MD04G1104100.v1.1

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr04
OG0017328
Physical Location & Seq
Reverse (-)
19127857 .. 19128821
965 bp
Loading structure...
UTR
Exon/CDS
Intron
MD04G1104100.v1.1.491

Sequence Viewer

Length: 945 bp
ATGGTCGAACGCTGCCTAATTGCAGCTTCTCTGGCAGCGTTGGTGTGCCATCCAAGCTCCACCATCAAGTGTTTCAAACCCAAGCCAAAACCGACATCCCCACAACAAGAACAACCAAAAGACAACCCTTCTCCTCCGCCGCAAGGGCCGAAACGACGTGACGTGATTGCAAAGGAAGTACCCTCAAAATCCAAGGTAAAACCATGCCCCAAGGGAGATTCTTCTTCGTCTCCTAGTCAGTCGAAAAGGAGCGCGAAGAAAACAGTCACGACACGGATTAATGCGGTCTCGGAGCTTGTCGTTGGAGATCCTTGGATCGGTGTTGTGGAGGATATTTTTCGGTGTAGTTGGGCGGGTGAAATAGACCTCAAAATTGAGAGGGTGTTGAGGGTGAATCATAGTTTGGATGTTCTTCATAGGTTTGAAGAGTATAGGAAAGTGGTGAAATCCAGGTATGAAAAGGGTCGGATTCTCAAGAGGATGGAGAGGTTGGTGGTTGATGGGAATGAGCTACTGCAATTCCATGGTGGCACCATTACATGTTCGCTAGGGATTAATGGATTCTCAAGTATCTGTCGTCGAAAATGCTGCCAAGTTTGCAGATTAATAGCCGGTGAGTTTTCCGGTGAAGGGTTGATGTCGTTGTTCGACAACAGTTGGAAGGCGCATGAGAAGATGAGCAAGGAATGTGCAGGGAAAGGAGTGTGTGCAAGTGCCAGGAAGGCTATTGTGGTGTGCAGAGTGATTGCAGGGCACGTTGCTCATTACTATGCACATGCACATGGAAGGTGGTCATTCATGGATAACGAAGAGGGCGAATTTGATTCTGTCGACTGGACTGCGGATCAATTTAGGGAATCGAAAGAACTCGTTGTTTTGAATTCGGAGGCAGTGCTTCCATGCTTTGTGATCATATATGATGTCAAAAATAAAAATCTTCCATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

315

Amino Acids

35.04

Weight (kDa)

9.13

Isoelectric Point (pI)

47.67

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0017328)

Species Orthologous Gene IDs
fragaria_vesca FvH4_6g15390
malus_domestica MD04G1104100.v1.1
prunus_persica Prupe.6G241000_v2.0.a1
rosa_chinensis RchiOBHm_Chr3g0468871
rosa_laevigata RLG00000024405
rosa_multiflora Rmu_sc0001639.1_g000016
rosa_roxburghii Rroxscaffold_2G00108650 Rroxscaffold_2G00108670 Rroxscaffold_6G00412500
rosa_samantha Rh3AG146900 Rh3BG169400 Rh3DG205800
rosa_wichuraiana Rw3G013810

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 530
AccI GTMKAC 1 cut(s) 831
AccII CGCG 1 cut(s) 254
AciI CCGC 5 cut(s) 137, 140, 284, 353, 842
AclWI GGATC 3 cut(s) 302, 323, 852
AcsI RAATTY 2 cut(s) 818, 880
AfaI GTAC 1 cut(s) 180
AfiI CCNNNNNNNGG 3 cut(s) 143, 317, 630
AflIII ACRYGT 1 cut(s) 539
AgsI TTSAA 3 cut(s) 76, 425, 880
AjiI CACGTC 2 cut(s) 158, 163
AjnI CCWGG 2 cut(s) 449, 716
AjuI GAANNNNNNNTTGG 2 cut(s) 386, 418
AluBI AGCT 4 cut(s) 26, 57, 295, 511
AluI AGCT 4 cut(s) 26, 57, 295, 511
Alw26I GTCTC 2 cut(s) 234, 292
AlwI GGATC 3 cut(s) 302, 323, 852
AoxI GGCC 1 cut(s) 146
ApeKI GCWGC 4 cut(s) 12, 23, 35, 588
ApoI RAATTY 2 cut(s) 818, 880
AseI ATTAAT 3 cut(s) 279, 555, 605
AspLEI GCGC 2 cut(s) 254, 667
AspS9I GGNCC 1 cut(s) 146
AsuHPI GGTGA 5 cut(s) 368, 403, 454, 626, 638
BaeGI GKGCMC 1 cut(s) 756
BanI GGYRCC 1 cut(s) 530
BbvI GCAGC 3 cut(s) 35, 47, 575
BccI CCATC 4 cut(s) 57, 71, 475, 494
BcgI CGANNNNNNTGC 4 cut(s) 570, 604, 821, 855
BciT130I CCWGG 2 cut(s) 451, 718
BclI TGATCA 1 cut(s) 909
BcoDI GTCTC 2 cut(s) 234, 292
BfaI CTAG 2 cut(s) 234, 548
BglI GCCNNNNNGGC 2 cut(s) 145, 722
BisI GCNGC 5 cut(s) 13, 24, 36, 140, 589
BlsI GCNGC 5 cut(s) 14, 25, 37, 141, 590
Bme1390I CCNGG 2 cut(s) 451, 718
BmgBI CACGTC 2 cut(s) 158, 163
BmgT120I GGNCC 1 cut(s) 146
BmiI GGNNCC 1 cut(s) 532
BmrFI CCNGG 2 cut(s) 451, 718
BpuEI CTTGAG 2 cut(s) 458, 550
BsaI GGTCTC 1 cut(s) 292
BsaJI CCNNGG 4 cut(s) 192, 210, 311, 523
BsaWI WCCGGW 1 cut(s) 623
Bsc4I CCNNNNNNNGG 3 cut(s) 143, 317, 630
Bse118I RCCGGY 1 cut(s) 611
Bse1I ACTGG 1 cut(s) 839
BseBI CCWGG 2 cut(s) 451, 718
BseDI CCNNGG 4 cut(s) 192, 210, 311, 523
BseGI GGATG 4 cut(s) 49, 95, 412, 486
BseLI CCNNNNNNNGG 3 cut(s) 143, 317, 630
BseNI ACTGG 1 cut(s) 839
BseRI GAGGAG 1 cut(s) 123
BseSI GKGCMC 1 cut(s) 756
BseXI GCAGC 3 cut(s) 35, 47, 575
BsgI GTGCAG 2 cut(s) 711, 757
Bsh1236I CGCG 1 cut(s) 254
BshFI GGCC 1 cut(s) 148
BshNI GGYRCC 1 cut(s) 530
BsiSI CCGG 2 cut(s) 612, 624
BslI CCNNNNNNNGG 3 cut(s) 143, 317, 630
BsmAI GTCTC 2 cut(s) 234, 292
BsmBI CGTCTC 1 cut(s) 234
BsnI GGCC 1 cut(s) 148
Bso31I GGTCTC 1 cut(s) 292
Bsp1286I GDGCHC 1 cut(s) 756
Bsp143I GATC 4 cut(s) 307, 315, 844, 909
Bsp19I CCATGG 1 cut(s) 523
BspACI CCGC 5 cut(s) 137, 140, 284, 353, 842
BspANI GGCC 1 cut(s) 148
BspFNI CGCG 1 cut(s) 254
BspLI GGNNCC 1 cut(s) 532
BspPI GGATC 3 cut(s) 302, 323, 852
BspT107I GGYRCC 1 cut(s) 530
BspTNI GGTCTC 1 cut(s) 292
BsrFI RCCGGY 1 cut(s) 611
BsrI ACTGG 1 cut(s) 839
BssAI RCCGGY 1 cut(s) 611
BssECI CCNNGG 4 cut(s) 192, 210, 311, 523
BssMI GATC 4 cut(s) 307, 315, 844, 909
BssT1I CCWWGG 4 cut(s) 192, 210, 311, 523
Bst2UI CCWGG 2 cut(s) 451, 718
Bst4CI ACNGT 2 cut(s) 265, 656
Bst6I CTCTTC 2 cut(s) 420, 804
BstDSI CCRYGG 1 cut(s) 523
BstF5I GGATG 4 cut(s) 49, 95, 412, 486
BstFNI CGCG 1 cut(s) 254
BstHHI GCGC 2 cut(s) 254, 667
BstKTI GATC 4 cut(s) 310, 318, 847, 912
BstMAI GTCTC 2 cut(s) 234, 292
BstMBI GATC 4 cut(s) 307, 315, 844, 909
BstMWI GCNNNNNNNGC 5 cut(s) 32, 54, 145, 597, 722
BstNI CCWGG 2 cut(s) 451, 718
BstNSI RCATGY 2 cut(s) 543, 779
BstSCI CCNGG 2 cut(s) 449, 716
BstSLI GKGCMC 1 cut(s) 756
BstUI CGCG 1 cut(s) 254
BstV1I GCAGC 3 cut(s) 35, 47, 575
BstX2I RGATCY 1 cut(s) 307
BstYI RGATCY 1 cut(s) 307
BsuRI GGCC 1 cut(s) 148
BtgI CCRYGG 1 cut(s) 523
BtrI CACGTC 2 cut(s) 158, 163
BtsCI GGATG 4 cut(s) 49, 95, 412, 486
BtsI GCAGTG 1 cut(s) 897
BtsIMutI CAGTG 1 cut(s) 897
CfoI GCGC 2 cut(s) 254, 667
Cfr10I RCCGGY 1 cut(s) 611
Cfr13I GGNCC 1 cut(s) 146
Csp6I GTAC 1 cut(s) 179
CviAII CATG 9 cut(s) 204, 524, 540, 668, 776, 782, 799, 900, 942
CviJI RGCY 8 cut(s) 26, 57, 85, 148, 295, 511, 611, 725
CviKI_1 RGCY 8 cut(s) 26, 57, 85, 148, 295, 511, 611, 725
CviQI GTAC 1 cut(s) 179
DpnI GATC 4 cut(s) 309, 317, 846, 911
DpnII GATC 4 cut(s) 307, 315, 844, 909
Eam1104I CTCTTC 2 cut(s) 420, 804
EarI CTCTTC 2 cut(s) 420, 804
EciI GGCGGA 1 cut(s) 126
Eco130I CCWWGG 4 cut(s) 192, 210, 311, 523
Eco31I GGTCTC 1 cut(s) 292
EcoRI GAATTC 1 cut(s) 880
EcoRII CCWGG 2 cut(s) 449, 716
EcoT14I CCWWGG 4 cut(s) 192, 210, 311, 523
ErhI CCWWGG 4 cut(s) 192, 210, 311, 523
Esp3I CGTCTC 1 cut(s) 234
FaeI CATG 9 cut(s) 207, 527, 543, 671, 779, 785, 802, 903, 945
FatI CATG 9 cut(s) 203, 523, 539, 667, 775, 781, 798, 899, 941
FauI CCCGC 1 cut(s) 346
FbaI TGATCA 1 cut(s) 909
FblI GTMKAC 1 cut(s) 831
Fnu4HI GCNGC 5 cut(s) 13, 24, 36, 140, 589
FokI GGATG 4 cut(s) 36, 82, 419, 493
Fsp4HI GCNGC 5 cut(s) 13, 24, 36, 140, 589
FspBI CTAG 2 cut(s) 234, 548
GlaI GCGC 2 cut(s) 253, 666
GluI GCNGC 5 cut(s) 13, 24, 36, 140, 589
HaeIII GGCC 1 cut(s) 148
HapII CCGG 2 cut(s) 612, 624
HhaI GCGC 2 cut(s) 254, 667
Hin1II CATG 9 cut(s) 207, 527, 543, 671, 779, 785, 802, 903, 945
Hin6I GCGC 2 cut(s) 252, 665
HinP1I GCGC 2 cut(s) 252, 665
HincII GTYRAC 1 cut(s) 832
HindII GTYRAC 1 cut(s) 832
HinfI GANTC 6 cut(s) 218, 394, 469, 561, 824, 857
HpaII CCGG 2 cut(s) 612, 624
HphI GGTGA 5 cut(s) 368, 403, 454, 626, 638
Hpy166II GTNNAC 1 cut(s) 832
Hpy188I TCNGA 3 cut(s) 292, 468, 886
Hpy188III TCNNGA 2 cut(s) 268, 475
Hpy8I GTNNAC 1 cut(s) 832
Hpy99I CGWCG 2 cut(s) 159, 582
HpyAV CCTTC 5 cut(s) 138, 623, 655, 715, 780
HpyCH4III ACNGT 2 cut(s) 265, 656
HpyCH4IV ACGT 3 cut(s) 157, 162, 756
HpyF10VI GCNNNNNNNGC 5 cut(s) 32, 54, 145, 597, 722
HpySE526I ACGT 3 cut(s) 157, 162, 756
Hsp92II CATG 9 cut(s) 207, 527, 543, 671, 779, 785, 802, 903, 945
HspAI GCGC 2 cut(s) 252, 665
Ksp22I TGATCA 1 cut(s) 909
Kzo9I GATC 4 cut(s) 307, 315, 844, 909
LmnI GCTCC 3 cut(s) 62, 249, 292
Lsp1109I GCAGC 3 cut(s) 35, 47, 575
MaeI CTAG 2 cut(s) 234, 548
MaeII ACGT 3 cut(s) 157, 162, 756
MaeIII GTNAC 2 cut(s) 158, 265
MalI GATC 4 cut(s) 309, 317, 846, 911
MboI GATC 4 cut(s) 307, 315, 844, 909
MboII GAAGA 8 cut(s) 213, 216, 268, 404, 437, 685, 821, 929
MflI RGATCY 1 cut(s) 307
MhlI GDGCHC 1 cut(s) 756
MluCI AATT 6 cut(s) 18, 372, 518, 818, 848, 880
MmeI TCCRAC 3 cut(s) 283, 446, 638
MseI TTAA 3 cut(s) 279, 555, 605
MslI CAYNNNNRTG 2 cut(s) 768, 780
MspI CCGG 2 cut(s) 612, 624
MspR9I CCNGG 2 cut(s) 451, 718
MvaI CCWGG 2 cut(s) 451, 718
MvnI CGCG 1 cut(s) 254
MwoI GCNNNNNNNGC 5 cut(s) 32, 54, 145, 597, 722
NcoI CCATGG 1 cut(s) 523
NdeII GATC 4 cut(s) 307, 315, 844, 909
NlaIII CATG 9 cut(s) 207, 527, 543, 671, 779, 785, 802, 903, 945
NlaIV GGNNCC 1 cut(s) 532
NmuCI GTSAC 2 cut(s) 158, 265
NspI RCATGY 2 cut(s) 543, 779
PciI ACATGT 1 cut(s) 539
PcsI WCGNNNNNNNCGW 1 cut(s) 813
PfeI GAWTC 6 cut(s) 218, 394, 469, 561, 824, 857
PkrI GCNGC 5 cut(s) 14, 25, 37, 141, 590
PscI ACATGT 1 cut(s) 539
PshBI ATTAAT 3 cut(s) 279, 555, 605
Psp6I CCWGG 2 cut(s) 449, 716
PspGI CCWGG 2 cut(s) 449, 716
PspN4I GGNNCC 1 cut(s) 532
PspPI GGNCC 1 cut(s) 146
PsuI RGATCY 1 cut(s) 307
RsaI GTAC 1 cut(s) 180
RsaNI GTAC 1 cut(s) 179
RseI CAYNNNNRTG 2 cut(s) 768, 780
SalI GTCGAC 1 cut(s) 830
SaqAI TTAA 3 cut(s) 279, 555, 605
SatI GCNGC 5 cut(s) 13, 24, 36, 140, 589
Sau3AI GATC 4 cut(s) 307, 315, 844, 909
Sau96I GGNCC 1 cut(s) 146
ScrFI CCNGG 2 cut(s) 451, 718
SduI GDGCHC 1 cut(s) 756
SmiMI CAYNNNNRTG 2 cut(s) 768, 780
SmlI CTYRAG 2 cut(s) 473, 565
SmoI CTYRAG 2 cut(s) 473, 565
Sse9I AATT 6 cut(s) 18, 372, 518, 818, 848, 880
SsiI CCGC 5 cut(s) 137, 140, 284, 353, 842
SspMI CTAG 2 cut(s) 234, 548
StyD4I CCNGG 2 cut(s) 449, 716
StyI CCWWGG 4 cut(s) 192, 210, 311, 523
TaaI ACNGT 2 cut(s) 265, 656
TaiI ACGT 3 cut(s) 160, 165, 759
TaqI TCGA 6 cut(s) 6, 242, 580, 648, 831, 860
TasI AATT 6 cut(s) 18, 372, 518, 818, 848, 880
TauI GCSGC 1 cut(s) 142
TfiI GAWTC 6 cut(s) 218, 394, 469, 561, 824, 857
Tru1I TTAA 3 cut(s) 279, 555, 605
Tru9I TTAA 3 cut(s) 279, 555, 605
TscAI CASTG 1 cut(s) 897
TseFI GTSAC 2 cut(s) 158, 265
TseI GCWGC 4 cut(s) 12, 23, 35, 588
Tsp45I GTSAC 2 cut(s) 158, 265
TspDTI ATGAA 3 cut(s) 404, 471, 787
TspGWI ACGGA 1 cut(s) 289
TspRI CASTG 1 cut(s) 897
VspI ATTAAT 3 cut(s) 279, 555, 605
XapI RAATTY 2 cut(s) 818, 880
XceI RCATGY 2 cut(s) 543, 779
XmiI GTMKAC 1 cut(s) 831
XspI CTAG 2 cut(s) 234, 548
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.