MD04G1035300.v1.1

Toll - interleukin 1 - resistance

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr04
OG0017724
Physical Location & Seq
Forward (+)
3971517 .. 3973035
1519 bp
Loading structure...
UTR
Exon/CDS
Intron
MD04G1035300.v1.1.491

Sequence Viewer

Length: 393 bp
ATGGAAAATTTGAATTCCTTAAAATTAAGTGGAACAGCAGTTCAAGAGCTACACTCATCAATCGAGTTTCTCCCTGCTCTCCAAGTACTTGAACTACATGATTGCAAAAGGCTTTCAAGTATCCCAAAGAGCATTTGTAAGTTGAAGTATCTATTGGAGCTCGATATCGATGGGTGCTCCGAACTTAAACATTCCCCAGAGATCTTTAAGCTAATGAAACATTTGGAGCCAACGAGACATATTTTTGAACGCTTACAGGCTGCCCTCATTGTTCAGAAAGCAGCCTTACAATTTATTCAGTCCAAAAAGACTACAAGAAAAAGCCCTTATTGCGACAGGTTTTTTGCGTCAGATAGCACGGCCCTCACAAATAACACAAAATATGCGCGCTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

131

Amino Acids

14.86

Weight (kDa)

9.1

Isoelectric Point (pI)

46.16

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
LRR_Zer-1 PF25013 2 - 61 1e-05 Zer-1-like, Leucine-rich repeats
LRR_14 PF23598 4 - 77 1.9e-08 Leucine-rich repeat region
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccII CGCG 1 cut(s) 388
AcsI RAATTY 2 cut(s) 7, 13
AfaI GTAC 1 cut(s) 87
AgsI TTSAA 6 cut(s) 13, 44, 92, 117, 145, 248
AjuI GAANNNNNNNTTGG 2 cut(s) 137, 169
AloI GAACNNNNNNTCC 2 cut(s) 24, 56
AluBI AGCT 3 cut(s) 49, 160, 211
AluI AGCT 3 cut(s) 49, 160, 211
Alw21I GWGCWC 2 cut(s) 162, 179
Alw26I GTCTC 1 cut(s) 229
AoxI GGCC 1 cut(s) 360
ApeKI GCWGC 2 cut(s) 260, 281
ApoI RAATTY 2 cut(s) 7, 13
ArsI GACNNNNNNTTYG 2 cut(s) 326, 358
AspLEI GCGC 2 cut(s) 388, 390
AspS9I GGNCC 1 cut(s) 361
BanII GRGCYC 1 cut(s) 162
Bbv12I GWGCWC 2 cut(s) 162, 179
BbvI GCAGC 2 cut(s) 247, 293
BccI CCATC 1 cut(s) 164
BceAI ACGGC 1 cut(s) 375
BciVI GTATCC 1 cut(s) 131
BcoDI GTCTC 1 cut(s) 229
BfaI CTAG 1 cut(s) 391
BfuI GTATCC 1 cut(s) 131
BglII AGATCT 1 cut(s) 201
BisI GCNGC 2 cut(s) 261, 282
BlsI GCNGC 2 cut(s) 262, 283
BmcAI AGTACT 1 cut(s) 87
BmgT120I GGNCC 1 cut(s) 361
BmiI GGNNCC 1 cut(s) 228
BplI GAGNNNNNCTC 2 cut(s) 38, 70
Bsa29I ATCGAT 1 cut(s) 168
BseCI ATCGAT 1 cut(s) 168
BsePI GCGCGC 1 cut(s) 386
BseXI GCAGC 2 cut(s) 247, 293
Bsh1236I CGCG 1 cut(s) 388
BshFI GGCC 1 cut(s) 362
BshVI ATCGAT 1 cut(s) 168
BsiHKAI GWGCWC 2 cut(s) 162, 179
BsmAI GTCTC 1 cut(s) 229
BsnI GGCC 1 cut(s) 362
Bsp1286I GDGCHC 2 cut(s) 162, 179
Bsp143I GATC 1 cut(s) 201
BspANI GGCC 1 cut(s) 362
BspDI ATCGAT 1 cut(s) 168
BspFNI CGCG 1 cut(s) 388
BspLI GGNNCC 1 cut(s) 228
BssHII GCGCGC 1 cut(s) 386
BssMI GATC 1 cut(s) 201
BstC8I GCNNGC 1 cut(s) 388
BstFNI CGCG 1 cut(s) 388
BstHHI GCGC 2 cut(s) 388, 390
BstKTI GATC 1 cut(s) 204
BstMAI GTCTC 1 cut(s) 229
BstMBI GATC 1 cut(s) 201
BstMWI GCNNNNNNNGC 1 cut(s) 330
BstUI CGCG 1 cut(s) 388
BstV1I GCAGC 2 cut(s) 247, 293
BstX2I RGATCY 1 cut(s) 201
BstYI RGATCY 1 cut(s) 201
Bsu15I ATCGAT 1 cut(s) 168
BsuI GTATCC 1 cut(s) 131
BsuRI GGCC 1 cut(s) 362
BsuTUI ATCGAT 1 cut(s) 168
Cac8I GCNNGC 1 cut(s) 388
CfoI GCGC 2 cut(s) 388, 390
Cfr13I GGNCC 1 cut(s) 361
ClaI ATCGAT 1 cut(s) 168
CseI GACGC 1 cut(s) 336
Csp6I GTAC 1 cut(s) 86
CviAII CATG 1 cut(s) 98
CviJI RGCY 9 cut(s) 49, 112, 160, 211, 229, 260, 284, 324, 362
CviKI_1 RGCY 9 cut(s) 49, 112, 160, 211, 229, 260, 284, 324, 362
CviQI GTAC 1 cut(s) 86
DpnI GATC 1 cut(s) 203
DpnII GATC 1 cut(s) 201
Ecl136II GAGCTC 1 cut(s) 160
Eco24I GRGCYC 1 cut(s) 162
Eco32I GATATC 1 cut(s) 166
Eco53kI GAGCTC 1 cut(s) 160
EcoICRI GAGCTC 1 cut(s) 160
EcoRI GAATTC 1 cut(s) 13
EcoRV GATATC 1 cut(s) 166
EcoT38I GRGCYC 1 cut(s) 162
FaeI CATG 1 cut(s) 101
FaiI YATR 3 cut(s) 99, 240, 384
FatI CATG 1 cut(s) 97
Fnu4HI GCNGC 2 cut(s) 261, 282
FriOI GRGCYC 1 cut(s) 162
Fsp4HI GCNGC 2 cut(s) 261, 282
FspBI CTAG 1 cut(s) 391
GlaI GCGC 2 cut(s) 387, 389
GluI GCNGC 2 cut(s) 261, 282
HaeIII GGCC 1 cut(s) 362
HgaI GACGC 1 cut(s) 336
HhaI GCGC 2 cut(s) 388, 390
Hin1II CATG 1 cut(s) 101
Hin6I GCGC 2 cut(s) 386, 388
HinP1I GCGC 2 cut(s) 386, 388
Hpy188I TCNGA 3 cut(s) 181, 276, 352
Hpy188III TCNNGA 1 cut(s) 44
HpyCH4V TGCA 1 cut(s) 105
HpyF10VI GCNNNNNNNGC 1 cut(s) 330
Hsp92II CATG 1 cut(s) 101
HspAI GCGC 2 cut(s) 386, 388
Kzo9I GATC 1 cut(s) 201
LmnI GCTCC 3 cut(s) 157, 182, 226
LpnPI CCDG 4 cut(s) 87, 210, 242, 322
Lsp1109I GCAGC 2 cut(s) 247, 293
MaeI CTAG 1 cut(s) 391
MalI GATC 1 cut(s) 203
MboI GATC 1 cut(s) 201
MflI RGATCY 1 cut(s) 201
MhlI GDGCHC 2 cut(s) 162, 179
MluCI AATT 4 cut(s) 7, 13, 23, 290
MnlI CCTC 2 cut(s) 275, 374
MseI TTAA 4 cut(s) 20, 26, 186, 207
MvnI CGCG 1 cut(s) 388
MwoI GCNNNNNNNGC 1 cut(s) 330
NdeII GATC 1 cut(s) 201
NlaIII CATG 1 cut(s) 101
NlaIV GGNNCC 1 cut(s) 228
PauI GCGCGC 1 cut(s) 386
PkrI GCNGC 2 cut(s) 262, 283
Psp124BI GAGCTC 1 cut(s) 162
PspN4I GGNNCC 1 cut(s) 228
PspPI GGNCC 1 cut(s) 361
PsuI RGATCY 1 cut(s) 201
PteI GCGCGC 1 cut(s) 386
RsaI GTAC 1 cut(s) 87
RsaNI GTAC 1 cut(s) 86
SacI GAGCTC 1 cut(s) 162
SaqAI TTAA 4 cut(s) 20, 26, 186, 207
SatI GCNGC 2 cut(s) 261, 282
Sau3AI GATC 1 cut(s) 201
Sau96I GGNCC 1 cut(s) 361
ScaI AGTACT 1 cut(s) 87
SduI GDGCHC 2 cut(s) 162, 179
SetI ASST 4 cut(s) 51, 162, 213, 341
Sse9I AATT 4 cut(s) 7, 13, 23, 290
SspMI CTAG 1 cut(s) 391
SstI GAGCTC 1 cut(s) 162
TaqI TCGA 3 cut(s) 63, 162, 168
TasI AATT 4 cut(s) 7, 13, 23, 290
TatI WGTACW 1 cut(s) 85
Tru1I TTAA 4 cut(s) 20, 26, 186, 207
Tru9I TTAA 4 cut(s) 20, 26, 186, 207
TseI GCWGC 2 cut(s) 260, 281
TspDTI ATGAA 1 cut(s) 230
XapI RAATTY 2 cut(s) 7, 13
XspI CTAG 1 cut(s) 391
ZrmI AGTACT 1 cut(s) 87
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.