pycom04g00700

ethylene-responsive transcription factor

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr4
OG0016683
Physical Location & Seq
Reverse (-)
640052 .. 641347
1296 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom04g00700.1

Sequence Viewer

Length: 1296 bp
ATGGAGGAAAAGTTTCCCAAGATGGAAACCTTCATAGAAAAGCAATTGCCATACTCTTATTTTCCAAGAATGGCAACTAGTAACAATATTGGATCAAATTTGTTTGGTGATCATCCAGCCAGCTGGGGTGGCAGTGGATCAAAATCGCCACTGTCATCGGAGTCTAGTAGCTCTTCCGCAGAGGATGTTTCTCTTTCGGGTTCCAATTTGATCAACAACACAAGCAGTCCTTTTGTTCCTCTAAATTTCTTGGAAACATTTCCACAGCTGACTAGAGCTCAAGTTTCTGATCAACCACCTACTTCTCCACCTTCCTTGTCATCAAAACCATCGAGATTTCCGAAATTAACTTTGTTTTTGCAGCAACCAAGTCTGTTAGATCCATCATCATCATCATCCCCATCGTCATCGTCGTCCATGCAAGTTGAAGTTGTAAACTCACTTGGCAAGACCAAAAGGTGTAATGAGTTATCCTCTTTATTAACCCCTTCATTTCCTGCTCCTCAAGCAATTGATCAAATTCAGCATCAGCCGGGCATGATTGAGTGGCTTAAAATTAATCAAACTTTAGCAAACCACTCATCCAAAGGCTTCAATGATTACTGGCTAAGCACGACGAAGACGCAGCCAATGAAGCACACTGGAAGATCATCATCAAGATTGCATCAGCAGCAGATCAATCAACAACAAAAGCAGCCTAATAACAATTTGTCCTCCTCCTCCTCATCATCACAAGGAAAGCTATTTAGAGGAGTTAGGCAGAGGCATTGGGGGAAATGGGTGGCTGAGATTCGATTACCAAGAAACCGAACAAGGGTTTGGTTGGGGACCTTTGACACCGCTGAGGAGGCTGCCATTGCATATGACACCGCAGCTTATATATTGCGCGGCGAACATGCACAGTTGAATCTCCCAGATTTGAAGCATCAACTTAAGGCCAGTGCTTTGAAAGGAACCACAGCTGCTCTTCTTGAAGCAAAGCTGCAAGCAATATCATCATCACAAGCACATAAGAAGGCCATCAAGGAGTCCTCTTCGTCCGAATCAGCGTCATCAAACAAGCACTCAGCGGGTGATGGCGGCGATCACAATAATCTCATGATCAATAGTAATTTGAGCCAAAACGCTACAAGAAAAGAGTGGCAGTTTGAGTTGGAGAGCAAGGTTGGATCTCATCACCATCACCACCAGCAGGATTTAGTATCAGCAGCAGATGTGGATGCTGTTCAATTAAGTAGAATGCCCTCCTTGGACATGGACATGATATGGGATGCTCTTCTGGTCTCTGATTCCTAA

Protein Analysis

432

Amino Acids

47.38

Weight (kDa)

8.77

Isoelectric Point (pI)

58.07

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
AP2 PF00847 249 - 297 4.7e-13 AP2 domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0016683)

Species Orthologous Gene IDs
arabidopsis_thaliana AT4G13620
malus_domestica MD04G1009000.v1.1
prunus_persica Prupe.1G008400_v2.0.a1
pyrus_communis pycom04g00700
rosa_chinensis RchiOBHm_Chr4g0415231
rosa_laevigata RLG00000008088
rosa_multiflora Rmu_sc0001421.1_g000007
rosa_roxburghii Rroxscaffold_5G00359040
rosa_rugosa Rorug04G0132500
rosa_samantha Rh4AG193100 Rh4BG191500 Rh4CG204200 Rh4DG191000
rosa_wichuraiana Rw4G016420

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccII CGCG 1 cut(s) 888
AciI CCGC 6 cut(s) 177, 840, 870, 888, 1070, 1080
AclWI GGATC 4 cut(s) 100, 145, 374, 1177
AcsI RAATTY 3 cut(s) 97, 244, 519
AfiI CCNNNNNNNGG 2 cut(s) 814, 1192
AflII CTTAAG 1 cut(s) 932
AgsI TTSAA 7 cut(s) 428, 595, 907, 922, 949, 974, 1229
AhlI ACTAGT 1 cut(s) 77
AjuI GAANNNNNNNTTGG 2 cut(s) 802, 834
AluBI AGCT 8 cut(s) 123, 171, 268, 278, 742, 875, 962, 982
AluI AGCT 8 cut(s) 123, 171, 268, 278, 742, 875, 962, 982
Alw21I GWGCWC 1 cut(s) 280
Alw26I GTCTC 1 cut(s) 1288
AlwI GGATC 4 cut(s) 100, 145, 374, 1177
AoxI GGCC 2 cut(s) 936, 1017
ApeKI GCWGC 9 cut(s) 361, 625, 670, 694, 851, 872, 962, 982, 1208
ApoI RAATTY 3 cut(s) 97, 244, 519
AseI ATTAAT 1 cut(s) 558
Asp700I GAANNNNTTC 2 cut(s) 12, 258
AspLEI GCGC 1 cut(s) 888
AspS9I GGNCC 1 cut(s) 828
AsuC2I CCSGG 1 cut(s) 534
AsuHPI GGTGA 4 cut(s) 119, 1085, 1169, 1175
AvaII GGWCC 1 cut(s) 828
BanII GRGCYC 1 cut(s) 280
BbsI GAAGAC 1 cut(s) 626
Bbv12I GWGCWC 1 cut(s) 280
BbvCI CCTCAGC 1 cut(s) 843
BbvI GCAGC 9 cut(s) 373, 637, 682, 706, 838, 884, 949, 969, 1220
BccI CCATC 7 cut(s) 16, 337, 391, 409, 1028, 1070, 1188
BclI TGATCA 5 cut(s) 109, 210, 289, 514, 1101
BcnI CCSGG 1 cut(s) 534
BcoDI GTCTC 1 cut(s) 1288
BcuI ACTAGT 1 cut(s) 77
BfaI CTAG 3 cut(s) 78, 165, 273
BfrI CTTAAG 1 cut(s) 932
BlpI GCTNAGC 1 cut(s) 608
Bme1390I CCNGG 1 cut(s) 534
Bme18I GGWCC 1 cut(s) 828
BmgT120I GGNCC 1 cut(s) 828
BmiI GGNNCC 3 cut(s) 202, 829, 955
BmrFI CCNGG 1 cut(s) 534
BmsI GCATC 5 cut(s) 535, 673, 934, 1210, 1261
BpiI GAAGAC 1 cut(s) 626
BplI GAGNNNNNCTC 2 cut(s) 458, 490
Bpu10I CCTNAGC 1 cut(s) 843
Bpu1102I GCTNAGC 1 cut(s) 608
BpuEI CTTGAG 2 cut(s) 264, 489
BpuMI CCSGG 1 cut(s) 534
BsaBI GATNNNNATC 2 cut(s) 142, 652
BsaI GGTCTC 1 cut(s) 1288
BsaJI CCNNGG 1 cut(s) 1248
BsaXI ACNNNNNCTCC 1 cut(s) 26
Bsc4I CCNNNNNNNGG 2 cut(s) 814, 1192
Bse1I ACTGG 3 cut(s) 608, 646, 939
Bse3DI GCAATG 1 cut(s) 855
Bse8I GATNNNNATC 2 cut(s) 142, 652
BseDI CCNNGG 1 cut(s) 1248
BseGI GGATG 6 cut(s) 112, 190, 395, 581, 1225, 1276
BseJI GATNNNNATC 2 cut(s) 142, 652
BseLI CCNNNNNNNGG 2 cut(s) 814, 1192
BseMI GCAATG 1 cut(s) 855
BseMII CTCAG 3 cut(s) 777, 834, 1080
BseNI ACTGG 3 cut(s) 608, 646, 939
BseRI GAGGAG 6 cut(s) 492, 706, 709, 712, 765, 860
BseXI GCAGC 9 cut(s) 373, 637, 682, 706, 838, 884, 949, 969, 1220
BseYI CCCAGC 1 cut(s) 123
Bsh1236I CGCG 1 cut(s) 888
BshFI GGCC 2 cut(s) 938, 1019
BsiHKAI GWGCWC 1 cut(s) 280
BsiSI CCGG 1 cut(s) 533
BslFI GGGAC 1 cut(s) 841
BslI CCNNNNNNNGG 2 cut(s) 814, 1192
BsmAI GTCTC 1 cut(s) 1288
BsmFI GGGAC 1 cut(s) 841
BsmI GAATGC 1 cut(s) 1245
BsnI GGCC 2 cut(s) 938, 1019
Bso31I GGTCTC 1 cut(s) 1288
Bsp1286I GDGCHC 1 cut(s) 280
Bsp1720I GCTNAGC 1 cut(s) 608
BspACI CCGC 6 cut(s) 177, 840, 870, 888, 1070, 1080
BspANI GGCC 2 cut(s) 938, 1019
BspCNI CTCAG 3 cut(s) 778, 835, 1079
BspFNI CGCG 1 cut(s) 888
BspHI TCATGA 1 cut(s) 1098
BspLI GGNNCC 3 cut(s) 202, 829, 955
BspPI GGATC 4 cut(s) 100, 145, 374, 1177
BspQI GCTCTTC 3 cut(s) 178, 972, 1281
BspTI CTTAAG 1 cut(s) 932
BspTNI GGTCTC 1 cut(s) 1288
BsrDI GCAATG 1 cut(s) 855
BsrI ACTGG 3 cut(s) 608, 646, 939
BssECI CCNNGG 1 cut(s) 1248
BssT1I CCWWGG 1 cut(s) 1248
Bst4CI ACNGT 2 cut(s) 153, 903
Bst6I CTCTTC 4 cut(s) 178, 972, 1039, 1281
BstAFI CTTAAG 1 cut(s) 932
BstC8I GCNNGC 2 cut(s) 121, 987
BstDEI CTNAG 4 cut(s) 608, 786, 843, 1066
BstF5I GGATG 6 cut(s) 112, 190, 395, 581, 1225, 1276
BstFNI CGCG 1 cut(s) 888
BstHHI GCGC 1 cut(s) 888
BstMAI GTCTC 1 cut(s) 1288
BstMWI GCNNNNNNNGC 6 cut(s) 129, 506, 634, 670, 848, 857
BstNSI RCATGY 1 cut(s) 899
BstSCI CCNGG 1 cut(s) 532
BstUI CGCG 1 cut(s) 888
BstV1I GCAGC 9 cut(s) 373, 637, 682, 706, 838, 884, 949, 969, 1220
BstV2I GAAGAC 1 cut(s) 626
BstX2I RGATCY 2 cut(s) 379, 1169
BstXI CCANNNNNNTGG 1 cut(s) 123
BstYI RGATCY 2 cut(s) 379, 1169
BsuRI GGCC 2 cut(s) 938, 1019
BtsCI GGATG 6 cut(s) 112, 190, 395, 581, 1225, 1276
BtsI GCAGTG 1 cut(s) 139
BtsIMutI CAGTG 4 cut(s) 139, 149, 639, 946
Cac8I GCNNGC 2 cut(s) 121, 987
CciI TCATGA 1 cut(s) 1098
CfoI GCGC 1 cut(s) 888
Cfr13I GGNCC 1 cut(s) 828
CseI GACGC 2 cut(s) 631, 1038
CspCI CAANNNNNGTGG 2 cut(s) 297, 332
CviAII CATG 6 cut(s) 418, 538, 896, 1099, 1255, 1261
DdeI CTNAG 4 cut(s) 608, 786, 843, 1066
Eam1104I CTCTTC 4 cut(s) 178, 972, 1039, 1281
EarI CTCTTC 4 cut(s) 178, 972, 1039, 1281
Ecl136II GAGCTC 1 cut(s) 278
Eco130I CCWWGG 1 cut(s) 1248
Eco24I GRGCYC 1 cut(s) 280
Eco31I GGTCTC 1 cut(s) 1288
Eco47I GGWCC 1 cut(s) 828
Eco53kI GAGCTC 1 cut(s) 278
EcoICRI GAGCTC 1 cut(s) 278
EcoO109I RGGNCCY 1 cut(s) 828
EcoT14I CCWWGG 1 cut(s) 1248
EcoT38I GRGCYC 1 cut(s) 280
ErhI CCWWGG 1 cut(s) 1248
FaeI CATG 6 cut(s) 421, 541, 899, 1102, 1258, 1264
FalI AAGNNNNNCTT 2 cut(s) 214, 246
FaqI GGGAC 1 cut(s) 841
FatI CATG 6 cut(s) 417, 537, 895, 1098, 1254, 1260
FauI CCCGC 1 cut(s) 1063
FauNDI CATATG 1 cut(s) 862
FbaI TGATCA 5 cut(s) 109, 210, 289, 514, 1101
FokI GGATG 6 cut(s) 99, 197, 382, 568, 1232, 1283
FriOI GRGCYC 1 cut(s) 280
FspBI CTAG 3 cut(s) 78, 165, 273
GlaI GCGC 1 cut(s) 887
GsaI CCCAGC 1 cut(s) 127
HaeIII GGCC 2 cut(s) 938, 1019
HapII CCGG 1 cut(s) 533
HgaI GACGC 2 cut(s) 631, 1038
HhaI GCGC 1 cut(s) 888
Hin1II CATG 6 cut(s) 421, 541, 899, 1102, 1258, 1264
Hin6I GCGC 1 cut(s) 886
HinP1I GCGC 1 cut(s) 886
HinfI GANTC 6 cut(s) 161, 790, 907, 1028, 1043, 1289
HpaII CCGG 1 cut(s) 533
HphI GGTGA 4 cut(s) 119, 1085, 1169, 1175
Hpy166II GTNNAC 1 cut(s) 436
Hpy188I TCNGA 5 cut(s) 160, 289, 342, 1042, 1288
Hpy188III TCNNGA 4 cut(s) 333, 657, 971, 1099
Hpy8I GTNNAC 1 cut(s) 436
Hpy99I CGWCG 2 cut(s) 415, 619
HpyAV CCTTC 4 cut(s) 40, 321, 498, 1009
HpyCH4III ACNGT 2 cut(s) 153, 903
HpyCH4V TGCA 6 cut(s) 361, 421, 664, 860, 899, 985
HpyF10VI GCNNNNNNNGC 6 cut(s) 129, 506, 634, 670, 848, 857
HpyF3I CTNAG 4 cut(s) 608, 786, 843, 1066
Hsp92II CATG 6 cut(s) 421, 541, 899, 1102, 1258, 1264
HspAI GCGC 1 cut(s) 886
Ksp22I TGATCA 5 cut(s) 109, 210, 289, 514, 1101
LguI GCTCTTC 3 cut(s) 178, 972, 1281
LmnI GCTCC 1 cut(s) 505
Lsp1109I GCAGC 9 cut(s) 373, 637, 682, 706, 838, 884, 949, 969, 1220
LweI GCATC 5 cut(s) 535, 673, 934, 1210, 1261
MaeI CTAG 3 cut(s) 78, 165, 273
MaeIII GTNAC 1 cut(s) 80
MboII GAAGA 6 cut(s) 165, 631, 657, 959, 1026, 1268
MfeI CAATTG 2 cut(s) 44, 510
MflI RGATCY 2 cut(s) 379, 1169
MhlI GDGCHC 1 cut(s) 280
MlyI GAGTC 2 cut(s) 170, 1037
MmeI TCCRAC 2 cut(s) 1134, 1147
MroXI GAANNNNTTC 2 cut(s) 12, 258
MseI TTAA 6 cut(s) 347, 482, 552, 558, 933, 1232
MslI CAYNNNNRTG 1 cut(s) 1259
MspA1I CMGCKG 5 cut(s) 123, 268, 842, 962, 1070
MspCI CTTAAG 1 cut(s) 932
MspI CCGG 1 cut(s) 533
MspR9I CCNGG 1 cut(s) 534
MunI CAATTG 2 cut(s) 44, 510
Mva1269I GAATGC 1 cut(s) 1245
MvnI CGCG 1 cut(s) 888
MwoI GCNNNNNNNGC 6 cut(s) 129, 506, 634, 670, 848, 857
NciI CCSGG 1 cut(s) 534
NdeI CATATG 1 cut(s) 862
NlaIII CATG 6 cut(s) 421, 541, 899, 1102, 1258, 1264
NlaIV GGNNCC 3 cut(s) 202, 829, 955
NspI RCATGY 1 cut(s) 899
PagI TCATGA 1 cut(s) 1098
PciSI GCTCTTC 3 cut(s) 178, 972, 1281
PcsI WCGNNNNNNNCGW 2 cut(s) 338, 410
PctI GAATGC 1 cut(s) 1245
PdmI GAANNNNTTC 2 cut(s) 12, 258
PfeI GAWTC 4 cut(s) 790, 907, 1043, 1289
PleI GAGTC 2 cut(s) 169, 1036
PpsI GAGTC 2 cut(s) 169, 1036
PpuMI RGGWCCY 1 cut(s) 828
PshBI ATTAAT 1 cut(s) 558
Psp124BI GAGCTC 1 cut(s) 280
Psp5II RGGWCCY 1 cut(s) 828
PspFI CCCAGC 1 cut(s) 123
PspN4I GGNNCC 3 cut(s) 202, 829, 955
PspPI GGNCC 1 cut(s) 828
PspPPI RGGWCCY 1 cut(s) 828
PsuI RGATCY 2 cut(s) 379, 1169
PvuII CAGCTG 3 cut(s) 123, 268, 962
RseI CAYNNNNRTG 1 cut(s) 1259
SacI GAGCTC 1 cut(s) 280
SapI GCTCTTC 3 cut(s) 178, 972, 1281
SaqAI TTAA 6 cut(s) 347, 482, 552, 558, 933, 1232
Sau96I GGNCC 1 cut(s) 828
SchI GAGTC 2 cut(s) 170, 1037
ScrFI CCNGG 1 cut(s) 534
SduI GDGCHC 1 cut(s) 280
SfaNI GCATC 5 cut(s) 535, 673, 934, 1210, 1261
SinI GGWCC 1 cut(s) 828
SmiMI CAYNNNNRTG 1 cut(s) 1259
SmlI CTYRAG 3 cut(s) 279, 504, 932
SmoI CTYRAG 3 cut(s) 279, 504, 932
SpeI ACTAGT 1 cut(s) 77
SsiI CCGC 6 cut(s) 177, 840, 870, 888, 1070, 1080
SspI AATATT 1 cut(s) 88
SspMI CTAG 3 cut(s) 78, 165, 273
SstI GAGCTC 1 cut(s) 280
StyD4I CCNGG 1 cut(s) 532
StyI CCWWGG 1 cut(s) 1248
TaaI ACNGT 2 cut(s) 153, 903
TaqI TCGA 2 cut(s) 332, 793
TauI GCSGC 2 cut(s) 891, 1083
TfiI GAWTC 4 cut(s) 790, 907, 1043, 1289
Tru1I TTAA 6 cut(s) 347, 482, 552, 558, 933, 1232
Tru9I TTAA 6 cut(s) 347, 482, 552, 558, 933, 1232
TscAI CASTG 4 cut(s) 139, 156, 646, 946
TseI GCWGC 9 cut(s) 361, 625, 670, 694, 851, 872, 962, 982, 1208
TspDTI ATGAA 3 cut(s) 22, 480, 647
TspRI CASTG 4 cut(s) 139, 156, 646, 946
Vha464I CTTAAG 1 cut(s) 932
VpaK11BI GGWCC 1 cut(s) 828
VspI ATTAAT 1 cut(s) 558
XapI RAATTY 3 cut(s) 97, 244, 519
XceI RCATGY 1 cut(s) 899
XmnI GAANNNNTTC 2 cut(s) 12, 258
XspI CTAG 3 cut(s) 78, 165, 273
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.