RLG00000008088

ethylene-responsive transcription factor

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr2
OG0016683
Physical Location & Seq
Reverse (-)
25459168 .. 25460355
1188 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000008088

Sequence Viewer

Length: 1188 bp
ATGGAAACCTTCATCCCAACGCAGTTGCCATGTTTTCCAGGAATGGCAAGTAGTACGGGATCGAATTTTCTCGGTGATCCGGCCAGTTGGGAAGTCGGTGGATTAAAATCTTCACTGTCACCAGAGTCTAGCACTTCTTCTTCAGCAGATGAGGTTTCTCGTAACTCCACTTTGATAAACAAAACAAATCCATTTGTTCCTGTGAATTTCCTTGAAACATTTCCTGTGCTTAACAAAGCTCATGTTTCTGATCAACCACCTACTTCTCCAGCTTCCTTGTCATCAAAGCCATCAAATTTCCCAAATTTAACTCTATTTTTGCAGGAACCAACAACGCTTTTAGATCCATCATCAACCAAAAAGTGTGATGAGTCCACGTTCTCCTCTTTAAGCCCTTCATTTCCTATACCTCACCTTGAGCACTGTCAACCAGGCTTTGATCAATGGCTCAAAATCAGTCAAACTCTAGCAAACAATCCATCTAAAGGGTTCAATGATTACTGGCTAAGTGCGACGAAAACACAGCCAATGAAGTACAGTGGAAGATCAAGATTTCAGAATCAACAACAGAAGCAACCATCAGCACCAATCCCATCGTCACAAGGAAAGCTATTTAGAGGAGTGAGGCAGAGGCATTGGGGAAAATGGGTGGCTGAGATTAGATTACCGAGAAACAGAACAAGGGTTTGGTTGGGGACTTTCGACACAGCTGAGGAGGCTGCAAGTGCTTATGACACTGCTGCCTATATGCTCCGAGGCGAATATGCTCATTTGAATTTTCCAGATTCAAAACATCAACTCAAGGCCAGTGCTTTGAATGGGAGTACAAGCACTACAGCTGCCCTGCTTGAAGCCAAGTTACAACTAGCAATATCACAACAGGCAAATAAGAAGACGAATATTATGGAGTCATCGTCTTCATCATCACCTCCATCACCAAAAAAGAAGAAGCACTCATCAGCTGATGATCGTCCTCAGAAGAACTCAAATTCCATCATCAACAGTAATTTGATCAGCCAAAACCAAATTACAAGAAAAGAGTGGCAGCTTGGATCTGATCAGGTGATATTGGAAAGCAAGAAAAGCCAGGATGTAATATCATCATCAGATATGGATGCTGTTCAGCTAAGTAGAATGCCATCTTTGGACATGGACATGATATGGGATGCTCTTCTCGTCTCTGATTAA

Protein Analysis

396

Amino Acids

43.52

Weight (kDa)

8.8

Isoelectric Point (pI)

54.14

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
AP2 PF00847 205 - 253 5e-14 AP2 domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Orthologous Genes (Group: OG0016683)

Species Orthologous Gene IDs
arabidopsis_thaliana AT4G13620
malus_domestica MD04G1009000.v1.1
prunus_persica Prupe.1G008400_v2.0.a1
pyrus_communis pycom04g00700
rosa_chinensis RchiOBHm_Chr4g0415231
rosa_laevigata RLG00000008088
rosa_multiflora Rmu_sc0001421.1_g000007
rosa_roxburghii Rroxscaffold_5G00359040
rosa_rugosa Rorug04G0132500
rosa_samantha Rh4AG193100 Rh4BG191500 Rh4CG204200 Rh4DG191000
rosa_wichuraiana Rw4G016420

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AclWI GGATC 4 cut(s) 67, 71, 338, 1060
AcoI YGGCCR 1 cut(s) 81
AcsI RAATTY 6 cut(s) 64, 205, 295, 304, 775, 988
AcuI CTGAAG 1 cut(s) 126
AfaI GTAC 3 cut(s) 55, 536, 826
AfiI CCNNNNNNNGG 1 cut(s) 485
AgsI TTSAA 6 cut(s) 215, 493, 775, 789, 817, 851
AjnI CCWGG 3 cut(s) 37, 430, 1086
AjuI GAANNNNNNNTTGG 2 cut(s) 670, 702
AloI GAACNNNNNNTCC 2 cut(s) 974, 1006
AluBI AGCT 8 cut(s) 239, 272, 610, 710, 839, 962, 1048, 1126
AluI AGCT 8 cut(s) 239, 272, 610, 710, 839, 962, 1048, 1126
Alw21I GWGCWC 1 cut(s) 423
Alw26I GTCTC 1 cut(s) 1183
AlwI GGATC 4 cut(s) 67, 71, 338, 1060
AoxI GGCC 2 cut(s) 81, 804
ApeKI GCWGC 4 cut(s) 719, 740, 839, 1045
ApoI RAATTY 6 cut(s) 64, 205, 295, 304, 775, 988
Asp700I GAANNNNTTC 1 cut(s) 219
AsuHPI GGTGA 6 cut(s) 86, 111, 404, 918, 927, 1075
BarI GAAGNNNNNNTAC 2 cut(s) 843, 875
BbsI GAAGAC 2 cut(s) 899, 909
Bbv12I GWGCWC 1 cut(s) 423
BbvCI CCTCAGC 1 cut(s) 711
BbvI GCAGC 4 cut(s) 706, 727, 826, 1057
BccI CCATC 8 cut(s) 298, 355, 487, 586, 601, 940, 1001, 1147
BciT130I CCWGG 3 cut(s) 39, 432, 1088
BclI TGATCA 4 cut(s) 250, 439, 1011, 1057
BcoDI GTCTC 1 cut(s) 1183
BfaI CTAG 3 cut(s) 129, 467, 866
BfmI CTRYAG 1 cut(s) 834
BisI GCNGC 4 cut(s) 720, 741, 840, 1046
BlsI GCNGC 4 cut(s) 721, 742, 841, 1047
Bme1390I CCNGG 3 cut(s) 39, 432, 1088
BmiI GGNNCC 1 cut(s) 327
BmrFI CCNGG 3 cut(s) 39, 432, 1088
BmsI GCATC 2 cut(s) 1105, 1156
BpiI GAAGAC 2 cut(s) 899, 909
BpmI CTGGAG 1 cut(s) 252
Bpu10I CCTNAGC 1 cut(s) 711
BpuEI CTTGAG 2 cut(s) 437, 785
BsaBI GATNNNNATC 1 cut(s) 106
BsaJI CCNNGG 1 cut(s) 754
BsaXI ACNNNNNCTCC 2 cut(s) 899, 929
Bsc4I CCNNNNNNNGG 1 cut(s) 485
Bse1I ACTGG 3 cut(s) 84, 506, 807
Bse8I GATNNNNATC 1 cut(s) 106
BseBI CCWGG 3 cut(s) 39, 432, 1088
BseDI CCNNGG 1 cut(s) 754
BseGI GGATG 4 cut(s) 12, 1096, 1120, 1171
BseJI GATNNNNATC 1 cut(s) 106
BseLI CCNNNNNNNGG 1 cut(s) 485
BseMII CTCAG 3 cut(s) 645, 702, 989
BseNI ACTGG 3 cut(s) 84, 506, 807
BseRI GAGGAG 3 cut(s) 373, 633, 728
BseXI GCAGC 4 cut(s) 706, 727, 826, 1057
BshFI GGCC 2 cut(s) 83, 806
BsiHKAI GWGCWC 1 cut(s) 423
BsiSI CCGG 1 cut(s) 80
BslFI GGGAC 1 cut(s) 709
BslI CCNNNNNNNGG 1 cut(s) 485
BsmAI GTCTC 1 cut(s) 1183
BsmBI CGTCTC 1 cut(s) 1183
BsmFI GGGAC 1 cut(s) 709
BsmI GAATGC 1 cut(s) 1140
BsnI GGCC 2 cut(s) 83, 806
Bsp1286I GDGCHC 1 cut(s) 423
BspANI GGCC 2 cut(s) 83, 806
BspCNI CTCAG 3 cut(s) 646, 703, 988
BspLI GGNNCC 1 cut(s) 327
BspPI GGATC 4 cut(s) 67, 71, 338, 1060
BspQI GCTCTTC 1 cut(s) 1176
BsrI ACTGG 3 cut(s) 84, 506, 807
BssECI CCNNGG 1 cut(s) 754
Bst2UI CCWGG 3 cut(s) 39, 432, 1088
Bst4CI ACNGT 4 cut(s) 117, 425, 539, 1004
Bst6I CTCTTC 1 cut(s) 1176
BstDEI CTNAG 5 cut(s) 506, 654, 711, 975, 1127
BstF5I GGATG 4 cut(s) 12, 1096, 1120, 1171
BstMAI GTCTC 1 cut(s) 1183
BstMWI GCNNNNNNNGC 3 cut(s) 716, 725, 1083
BstNI CCWGG 3 cut(s) 39, 432, 1088
BstSCI CCNGG 3 cut(s) 37, 430, 1086
BstSFI CTRYAG 1 cut(s) 834
BstV1I GCAGC 4 cut(s) 706, 727, 826, 1057
BstV2I GAAGAC 2 cut(s) 899, 909
BstX2I RGATCY 2 cut(s) 343, 1052
BstYI RGATCY 2 cut(s) 343, 1052
BsuRI GGCC 2 cut(s) 83, 806
BtsCI GGATG 4 cut(s) 12, 1096, 1120, 1171
BtsI GCAGTG 1 cut(s) 735
BtsIMutI CAGTG 5 cut(s) 113, 421, 544, 735, 814
Csp6I GTAC 3 cut(s) 54, 535, 825
CviAII CATG 4 cut(s) 30, 242, 1150, 1156
CviQI GTAC 3 cut(s) 54, 535, 825
DdeI CTNAG 5 cut(s) 506, 654, 711, 975, 1127
EaeI YGGCCR 1 cut(s) 81
Eam1104I CTCTTC 1 cut(s) 1176
EarI CTCTTC 1 cut(s) 1176
Eco57I CTGAAG 1 cut(s) 126
EcoRII CCWGG 3 cut(s) 37, 430, 1086
Esp3I CGTCTC 1 cut(s) 1183
FaeI CATG 4 cut(s) 33, 245, 1153, 1159
FaqI GGGAC 1 cut(s) 709
FatI CATG 4 cut(s) 29, 241, 1149, 1155
FbaI TGATCA 4 cut(s) 250, 439, 1011, 1057
Fnu4HI GCNGC 4 cut(s) 720, 741, 840, 1046
FokI GGATG 3 cut(s) 1103, 1127, 1178
Fsp4HI GCNGC 4 cut(s) 720, 741, 840, 1046
FspBI CTAG 3 cut(s) 129, 467, 866
GluI GCNGC 4 cut(s) 720, 741, 840, 1046
GsuI CTGGAG 1 cut(s) 252
HaeIII GGCC 2 cut(s) 83, 806
HapII CCGG 1 cut(s) 80
Hin1II CATG 4 cut(s) 33, 245, 1153, 1159
HincII GTYRAC 1 cut(s) 428
HindII GTYRAC 1 cut(s) 428
HinfI GANTC 5 cut(s) 125, 371, 559, 785, 908
HpaII CCGG 1 cut(s) 80
HphI GGTGA 6 cut(s) 86, 111, 404, 918, 927, 1075
Hpy166II GTNNAC 2 cut(s) 375, 428
Hpy188I TCNGA 7 cut(s) 250, 558, 755, 978, 1057, 1108, 1183
Hpy188III TCNNGA 2 cut(s) 549, 782
Hpy8I GTNNAC 2 cut(s) 375, 428
Hpy99I CGWCG 1 cut(s) 517
HpyAV CCTTC 2 cut(s) 19, 405
HpyCH4III ACNGT 4 cut(s) 117, 425, 539, 1004
HpyCH4IV ACGT 1 cut(s) 377
HpyCH4V TGCA 2 cut(s) 322, 722
HpyF10VI GCNNNNNNNGC 3 cut(s) 716, 725, 1083
HpyF3I CTNAG 5 cut(s) 506, 654, 711, 975, 1127
HpySE526I ACGT 1 cut(s) 377
Hsp92II CATG 4 cut(s) 33, 245, 1153, 1159
Ksp22I TGATCA 4 cut(s) 250, 439, 1011, 1057
LguI GCTCTTC 1 cut(s) 1176
LmnI GCTCC 1 cut(s) 756
Lsp1109I GCAGC 4 cut(s) 706, 727, 826, 1057
LweI GCATC 2 cut(s) 1105, 1156
MaeI CTAG 3 cut(s) 129, 467, 866
MaeII ACGT 1 cut(s) 377
MaeIII GTNAC 4 cut(s) 117, 161, 597, 858
MboII GAAGA 9 cut(s) 102, 129, 132, 555, 904, 909, 958, 991, 1163
MflI RGATCY 2 cut(s) 343, 1052
MhlI GDGCHC 1 cut(s) 423
MluCI AATT 8 cut(s) 64, 205, 295, 304, 775, 988, 1006, 1026
MlyI GAGTC 3 cut(s) 134, 380, 917
MroXI GAANNNNTTC 1 cut(s) 219
MseI TTAA 5 cut(s) 104, 231, 308, 389, 1186
MslI CAYNNNNRTG 1 cut(s) 1154
MspA1I CMGCKG 3 cut(s) 710, 839, 962
MspI CCGG 1 cut(s) 80
MspR9I CCNGG 3 cut(s) 39, 432, 1088
Mva1269I GAATGC 1 cut(s) 1140
MvaI CCWGG 3 cut(s) 39, 432, 1088
MwoI GCNNNNNNNGC 3 cut(s) 716, 725, 1083
NlaIII CATG 4 cut(s) 33, 245, 1153, 1159
NlaIV GGNNCC 1 cut(s) 327
NmuCI GTSAC 2 cut(s) 117, 597
PciSI GCTCTTC 1 cut(s) 1176
PctI GAATGC 1 cut(s) 1140
PdmI GAANNNNTTC 1 cut(s) 219
PfeI GAWTC 2 cut(s) 559, 785
PfoI TCCNGGA 1 cut(s) 37
PkrI GCNGC 4 cut(s) 721, 742, 841, 1047
PleI GAGTC 3 cut(s) 133, 379, 916
PpsI GAGTC 3 cut(s) 133, 379, 916
Psp6I CCWGG 3 cut(s) 37, 430, 1086
PspGI CCWGG 3 cut(s) 37, 430, 1086
PspN4I GGNNCC 1 cut(s) 327
PsuI RGATCY 2 cut(s) 343, 1052
PvuII CAGCTG 3 cut(s) 710, 839, 962
RsaI GTAC 3 cut(s) 55, 536, 826
RsaNI GTAC 3 cut(s) 54, 535, 825
RseI CAYNNNNRTG 1 cut(s) 1154
SapI GCTCTTC 1 cut(s) 1176
SaqAI TTAA 5 cut(s) 104, 231, 308, 389, 1186
SatI GCNGC 4 cut(s) 720, 741, 840, 1046
SchI GAGTC 3 cut(s) 134, 380, 917
ScrFI CCNGG 3 cut(s) 39, 432, 1088
SduI GDGCHC 1 cut(s) 423
SfaNI GCATC 2 cut(s) 1105, 1156
SfcI CTRYAG 1 cut(s) 834
SmiMI CAYNNNNRTG 1 cut(s) 1154
SmlI CTYRAG 2 cut(s) 416, 800
SmoI CTYRAG 2 cut(s) 416, 800
Sse9I AATT 8 cut(s) 64, 205, 295, 304, 775, 988, 1006, 1026
SspI AATATT 1 cut(s) 901
SspMI CTAG 3 cut(s) 129, 467, 866
StyD4I CCNGG 3 cut(s) 37, 430, 1086
TaaI ACNGT 4 cut(s) 117, 425, 539, 1004
TaiI ACGT 1 cut(s) 380
TaqI TCGA 2 cut(s) 62, 702
TasI AATT 8 cut(s) 64, 205, 295, 304, 775, 988, 1006, 1026
TatI WGTACW 2 cut(s) 534, 824
TfiI GAWTC 2 cut(s) 559, 785
Tru1I TTAA 5 cut(s) 104, 231, 308, 389, 1186
Tru9I TTAA 5 cut(s) 104, 231, 308, 389, 1186
TscAI CASTG 5 cut(s) 120, 428, 544, 742, 814
TseFI GTSAC 2 cut(s) 117, 597
TseI GCWGC 4 cut(s) 719, 740, 839, 1045
Tsp45I GTSAC 2 cut(s) 117, 597
TspDTI ATGAA 3 cut(s) 387, 545, 909
TspRI CASTG 5 cut(s) 120, 428, 544, 742, 814
XapI RAATTY 6 cut(s) 64, 205, 295, 304, 775, 988
XmnI GAANNNNTTC 1 cut(s) 219
XspI CTAG 3 cut(s) 129, 467, 866
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.