Basic Information

Type: gene

Biological Identity

Species

rosa_roxburghii

Chromosome

GWHEROQ00000003

Orthogroup ID

OG0009832

Physical Location & Seq

Genomic Coordinates Reverse (-)

50397162 .. 50407538

Gene Structure 10377 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

Rroxscaffold_3G00255950.1

Sequence Viewer

CDS Protein Promoter (2k)

Length: 2898 bp

ATGGCCGCTTCAGGCGGGTATAATTACCGAGATGCCACTACTCAGAGAAGCTCTTTCAAGGTGGATAGGCCTCTTTCTGTCAATTCCAACCCCAAGTCTGCTGTAAAGTCCAAGCCTTTGCTTCCTCCTCGCCGGAACAGCACTGGCTCTATTGGTGGTGGTGCCGCTGGAGCTGCCTCTAAGGATAATATTGGAGTTCCTGGAAGAGTCCGAGTAGCTGTGAGATTGCGGCCACGTAACTCTGATGAAATGATCTCAGATGCTGATTTTGCTGATATTGTAGAATTACAGCCGGAACTTAAAAGGTTGAAACTTAGGAGAAACAATTGGGACTTGGATACTTATGAGTTCGATGAAGTTCTCACAGAGTTTGCATCACAAAAGCGTGTTTATGAAGTAGTAGCAAAGCCTGTTGTGGAGAGTGTTCTGGAAGGTTATAATGGGACAGTCATGGCATATGGCCAGACTGGTACAGGAAAGACGTATACACTTGGACGACTTGGAGAGGAGGATACTGCTGCTCGTGGAATAATGGTCCGTGCCATGGAGGATATTTTAGCAGATGTATCTCTGGAGTCTGATTCTTTGTCAGTCTCGTATCTGCAGCTTTACATGGAAACCATACATGACCTACTTGATCCTGGAAATGATAGCATTTCTATAGTGGAAGACCCCAAAACTGGTGATGTCTCACTCCCAGGGGCAAGCCTAGTTGAAATCAGGGATCACAAGAGTTTTGTGGAACTACTAAGATTAGGGGAAGCTCACCGCTTTGCTACCAATACAAAACTGAATACTGAGTCTTCTCGTAGCCATGCTATTCTGATGGTACACGTAAAAAGGTCCGTAAAGGGAAGAGATTCAGCTCTTTCAAGCCAAAATGTCAACAACTCCCACACTGTTAAAAGTCTGAAGCCTCCTGTTGTTCGAAAGAGCAAGTTAATTGTGGTGGATCTTGCTGGTTCAGAGCGTATCGACAAATCAGGAAGTGAAGGACATACACTAGAGGAAGCCAAATCAATCAATCTCTCATTAAGTGCACTGGGAAAGTGCATCAATGCATTGGCAGAGAACAGTGCACATATTCCTGTTCGAGATTCAAAACTTACAAGATTGCTTCGGGATTCATTTGGAGGCACAGCAAGAACTTCACTGGTTGTTACTATCGGTCCATCTCCACGTCATCGAGGAGAGACGGCTAGTACTATTATGTTTGGACAGCGGGCTATGAAAGTGGAAAATATGTTGAAATTGAAGGAGGAATTTGACTACAAAAGTTTGTCTAGAAGGCTAGATATACAATTAGACAAACTCATTGCAGAACATGAAAGACAGCAGAAAGCATTTGAGGATGAGATTGAAAGAATAGCCACGGAAGCACAAAATCAGATCTCAGAGAAGGAAAGATTGAAATATCAGAAAGACTATATGGAATCAATAAAAAAGCTTGAAGAGCAGTTGGTGATGAATCAGCCAAAGCATGACTTTGAAAAAGTCACAAATGGACCCAAGGACGATAAATCTAATGTGACATCGAGCAAAGAGAAGTCTGGGAAGAAAAACTGCAGTAGAAAGAATAATCAACTTGGTGCTTTATATATGGAAGTAACATATCATCATGCATTTTATCAAGGTCAAAAGTTAAATGCTATGGATTGGTTGCATGTTGAGGAAATTGATGACTTAAGAAAGTTGCTCCAGCGAGAGACAAGTCTTAGGAAGGCTGCTGAAGAGGAAACTAGTAATCTGAAGAGTCAACTGACTCAATGGAAAAGGTCAGAGGCAGCTGGAAATTCAGAGATCTTAAAGCTTCGTAAGATGCTGGAAGATGAGGCACATCAGAAGGAGAAACTTGAAGGAGAAATAGCAACACTTCACAGTCAATTGTTGCAACTGAGCTTTGATGCTGATGAGAAAGGTAATGAGGCCATATCTGTGGACAATCTGAATAGTGTTGTAATGGCACAGACAAGTAGACAACTTGATAGGAGTGGGCTTGGAAAAGTTGGTGGCCTTGACTCTCTCATGACTCAAGTTAGGCATCTACAGCTCAAGGAATCAGGAAATGAAGATAAAGCCTCAATAGCAAAAATTTTCGAACAAGGTTGCAGTTGCTCTATGGGATTGCAAAAGATCTTGTCATTACTTGAAGCTGAAGACAATGATGTGCGAATTCATGCCGTTAAAGTTGTAGCAAATCTTGCTGCTGAAGAAGCAAATCAGGAGAAAATTGTACAAGCTGGTGGTCTCACATCCTTGTTGATGTTACTCAAAACCTCTGACGATGAAACCATACATAGAGTTGCGGCAGGTGCCATTGCAAATTTAGCAATGAATGAAACCAACCAGGAGCTGATAATGAATCAAGGGGGAATTAGTTTGTTATCTATGACAGCAGCCAACGGTGAAGATCCACAAACCCTTCGAATGGTTGCTGGAGCCATTGCTAATCTTTGCGGAAATGATAAATTACAGAGCAAGCTAAGGGCTGAAGGTGGAATTAAGGCATTGCTAGGAATGGTCAGATGTGGGCATCCAGATGTTCTTGCACAAGTTGCTCGTGGGATTGCAAATTTCGCAAAATGCGAGTCTAGGGCATCTACACAAGGGACCAAAAATGGAAGATCTCTTTTGATCGAGGACGGTGCACTTTCATGGATTGTACAGAATGCTAATAATGAAGCCTCACCAATCAGGCGCCACATTGAGCTAGCATTATGCCACTTAGCGCAACATGAAACAAATGCAAAAGACATGATCAGTGGTGGTGCCTTGTGGGAGCTTGTTCGCATTTCCCGAGACTGTTCACGCGAAGACATAAGAACTCTTGCATATCGAACACTAACTTCTAGCCCCACCTTCCAAGGTGAACTGAGGCGGCTGCGGATAGATAATTAA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0003774 GO:0003777 GO:0003824 GO:0005575 GO:0005622 GO:0005623 GO:0005856 GO:0005871 GO:0005875 GO:0006928 GO:0007017 GO:0007018 GO:0008150 GO:0009987 GO:0015630 GO:0016462 GO:0016787 GO:0016817 GO:0016818 GO:0016887 GO:0017111 GO:0032991 GO:0043226 GO:0043228 GO:0043229 GO:0043232 GO:0044422 GO:0044424 GO:0044430 GO:0044446 GO:0044464

KEGG Pathways

Metabolic & Signaling

ko04144 ko04728 map04144 map04728

Pfam Domains

Protein Families

Arm Kinesin

Protein Analysis

965

Amino Acids

106.55

Weight (kDa)

6.94

Isoelectric Point (pI)

36.87

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Microtub_bd	PF16796	68 - 212	5.6e-15	Microtubule binding
Kinesin	PF00225	75 - 412	1.6e-93	Kinesin motor domain
ARM_ARMC5	PF24768	730 - 934	3.1e-15	ARMC5
Arm	PF00514	740 - 777	9.8e-07	Armadillo/beta-catenin-like repeat

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0009832)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT1G12430 AT1G12430
fragaria_vesca	FvH4_5g00930 FvH4_5g00930 FvH4_5g00930
malus_domestica	MD04G1077000.v1.1 MD06G1067900.v1.1
prunus_persica	Prupe.5G082300_v2.0.a1 Prupe.5G082300_v2.0.a1
pyrus_communis	pycom04g06770
rosa_chinensis	RchiOBHm_Chr7g0200461
rosa_laevigata	RLG00000003701
rosa_multiflora	Rmu_sc0000617.1_g000027 Rmu_sc0002613.1_g000001
rosa_roxburghii	Rroxscaffold_3G00255950
rosa_rugosa	Rorug07G0060400
rosa_samantha	Rh7AG185900 Rh7BG189000 Rh7CG196200 Rh7DG190000
rosa_wichuraiana	Rw7G016300

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	1 cut(s)	438
AarI	CACCTGC	1 cut(s)	2300
Acc36I	ACCTGC	1 cut(s)	2300
AccB1I	GGYRCC	4 cut(s)	161, 2312, 2697, 2768
AccI	GTMKAC	2 cut(s)	485, 1975
AccII	CGCG	1 cut(s)	2811
AclWI	GGATC	4 cut(s)	632, 732, 960, 2405
AcoI	YGGCCR	3 cut(s)	3, 230, 460
AcsI	RAATTY	6 cut(s)	1262, 1792, 2091, 2172, 2323, 2572
AcuI	CTGAAG	6 cut(s)	932, 1749, 1769, 2175, 2229, 2511
AcyI	GRCGYC	1 cut(s)	2698
AfaI	GTAC	5 cut(s)	472, 831, 1204, 2235, 2664
AfiI	CCNNNNNNNGG	3 cut(s)	544, 680, 2428
AflII	CTTAAG	1 cut(s)	1684
AflIII	ACRYGT	1 cut(s)	832
AhlI	ACTAGT	1 cut(s)	1739
AjiI	CACGTC	1 cut(s)	1181
AjnI	CCWGG	4 cut(s)	199, 640, 697, 2346
Alw21I	GWGCWC	3 cut(s)	1042, 1081, 2650
Alw26I	GTCTC	6 cut(s)	598, 694, 1187, 1700, 2252, 2793
Alw44I	GTGCAC	3 cut(s)	1038, 1077, 2646
AlwI	GGATC	4 cut(s)	632, 732, 960, 2405
AlwNI	CAGNNNCTG	2 cut(s)	263, 2353
Ama87I	CYCGRG	1 cut(s)	2796
AoxI	GGCC	6 cut(s)	3, 68, 230, 460, 1926, 2011
ApaLI	GTGCAC	3 cut(s)	1038, 1077, 2646
ApeKI	GCWGC	8 cut(s)	173, 518, 604, 1724, 1784, 2204, 2396, 2881
ApoI	RAATTY	6 cut(s)	1262, 1792, 2091, 2172, 2323, 2572
ArsI	GACNNNNNNTTYG	2 cut(s)	2573, 2605
Asp700I	GAANNNNTTC	1 cut(s)	859
AspLEI	GCGC	2 cut(s)	2700, 2731
AspS9I	GGNCC	5 cut(s)	535, 843, 1169, 1505, 2610
AsuHPI	GGTGA	6 cut(s)	695, 758, 1474, 2417, 2679, 2879
AsuII	TTCGAA	3 cut(s)	928, 2097, 2425
AsuNHI	GCTAGC	1 cut(s)	2710
AvaI	CYCGRG	1 cut(s)	2796
AvaII	GGWCC	5 cut(s)	535, 843, 1169, 1505, 2610
BaeGI	GKGCMC	3 cut(s)	1042, 1081, 2650
BalI	TGGCCA	1 cut(s)	462
BanI	GGYRCC	4 cut(s)	161, 2312, 2697, 2768
BauI	CACGAG	2 cut(s)	522, 2559
BbsI	GAAGAC	4 cut(s)	675, 795, 2163, 2820
Bbv12I	GWGCWC	3 cut(s)	1042, 1081, 2650
BbvI	GCAGC	8 cut(s)	160, 505, 616, 1711, 1796, 2191, 2408, 2868
BccI	CCATC	2 cut(s)	820, 1180
BceAI	ACGGC	2 cut(s)	1212, 2165
BcgI	CGANNNNNNTGC	2 cut(s)	2627, 2661
BciT130I	CCWGG	4 cut(s)	201, 642, 699, 2348
BciVI	GTATCC	2 cut(s)	331, 505
BclI	TGATCA	1 cut(s)	2757
BcoDI	GTCTC	6 cut(s)	598, 694, 1187, 1700, 2252, 2793
BcuI	ACTAGT	1 cut(s)	1739
BfmI	CTRYAG	4 cut(s)	602, 660, 1564, 2045
BfoI	RGCGCY	1 cut(s)	2701
BfrI	CTTAAG	1 cut(s)	1684
BfuAI	ACCTGC	1 cut(s)	2300
BfuI	GTATCC	2 cut(s)	331, 505
BglII	AGATCT	4 cut(s)	1389, 1800, 2133, 2624
BmcAI	AGTACT	1 cut(s)	1204
Bme1390I	CCNGG	4 cut(s)	201, 642, 699, 2348
Bme18I	GGWCC	5 cut(s)	535, 843, 1169, 1505, 2610
BmeT110I	CYCGRG	1 cut(s)	2796
BmgBI	CACGTC	1 cut(s)	1181
BmgT120I	GGNCC	5 cut(s)	535, 843, 1169, 1505, 2610
BmiI	GGNNCC	7 cut(s)	163, 1507, 2314, 2440, 2611, 2699, 2770
BmrFI	CCNGG	4 cut(s)	201, 642, 699, 2348
BmrI	ACTGGG	1 cut(s)	1052
BmsI	GCATC	9 cut(s)	22, 250, 383, 1062, 1809, 1894, 2050, 2542, 2606
BmtI	GCTAGC	1 cut(s)	2714
BmuI	ACTGGG	1 cut(s)	1052
BpiI	GAAGAC	4 cut(s)	675, 795, 2163, 2820
BpmI	CTGGAG	4 cut(s)	189, 593, 1682, 2457
Bpu10I	CCTNAGC	1 cut(s)	2483
Bpu14I	TTCGAA	3 cut(s)	928, 2097, 2425
BpuEI	CTTGAG	2 cut(s)	2016, 2036
BsaAI	YACGTR	2 cut(s)	236, 835
BsaHI	GRCGYC	1 cut(s)	2698
BsaI	GGTCTC	1 cut(s)	2252
BsaJI	CCNNGG	6 cut(s)	543, 697, 698, 1371, 1509, 2863
Bsc4I	CCNNNNNNNGG	3 cut(s)	544, 680, 2428
Bse1I	ACTGG	5 cut(s)	148, 472, 685, 1047, 1158
Bse3DI	GCAATG	5 cut(s)	1314, 2316, 2337, 2442, 2507
BseBI	CCWGG	4 cut(s)	201, 642, 699, 2348
BseDI	CCNNGG	6 cut(s)	543, 697, 698, 1371, 1509, 2863
BseGI	GGATG	3 cut(s)	1357, 2252, 2533
BseLI	CCNNNNNNNGG	3 cut(s)	544, 680, 2428
BseMI	GCAATG	5 cut(s)	1314, 2316, 2337, 2442, 2507
BseMII	CTCAG	6 cut(s)	56, 270, 789, 1407, 1886, 2864
BseNI	ACTGG	5 cut(s)	148, 472, 685, 1047, 1158
BseRI	GAGGAG	3 cut(s)	117, 521, 1203
BseSI	GKGCMC	3 cut(s)	1042, 1081, 2650
BseXI	GCAGC	8 cut(s)	160, 505, 616, 1711, 1796, 2191, 2408, 2868
Bsh1236I	CGCG	1 cut(s)	2811
BshFI	GGCC	6 cut(s)	5, 70, 232, 462, 1928, 2013
BshNI	GGYRCC	4 cut(s)	161, 2312, 2697, 2768
BsiHKAI	GWGCWC	3 cut(s)	1042, 1081, 2650
BsiHKCI	CYCGRG	1 cut(s)	2796
BsiSI	CCGG	2 cut(s)	133, 293
BslFI	GGGAC	3 cut(s)	344, 457, 2623
BslI	CCNNNNNNNGG	3 cut(s)	544, 680, 2428
BsmAI	GTCTC	6 cut(s)	598, 694, 1187, 1700, 2252, 2793
BsmBI	CGTCTC	1 cut(s)	1187
BsmFI	GGGAC	3 cut(s)	344, 457, 2623
BsmI	GAATGC	1 cut(s)	2674
BsnI	GGCC	6 cut(s)	5, 70, 232, 462, 1928, 2013
Bso31I	GGTCTC	1 cut(s)	2252
BsoBI	CYCGRG	1 cut(s)	2796
Bsp119I	TTCGAA	3 cut(s)	928, 2097, 2425
Bsp1286I	GDGCHC	3 cut(s)	1042, 1081, 2650
Bsp1407I	TGTACA	2 cut(s)	2233, 2662
Bsp19I	CCATGG	1 cut(s)	543
BspANI	GGCC	6 cut(s)	5, 70, 232, 462, 1928, 2013
BspCNI	CTCAG	6 cut(s)	55, 269, 790, 1406, 1887, 2865
BspFNI	CGCG	1 cut(s)	2811
BspHI	TCATGA	1 cut(s)	2025
BspLI	GGNNCC	7 cut(s)	163, 1507, 2314, 2440, 2611, 2699, 2770
BspMAI	CTGCAG	2 cut(s)	606, 1568
BspMI	ACCTGC	1 cut(s)	2300
BspOI	GCTAGC	1 cut(s)	2714
BspPI	GGATC	4 cut(s)	632, 732, 960, 2405
BspQI	GCTCTTC	1 cut(s)	1446
BspT104I	TTCGAA	3 cut(s)	928, 2097, 2425
BspT107I	GGYRCC	4 cut(s)	161, 2312, 2697, 2768
BspTI	CTTAAG	1 cut(s)	1684
BspTNI	GGTCTC	1 cut(s)	2252
BsrDI	GCAATG	5 cut(s)	1314, 2316, 2337, 2442, 2507
BsrGI	TGTACA	2 cut(s)	2233, 2662
BsrI	ACTGG	5 cut(s)	148, 472, 685, 1047, 1158
BssECI	CCNNGG	6 cut(s)	543, 697, 698, 1371, 1509, 2863
BssNAI	GTATAC	1 cut(s)	486
BssNI	GRCGYC	1 cut(s)	2698
BssSI	CACGAG	2 cut(s)	522, 2559
BssT1I	CCWWGG	3 cut(s)	543, 1509, 2863
Bst1107I	GTATAC	1 cut(s)	486
Bst2BI	CACGAG	2 cut(s)	522, 2559
Bst2UI	CCWGG	4 cut(s)	201, 642, 699, 2348
Bst4CI	ACNGT	7 cut(s)	448, 901, 1076, 1880, 2405, 2645, 2804
Bst6I	CTCTTC	5 cut(s)	199, 850, 1446, 1725, 1745
BstACI	GRCGYC	1 cut(s)	2698
BstAFI	CTTAAG	1 cut(s)	1684
BstAPI	GCANNNNNTGC	2 cut(s)	2201, 2555
BstAUI	TGTACA	2 cut(s)	2233, 2662
BstBAI	YACGTR	2 cut(s)	236, 835
BstBI	TTCGAA	3 cut(s)	928, 2097, 2425
BstC8I	GCNNGC	4 cut(s)	706, 1224, 2480, 2712
BstDSI	CCRYGG	2 cut(s)	543, 1371
BstF5I	GGATG	3 cut(s)	1357, 2252, 2533
BstFNI	CGCG	1 cut(s)	2811
BstH2I	RGCGCY	1 cut(s)	2701
BstHHI	GCGC	2 cut(s)	2700, 2731
BstMAI	GTCTC	6 cut(s)	598, 694, 1187, 1700, 2252, 2793
BstNI	CCWGG	4 cut(s)	201, 642, 699, 2348
BstNSI	RCATGY	1 cut(s)	1667
BstSCI	CCNGG	4 cut(s)	199, 640, 697, 2346
BstSFI	CTRYAG	4 cut(s)	602, 660, 1564, 2045
BstSLI	GKGCMC	3 cut(s)	1042, 1081, 2650
BstUI	CGCG	1 cut(s)	2811
BstV1I	GCAGC	8 cut(s)	160, 505, 616, 1711, 1796, 2191, 2408, 2868
BstV2I	GAAGAC	4 cut(s)	675, 795, 2163, 2820
BstX2I	RGATCY	6 cut(s)	952, 1389, 1800, 2133, 2410, 2624
BstXI	CCANNNNNNTGG	1 cut(s)	1936
BstYI	RGATCY	6 cut(s)	952, 1389, 1800, 2133, 2410, 2624
BstZ17I	GTATAC	1 cut(s)	486
BsuI	GTATCC	2 cut(s)	331, 505
BsuRI	GGCC	6 cut(s)	5, 70, 232, 462, 1928, 2013
BtgI	CCRYGG	2 cut(s)	543, 1371
BtrI	CACGTC	1 cut(s)	1181
BtsCI	GGATG	3 cut(s)	1357, 2252, 2533
BtsIMutI	CAGTG	6 cut(s)	141, 897, 1040, 1081, 1151, 2767
BveI	ACCTGC	1 cut(s)	2300
Cac8I	GCNNGC	4 cut(s)	706, 1224, 2480, 2712
CaiI	CAGNNNCTG	2 cut(s)	263, 2353
CciI	TCATGA	1 cut(s)	2025
CfoI	GCGC	2 cut(s)	2700, 2731
Cfr13I	GGNCC	5 cut(s)	535, 843, 1169, 1505, 2610
Csp6I	GTAC	5 cut(s)	471, 830, 1203, 2234, 2663
CviQI	GTAC	5 cut(s)	471, 830, 1203, 2234, 2663
DinI	GGCGCC	1 cut(s)	2699
EaeI	YGGCCR	3 cut(s)	3, 230, 460
Eam1104I	CTCTTC	5 cut(s)	199, 850, 1446, 1725, 1745
EarI	CTCTTC	5 cut(s)	199, 850, 1446, 1725, 1745
Eco130I	CCWWGG	3 cut(s)	543, 1509, 2863
Eco147I	AGGCCT	1 cut(s)	70
Eco31I	GGTCTC	1 cut(s)	2252
Eco47I	GGWCC	5 cut(s)	535, 843, 1169, 1505, 2610
Eco57I	CTGAAG	6 cut(s)	932, 1749, 1769, 2175, 2229, 2511
Eco88I	CYCGRG	1 cut(s)	2796
EcoRI	GAATTC	1 cut(s)	2172
EcoRII	CCWGG	4 cut(s)	199, 640, 697, 2346
EcoT14I	CCWWGG	3 cut(s)	543, 1509, 2863
EcoT22I	ATGCAT	2 cut(s)	1063, 1624
EgeI	GGCGCC	1 cut(s)	2699
EheI	GGCGCC	1 cut(s)	2699
ErhI	CCWWGG	3 cut(s)	543, 1509, 2863
Esp3I	CGTCTC	1 cut(s)	1187
FalI	AAGNNNNNCTT	2 cut(s)	1469, 1501
FaqI	GGGAC	3 cut(s)	344, 457, 2623
FauI	CCCGC	2 cut(s)	8, 1215
FauNDI	CATATG	1 cut(s)	457
FbaI	TGATCA	1 cut(s)	2757
FblI	GTMKAC	2 cut(s)	485, 1975
FokI	GGATG	3 cut(s)	1364, 2239, 2520
GlaI	GCGC	2 cut(s)	2699, 2730
GsuI	CTGGAG	4 cut(s)	189, 593, 1682, 2457
HaeII	RGCGCY	1 cut(s)	2701
HaeIII	GGCC	6 cut(s)	5, 70, 232, 462, 1928, 2013
HapII	CCGG	2 cut(s)	133, 293
HhaI	GCGC	2 cut(s)	2700, 2731
Hin1I	GRCGYC	1 cut(s)	2698
Hin6I	GCGC	2 cut(s)	2698, 2729
HinP1I	GCGC	2 cut(s)	2698, 2729
HincII	GTYRAC	2 cut(s)	886, 1757
HindII	GTYRAC	2 cut(s)	886, 1757
HindIII	AAGCTT	2 cut(s)	1445, 1808
HpaII	CCGG	2 cut(s)	133, 293
HphI	GGTGA	6 cut(s)	695, 758, 1474, 2417, 2679, 2879
HpyCH4III	ACNGT	7 cut(s)	448, 901, 1076, 1880, 2405, 2645, 2804
HpyCH4IV	ACGT	4 cut(s)	235, 482, 834, 1180
HpySE526I	ACGT	4 cut(s)	235, 482, 834, 1180
Hsp92I	GRCGYC	1 cut(s)	2698
HspAI	GCGC	2 cut(s)	2698, 2729
KasI	GGCGCC	1 cut(s)	2697
Ksp22I	TGATCA	1 cut(s)	2757
LguI	GCTCTTC	1 cut(s)	1446
LmnI	GCTCC	5 cut(s)	170, 1701, 2350, 2438, 2779
Lsp1109I	GCAGC	8 cut(s)	160, 505, 616, 1711, 1796, 2191, 2408, 2868
LweI	GCATC	9 cut(s)	22, 250, 383, 1062, 1809, 1894, 2050, 2542, 2606
MaeII	ACGT	4 cut(s)	235, 482, 834, 1180
MaeIII	GTNAC	6 cut(s)	236, 1159, 1495, 1528, 1606, 2265
MfeI	CAATTG	2 cut(s)	325, 1883
MflI	RGATCY	6 cut(s)	952, 1389, 1800, 2133, 2410, 2624
MhlI	GDGCHC	3 cut(s)	1042, 1081, 2650
MlsI	TGGCCA	1 cut(s)	462
MluNI	TGGCCA	1 cut(s)	462
Mly113I	GGCGCC	1 cut(s)	2698
MlyI	GAGTC	8 cut(s)	216, 584, 809, 1756, 1762, 2012, 2023, 2597
MmeI	TCCRAC	1 cut(s)	111
Mox20I	TGGCCA	1 cut(s)	462
Mph1103I	ATGCAT	2 cut(s)	1063, 1624
MroXI	GAANNNNTTC	1 cut(s)	859
MscI	TGGCCA	1 cut(s)	462
MslI	CAYNNNNRTG	3 cut(s)	1934, 2538, 2653
Msp20I	TGGCCA	1 cut(s)	462
MspA1I	CMGCKG	3 cut(s)	167, 1222, 1787
MspCI	CTTAAG	1 cut(s)	1684
MspI	CCGG	2 cut(s)	133, 293
MspR9I	CCNGG	4 cut(s)	201, 642, 699, 2348
MunI	CAATTG	2 cut(s)	325, 1883
Mva1269I	GAATGC	1 cut(s)	2674
MvaI	CCWGG	4 cut(s)	201, 642, 699, 2348
MvnI	CGCG	1 cut(s)	2811
NarI	GGCGCC	1 cut(s)	2698
NcoI	CCATGG	1 cut(s)	543
NdeI	CATATG	1 cut(s)	457
NheI	GCTAGC	1 cut(s)	2710
NlaIV	GGNNCC	7 cut(s)	163, 1507, 2314, 2440, 2611, 2699, 2770
NmuCI	GTSAC	2 cut(s)	1495, 1528
NsiI	ATGCAT	2 cut(s)	1063, 1624
NspI	RCATGY	1 cut(s)	1667
NspV	TTCGAA	3 cut(s)	928, 2097, 2425
PagI	TCATGA	1 cut(s)	2025
PaqCI	CACCTGC	1 cut(s)	2300
PasI	CCCWGGG	1 cut(s)	698
PceI	AGGCCT	1 cut(s)	70
PciSI	GCTCTTC	1 cut(s)	1446
PcsI	WCGNNNNNNNCGW	2 cut(s)	2583, 2794
PctI	GAATGC	1 cut(s)	2674
PdmI	GAANNNNTTC	1 cut(s)	859
PfeI	GAWTC	8 cut(s)	581, 860, 1097, 1124, 1433, 1468, 2057, 2362
PfoI	TCCNGGA	2 cut(s)	199, 640
PleI	GAGTC	8 cut(s)	215, 583, 808, 1756, 1761, 2012, 2023, 2596
PluTI	GGCGCC	1 cut(s)	2701
PpsI	GAGTC	8 cut(s)	215, 583, 808, 1756, 1761, 2012, 2023, 2596
Ppu21I	YACGTR	2 cut(s)	236, 835
PsiI	TTATAA	1 cut(s)	438
Psp6I	CCWGG	4 cut(s)	199, 640, 697, 2346
PspGI	CCWGG	4 cut(s)	199, 640, 697, 2346
PspN4I	GGNNCC	7 cut(s)	163, 1507, 2314, 2440, 2611, 2699, 2770
PspPI	GGNCC	5 cut(s)	535, 843, 1169, 1505, 2610
PstI	CTGCAG	2 cut(s)	606, 1568
PstNI	CAGNNNCTG	2 cut(s)	263, 2353
PsuI	RGATCY	6 cut(s)	952, 1389, 1800, 2133, 2410, 2624
PvuII	CAGCTG	1 cut(s)	1787
RsaI	GTAC	5 cut(s)	472, 831, 1204, 2235, 2664
RsaNI	GTAC	5 cut(s)	471, 830, 1203, 2234, 2663
RseI	CAYNNNNRTG	3 cut(s)	1934, 2538, 2653
SapI	GCTCTTC	1 cut(s)	1446
Sau96I	GGNCC	5 cut(s)	535, 843, 1169, 1505, 2610
ScaI	AGTACT	1 cut(s)	1204
SchI	GAGTC	8 cut(s)	216, 584, 809, 1756, 1762, 2012, 2023, 2597
ScrFI	CCNGG	4 cut(s)	201, 642, 699, 2348
SduI	GDGCHC	3 cut(s)	1042, 1081, 2650
SfaNI	GCATC	9 cut(s)	22, 250, 383, 1062, 1809, 1894, 2050, 2542, 2606
SfcI	CTRYAG	4 cut(s)	602, 660, 1564, 2045
SfoI	GGCGCC	1 cut(s)	2699
SfuI	TTCGAA	3 cut(s)	928, 2097, 2425
SinI	GGWCC	5 cut(s)	535, 843, 1169, 1505, 2610
SmiMI	CAYNNNNRTG	3 cut(s)	1934, 2538, 2653
SmlI	CTYRAG	3 cut(s)	1684, 2031, 2051
SmoI	CTYRAG	3 cut(s)	1684, 2031, 2051
SpeI	ACTAGT	1 cut(s)	1739
SseBI	AGGCCT	1 cut(s)	70
SspDI	GGCGCC	1 cut(s)	2697
SspI	AATATT	1 cut(s)	190
StuI	AGGCCT	1 cut(s)	70
StyD4I	CCNGG	4 cut(s)	199, 640, 697, 2346
StyI	CCWWGG	3 cut(s)	543, 1509, 2863
TaaI	ACNGT	7 cut(s)	448, 901, 1076, 1880, 2405, 2645, 2804
TaiI	ACGT	4 cut(s)	238, 485, 837, 1183
TaqII	GACCGA	1 cut(s)	1157
TatI	WGTACW	3 cut(s)	1202, 2233, 2662
TauI	GCSGC	5 cut(s)	8, 167, 232, 2309, 2881
TfiI	GAWTC	8 cut(s)	581, 860, 1097, 1124, 1433, 1468, 2057, 2362
TscAI	CASTG	6 cut(s)	148, 904, 1047, 1081, 1158, 2767
TseFI	GTSAC	2 cut(s)	1495, 1528
TseI	GCWGC	8 cut(s)	173, 518, 604, 1724, 1784, 2204, 2396, 2881
Tsp45I	GTSAC	2 cut(s)	1495, 1528
TspGWI	ACGGA	3 cut(s)	527, 835, 1388
TspRI	CASTG	6 cut(s)	148, 904, 1047, 1081, 1158, 2767
Vha464I	CTTAAG	1 cut(s)	1684
VneI	GTGCAC	3 cut(s)	1038, 1077, 2646
VpaK11BI	GGWCC	5 cut(s)	535, 843, 1169, 1505, 2610
XapI	RAATTY	6 cut(s)	1262, 1792, 2091, 2172, 2323, 2572
XbaI	TCTAGA	1 cut(s)	1283
XceI	RCATGY	1 cut(s)	1667
XmiI	GTMKAC	2 cut(s)	485, 1975
XmnI	GAANNNNTTC	1 cut(s)	859
ZrmI	AGTACT	1 cut(s)	1204
Zsp2I	ATGCAT	2 cut(s)	1063, 1624

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.