RLG00000020343

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr4
OG0017116
Physical Location & Seq
Reverse (-)
63902801 .. 63903891
1091 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000020343

Sequence Viewer

Length: 609 bp
ATGGGCACAGATGAAAGCTCGAGGGTCAATCAAGGAGAACTATACTCCCCAGCTCACGCAATCAAAGTGATCAGCAACAAAGAAAACCATGAAAGCTCCGTGGACGTAGGTCAAGGAAAGTTGTACTCCCCGGCTCACGCAATCAAAGTAAGCAGCAAAGAAGAAAACCGAGATGAAAAATCGTCGTCGGAGCAACCAAGGACGACGTCGAATGCGGCGGTGGGAGTGGTGGAAGCAACAAAGGAAGTAGCAATGGACATGAAGAGGAACTTGGGGGCCGTCAAGGATAAGATACTGGAGAAGCTTGCCGCACACGCACCTGCTGTTCCGGCGGATGCTTTGGACAACGTGCGCCACTTCTTGGAGAGTGTTGTCAAGGACGTGACCGACCCGACCGGGGTGGCCCGTGGTGTCACCACGGGCACGGATGCGTTGCACCGTATCAAAACTCAACTTGGTGACATGCTGCCCAACCTCTCTCCAGACGCCACCAAGAAGATGGTGGATGAAGCGGAAAAGGAATTATTAGCTACTAATGAAGATGGTGGTAGATGCAAAGATGAAAGAAACCACAAGGAAGGAAATCAGAAACAATCATCGAGGCTTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

203

Amino Acids

21.85

Weight (kDa)

6.0

Isoelectric Point (pI)

35.95

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0017116)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G16730
fragaria_vesca FvH4_6g36360
malus_domestica MD04G1031600.v1.1
pyrus_communis pycom09g08720
rosa_laevigata RLG00000020343
rosa_multiflora Rmu_co8423113.1_g000001
rosa_roxburghii Rroxscaffold_2G00098830
rosa_rugosa Rorug02G0406100 Rorug02G0406100 Rorug02G0406100
rosa_samantha Rh2BG477800 Rh2DG486700
rosa_wichuraiana Rw2G037910

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 328
AatII GACGTC 1 cut(s) 209
Acc36I ACCTGC 1 cut(s) 328
AccB7I CCANNNNNTGG 1 cut(s) 361
AciI CCGC 5 cut(s) 215, 218, 309, 332, 512
AcyI GRCGYC 2 cut(s) 206, 486
AfaI GTAC 1 cut(s) 125
AfiI CCNNNNNNNGG 2 cut(s) 361, 397
AjiI CACGTC 1 cut(s) 382
AjuI GAANNNNNNNTTGG 2 cut(s) 254, 286
AluBI AGCT 5 cut(s) 18, 53, 96, 304, 530
AluI AGCT 5 cut(s) 18, 53, 96, 304, 530
Ama87I CYCGRG 1 cut(s) 19
AoxI GGCC 2 cut(s) 276, 402
ApeKI GCWGC 2 cut(s) 153, 466
AspLEI GCGC 1 cut(s) 354
AspS9I GGNCC 2 cut(s) 276, 403
AsuC2I CCSGG 2 cut(s) 131, 397
AsuHPI GGTGA 2 cut(s) 406, 470
AvaI CYCGRG 1 cut(s) 19
BaeGI GKGCMC 2 cut(s) 8, 425
BbvI GCAGC 2 cut(s) 165, 453
BccI CCATC 2 cut(s) 493, 536
BceAI ACGGC 1 cut(s) 263
BclI TGATCA 1 cut(s) 69
BcnI CCSGG 2 cut(s) 131, 397
BfuAI ACCTGC 1 cut(s) 328
BisI GCNGC 4 cut(s) 154, 216, 309, 467
BlsI GCNGC 4 cut(s) 155, 217, 310, 468
Bme1390I CCNGG 2 cut(s) 131, 397
BmeT110I CYCGRG 1 cut(s) 19
BmgBI CACGTC 1 cut(s) 382
BmgT120I GGNCC 2 cut(s) 276, 403
BmiI GGNNCC 1 cut(s) 277
BmrFI CCNGG 2 cut(s) 131, 397
BmsI GCATC 3 cut(s) 325, 418, 542
BoxI GACNNNNGTC 1 cut(s) 108
BpmI CTGGAG 2 cut(s) 317, 465
BpuMI CCSGG 2 cut(s) 131, 397
BsaHI GRCGYC 2 cut(s) 206, 486
BsaJI CCNNGG 6 cut(s) 99, 129, 197, 396, 406, 417
Bsc4I CCNNNNNNNGG 2 cut(s) 361, 397
Bse1I ACTGG 1 cut(s) 300
Bse3DI GCAATG 1 cut(s) 258
BseDI CCNNGG 6 cut(s) 99, 129, 197, 396, 406, 417
BseGI GGATG 3 cut(s) 340, 433, 511
BseLI CCNNNNNNNGG 2 cut(s) 361, 397
BseMI GCAATG 1 cut(s) 258
BseNI ACTGG 1 cut(s) 300
BseSI GKGCMC 2 cut(s) 8, 425
BseXI GCAGC 2 cut(s) 165, 453
BseYI CCCAGC 1 cut(s) 49
Bsh1285I CGRYCG 1 cut(s) 396
BshFI GGCC 2 cut(s) 278, 404
BsiEI CGRYCG 1 cut(s) 396
BsiHKCI CYCGRG 1 cut(s) 19
BsiSI CCGG 3 cut(s) 131, 329, 396
BslI CCNNNNNNNGG 2 cut(s) 361, 397
BsmI GAATGC 1 cut(s) 217
BsnI GGCC 2 cut(s) 278, 404
BsoBI CYCGRG 1 cut(s) 19
Bsp1286I GDGCHC 2 cut(s) 8, 425
Bsp143I GATC 1 cut(s) 69
BspACI CCGC 5 cut(s) 215, 218, 309, 332, 512
BspANI GGCC 2 cut(s) 278, 404
BspLI GGNNCC 1 cut(s) 277
BspMI ACCTGC 1 cut(s) 328
BsrDI GCAATG 1 cut(s) 258
BsrI ACTGG 1 cut(s) 300
BssECI CCNNGG 6 cut(s) 99, 129, 197, 396, 406, 417
BssMI GATC 1 cut(s) 69
BssNI GRCGYC 2 cut(s) 206, 486
BssT1I CCWWGG 1 cut(s) 197
Bst4CI ACNGT 1 cut(s) 440
Bst6I CTCTTC 1 cut(s) 257
BstACI GRCGYC 2 cut(s) 206, 486
BstC8I GCNNGC 1 cut(s) 306
BstDSI CCRYGG 3 cut(s) 99, 406, 417
BstF5I GGATG 3 cut(s) 340, 433, 511
BstHHI GCGC 1 cut(s) 354
BstKTI GATC 1 cut(s) 72
BstMBI GATC 1 cut(s) 69
BstMCI CGRYCG 1 cut(s) 396
BstMWI GCNNNNNNNGC 2 cut(s) 314, 329
BstNSI RCATGY 1 cut(s) 466
BstPAI GACNNNNGTC 1 cut(s) 108
BstSCI CCNGG 2 cut(s) 129, 395
BstSLI GKGCMC 2 cut(s) 8, 425
BstV1I GCAGC 2 cut(s) 165, 453
BstXI CCANNNNNNTGG 1 cut(s) 499
BsuRI GGCC 2 cut(s) 278, 404
BtgI CCRYGG 3 cut(s) 99, 406, 417
BtrI CACGTC 1 cut(s) 382
BtsCI GGATG 3 cut(s) 340, 433, 511
BveI ACCTGC 1 cut(s) 328
Cac8I GCNNGC 1 cut(s) 306
CfoI GCGC 1 cut(s) 354
Cfr13I GGNCC 2 cut(s) 276, 403
CseI GACGC 1 cut(s) 494
Csp6I GTAC 1 cut(s) 124
CviAII CATG 3 cut(s) 89, 259, 463
CviJI RGCY 9 cut(s) 18, 53, 96, 134, 278, 304, 404, 530, 604
CviKI_1 RGCY 9 cut(s) 18, 53, 96, 134, 278, 304, 404, 530, 604
CviQI GTAC 1 cut(s) 124
DpnI GATC 1 cut(s) 71
DpnII GATC 1 cut(s) 69
Eam1104I CTCTTC 1 cut(s) 257
EarI CTCTTC 1 cut(s) 257
EciI GGCGGA 1 cut(s) 347
Eco130I CCWWGG 1 cut(s) 197
Eco88I CYCGRG 1 cut(s) 19
EcoT14I CCWWGG 1 cut(s) 197
ErhI CCWWGG 1 cut(s) 197
FaeI CATG 3 cut(s) 92, 262, 466
FaiI YATR 4 cut(s) 43, 90, 260, 464
FalI AAGNNNNNCTT 2 cut(s) 254, 286
FatI CATG 3 cut(s) 88, 258, 462
FbaI TGATCA 1 cut(s) 69
Fnu4HI GCNGC 4 cut(s) 154, 216, 309, 467
FokI GGATG 3 cut(s) 347, 440, 518
Fsp4HI GCNGC 4 cut(s) 154, 216, 309, 467
GlaI GCGC 1 cut(s) 353
GluI GCNGC 4 cut(s) 154, 216, 309, 467
GsaI CCCAGC 1 cut(s) 53
GsuI CTGGAG 2 cut(s) 317, 465
HaeIII GGCC 2 cut(s) 278, 404
HapII CCGG 3 cut(s) 131, 329, 396
HgaI GACGC 1 cut(s) 494
HhaI GCGC 1 cut(s) 354
Hin1I GRCGYC 2 cut(s) 206, 486
Hin1II CATG 3 cut(s) 92, 262, 466
Hin6I GCGC 1 cut(s) 352
HinP1I GCGC 1 cut(s) 352
HindIII AAGCTT 1 cut(s) 302
HpaII CCGG 3 cut(s) 131, 329, 396
HphI GGTGA 2 cut(s) 406, 470
Hpy166II GTNNAC 1 cut(s) 103
Hpy188I TCNGA 2 cut(s) 190, 588
Hpy188III TCNNGA 1 cut(s) 482
Hpy8I GTNNAC 1 cut(s) 103
Hpy99I CGWCG 4 cut(s) 187, 190, 208, 211
HpyAV CCTTC 1 cut(s) 572
HpyCH4III ACNGT 1 cut(s) 440
HpyCH4IV ACGT 4 cut(s) 105, 206, 348, 381
HpyCH4V TGCA 2 cut(s) 436, 555
HpyF10VI GCNNNNNNNGC 2 cut(s) 314, 329
HpySE526I ACGT 4 cut(s) 105, 206, 348, 381
Hsp92I GRCGYC 2 cut(s) 206, 486
Hsp92II CATG 3 cut(s) 92, 262, 466
HspAI GCGC 1 cut(s) 352
Ksp22I TGATCA 1 cut(s) 69
Kzo9I GATC 1 cut(s) 69
LmnI GCTCC 2 cut(s) 101, 190
LpnPI CCDG 7 cut(s) 63, 144, 281, 333, 342, 409, 495
Lsp1109I GCAGC 2 cut(s) 165, 453
LweI GCATC 3 cut(s) 325, 418, 542
MaeII ACGT 4 cut(s) 105, 206, 348, 381
MaeIII GTNAC 3 cut(s) 382, 412, 458
MalI GATC 1 cut(s) 71
MboI GATC 1 cut(s) 69
MboII GAAGA 4 cut(s) 173, 274, 508, 551
MhlI GDGCHC 2 cut(s) 8, 425
MluCI AATT 1 cut(s) 521
MmeI TCCRAC 1 cut(s) 168
MnlI CCTC 4 cut(s) 15, 258, 485, 594
MspI CCGG 3 cut(s) 131, 329, 396
MspR9I CCNGG 2 cut(s) 131, 397
Mva1269I GAATGC 1 cut(s) 217
MwoI GCNNNNNNNGC 2 cut(s) 314, 329
NciI CCSGG 2 cut(s) 131, 397
NdeII GATC 1 cut(s) 69
NlaIII CATG 3 cut(s) 92, 262, 466
NlaIV GGNNCC 1 cut(s) 277
NmuCI GTSAC 3 cut(s) 382, 412, 458
NspI RCATGY 1 cut(s) 466
PaeR7I CTCGAG 1 cut(s) 19
PaqCI CACCTGC 1 cut(s) 328
PctI GAATGC 1 cut(s) 217
PflFI GACNNNGTC 1 cut(s) 205
PflMI CCANNNNNTGG 1 cut(s) 361
PkrI GCNGC 4 cut(s) 155, 217, 310, 468
PshAI GACNNNNGTC 1 cut(s) 108
PspFI CCCAGC 1 cut(s) 49
PspN4I GGNNCC 1 cut(s) 277
PspPI GGNCC 2 cut(s) 276, 403
PspXI VCTCGAGB 1 cut(s) 19
PsyI GACNNNGTC 1 cut(s) 205
RsaI GTAC 1 cut(s) 125
RsaNI GTAC 1 cut(s) 124
SatI GCNGC 4 cut(s) 154, 216, 309, 467
Sau3AI GATC 1 cut(s) 69
Sau96I GGNCC 2 cut(s) 276, 403
ScrFI CCNGG 2 cut(s) 131, 397
SduI GDGCHC 2 cut(s) 8, 425
SfaNI GCATC 3 cut(s) 325, 418, 542
Sfr274I CTCGAG 1 cut(s) 19
SlaI CTCGAG 1 cut(s) 19
SmlI CTYRAG 1 cut(s) 19
SmoI CTYRAG 1 cut(s) 19
Sse9I AATT 1 cut(s) 521
SsiI CCGC 5 cut(s) 215, 218, 309, 332, 512
StyD4I CCNGG 2 cut(s) 129, 395
StyI CCWWGG 1 cut(s) 197
TaaI ACNGT 1 cut(s) 440
TaiI ACGT 4 cut(s) 108, 209, 351, 384
TaqI TCGA 3 cut(s) 20, 209, 599
TaqII GACCGA 1 cut(s) 401
TasI AATT 1 cut(s) 521
TatI WGTACW 1 cut(s) 123
TauI GCSGC 2 cut(s) 218, 311
TseFI GTSAC 3 cut(s) 382, 412, 458
TseI GCWGC 2 cut(s) 153, 466
Tsp45I GTSAC 3 cut(s) 382, 412, 458
TspDTI ATGAA 7 cut(s) 27, 105, 189, 275, 522, 552, 576
TspGWI ACGGA 2 cut(s) 88, 440
Tth111I GACNNNGTC 1 cut(s) 205
Van91I CCANNNNNTGG 1 cut(s) 361
XceI RCATGY 1 cut(s) 466
XcmI CCANNNNNNNNNTGG 2 cut(s) 496, 499
XhoI CTCGAG 1 cut(s) 19
ZraI GACGTC 1 cut(s) 207
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.