BsaBI GATNNNNATC 1 cut(s) 450
BsaHI GRCGYC 1 cut(s) 773
BsaWI WCCGGW 1 cut(s) 642
Bsc4I CCNNNNNNNGG 9 cut(s) 88, 119, 435, 642, 720, 744, 1422, 1486, 2282
Bse1I ACTGG 6 cut(s) 429, 593, 599, 1134, 1263, 1524
Bse3DI GCAATG 1 cut(s) 732
Bse8I GATNNNNATC 1 cut(s) 450
BseBI CCWGG 4 cut(s) 436, 1367, 1525, 1606
BseGI GGATG 6 cut(s) 197, 347, 1207, 1660, 1681, 1745
BseJI GATNNNNATC 1 cut(s) 450
BseLI CCNNNNNNNGG 9 cut(s) 88, 119, 435, 642, 720, 744, 1422, 1486, 2282
BseMI GCAATG 1 cut(s) 732
BseMII CTCAG 8 cut(s) 694, 816, 865, 887, 919, 1499, 1626, 2294
BseNI ACTGG 6 cut(s) 429, 593, 599, 1134, 1263, 1524
BseRI GAGGAG 3 cut(s) 156, 352, 537
BseYI CCCAGC 1 cut(s) 1160
BsgI GTGCAG 1 cut(s) 825
Bsh1236I CGCG 1 cut(s) 417
BshFI GGCC 7 cut(s) 396, 440, 920, 1391, 1415, 1523, 2123
BsiHKAI GWGCWC 2 cut(s) 642, 1647
BsiHKCI CYCGRG 2 cut(s) 635, 1934
BsiSI CCGG 1 cut(s) 643
BslFI GGGAC 1 cut(s) 494
BslI CCNNNNNNNGG 9 cut(s) 88, 119, 435, 642, 720, 744, 1422, 1486, 2282
BsmAI GTCTC 3 cut(s) 1229, 1946, 1967
BsmFI GGGAC 1 cut(s) 494
BsmI GAATGC 2 cut(s) 1383, 1728
BsnI GGCC 7 cut(s) 396, 440, 920, 1391, 1415, 1523, 2123
BsoBI CYCGRG 2 cut(s) 635, 1934
Bsp119I TTCGAA 1 cut(s) 1715
Bsp1286I GDGCHC 2 cut(s) 642, 1647
Bsp143I GATC 9 cut(s) 115, 130, 445, 451, 649, 666, 1312, 1345, 2167
BspANI GGCC 7 cut(s) 396, 440, 920, 1391, 1415, 1523, 2123
BspCNI CTCAG 8 cut(s) 693, 817, 864, 886, 918, 1498, 1627, 2293
BspFNI CGCG 1 cut(s) 417
BspLI GGNNCC 2 cut(s) 510, 585
BspMAI CTGCAG 1 cut(s) 1704
BspOI GCTAGC 1 cut(s) 2012
BspPI GGATC 3 cut(s) 125, 440, 657
BspQI GCTCTTC 2 cut(s) 32, 1635
BspT104I TTCGAA 1 cut(s) 1715
BsrDI GCAATG 1 cut(s) 732
BsrI ACTGG 6 cut(s) 429, 593, 599, 1134, 1263, 1524
BssMI GATC 9 cut(s) 115, 130, 445, 451, 649, 666, 1312, 1345, 2167
BssNI GRCGYC 1 cut(s) 773
BssT1I CCWWGG 2 cut(s) 1650, 1995
Bst2UI CCWGG 4 cut(s) 436, 1367, 1525, 1606
Bst4CI ACNGT 6 cut(s) 74, 122, 241, 1710, 1957, 2160
Bst6I CTCTTC 7 cut(s) 32, 230, 804, 1072, 1183, 1635, 2175
BstACI GRCGYC 1 cut(s) 773
BstAPI GCANNNNNTGC 1 cut(s) 1850
BstBI TTCGAA 1 cut(s) 1715
BstDSI CCRYGG 2 cut(s) 504, 2214
BstEII GGTNACC 1 cut(s) 2087
BstENI CCTNNNNNAGG 1 cut(s) 1484
BstF5I GGATG 6 cut(s) 197, 347, 1207, 1660, 1681, 1745
BstFNI CGCG 1 cut(s) 417
BstKTI GATC 9 cut(s) 118, 133, 448, 454, 652, 669, 1315, 1348, 2170
BstMAI GTCTC 3 cut(s) 1229, 1946, 1967
BstMBI GATC 9 cut(s) 115, 130, 445, 451, 649, 666, 1312, 1345, 2167
BstMWI GCNNNNNNNGC 9 cut(s) 184, 821, 1019, 1112, 1691, 1820, 1850, 1859, 2129
BstNI CCWGG 4 cut(s) 436, 1367, 1525, 1606
BstNSI RCATGY 2 cut(s) 1115, 1375
BstPI GGTNACC 1 cut(s) 2087
BstSCI CCNGG 4 cut(s) 434, 1365, 1523, 1604
BstSFI CTRYAG 1 cut(s) 1700
BstUI CGCG 1 cut(s) 417
BstV2I GAAGAC 1 cut(s) 1704
BstX2I RGATCY 1 cut(s) 130
BstXI CCANNNNNNTGG 2 cut(s) 505, 1123
BstYI RGATCY 1 cut(s) 130
BsuI GTATCC 3 cut(s) 401, 577, 1884
BsuRI GGCC 7 cut(s) 396, 440, 920, 1391, 1415, 1523, 2123
BtgI CCRYGG 2 cut(s) 504, 2214
BtsCI GGATG 6 cut(s) 197, 347, 1207, 1660, 1681, 1745
BtsI GCAGTG 2 cut(s) 837, 1697
BtsIMutI CAGTG 3 cut(s) 837, 859, 1697
CaiI CAGNNNCTG 2 cut(s) 1708, 2138
Cfr13I GGNCC 5 cut(s) 272, 438, 508, 757, 1414
CseI GACGC 1 cut(s) 762
Csp6I GTAC 1 cut(s) 596
CviAII CATG 6 cut(s) 37, 1112, 1249, 1372, 1585, 1903
CviQI GTAC 1 cut(s) 596
DpnI GATC 9 cut(s) 117, 132, 447, 453, 651, 668, 1314, 1347, 2169
DpnII GATC 9 cut(s) 115, 130, 445, 451, 649, 666, 1312, 1345, 2167
DrdI GACNNNNNNGTC 1 cut(s) 239
DseDI GACNNNNNNGTC 1 cut(s) 239
EaeI YGGCCR 1 cut(s) 1521
Eam1104I CTCTTC 7 cut(s) 32, 230, 804, 1072, 1183, 1635, 2175
EarI CTCTTC 7 cut(s) 32, 230, 804, 1072, 1183, 1635, 2175
EciI GGCGGA 4 cut(s) 165, 174, 549, 703
Ecl136II GAGCTC 2 cut(s) 640, 1645
Eco130I CCWWGG 2 cut(s) 1650, 1995
Eco24I GRGCYC 2 cut(s) 642, 1647
Eco32I GATATC 1 cut(s) 2043
Eco47I GGWCC 3 cut(s) 272, 508, 757
Eco53kI GAGCTC 2 cut(s) 640, 1645
Eco57I CTGAAG 3 cut(s) 1122, 1307, 2160
Eco88I CYCGRG 2 cut(s) 635, 1934
Eco91I GGTNACC 1 cut(s) 2087
EcoICRI GAGCTC 2 cut(s) 640, 1645
EcoNI CCTNNNNNAGG 1 cut(s) 1484
EcoO65I GGTNACC 1 cut(s) 2087
EcoRI GAATTC 1 cut(s) 1782
EcoRII CCWGG 4 cut(s) 434, 1365, 1523, 1604
EcoRV GATATC 1 cut(s) 2043
EcoT14I CCWWGG 2 cut(s) 1650, 1995
EcoT22I ATGCAT 1 cut(s) 951
EcoT38I GRGCYC 2 cut(s) 642, 1647
ErhI CCWWGG 2 cut(s) 1650, 1995
FaeI CATG 6 cut(s) 40, 1115, 1252, 1375, 1588, 1906
FalI AAGNNNNNCTT 6 cut(s) 777, 809, 1074, 1106, 2091, 2123
FaqI GGGAC 1 cut(s) 494
FatI CATG 6 cut(s) 36, 1111, 1248, 1371, 1584, 1902
FauI CCCGC 3 cut(s) 325, 422, 561
FbaI TGATCA 2 cut(s) 115, 666
FblI GTMKAC 1 cut(s) 76
FokI GGATG 6 cut(s) 184, 334, 1214, 1667, 1688, 1732
FriOI GRGCYC 2 cut(s) 642, 1647
FspBI CTAG 3 cut(s) 1422, 2009, 2019
GsaI CCCAGC 1 cut(s) 1164
GsuI CTGGAG 2 cut(s) 1151, 1280
HaeIII GGCC 7 cut(s) 396, 440, 920, 1391, 1415, 1523, 2123
HapII CCGG 1 cut(s) 643
HgaI GACGC 1 cut(s) 762
Hin1I GRCGYC 1 cut(s) 773
Hin1II CATG 6 cut(s) 40, 1115, 1252, 1375, 1588, 1906
HincII GTYRAC 3 cut(s) 10, 1452, 1960
HindII GTYRAC 3 cut(s) 10, 1452, 1960
HindIII AAGCTT 1 cut(s) 2150
HpaI GTTAAC 1 cut(s) 1960
HpaII CCGG 1 cut(s) 643
HphI GGTGA 4 cut(s) 140, 661, 964, 1291
Hpy166II GTNNAC 6 cut(s) 10, 48, 77, 937, 1452, 1960
Hpy8I GTNNAC 6 cut(s) 10, 48, 77, 937, 1452, 1960
Hpy99I CGWCG 3 cut(s) 296, 461, 464
HpyAV CCTTC 9 cut(s) 149, 263, 718, 738, 1282, 1486, 1880, 2117, 2286
HpyCH4III ACNGT 6 cut(s) 74, 122, 241, 1710, 1957, 2160
HpyCH4IV ACGT 1 cut(s) 1242
HpyF10VI GCNNNNNNNGC 9 cut(s) 184, 821, 1019, 1112, 1691, 1820, 1850, 1859, 2129
HpySE526I ACGT 1 cut(s) 1242
Hsp92I GRCGYC 1 cut(s) 773
Hsp92II CATG 6 cut(s) 40, 1115, 1252, 1375, 1588, 1906
Ksp22I TGATCA 2 cut(s) 115, 666
KspAI GTTAAC 1 cut(s) 1960
Kzo9I GATC 9 cut(s) 115, 130, 445, 451, 649, 666, 1312, 1345, 2167
LguI GCTCTTC 2 cut(s) 32, 1635
LmnI GCTCC 4 cut(s) 169, 645, 1671, 2218
MaeI CTAG 3 cut(s) 1422, 2009, 2019
MaeII ACGT 1 cut(s) 1242
MaeIII GTNAC 4 cut(s) 683, 854, 1316, 2087
MalI GATC 9 cut(s) 117, 132, 447, 453, 651, 668, 1314, 1347, 2169
MboI GATC 9 cut(s) 115, 130, 445, 451, 649, 666, 1312, 1345, 2167
MfeI CAATTG 2 cut(s) 83, 695
MflI RGATCY 1 cut(s) 130
MhlI GDGCHC 2 cut(s) 642, 1647
MlsI TGGCCA 1 cut(s) 1523
MluCI AATT 9 cut(s) 58, 83, 245, 695, 766, 1467, 1782, 1828, 2255
MluNI TGGCCA 1 cut(s) 1523
MlyI GAGTC 4 cut(s) 458, 527, 1169, 2129
MmeI TCCRAC 3 cut(s) 255, 627, 1693
Mox20I TGGCCA 1 cut(s) 1523
Mph1103I ATGCAT 1 cut(s) 951
MroXI GAANNNNTTC 1 cut(s) 224
MscI TGGCCA 1 cut(s) 1523
MseI TTAA 2 cut(s) 1833, 1959
MslI CAYNNNNRTG 4 cut(s) 153, 944, 1193, 2213
Msp20I TGGCCA 1 cut(s) 1523
MspA1I CMGCKG 4 cut(s) 392, 755, 805, 869
MspI CCGG 1 cut(s) 643
MspR9I CCNGG 4 cut(s) 436, 1367, 1525, 1606
MunI CAATTG 2 cut(s) 83, 695
Mva1269I GAATGC 2 cut(s) 1383, 1728
MvaI CCWGG 4 cut(s) 436, 1367, 1525, 1606
MvnI CGCG 1 cut(s) 417
MwoI GCNNNNNNNGC 9 cut(s) 184, 821, 1019, 1112, 1691, 1820, 1850, 1859, 2129
NdeII GATC 9 cut(s) 115, 130, 445, 451, 649, 666, 1312, 1345, 2167
NheI GCTAGC 1 cut(s) 2008
NlaIII CATG 6 cut(s) 40, 1115, 1252, 1375, 1588, 1906
NlaIV GGNNCC 2 cut(s) 510, 585
NmeAIII GCCGAG 3 cut(s) 940, 1003, 1882
NmuCI GTSAC 3 cut(s) 683, 854, 1316
NsiI ATGCAT 1 cut(s) 951
NspI RCATGY 2 cut(s) 1115, 1375
NspV TTCGAA 1 cut(s) 1715
OliI CACNNNNGTG 1 cut(s) 1193
PaeI GCATGC 1 cut(s) 1115
PaeR7I CTCGAG 1 cut(s) 1934
PciSI GCTCTTC 2 cut(s) 32, 1635
PcsI WCGNNNNNNNCGW 2 cut(s) 382, 1721
PctI GAATGC 2 cut(s) 1383, 1728
PdmI GAANNNNTTC 1 cut(s) 224
PfeI GAWTC 6 cut(s) 20, 191, 307, 419, 728, 1139
PfoI TCCNGGA 1 cut(s) 1365
PleI GAGTC 4 cut(s) 458, 527, 1169, 2129
PpsI GAGTC 4 cut(s) 458, 527, 1169, 2129
Psp124BI GAGCTC 2 cut(s) 642, 1647
Psp6I CCWGG 4 cut(s) 434, 1365, 1523, 1604
PspEI GGTNACC 1 cut(s) 2087
PspFI CCCAGC 1 cut(s) 1160
PspGI CCWGG 4 cut(s) 434, 1365, 1523, 1604
PspN4I GGNNCC 2 cut(s) 510, 585
PspPI GGNCC 5 cut(s) 272, 438, 508, 757, 1414
PspXI VCTCGAGB 1 cut(s) 1934
PstI CTGCAG 1 cut(s) 1704
PstNI CAGNNNCTG 2 cut(s) 1708, 2138
PsuI RGATCY 1 cut(s) 130
PvuII CAGCTG 2 cut(s) 805, 869
RsaI GTAC 1 cut(s) 597
RsaNI GTAC 1 cut(s) 596
RseI CAYNNNNRTG 4 cut(s) 153, 944, 1193, 2213
SacI GAGCTC 2 cut(s) 642, 1647
SapI GCTCTTC 2 cut(s) 32, 1635
SaqAI TTAA 2 cut(s) 1833, 1959
Sau3AI GATC 9 cut(s) 115, 130, 445, 451, 649, 666, 1312, 1345, 2167
Sau96I GGNCC 5 cut(s) 272, 438, 508, 757, 1414
SchI GAGTC 4 cut(s) 458, 527, 1169, 2129
ScrFI CCNGG 4 cut(s) 436, 1367, 1525, 1606
SduI GDGCHC 2 cut(s) 642, 1647
SfcI CTRYAG 1 cut(s) 1700
Sfr274I CTCGAG 1 cut(s) 1934
SfuI TTCGAA 1 cut(s) 1715
SinI GGWCC 3 cut(s) 272, 508, 757
SlaI CTCGAG 1 cut(s) 1934
SmiMI CAYNNNNRTG 4 cut(s) 153, 944, 1193, 2213
SmlI CTYRAG 5 cut(s) 794, 1687, 1925, 1934, 2171
SmoI CTYRAG 5 cut(s) 794, 1687, 1925, 1934, 2171
SphI GCATGC 1 cut(s) 1115
Sse9I AATT 9 cut(s) 58, 83, 245, 695, 766, 1467, 1782, 1828, 2255
SspMI CTAG 3 cut(s) 1422, 2009, 2019
SstI GAGCTC 2 cut(s) 642, 1647
StyD4I CCNGG 4 cut(s) 434, 1365, 1523, 1604
StyI CCWWGG 2 cut(s) 1650, 1995
TaaI ACNGT 6 cut(s) 74, 122, 241, 1710, 1957, 2160
TaiI ACGT 1 cut(s) 1245
TaqI TCGA 9 cut(s) 30, 323, 456, 834, 1025, 1097, 1715, 1724, 1935
TasI AATT 9 cut(s) 58, 83, 245, 695, 766, 1467, 1782, 1828, 2255
TauI GCSGC 4 cut(s) 392, 719, 902, 1415
TfiI GAWTC 6 cut(s) 20, 191, 307, 419, 728, 1139
Tru1I TTAA 2 cut(s) 1833, 1959
Tru9I TTAA 2 cut(s) 1833, 1959
TscAI CASTG 3 cut(s) 844, 859, 1704
TseFI GTSAC 3 cut(s) 683, 854, 1316
Tsp45I GTSAC 3 cut(s) 683, 854, 1316
TspDTI ATGAA 4 cut(s) 798, 1083, 1759, 2197
TspGWI ACGGA 3 cut(s) 391, 1478, 2203
TspRI CASTG 3 cut(s) 844, 859, 1704
VpaK11BI GGWCC 3 cut(s) 272, 508, 757
XagI CCTNNNNNAGG 1 cut(s) 1484
XapI RAATTY 2 cut(s) 1782, 1828
XceI RCATGY 2 cut(s) 1115, 1375
XcmI CCANNNNNNNNNTGG 2 cut(s) 502, 1189
XhoI CTCGAG 1 cut(s) 1934
XmiI GTMKAC 1 cut(s) 76
XmnI GAANNNNTTC 1 cut(s) 224
XspI CTAG 3 cut(s) 1422, 2009, 2019
Zsp2I ATGCAT 1 cut(s) 951
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.