MD04G1031600.v1.1

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr04
OG0017116
Physical Location & Seq
Reverse (-)
3549541 .. 3549999
459 bp
Loading structure...
UTR
Exon/CDS
Intron
MD04G1031600.v1.1.491

Sequence Viewer

Length: 459 bp
ATGGGATTGGCTGTGGCTGCAGACCATATAAGGTGGAACTTGGGGGCCATCAAGGACAGACTGTTGGAGAAGCTGGCAGCTGCTGCTGTTCCTGCTGATGCTTTGGACAGTGCACGCCACTTCTTAGAGAGTGTCGTCAAAGTTGTCACTGGGGCTGCGCATGGAATCACCAAGGATGCCTTGCACCGGGTCAGAACACATCTTGTTGACATTCTACCCTCCCTGTCTCCCGAAACCACGAACAAGGTCTCTCTATCTCTCATCTCCTTGATACTTAATTACTTACGAGTGTTTTGTCATACCAGCGATGGTGGGAGGGTGAATGAAATCGAAACTAGGACCTCTCTCGAGTTAGAATCATGCGTTATGAATGTTATAGTTACTCTTCATAACAAGAGACTCGCGACTTCAAATAGATGTTCTCTTGCCAAGAACAAATACACATATCGTCGACGGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

153

Amino Acids

16.8

Weight (kDa)

9.66

Isoelectric Point (pI)

46.04

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0017116)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G16730
fragaria_vesca FvH4_6g36360
malus_domestica MD04G1031600.v1.1
pyrus_communis pycom09g08720
rosa_laevigata RLG00000020343
rosa_multiflora Rmu_co8423113.1_g000001
rosa_roxburghii Rroxscaffold_2G00098830
rosa_rugosa Rorug02G0406100 Rorug02G0406100 Rorug02G0406100
rosa_samantha Rh2BG477800 Rh2DG486700
rosa_wichuraiana Rw2G037910

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc16I TGCGCA 1 cut(s) 159
AccI GTMKAC 1 cut(s) 451
AccII CGCG 1 cut(s) 404
AfiI CCNNNNNNNGG 1 cut(s) 186
AgsI TTSAA 1 cut(s) 411
AluBI AGCT 2 cut(s) 73, 80
AluI AGCT 2 cut(s) 73, 80
Alw21I GWGCWC 1 cut(s) 115
Alw26I GTCTC 3 cut(s) 231, 253, 391
Alw44I GTGCAC 1 cut(s) 111
AlwNI CAGNNNCTG 1 cut(s) 83
Ama87I CYCGRG 1 cut(s) 347
AoxI GGCC 1 cut(s) 45
ApaLI GTGCAC 1 cut(s) 111
ApeKI GCWGC 5 cut(s) 17, 77, 80, 83, 155
AspLEI GCGC 1 cut(s) 160
AspS9I GGNCC 2 cut(s) 45, 339
AsuC2I CCSGG 1 cut(s) 188
AsuHPI GGTGA 2 cut(s) 160, 331
AvaI CYCGRG 1 cut(s) 347
AvaII GGWCC 1 cut(s) 339
BaeGI GKGCMC 1 cut(s) 115
Bbv12I GWGCWC 1 cut(s) 115
BbvI GCAGC 5 cut(s) 4, 67, 70, 89, 142
BccI CCATC 2 cut(s) 56, 302
BcnI CCSGG 1 cut(s) 188
BcoDI GTCTC 3 cut(s) 231, 253, 391
BfaI CTAG 1 cut(s) 336
BfmI CTRYAG 1 cut(s) 18
BisI GCNGC 5 cut(s) 18, 78, 81, 84, 156
BlsI GCNGC 5 cut(s) 19, 79, 82, 85, 157
Bme1390I CCNGG 1 cut(s) 188
Bme18I GGWCC 1 cut(s) 339
BmeT110I CYCGRG 1 cut(s) 347
BmgT120I GGNCC 2 cut(s) 45, 339
BmiI GGNNCC 1 cut(s) 46
BmrFI CCNGG 1 cut(s) 188
BmrI ACTGGG 1 cut(s) 159
BmsI GCATC 2 cut(s) 88, 166
BmuI ACTGGG 1 cut(s) 159
BpuMI CCSGG 1 cut(s) 188
BsaI GGTCTC 1 cut(s) 253
BsaJI CCNNGG 1 cut(s) 171
Bsc4I CCNNNNNNNGG 1 cut(s) 186
Bse1I ACTGG 1 cut(s) 154
BseDI CCNNGG 1 cut(s) 171
BseGI GGATG 1 cut(s) 181
BseLI CCNNNNNNNGG 1 cut(s) 186
BseNI ACTGG 1 cut(s) 154
BseSI GKGCMC 1 cut(s) 115
BseXI GCAGC 5 cut(s) 4, 67, 70, 89, 142
Bsh1236I CGCG 1 cut(s) 404
BshFI GGCC 1 cut(s) 47
BsiHKAI GWGCWC 1 cut(s) 115
BsiHKCI CYCGRG 1 cut(s) 347
BsiSI CCGG 1 cut(s) 187
BslI CCNNNNNNNGG 1 cut(s) 186
BsmAI GTCTC 3 cut(s) 231, 253, 391
BsnI GGCC 1 cut(s) 47
Bso31I GGTCTC 1 cut(s) 253
BsoBI CYCGRG 1 cut(s) 347
Bsp1286I GDGCHC 1 cut(s) 115
Bsp68I TCGCGA 1 cut(s) 404
BspANI GGCC 1 cut(s) 47
BspFNI CGCG 1 cut(s) 404
BspLI GGNNCC 1 cut(s) 46
BspMAI CTGCAG 1 cut(s) 22
BspTNI GGTCTC 1 cut(s) 253
BsrI ACTGG 1 cut(s) 154
BssECI CCNNGG 1 cut(s) 171
BssT1I CCWWGG 1 cut(s) 171
Bst4CI ACNGT 3 cut(s) 63, 110, 456
Bst6I CTCTTC 1 cut(s) 390
BstAPI GCANNNNNTGC 1 cut(s) 83
BstC8I GCNNGC 2 cut(s) 75, 115
BstDEI CTNAG 1 cut(s) 124
BstF5I GGATG 1 cut(s) 181
BstFNI CGCG 1 cut(s) 404
BstHHI GCGC 1 cut(s) 160
BstMAI GTCTC 3 cut(s) 231, 253, 391
BstMWI GCNNNNNNNGC 3 cut(s) 17, 83, 92
BstSCI CCNGG 1 cut(s) 186
BstSFI CTRYAG 1 cut(s) 18
BstSLI GKGCMC 1 cut(s) 115
BstUI CGCG 1 cut(s) 404
BstV1I GCAGC 5 cut(s) 4, 67, 70, 89, 142
BsuRI GGCC 1 cut(s) 47
BtgZI GCGATG 1 cut(s) 321
BtsCI GGATG 1 cut(s) 181
BtsIMutI CAGTG 2 cut(s) 115, 147
BtuMI TCGCGA 1 cut(s) 404
Cac8I GCNNGC 2 cut(s) 75, 115
CaiI CAGNNNCTG 1 cut(s) 83
CfoI GCGC 1 cut(s) 160
Cfr13I GGNCC 2 cut(s) 45, 339
CviAII CATG 2 cut(s) 161, 360
CviJI RGCY 6 cut(s) 11, 17, 47, 73, 80, 155
CviKI_1 RGCY 6 cut(s) 11, 17, 47, 73, 80, 155
DdeI CTNAG 1 cut(s) 124
Eam1104I CTCTTC 1 cut(s) 390
EarI CTCTTC 1 cut(s) 390
Eco130I CCWWGG 1 cut(s) 171
Eco31I GGTCTC 1 cut(s) 253
Eco47I GGWCC 1 cut(s) 339
Eco88I CYCGRG 1 cut(s) 347
EcoO109I RGGNCCY 1 cut(s) 339
EcoT14I CCWWGG 1 cut(s) 171
ErhI CCWWGG 1 cut(s) 171
FaeI CATG 2 cut(s) 164, 363
FaiI YATR 9 cut(s) 27, 29, 162, 300, 361, 368, 377, 390, 445
FalI AAGNNNNNCTT 2 cut(s) 164, 196
FatI CATG 2 cut(s) 160, 359
FblI GTMKAC 1 cut(s) 451
Fnu4HI GCNGC 5 cut(s) 18, 78, 81, 84, 156
FokI GGATG 1 cut(s) 188
Fsp4HI GCNGC 5 cut(s) 18, 78, 81, 84, 156
FspBI CTAG 1 cut(s) 336
FspI TGCGCA 1 cut(s) 159
GlaI GCGC 1 cut(s) 159
GluI GCNGC 5 cut(s) 18, 78, 81, 84, 156
HaeIII GGCC 1 cut(s) 47
HapII CCGG 1 cut(s) 187
HhaI GCGC 1 cut(s) 160
Hin1II CATG 2 cut(s) 164, 363
Hin6I GCGC 1 cut(s) 158
HinP1I GCGC 1 cut(s) 158
HincII GTYRAC 2 cut(s) 208, 452
HindII GTYRAC 2 cut(s) 208, 452
HinfI GANTC 3 cut(s) 165, 356, 399
HpaII CCGG 1 cut(s) 187
HphI GGTGA 2 cut(s) 160, 331
Hpy166II GTNNAC 3 cut(s) 113, 208, 452
Hpy188I TCNGA 1 cut(s) 194
Hpy188III TCNNGA 3 cut(s) 230, 347, 403
Hpy8I GTNNAC 3 cut(s) 113, 208, 452
Hpy99I CGWCG 2 cut(s) 453, 456
HpyCH4III ACNGT 3 cut(s) 63, 110, 456
HpyCH4V TGCA 3 cut(s) 20, 113, 184
HpyF10VI GCNNNNNNNGC 3 cut(s) 17, 83, 92
HpyF3I CTNAG 1 cut(s) 124
Hsp92II CATG 2 cut(s) 164, 363
HspAI GCGC 1 cut(s) 158
LpnPI CCDG 6 cut(s) 59, 105, 135, 200, 236, 316
Lsp1109I GCAGC 5 cut(s) 4, 67, 70, 89, 142
LweI GCATC 2 cut(s) 88, 166
MaeI CTAG 1 cut(s) 336
MaeIII GTNAC 2 cut(s) 145, 379
MboII GAAGA 1 cut(s) 377
MhlI GDGCHC 1 cut(s) 115
MluCI AATT 1 cut(s) 277
MlyI GAGTC 1 cut(s) 393
MmeI TCCRAC 1 cut(s) 45
MnlI CCTC 3 cut(s) 229, 309, 352
MseI TTAA 1 cut(s) 276
MspA1I CMGCKG 1 cut(s) 80
MspI CCGG 1 cut(s) 187
MspR9I CCNGG 1 cut(s) 188
MvnI CGCG 1 cut(s) 404
MwoI GCNNNNNNNGC 3 cut(s) 17, 83, 92
NciI CCSGG 1 cut(s) 188
NlaIII CATG 2 cut(s) 164, 363
NlaIV GGNNCC 1 cut(s) 46
NmuCI GTSAC 1 cut(s) 145
NruI TCGCGA 1 cut(s) 404
NsbI TGCGCA 1 cut(s) 159
PaeR7I CTCGAG 1 cut(s) 347
PfeI GAWTC 2 cut(s) 165, 356
PkrI GCNGC 5 cut(s) 19, 79, 82, 85, 157
PleI GAGTC 1 cut(s) 393
PpsI GAGTC 1 cut(s) 393
PpuMI RGGWCCY 1 cut(s) 339
Psp5II RGGWCCY 1 cut(s) 339
PspN4I GGNNCC 1 cut(s) 46
PspPI GGNCC 2 cut(s) 45, 339
PspPPI RGGWCCY 1 cut(s) 339
PstI CTGCAG 1 cut(s) 22
PstNI CAGNNNCTG 1 cut(s) 83
PvuII CAGCTG 1 cut(s) 80
RruI TCGCGA 1 cut(s) 404
SalI GTCGAC 1 cut(s) 450
SaqAI TTAA 1 cut(s) 276
SatI GCNGC 5 cut(s) 18, 78, 81, 84, 156
Sau96I GGNCC 2 cut(s) 45, 339
SchI GAGTC 1 cut(s) 393
ScrFI CCNGG 1 cut(s) 188
SduI GDGCHC 1 cut(s) 115
SetI ASST 5 cut(s) 35, 75, 82, 249, 344
SfaNI GCATC 2 cut(s) 88, 166
SfcI CTRYAG 1 cut(s) 18
Sfr274I CTCGAG 1 cut(s) 347
SgrDI CGTCGACG 1 cut(s) 450
SinI GGWCC 1 cut(s) 339
SlaI CTCGAG 1 cut(s) 347
SmlI CTYRAG 1 cut(s) 347
SmoI CTYRAG 1 cut(s) 347
Sse9I AATT 1 cut(s) 277
SspMI CTAG 1 cut(s) 336
StyD4I CCNGG 1 cut(s) 186
StyI CCWWGG 1 cut(s) 171
TaaI ACNGT 3 cut(s) 63, 110, 456
TaqI TCGA 3 cut(s) 330, 348, 451
TasI AATT 1 cut(s) 277
TfiI GAWTC 2 cut(s) 165, 356
Tru1I TTAA 1 cut(s) 276
Tru9I TTAA 1 cut(s) 276
TscAI CASTG 2 cut(s) 115, 154
TseFI GTSAC 1 cut(s) 145
TseI GCWGC 5 cut(s) 17, 77, 80, 83, 155
Tsp45I GTSAC 1 cut(s) 145
TspDTI ATGAA 3 cut(s) 339, 377, 383
TspRI CASTG 2 cut(s) 115, 154
VneI GTGCAC 1 cut(s) 111
VpaK11BI GGWCC 1 cut(s) 339
XhoI CTCGAG 1 cut(s) 347
XmiI GTMKAC 1 cut(s) 451
XspI CTAG 1 cut(s) 336
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.