Rw4G016420

ethylene-responsive transcription factor

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr4
OG0016683
Physical Location & Seq
Forward (+)
37499256 .. 37500602
1347 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw4G016420.1

Sequence Viewer

Length: 1209 bp
ATGGATGATAAGTTTCCCAAGATGGAAACCTTCATCCCAAAGCAGTTGCCATATTTTCCAGGAATGGCAAGTAGTACCGGATCGAATTTTCTCGGTGATCCGGCCGGTTGGGAAGTCGGTGGATTAAAATCATCACTGTCACCAGAGTCTAGCACTTCTTCTTCAGCAGAGGAAGTTTCTGGTAACTCCACTCTGATAAACAAAACAAATCCAGTTGTCCCTGTGAATTTCCTTGAAACATTTCCTGTGCTTAACAAAGCTCATGTTTCTGATCAACCACCTACTTCTCCAGCTTCCTTGTCATCAAAGCCATCAAATTTCCCAAATTTAACTCTACTTTTGCAGGAACCAACTACCCTTTTAGATCCATCATCAACCAAAAAGTGTGATGAGTCCATGTTCTCCTCTTTAAGCCCCTCATTTCCTATACCTCACCTTGAGCACTGTCAACCAGGCTTTGATCAATGGCTCAAAATCAGTCAAACTCTAGCAAAAAATCCATCTAAAGGTTTCAATGATTACTGGCTAAGTGCGACGAAAACACAGCCAATGAAGTACAGTGGAAGATCAAGATTTCAGAATCAACAACAGAAACAGCCATCAGCACCAATCCCATCGTCACAAGGAAAGCTATTTAGAGGAGTGAGGCAAAGGCATTGGGGAAAATGGGTGGCTGAGATTAGATTACCGAGAAACAGAACAAGGGTTTGGTTGGGGACTTTCGACACAGCTGAGGAGGCTGCAAGTGCTTATGACACTGCTGCCTATATGCTCCGAGGCGAATATGCTCATTTGAACTTTCCAGATTCAAAACATCAACTCAAGGCCAATGCTTTGAATGGGAATACAAGCACTACAGCTGCCCTGCTTGAAGCCAAGTTACAACTAGCAATATCACAACAGGCAAATAAGAAGACGAATATTATGGAGTCATCGTCTTCATCATCACCTCCATCACCAAAAAAGAAGAAGCACTCATCAGCCGATGATCGTCATCAGAAGAACTCAAATTCCATCATCAATAGTAATTTGATCAGCCAAAACCAAATTACAAGAAAAGAGTGGCAGCTTGGATCTGATCAGGTGATATTTGAAAGCAAGAAAAGCCAGGATGTAATATCATCATCAGATATGGATGCTGTTCAGCTAAGTAGAATGCCATCTTTGGACATGGACATGATATGGGATGCTCTTCTGGTCTCTGATTAA

Protein Analysis

402

Amino Acids

44.44

Weight (kDa)

8.97

Isoelectric Point (pI)

54.06

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
AP2 PF00847 212 - 260 5.2e-14 AP2 domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0016683)

Species Orthologous Gene IDs
arabidopsis_thaliana AT4G13620
malus_domestica MD04G1009000.v1.1
prunus_persica Prupe.1G008400_v2.0.a1
pyrus_communis pycom04g00700
rosa_chinensis RchiOBHm_Chr4g0415231
rosa_laevigata RLG00000008088
rosa_multiflora Rmu_sc0001421.1_g000007
rosa_roxburghii Rroxscaffold_5G00359040
rosa_rugosa Rorug04G0132500
rosa_samantha Rh4AG193100 Rh4BG191500 Rh4CG204200 Rh4DG191000
rosa_wichuraiana Rw4G016420

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AclWI GGATC 4 cut(s) 88, 92, 359, 1081
AcoI YGGCCR 1 cut(s) 102
AcsI RAATTY 5 cut(s) 85, 226, 316, 325, 1009
AcuI CTGAAG 1 cut(s) 147
AfaI GTAC 2 cut(s) 76, 557
AfiI CCNNNNNNNGG 1 cut(s) 506
AgsI TTSAA 7 cut(s) 236, 514, 796, 810, 838, 872, 1094
AjnI CCWGG 3 cut(s) 58, 451, 1107
AjuI GAANNNNNNNTTGG 2 cut(s) 691, 723
AloI GAACNNNNNNTCC 2 cut(s) 995, 1027
AluBI AGCT 7 cut(s) 260, 293, 631, 731, 860, 1069, 1147
AluI AGCT 7 cut(s) 260, 293, 631, 731, 860, 1069, 1147
Alw21I GWGCWC 1 cut(s) 444
Alw26I GTCTC 1 cut(s) 1204
AlwI GGATC 4 cut(s) 88, 92, 359, 1081
AoxI GGCC 2 cut(s) 102, 825
ApeKI GCWGC 4 cut(s) 740, 761, 860, 1066
ApoI RAATTY 5 cut(s) 85, 226, 316, 325, 1009
Asp700I GAANNNNTTC 1 cut(s) 240
AsuHPI GGTGA 6 cut(s) 107, 132, 425, 939, 948, 1096
BarI GAAGNNNNNNTAC 2 cut(s) 864, 896
BbsI GAAGAC 2 cut(s) 920, 930
Bbv12I GWGCWC 1 cut(s) 444
BbvCI CCTCAGC 1 cut(s) 732
BbvI GCAGC 4 cut(s) 727, 748, 847, 1078
BccI CCATC 9 cut(s) 16, 319, 376, 508, 607, 622, 961, 1022, 1168
BciT130I CCWGG 3 cut(s) 60, 453, 1109
BclI TGATCA 4 cut(s) 271, 460, 1032, 1078
BcoDI GTCTC 1 cut(s) 1204
BfaI CTAG 3 cut(s) 150, 488, 887
BfmI CTRYAG 1 cut(s) 855
BisI GCNGC 4 cut(s) 741, 762, 861, 1067
BlsI GCNGC 4 cut(s) 742, 763, 862, 1068
Bme1390I CCNGG 3 cut(s) 60, 453, 1109
BmiI GGNNCC 1 cut(s) 348
BmrFI CCNGG 3 cut(s) 60, 453, 1109
BmsI GCATC 2 cut(s) 1126, 1177
BpiI GAAGAC 2 cut(s) 920, 930
BpmI CTGGAG 1 cut(s) 273
Bpu10I CCTNAGC 1 cut(s) 732
BpuEI CTTGAG 2 cut(s) 458, 806
BsaBI GATNNNNATC 2 cut(s) 127, 993
BsaI GGTCTC 1 cut(s) 1204
BsaJI CCNNGG 1 cut(s) 775
BsaWI WCCGGW 1 cut(s) 77
BsaXI ACNNNNNCTCC 2 cut(s) 920, 950
Bsc4I CCNNNNNNNGG 1 cut(s) 506
Bse118I RCCGGY 1 cut(s) 104
Bse1I ACTGG 2 cut(s) 212, 527
Bse8I GATNNNNATC 2 cut(s) 127, 993
BseBI CCWGG 3 cut(s) 60, 453, 1109
BseDI CCNNGG 1 cut(s) 775
BseGI GGATG 5 cut(s) 10, 33, 1117, 1141, 1192
BseJI GATNNNNATC 2 cut(s) 127, 993
BseLI CCNNNNNNNGG 1 cut(s) 506
BseMII CTCAG 2 cut(s) 666, 723
BseNI ACTGG 2 cut(s) 212, 527
BseRI GAGGAG 3 cut(s) 394, 654, 749
BseX3I CGGCCG 1 cut(s) 102
BseXI GCAGC 4 cut(s) 727, 748, 847, 1078
Bsh1285I CGRYCG 1 cut(s) 105
BshFI GGCC 2 cut(s) 104, 827
BsiEI CGRYCG 1 cut(s) 105
BsiHKAI GWGCWC 1 cut(s) 444
BsiSI CCGG 3 cut(s) 78, 101, 105
BslFI GGGAC 2 cut(s) 203, 730
BslI CCNNNNNNNGG 1 cut(s) 506
BsmAI GTCTC 1 cut(s) 1204
BsmFI GGGAC 2 cut(s) 203, 730
BsmI GAATGC 1 cut(s) 1161
BsnI GGCC 2 cut(s) 104, 827
Bso31I GGTCTC 1 cut(s) 1204
Bsp1286I GDGCHC 1 cut(s) 444
BspANI GGCC 2 cut(s) 104, 827
BspCNI CTCAG 2 cut(s) 667, 724
BspLI GGNNCC 1 cut(s) 348
BspPI GGATC 4 cut(s) 88, 92, 359, 1081
BspQI GCTCTTC 1 cut(s) 1197
BspTNI GGTCTC 1 cut(s) 1204
BsrFI RCCGGY 1 cut(s) 104
BsrI ACTGG 2 cut(s) 212, 527
BssAI RCCGGY 1 cut(s) 104
BssECI CCNNGG 1 cut(s) 775
Bst2UI CCWGG 3 cut(s) 60, 453, 1109
Bst4CI ACNGT 3 cut(s) 138, 446, 560
Bst6I CTCTTC 1 cut(s) 1197
BstDEI CTNAG 4 cut(s) 527, 675, 732, 1148
BstF5I GGATG 5 cut(s) 10, 33, 1117, 1141, 1192
BstMAI GTCTC 1 cut(s) 1204
BstMCI CGRYCG 1 cut(s) 105
BstMWI GCNNNNNNNGC 3 cut(s) 737, 746, 1104
BstNI CCWGG 3 cut(s) 60, 453, 1109
BstSCI CCNGG 3 cut(s) 58, 451, 1107
BstSFI CTRYAG 1 cut(s) 855
BstV1I GCAGC 4 cut(s) 727, 748, 847, 1078
BstV2I GAAGAC 2 cut(s) 920, 930
BstX2I RGATCY 2 cut(s) 364, 1073
BstYI RGATCY 2 cut(s) 364, 1073
BstZI CGGCCG 1 cut(s) 102
BsuRI GGCC 2 cut(s) 104, 827
BtsCI GGATG 5 cut(s) 10, 33, 1117, 1141, 1192
BtsI GCAGTG 1 cut(s) 756
BtsIMutI CAGTG 4 cut(s) 134, 442, 565, 756
Cfr10I RCCGGY 1 cut(s) 104
Csp6I GTAC 2 cut(s) 75, 556
CviAII CATG 4 cut(s) 263, 397, 1171, 1177
CviQI GTAC 2 cut(s) 75, 556
DdeI CTNAG 4 cut(s) 527, 675, 732, 1148
EaeI YGGCCR 1 cut(s) 102
EagI CGGCCG 1 cut(s) 102
Eam1104I CTCTTC 1 cut(s) 1197
EarI CTCTTC 1 cut(s) 1197
EclXI CGGCCG 1 cut(s) 102
Eco31I GGTCTC 1 cut(s) 1204
Eco52I CGGCCG 1 cut(s) 102
Eco57I CTGAAG 1 cut(s) 147
EcoRII CCWGG 3 cut(s) 58, 451, 1107
FaeI CATG 4 cut(s) 266, 400, 1174, 1180
FaqI GGGAC 2 cut(s) 203, 730
FatI CATG 4 cut(s) 262, 396, 1170, 1176
FbaI TGATCA 4 cut(s) 271, 460, 1032, 1078
Fnu4HI GCNGC 4 cut(s) 741, 762, 861, 1067
FokI GGATG 5 cut(s) 17, 20, 1124, 1148, 1199
Fsp4HI GCNGC 4 cut(s) 741, 762, 861, 1067
FspBI CTAG 3 cut(s) 150, 488, 887
GluI GCNGC 4 cut(s) 741, 762, 861, 1067
GsuI CTGGAG 1 cut(s) 273
HaeIII GGCC 2 cut(s) 104, 827
HapII CCGG 3 cut(s) 78, 101, 105
Hin1II CATG 4 cut(s) 266, 400, 1174, 1180
HincII GTYRAC 1 cut(s) 449
HindII GTYRAC 1 cut(s) 449
HinfI GANTC 5 cut(s) 146, 392, 580, 806, 929
HpaII CCGG 3 cut(s) 78, 101, 105
HphI GGTGA 6 cut(s) 107, 132, 425, 939, 948, 1096
Hpy166II GTNNAC 1 cut(s) 449
Hpy188I TCNGA 8 cut(s) 195, 271, 579, 776, 999, 1078, 1129, 1204
Hpy188III TCNNGA 2 cut(s) 570, 803
Hpy8I GTNNAC 1 cut(s) 449
Hpy99I CGWCG 1 cut(s) 538
HpyAV CCTTC 1 cut(s) 40
HpyCH4III ACNGT 3 cut(s) 138, 446, 560
HpyCH4V TGCA 2 cut(s) 343, 743
HpyF10VI GCNNNNNNNGC 3 cut(s) 737, 746, 1104
HpyF3I CTNAG 4 cut(s) 527, 675, 732, 1148
Hsp92II CATG 4 cut(s) 266, 400, 1174, 1180
Ksp22I TGATCA 4 cut(s) 271, 460, 1032, 1078
LguI GCTCTTC 1 cut(s) 1197
LmnI GCTCC 1 cut(s) 777
Lsp1109I GCAGC 4 cut(s) 727, 748, 847, 1078
LweI GCATC 2 cut(s) 1126, 1177
MaeI CTAG 3 cut(s) 150, 488, 887
MaeIII GTNAC 4 cut(s) 138, 182, 618, 879
MboII GAAGA 8 cut(s) 150, 153, 576, 925, 930, 979, 1012, 1184
MflI RGATCY 2 cut(s) 364, 1073
MhlI GDGCHC 1 cut(s) 444
MluCI AATT 7 cut(s) 85, 226, 316, 325, 1009, 1027, 1047
MlyI GAGTC 3 cut(s) 155, 401, 938
MroXI GAANNNNTTC 1 cut(s) 240
MseI TTAA 5 cut(s) 125, 252, 329, 410, 1207
MslI CAYNNNNRTG 1 cut(s) 1175
MspA1I CMGCKG 2 cut(s) 731, 860
MspI CCGG 3 cut(s) 78, 101, 105
MspR9I CCNGG 3 cut(s) 60, 453, 1109
Mva1269I GAATGC 1 cut(s) 1161
MvaI CCWGG 3 cut(s) 60, 453, 1109
MwoI GCNNNNNNNGC 3 cut(s) 737, 746, 1104
NlaIII CATG 4 cut(s) 266, 400, 1174, 1180
NlaIV GGNNCC 1 cut(s) 348
NmuCI GTSAC 2 cut(s) 138, 618
PciSI GCTCTTC 1 cut(s) 1197
PctI GAATGC 1 cut(s) 1161
PdmI GAANNNNTTC 1 cut(s) 240
PfeI GAWTC 2 cut(s) 580, 806
PfoI TCCNGGA 1 cut(s) 58
PkrI GCNGC 4 cut(s) 742, 763, 862, 1068
PleI GAGTC 3 cut(s) 154, 400, 937
PpsI GAGTC 3 cut(s) 154, 400, 937
Psp6I CCWGG 3 cut(s) 58, 451, 1107
PspGI CCWGG 3 cut(s) 58, 451, 1107
PspN4I GGNNCC 1 cut(s) 348
PsuI RGATCY 2 cut(s) 364, 1073
PvuII CAGCTG 2 cut(s) 731, 860
RsaI GTAC 2 cut(s) 76, 557
RsaNI GTAC 2 cut(s) 75, 556
RseI CAYNNNNRTG 1 cut(s) 1175
SapI GCTCTTC 1 cut(s) 1197
SaqAI TTAA 5 cut(s) 125, 252, 329, 410, 1207
SatI GCNGC 4 cut(s) 741, 762, 861, 1067
SchI GAGTC 3 cut(s) 155, 401, 938
ScrFI CCNGG 3 cut(s) 60, 453, 1109
SduI GDGCHC 1 cut(s) 444
SfaNI GCATC 2 cut(s) 1126, 1177
SfcI CTRYAG 1 cut(s) 855
SmiMI CAYNNNNRTG 1 cut(s) 1175
SmlI CTYRAG 2 cut(s) 437, 821
SmoI CTYRAG 2 cut(s) 437, 821
Sse9I AATT 7 cut(s) 85, 226, 316, 325, 1009, 1027, 1047
SspI AATATT 1 cut(s) 922
SspMI CTAG 3 cut(s) 150, 488, 887
StyD4I CCNGG 3 cut(s) 58, 451, 1107
TaaI ACNGT 3 cut(s) 138, 446, 560
TaqI TCGA 2 cut(s) 83, 723
TasI AATT 7 cut(s) 85, 226, 316, 325, 1009, 1027, 1047
TatI WGTACW 1 cut(s) 555
TfiI GAWTC 2 cut(s) 580, 806
Tru1I TTAA 5 cut(s) 125, 252, 329, 410, 1207
Tru9I TTAA 5 cut(s) 125, 252, 329, 410, 1207
TscAI CASTG 4 cut(s) 141, 449, 565, 763
TseFI GTSAC 2 cut(s) 138, 618
TseI GCWGC 4 cut(s) 740, 761, 860, 1066
Tsp45I GTSAC 2 cut(s) 138, 618
TspDTI ATGAA 3 cut(s) 22, 566, 930
TspRI CASTG 4 cut(s) 141, 449, 565, 763
XapI RAATTY 5 cut(s) 85, 226, 316, 325, 1009
XmnI GAANNNNTTC 1 cut(s) 240
XspI CTAG 3 cut(s) 150, 488, 887
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.