Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr04

Orthogroup ID

OG0009832

Physical Location & Seq

Genomic Coordinates Forward (+)

10411574 .. 10422336

Gene Structure 10763 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

MD04G1077000.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 2769 bp

ATGGCGGCGTCGGGCGGGTATAATTACCGAGATGGCGGTCAGAGGGGAGCTTTGAGGGTGGATAGGCCTCTCTCTGTAAATTCCAACCCCAAGTCTTCTGTCAAATCCAAGTCTTTGCCTGGTTCTGGGCCCCGCCGGAACAGCACCGGCTCCACCGGCGGCGGCGGCGGTTCTGCTGGAGCTGCTGCTGCTAAAAATGATAATACAGGAGTTCTTGGAAGAGTCCGAGTAGCTGTAAGATTGCGACCACATAATGATGAGGAAATGGTTGCAGATGCTGATTTTGCGGACTGCGTAGAATTGCAACCAGAGCTAAAAAGGTTGAAACTTCGGAAGAACAATTGGGACACAGATACTTATGAATTCGATGAAGTGCTCACTGAGTTTGCATCCCAAAAGCGTGTTTATGAAGTAGTGGCAAAGCCTGTGGTTGAGAGTGTTTTGGACGGTTACAATGGAACGGTCATGGCATATGGCCAGACCGGTACAGGGAAAACATATACTCTTGGACGACTTGGAGAGGAAGACACTGCTGCTCGTGGAATTATGGTTCGTGCTATGGAGGATATTTTAGCAGATGTATCTCTGGAGTCTGATTCTTTGTCCGTCTCGTATTTGCAGCTTTACATGGAAACCATACAGGACCTACTTGATCCTGCGAACGATAACATTTCGATTGTGGAAGACCCAAAGACTGGTGATGTTTCAGTACCAGGGGCTAGCCTAGTCGAAATCAGGGACCAGCAGAGTTTTGTAGAACTACTAAGATTAGGGGAAGCTCACCGCTTTGCTACAAATACAAAATTAAATACTGAATCTTCCCGTAGTCATGCTATTCTGATGGTAAACGTAAAGAAGTCTGTCAAGGGAAGAGATTCGGCTCTTTCAAGCCAAAATGGGAACAATTCTCACGTGGTTAAAAGTCTAAAGGCTCCTGTTGTTCGAAAGAGCAAGTTGGTTGTGGTGGATCTTGCTGGTTCAGAGCGCATTGACAAATCAGGAAGTGAAGGACATACACTAGAAGAAGCCAAATCAATTAATCTCTCCTTAAGTGCACTGGGAAAGTGTATTAATGCATTGGCAGAGAATAGTTCACATGTTCCAGTCCGAGATTCAAAACTCACAAGATTGCTTAGGGATTCATTTGGAGGCACAGCAAGAACTTCACTGGTTATTACTATTGGTCCATCCCCACGTCATCGAGGAGAGACAGCTAGCACCATCATGTTTGGACAGCGGGCTATGAAAGTGGAAAATATGTTGAAATTGAAGGAAGAATTTGACTACAAAAGTTTGGCCAGGAGGCTAGATATACAATTAGACAAAATCATTGCAGAACATGAAAGGCAGCAGAAAGTATTTGAGGATGAGATGGAAAGAATAAGCATACAAGCACAAAATCGGATCTCAGATGTTGAAAGGAACTATGCAGACGCACTAGAGAAGGAAAGATTGAAATATCAGAAAGACTATATGGAATCAATAAAGAAGCTTGAAGAGCAGTTGGTGATTACTCAGAAAAAGCATGGCAGTGAAAAAATCACAAATGGACACGAGGACAATGGCTCTAACGTGACGTCAAGCAAGGAGAGTTGCAGGCTGCATGTTGAGGAAATTGACGACCTAACAAAGTTGCTCCAGAAAGAGACCATGCTTAGGAAGGCTGCTGAAGAGGAAGTGCAAAATCTTAAAAGTCAATTGACTCAGTGGAAAAGGTCAGAGGCAGCTGGAAACTCCGAGATCTTAAAACTTCGTAAGATGTTGGAAGATGAGGAACATCAGAAAGAGAAACTTGAAGGAGAAATAGCAAGACTACACAGTCAATTGTTGCAACTAAGTTTTGAAGCTGATGAGACAAGTCAACAACTAGACAGGAGTGGGCCGGGAAAAATTGGTGGTCTTGAGTCCCTTATGACTCAAGTTAGGCAACCGCAGCTTAAAGATTCAGGAAATGGGGAGAAAGCCTCGATAGCAAAACTCTTTGAACAAGTGGGATTGCATAAGATCTTGTCGTTGCTTGAAGCAGAAGATAATGATGTGCGGATTCATGCTGTTAAAGTTGTAGCAAATCTTGCTGCTGAAGAAACAAATCAGGAGAGAATAGTAGAAGCTGGTGGTCTTACATCCTTGTTGATGCTACTTGAAAACTCTGAGGATGAAACCATACATAGAGTTGCTGCTGGTGCAATCGCAAATCTAGCGATGAATGAAACCAACCAGGAGCTGATAATGACTCAAGGGGGAATTAGTTTGTTATCTATGACAGCAGCCCACGGTAAAGATCCCCAAACCCTTCGAATGGTTGCTGGTGCCATTGCTAATCTATGTGGAAACGATAAATTACAGAATAAGCTACGGGGTGAAGGTGGCATCAAGGCGTTGCTAGGAATGGTCAGATGTGGACATCCTGATGTTCTTGCACAAGTTGCTCGAGGAATAGCAAACTTTGCAAAATGCGAGTCTAGAGCATCTACACAAGGTACCAAGACTGGAAAGTCTCTTCTGATTGAAGATGGCGCGCTTGCATGGATTGTGCAGAATGCTAGCAATGACGCCTCACCAATCAAACGCCACATTGAACTTGCACTCTGTCACTTAGCGCAACATGAAGTGAATGCTAAAGACATGATCACTAGTGGTGCCCTGTGGGAGCTGGTCCGCATTTCTCGAGATTGTTCACACGAAGACATAAGAAATCTTGCATATCGAACACTAACTTCGAGCCCTACCTTCCAAGCTGAACTGCGGCGGCTACGGATTGATAACTGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0003774 GO:0003777 GO:0003824 GO:0005575 GO:0005622 GO:0005623 GO:0005856 GO:0005871 GO:0005875 GO:0006928 GO:0007017 GO:0007018 GO:0008150 GO:0009987 GO:0015630 GO:0016462 GO:0016787 GO:0016817 GO:0016818 GO:0016887 GO:0017111 GO:0032991 GO:0043226 GO:0043228 GO:0043229 GO:0043232 GO:0044422 GO:0044424 GO:0044430 GO:0044446 GO:0044464

KEGG Pathways

Metabolic & Signaling

ko04144 ko04728 map04144 map04728

Pfam Domains

Protein Families

Arm Kinesin

Protein Analysis

923

Amino Acids

101.26

Weight (kDa)

6.19

Isoelectric Point (pI)

34.62

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Microtub_bd	PF16796	72 - 217	8.3e-17	Microtubule binding
Kinesin	PF00225	80 - 417	1.8e-95	Kinesin motor domain
ARM_ARMC5	PF24768	687 - 893	5.8e-15	ARMC5
Arm	PF00514	698 - 734	3.4e-06	Armadillo/beta-catenin-like repeat

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0009832)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT1G12430 AT1G12430
fragaria_vesca	FvH4_5g00930 FvH4_5g00930 FvH4_5g00930
malus_domestica	MD04G1077000.v1.1 MD06G1067900.v1.1
prunus_persica	Prupe.5G082300_v2.0.a1 Prupe.5G082300_v2.0.a1
pyrus_communis	pycom04g06770
rosa_chinensis	RchiOBHm_Chr7g0200461
rosa_laevigata	RLG00000003701
rosa_multiflora	Rmu_sc0000617.1_g000027 Rmu_sc0002613.1_g000001
rosa_roxburghii	Rroxscaffold_3G00255950
rosa_rugosa	Rorug07G0060400
rosa_samantha	Rh7AG185900 Rh7BG189000 Rh7CG196200 Rh7DG190000
rosa_wichuraiana	Rw7G016300

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AasI	GACNNNNNNGTC	2 cut(s)	1818, 2494
AatII	GACGTC	1 cut(s)	1580
Acc65I	GGTACC	1 cut(s)	2480
AccB1I	GGYRCC	3 cut(s)	2309, 2480, 2639
AccB7I	CCANNNNNTGG	1 cut(s)	695
AccII	CGCG	1 cut(s)	2519
AclWI	GGATC	4 cut(s)	647, 975, 1412, 2276
AcoI	YGGCCR	2 cut(s)	475, 1296
AcsI	RAATTY	3 cut(s)	79, 362, 1277
AcuI	CTGAAG	2 cut(s)	1689, 2100
AcvI	CACGTG	1 cut(s)	913
AcyI	GRCGYC	3 cut(s)	8, 1577, 2553
AfaI	GTAC	3 cut(s)	487, 711, 2482
AfiI	CCNNNNNNNGG	5 cut(s)	125, 489, 695, 1656, 2299
AflII	CTTAAG	1 cut(s)	1048
AflIII	ACRYGT	1 cut(s)	1096
AgeI	ACCGGT	1 cut(s)	482
AhlI	ACTAGT	1 cut(s)	2633
AjiI	CACGTC	1 cut(s)	1196
AjnI	CCWGG	4 cut(s)	118, 712, 1298, 2217
AloI	GAACNNNNNNTCC	2 cut(s)	534, 566
Alw21I	GWGCWC	2 cut(s)	378, 1057
Alw26I	GTCTC	5 cut(s)	613, 1202, 1640, 1847, 2502
Alw44I	GTGCAC	1 cut(s)	1053
AlwI	GGATC	4 cut(s)	647, 975, 1412, 2276
AlwNI	CAGNNNCTG	2 cut(s)	278, 2224
Ama87I	CYCGRG	2 cut(s)	2430, 2667
AoxI	GGCC	5 cut(s)	65, 128, 475, 1296, 1880
ApaI	GGGCCC	1 cut(s)	132
ApaLI	GTGCAC	1 cut(s)	1053
ApoI	RAATTY	3 cut(s)	79, 362, 1277
ArsI	GACNNNNNNTTYG	2 cut(s)	2444, 2476
AseI	ATTAAT	2 cut(s)	1038, 1071
AsiGI	ACCGGT	1 cut(s)	482
Asp700I	GAANNNNTTC	1 cut(s)	874
Asp718I	GGTACC	1 cut(s)	2480
AspLEI	GCGC	4 cut(s)	987, 2519, 2521, 2602
AspS9I	GGNCC	7 cut(s)	128, 129, 643, 739, 1184, 1880, 2656
AsuC2I	CCSGG	1 cut(s)	1884
AsuHPI	GGTGA	5 cut(s)	710, 773, 1519, 2372, 2550
AsuII	TTCGAA	2 cut(s)	943, 2296
AsuNHI	GCTAGC	3 cut(s)	719, 1214, 2543
AvaI	CYCGRG	2 cut(s)	2430, 2667
AvaII	GGWCC	4 cut(s)	643, 739, 1184, 2656
BaeGI	GKGCMC	3 cut(s)	132, 1057, 2644
BalI	TGGCCA	2 cut(s)	477, 1298
BanI	GGYRCC	3 cut(s)	2309, 2480, 2639
BanII	GRGCYC	2 cut(s)	132, 2726
BauI	CACGAG	2 cut(s)	537, 1553
BbrPI	CACGTG	1 cut(s)	913
BbsI	GAAGAC	4 cut(s)	87, 531, 690, 2691
Bbv12I	GWGCWC	2 cut(s)	378, 1057
BccI	CCATC	6 cut(s)	26, 835, 1195, 1229, 1366, 2507
BciT130I	CCWGG	4 cut(s)	120, 714, 1300, 2219
BclI	TGATCA	1 cut(s)	2628
BcnI	CCSGG	1 cut(s)	1884
BcoDI	GTCTC	5 cut(s)	613, 1202, 1640, 1847, 2502
BcuI	ACTAGT	1 cut(s)	2633
BfrI	CTTAAG	1 cut(s)	1048
BglII	AGATCT	2 cut(s)	1740, 2004
Bme1390I	CCNGG	5 cut(s)	120, 714, 1300, 1884, 2219
Bme18I	GGWCC	4 cut(s)	643, 739, 1184, 2656
BmeT110I	CYCGRG	2 cut(s)	2430, 2667
BmgBI	CACGTC	1 cut(s)	1196
BmgT120I	GGNCC	7 cut(s)	128, 129, 643, 739, 1184, 1880, 2656
BmiI	GGNNCC	8 cut(s)	130, 131, 151, 740, 933, 2311, 2482, 2641
BmrFI	CCNGG	5 cut(s)	120, 714, 1300, 1884, 2219
BmrI	ACTGGG	1 cut(s)	1067
BmsI	GCATC	5 cut(s)	265, 398, 2124, 2379, 2477
BmtI	GCTAGC	3 cut(s)	723, 1218, 2547
BmuI	ACTGGG	1 cut(s)	1067
BpiI	GAAGAC	4 cut(s)	87, 531, 690, 2691
BplI	GAGNNNNNCTC	2 cut(s)	1949, 1981
BpmI	CTGGAG	3 cut(s)	198, 608, 1622
Bpu10I	CCTNAGC	2 cut(s)	1133, 1655
Bpu14I	TTCGAA	2 cut(s)	943, 2296
BpuEI	CTTGAG	3 cut(s)	1902, 1922, 2220
BpuMI	CCSGG	1 cut(s)	1884
BsaAI	YACGTR	1 cut(s)	913
BsaHI	GRCGYC	3 cut(s)	8, 1577, 2553
BsaI	GGTCTC	1 cut(s)	1640
BsaJI	CCNNGG	2 cut(s)	713, 2272
BsaWI	WCCGGW	1 cut(s)	482
Bsc4I	CCNNNNNNNGG	5 cut(s)	125, 489, 695, 1656, 2299
Bse118I	RCCGGY	3 cut(s)	146, 155, 482
Bse1I	ACTGG	5 cut(s)	700, 1062, 1103, 1173, 2494
Bse3DI	GCAATG	3 cut(s)	1329, 2313, 2554
BseBI	CCWGG	4 cut(s)	120, 714, 1300, 2219
BseDI	CCNNGG	2 cut(s)	713, 2272
BseGI	GGATG	6 cut(s)	389, 1187, 1372, 2123, 2161, 2404
BseLI	CCNNNNNNNGG	5 cut(s)	125, 489, 695, 1656, 2299
BseMI	GCAATG	3 cut(s)	1329, 2313, 2554
BseMII	CTCAG	5 cut(s)	372, 1422, 1529, 1718, 2142
BseNI	ACTGG	5 cut(s)	700, 1062, 1103, 1173, 2494
BsePI	GCGCGC	1 cut(s)	2517
BseRI	GAGGAG	1 cut(s)	1218
BseSI	GKGCMC	3 cut(s)	132, 1057, 2644
BsgI	GTGCAG	1 cut(s)	2555
Bsh1236I	CGCG	1 cut(s)	2519
BshFI	GGCC	5 cut(s)	67, 130, 477, 1298, 1882
BshNI	GGYRCC	3 cut(s)	2309, 2480, 2639
BshTI	ACCGGT	1 cut(s)	482
BsiHKAI	GWGCWC	2 cut(s)	378, 1057
BsiHKCI	CYCGRG	2 cut(s)	2430, 2667
BsiSI	CCGG	5 cut(s)	136, 147, 156, 483, 1883
BslFI	GGGAC	3 cut(s)	359, 752, 1891
BslI	CCNNNNNNNGG	5 cut(s)	125, 489, 695, 1656, 2299
BsmAI	GTCTC	5 cut(s)	613, 1202, 1640, 1847, 2502
BsmBI	CGTCTC	1 cut(s)	613
BsmFI	GGGAC	3 cut(s)	359, 752, 1891
BsmI	GAATGC	2 cut(s)	2545, 2620
BsnI	GGCC	5 cut(s)	67, 130, 477, 1298, 1882
Bso31I	GGTCTC	1 cut(s)	1640
BsoBI	CYCGRG	2 cut(s)	2430, 2667
Bsp119I	TTCGAA	2 cut(s)	943, 2296
Bsp120I	GGGCCC	1 cut(s)	128
Bsp1286I	GDGCHC	5 cut(s)	132, 378, 1057, 2644, 2726
Bsp143I	GATC	7 cut(s)	652, 967, 1404, 1740, 2004, 2281, 2628
BspANI	GGCC	5 cut(s)	67, 130, 477, 1298, 1882
BspCNI	CTCAG	5 cut(s)	373, 1421, 1528, 1717, 2143
BspFNI	CGCG	1 cut(s)	2519
BspLI	GGNNCC	8 cut(s)	130, 131, 151, 740, 933, 2311, 2482, 2641
BspOI	GCTAGC	3 cut(s)	723, 1218, 2547
BspPI	GGATC	4 cut(s)	647, 975, 1412, 2276
BspQI	GCTCTTC	1 cut(s)	1491
BspT104I	TTCGAA	2 cut(s)	943, 2296
BspT107I	GGYRCC	3 cut(s)	2309, 2480, 2639
BspTI	CTTAAG	1 cut(s)	1048
BspTNI	GGTCTC	1 cut(s)	1640
BsrDI	GCAATG	3 cut(s)	1329, 2313, 2554
BsrFI	RCCGGY	3 cut(s)	146, 155, 482
BsrI	ACTGG	5 cut(s)	700, 1062, 1103, 1173, 2494
BssAI	RCCGGY	3 cut(s)	146, 155, 482
BssECI	CCNNGG	2 cut(s)	713, 2272
BssHII	GCGCGC	1 cut(s)	2517
BssMI	GATC	7 cut(s)	652, 967, 1404, 1740, 2004, 2281, 2628
BssNI	GRCGYC	3 cut(s)	8, 1577, 2553
BssSI	CACGAG	2 cut(s)	537, 1553
Bst2BI	CACGAG	2 cut(s)	537, 1553
Bst2UI	CCWGG	4 cut(s)	120, 714, 1300, 2219
Bst4CI	ACNGT	4 cut(s)	449, 463, 1820, 2276
Bst6I	CTCTTC	5 cut(s)	214, 865, 1491, 1665, 2505
BstACI	GRCGYC	3 cut(s)	8, 1577, 2553
BstAFI	CTTAAG	1 cut(s)	1048
BstAPI	GCANNNNNTGC	3 cut(s)	2072, 2426, 2447
BstBAI	YACGTR	1 cut(s)	913
BstBI	TTCGAA	2 cut(s)	943, 2296
BstC8I	GCNNGC	7 cut(s)	721, 1216, 1239, 1598, 2519, 2523, 2545
BstDSI	CCRYGG	1 cut(s)	2272
BstF5I	GGATG	6 cut(s)	389, 1187, 1372, 2123, 2161, 2404
BstFNI	CGCG	1 cut(s)	2519
BstHHI	GCGC	4 cut(s)	987, 2519, 2521, 2602
BstKTI	GATC	7 cut(s)	655, 970, 1407, 1743, 2007, 2284, 2631
BstMAI	GTCTC	5 cut(s)	613, 1202, 1640, 1847, 2502
BstMBI	GATC	7 cut(s)	652, 967, 1404, 1740, 2004, 2281, 2628
BstNI	CCWGG	4 cut(s)	120, 714, 1300, 2219
BstNSI	RCATGY	2 cut(s)	1100, 1607
BstSCI	CCNGG	5 cut(s)	118, 712, 1298, 1882, 2217
BstSLI	GKGCMC	3 cut(s)	132, 1057, 2644
BstUI	CGCG	1 cut(s)	2519
BstV2I	GAAGAC	4 cut(s)	87, 531, 690, 2691
BstX2I	RGATCY	5 cut(s)	967, 1404, 1740, 2004, 2281
BstYI	RGATCY	5 cut(s)	967, 1404, 1740, 2004, 2281
BsuRI	GGCC	5 cut(s)	67, 130, 477, 1298, 1882
BtgI	CCRYGG	1 cut(s)	2272
BtgZI	GCGATG	1 cut(s)	2216
BtrI	CACGTC	1 cut(s)	1196
BtsCI	GGATG	6 cut(s)	389, 1187, 1372, 2123, 2161, 2404
BtsI	GCAGTG	2 cut(s)	528, 1537
BtsIMutI	CAGTG	6 cut(s)	378, 528, 1055, 1166, 1537, 1712
Cac8I	GCNNGC	7 cut(s)	721, 1216, 1239, 1598, 2519, 2523, 2545
CaiI	CAGNNNCTG	2 cut(s)	278, 2224
CfoI	GCGC	4 cut(s)	987, 2519, 2521, 2602
Cfr10I	RCCGGY	3 cut(s)	146, 155, 482
Cfr13I	GGNCC	7 cut(s)	128, 129, 643, 739, 1184, 1880, 2656
CseI	GACGC	2 cut(s)	1442, 2561
Csp6I	GTAC	3 cut(s)	486, 710, 2481
CspAI	ACCGGT	1 cut(s)	482
CspCI	CAANNNNNGTGG	2 cut(s)	408, 443
CviQI	GTAC	3 cut(s)	486, 710, 2481
DpnI	GATC	7 cut(s)	654, 969, 1406, 1742, 2006, 2283, 2630
DpnII	GATC	7 cut(s)	652, 967, 1404, 1740, 2004, 2281, 2628
DrdI	GACNNNNNNGTC	2 cut(s)	1818, 2494
DseDI	GACNNNNNNGTC	2 cut(s)	1818, 2494
EaeI	YGGCCR	2 cut(s)	475, 1296
Eam1104I	CTCTTC	5 cut(s)	214, 865, 1491, 1665, 2505
EarI	CTCTTC	5 cut(s)	214, 865, 1491, 1665, 2505
Eco147I	AGGCCT	1 cut(s)	67
Eco24I	GRGCYC	2 cut(s)	132, 2726
Eco31I	GGTCTC	1 cut(s)	1640
Eco47I	GGWCC	4 cut(s)	643, 739, 1184, 2656
Eco57I	CTGAAG	2 cut(s)	1689, 2100
Eco72I	CACGTG	1 cut(s)	913
Eco88I	CYCGRG	2 cut(s)	2430, 2667
EcoO109I	RGGNCCY	2 cut(s)	129, 643
EcoRI	GAATTC	1 cut(s)	362
EcoRII	CCWGG	4 cut(s)	118, 712, 1298, 2217
EcoT22I	ATGCAT	1 cut(s)	1078
EcoT38I	GRGCYC	2 cut(s)	132, 2726
Esp3I	CGTCTC	1 cut(s)	613
FalI	AAGNNNNNCTT	2 cut(s)	1776, 1808
FaqI	GGGAC	3 cut(s)	359, 752, 1891
FauI	CCCGC	3 cut(s)	8, 140, 1230
FauNDI	CATATG	1 cut(s)	472
FbaI	TGATCA	1 cut(s)	2628
FokI	GGATG	6 cut(s)	376, 1174, 1379, 2110, 2168, 2391
FriOI	GRGCYC	2 cut(s)	132, 2726
GlaI	GCGC	4 cut(s)	986, 2518, 2520, 2601
GsuI	CTGGAG	3 cut(s)	198, 608, 1622
HaeIII	GGCC	5 cut(s)	67, 130, 477, 1298, 1882
HapII	CCGG	5 cut(s)	136, 147, 156, 483, 1883
HgaI	GACGC	2 cut(s)	1442, 2561
HhaI	GCGC	4 cut(s)	987, 2519, 2521, 2602
Hin1I	GRCGYC	3 cut(s)	8, 1577, 2553
Hin6I	GCGC	4 cut(s)	985, 2517, 2519, 2600
HinP1I	GCGC	4 cut(s)	985, 2517, 2519, 2600
HincII	GTYRAC	1 cut(s)	1862
HindII	GTYRAC	1 cut(s)	1862
HindIII	AAGCTT	1 cut(s)	1490
HpaII	CCGG	5 cut(s)	136, 147, 156, 483, 1883
HphI	GGTGA	5 cut(s)	710, 773, 1519, 2372, 2550
Hpy166II	GTNNAC	6 cut(s)	847, 1055, 1094, 1862, 2402, 2678
Hpy8I	GTNNAC	6 cut(s)	847, 1055, 1094, 1862, 2402, 2678
Hpy99I	CGWCG	1 cut(s)	13
HpyAV	CCTTC	8 cut(s)	1001, 1264, 1438, 1654, 1790, 2303, 2357, 2740
HpyCH4III	ACNGT	4 cut(s)	449, 463, 1820, 2276
HpyCH4IV	ACGT	5 cut(s)	849, 912, 1195, 1572, 1577
HpySE526I	ACGT	5 cut(s)	849, 912, 1195, 1572, 1577
Hsp92I	GRCGYC	3 cut(s)	8, 1577, 2553
HspAI	GCGC	4 cut(s)	985, 2517, 2519, 2600
KpnI	GGTACC	1 cut(s)	2484
Ksp22I	TGATCA	1 cut(s)	2628
Kzo9I	GATC	7 cut(s)	652, 967, 1404, 1740, 2004, 2281, 2628
LguI	GCTCTTC	1 cut(s)	1491
LmnI	GCTCC	7 cut(s)	47, 155, 179, 937, 1641, 2221, 2650
LweI	GCATC	5 cut(s)	265, 398, 2124, 2379, 2477
MaeII	ACGT	5 cut(s)	849, 912, 1195, 1572, 1577
MaeIII	GTNAC	3 cut(s)	449, 1573, 2591
MalI	GATC	7 cut(s)	654, 969, 1406, 1742, 2006, 2283, 2630
MboI	GATC	7 cut(s)	652, 967, 1404, 1740, 2004, 2281, 2628
MfeI	CAATTG	3 cut(s)	340, 1697, 1823
MflI	RGATCY	5 cut(s)	967, 1404, 1740, 2004, 2281
MhlI	GDGCHC	5 cut(s)	132, 378, 1057, 2644, 2726
MlsI	TGGCCA	2 cut(s)	477, 1298
MluNI	TGGCCA	2 cut(s)	477, 1298
MlyI	GAGTC	7 cut(s)	231, 599, 1696, 1909, 1913, 2227, 2468
MmeI	TCCRAC	2 cut(s)	108, 1743
Mox20I	TGGCCA	2 cut(s)	477, 1298
Mph1103I	ATGCAT	1 cut(s)	1078
MroXI	GAANNNNTTC	1 cut(s)	874
MscI	TGGCCA	2 cut(s)	477, 1298
MseI	TTAA	9 cut(s)	806, 918, 1038, 1049, 1071, 1689, 1745, 1938, 2055
MslI	CAYNNNNRTG	4 cut(s)	255, 1223, 1530, 2409
Msp20I	TGGCCA	2 cut(s)	477, 1298
MspA1I	CMGCKG	2 cut(s)	1237, 1727
MspCI	CTTAAG	1 cut(s)	1048
MspI	CCGG	5 cut(s)	136, 147, 156, 483, 1883
MspR9I	CCNGG	5 cut(s)	120, 714, 1300, 1884, 2219
MunI	CAATTG	3 cut(s)	340, 1697, 1823
Mva1269I	GAATGC	2 cut(s)	2545, 2620
MvaI	CCWGG	4 cut(s)	120, 714, 1300, 2219
MvnI	CGCG	1 cut(s)	2519
NciI	CCSGG	1 cut(s)	1884
NdeI	CATATG	1 cut(s)	472
NdeII	GATC	7 cut(s)	652, 967, 1404, 1740, 2004, 2281, 2628
NheI	GCTAGC	3 cut(s)	719, 1214, 2543
NlaIV	GGNNCC	8 cut(s)	130, 131, 151, 740, 933, 2311, 2482, 2641
NmuCI	GTSAC	2 cut(s)	1573, 2591
NsiI	ATGCAT	1 cut(s)	1078
NspI	RCATGY	2 cut(s)	1100, 1607
NspV	TTCGAA	2 cut(s)	943, 2296
PaeR7I	CTCGAG	2 cut(s)	2430, 2667
PauI	GCGCGC	1 cut(s)	2517
PceI	AGGCCT	1 cut(s)	67
PciI	ACATGT	1 cut(s)	1096
PciSI	GCTCTTC	1 cut(s)	1491
PctI	GAATGC	2 cut(s)	2545, 2620
PdmI	GAANNNNTTC	1 cut(s)	874
PfeI	GAWTC	8 cut(s)	596, 815, 875, 1112, 1139, 1478, 1943, 2044
PflMI	CCANNNNNTGG	1 cut(s)	695
PinAI	ACCGGT	1 cut(s)	482
PleI	GAGTC	7 cut(s)	230, 598, 1696, 1909, 1912, 2227, 2467
PmaCI	CACGTG	1 cut(s)	913
PmlI	CACGTG	1 cut(s)	913
PpsI	GAGTC	7 cut(s)	230, 598, 1696, 1909, 1912, 2227, 2467
Ppu21I	YACGTR	1 cut(s)	913
PpuMI	RGGWCCY	1 cut(s)	643
PscI	ACATGT	1 cut(s)	1096
PshBI	ATTAAT	2 cut(s)	1038, 1071
Psp5II	RGGWCCY	1 cut(s)	643
Psp6I	CCWGG	4 cut(s)	118, 712, 1298, 2217
PspCI	CACGTG	1 cut(s)	913
PspGI	CCWGG	4 cut(s)	118, 712, 1298, 2217
PspN4I	GGNNCC	8 cut(s)	130, 131, 151, 740, 933, 2311, 2482, 2641
PspOMI	GGGCCC	1 cut(s)	128
PspPI	GGNCC	7 cut(s)	128, 129, 643, 739, 1184, 1880, 2656
PspPPI	RGGWCCY	1 cut(s)	643
PspXI	VCTCGAGB	1 cut(s)	2430
PstNI	CAGNNNCTG	2 cut(s)	278, 2224
PsuI	RGATCY	5 cut(s)	967, 1404, 1740, 2004, 2281
PteI	GCGCGC	1 cut(s)	2517
PvuII	CAGCTG	1 cut(s)	1727
RsaI	GTAC	3 cut(s)	487, 711, 2482
RsaNI	GTAC	3 cut(s)	486, 710, 2481
RseI	CAYNNNNRTG	4 cut(s)	255, 1223, 1530, 2409
SapI	GCTCTTC	1 cut(s)	1491
SaqAI	TTAA	9 cut(s)	806, 918, 1038, 1049, 1071, 1689, 1745, 1938, 2055
Sau3AI	GATC	7 cut(s)	652, 967, 1404, 1740, 2004, 2281, 2628
Sau96I	GGNCC	7 cut(s)	128, 129, 643, 739, 1184, 1880, 2656
SchI	GAGTC	7 cut(s)	231, 599, 1696, 1909, 1913, 2227, 2468
ScrFI	CCNGG	5 cut(s)	120, 714, 1300, 1884, 2219
SduI	GDGCHC	5 cut(s)	132, 378, 1057, 2644, 2726
SfaNI	GCATC	5 cut(s)	265, 398, 2124, 2379, 2477
Sfr274I	CTCGAG	2 cut(s)	2430, 2667
SfuI	TTCGAA	2 cut(s)	943, 2296
SgrAI	CRCCGGYG	1 cut(s)	155
SinI	GGWCC	4 cut(s)	643, 739, 1184, 2656
SlaI	CTCGAG	2 cut(s)	2430, 2667
SmiMI	CAYNNNNRTG	4 cut(s)	255, 1223, 1530, 2409
SmlI	CTYRAG	6 cut(s)	1048, 1901, 1917, 2235, 2430, 2667
SmoI	CTYRAG	6 cut(s)	1048, 1901, 1917, 2235, 2430, 2667
SpeI	ACTAGT	1 cut(s)	2633
SseBI	AGGCCT	1 cut(s)	67
StuI	AGGCCT	1 cut(s)	67
StyD4I	CCNGG	5 cut(s)	118, 712, 1298, 1882, 2217
TaaI	ACNGT	4 cut(s)	449, 463, 1820, 2276
TaiI	ACGT	5 cut(s)	852, 915, 1198, 1575, 1580
TauI	GCSGC	6 cut(s)	8, 162, 165, 168, 2749, 2752
TfiI	GAWTC	8 cut(s)	596, 815, 875, 1112, 1139, 1478, 1943, 2044
Tru1I	TTAA	9 cut(s)	806, 918, 1038, 1049, 1071, 1689, 1745, 1938, 2055
Tru9I	TTAA	9 cut(s)	806, 918, 1038, 1049, 1071, 1689, 1745, 1938, 2055
TscAI	CASTG	6 cut(s)	385, 535, 1062, 1173, 1537, 1712
TseFI	GTSAC	2 cut(s)	1573, 2591
Tsp45I	GTSAC	2 cut(s)	1573, 2591
TspGWI	ACGGA	1 cut(s)	595
TspRI	CASTG	6 cut(s)	385, 535, 1062, 1173, 1537, 1712
Van91I	CCANNNNNTGG	1 cut(s)	695
Vha464I	CTTAAG	1 cut(s)	1048
VneI	GTGCAC	1 cut(s)	1053
VpaK11BI	GGWCC	4 cut(s)	643, 739, 1184, 2656
VspI	ATTAAT	2 cut(s)	1038, 1071
XapI	RAATTY	3 cut(s)	79, 362, 1277
XbaI	TCTAGA	1 cut(s)	2462
XceI	RCATGY	2 cut(s)	1100, 1607
XhoI	CTCGAG	2 cut(s)	2430, 2667
XmnI	GAANNNNTTC	1 cut(s)	874
ZraI	GACGTC	1 cut(s)	1578
Zsp2I	ATGCAT	1 cut(s)	1078

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.