RchiOBHm_Chr3g0463431

LRR receptor-like serine threonine-protein kinase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
3
OG0018322
Physical Location & Seq
Reverse (-)
10771105 .. 10771455
351 bp
Loading structure...
UTR
Exon/CDS
Intron
PRQ42975

Sequence Viewer

Length: 351 bp
ATGTCTCAAAATTCTTTGAGTGTTTCCCTACCGGATGAAGTGGGCACTTTAGTAAATCCTGCTGAGCTAGATGTATCAGGAAACAAGTTATCAGGTGAGATCCCCATTACGTACCTTGGCAGCTGTACTAGCTTAGTTAGCCTGCATTTGGAAGGTAATGAATTCAAAGGACATATTCCTCAATCTCTAGAAAAGTTAAGAGGCTTGGATCAGATGGATATTTCTCGGAATAACTTACCTGGACAGATTCCAGAATTTTTAGGCAAGCTTGGAGCTCTTAGGTATTTCAATCTTTCTCAAAATGATTTTGAAGGTGAATTGCTGACAGGACCCCCCCGGATTTCACCCTGA

Protein Analysis

116

Amino Acids

12.56

Weight (kDa)

4.72

Isoelectric Point (pI)

38.58

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
LRR_8 PF13855 44 - 103 1.8e-07 Leucine rich repeat
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Orthologous Genes (Group: OG0018322)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G54730
malus_domestica MD04G1100200.v1.1
rosa_chinensis RchiOBHm_Chr3g0463431 RchiOBHm_Chr6g0293031
rosa_laevigata RLG00000014314
rosa_multiflora Rmu_co8197710.1_g000001 Rmu_sc0025580.1_g000001
rosa_rugosa Rorug01G0145000.1
rosa_samantha Rh2AG056800
rosa_wichuraiana Rw5G038540

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AclWI GGATC 2 cut(s) 94, 216
AcsI RAATTY 3 cut(s) 10, 161, 254
AfaI GTAC 2 cut(s) 113, 127
AfiI CCNNNNNNNGG 1 cut(s) 148
AgsI TTSAA 3 cut(s) 166, 289, 311
AjnI CCWGG 1 cut(s) 238
AluBI AGCT 5 cut(s) 67, 123, 132, 268, 275
AluI AGCT 5 cut(s) 67, 123, 132, 268, 275
Alw21I GWGCWC 1 cut(s) 277
Alw26I GTCTC 1 cut(s) 9
AlwI GGATC 2 cut(s) 94, 216
ApeKI GCWGC 1 cut(s) 120
ApoI RAATTY 3 cut(s) 10, 161, 254
AspS9I GGNCC 1 cut(s) 329
AsuC2I CCSGG 1 cut(s) 337
AsuHPI GGTGA 3 cut(s) 107, 326, 336
AvaII GGWCC 1 cut(s) 329
BaeGI GKGCMC 1 cut(s) 47
BanII GRGCYC 1 cut(s) 277
Bbv12I GWGCWC 1 cut(s) 277
BbvI GCAGC 1 cut(s) 132
BccI CCATC 1 cut(s) 208
BciT130I CCWGG 1 cut(s) 240
BcnI CCSGG 1 cut(s) 337
BcoDI GTCTC 1 cut(s) 9
BfaI CTAG 3 cut(s) 68, 129, 188
BisI GCNGC 1 cut(s) 121
BlpI GCTNAGC 1 cut(s) 63
BlsI GCNGC 1 cut(s) 122
Bme1390I CCNGG 2 cut(s) 240, 337
Bme18I GGWCC 1 cut(s) 329
BmgT120I GGNCC 1 cut(s) 329
BmiI GGNNCC 1 cut(s) 331
BmrFI CCNGG 2 cut(s) 240, 337
Bpu1102I GCTNAGC 1 cut(s) 63
BpuMI CCSGG 1 cut(s) 337
BsaAI YACGTR 1 cut(s) 111
BsaJI CCNNGG 2 cut(s) 115, 335
BsaWI WCCGGW 1 cut(s) 31
Bsc4I CCNNNNNNNGG 1 cut(s) 148
BseBI CCWGG 1 cut(s) 240
BseDI CCNNGG 2 cut(s) 115, 335
BseGI GGATG 1 cut(s) 40
BseLI CCNNNNNNNGG 1 cut(s) 148
BseMII CTCAG 1 cut(s) 54
BseSI GKGCMC 1 cut(s) 47
BseXI GCAGC 1 cut(s) 132
BsiHKAI GWGCWC 1 cut(s) 277
BsiSI CCGG 2 cut(s) 32, 337
BslI CCNNNNNNNGG 1 cut(s) 148
BsmAI GTCTC 1 cut(s) 9
Bsp1286I GDGCHC 2 cut(s) 47, 277
Bsp143I GATC 2 cut(s) 99, 208
Bsp1720I GCTNAGC 1 cut(s) 63
BspCNI CTCAG 1 cut(s) 55
BspLI GGNNCC 1 cut(s) 331
BspPI GGATC 2 cut(s) 94, 216
BssECI CCNNGG 2 cut(s) 115, 335
BssMI GATC 2 cut(s) 99, 208
BssT1I CCWWGG 1 cut(s) 115
Bst2UI CCWGG 1 cut(s) 240
BstBAI YACGTR 1 cut(s) 111
BstC8I GCNNGC 2 cut(s) 143, 266
BstDEI CTNAG 3 cut(s) 63, 133, 278
BstF5I GGATG 1 cut(s) 40
BstKTI GATC 2 cut(s) 102, 211
BstMAI GTCTC 1 cut(s) 9
BstMBI GATC 2 cut(s) 99, 208
BstMWI GCNNNNNNNGC 2 cut(s) 129, 138
BstNI CCWGG 1 cut(s) 240
BstSCI CCNGG 2 cut(s) 238, 335
BstSLI GKGCMC 1 cut(s) 47
BstSNI TACGTA 1 cut(s) 111
BstV1I GCAGC 1 cut(s) 132
BstX2I RGATCY 1 cut(s) 99
BstYI RGATCY 1 cut(s) 99
BtsCI GGATG 1 cut(s) 40
Cac8I GCNNGC 2 cut(s) 143, 266
Cfr13I GGNCC 1 cut(s) 329
Csp6I GTAC 2 cut(s) 112, 126
CviJI RGCY 7 cut(s) 67, 123, 132, 141, 204, 268, 275
CviKI_1 RGCY 7 cut(s) 67, 123, 132, 141, 204, 268, 275
CviQI GTAC 2 cut(s) 112, 126
DdeI CTNAG 3 cut(s) 63, 133, 278
DpnI GATC 2 cut(s) 101, 210
DpnII GATC 2 cut(s) 99, 208
Ecl136II GAGCTC 1 cut(s) 275
Eco105I TACGTA 1 cut(s) 111
Eco130I CCWWGG 1 cut(s) 115
Eco24I GRGCYC 1 cut(s) 277
Eco47I GGWCC 1 cut(s) 329
Eco53kI GAGCTC 1 cut(s) 275
EcoICRI GAGCTC 1 cut(s) 275
EcoO109I RGGNCCY 1 cut(s) 329
EcoRI GAATTC 1 cut(s) 161
EcoRII CCWGG 1 cut(s) 238
EcoT14I CCWWGG 1 cut(s) 115
EcoT38I GRGCYC 1 cut(s) 277
ErhI CCWWGG 1 cut(s) 115
FaiI YATR 1 cut(s) 174
Fnu4HI GCNGC 1 cut(s) 121
FokI GGATG 1 cut(s) 47
FriOI GRGCYC 1 cut(s) 277
Fsp4HI GCNGC 1 cut(s) 121
FspBI CTAG 3 cut(s) 68, 129, 188
GluI GCNGC 1 cut(s) 121
HapII CCGG 2 cut(s) 32, 337
HindIII AAGCTT 1 cut(s) 266
HinfI GANTC 1 cut(s) 247
HpaII CCGG 2 cut(s) 32, 337
HphI GGTGA 3 cut(s) 107, 326, 336
Hpy188I TCNGA 2 cut(s) 213, 228
Hpy188III TCNNGA 3 cut(s) 78, 188, 251
HpyAV CCTTC 2 cut(s) 146, 305
HpyCH4IV ACGT 1 cut(s) 110
HpyCH4V TGCA 1 cut(s) 145
HpyF10VI GCNNNNNNNGC 2 cut(s) 129, 138
HpyF3I CTNAG 3 cut(s) 63, 133, 278
HpySE526I ACGT 1 cut(s) 110
Kzo9I GATC 2 cut(s) 99, 208
LmnI GCTCC 1 cut(s) 272
LpnPI CCDG 9 cut(s) 45, 63, 72, 78, 155, 225, 252, 264, 312
Lsp1109I GCAGC 1 cut(s) 132
MaeI CTAG 3 cut(s) 68, 129, 188
MaeII ACGT 1 cut(s) 110
MalI GATC 2 cut(s) 101, 210
MboI GATC 2 cut(s) 99, 208
MflI RGATCY 1 cut(s) 99
MhlI GDGCHC 2 cut(s) 47, 277
MluCI AATT 4 cut(s) 10, 161, 254, 317
MnlI CCTC 2 cut(s) 189, 194
MseI TTAA 1 cut(s) 197
MspA1I CMGCKG 1 cut(s) 123
MspI CCGG 2 cut(s) 32, 337
MspR9I CCNGG 2 cut(s) 240, 337
MvaI CCWGG 1 cut(s) 240
MwoI GCNNNNNNNGC 2 cut(s) 129, 138
NciI CCSGG 1 cut(s) 337
NdeII GATC 2 cut(s) 99, 208
NlaIV GGNNCC 1 cut(s) 331
PfeI GAWTC 1 cut(s) 247
PkrI GCNGC 1 cut(s) 122
Ppu21I YACGTR 1 cut(s) 111
PpuMI RGGWCCY 1 cut(s) 329
Psp124BI GAGCTC 1 cut(s) 277
Psp5II RGGWCCY 1 cut(s) 329
Psp6I CCWGG 1 cut(s) 238
PspGI CCWGG 1 cut(s) 238
PspN4I GGNNCC 1 cut(s) 331
PspPI GGNCC 1 cut(s) 329
PspPPI RGGWCCY 1 cut(s) 329
PsuI RGATCY 1 cut(s) 99
PvuII CAGCTG 1 cut(s) 123
RsaI GTAC 2 cut(s) 113, 127
RsaNI GTAC 2 cut(s) 112, 126
SacI GAGCTC 1 cut(s) 277
SaqAI TTAA 1 cut(s) 197
SatI GCNGC 1 cut(s) 121
Sau3AI GATC 2 cut(s) 99, 208
Sau96I GGNCC 1 cut(s) 329
ScrFI CCNGG 2 cut(s) 240, 337
SduI GDGCHC 2 cut(s) 47, 277
SinI GGWCC 1 cut(s) 329
SnaBI TACGTA 1 cut(s) 111
Sse9I AATT 4 cut(s) 10, 161, 254, 317
SspMI CTAG 3 cut(s) 68, 129, 188
SstI GAGCTC 1 cut(s) 277
StyD4I CCNGG 2 cut(s) 238, 335
StyI CCWWGG 1 cut(s) 115
TaiI ACGT 1 cut(s) 113
TasI AATT 4 cut(s) 10, 161, 254, 317
TatI WGTACW 1 cut(s) 125
TfiI GAWTC 1 cut(s) 247
Tru1I TTAA 1 cut(s) 197
Tru9I TTAA 1 cut(s) 197
TseI GCWGC 1 cut(s) 120
TspDTI ATGAA 2 cut(s) 51, 174
VpaK11BI GGWCC 1 cut(s) 329
XapI RAATTY 3 cut(s) 10, 161, 254
XbaI TCTAGA 1 cut(s) 187
XspI CTAG 3 cut(s) 68, 129, 188
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.