MD08G1230100.v1.1

RESISTANCE protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr08
OG0017724
Physical Location & Seq
Reverse (-)
29570858 .. 29574977
4120 bp
Loading structure...
UTR
Exon/CDS
Intron
MD08G1230100.v1.1.491

Sequence Viewer

Length: 1197 bp
ATGAGATCTCTCATAACCCTTGATCTTTCTGGCTGCTCGAGTCTTGAGATGTTTCCAGAGATTTTAGAAGTTATGGAGGAACTACCAGAGCTTAATTTATCCGAGTCAAAAATTAAAGAATTGCCCTCATCAATTAATAATCTCACGGGGTTGAGATATTTGAAACTAACAAATTGCAAGGAACTCAAGAACCTTCCAAGCATTCGTATGAAATCTCTCAAAATCCTTGATCTTTCTGGTTGCTCGAGTCTTGAGATGTTTCCAGAGATTTTAGAAGTTATGGAAGAACTACCAGAGCTTAATTTATCCGGGTTAAAAATTGAAGAATTGCCCTCATCTATTAATAATCTCACGGGGTTGAGATATTTGAAACTAACAAATTGCAAGGAACTCAAGAGCCTTCCAAGCATTCGTATGAAATCTCTCGTAACCCTTGATCTTTCTGGCTGCTCGAGTCTTGAAATGTTTCCTGAGATTTTAGAAGTTATGGAGGAACTACAAAAGCTTAATTTATTCGGGTCAAAAATTAAAGAATTGCCCTCATCAATTAATAATCTCACAGGGTTGAGATATTTGGACCTGAAAGATTGCAAGGAACTCAAGAGTCTTCCAAGCTTCATTCATATGAGATCTCTCGCAGCCCTTAATCTTTATGGCTGCTCGAGTCTTGCGATGTTTCCAGAGATTTTAGAAGTTATGGATCAACTAATAGGGCTTGATTTATCCGGATCAAAAATTAAAGAATTGCCCTCGTCAATTAATTGTCTCACGGGATTGAGAATTTTGATCCTAAAAGATTGCAAAGAACTCAAGACTCTCCCAAGTAGCATTTGTCAACTTCAATCCCTATATTATCTCTCTCTTTCTGGTTGTACAAAATTTGAGGTGTTTCCAAACATTGAAGAAAATATGGAAGAATTAAGAGCGCTTCATTTGGATGGAACATCTATCAAAGAGCTTCCCCCCTCGATTGAACGGCTTCAGCAGCTTGTGTTATTAAATCTGGGAAACTGCGAAAGCCTTGTGCATCTTCCCGACACTTTCTGTAATTTGGCGCGCCCTTTAGCTGCCAATCTCAGTGGGTGCACAAATCTTTCTCAATTTCCCGTCGACTATGAGGATTTAGCACGCTTGTGGGATTATCAAGTAGATATTGGAAGGGGCGTGCTGAATTTGACACGTATATTACTCTCCTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
LRR_1 LRR_3 LRR_5 LRR_8 NB-ARC TIR

Protein Analysis

399

Amino Acids

44.62

Weight (kDa)

4.97

Isoelectric Point (pI)

50.28

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
LRR_13 PF23286 3 - 67 2.1e-09 Disease resistance protein RPS4B-like, leucine-rich repeats
LRR_14 PF23598 69 - 171 2.7e-07 Leucine-rich repeat region
LRR_13 PF23286 71 - 137 5.2e-09 Disease resistance protein RPS4B-like, leucine-rich repeats
LRR_13 PF23286 140 - 223 3e-13 Disease resistance protein RPS4B-like, leucine-rich repeats
LRR_14 PF23598 144 - 236 4.2e-07 Leucine-rich repeat region
LRR_R13L1-DRL21 PF25019 159 - 220 3.5e-06 R13L1/DRL21 LRRs
LRR_13 PF23286 210 - 292 9.1e-10 Disease resistance protein RPS4B-like, leucine-rich repeats
LRR_14 PF23598 239 - 310 8.2e-10 Leucine-rich repeat region
LRR_13 PF23286 280 - 366 4.1e-08 Disease resistance protein RPS4B-like, leucine-rich repeats
LRR_14 PF23598 303 - 353 1.4e-06 Leucine-rich repeat region
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccI GTMKAC 1 cut(s) 1110
AccII CGCG 1 cut(s) 1057
AccIII TCCGGA 1 cut(s) 725
AclWI GGATC 3 cut(s) 708, 736, 781
AcsI RAATTY 3 cut(s) 780, 878, 1171
AcuI CTGAAG 1 cut(s) 965
AfaI GTAC 1 cut(s) 874
AfeI AGCGCT 1 cut(s) 927
AflIII ACRYGT 1 cut(s) 1178
AgsI TTSAA 7 cut(s) 163, 323, 370, 461, 842, 902, 974
AleI CACNNNNGTG 1 cut(s) 1132
AluBI AGCT 7 cut(s) 91, 298, 505, 615, 958, 988, 1067
AluI AGCT 7 cut(s) 91, 298, 505, 615, 958, 988, 1067
Alw21I GWGCWC 1 cut(s) 1088
Alw26I GTCTC 1 cut(s) 770
Alw44I GTGCAC 1 cut(s) 1084
AlwI GGATC 3 cut(s) 708, 736, 781
Ama87I CYCGRG 4 cut(s) 37, 244, 451, 661
Aor13HI TCCGGA 1 cut(s) 725
Aor51HI AGCGCT 1 cut(s) 927
ApaLI GTGCAC 1 cut(s) 1084
ApeKI GCWGC 6 cut(s) 33, 447, 638, 657, 985, 1067
ApoI RAATTY 3 cut(s) 780, 878, 1171
AscI GGCGCGCC 1 cut(s) 1055
AseI ATTAAT 4 cut(s) 135, 342, 549, 759
Asp700I GAANNNNTTC 2 cut(s) 465, 978
AspLEI GCGC 3 cut(s) 928, 1057, 1059
AspS9I GGNCC 1 cut(s) 577
AsuC2I CCSGG 1 cut(s) 310
AvaI CYCGRG 4 cut(s) 37, 244, 451, 661
AvaII GGWCC 1 cut(s) 577
BaeGI GKGCMC 1 cut(s) 1088
BbsI GAAGAC 1 cut(s) 599
Bbv12I GWGCWC 1 cut(s) 1088
BbvI GCAGC 6 cut(s) 20, 434, 644, 650, 997, 1054
BccI CCATC 1 cut(s) 932
BceAI ACGGC 1 cut(s) 992
BcnI CCSGG 1 cut(s) 310
BcoDI GTCTC 1 cut(s) 770
BfoI RGCGCY 1 cut(s) 929
BglII AGATCT 2 cut(s) 5, 629
BisI GCNGC 6 cut(s) 34, 448, 639, 658, 986, 1068
BlsI GCNGC 6 cut(s) 35, 449, 640, 659, 987, 1069
Bme1390I CCNGG 1 cut(s) 310
Bme18I GGWCC 1 cut(s) 577
BmeT110I CYCGRG 4 cut(s) 37, 244, 451, 661
BmgT120I GGNCC 1 cut(s) 577
BmrFI CCNGG 1 cut(s) 310
BmsI GCATC 1 cut(s) 1036
BpiI GAAGAC 1 cut(s) 599
BpuEI CTTGAG 6 cut(s) 65, 170, 272, 377, 584, 794
BpuMI CCSGG 1 cut(s) 310
BsaAI YACGTR 1 cut(s) 1181
BsaWI WCCGGW 1 cut(s) 725
BseAI TCCGGA 1 cut(s) 725
BseGI GGATG 1 cut(s) 943
BseMII CTCAG 2 cut(s) 462, 1090
BsePI GCGCGC 1 cut(s) 1055
BseSI GKGCMC 1 cut(s) 1088
BseXI GCAGC 6 cut(s) 20, 434, 644, 650, 997, 1054
Bsh1236I CGCG 1 cut(s) 1057
BsiHKAI GWGCWC 1 cut(s) 1088
BsiHKCI CYCGRG 4 cut(s) 37, 244, 451, 661
BsiSI CCGG 2 cut(s) 309, 726
BsmAI GTCTC 1 cut(s) 770
BsmI GAATGC 2 cut(s) 201, 408
BsoBI CYCGRG 4 cut(s) 37, 244, 451, 661
Bsp1286I GDGCHC 1 cut(s) 1088
Bsp13I TCCGGA 1 cut(s) 725
Bsp1407I TGTACA 1 cut(s) 872
Bsp143I GATC 8 cut(s) 5, 22, 229, 436, 629, 700, 728, 786
BspCNI CTCAG 2 cut(s) 463, 1089
BspEI TCCGGA 1 cut(s) 725
BspFNI CGCG 1 cut(s) 1057
BspPI GGATC 3 cut(s) 708, 736, 781
BsrGI TGTACA 1 cut(s) 872
BssHII GCGCGC 1 cut(s) 1055
BssMI GATC 8 cut(s) 5, 22, 229, 436, 629, 700, 728, 786
BstAUI TGTACA 1 cut(s) 872
BstBAI YACGTR 1 cut(s) 1181
BstC8I GCNNGC 3 cut(s) 1057, 1129, 1166
BstDEI CTNAG 2 cut(s) 471, 1076
BstF5I GGATG 1 cut(s) 943
BstFNI CGCG 1 cut(s) 1057
BstH2I RGCGCY 1 cut(s) 929
BstHHI GCGC 3 cut(s) 928, 1057, 1059
BstKTI GATC 8 cut(s) 8, 25, 232, 439, 632, 703, 731, 789
BstMAI GTCTC 1 cut(s) 770
BstMBI GATC 8 cut(s) 5, 22, 229, 436, 629, 700, 728, 786
BstMWI GCNNNNNNNGC 2 cut(s) 405, 985
BstSCI CCNGG 1 cut(s) 308
BstSLI GKGCMC 1 cut(s) 1088
BstUI CGCG 1 cut(s) 1057
BstV1I GCAGC 6 cut(s) 20, 434, 644, 650, 997, 1054
BstV2I GAAGAC 1 cut(s) 599
BstX2I RGATCY 2 cut(s) 5, 629
BstYI RGATCY 2 cut(s) 5, 629
BtgZI GCGATG 1 cut(s) 686
BtsCI GGATG 1 cut(s) 943
BtsIMutI CAGTG 1 cut(s) 1084
Cac8I GCNNGC 3 cut(s) 1057, 1129, 1166
CfoI GCGC 3 cut(s) 928, 1057, 1059
Cfr13I GGNCC 1 cut(s) 577
Csp6I GTAC 1 cut(s) 873
CspCI CAANNNNNGTGG 2 cut(s) 1060, 1095
CviQI GTAC 1 cut(s) 873
DdeI CTNAG 2 cut(s) 471, 1076
DpnI GATC 8 cut(s) 7, 24, 231, 438, 631, 702, 730, 788
DpnII GATC 8 cut(s) 5, 22, 229, 436, 629, 700, 728, 786
Eco47I GGWCC 1 cut(s) 577
Eco47III AGCGCT 1 cut(s) 927
Eco57I CTGAAG 1 cut(s) 965
Eco88I CYCGRG 4 cut(s) 37, 244, 451, 661
FauNDI CATATG 1 cut(s) 624
FblI GTMKAC 1 cut(s) 1110
Fnu4HI GCNGC 6 cut(s) 34, 448, 639, 658, 986, 1068
FokI GGATG 1 cut(s) 950
Fsp4HI GCNGC 6 cut(s) 34, 448, 639, 658, 986, 1068
GlaI GCGC 3 cut(s) 927, 1056, 1058
GluI GCNGC 6 cut(s) 34, 448, 639, 658, 986, 1068
HaeII RGCGCY 1 cut(s) 929
HapII CCGG 2 cut(s) 309, 726
HhaI GCGC 3 cut(s) 928, 1057, 1059
Hin6I GCGC 3 cut(s) 926, 1055, 1057
HinP1I GCGC 3 cut(s) 926, 1055, 1057
HincII GTYRAC 2 cut(s) 836, 1111
HindII GTYRAC 2 cut(s) 836, 1111
HindIII AAGCTT 2 cut(s) 503, 613
HinfI GANTC 7 cut(s) 40, 104, 247, 454, 604, 664, 814
HpaII CCGG 2 cut(s) 309, 726
Hpy166II GTNNAC 3 cut(s) 836, 1086, 1111
Hpy188I TCNGA 1 cut(s) 103
Hpy8I GTNNAC 3 cut(s) 836, 1086, 1111
Hpy99I CGWCG 1 cut(s) 1112
HpyAV CCTTC 3 cut(s) 203, 410, 1152
HpyCH4IV ACGT 1 cut(s) 1180
HpyCH4V TGCA 6 cut(s) 177, 384, 591, 801, 1027, 1086
HpyF10VI GCNNNNNNNGC 2 cut(s) 405, 985
HpyF3I CTNAG 2 cut(s) 471, 1076
HpySE526I ACGT 1 cut(s) 1180
HspAI GCGC 3 cut(s) 926, 1055, 1057
Kpn2I TCCGGA 1 cut(s) 725
Kzo9I GATC 8 cut(s) 5, 22, 229, 436, 629, 700, 728, 786
Lsp1109I GCAGC 6 cut(s) 20, 434, 644, 650, 997, 1054
LweI GCATC 1 cut(s) 1036
MaeII ACGT 1 cut(s) 1180
MaeIII GTNAC 1 cut(s) 427
MalI GATC 8 cut(s) 7, 24, 231, 438, 631, 702, 730, 788
MboI GATC 8 cut(s) 5, 22, 229, 436, 629, 700, 728, 786
MboII GAAGA 6 cut(s) 296, 335, 599, 914, 926, 1022
MflI RGATCY 2 cut(s) 5, 629
MhlI GDGCHC 1 cut(s) 1088
MlyI GAGTC 7 cut(s) 49, 113, 256, 463, 613, 673, 808
MnlI CCTC 9 cut(s) 70, 136, 343, 484, 550, 760, 877, 976, 1111
MroI TCCGGA 1 cut(s) 725
MroXI GAANNNNTTC 2 cut(s) 465, 978
MslI CAYNNNNRTG 5 cut(s) 206, 413, 623, 936, 1132
MspI CCGG 2 cut(s) 309, 726
MspR9I CCNGG 1 cut(s) 310
Mva1269I GAATGC 2 cut(s) 201, 408
MvnI CGCG 1 cut(s) 1057
MwoI GCNNNNNNNGC 2 cut(s) 405, 985
NciI CCSGG 1 cut(s) 310
NdeI CATATG 1 cut(s) 624
NdeII GATC 8 cut(s) 5, 22, 229, 436, 629, 700, 728, 786
OliI CACNNNNGTG 1 cut(s) 1132
PaeR7I CTCGAG 4 cut(s) 37, 244, 451, 661
PalAI GGCGCGCC 1 cut(s) 1055
PauI GCGCGC 1 cut(s) 1055
PcsI WCGNNNNNNNCGW 1 cut(s) 668
PctI GAATGC 2 cut(s) 201, 408
PdmI GAANNNNTTC 2 cut(s) 465, 978
PkrI GCNGC 6 cut(s) 35, 449, 640, 659, 987, 1069
PleI GAGTC 7 cut(s) 48, 112, 255, 462, 612, 672, 808
PpsI GAGTC 7 cut(s) 48, 112, 255, 462, 612, 672, 808
Ppu21I YACGTR 1 cut(s) 1181
PshBI ATTAAT 4 cut(s) 135, 342, 549, 759
PspPI GGNCC 1 cut(s) 577
PspXI VCTCGAGB 4 cut(s) 37, 244, 451, 661
PsuI RGATCY 2 cut(s) 5, 629
PteI GCGCGC 1 cut(s) 1055
RsaI GTAC 1 cut(s) 874
RsaNI GTAC 1 cut(s) 873
RseI CAYNNNNRTG 5 cut(s) 206, 413, 623, 936, 1132
SalI GTCGAC 1 cut(s) 1109
SatI GCNGC 6 cut(s) 34, 448, 639, 658, 986, 1068
Sau3AI GATC 8 cut(s) 5, 22, 229, 436, 629, 700, 728, 786
Sau96I GGNCC 1 cut(s) 577
SchI GAGTC 7 cut(s) 49, 113, 256, 463, 613, 673, 808
ScrFI CCNGG 1 cut(s) 310
SduI GDGCHC 1 cut(s) 1088
SfaNI GCATC 1 cut(s) 1036
Sfr274I CTCGAG 4 cut(s) 37, 244, 451, 661
SgsI GGCGCGCC 1 cut(s) 1055
SinI GGWCC 1 cut(s) 577
SlaI CTCGAG 4 cut(s) 37, 244, 451, 661
SmiMI CAYNNNNRTG 5 cut(s) 206, 413, 623, 936, 1132
StyD4I CCNGG 1 cut(s) 308
TaiI ACGT 1 cut(s) 1183
TaqI TCGA 6 cut(s) 38, 245, 452, 662, 968, 1110
TatI WGTACW 1 cut(s) 872
TscAI CASTG 1 cut(s) 1084
TseI GCWGC 6 cut(s) 33, 447, 638, 657, 985, 1067
TspDTI ATGAA 5 cut(s) 224, 431, 607, 611, 920
TspRI CASTG 1 cut(s) 1084
VneI GTGCAC 1 cut(s) 1084
VpaK11BI GGWCC 1 cut(s) 577
VspI ATTAAT 4 cut(s) 135, 342, 549, 759
XapI RAATTY 3 cut(s) 780, 878, 1171
XhoI CTCGAG 4 cut(s) 37, 244, 451, 661
XmiI GTMKAC 1 cut(s) 1110
XmnI GAANNNNTTC 2 cut(s) 465, 978
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.