MD04G1009000.v1.1

ethylene-responsive transcription factor

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr04
OG0016683
Physical Location & Seq
Reverse (-)
1044443 .. 1045735
1293 bp
Loading structure...
UTR
Exon/CDS
Intron
MD04G1009000.v1.1.491

Sequence Viewer

Length: 1293 bp
ATGGAGGAAAAGTTTCCCAAGATGGAAACCTTCATAGAAAAGCAATTGCCATACTCTTATTTTACAAGAATGGCAACTAGTAACAATATTGGATCAAATTTGTTCGGTGATCATCCAGCCAGCTGGGGCGGCAGTGGATCAAAATCGCCACTGTCATCGGAGTCTAGTAGCTCTTCCGCAGAGGATGTTTCTCTTTCAGGTTCCAATTTGATCAACAACACAAGTAGTCCTTTTGTTCCTCTAAATTTCTTGGAAACATTTCCACAGCTGACTAGAGCTCAAGTTTCTGATCAACCACCTACTTCTCCACCTTCCTTGTCATCAAAATCATCGAGATTTCCGAAATTAACTTTGTTTTTGCAGCAACCAAGTCTGTTCGATCCATCATCATCATCATCCCCATCGTCATCGTCGTCCATGCAAGTTGAAGTTGTAAACTCACTTGGCAAGACCAAAGGGTGTAATGAGTTATCCTCTTTATTAACCCCTTCATTTCCTGCTCCCCAAGCAATTGATCAAATTCAGCATCAGCCGGGCATGATTGAGTGGCTTAAAATTAATCAAACTATAGTAAACCACTCATCCAAAGGCTCCAATGATTACTGGCTAAGCACGACGAAGACGCAGCCAATGAAGCACACTGGAAGATCATCATCAAGATTGCATCAGCAGCAGATCAATCAACAACAAAAGCAGCCTAATAACAATTTGTCCTCCTCATCATCATCACAAGGAAAGCTATTTAGAGGAGTTAGGCAGAGGCATTGGGGGAAATGGGTGGCTGAGATTCGATTACCAAGAAACCGAACAAGGGTTTGGTTGGGGACTTTTGACACCGCTGAGGAGGCTGCCATTGCATATGACACCGCGGCTTATATATTGCGCGGCGAACATGCAAAGTTGAATCTCCCAGGTTTGAAGCATCAACTTAAGGCCAGTGCTTTGAAAGGAACCACAGCTGCTCTTCTTGAAGCAAAGCTGCAAGCAATATCATCATCACAAGCACATAAGAAGGCCATCAAGGAGTCCTCTTCGTCCGAATCAGCATCATCAAACAAGCACTCAGCGGGTGATGGCGGCGATCACAAGAATATCATGATCAATAGTAATTTGAGCCAAAACCCTACAAGAAAAGAGTGGCAGTTTGGGTTGGAGAGCAAAGTTGGGTCTCATCACCATCACCACCAGCAGGATTTAGTATCAACAGCAGATGTGGATGCTGTTCAATTAAGTAGAATGCCCTCCTTGGACATGGACATGATATGGGATGCTCTTCTGGTCTCTGATTCCTAA

Protein Analysis

431

Amino Acids

47.13

Weight (kDa)

9.2

Isoelectric Point (pI)

57.7

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
AP2 PF00847 248 - 296 4.7e-13 AP2 domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0016683)

Species Orthologous Gene IDs
arabidopsis_thaliana AT4G13620
malus_domestica MD04G1009000.v1.1
prunus_persica Prupe.1G008400_v2.0.a1
pyrus_communis pycom04g00700
rosa_chinensis RchiOBHm_Chr4g0415231
rosa_laevigata RLG00000008088
rosa_multiflora Rmu_sc0001421.1_g000007
rosa_roxburghii Rroxscaffold_5G00359040
rosa_rugosa Rorug04G0132500
rosa_samantha Rh4AG193100 Rh4BG191500 Rh4CG204200 Rh4DG191000
rosa_wichuraiana Rw4G016420

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccII CGCG 2 cut(s) 869, 885
AciI CCGC 8 cut(s) 129, 177, 837, 867, 869, 885, 1067, 1077
AclWI GGATC 3 cut(s) 100, 145, 374
AcsI RAATTY 3 cut(s) 97, 244, 519
AfiI CCNNNNNNNGG 2 cut(s) 811, 1189
AflII CTTAAG 1 cut(s) 929
AgsI TTSAA 6 cut(s) 428, 904, 919, 946, 971, 1226
AhlI ACTAGT 1 cut(s) 77
AjnI CCWGG 1 cut(s) 910
AjuI GAANNNNNNNTTGG 2 cut(s) 799, 831
AluBI AGCT 7 cut(s) 123, 171, 268, 278, 739, 959, 979
AluI AGCT 7 cut(s) 123, 171, 268, 278, 739, 959, 979
Alw21I GWGCWC 1 cut(s) 280
Alw26I GTCTC 2 cut(s) 1173, 1285
AlwI GGATC 3 cut(s) 100, 145, 374
AoxI GGCC 2 cut(s) 933, 1014
ApeKI GCWGC 7 cut(s) 361, 625, 670, 694, 848, 959, 979
ApoI RAATTY 3 cut(s) 97, 244, 519
AseI ATTAAT 1 cut(s) 558
Asp700I GAANNNNTTC 2 cut(s) 12, 258
AspLEI GCGC 1 cut(s) 885
AsuC2I CCSGG 1 cut(s) 534
AsuHPI GGTGA 4 cut(s) 119, 1082, 1166, 1172
BanII GRGCYC 1 cut(s) 280
BbsI GAAGAC 1 cut(s) 626
Bbv12I GWGCWC 1 cut(s) 280
BbvCI CCTCAGC 1 cut(s) 840
BbvI GCAGC 7 cut(s) 373, 637, 682, 706, 835, 946, 966
BccI CCATC 6 cut(s) 16, 391, 409, 1025, 1067, 1185
BciT130I CCWGG 1 cut(s) 912
BclI TGATCA 5 cut(s) 109, 210, 289, 514, 1098
BcnI CCSGG 1 cut(s) 534
BcoDI GTCTC 2 cut(s) 1173, 1285
BcuI ACTAGT 1 cut(s) 77
BfaI CTAG 3 cut(s) 78, 165, 273
BfmI CTRYAG 1 cut(s) 567
BfrI CTTAAG 1 cut(s) 929
BlpI GCTNAGC 1 cut(s) 608
Bme1390I CCNGG 2 cut(s) 534, 912
BmiI GGNNCC 3 cut(s) 202, 592, 952
BmrFI CCNGG 2 cut(s) 534, 912
BmsI GCATC 6 cut(s) 535, 673, 931, 1055, 1207, 1258
BpiI GAAGAC 1 cut(s) 626
BplI GAGNNNNNCTC 2 cut(s) 458, 490
Bpu10I CCTNAGC 1 cut(s) 840
Bpu1102I GCTNAGC 1 cut(s) 608
BpuEI CTTGAG 1 cut(s) 264
BpuMI CCSGG 1 cut(s) 534
BsaBI GATNNNNATC 2 cut(s) 142, 652
BsaI GGTCTC 2 cut(s) 1173, 1285
BsaJI CCNNGG 3 cut(s) 867, 910, 1245
BsaXI ACNNNNNCTCC 1 cut(s) 26
Bsc4I CCNNNNNNNGG 2 cut(s) 811, 1189
Bse1I ACTGG 3 cut(s) 608, 646, 936
Bse3DI GCAATG 1 cut(s) 852
Bse8I GATNNNNATC 2 cut(s) 142, 652
BseBI CCWGG 1 cut(s) 912
BseDI CCNNGG 3 cut(s) 867, 910, 1245
BseGI GGATG 6 cut(s) 112, 190, 395, 581, 1222, 1273
BseJI GATNNNNATC 2 cut(s) 142, 652
BseLI CCNNNNNNNGG 2 cut(s) 811, 1189
BseMI GCAATG 1 cut(s) 852
BseMII CTCAG 3 cut(s) 774, 831, 1077
BseNI ACTGG 3 cut(s) 608, 646, 936
BseRI GAGGAG 3 cut(s) 706, 762, 857
BseXI GCAGC 7 cut(s) 373, 637, 682, 706, 835, 946, 966
BseYI CCCAGC 1 cut(s) 123
Bsh1236I CGCG 2 cut(s) 869, 885
BshFI GGCC 2 cut(s) 935, 1016
BsiHKAI GWGCWC 1 cut(s) 280
BsiSI CCGG 1 cut(s) 533
BslFI GGGAC 1 cut(s) 838
BslI CCNNNNNNNGG 2 cut(s) 811, 1189
BsmAI GTCTC 2 cut(s) 1173, 1285
BsmFI GGGAC 1 cut(s) 838
BsmI GAATGC 1 cut(s) 1242
BsnI GGCC 2 cut(s) 935, 1016
Bso31I GGTCTC 2 cut(s) 1173, 1285
Bsp1286I GDGCHC 1 cut(s) 280
Bsp1720I GCTNAGC 1 cut(s) 608
BspACI CCGC 8 cut(s) 129, 177, 837, 867, 869, 885, 1067, 1077
BspANI GGCC 2 cut(s) 935, 1016
BspCNI CTCAG 3 cut(s) 775, 832, 1076
BspFNI CGCG 2 cut(s) 869, 885
BspHI TCATGA 1 cut(s) 1095
BspLI GGNNCC 3 cut(s) 202, 592, 952
BspPI GGATC 3 cut(s) 100, 145, 374
BspQI GCTCTTC 3 cut(s) 178, 969, 1278
BspTI CTTAAG 1 cut(s) 929
BspTNI GGTCTC 2 cut(s) 1173, 1285
BsrDI GCAATG 1 cut(s) 852
BsrI ACTGG 3 cut(s) 608, 646, 936
BssECI CCNNGG 3 cut(s) 867, 910, 1245
BssT1I CCWWGG 1 cut(s) 1245
Bst2UI CCWGG 1 cut(s) 912
Bst4CI ACNGT 1 cut(s) 153
Bst6I CTCTTC 4 cut(s) 178, 969, 1036, 1278
BstAFI CTTAAG 1 cut(s) 929
BstC8I GCNNGC 2 cut(s) 121, 984
BstDEI CTNAG 4 cut(s) 608, 783, 840, 1063
BstDSI CCRYGG 1 cut(s) 867
BstF5I GGATG 6 cut(s) 112, 190, 395, 581, 1222, 1273
BstFNI CGCG 2 cut(s) 869, 885
BstHHI GCGC 1 cut(s) 885
BstMAI GTCTC 2 cut(s) 1173, 1285
BstMWI GCNNNNNNNGC 6 cut(s) 129, 506, 634, 670, 845, 854
BstNI CCWGG 1 cut(s) 912
BstNSI RCATGY 1 cut(s) 896
BstSCI CCNGG 2 cut(s) 532, 910
BstSFI CTRYAG 1 cut(s) 567
BstUI CGCG 2 cut(s) 869, 885
BstV1I GCAGC 7 cut(s) 373, 637, 682, 706, 835, 946, 966
BstV2I GAAGAC 1 cut(s) 626
BstXI CCANNNNNNTGG 1 cut(s) 123
BsuRI GGCC 2 cut(s) 935, 1016
BtgI CCRYGG 1 cut(s) 867
BtsCI GGATG 6 cut(s) 112, 190, 395, 581, 1222, 1273
BtsI GCAGTG 1 cut(s) 139
BtsIMutI CAGTG 4 cut(s) 139, 149, 639, 943
Cac8I GCNNGC 2 cut(s) 121, 984
CciI TCATGA 1 cut(s) 1095
CfoI GCGC 1 cut(s) 885
Cfr42I CCGCGG 1 cut(s) 870
CseI GACGC 1 cut(s) 631
CspCI CAANNNNNGTGG 2 cut(s) 297, 332
CviAII CATG 6 cut(s) 418, 538, 893, 1096, 1252, 1258
DdeI CTNAG 4 cut(s) 608, 783, 840, 1063
Eam1104I CTCTTC 4 cut(s) 178, 969, 1036, 1278
EarI CTCTTC 4 cut(s) 178, 969, 1036, 1278
Ecl136II GAGCTC 1 cut(s) 278
Eco130I CCWWGG 1 cut(s) 1245
Eco24I GRGCYC 1 cut(s) 280
Eco31I GGTCTC 2 cut(s) 1173, 1285
Eco53kI GAGCTC 1 cut(s) 278
EcoICRI GAGCTC 1 cut(s) 278
EcoRII CCWGG 1 cut(s) 910
EcoT14I CCWWGG 1 cut(s) 1245
EcoT38I GRGCYC 1 cut(s) 280
ErhI CCWWGG 1 cut(s) 1245
FaeI CATG 6 cut(s) 421, 541, 896, 1099, 1255, 1261
FalI AAGNNNNNCTT 2 cut(s) 214, 246
FaqI GGGAC 1 cut(s) 838
FatI CATG 6 cut(s) 417, 537, 892, 1095, 1251, 1257
FauI CCCGC 1 cut(s) 1060
FauNDI CATATG 1 cut(s) 859
FbaI TGATCA 5 cut(s) 109, 210, 289, 514, 1098
FokI GGATG 6 cut(s) 99, 197, 382, 568, 1229, 1280
FriOI GRGCYC 1 cut(s) 280
FspBI CTAG 3 cut(s) 78, 165, 273
GlaI GCGC 1 cut(s) 884
GsaI CCCAGC 1 cut(s) 127
HaeIII GGCC 2 cut(s) 935, 1016
HapII CCGG 1 cut(s) 533
HgaI GACGC 1 cut(s) 631
HhaI GCGC 1 cut(s) 885
Hin1II CATG 6 cut(s) 421, 541, 896, 1099, 1255, 1261
Hin6I GCGC 1 cut(s) 883
HinP1I GCGC 1 cut(s) 883
HinfI GANTC 6 cut(s) 161, 787, 904, 1025, 1040, 1286
HpaII CCGG 1 cut(s) 533
HphI GGTGA 4 cut(s) 119, 1082, 1166, 1172
Hpy166II GTNNAC 2 cut(s) 436, 574
Hpy188I TCNGA 5 cut(s) 160, 289, 342, 1039, 1285
Hpy188III TCNNGA 4 cut(s) 333, 657, 968, 1096
Hpy8I GTNNAC 2 cut(s) 436, 574
Hpy99I CGWCG 2 cut(s) 415, 619
HpyAV CCTTC 4 cut(s) 40, 321, 498, 1006
HpyCH4III ACNGT 1 cut(s) 153
HpyCH4V TGCA 6 cut(s) 361, 421, 664, 857, 896, 982
HpyF10VI GCNNNNNNNGC 6 cut(s) 129, 506, 634, 670, 845, 854
HpyF3I CTNAG 4 cut(s) 608, 783, 840, 1063
Hsp92II CATG 6 cut(s) 421, 541, 896, 1099, 1255, 1261
HspAI GCGC 1 cut(s) 883
Ksp22I TGATCA 5 cut(s) 109, 210, 289, 514, 1098
KspI CCGCGG 1 cut(s) 870
LguI GCTCTTC 3 cut(s) 178, 969, 1278
LmnI GCTCC 2 cut(s) 505, 596
Lsp1109I GCAGC 7 cut(s) 373, 637, 682, 706, 835, 946, 966
LweI GCATC 6 cut(s) 535, 673, 931, 1055, 1207, 1258
MaeI CTAG 3 cut(s) 78, 165, 273
MaeIII GTNAC 1 cut(s) 80
MboII GAAGA 6 cut(s) 165, 631, 657, 956, 1023, 1265
MfeI CAATTG 2 cut(s) 44, 510
MhlI GDGCHC 1 cut(s) 280
MlyI GAGTC 2 cut(s) 170, 1034
MmeI TCCRAC 1 cut(s) 1131
MroXI GAANNNNTTC 2 cut(s) 12, 258
MseI TTAA 6 cut(s) 347, 482, 552, 558, 930, 1229
MslI CAYNNNNRTG 1 cut(s) 1256
MspA1I CMGCKG 6 cut(s) 123, 268, 839, 869, 959, 1067
MspCI CTTAAG 1 cut(s) 929
MspI CCGG 1 cut(s) 533
MspR9I CCNGG 2 cut(s) 534, 912
MunI CAATTG 2 cut(s) 44, 510
Mva1269I GAATGC 1 cut(s) 1242
MvaI CCWGG 1 cut(s) 912
MvnI CGCG 2 cut(s) 869, 885
MwoI GCNNNNNNNGC 6 cut(s) 129, 506, 634, 670, 845, 854
NciI CCSGG 1 cut(s) 534
NdeI CATATG 1 cut(s) 859
NlaIII CATG 6 cut(s) 421, 541, 896, 1099, 1255, 1261
NlaIV GGNNCC 3 cut(s) 202, 592, 952
NspI RCATGY 1 cut(s) 896
PagI TCATGA 1 cut(s) 1095
PciSI GCTCTTC 3 cut(s) 178, 969, 1278
PcsI WCGNNNNNNNCGW 2 cut(s) 338, 410
PctI GAATGC 1 cut(s) 1242
PdmI GAANNNNTTC 2 cut(s) 12, 258
PfeI GAWTC 4 cut(s) 787, 904, 1040, 1286
PleI GAGTC 2 cut(s) 169, 1033
PpsI GAGTC 2 cut(s) 169, 1033
PshBI ATTAAT 1 cut(s) 558
Psp124BI GAGCTC 1 cut(s) 280
Psp6I CCWGG 1 cut(s) 910
PspFI CCCAGC 1 cut(s) 123
PspGI CCWGG 1 cut(s) 910
PspN4I GGNNCC 3 cut(s) 202, 592, 952
PvuII CAGCTG 3 cut(s) 123, 268, 959
RseI CAYNNNNRTG 1 cut(s) 1256
SacI GAGCTC 1 cut(s) 280
SacII CCGCGG 1 cut(s) 870
SapI GCTCTTC 3 cut(s) 178, 969, 1278
SaqAI TTAA 6 cut(s) 347, 482, 552, 558, 930, 1229
SchI GAGTC 2 cut(s) 170, 1034
ScrFI CCNGG 2 cut(s) 534, 912
SduI GDGCHC 1 cut(s) 280
SfaNI GCATC 6 cut(s) 535, 673, 931, 1055, 1207, 1258
SfcI CTRYAG 1 cut(s) 567
Sfr303I CCGCGG 1 cut(s) 870
SgrBI CCGCGG 1 cut(s) 870
SmiMI CAYNNNNRTG 1 cut(s) 1256
SmlI CTYRAG 2 cut(s) 279, 929
SmoI CTYRAG 2 cut(s) 279, 929
SpeI ACTAGT 1 cut(s) 77
SsiI CCGC 8 cut(s) 129, 177, 837, 867, 869, 885, 1067, 1077
SspI AATATT 1 cut(s) 88
SspMI CTAG 3 cut(s) 78, 165, 273
SstI GAGCTC 1 cut(s) 280
StyD4I CCNGG 2 cut(s) 532, 910
StyI CCWWGG 1 cut(s) 1245
TaaI ACNGT 1 cut(s) 153
TaqI TCGA 3 cut(s) 332, 378, 790
TauI GCSGC 4 cut(s) 132, 872, 888, 1080
TfiI GAWTC 4 cut(s) 787, 904, 1040, 1286
Tru1I TTAA 6 cut(s) 347, 482, 552, 558, 930, 1229
Tru9I TTAA 6 cut(s) 347, 482, 552, 558, 930, 1229
TscAI CASTG 4 cut(s) 139, 156, 646, 943
TseI GCWGC 7 cut(s) 361, 625, 670, 694, 848, 959, 979
TspDTI ATGAA 3 cut(s) 22, 480, 647
TspRI CASTG 4 cut(s) 139, 156, 646, 943
Vha464I CTTAAG 1 cut(s) 929
VspI ATTAAT 1 cut(s) 558
XapI RAATTY 3 cut(s) 97, 244, 519
XceI RCATGY 1 cut(s) 896
XmnI GAANNNNTTC 2 cut(s) 12, 258
XspI CTAG 3 cut(s) 78, 165, 273
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.