Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr06

Orthogroup ID

OG0009832

Physical Location & Seq

Genomic Coordinates Reverse (-)

15264538 .. 15273670

Gene Structure 9133 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

MD06G1067900.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 2766 bp

ATGGCGGCGTCGGGCGGGTATAATTACCGAGATGGCAGTCAGAGGGGTGCTTTGAGGGTGGATAGGCCTCTCTCTGTAAATTCCAATCCCAAGTCTTCTGTCAAATCCAAGTCTTTGCCTGCTTCTGGGCCACGCCGGAACAGCACCGGCTCCACTGGCGGCGGTGGCGCTACTGGAGCTGCTGCTTCTAAGAATGATAATACAGGAGTTCTTGGAAGAGTTCGAGTAGCTCTAAGATTGCGACCACGTAATGAGGAGGAAATGGTTGCAGATGCTGATTTTGCGGACTGTGTAGAGTTGCAACCAGAGCTAAAAAGGTTGAAACTTCGGAAAAACAATTGGGACACAGATACTTATGAGTTCGATGAAGTGCTCACAGAGTTTGCATCCCAAAAACGTGTTTATGAAGTAGTGGCAAAGCCTGTTGTTGAGAGTGTTCTGGAAGGTTACAATGGAACGGTCATGGCATATGGTCAGACCGGTACAGGGAAAACATATACTCTTGGACGACTTGGAGAGGAAGACACTGCTGCTCGTGGAATAATGGTTCGTGCTATGGAGGATATTTTAGCAGATGTATCTCTGGAGTCCGATTCTTTGTCAGTCTCGTATTTTCAGCTTTACATGGAAACCATACAGGACCTACTTGATCCTGCTAATGACAACATTTCTATAGTGGAAGACCCAAAGACTGGTGATGTCTCAGTACCAGGGGCTAGCCTAGTCGAAATCAGGGACCAGCAGAGTTTCGTAGAACTACTAAGATTAGGGGAAGGCCACCTCTTTGCTTCAAATACAAAATTGAATACTGAATCTTCCCGCAGTCATGCTATTCTGATGGTAAATGTAAAGAAGTCTGTGAAGGGAAGAGATTCAGCTCTTTCAAGCCAAAATGGGAACAATTCTCACATGGTAAAAAGTCTAAAGGCTCCTGTTGTTCGAAAGAGCAAGCTGGTTGTAGTGGATCTTGCTGGTTCAGAGCGCATTGACAAATCAGGAAGTGAAGGACATACGCTAGAAGAAGCCAAATCAATCAATCTCTCCTTAAGTGCACTGGGAAAGTGCATTAATGCATTGGCAGAGAATAGTTCACATGTTCCAGTTCGAGATTCCAAACTCACAAGATTGCTTCGGGATTCATTTGGAGGCACGGCAAGAACTTCACTAGTTGTTACTATTGGTCCATCCCCACGTCATCGAGGAGAGACAGCTAGCACTATTATGTTTGGACAGCGGGCTATGAAAGTGGAAAATATGTTGAAATTGAAGGAAGAATTCGACTACAAAAGTTTGGCCAGGAGGCTAGATATACAATTAGACAAAATCATTGCAGAACATGAGAGGCAGCAGAAAGTATTTGAGGATGAGATGGAAAGAGTAAGCATAGAAGCACAAAATCGGATCTCAGAGGTTGAAAGGAACTACACAGATGCACTGGAGAAGGAAAGATTGAAATATCAGAAAGACTATATGGAATCAATAAAGAAGCTCGAAGAGCAGTTGGTGATGACTCAGAGAAAGCATGGTAGTCAAAAAATCACAAATGGACATGAGGATGATGGCTTTAACGTGGCATCCAGCAAAGAGAGTTGTAGGCTGCATGTTGAGGAAATTGACGACCTAACAAAGTTGCTCCAGAAAGAGACTATGCTTAGGAAGGCTGTTGAGGAGGAAGTGCATAATCTTAAAAGTCAATTGACTCAGTGGAAAAGGTCAGAGGCAGCTGGAAATTCGGAGATCTTAAAGCTTCGTAAGATGTTGGAAGATGAGGAACATCAGAAAGAGAAACTTGAAGGAGAAATAGCAAGACTACACAGTCAGTTGTTGCAACGAAGTTTTGAAGCTGATGAGACAAGTCAACAACTAGACAGGAGTGGGGCCGAAAAAATTGGTGCTCTTGAGTCCCTTATGACTCAAGTTAGGAAACCGCAGCTTAAAGATTCAGGAAATGAAGAGAAAGCCTCGATAGCAAAACTCTTTGAGCAAGTGGGATTGCAAAAGATCTTGTCATTGCTTGAAGCAGAAGATAATGATGTGAGGATTCATGCTGTTAAAGTTGTAGCAAATCTTGCTGCTGAAGAAGCAAATCAGGAGAAAATCGTAGAAGCTGGTGGTCTTACATCCTTGTTGATGCTACTTAAAAACTCTGAGGATGAAACCATACATAGAGTTGCAGCTGGTGCAATCGCAAATCTAGCTATGAATGAAACCAACCAGGAGCTGATTATGACTCAAGGGGGAATTAGTTTGTTATCTATGACAGCAGCCAACGGTGAAGATCCCCAAACTCTTCGAATGGTTGCTGGAGCCATTGCTAATCTATGCGGAAATGATAAATTACAGAATAAGATAAGGGGTGAAGGTGGCATCAAGGCGTTGTTAGGAATGGTCAGATGTGGACATCCTGATGTTCTTGCACAAGTTGCTCGAGGAATAGCAAACTTCGCAAAATGCGAGTCTAGAGCATCTACACAGGGTACCAAGACTGGAAAGTCTCTTCTGATTGAAGATGGCGCAGTTGCATGGATTGTACAGAATGCTAACAATGACGCCGCACCAATCAAACGCCATGTTGAACTTGCACTCTGTCATTTAGCGCAACATGAAGTGAATGCTAAAGACATGATCACTAGCGGTGCCCTGTGGGAGCTGGTCCGCATTTCTAGAGATTGTTCACGCGAAGACATAAGAACTCTTGCATATCGAACACTAACTTCGAGCCCTACCTTCCAAGCTGAACTAAGGCGGCTACGGATTGATAACTAA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0003774 GO:0003777 GO:0003824 GO:0005575 GO:0005622 GO:0005623 GO:0005856 GO:0005871 GO:0005875 GO:0006928 GO:0007017 GO:0007018 GO:0008150 GO:0009987 GO:0015630 GO:0016462 GO:0016787 GO:0016817 GO:0016818 GO:0016887 GO:0017111 GO:0032991 GO:0043226 GO:0043228 GO:0043229 GO:0043232 GO:0044422 GO:0044424 GO:0044430 GO:0044446 GO:0044464

KEGG Pathways

Metabolic & Signaling

ko04144 ko04728 map04144 map04728

Pfam Domains

Protein Families

Arm Kinesin

Protein Analysis

922

Amino Acids

101.54

Weight (kDa)

6.12

Isoelectric Point (pI)

35.02

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Microtub_bd	PF16796	72 - 216	2.1e-15	Microtubule binding
Kinesin	PF00225	79 - 416	1.9e-94	Kinesin motor domain
ARM_ARMC5	PF24768	686 - 892	7.3e-16	ARMC5
Arm	PF00514	696 - 733	1e-06	Armadillo/beta-catenin-like repeat

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

Style Settings

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0009832)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT1G12430 AT1G12430
fragaria_vesca	FvH4_5g00930 FvH4_5g00930 FvH4_5g00930
malus_domestica	MD04G1077000.v1.1 MD06G1067900.v1.1
prunus_persica	Prupe.5G082300_v2.0.a1 Prupe.5G082300_v2.0.a1
pyrus_communis	pycom04g06770
rosa_chinensis	RchiOBHm_Chr7g0200461
rosa_laevigata	RLG00000003701
rosa_multiflora	Rmu_sc0000617.1_g000027 Rmu_sc0002613.1_g000001
rosa_roxburghii	Rroxscaffold_3G00255950
rosa_rugosa	Rorug07G0060400
rosa_samantha	Rh7AG185900 Rh7BG189000 Rh7CG196200 Rh7DG190000
rosa_wichuraiana	Rw7G016300

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AasI	GACNNNNNNGTC	2 cut(s)	1815, 2491
Acc65I	GGTACC	1 cut(s)	2477
AccB1I	GGYRCC	2 cut(s)	2477, 2636
AccB7I	CCANNNNNTGG	1 cut(s)	692
AccII	CGCG	1 cut(s)	2679
AclWI	GGATC	4 cut(s)	644, 972, 1409, 2273
AcoI	YGGCCR	1 cut(s)	1293
AcsI	RAATTY	3 cut(s)	79, 1274, 1729
AcuI	CTGAAG	1 cut(s)	2097
AcyI	GRCGYC	2 cut(s)	8, 2550
AfaI	GTAC	4 cut(s)	484, 708, 2479, 2532
AfiI	CCNNNNNNNGG	3 cut(s)	125, 486, 692
AflII	CTTAAG	1 cut(s)	1045
AflIII	ACRYGT	2 cut(s)	397, 1093
AgeI	ACCGGT	1 cut(s)	479
AhlI	ACTAGT	1 cut(s)	1165
AjiI	CACGTC	1 cut(s)	1193
AjnI	CCWGG	3 cut(s)	709, 1295, 2214
AloI	GAACNNNNNNTCC	2 cut(s)	531, 563
Alw21I	GWGCWC	3 cut(s)	375, 1054, 1897
Alw26I	GTCTC	6 cut(s)	610, 706, 1199, 1637, 1844, 2499
Alw44I	GTGCAC	1 cut(s)	1050
AlwI	GGATC	4 cut(s)	644, 972, 1409, 2273
AlwNI	CAGNNNCTG	2 cut(s)	275, 2221
Ama87I	CYCGRG	1 cut(s)	2427
AoxI	GGCC	5 cut(s)	65, 128, 775, 1293, 1878
ApaLI	GTGCAC	1 cut(s)	1050
ApoI	RAATTY	3 cut(s)	79, 1274, 1729
ArsI	GACNNNNNNTTYG	2 cut(s)	2441, 2473
AseI	ATTAAT	1 cut(s)	1068
AsiGI	ACCGGT	1 cut(s)	479
Asp700I	GAANNNNTTC	1 cut(s)	871
Asp718I	GGTACC	1 cut(s)	2477
AspLEI	GCGC	4 cut(s)	170, 984, 2516, 2599
AspS9I	GGNCC	6 cut(s)	128, 640, 736, 1181, 1878, 2653
AsuHPI	GGTGA	4 cut(s)	707, 1516, 2285, 2369
AsuII	TTCGAA	2 cut(s)	940, 2293
AsuNHI	GCTAGC	2 cut(s)	716, 1211
AvaI	CYCGRG	1 cut(s)	2427
AvaII	GGWCC	4 cut(s)	640, 736, 1181, 2653
BaeGI	GKGCMC	2 cut(s)	1054, 2641
BalI	TGGCCA	1 cut(s)	1295
BanI	GGYRCC	2 cut(s)	2477, 2636
BanII	GRGCYC	1 cut(s)	2723
BauI	CACGAG	1 cut(s)	534
BbsI	GAAGAC	4 cut(s)	87, 528, 687, 2688
Bbv12I	GWGCWC	3 cut(s)	375, 1054, 1897
BccI	CCATC	6 cut(s)	26, 832, 1192, 1363, 1553, 2504
BceAI	ACGGC	1 cut(s)	1167
BciT130I	CCWGG	3 cut(s)	711, 1297, 2216
BclI	TGATCA	1 cut(s)	2625
BcoDI	GTCTC	6 cut(s)	610, 706, 1199, 1637, 1844, 2499
BcuI	ACTAGT	1 cut(s)	1165
BfmI	CTRYAG	1 cut(s)	672
BfoI	RGCGCY	1 cut(s)	171
BfrI	CTTAAG	1 cut(s)	1045
BglII	AGATCT	2 cut(s)	1737, 2001
Bme1390I	CCNGG	3 cut(s)	711, 1297, 2216
Bme18I	GGWCC	4 cut(s)	640, 736, 1181, 2653
BmeT110I	CYCGRG	1 cut(s)	2427
BmgBI	CACGTC	1 cut(s)	1193
BmgT120I	GGNCC	6 cut(s)	128, 640, 736, 1181, 1878, 2653
BmiI	GGNNCC	7 cut(s)	151, 737, 930, 1879, 2308, 2479, 2638
BmrFI	CCNGG	3 cut(s)	711, 1297, 2216
BmrI	ACTGGG	1 cut(s)	1064
BmsI	GCATC	7 cut(s)	262, 395, 1420, 1583, 2121, 2376, 2474
BmtI	GCTAGC	2 cut(s)	720, 1215
BmuI	ACTGGG	1 cut(s)	1064
BpiI	GAAGAC	4 cut(s)	87, 528, 687, 2688
BplI	GAGNNNNNCTC	2 cut(s)	1946, 1978
BpmI	CTGGAG	5 cut(s)	195, 605, 1457, 1619, 2325
Bpu10I	CCTNAGC	1 cut(s)	1652
Bpu14I	TTCGAA	2 cut(s)	940, 2293
BpuEI	CTTGAG	3 cut(s)	1899, 1919, 2217
BsaAI	YACGTR	1 cut(s)	248
BsaHI	GRCGYC	2 cut(s)	8, 2550
BsaJI	CCNNGG	1 cut(s)	710
BsaWI	WCCGGW	1 cut(s)	479
Bsc4I	CCNNNNNNNGG	3 cut(s)	125, 486, 692
Bse118I	RCCGGY	2 cut(s)	146, 479
Bse1I	ACTGG	7 cut(s)	160, 178, 697, 1059, 1100, 1440, 2491
Bse3DI	GCAATG	3 cut(s)	1326, 2009, 2310
BseBI	CCWGG	3 cut(s)	711, 1297, 2216
BseDI	CCNNGG	1 cut(s)	710
BseGI	GGATG	8 cut(s)	386, 1184, 1369, 1561, 1574, 2120, 2158, 2401
BseLI	CCNNNNNNNGG	3 cut(s)	125, 486, 692
BseMI	GCAATG	3 cut(s)	1326, 2009, 2310
BseMII	CTCAG	5 cut(s)	717, 1419, 1526, 1715, 2139
BseNI	ACTGG	7 cut(s)	160, 178, 697, 1059, 1100, 1440, 2491
BseRI	GAGGAG	3 cut(s)	269, 1215, 1682
BseSI	GKGCMC	2 cut(s)	1054, 2641
Bsh1236I	CGCG	1 cut(s)	2679
BshFI	GGCC	5 cut(s)	67, 130, 777, 1295, 1880
BshNI	GGYRCC	2 cut(s)	2477, 2636
BshTI	ACCGGT	1 cut(s)	479
BsiHKAI	GWGCWC	3 cut(s)	375, 1054, 1897
BsiHKCI	CYCGRG	1 cut(s)	2427
BsiSI	CCGG	3 cut(s)	136, 147, 480
BslFI	GGGAC	3 cut(s)	356, 749, 1888
BslI	CCNNNNNNNGG	3 cut(s)	125, 486, 692
BsmAI	GTCTC	6 cut(s)	610, 706, 1199, 1637, 1844, 2499
BsmFI	GGGAC	3 cut(s)	356, 749, 1888
BsmI	GAATGC	2 cut(s)	2542, 2617
BsnI	GGCC	5 cut(s)	67, 130, 777, 1295, 1880
BsoBI	CYCGRG	1 cut(s)	2427
Bsp119I	TTCGAA	2 cut(s)	940, 2293
Bsp1286I	GDGCHC	5 cut(s)	375, 1054, 1897, 2641, 2723
Bsp1407I	TGTACA	1 cut(s)	2530
Bsp143I	GATC	7 cut(s)	649, 964, 1401, 1737, 2001, 2278, 2625
BspANI	GGCC	5 cut(s)	67, 130, 777, 1295, 1880
BspCNI	CTCAG	5 cut(s)	716, 1418, 1525, 1714, 2140
BspFNI	CGCG	1 cut(s)	2679
BspLI	GGNNCC	7 cut(s)	151, 737, 930, 1879, 2308, 2479, 2638
BspOI	GCTAGC	2 cut(s)	720, 1215
BspPI	GGATC	4 cut(s)	644, 972, 1409, 2273
BspQI	GCTCTTC	1 cut(s)	1488
BspT104I	TTCGAA	2 cut(s)	940, 2293
BspT107I	GGYRCC	2 cut(s)	2477, 2636
BspTI	CTTAAG	1 cut(s)	1045
BsrDI	GCAATG	3 cut(s)	1326, 2009, 2310
BsrFI	RCCGGY	2 cut(s)	146, 479
BsrGI	TGTACA	1 cut(s)	2530
BsrI	ACTGG	7 cut(s)	160, 178, 697, 1059, 1100, 1440, 2491
BssAI	RCCGGY	2 cut(s)	146, 479
BssECI	CCNNGG	1 cut(s)	710
BssMI	GATC	7 cut(s)	649, 964, 1401, 1737, 2001, 2278, 2625
BssNI	GRCGYC	2 cut(s)	8, 2550
BssSI	CACGAG	1 cut(s)	534
Bst2BI	CACGAG	1 cut(s)	534
Bst2UI	CCWGG	3 cut(s)	711, 1297, 2216
Bst4CI	ACNGT	4 cut(s)	290, 460, 1817, 2273
Bst6I	CTCTTC	6 cut(s)	211, 862, 1488, 1947, 2295, 2502
BstACI	GRCGYC	2 cut(s)	8, 2550
BstAFI	CTTAAG	1 cut(s)	1045
BstAPI	GCANNNNNTGC	3 cut(s)	2069, 2180, 2423
BstAUI	TGTACA	1 cut(s)	2530
BstBAI	YACGTR	1 cut(s)	248
BstBI	TTCGAA	2 cut(s)	940, 2293
BstC8I	GCNNGC	5 cut(s)	120, 718, 950, 1213, 1236
BstF5I	GGATG	8 cut(s)	386, 1184, 1369, 1561, 1574, 2120, 2158, 2401
BstFNI	CGCG	1 cut(s)	2679
BstH2I	RGCGCY	1 cut(s)	171
BstHHI	GCGC	4 cut(s)	170, 984, 2516, 2599
BstKTI	GATC	7 cut(s)	652, 967, 1404, 1740, 2004, 2281, 2628
BstMAI	GTCTC	6 cut(s)	610, 706, 1199, 1637, 1844, 2499
BstMBI	GATC	7 cut(s)	649, 964, 1401, 1737, 2001, 2278, 2625
BstNI	CCWGG	3 cut(s)	711, 1297, 2216
BstNSI	RCATGY	2 cut(s)	1097, 1604
BstSCI	CCNGG	3 cut(s)	709, 1295, 2214
BstSFI	CTRYAG	1 cut(s)	672
BstSLI	GKGCMC	2 cut(s)	1054, 2641
BstUI	CGCG	1 cut(s)	2679
BstV2I	GAAGAC	4 cut(s)	87, 528, 687, 2688
BstX2I	RGATCY	5 cut(s)	964, 1401, 1737, 2001, 2278
BstYI	RGATCY	5 cut(s)	964, 1401, 1737, 2001, 2278
BsuRI	GGCC	5 cut(s)	67, 130, 777, 1295, 1880
BtrI	CACGTC	1 cut(s)	1193
BtsCI	GGATG	8 cut(s)	386, 1184, 1369, 1561, 1574, 2120, 2158, 2401
BtsI	GCAGTG	1 cut(s)	525
BtsIMutI	CAGTG	5 cut(s)	153, 525, 1052, 1433, 1709
Cac8I	GCNNGC	5 cut(s)	120, 718, 950, 1213, 1236
CaiI	CAGNNNCTG	2 cut(s)	275, 2221
CfoI	GCGC	4 cut(s)	170, 984, 2516, 2599
Cfr10I	RCCGGY	2 cut(s)	146, 479
Cfr13I	GGNCC	6 cut(s)	128, 640, 736, 1181, 1878, 2653
CseI	GACGC	1 cut(s)	2558
Csp6I	GTAC	4 cut(s)	483, 707, 2478, 2531
CspAI	ACCGGT	1 cut(s)	479
CviQI	GTAC	4 cut(s)	483, 707, 2478, 2531
DpnI	GATC	7 cut(s)	651, 966, 1403, 1739, 2003, 2280, 2627
DpnII	GATC	7 cut(s)	649, 964, 1401, 1737, 2001, 2278, 2625
DrdI	GACNNNNNNGTC	2 cut(s)	1815, 2491
DseDI	GACNNNNNNGTC	2 cut(s)	1815, 2491
EaeI	YGGCCR	1 cut(s)	1293
Eam1104I	CTCTTC	6 cut(s)	211, 862, 1488, 1947, 2295, 2502
EarI	CTCTTC	6 cut(s)	211, 862, 1488, 1947, 2295, 2502
Eco147I	AGGCCT	1 cut(s)	67
Eco24I	GRGCYC	1 cut(s)	2723
Eco47I	GGWCC	4 cut(s)	640, 736, 1181, 2653
Eco57I	CTGAAG	1 cut(s)	2097
Eco88I	CYCGRG	1 cut(s)	2427
EcoO109I	RGGNCCY	1 cut(s)	640
EcoRI	GAATTC	1 cut(s)	1274
EcoRII	CCWGG	3 cut(s)	709, 1295, 2214
EcoT22I	ATGCAT	1 cut(s)	1075
EcoT38I	GRGCYC	1 cut(s)	2723
FalI	AAGNNNNNCTT	2 cut(s)	1773, 1805
FaqI	GGGAC	3 cut(s)	356, 749, 1888
FauI	CCCGC	3 cut(s)	8, 827, 1227
FauNDI	CATATG	1 cut(s)	469
FbaI	TGATCA	1 cut(s)	2625
FokI	GGATG	8 cut(s)	373, 1171, 1376, 1561, 1568, 2107, 2165, 2388
FriOI	GRGCYC	1 cut(s)	2723
GlaI	GCGC	4 cut(s)	169, 983, 2515, 2598
GsuI	CTGGAG	5 cut(s)	195, 605, 1457, 1619, 2325
HaeII	RGCGCY	1 cut(s)	171
HaeIII	GGCC	5 cut(s)	67, 130, 777, 1295, 1880
HapII	CCGG	3 cut(s)	136, 147, 480
HgaI	GACGC	1 cut(s)	2558
HhaI	GCGC	4 cut(s)	170, 984, 2516, 2599
Hin1I	GRCGYC	2 cut(s)	8, 2550
Hin6I	GCGC	4 cut(s)	168, 982, 2514, 2597
HinP1I	GCGC	4 cut(s)	168, 982, 2514, 2597
HincII	GTYRAC	1 cut(s)	1859
HindII	GTYRAC	1 cut(s)	1859
HindIII	AAGCTT	1 cut(s)	1745
HpaII	CCGG	3 cut(s)	136, 147, 480
HphI	GGTGA	4 cut(s)	707, 1516, 2285, 2369
Hpy166II	GTNNAC	5 cut(s)	1052, 1091, 1859, 2399, 2675
Hpy8I	GTNNAC	5 cut(s)	1052, 1091, 1859, 2399, 2675
Hpy99I	CGWCG	1 cut(s)	13
HpyCH4III	ACNGT	4 cut(s)	290, 460, 1817, 2273
HpyCH4IV	ACGT	4 cut(s)	247, 397, 1192, 1569
HpySE526I	ACGT	4 cut(s)	247, 397, 1192, 1569
Hsp92I	GRCGYC	2 cut(s)	8, 2550
HspAI	GCGC	4 cut(s)	168, 982, 2514, 2597
KpnI	GGTACC	1 cut(s)	2481
Ksp22I	TGATCA	1 cut(s)	2625
Kzo9I	GATC	7 cut(s)	649, 964, 1401, 1737, 2001, 2278, 2625
LguI	GCTCTTC	1 cut(s)	1488
LmnI	GCTCC	7 cut(s)	155, 176, 934, 1638, 2218, 2306, 2647
LweI	GCATC	7 cut(s)	262, 395, 1420, 1583, 2121, 2376, 2474
MaeII	ACGT	4 cut(s)	247, 397, 1192, 1569
MaeIII	GTNAC	2 cut(s)	446, 1171
MalI	GATC	7 cut(s)	651, 966, 1403, 1739, 2003, 2280, 2627
MboI	GATC	7 cut(s)	649, 964, 1401, 1737, 2001, 2278, 2625
MfeI	CAATTG	2 cut(s)	337, 1694
MflI	RGATCY	5 cut(s)	964, 1401, 1737, 2001, 2278
MhlI	GDGCHC	5 cut(s)	375, 1054, 1897, 2641, 2723
MlsI	TGGCCA	1 cut(s)	1295
MluNI	TGGCCA	1 cut(s)	1295
MlyI	GAGTC	7 cut(s)	596, 1504, 1693, 1906, 1910, 2224, 2465
MmeI	TCCRAC	1 cut(s)	1740
Mox20I	TGGCCA	1 cut(s)	1295
Mph1103I	ATGCAT	1 cut(s)	1075
MroXI	GAANNNNTTC	1 cut(s)	871
MscI	TGGCCA	1 cut(s)	1295
MseI	TTAA	8 cut(s)	1046, 1068, 1566, 1686, 1742, 1935, 2052, 2139
MslI	CAYNNNNRTG	3 cut(s)	1220, 1554, 2406
Msp20I	TGGCCA	1 cut(s)	1295
MspA1I	CMGCKG	3 cut(s)	1234, 1724, 2177
MspCI	CTTAAG	1 cut(s)	1045
MspI	CCGG	3 cut(s)	136, 147, 480
MspR9I	CCNGG	3 cut(s)	711, 1297, 2216
MunI	CAATTG	2 cut(s)	337, 1694
Mva1269I	GAATGC	2 cut(s)	2542, 2617
MvaI	CCWGG	3 cut(s)	711, 1297, 2216
MvnI	CGCG	1 cut(s)	2679
NdeI	CATATG	1 cut(s)	469
NdeII	GATC	7 cut(s)	649, 964, 1401, 1737, 2001, 2278, 2625
NheI	GCTAGC	2 cut(s)	716, 1211
NlaIV	GGNNCC	7 cut(s)	151, 737, 930, 1879, 2308, 2479, 2638
NsiI	ATGCAT	1 cut(s)	1075
NspI	RCATGY	2 cut(s)	1097, 1604
NspV	TTCGAA	2 cut(s)	940, 2293
PaeR7I	CTCGAG	1 cut(s)	2427
PceI	AGGCCT	1 cut(s)	67
PciI	ACATGT	1 cut(s)	1093
PciSI	GCTCTTC	1 cut(s)	1488
PcsI	WCGNNNNNNNCGW	1 cut(s)	2451
PctI	GAATGC	2 cut(s)	2542, 2617
PdmI	GAANNNNTTC	1 cut(s)	871
PfeI	GAWTC	8 cut(s)	593, 812, 872, 1109, 1136, 1475, 1940, 2041
PflMI	CCANNNNNTGG	1 cut(s)	692
PinAI	ACCGGT	1 cut(s)	479
PleI	GAGTC	7 cut(s)	595, 1504, 1693, 1906, 1909, 2224, 2464
PpsI	GAGTC	7 cut(s)	595, 1504, 1693, 1906, 1909, 2224, 2464
Ppu21I	YACGTR	1 cut(s)	248
PpuMI	RGGWCCY	1 cut(s)	640
PscI	ACATGT	1 cut(s)	1093
PshBI	ATTAAT	1 cut(s)	1068
Psp5II	RGGWCCY	1 cut(s)	640
Psp6I	CCWGG	3 cut(s)	709, 1295, 2214
PspGI	CCWGG	3 cut(s)	709, 1295, 2214
PspN4I	GGNNCC	7 cut(s)	151, 737, 930, 1879, 2308, 2479, 2638
PspPI	GGNCC	6 cut(s)	128, 640, 736, 1181, 1878, 2653
PspPPI	RGGWCCY	1 cut(s)	640
PspXI	VCTCGAGB	1 cut(s)	2427
PstNI	CAGNNNCTG	2 cut(s)	275, 2221
PsuI	RGATCY	5 cut(s)	964, 1401, 1737, 2001, 2278
PvuII	CAGCTG	2 cut(s)	1724, 2177
RsaI	GTAC	4 cut(s)	484, 708, 2479, 2532
RsaNI	GTAC	4 cut(s)	483, 707, 2478, 2531
RseI	CAYNNNNRTG	3 cut(s)	1220, 1554, 2406
SapI	GCTCTTC	1 cut(s)	1488
SaqAI	TTAA	8 cut(s)	1046, 1068, 1566, 1686, 1742, 1935, 2052, 2139
Sau3AI	GATC	7 cut(s)	649, 964, 1401, 1737, 2001, 2278, 2625
Sau96I	GGNCC	6 cut(s)	128, 640, 736, 1181, 1878, 2653
SchI	GAGTC	7 cut(s)	596, 1504, 1693, 1906, 1910, 2224, 2465
ScrFI	CCNGG	3 cut(s)	711, 1297, 2216
SduI	GDGCHC	5 cut(s)	375, 1054, 1897, 2641, 2723
SfaNI	GCATC	7 cut(s)	262, 395, 1420, 1583, 2121, 2376, 2474
SfcI	CTRYAG	1 cut(s)	672
Sfr274I	CTCGAG	1 cut(s)	2427
SfuI	TTCGAA	2 cut(s)	940, 2293
SinI	GGWCC	4 cut(s)	640, 736, 1181, 2653
SlaI	CTCGAG	1 cut(s)	2427
SmiMI	CAYNNNNRTG	3 cut(s)	1220, 1554, 2406
SmlI	CTYRAG	5 cut(s)	1045, 1898, 1914, 2232, 2427
SmoI	CTYRAG	5 cut(s)	1045, 1898, 1914, 2232, 2427
SpeI	ACTAGT	1 cut(s)	1165
SseBI	AGGCCT	1 cut(s)	67
StuI	AGGCCT	1 cut(s)	67
StyD4I	CCNGG	3 cut(s)	709, 1295, 2214
TaaI	ACNGT	4 cut(s)	290, 460, 1817, 2273
TaiI	ACGT	4 cut(s)	250, 400, 1195, 1572
TatI	WGTACW	1 cut(s)	2530
TauI	GCSGC	4 cut(s)	8, 162, 2555, 2749
TfiI	GAWTC	8 cut(s)	593, 812, 872, 1109, 1136, 1475, 1940, 2041
Tru1I	TTAA	8 cut(s)	1046, 1068, 1566, 1686, 1742, 1935, 2052, 2139
Tru9I	TTAA	8 cut(s)	1046, 1068, 1566, 1686, 1742, 1935, 2052, 2139
TscAI	CASTG	5 cut(s)	160, 532, 1059, 1440, 1709
TspRI	CASTG	5 cut(s)	160, 532, 1059, 1440, 1709
Van91I	CCANNNNNTGG	1 cut(s)	692
Vha464I	CTTAAG	1 cut(s)	1045
VneI	GTGCAC	1 cut(s)	1050
VpaK11BI	GGWCC	4 cut(s)	640, 736, 1181, 2653
VspI	ATTAAT	1 cut(s)	1068
XapI	RAATTY	3 cut(s)	79, 1274, 1729
XbaI	TCTAGA	2 cut(s)	2459, 2663
XceI	RCATGY	2 cut(s)	1097, 1604
XhoI	CTCGAG	1 cut(s)	2427
XmnI	GAANNNNTTC	1 cut(s)	871
Zsp2I	ATGCAT	1 cut(s)	1075

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.