Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr05

Orthogroup ID

OG0001482

Physical Location & Seq

Genomic Coordinates Forward (+)

30226979 .. 30230752

Gene Structure 3774 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

MD05G1174000.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1656 bp

ATGGCATTTTCTCCGTGCTCCTCCGTTCCTGCAGTTGCTGCGATAACGTGTTTGATTGCGGTGGTTGTTGGTTGTTATTGGCCCGTTGAGGCCCGTATCCCGACCACCTTGGACGGCCCGTTCCAGCCCCTCACCGTCCCATTTGATCCCAGCTTGCGCGGCAACGCCGTCGATTTGCCTGATGATGATCACCGGGTCCGTCGCCGGGTTAAAGGGTTTGAGCCCGAGCAGATTTCCGTCTCCCTCTCCGCCGATTACGACTCCGTTTGGATCTCCTGGATAACAGGGGAGTATCAGATTGGAGACAACATAATGCCATTGGACCCCAAAAGTGTGCAAAGCGTTGTTCAATATGGGAAGCTGGGGTATCCACCAACAAATGAAGCCTCAGGATATTCTCTTGTTTACAATCAGCTCTACCCTTTTGAAGGCCTCCAAAATTACACTTCCGGAATCATCCACCATGTTCGTCTCACAGGGTTGAAACCGAATACAGTGTATTTTTATCAATGCGGGGATCCTTCTATACCTTCAATGAGTGAAATCCACCATTTCAGGACCATGCCAGTTACTGGTCCTCGGAGCTACCCGGAGAGAATAGCAGTTGTGGGAGACCTTGGCCTTACTTACAACACAACTGCCACAATAAGTCACTTGACAAGTAACAACCCTGATCTTGTTCTATTGATTGGTGATGTTACATATGCAAACCTCTACCTGACAAATGGAACTGGATCTGACTGTTATTCTTGCTCATTTCCAAACTCTCCGATACATGAGACCTATCAGCCTAGATGGGATTACTGGGGAAGGTATATGCAGAGTTTAGTTTCCAAAGTTCCAATAATGGTGGTCGAAGGGAACCATGAAATAGAAGAACAGGCTGAAAATAAGACATTTGAGGCTTATAGCTCTCGGTTTGCATTCCCATCTGAAGAAAGCGGATCCTCGTCCACATTCTACTACTCATTTAATGCAGGGGGGATTCATTTTATAATGATTGGAGCCTACACTGATTTTAGCAAATCAGGGAAGCAATACAAGTGGCTGGAGCAAGATTTGGCTAATGTGGACAGATCCATAACTCCATGGCTGGTAGCTACTTGGCATCCACCCTGGTATAGTACCTATGAGGCCCATTATAGAGAGGCAGAATGTATGAGGATGGAAATGGAAGAGCTACTGTACTCCTATGGCGTCGATATAGTGTTTAGTGGACATGTTCATGCCTACGAGAGGTCGAATCGAGTTTATGATTACAACTTAGATCCCTGTGGTCCTGTATATCTCACCGTTGGTGATGGGGGTAATCGAGAGAAGATGGCAGTTCATCATGCTGATGAACCTGGTCATTGCCCAGAGCCATTAACTACTCCTGATGAACACCTAGGTGCGGGTGCCTTTTGTGCAATGAACTTTACCTCTGGCCCAGCGGCAGGTAAATTTTGTTGGGATCGGCAACCGGATTACAGTGCTTTCAGAGAAAGCAGCTTTGGCCATGGAATCCTTGAGGTGAAGAATGAGACTTGGGCTTTATGGACATGGTACCGGAACCAGGACTCAGACGATAAAATTGGAGATCAAATTTACATAGTGAGGCAACCAGATAAGTGCCGCTCCCGCTACATGTTGACGAGTTGGATTGCTGAGCTCTAA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000003 GO:0003674 GO:0003824 GO:0003993 GO:0006793 GO:0006796 GO:0007275 GO:0008150 GO:0008152 GO:0009791 GO:0009845 GO:0009846 GO:0009856 GO:0009987 GO:0016311 GO:0016787 GO:0016788 GO:0016791 GO:0022414 GO:0032501 GO:0032502 GO:0042578 GO:0044237 GO:0044706 GO:0048856 GO:0051704 GO:0090351

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

Metallophos Metallophos_C OKR_DC_1 OKR_DC_1_C Pur_ac_phosph_N

Protein Analysis

552

Amino Acids

62.25

Weight (kDa)

5.03

Isoelectric Point (pI)

42.76

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Pur_ac_phosph_N	PF16656	75 - 187	7.8e-18	Purple acid Phosphatase, N-terminal domain
Metallophos	PF00149	199 - 411	1.8e-22	Calcineurin-like phosphoesterase
Metallophos_C	PF14008	481 - 528	5.6e-10	Iron/zinc purple acid phosphatase-like protein C

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

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Align Labels (Cladogram)

Image

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Publication-ready

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Orthologous Genes (Group: OG0001482)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT3G07130 AT3G07130
fragaria_vesca	FvH4_2g18800 FvH4_2g18800 FvH4_2g18800 FvH4_2g18800 FvH4_2g18800 FvH4_4g16520 FvH4_4g16520 FvH4_4g16520 FvH4_4g16520 FvH4_4g16520 FvH4_4g16520 FvH4_4g16520 FvH4_4g16520
malus_domestica	MD04G1009700.v1.1 MD04G1009800.v1.1 MD05G1174000.v1.1 MD10G1161900.v1.1
prunus_persica	Prupe.1G008900_v2.0.a1 Prupe.1G008900_v2.0.a1 Prupe.1G008900_v2.0.a1 Prupe.1G008900_v2.0.a1 Prupe.1G008900_v2.0.a1 Prupe.8G201400_v2.0.a1
pyrus_communis	pycom04g00750 pycom04g00760 pycom05g16070 pycom10g13990
rosa_chinensis	RchiOBHm_Chr4g0415071 RchiOBHm_Chr6g0284551
rosa_laevigata	RLG00000008098 RLG00000012739
rosa_multiflora	Rmu_co8519847.1_g000001 Rmu_sc0001246.1_g000001 Rmu_sc0005257.1_g000004 Rmu_sc0042686.1_g000001
rosa_roxburghii	Rroxscaffold_5G00358850
rosa_rugosa	Rorug04G0132200 Rorug04G0132300 Rorug06G0164100
rosa_samantha	Rh4AG191700 Rh4BG189600 Rh4CG202400 Rh4DG190000 Rh6AG277300 Rh6BG277200 Rh6CG279400 Rh6DG271800
rosa_wichuraiana	Rw4G016280 Rw6G023810

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	1 cut(s)	995
Acc36I	ACCTGC	1 cut(s)	1427
Acc65I	GGTACC	1 cut(s)	1545
AccB1I	GGYRCC	2 cut(s)	1397, 1545
AccB7I	CCANNNNNTGG	1 cut(s)	572
AccBSI	CCGCTC	1 cut(s)	1617
AccII	CGCG	1 cut(s)	159
AccIII	TCCGGA	1 cut(s)	449
AciI	CCGC	9 cut(s)	59, 159, 249, 513, 942, 1394, 1433, 1615, 1621
AcoI	YGGCCR	1 cut(s)	1495
AcsI	RAATTY	2 cut(s)	1442, 1584
AcuI	CTGAAG	1 cut(s)	954
AcyI	GRCGYC	1 cut(s)	1197
AfaI	GTAC	3 cut(s)	1126, 1187, 1547
AfiI	CCNNNNNNNGG	7 cut(s)	205, 428, 572, 1236, 1393, 1436, 1555
AflIII	ACRYGT	3 cut(s)	47, 1219, 1626
AgsI	TTSAA	4 cut(s)	350, 428, 484, 534
AjnI	CCWGG	4 cut(s)	275, 1115, 1345, 1554
AjuI	GAANNNNNNNTTGG	2 cut(s)	1476, 1508
AleI	CACNNNNGTG	1 cut(s)	1389
AloI	GAACNNNNNNTCC	2 cut(s)	104, 136
AluBI	AGCT	9 cut(s)	153, 361, 415, 585, 912, 1100, 1180, 1491, 1651
AluI	AGCT	9 cut(s)	153, 361, 415, 585, 912, 1100, 1180, 1491, 1651
Alw21I	GWGCWC	2 cut(s)	20, 1653
Alw26I	GTCTC	6 cut(s)	244, 297, 476, 606, 773, 1517
AlwNI	CAGNNNCTG	2 cut(s)	38, 572
Ama87I	CYCGRG	1 cut(s)	224
Aor13HI	TCCGGA	1 cut(s)	449
AoxI	GGCC	8 cut(s)	80, 90, 115, 430, 619, 1134, 1426, 1495
ApeKI	GCWGC	2 cut(s)	38, 1488
ApoI	RAATTY	2 cut(s)	1442, 1584
Asp718I	GGTACC	1 cut(s)	1545
AspA2I	CCTAGG	1 cut(s)	1387
AspLEI	GCGC	1 cut(s)	159
AsuC2I	CCSGG	3 cut(s)	194, 206, 590
AsuHPI	GGTGA	6 cut(s)	124, 182, 704, 1282, 1310, 1525
AvaI	CYCGRG	1 cut(s)	224
AvaII	GGWCC	5 cut(s)	196, 322, 558, 575, 1277
AvrII	CCTAGG	1 cut(s)	1387
AxyI	CCTNAGG	1 cut(s)	388
BalI	TGGCCA	1 cut(s)	1497
BamHI	GGATCC	2 cut(s)	517, 944
BanI	GGYRCC	2 cut(s)	1397, 1545
BanII	GRGCYC	2 cut(s)	225, 1653
Bbv12I	GWGCWC	2 cut(s)	20, 1653
BbvI	GCAGC	2 cut(s)	25, 1500
BccI	CCATC	5 cut(s)	789, 937, 1159, 1295, 1315
BceAI	ACGGC	2 cut(s)	130, 152
BcgI	CGANNNNNNTGC	2 cut(s)	151, 185
BciT130I	CCWGG	4 cut(s)	277, 1117, 1347, 1556
BciVI	GTATCC	2 cut(s)	107, 378
BclI	TGATCA	1 cut(s)	187
BcnI	CCSGG	3 cut(s)	194, 206, 590
BcoDI	GTCTC	6 cut(s)	244, 297, 476, 606, 773, 1517
BfaI	CTAG	2 cut(s)	792, 1388
BfmI	CTRYAG	1 cut(s)	30
BfuAI	ACCTGC	1 cut(s)	1427
BfuI	GTATCC	2 cut(s)	107, 378
BisI	GCNGC	5 cut(s)	39, 160, 1434, 1489, 1615
BlnI	CCTAGG	1 cut(s)	1387
BlpI	GCTNAGC	1 cut(s)	1647
BlsI	GCNGC	5 cut(s)	40, 161, 1435, 1490, 1616
Bme1390I	CCNGG	7 cut(s)	194, 206, 277, 590, 1117, 1347, 1556
Bme18I	GGWCC	5 cut(s)	196, 322, 558, 575, 1277
BmeT110I	CYCGRG	1 cut(s)	224
BmiI	GGNNCC	9 cut(s)	197, 324, 519, 863, 946, 1006, 1399, 1547, 1553
BmrFI	CCNGG	7 cut(s)	194, 206, 277, 590, 1117, 1347, 1556
BmrI	ACTGGG	1 cut(s)	814
BmsI	GCATC	1 cut(s)	1117
BmuI	ACTGGG	1 cut(s)	814
BpmI	CTGGAG	1 cut(s)	1070
Bpu1102I	GCTNAGC	1 cut(s)	1647
BpuEI	CTTGAG	1 cut(s)	1529
BpuMI	CCSGG	3 cut(s)	194, 206, 590
BsaBI	GATNNNNATC	1 cut(s)	186
BsaHI	GRCGYC	1 cut(s)	1197
BsaI	GGTCTC	2 cut(s)	606, 773
BsaJI	CCNNGG	7 cut(s)	108, 578, 616, 1088, 1115, 1387, 1498
BsaWI	WCCGGW	3 cut(s)	449, 1462, 1548
Bsc4I	CCNNNNNNNGG	7 cut(s)	205, 428, 572, 1236, 1393, 1436, 1555
Bse1I	ACTGG	4 cut(s)	566, 577, 736, 809
Bse21I	CCTNAGG	1 cut(s)	388
Bse3DI	GCAATG	2 cut(s)	1351, 1416
Bse8I	GATNNNNATC	1 cut(s)	186
BseAI	TCCGGA	1 cut(s)	449
BseBI	CCWGG	4 cut(s)	277, 1117, 1347, 1556
BseDI	CCNNGG	7 cut(s)	108, 578, 616, 1088, 1115, 1387, 1498
BseGI	GGATG	3 cut(s)	456, 1108, 1170
BseJI	GATNNNNATC	1 cut(s)	186
BseLI	CCNNNNNNNGG	7 cut(s)	205, 428, 572, 1236, 1393, 1436, 1555
BseMI	GCAATG	2 cut(s)	1351, 1416
BseMII	CTCAG	3 cut(s)	402, 1575, 1638
BseNI	ACTGG	4 cut(s)	566, 577, 736, 809
BseRI	GAGGAG	1 cut(s)	10
BseXI	GCAGC	2 cut(s)	25, 1500
BseYI	CCCAGC	3 cut(s)	149, 361, 1429
Bsh1236I	CGCG	1 cut(s)	159
BshFI	GGCC	8 cut(s)	82, 92, 117, 432, 621, 1136, 1428, 1497
BshNI	GGYRCC	2 cut(s)	1397, 1545
BsiHKAI	GWGCWC	2 cut(s)	20, 1653
BsiHKCI	CYCGRG	1 cut(s)	224
BsiSI	CCGG	6 cut(s)	193, 205, 450, 590, 1463, 1549
BslFI	GGGAC	1 cut(s)	122
BslI	CCNNNNNNNGG	7 cut(s)	205, 428, 572, 1236, 1393, 1436, 1555
BsmAI	GTCTC	6 cut(s)	244, 297, 476, 606, 773, 1517
BsmBI	CGTCTC	2 cut(s)	244, 476
BsmFI	GGGAC	1 cut(s)	122
BsmI	GAATGC	1 cut(s)	923
BsnI	GGCC	8 cut(s)	82, 92, 117, 432, 621, 1136, 1428, 1497
Bso31I	GGTCTC	2 cut(s)	606, 773
BsoBI	CYCGRG	1 cut(s)	224
Bsp1286I	GDGCHC	3 cut(s)	20, 225, 1653
Bsp13I	TCCGGA	1 cut(s)	449
Bsp1720I	GCTNAGC	1 cut(s)	1647
Bsp19I	CCATGG	2 cut(s)	1088, 1498
BspACI	CCGC	9 cut(s)	59, 159, 249, 513, 942, 1394, 1433, 1615, 1621
BspANI	GGCC	8 cut(s)	82, 92, 117, 432, 621, 1136, 1428, 1497
BspCNI	CTCAG	3 cut(s)	401, 1574, 1639
BspEI	TCCGGA	1 cut(s)	449
BspFNI	CGCG	1 cut(s)	159
BspLI	GGNNCC	9 cut(s)	197, 324, 519, 863, 946, 1006, 1399, 1547, 1553
BspMAI	CTGCAG	1 cut(s)	34
BspMI	ACCTGC	1 cut(s)	1427
BspQI	GCTCTTC	1 cut(s)	1170
BspT107I	GGYRCC	2 cut(s)	1397, 1545
BspTNI	GGTCTC	2 cut(s)	606, 773
BsrBI	CCGCTC	1 cut(s)	1617
BsrDI	GCAATG	2 cut(s)	1351, 1416
BsrI	ACTGG	4 cut(s)	566, 577, 736, 809
BssECI	CCNNGG	7 cut(s)	108, 578, 616, 1088, 1115, 1387, 1498
BssNI	GRCGYC	1 cut(s)	1197
BssT1I	CCWWGG	5 cut(s)	108, 616, 1088, 1387, 1498
Bst2UI	CCWGG	4 cut(s)	277, 1117, 1347, 1556
Bst4CI	ACNGT	6 cut(s)	136, 496, 743, 1185, 1294, 1472
Bst6I	CTCTTC	1 cut(s)	1170
BstACI	GRCGYC	1 cut(s)	1197
BstAPI	GCANNNNNTGC	1 cut(s)	38
BstC8I	GCNNGC	1 cut(s)	155
BstDEI	CTNAG	4 cut(s)	388, 1264, 1561, 1647
BstDSI	CCRYGG	2 cut(s)	1088, 1498
BstENI	CCTNNNNNAGG	2 cut(s)	426, 1234
BstF5I	GGATG	3 cut(s)	456, 1108, 1170
BstFNI	CGCG	1 cut(s)	159
BstHHI	GCGC	1 cut(s)	159
BstMAI	GTCTC	6 cut(s)	244, 297, 476, 606, 773, 1517
BstMWI	GCNNNNNNNGC	5 cut(s)	38, 159, 1406, 1494, 1620
BstNI	CCWGG	4 cut(s)	277, 1117, 1347, 1556
BstNSI	RCATGY	2 cut(s)	1223, 1630
BstSCI	CCNGG	7 cut(s)	192, 204, 275, 588, 1115, 1345, 1554
BstSFI	CTRYAG	1 cut(s)	30
BstUI	CGCG	1 cut(s)	159
BstV1I	GCAGC	2 cut(s)	25, 1500
BstX2I	RGATCY	6 cut(s)	270, 517, 734, 944, 1076, 1267
BstYI	RGATCY	6 cut(s)	270, 517, 734, 944, 1076, 1267
Bsu36I	CCTNAGG	1 cut(s)	388
BsuI	GTATCC	2 cut(s)	107, 378
BsuRI	GGCC	8 cut(s)	82, 92, 117, 432, 621, 1136, 1428, 1497
BtgI	CCRYGG	2 cut(s)	1088, 1498
BtsCI	GGATG	3 cut(s)	456, 1108, 1170
BtsIMutI	CAGTG	3 cut(s)	501, 1011, 1477
BveI	ACCTGC	1 cut(s)	1427
Cac8I	GCNNGC	1 cut(s)	155
CaiI	CAGNNNCTG	2 cut(s)	38, 572
CfoI	GCGC	1 cut(s)	159
CseI	GACGC	1 cut(s)	1186
CsiI	ACCWGGT	1 cut(s)	1345
Csp6I	GTAC	3 cut(s)	1125, 1186, 1546
CspCI	CAANNNNNGTGG	4 cut(s)	831, 866, 1025, 1060
CviQI	GTAC	3 cut(s)	1125, 1186, 1546
DdeI	CTNAG	4 cut(s)	388, 1264, 1561, 1647
EaeI	YGGCCR	1 cut(s)	1495
Eam1104I	CTCTTC	1 cut(s)	1170
EarI	CTCTTC	1 cut(s)	1170
EciI	GGCGGA	1 cut(s)	238
Ecl136II	GAGCTC	1 cut(s)	1651
Eco130I	CCWWGG	5 cut(s)	108, 616, 1088, 1387, 1498
Eco147I	AGGCCT	1 cut(s)	432
Eco24I	GRGCYC	2 cut(s)	225, 1653
Eco31I	GGTCTC	2 cut(s)	606, 773
Eco47I	GGWCC	5 cut(s)	196, 322, 558, 575, 1277
Eco53kI	GAGCTC	1 cut(s)	1651
Eco57I	CTGAAG	1 cut(s)	954
Eco81I	CCTNAGG	1 cut(s)	388
Eco88I	CYCGRG	1 cut(s)	224
EcoICRI	GAGCTC	1 cut(s)	1651
EcoNI	CCTNNNNNAGG	2 cut(s)	426, 1234
EcoRII	CCWGG	4 cut(s)	275, 1115, 1345, 1554
EcoT14I	CCWWGG	5 cut(s)	108, 616, 1088, 1387, 1498
EcoT38I	GRGCYC	2 cut(s)	225, 1653
ErhI	CCWWGG	5 cut(s)	108, 616, 1088, 1387, 1498
Esp3I	CGTCTC	2 cut(s)	244, 476
FaqI	GGGAC	1 cut(s)	122
FauI	CCCGC	3 cut(s)	506, 1387, 1628
FauNDI	CATATG	1 cut(s)	703
FbaI	TGATCA	1 cut(s)	187
Fnu4HI	GCNGC	5 cut(s)	39, 160, 1434, 1489, 1615
FokI	GGATG	3 cut(s)	443, 1095, 1177
FriOI	GRGCYC	2 cut(s)	225, 1653
Fsp4HI	GCNGC	5 cut(s)	39, 160, 1434, 1489, 1615
FspBI	CTAG	2 cut(s)	792, 1388
GlaI	GCGC	1 cut(s)	158
GluI	GCNGC	5 cut(s)	39, 160, 1434, 1489, 1615
GsaI	CCCAGC	3 cut(s)	153, 365, 1433
GsuI	CTGGAG	1 cut(s)	1070
HaeIII	GGCC	8 cut(s)	82, 92, 117, 432, 621, 1136, 1428, 1497
HapII	CCGG	6 cut(s)	193, 205, 450, 590, 1463, 1549
HgaI	GACGC	1 cut(s)	1186
HhaI	GCGC	1 cut(s)	159
Hin1I	GRCGYC	1 cut(s)	1197
Hin6I	GCGC	1 cut(s)	157
HinP1I	GCGC	1 cut(s)	157
HincII	GTYRAC	1 cut(s)	1632
HindII	GTYRAC	1 cut(s)	1632
HinfI	GANTC	6 cut(s)	260, 453, 985, 1243, 1503, 1559
HpaII	CCGG	6 cut(s)	193, 205, 450, 590, 1463, 1549
HphI	GGTGA	6 cut(s)	124, 182, 704, 1282, 1310, 1525
Hpy166II	GTNNAC	5 cut(s)	406, 954, 1072, 1217, 1632
Hpy188I	TCNGA	7 cut(s)	297, 582, 739, 771, 934, 1481, 1564
Hpy188III	TCNNGA	6 cut(s)	100, 390, 450, 556, 1313, 1376
Hpy8I	GTNNAC	5 cut(s)	406, 954, 1072, 1217, 1632
Hpy99I	CGWCG	3 cut(s)	173, 204, 1202
HpyAV	CCTTC	5 cut(s)	422, 531, 540, 804, 851
HpyCH4III	ACNGT	6 cut(s)	136, 496, 743, 1185, 1294, 1472
HpyCH4IV	ACGT	1 cut(s)	47
HpyCH4V	TGCA	7 cut(s)	32, 337, 707, 820, 923, 977, 1409
HpyF10VI	GCNNNNNNNGC	5 cut(s)	38, 159, 1406, 1494, 1620
HpyF3I	CTNAG	4 cut(s)	388, 1264, 1561, 1647
HpySE526I	ACGT	1 cut(s)	47
Hsp92I	GRCGYC	1 cut(s)	1197
HspAI	GCGC	1 cut(s)	157
Kpn2I	TCCGGA	1 cut(s)	449
KpnI	GGTACC	1 cut(s)	1549
Ksp22I	TGATCA	1 cut(s)	187
LguI	GCTCTTC	1 cut(s)	1170
LmnI	GCTCC	5 cut(s)	23, 582, 1004, 1051, 1622
Lsp1109I	GCAGC	2 cut(s)	25, 1500
LweI	GCATC	1 cut(s)	1117
MabI	ACCWGGT	1 cut(s)	1345
MaeI	CTAG	2 cut(s)	792, 1388
MaeII	ACGT	1 cut(s)	47
MaeIII	GTNAC	4 cut(s)	568, 650, 662, 697
MbiI	CCGCTC	1 cut(s)	1617
MboII	GAAGA	5 cut(s)	887, 947, 1187, 1330, 1528
MflI	RGATCY	6 cut(s)	270, 517, 734, 944, 1076, 1267
MhlI	GDGCHC	3 cut(s)	20, 225, 1653
MlsI	TGGCCA	1 cut(s)	1497
MluCI	AATT	4 cut(s)	439, 1442, 1572, 1584
MluNI	TGGCCA	1 cut(s)	1497
MlyI	GAGTC	2 cut(s)	254, 1553
MmeI	TCCRAC	1 cut(s)	1619
Mox20I	TGGCCA	1 cut(s)	1497
MroI	TCCGGA	1 cut(s)	449
MscI	TGGCCA	1 cut(s)	1497
MseI	TTAA	3 cut(s)	210, 972, 1367
MslI	CAYNNNNRTG	3 cut(s)	1224, 1338, 1389
Msp20I	TGGCCA	1 cut(s)	1497
MspA1I	CMGCKG	1 cut(s)	1433
MspI	CCGG	6 cut(s)	193, 205, 450, 590, 1463, 1549
MspR9I	CCNGG	7 cut(s)	194, 206, 277, 590, 1117, 1347, 1556
Mva1269I	GAATGC	1 cut(s)	923
MvaI	CCWGG	4 cut(s)	277, 1117, 1347, 1556
MvnI	CGCG	1 cut(s)	159
MwoI	GCNNNNNNNGC	5 cut(s)	38, 159, 1406, 1494, 1620
NciI	CCSGG	3 cut(s)	194, 206, 590
NcoI	CCATGG	2 cut(s)	1088, 1498
NdeI	CATATG	1 cut(s)	703
NlaIV	GGNNCC	9 cut(s)	197, 324, 519, 863, 946, 1006, 1399, 1547, 1553
NmuCI	GTSAC	1 cut(s)	650
NspI	RCATGY	2 cut(s)	1223, 1630
OliI	CACNNNNGTG	1 cut(s)	1389
PceI	AGGCCT	1 cut(s)	432
PciI	ACATGT	2 cut(s)	1219, 1626
PciSI	GCTCTTC	1 cut(s)	1170
PctI	GAATGC	1 cut(s)	923
PfeI	GAWTC	4 cut(s)	453, 985, 1243, 1503
PflMI	CCANNNNNTGG	1 cut(s)	572
PfoI	TCCNGGA	1 cut(s)	275
PkrI	GCNGC	5 cut(s)	40, 161, 1435, 1490, 1616
PleI	GAGTC	2 cut(s)	254, 1553
PpsI	GAGTC	2 cut(s)	254, 1553
PscI	ACATGT	2 cut(s)	1219, 1626
PsiI	TTATAA	1 cut(s)	995
Psp124BI	GAGCTC	1 cut(s)	1653
Psp6I	CCWGG	4 cut(s)	275, 1115, 1345, 1554
PspFI	CCCAGC	3 cut(s)	149, 361, 1429
PspGI	CCWGG	4 cut(s)	275, 1115, 1345, 1554
PspN4I	GGNNCC	9 cut(s)	197, 324, 519, 863, 946, 1006, 1399, 1547, 1553
PstI	CTGCAG	1 cut(s)	34
PstNI	CAGNNNCTG	2 cut(s)	38, 572
PsuI	RGATCY	6 cut(s)	270, 517, 734, 944, 1076, 1267
RsaI	GTAC	3 cut(s)	1126, 1187, 1547
RsaNI	GTAC	3 cut(s)	1125, 1186, 1546
RseI	CAYNNNNRTG	3 cut(s)	1224, 1338, 1389
SacI	GAGCTC	1 cut(s)	1653
SapI	GCTCTTC	1 cut(s)	1170
SaqAI	TTAA	3 cut(s)	210, 972, 1367
SatI	GCNGC	5 cut(s)	39, 160, 1434, 1489, 1615
SchI	GAGTC	2 cut(s)	254, 1553
ScrFI	CCNGG	7 cut(s)	194, 206, 277, 590, 1117, 1347, 1556
SduI	GDGCHC	3 cut(s)	20, 225, 1653
SexAI	ACCWGGT	1 cut(s)	1345
SfaNI	GCATC	1 cut(s)	1117
SfcI	CTRYAG	1 cut(s)	30
SinI	GGWCC	5 cut(s)	196, 322, 558, 575, 1277
SmiMI	CAYNNNNRTG	3 cut(s)	1224, 1338, 1389
SmlI	CTYRAG	1 cut(s)	1508
SmoI	CTYRAG	1 cut(s)	1508
Sse9I	AATT	4 cut(s)	439, 1442, 1572, 1584
SseBI	AGGCCT	1 cut(s)	432
SsiI	CCGC	9 cut(s)	59, 159, 249, 513, 942, 1394, 1433, 1615, 1621
SspMI	CTAG	2 cut(s)	792, 1388
SstI	GAGCTC	1 cut(s)	1653
StuI	AGGCCT	1 cut(s)	432
StyD4I	CCNGG	7 cut(s)	192, 204, 275, 588, 1115, 1345, 1554
StyI	CCWWGG	5 cut(s)	108, 616, 1088, 1387, 1498
TaaI	ACNGT	6 cut(s)	136, 496, 743, 1185, 1294, 1472
TaiI	ACGT	1 cut(s)	50
TaqI	TCGA	6 cut(s)	171, 855, 1200, 1241, 1246, 1312
TasI	AATT	4 cut(s)	439, 1442, 1572, 1584
TatI	WGTACW	1 cut(s)	1185
TauI	GCSGC	3 cut(s)	162, 1436, 1617
TfiI	GAWTC	4 cut(s)	453, 985, 1243, 1503
Tru1I	TTAA	3 cut(s)	210, 972, 1367
Tru9I	TTAA	3 cut(s)	210, 972, 1367
TscAI	CASTG	3 cut(s)	501, 1018, 1477
TseFI	GTSAC	1 cut(s)	650
TseI	GCWGC	2 cut(s)	38, 1488
Tsp45I	GTSAC	1 cut(s)	650
TspDTI	ATGAA	8 cut(s)	396, 882, 977, 1214, 1319, 1356, 1395, 1427
TspGWI	ACGGA	4 cut(s)	13, 188, 226, 253
TspRI	CASTG	3 cut(s)	501, 1018, 1477
Van91I	CCANNNNNTGG	1 cut(s)	572
VpaK11BI	GGWCC	5 cut(s)	196, 322, 558, 575, 1277
XagI	CCTNNNNNAGG	2 cut(s)	426, 1234
XapI	RAATTY	2 cut(s)	1442, 1584
XceI	RCATGY	2 cut(s)	1223, 1630
XmaJI	CCTAGG	1 cut(s)	1387
XspI	CTAG	2 cut(s)	792, 1388

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.