MD04G1100200.v1.1
ERF Family

Belongs to the protein kinase superfamily. Ser Thr protein kinase family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr04
OG0018322
Physical Location & Seq
Reverse (-)
18514931 .. 18515371
441 bp
Loading structure...
UTR
Exon/CDS
Intron
MD04G1100200.v1.1.491

Sequence Viewer

Length: 441 bp
ATGGGTTGTGTCGGTGTTTTCTTTCCTATCGTCGGGACCCGCGGTTCGTTGCGGCCAGTCATTGACCTCAGCTCAAACTCTCTATTAACCGGGTTGATCCCGCCAGAGCTCGGGAAGCTCATCGACCTTGAAATGCTGACAGCAGGAGGGAACAAGTACATTGGTGAGAAAATCCCGGAAGAGATCGGAGGCTGCTGCAATTTGACTGTCTTAGGGTTGGCAGACACCCAAGTTTCAGGCACTTTACCTGCCTCATTGAGAAAGCTTAGCAATCTCCAAACCCTGTCTATTTACACCACAATGATCTTTGGTGAAATCCCACCTGAAATAGGTAACTGCTTTGAGCTTGTAAGCTTGTTTCTTTACAAAAATAACCTCTCTGGTTCGATCCCATGTGAGCTCGGGAAGCTTAAGAAGCTGCAGCAGTTGCTACTGTGGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
LRRNT_2 LRR_1 LRR_6 LRR_8 Pkinase Pkinase_Tyr

Protein Analysis

147

Amino Acids

15.68

Weight (kDa)

5.41

Isoelectric Point (pI)

27.44

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
LRR_14 PF23598 20 - 143 2.4e-10 Leucine-rich repeat region
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0018322)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G54730
malus_domestica MD04G1100200.v1.1
rosa_chinensis RchiOBHm_Chr3g0463431 RchiOBHm_Chr6g0293031
rosa_laevigata RLG00000014314
rosa_multiflora Rmu_co8197710.1_g000001 Rmu_sc0025580.1_g000001
rosa_rugosa Rorug01G0145000.1
rosa_samantha Rh2AG056800
rosa_wichuraiana Rw5G038540

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 256
AccII CGCG 1 cut(s) 42
AciI CCGC 4 cut(s) 40, 42, 52, 101
AclWI GGATC 2 cut(s) 91, 382
AcoI YGGCCR 1 cut(s) 53
AfaI GTAC 1 cut(s) 158
AfiI CCNNNNNNNGG 3 cut(s) 32, 110, 329
AflII CTTAAG 1 cut(s) 410
AgsI TTSAA 1 cut(s) 131
AloI GAACNNNNNNTCC 2 cut(s) 28, 60
AluBI AGCT 9 cut(s) 72, 109, 118, 265, 346, 354, 400, 409, 418
AluI AGCT 9 cut(s) 72, 109, 118, 265, 346, 354, 400, 409, 418
Alw21I GWGCWC 2 cut(s) 111, 402
AlwI GGATC 2 cut(s) 91, 382
Ama87I CYCGRG 2 cut(s) 110, 401
AoxI GGCC 1 cut(s) 53
ApeKI GCWGC 4 cut(s) 192, 195, 418, 421
ArsI GACNNNNNNTTYG 2 cut(s) 28, 60
AspS9I GGNCC 1 cut(s) 36
AsuC2I CCSGG 2 cut(s) 91, 176
AsuHPI GGTGA 2 cut(s) 176, 323
AvaI CYCGRG 2 cut(s) 110, 401
AvaII GGWCC 1 cut(s) 36
BanII GRGCYC 2 cut(s) 111, 402
Bbv12I GWGCWC 2 cut(s) 111, 402
BbvCI CCTCAGC 1 cut(s) 68
BbvI GCAGC 4 cut(s) 179, 182, 405, 433
BcnI CCSGG 2 cut(s) 91, 176
BfmI CTRYAG 1 cut(s) 419
BfrI CTTAAG 1 cut(s) 410
BfuAI ACCTGC 1 cut(s) 256
BisI GCNGC 5 cut(s) 53, 193, 196, 419, 422
BlpI GCTNAGC 1 cut(s) 266
BlsI GCNGC 5 cut(s) 54, 194, 197, 420, 423
Bme1390I CCNGG 2 cut(s) 91, 176
Bme18I GGWCC 1 cut(s) 36
BmeT110I CYCGRG 2 cut(s) 110, 401
BmgT120I GGNCC 1 cut(s) 36
BmiI GGNNCC 2 cut(s) 37, 38
BmrFI CCNGG 2 cut(s) 91, 176
Bpu10I CCTNAGC 1 cut(s) 68
Bpu1102I GCTNAGC 1 cut(s) 266
BpuMI CCSGG 2 cut(s) 91, 176
BsaJI CCNNGG 1 cut(s) 40
Bsc4I CCNNNNNNNGG 3 cut(s) 32, 110, 329
Bse1I ACTGG 1 cut(s) 56
BseDI CCNNGG 1 cut(s) 40
BseLI CCNNNNNNNGG 3 cut(s) 32, 110, 329
BseMII CTCAG 1 cut(s) 82
BseNI ACTGG 1 cut(s) 56
BseXI GCAGC 4 cut(s) 179, 182, 405, 433
Bsh1236I CGCG 1 cut(s) 42
BshFI GGCC 1 cut(s) 55
BsiHKAI GWGCWC 2 cut(s) 111, 402
BsiHKCI CYCGRG 2 cut(s) 110, 401
BsiSI CCGG 2 cut(s) 90, 176
BslFI GGGAC 1 cut(s) 49
BslI CCNNNNNNNGG 3 cut(s) 32, 110, 329
BsmFI GGGAC 1 cut(s) 49
BsnI GGCC 1 cut(s) 55
BsoBI CYCGRG 2 cut(s) 110, 401
Bsp1286I GDGCHC 2 cut(s) 111, 402
Bsp143I GATC 4 cut(s) 96, 183, 303, 387
Bsp1720I GCTNAGC 1 cut(s) 266
BspACI CCGC 4 cut(s) 40, 42, 52, 101
BspANI GGCC 1 cut(s) 55
BspCNI CTCAG 1 cut(s) 81
BspFNI CGCG 1 cut(s) 42
BspLI GGNNCC 2 cut(s) 37, 38
BspMAI CTGCAG 1 cut(s) 423
BspMI ACCTGC 1 cut(s) 256
BspPI GGATC 2 cut(s) 91, 382
BspTI CTTAAG 1 cut(s) 410
BsrI ACTGG 1 cut(s) 56
BssECI CCNNGG 1 cut(s) 40
BssMI GATC 4 cut(s) 96, 183, 303, 387
Bst4CI ACNGT 2 cut(s) 208, 435
Bst6I CTCTTC 1 cut(s) 174
BstAFI CTTAAG 1 cut(s) 410
BstAPI GCANNNNNTGC 1 cut(s) 427
BstDEI CTNAG 3 cut(s) 68, 211, 266
BstDSI CCRYGG 1 cut(s) 40
BstENI CCTNNNNNAGG 1 cut(s) 327
BstFNI CGCG 1 cut(s) 42
BstKTI GATC 4 cut(s) 99, 186, 306, 390
BstMBI GATC 4 cut(s) 96, 183, 303, 387
BstMWI GCNNNNNNNGC 4 cut(s) 115, 406, 415, 427
BstSCI CCNGG 2 cut(s) 89, 174
BstSFI CTRYAG 1 cut(s) 419
BstUI CGCG 1 cut(s) 42
BstV1I GCAGC 4 cut(s) 179, 182, 405, 433
BsuRI GGCC 1 cut(s) 55
BtgI CCRYGG 1 cut(s) 40
BveI ACCTGC 1 cut(s) 256
Cfr13I GGNCC 1 cut(s) 36
Cfr42I CCGCGG 1 cut(s) 43
Csp6I GTAC 1 cut(s) 157
CviAII CATG 1 cut(s) 393
CviQI GTAC 1 cut(s) 157
DdeI CTNAG 3 cut(s) 68, 211, 266
DpnI GATC 4 cut(s) 98, 185, 305, 389
DpnII GATC 4 cut(s) 96, 183, 303, 387
EaeI YGGCCR 1 cut(s) 53
Eam1104I CTCTTC 1 cut(s) 174
EarI CTCTTC 1 cut(s) 174
Ecl136II GAGCTC 2 cut(s) 109, 400
Eco24I GRGCYC 2 cut(s) 111, 402
Eco47I GGWCC 1 cut(s) 36
Eco53kI GAGCTC 2 cut(s) 109, 400
Eco88I CYCGRG 2 cut(s) 110, 401
EcoICRI GAGCTC 2 cut(s) 109, 400
EcoNI CCTNNNNNAGG 1 cut(s) 327
EcoO109I RGGNCCY 1 cut(s) 36
EcoT38I GRGCYC 2 cut(s) 111, 402
FaeI CATG 1 cut(s) 396
FaiI YATR 1 cut(s) 394
FaqI GGGAC 1 cut(s) 49
FatI CATG 1 cut(s) 392
FauI CCCGC 2 cut(s) 47, 108
Fnu4HI GCNGC 5 cut(s) 53, 193, 196, 419, 422
FriOI GRGCYC 2 cut(s) 111, 402
Fsp4HI GCNGC 5 cut(s) 53, 193, 196, 419, 422
GluI GCNGC 5 cut(s) 53, 193, 196, 419, 422
HaeIII GGCC 1 cut(s) 55
HapII CCGG 2 cut(s) 90, 176
Hin1II CATG 1 cut(s) 396
HindIII AAGCTT 3 cut(s) 263, 352, 407
HpaII CCGG 2 cut(s) 90, 176
HphI GGTGA 2 cut(s) 176, 323
Hpy188I TCNGA 1 cut(s) 188
Hpy188III TCNNGA 3 cut(s) 34, 112, 403
Hpy99I CGWCG 1 cut(s) 35
HpyCH4III ACNGT 2 cut(s) 208, 435
HpyCH4V TGCA 2 cut(s) 198, 421
HpyF10VI GCNNNNNNNGC 4 cut(s) 115, 406, 415, 427
HpyF3I CTNAG 3 cut(s) 68, 211, 266
Hsp92II CATG 1 cut(s) 396
KflI GGGWCCC 1 cut(s) 36
KspI CCGCGG 1 cut(s) 43
Kzo9I GATC 4 cut(s) 96, 183, 303, 387
Lsp1109I GCAGC 4 cut(s) 179, 182, 405, 433
MaeIII GTNAC 1 cut(s) 332
MalI GATC 4 cut(s) 98, 185, 305, 389
MboI GATC 4 cut(s) 96, 183, 303, 387
MboII GAAGA 1 cut(s) 191
MhlI GDGCHC 2 cut(s) 111, 402
MluCI AATT 1 cut(s) 199
MnlI CCTC 5 cut(s) 77, 140, 182, 262, 386
MseI TTAA 2 cut(s) 86, 411
MslI CAYNNNNRTG 1 cut(s) 299
MspA1I CMGCKG 1 cut(s) 42
MspCI CTTAAG 1 cut(s) 410
MspI CCGG 2 cut(s) 90, 176
MspR9I CCNGG 2 cut(s) 91, 176
MvnI CGCG 1 cut(s) 42
MwoI GCNNNNNNNGC 4 cut(s) 115, 406, 415, 427
NciI CCSGG 2 cut(s) 91, 176
NdeII GATC 4 cut(s) 96, 183, 303, 387
NlaIII CATG 1 cut(s) 396
NlaIV GGNNCC 2 cut(s) 37, 38
PfoI TCCNGGA 1 cut(s) 174
PkrI GCNGC 5 cut(s) 54, 194, 197, 420, 423
PpuMI RGGWCCY 1 cut(s) 36
Psp124BI GAGCTC 2 cut(s) 111, 402
Psp5II RGGWCCY 1 cut(s) 36
PspN4I GGNNCC 2 cut(s) 37, 38
PspPI GGNCC 1 cut(s) 36
PspPPI RGGWCCY 1 cut(s) 36
PstI CTGCAG 1 cut(s) 423
RsaI GTAC 1 cut(s) 158
RsaNI GTAC 1 cut(s) 157
RseI CAYNNNNRTG 1 cut(s) 299
SacI GAGCTC 2 cut(s) 111, 402
SacII CCGCGG 1 cut(s) 43
SaqAI TTAA 2 cut(s) 86, 411
SatI GCNGC 5 cut(s) 53, 193, 196, 419, 422
Sau3AI GATC 4 cut(s) 96, 183, 303, 387
Sau96I GGNCC 1 cut(s) 36
ScrFI CCNGG 2 cut(s) 91, 176
SduI GDGCHC 2 cut(s) 111, 402
SfcI CTRYAG 1 cut(s) 419
Sfr303I CCGCGG 1 cut(s) 43
SgrBI CCGCGG 1 cut(s) 43
SinI GGWCC 1 cut(s) 36
SmiMI CAYNNNNRTG 1 cut(s) 299
SmlI CTYRAG 1 cut(s) 410
SmoI CTYRAG 1 cut(s) 410
Sse9I AATT 1 cut(s) 199
SsiI CCGC 4 cut(s) 40, 42, 52, 101
SstI GAGCTC 2 cut(s) 111, 402
StyD4I CCNGG 2 cut(s) 89, 174
TaaI ACNGT 2 cut(s) 208, 435
TaqI TCGA 2 cut(s) 123, 386
TasI AATT 1 cut(s) 199
TatI WGTACW 1 cut(s) 156
TauI GCSGC 1 cut(s) 55
Tru1I TTAA 2 cut(s) 86, 411
Tru9I TTAA 2 cut(s) 86, 411
TseI GCWGC 4 cut(s) 192, 195, 418, 421
Vha464I CTTAAG 1 cut(s) 410
VpaK11BI GGWCC 1 cut(s) 36
XagI CCTNNNNNAGG 1 cut(s) 327
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.