Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr04

Orthogroup ID

OG0001482

Physical Location & Seq

Genomic Coordinates Reverse (-)

1105736 .. 1109257

Gene Structure 3522 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

MD04G1009800.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1635 bp

ATGGGTTTGTTTTTGTTTCCACCGCCCAAAATTGCAACCTTTAATCTGCTGCTTGTGCTGACTGTGAGTCCCGTGAGCTTCACGTTTGTCAATGGAGGAATACCGACAACCCTCGAAGGACCCTTTAAGCCTGTCACTGTTCCTCTCGACGACAGCTTTCGAGGCAATGCCGTTGAATTGCCGGACACTGATCCTCGAGTTCGAAGAATCGTCAAGGGTTTCGAGCCTGAACAAATCTCTGTCTCTCTCTCTACAACCTATGACTCTGTCTGGATATCTTGGATTACAGGGGAATTTCAAATTGGAGACAATATAAAACCGTTGGATCCGAATAATGTTTCAAGTATTGTTACATATGGAAGGTACGGATTCCCTATGGATAACCAATCAACGGGGTATTCCCTTATTTACAATCAGCTGTATCCGTTTGAAGGCCTCCAAAACTACACTTCCGGAATTATACACCATGTTCGTCTCACAGGATTGAGACCCAACACTTTGTATCAGTATCAATGTGGAGATCCTAACATTGCAGATATGAGCAAAATATCTTATTTCAAGACTATGCCAGTATCCGGTCCCAAGAGTTACCCCAGAAGAATAGCTGTGGTTGGGGACCTTGGCCTTACATACAATACTACATCAACGGTCGACCACTTGTTGGCTAACGGACCTGATCTTATGTTATTGGTTGGGGACGTTACTTATGCTAACACGTACCTCACAAATGGCACTGGGTCTGATTGCTACTCTTGCTTATTCTCGCAAACTCCTATTGATGAAACTTATCAGCCTCGTTGGGATTACTGGGGAAGGTTTATGCAGCCGCTAGTGTCTAAAGTTCCAATGATGGTGGTAGAAGGGAACCATGAGATCGAAGAACAGGCTGGGAATCGAACATTTGCTTCTTATAGTTCGCGATTTGCATTCCCCTCCAACGAGAGTGGATCATCATCTACATTGTTTTATTCATTCAATGCAGGCGGGATACACTTTATAATGCTCGGTGCTTACACAGCCTATCACAAATCAGACGATCAATACAAGTGGTTGGAGAAAGACTTGGCTAAAGTTGACAGAAATGTGACTCCTTGGTTGGTAGCAGCATGGCACCCACCATGGTACAGCACCTACACGGCACATTACAGAGAAGAGGAATGTATGAAAGTTGAGATGGAAGACATACTGTACAAGTATGGTGTTGACATCGTCTTCAACGGCCATGTTCACGCCTACGAGAGGTCAAATCGAGTGTATAACTACACATTGGATCCCTGCGGCCCTGTTTACATCACAGTTGGTGATGGTGGTAACCGGGAGAAGATGGCAATTGCGCATGCTGATGAACCCGGAAACTGCCCAGAACCATCTACAACTTCGGATAAGTTTATGGGCGGCTTCTGCGCATTTAATTTCACAAATGGCCCAGCAGCAGGAAAGTTCTGTTGGGACAAGCAGCCTGAATACAGTGCGTATCGAGAGAGTAGCTTTGGCCATGGGATTCTAGAGGTGAAAAACGAGACTTATGCTTTATGGACTTGGCACCGGAATCAGGACATGTACAGCACCTCTGCAGACCTAATTTACATTGTAAGGCAGCCCGATAGGTGTCCGGTTACAGACAACGGTGCATAA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000003 GO:0003674 GO:0003824 GO:0003993 GO:0006793 GO:0006796 GO:0007275 GO:0008150 GO:0008152 GO:0009791 GO:0009845 GO:0009846 GO:0009856 GO:0009987 GO:0016311 GO:0016787 GO:0016788 GO:0016791 GO:0022414 GO:0032501 GO:0032502 GO:0042578 GO:0044237 GO:0044706 GO:0048856 GO:0051704 GO:0090351

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

Metallophos Metallophos_C Pur_ac_phosph_N

Protein Analysis

545

Amino Acids

61.29

Weight (kDa)

5.21

Isoelectric Point (pI)

34.33

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Pur_ac_phosph_N	PF16656	76 - 188	2.9e-14	Purple acid Phosphatase, N-terminal domain
Metallophos	PF00149	200 - 412	5.6e-22	Calcineurin-like phosphoesterase
Metallophos_C	PF14008	480 - 519	6.9e-10	Iron/zinc purple acid phosphatase-like protein C

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0001482)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT3G07130 AT3G07130
fragaria_vesca	FvH4_2g18800 FvH4_2g18800 FvH4_2g18800 FvH4_2g18800 FvH4_2g18800 FvH4_4g16520 FvH4_4g16520 FvH4_4g16520 FvH4_4g16520 FvH4_4g16520 FvH4_4g16520 FvH4_4g16520 FvH4_4g16520
malus_domestica	MD04G1009700.v1.1 MD04G1009800.v1.1 MD05G1174000.v1.1 MD10G1161900.v1.1
prunus_persica	Prupe.1G008900_v2.0.a1 Prupe.1G008900_v2.0.a1 Prupe.1G008900_v2.0.a1 Prupe.1G008900_v2.0.a1 Prupe.1G008900_v2.0.a1 Prupe.8G201400_v2.0.a1
pyrus_communis	pycom04g00750 pycom04g00760 pycom05g16070 pycom10g13990
rosa_chinensis	RchiOBHm_Chr4g0415071 RchiOBHm_Chr6g0284551
rosa_laevigata	RLG00000008098 RLG00000012739
rosa_multiflora	Rmu_co8519847.1_g000001 Rmu_sc0001246.1_g000001 Rmu_sc0005257.1_g000004 Rmu_sc0042686.1_g000001
rosa_roxburghii	Rroxscaffold_5G00358850
rosa_rugosa	Rorug04G0132200 Rorug04G0132300 Rorug06G0164100
rosa_samantha	Rh4AG191700 Rh4BG189600 Rh4CG202400 Rh4DG190000 Rh6AG277300 Rh6BG277200 Rh6CG279400 Rh6DG271800
rosa_wichuraiana	Rw4G016280 Rw6G023810

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	1 cut(s)	998
Acc16I	TGCGCA	2 cut(s)	1335, 1405
AccB1I	GGYRCC	2 cut(s)	1110, 1542
AccB7I	CCANNNNNTGG	1 cut(s)	661
AccI	GTMKAC	1 cut(s)	651
AccII	CGCG	1 cut(s)	919
AccIII	TCCGGA	1 cut(s)	452
AciI	CCGC	5 cut(s)	23, 827, 984, 1278, 1395
AclWI	GGATC	7 cut(s)	185, 320, 333, 515, 955, 1265, 1278
AcoI	YGGCCR	2 cut(s)	1219, 1492
AcsI	RAATTY	1 cut(s)	293
AfaI	GTAC	5 cut(s)	365, 719, 1124, 1190, 1562
AfiI	CCNNNNNNNGG	7 cut(s)	391, 431, 575, 661, 1239, 1433, 1552
AflIII	ACRYGT	2 cut(s)	714, 1557
AgsI	TTSAA	7 cut(s)	176, 299, 342, 431, 559, 976, 1216
AhdI	GACNNNNNGTC	1 cut(s)	66
AjuI	GAANNNNNNNTTGG	4 cut(s)	285, 317, 1429, 1461
AluBI	AGCT	5 cut(s)	78, 156, 418, 605, 1488
AluI	AGCT	5 cut(s)	78, 156, 418, 605, 1488
Alw26I	GTCTC	5 cut(s)	247, 300, 479, 481, 1514
AlwI	GGATC	7 cut(s)	185, 320, 333, 515, 955, 1265, 1278
Ama87I	CYCGRG	1 cut(s)	195
Aor13HI	TCCGGA	1 cut(s)	452
AoxI	GGCC	6 cut(s)	433, 622, 1219, 1279, 1423, 1492
ApeKI	GCWGC	6 cut(s)	49, 823, 1103, 1430, 1456, 1597
ApoI	RAATTY	1 cut(s)	293
AspLEI	GCGC	2 cut(s)	1336, 1406
AspS9I	GGNCC	6 cut(s)	119, 578, 616, 671, 1280, 1424
AsuC2I	CCSGG	2 cut(s)	1316, 1350
AsuHPI	GGTGA	2 cut(s)	1313, 1522
AsuII	TTCGAA	1 cut(s)	202
AvaI	CYCGRG	1 cut(s)	195
AvaII	GGWCC	4 cut(s)	119, 578, 616, 671
BalI	TGGCCA	1 cut(s)	1494
BamHI	GGATCC	2 cut(s)	325, 1270
BanI	GGYRCC	2 cut(s)	1110, 1542
BbsI	GAAGAC	2 cut(s)	1185, 1204
BbvI	GCAGC	6 cut(s)	36, 835, 1115, 1442, 1468, 1609
BccI	CCATC	5 cut(s)	844, 1168, 1298, 1318, 1375
BceAI	ACGGC	3 cut(s)	155, 1152, 1234
BcgI	CGANNNNNNTGC	2 cut(s)	1508, 1542
BciVI	GTATCC	3 cut(s)	432, 583, 981
BcnI	CCSGG	2 cut(s)	1316, 1350
BcoDI	GTCTC	5 cut(s)	247, 300, 479, 481, 1514
BfaI	CTAG	2 cut(s)	830, 1505
BfmI	CTRYAG	1 cut(s)	1572
BfuI	GTATCC	3 cut(s)	432, 583, 981
BisI	GCNGC	9 cut(s)	50, 824, 827, 1104, 1279, 1396, 1431, 1457, 1598
BlsI	GCNGC	9 cut(s)	51, 825, 828, 1105, 1280, 1397, 1432, 1458, 1599
Bme1390I	CCNGG	2 cut(s)	1316, 1350
Bme18I	GGWCC	4 cut(s)	119, 578, 616, 671
BmeRI	GACNNNNNGTC	1 cut(s)	66
BmeT110I	CYCGRG	1 cut(s)	195
BmgT120I	GGNCC	6 cut(s)	119, 578, 616, 671, 1280, 1424
BmiI	GGNNCC	8 cut(s)	121, 327, 580, 617, 866, 1112, 1272, 1544
BmrFI	CCNGG	2 cut(s)	1316, 1350
BmrI	ACTGGG	2 cut(s)	744, 817
BmuI	ACTGGG	2 cut(s)	744, 817
BpiI	GAAGAC	2 cut(s)	1185, 1204
Bpu14I	TTCGAA	1 cut(s)	202
BpuMI	CCSGG	2 cut(s)	1316, 1350
BsaAI	YACGTR	1 cut(s)	717
BsaBI	GATNNNNATC	1 cut(s)	952
BsaI	GGTCTC	1 cut(s)	481
BsaJI	CCNNGG	4 cut(s)	619, 1091, 1118, 1495
BsaWI	WCCGGW	4 cut(s)	452, 575, 1545, 1612
Bsc4I	CCNNNNNNNGG	7 cut(s)	391, 431, 575, 661, 1239, 1433, 1552
Bse1I	ACTGG	3 cut(s)	569, 739, 812
Bse3DI	GCAATG	2 cut(s)	172, 528
Bse8I	GATNNNNATC	1 cut(s)	952
BseAI	TCCGGA	1 cut(s)	452
BseDI	CCNNGG	4 cut(s)	619, 1091, 1118, 1495
BseJI	GATNNNNATC	1 cut(s)	952
BseLI	CCNNNNNNNGG	7 cut(s)	391, 431, 575, 661, 1239, 1433, 1552
BseMI	GCAATG	2 cut(s)	172, 528
BseNI	ACTGG	3 cut(s)	569, 739, 812
BseXI	GCAGC	6 cut(s)	36, 835, 1115, 1442, 1468, 1609
BseYI	CCCAGC	2 cut(s)	887, 1426
Bsh1236I	CGCG	1 cut(s)	919
Bsh1285I	CGRYCG	1 cut(s)	651
BshFI	GGCC	6 cut(s)	435, 624, 1221, 1281, 1425, 1494
BshNI	GGYRCC	2 cut(s)	1110, 1542
BsiEI	CGRYCG	1 cut(s)	651
BsiHKCI	CYCGRG	1 cut(s)	195
BsiSI	CCGG	7 cut(s)	182, 453, 576, 1315, 1350, 1546, 1613
BslFI	GGGAC	5 cut(s)	54, 564, 629, 710, 1463
BslI	CCNNNNNNNGG	7 cut(s)	391, 431, 575, 661, 1239, 1433, 1552
BsmAI	GTCTC	5 cut(s)	247, 300, 479, 481, 1514
BsmBI	CGTCTC	1 cut(s)	479
BsmFI	GGGAC	5 cut(s)	54, 564, 629, 710, 1463
BsmI	GAATGC	1 cut(s)	926
BsnI	GGCC	6 cut(s)	435, 624, 1221, 1281, 1425, 1494
Bso31I	GGTCTC	1 cut(s)	481
BsoBI	CYCGRG	1 cut(s)	195
Bsp119I	TTCGAA	1 cut(s)	202
Bsp13I	TCCGGA	1 cut(s)	452
Bsp1407I	TGTACA	2 cut(s)	1188, 1560
Bsp143I	GATC	8 cut(s)	190, 325, 520, 676, 873, 947, 1036, 1270
Bsp19I	CCATGG	2 cut(s)	1118, 1495
Bsp68I	TCGCGA	1 cut(s)	919
BspACI	CCGC	5 cut(s)	23, 827, 984, 1278, 1395
BspANI	GGCC	6 cut(s)	435, 624, 1221, 1281, 1425, 1494
BspEI	TCCGGA	1 cut(s)	452
BspFNI	CGCG	1 cut(s)	919
BspLI	GGNNCC	8 cut(s)	121, 327, 580, 617, 866, 1112, 1272, 1544
BspMAI	CTGCAG	1 cut(s)	1576
BspPI	GGATC	7 cut(s)	185, 320, 333, 515, 955, 1265, 1278
BspT104I	TTCGAA	1 cut(s)	202
BspT107I	GGYRCC	2 cut(s)	1110, 1542
BspTNI	GGTCTC	1 cut(s)	481
BsrDI	GCAATG	2 cut(s)	172, 528
BsrGI	TGTACA	2 cut(s)	1188, 1560
BsrI	ACTGG	3 cut(s)	569, 739, 812
BssECI	CCNNGG	4 cut(s)	619, 1091, 1118, 1495
BssMI	GATC	8 cut(s)	190, 325, 520, 676, 873, 947, 1036, 1270
BssT1I	CCWWGG	4 cut(s)	619, 1091, 1118, 1495
Bst4CI	ACNGT	8 cut(s)	64, 139, 321, 649, 1188, 1297, 1469, 1628
Bst6I	CTCTTC	1 cut(s)	1146
BstAUI	TGTACA	2 cut(s)	1188, 1560
BstBAI	YACGTR	1 cut(s)	717
BstBI	TTCGAA	1 cut(s)	202
BstC8I	GCNNGC	2 cut(s)	982, 1338
BstDSI	CCRYGG	2 cut(s)	1118, 1495
BstEII	GGTNACC	1 cut(s)	1310
BstENI	CCTNNNNNAGG	1 cut(s)	1237
BstFNI	CGCG	1 cut(s)	919
BstHHI	GCGC	2 cut(s)	1336, 1406
BstKTI	GATC	8 cut(s)	193, 328, 523, 679, 876, 950, 1039, 1273
BstMAI	GTCTC	5 cut(s)	247, 300, 479, 481, 1514
BstMBI	GATC	8 cut(s)	190, 325, 520, 676, 873, 947, 1036, 1270
BstMCI	CGRYCG	1 cut(s)	651
BstMWI	GCNNNNNNNGC	5 cut(s)	55, 162, 753, 1016, 1401
BstNSI	RCATGY	2 cut(s)	1340, 1561
BstPI	GGTNACC	1 cut(s)	1310
BstSCI	CCNGG	2 cut(s)	1314, 1348
BstSFI	CTRYAG	1 cut(s)	1572
BstUI	CGCG	1 cut(s)	919
BstV1I	GCAGC	6 cut(s)	36, 835, 1115, 1442, 1468, 1609
BstV2I	GAAGAC	2 cut(s)	1185, 1204
BstX2I	RGATCY	3 cut(s)	325, 520, 1270
BstYI	RGATCY	3 cut(s)	325, 520, 1270
BsuI	GTATCC	3 cut(s)	432, 583, 981
BsuRI	GGCC	6 cut(s)	435, 624, 1221, 1281, 1425, 1494
BtgI	CCRYGG	2 cut(s)	1118, 1495
BtsIMutI	CAGTG	4 cut(s)	135, 186, 732, 1474
BtuMI	TCGCGA	1 cut(s)	919
Cac8I	GCNNGC	2 cut(s)	982, 1338
CfoI	GCGC	2 cut(s)	1336, 1406
Cfr13I	GGNCC	6 cut(s)	119, 578, 616, 671, 1280, 1424
Csp6I	GTAC	5 cut(s)	364, 718, 1123, 1189, 1561
CspCI	CAANNNNNGTGG	6 cut(s)	834, 869, 925, 960, 1028, 1063
CviAII	CATG	8 cut(s)	467, 869, 1107, 1119, 1223, 1337, 1496, 1558
CviQI	GTAC	5 cut(s)	364, 718, 1123, 1189, 1561
DpnI	GATC	8 cut(s)	192, 327, 522, 678, 875, 949, 1038, 1272
DpnII	GATC	8 cut(s)	190, 325, 520, 676, 873, 947, 1036, 1270
DriI	GACNNNNNGTC	1 cut(s)	66
EaeI	YGGCCR	2 cut(s)	1219, 1492
Eam1104I	CTCTTC	1 cut(s)	1146
Eam1105I	GACNNNNNGTC	1 cut(s)	66
EarI	CTCTTC	1 cut(s)	1146
Eco130I	CCWWGG	4 cut(s)	619, 1091, 1118, 1495
Eco147I	AGGCCT	1 cut(s)	435
Eco31I	GGTCTC	1 cut(s)	481
Eco32I	GATATC	1 cut(s)	276
Eco47I	GGWCC	4 cut(s)	119, 578, 616, 671
Eco88I	CYCGRG	1 cut(s)	195
Eco91I	GGTNACC	1 cut(s)	1310
EcoNI	CCTNNNNNAGG	1 cut(s)	1237
EcoO109I	RGGNCCY	2 cut(s)	119, 616
EcoO65I	GGTNACC	1 cut(s)	1310
EcoRV	GATATC	1 cut(s)	276
EcoT14I	CCWWGG	4 cut(s)	619, 1091, 1118, 1495
ErhI	CCWWGG	4 cut(s)	619, 1091, 1118, 1495
Esp3I	CGTCTC	1 cut(s)	479
FaeI	CATG	8 cut(s)	470, 872, 1110, 1122, 1226, 1340, 1499, 1561
FaqI	GGGAC	5 cut(s)	54, 564, 629, 710, 1463
FatI	CATG	8 cut(s)	466, 868, 1106, 1118, 1222, 1336, 1495, 1557
FauI	CCCGC	1 cut(s)	977
FauNDI	CATATG	1 cut(s)	355
FblI	GTMKAC	1 cut(s)	651
Fnu4HI	GCNGC	9 cut(s)	50, 824, 827, 1104, 1279, 1396, 1431, 1457, 1598
Fsp4HI	GCNGC	9 cut(s)	50, 824, 827, 1104, 1279, 1396, 1431, 1457, 1598
FspBI	CTAG	2 cut(s)	830, 1505
FspI	TGCGCA	2 cut(s)	1335, 1405
GlaI	GCGC	2 cut(s)	1335, 1405
GluI	GCNGC	9 cut(s)	50, 824, 827, 1104, 1279, 1396, 1431, 1457, 1598
GsaI	CCCAGC	2 cut(s)	891, 1430
HaeIII	GGCC	6 cut(s)	435, 624, 1221, 1281, 1425, 1494
HapII	CCGG	7 cut(s)	182, 453, 576, 1315, 1350, 1546, 1613
HhaI	GCGC	2 cut(s)	1336, 1406
Hin1II	CATG	8 cut(s)	470, 872, 1110, 1122, 1226, 1340, 1499, 1561
Hin6I	GCGC	2 cut(s)	1334, 1404
HinP1I	GCGC	2 cut(s)	1334, 1404
HincII	GTYRAC	3 cut(s)	652, 1075, 1204
HindII	GTYRAC	3 cut(s)	652, 1075, 1204
HinfI	GANTC	8 cut(s)	67, 207, 263, 369, 892, 1087, 1501, 1549
HpaII	CCGG	7 cut(s)	182, 453, 576, 1315, 1350, 1546, 1613
HphI	GGTGA	2 cut(s)	1313, 1522
Hpy166II	GTNNAC	5 cut(s)	652, 1075, 1204, 1228, 1288
Hpy188I	TCNGA	4 cut(s)	330, 742, 1033, 1381
Hpy188III	TCNNGA	8 cut(s)	146, 271, 453, 559, 918, 1478, 1505, 1553
Hpy8I	GTNNAC	5 cut(s)	652, 1075, 1204, 1228, 1288
Hpy99I	CGWCG	1 cut(s)	152
HpyAV	CCTTC	5 cut(s)	110, 354, 425, 807, 854
HpyCH4III	ACNGT	8 cut(s)	64, 139, 321, 649, 1188, 1297, 1469, 1628
HpyCH4IV	ACGT	3 cut(s)	83, 699, 716
HpyCH4V	TGCA	7 cut(s)	35, 533, 823, 926, 980, 1574, 1631
HpyF10VI	GCNNNNNNNGC	5 cut(s)	55, 162, 753, 1016, 1401
HpySE526I	ACGT	3 cut(s)	83, 699, 716
Hsp92II	CATG	8 cut(s)	470, 872, 1110, 1122, 1226, 1340, 1499, 1561
HspAI	GCGC	2 cut(s)	1334, 1404
Kpn2I	TCCGGA	1 cut(s)	452
Kzo9I	GATC	8 cut(s)	190, 325, 520, 676, 873, 947, 1036, 1270
Lsp1109I	GCAGC	6 cut(s)	36, 835, 1115, 1442, 1468, 1609
MaeI	CTAG	2 cut(s)	830, 1505
MaeII	ACGT	3 cut(s)	83, 699, 716
MaeIII	GTNAC	7 cut(s)	133, 349, 587, 700, 1084, 1310, 1615
MalI	GATC	8 cut(s)	192, 327, 522, 678, 875, 949, 1038, 1272
MboI	GATC	8 cut(s)	190, 325, 520, 676, 873, 947, 1036, 1270
MboII	GAAGA	7 cut(s)	216, 609, 890, 1163, 1190, 1204, 1333
MfeI	CAATTG	1 cut(s)	1329
MflI	RGATCY	3 cut(s)	325, 520, 1270
MlsI	TGGCCA	1 cut(s)	1494
MluCI	AATT	8 cut(s)	30, 176, 293, 300, 456, 1329, 1411, 1581
MluNI	TGGCCA	1 cut(s)	1494
MlyI	GAGTC	3 cut(s)	76, 257, 1081
MmeI	TCCRAC	3 cut(s)	303, 960, 1032
Mox20I	TGGCCA	1 cut(s)	1494
MroI	TCCGGA	1 cut(s)	452
MscI	TGGCCA	1 cut(s)	1494
MseI	TTAA	3 cut(s)	42, 126, 1410
MslI	CAYNNNNRTG	1 cut(s)	1341
Msp20I	TGGCCA	1 cut(s)	1494
MspA1I	CMGCKG	1 cut(s)	418
MspI	CCGG	7 cut(s)	182, 453, 576, 1315, 1350, 1546, 1613
MspR9I	CCNGG	2 cut(s)	1316, 1350
MunI	CAATTG	1 cut(s)	1329
Mva1269I	GAATGC	1 cut(s)	926
MvnI	CGCG	1 cut(s)	919
MwoI	GCNNNNNNNGC	5 cut(s)	55, 162, 753, 1016, 1401
NciI	CCSGG	2 cut(s)	1316, 1350
NcoI	CCATGG	2 cut(s)	1118, 1495
NdeI	CATATG	1 cut(s)	355
NdeII	GATC	8 cut(s)	190, 325, 520, 676, 873, 947, 1036, 1270
NlaIII	CATG	8 cut(s)	470, 872, 1110, 1122, 1226, 1340, 1499, 1561
NlaIV	GGNNCC	8 cut(s)	121, 327, 580, 617, 866, 1112, 1272, 1544
NmuCI	GTSAC	2 cut(s)	133, 1084
NruI	TCGCGA	1 cut(s)	919
NsbI	TGCGCA	2 cut(s)	1335, 1405
NspI	RCATGY	2 cut(s)	1340, 1561
NspV	TTCGAA	1 cut(s)	202
PaeI	GCATGC	1 cut(s)	1340
PaeR7I	CTCGAG	1 cut(s)	195
PceI	AGGCCT	1 cut(s)	435
PciI	ACATGT	1 cut(s)	1557
PctI	GAATGC	1 cut(s)	926
PfeI	GAWTC	5 cut(s)	207, 369, 892, 1501, 1549
PflFI	GACNNNGTC	2 cut(s)	266, 1208
PflMI	CCANNNNNTGG	1 cut(s)	661
PkrI	GCNGC	9 cut(s)	51, 825, 828, 1105, 1280, 1397, 1432, 1458, 1599
PleI	GAGTC	3 cut(s)	75, 257, 1081
PpsI	GAGTC	3 cut(s)	75, 257, 1081
Ppu21I	YACGTR	1 cut(s)	717
PpuMI	RGGWCCY	2 cut(s)	119, 616
PscI	ACATGT	1 cut(s)	1557
PsiI	TTATAA	1 cut(s)	998
Psp5II	RGGWCCY	2 cut(s)	119, 616
PspEI	GGTNACC	1 cut(s)	1310
PspFI	CCCAGC	2 cut(s)	887, 1426
PspN4I	GGNNCC	8 cut(s)	121, 327, 580, 617, 866, 1112, 1272, 1544
PspPI	GGNCC	6 cut(s)	119, 578, 616, 671, 1280, 1424
PspPPI	RGGWCCY	2 cut(s)	119, 616
PspXI	VCTCGAGB	1 cut(s)	195
PstI	CTGCAG	1 cut(s)	1576
PsuI	RGATCY	3 cut(s)	325, 520, 1270
PsyI	GACNNNGTC	2 cut(s)	266, 1208
PvuII	CAGCTG	1 cut(s)	418
RruI	TCGCGA	1 cut(s)	919
RsaI	GTAC	5 cut(s)	365, 719, 1124, 1190, 1562
RsaNI	GTAC	5 cut(s)	364, 718, 1123, 1189, 1561
RseI	CAYNNNNRTG	1 cut(s)	1341
SalI	GTCGAC	1 cut(s)	650
SaqAI	TTAA	3 cut(s)	42, 126, 1410
SatI	GCNGC	9 cut(s)	50, 824, 827, 1104, 1279, 1396, 1431, 1457, 1598
Sau3AI	GATC	8 cut(s)	190, 325, 520, 676, 873, 947, 1036, 1270
Sau96I	GGNCC	6 cut(s)	119, 578, 616, 671, 1280, 1424
SchI	GAGTC	3 cut(s)	76, 257, 1081
ScrFI	CCNGG	2 cut(s)	1316, 1350
SfcI	CTRYAG	1 cut(s)	1572
Sfr274I	CTCGAG	1 cut(s)	195
SfuI	TTCGAA	1 cut(s)	202
SinI	GGWCC	4 cut(s)	119, 578, 616, 671
SlaI	CTCGAG	1 cut(s)	195
SmiMI	CAYNNNNRTG	1 cut(s)	1341
SmlI	CTYRAG	1 cut(s)	195
SmoI	CTYRAG	1 cut(s)	195
SphI	GCATGC	1 cut(s)	1340
Sse9I	AATT	8 cut(s)	30, 176, 293, 300, 456, 1329, 1411, 1581
SseBI	AGGCCT	1 cut(s)	435
SsiI	CCGC	5 cut(s)	23, 827, 984, 1278, 1395
SspMI	CTAG	2 cut(s)	830, 1505
StuI	AGGCCT	1 cut(s)	435
StyD4I	CCNGG	2 cut(s)	1314, 1348
StyI	CCWWGG	4 cut(s)	619, 1091, 1118, 1495
TaaI	ACNGT	8 cut(s)	64, 139, 321, 649, 1188, 1297, 1469, 1628
TaiI	ACGT	3 cut(s)	86, 702, 719
TasI	AATT	8 cut(s)	30, 176, 293, 300, 456, 1329, 1411, 1581
TatI	WGTACW	2 cut(s)	1188, 1560
TauI	GCSGC	3 cut(s)	829, 1281, 1398
TfiI	GAWTC	5 cut(s)	207, 369, 892, 1501, 1549
Tru1I	TTAA	3 cut(s)	42, 126, 1410
Tru9I	TTAA	3 cut(s)	42, 126, 1410
TscAI	CASTG	4 cut(s)	142, 193, 739, 1474
TseFI	GTSAC	2 cut(s)	133, 1084
TseI	GCWGC	6 cut(s)	49, 823, 1103, 1430, 1456, 1597
Tsp45I	GTSAC	2 cut(s)	133, 1084
TspDTI	ATGAA	4 cut(s)	795, 960, 1178, 1359
TspGWI	ACGGA	3 cut(s)	381, 414, 684
TspRI	CASTG	4 cut(s)	142, 193, 739, 1474
Tth111I	GACNNNGTC	2 cut(s)	266, 1208
Van91I	CCANNNNNTGG	1 cut(s)	661
VpaK11BI	GGWCC	4 cut(s)	119, 578, 616, 671
XagI	CCTNNNNNAGG	1 cut(s)	1237
XapI	RAATTY	1 cut(s)	293
XbaI	TCTAGA	1 cut(s)	1504
XceI	RCATGY	2 cut(s)	1340, 1561
XhoI	CTCGAG	1 cut(s)	195
XmiI	GTMKAC	1 cut(s)	651
XspI	CTAG	2 cut(s)	830, 1505

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.