MD04G1116900.v1.1

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr04
OG0009822
Physical Location & Seq
Forward (+)
20218060 .. 20222754
4695 bp
Loading structure...
UTR
Exon/CDS
Intron
MD04G1116900.v1.1.491

Sequence Viewer

Length: 1140 bp
ATGTCGTGGATTAGAAATGCGGTGAACCTGGCAGTGGAAGCAGGGGCCGCCAACAGCCTCACCCGCACCGTACGCAACTACACCGACACTGTCGTCCTCCACGCGGGCAATGCCGTCGCCGGCGGAGCCAGACTCCTCCTCCCTGACCGCATTCGTCCAAGGAATTTACAGAGTTTTAAGCAGACTGTGAAAAGATTGGAAGAAGTTTCTGTTTCTTGTAGAGGTGTAGAAAGGGTTCAGCTGCTGAGGAGGTGGCTGGTTTCACTCAAAGAAATCGAGAGATTGTCCACCCTTTACAATGCGAGAAACGGAAAGGGTTCTGAAGACCACCTTACATCCGATGAGTCAGCAGACTCCCCGAGGAAACCTACCCTGGTTTATTACGTAGACTCCGATGCTGGAGATGAACCGAAAACTTTTCGTGATGTCTTTCTTCATAGCCAAGCTCTGGAGGGCATGACATTATCCATGATTCTTGAAGAACCAAATGAGGAAGAAGTTTCGCTACTTTTGGAGATATATGGGCTCTGTCTTACAGGAGGAAAGGAAGTACACAATGCAGTAATGAGCAGCGTACAGAATTTGTCAATTGCACTTTCATACTATCAAGATGAAGTACTGATAAAGCGAGAAGAATTGCTCCAATTTGCTCAAAGTGCAATTGCAGGGCTGAAAATAAATGCAGATCTTGCAAGAATAGATGCTGAAGCCTGCAGTTTAAGGGAAGAACTTGATAAATTGATGGCACACCAGAAGGCTCTTAGTGAAGGTTGTGAAAAATCAGCTGAGAAGACAACAGATGCGACAATAGAGGTAAGCTTGATGTTCTTATTTATGCAGATGCACGACATACGTATGCAAATCCACTCACATTTGCATGGAAATTTAAAAGACGCGCTTGTAGAAATTCAACTATGTTCCAGGTTGGAGGCTCTGTTACTAAAGAAGAAATCATTGAGCAATGGGGACTCACCCGAGATGCATTATGAAAAGGTTGAGAAATTGAAAATATTATCAGAATCTCTTGCTAACTCCACCTCACAAGCTGAAAAGCGCATTTCAGACCACAGGGATAGTGCACTTTGTGATCAATTCTTGTTTGGTGCATGTCAGGGCATGCATCTCCAATATGATATTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

380

Amino Acids

42.45

Weight (kDa)

5.62

Isoelectric Point (pI)

50.89

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 92
AccB7I CCANNNNNTGG 1 cut(s) 448
AccI GTMKAC 1 cut(s) 387
AccII CGCG 2 cut(s) 104, 896
AciI CCGC 6 cut(s) 20, 48, 64, 104, 123, 148
AcsI RAATTY 4 cut(s) 163, 580, 883, 906
AcuI CTGAAG 2 cut(s) 342, 726
AdeI CACNNNGTG 1 cut(s) 1085
AfaI GTAC 4 cut(s) 72, 552, 576, 618
AfiI CCNNNNNNNGG 3 cut(s) 34, 103, 448
AgsI TTSAA 3 cut(s) 479, 911, 1006
AjnI CCWGG 3 cut(s) 27, 372, 920
AluBI AGCT 5 cut(s) 241, 446, 785, 819, 1046
AluI AGCT 5 cut(s) 241, 446, 785, 819, 1046
Alw21I GWGCWC 1 cut(s) 1081
Alw44I GTGCAC 1 cut(s) 1077
AlwNI CAGNNNCTG 1 cut(s) 244
Ama87I CYCGRG 2 cut(s) 358, 974
AoxI GGCC 1 cut(s) 45
ApaLI GTGCAC 1 cut(s) 1077
ApeKI GCWGC 2 cut(s) 241, 570
ApoI RAATTY 4 cut(s) 163, 580, 883, 906
Asp700I GAANNNNTTC 2 cut(s) 234, 316
AspLEI GCGC 2 cut(s) 898, 1056
AspS9I GGNCC 1 cut(s) 45
AsuHPI GGTGA 3 cut(s) 34, 52, 963
AvaI CYCGRG 2 cut(s) 358, 974
BaeGI GKGCMC 1 cut(s) 1081
BanII GRGCYC 1 cut(s) 528
BarI GAAGNNNNNNTAC 2 cut(s) 489, 521
BbsI GAAGAC 2 cut(s) 330, 797
Bbv12I GWGCWC 1 cut(s) 1081
BbvCI CCTCAGC 1 cut(s) 245
BbvI GCAGC 2 cut(s) 228, 582
BccI CCATC 1 cut(s) 736
BceAI ACGGC 1 cut(s) 98
BcgI CGANNNNNNTGC 2 cut(s) 97, 131
BciT130I CCWGG 3 cut(s) 29, 374, 922
BclI TGATCA 1 cut(s) 1087
BfmI CTRYAG 1 cut(s) 712
BglII AGATCT 1 cut(s) 685
BisI GCNGC 3 cut(s) 48, 242, 571
BlsI GCNGC 3 cut(s) 49, 243, 572
BmcAI AGTACT 1 cut(s) 618
Bme1390I CCNGG 3 cut(s) 29, 374, 922
BmeT110I CYCGRG 2 cut(s) 358, 974
BmgT120I GGNCC 1 cut(s) 45
BmiI GGNNCC 2 cut(s) 46, 127
BmrFI CCNGG 3 cut(s) 29, 374, 922
BmsI GCATC 6 cut(s) 385, 691, 790, 831, 969, 1129
BpiI GAAGAC 2 cut(s) 330, 797
BplI GAGNNNNNCTC 2 cut(s) 117, 149
BpmI CTGGAG 2 cut(s) 420, 470
Bpu10I CCTNAGC 1 cut(s) 245
BsaAI YACGTR 2 cut(s) 385, 854
BsaJI CCNNGG 3 cut(s) 158, 359, 372
BsaXI ACNNNNNCTCC 6 cut(s) 123, 153, 374, 404, 920, 950
Bsc4I CCNNNNNNNGG 3 cut(s) 34, 103, 448
Bse118I RCCGGY 1 cut(s) 119
Bse3DI GCAATG 2 cut(s) 115, 967
BseBI CCWGG 3 cut(s) 29, 374, 922
BseDI CCNNGG 3 cut(s) 158, 359, 372
BseGI GGATG 1 cut(s) 335
BseLI CCNNNNNNNGG 3 cut(s) 34, 103, 448
BseMI GCAATG 2 cut(s) 115, 967
BseMII CTCAG 2 cut(s) 236, 777
BseRI GAGGAG 3 cut(s) 125, 128, 262
BseSI GKGCMC 1 cut(s) 1081
BseXI GCAGC 2 cut(s) 228, 582
Bsh1236I CGCG 2 cut(s) 104, 896
BshFI GGCC 1 cut(s) 47
BsiHKAI GWGCWC 1 cut(s) 1081
BsiHKCI CYCGRG 2 cut(s) 358, 974
BsiSI CCGG 1 cut(s) 120
BsiWI CGTACG 1 cut(s) 70
BslFI GGGAC 1 cut(s) 980
BslI CCNNNNNNNGG 3 cut(s) 34, 103, 448
BsmFI GGGAC 1 cut(s) 980
BsmI GAATGC 1 cut(s) 150
BsnI GGCC 1 cut(s) 47
BsoBI CYCGRG 2 cut(s) 358, 974
Bsp1286I GDGCHC 2 cut(s) 528, 1081
Bsp143I GATC 2 cut(s) 685, 1087
BspACI CCGC 6 cut(s) 20, 48, 64, 104, 123, 148
BspANI GGCC 1 cut(s) 47
BspCNI CTCAG 2 cut(s) 237, 778
BspFNI CGCG 2 cut(s) 104, 896
BspLI GGNNCC 2 cut(s) 46, 127
BspMAI CTGCAG 1 cut(s) 716
BsrDI GCAATG 2 cut(s) 115, 967
BsrFI RCCGGY 1 cut(s) 119
BssAI RCCGGY 1 cut(s) 119
BssECI CCNNGG 3 cut(s) 158, 359, 372
BssMI GATC 2 cut(s) 685, 1087
BssT1I CCWWGG 1 cut(s) 158
Bst2UI CCWGG 3 cut(s) 29, 374, 922
Bst4CI ACNGT 3 cut(s) 70, 91, 187
BstAPI GCANNNNNTGC 1 cut(s) 689
BstBAI YACGTR 2 cut(s) 385, 854
BstC8I GCNNGC 4 cut(s) 106, 121, 712, 1118
BstDEI CTNAG 3 cut(s) 245, 761, 786
BstF5I GGATG 1 cut(s) 335
BstFNI CGCG 2 cut(s) 104, 896
BstHHI GCGC 2 cut(s) 898, 1056
BstKTI GATC 2 cut(s) 688, 1090
BstMBI GATC 2 cut(s) 685, 1087
BstMWI GCNNNNNNNGC 8 cut(s) 38, 47, 63, 72, 110, 125, 656, 689
BstNI CCWGG 3 cut(s) 29, 374, 922
BstNSI RCATGY 2 cut(s) 1110, 1120
BstSCI CCNGG 3 cut(s) 27, 372, 920
BstSFI CTRYAG 1 cut(s) 712
BstSLI GKGCMC 1 cut(s) 1081
BstSNI TACGTA 2 cut(s) 385, 854
BstUI CGCG 2 cut(s) 104, 896
BstV1I GCAGC 2 cut(s) 228, 582
BstV2I GAAGAC 2 cut(s) 330, 797
BstX2I RGATCY 1 cut(s) 685
BstYI RGATCY 1 cut(s) 685
BsuRI GGCC 1 cut(s) 47
BtsCI GGATG 1 cut(s) 335
BtsI GCAGTG 1 cut(s) 39
BtsIMutI CAGTG 2 cut(s) 39, 87
Cac8I GCNNGC 4 cut(s) 106, 121, 712, 1118
CaiI CAGNNNCTG 1 cut(s) 244
CfoI GCGC 2 cut(s) 898, 1056
Cfr10I RCCGGY 1 cut(s) 119
Cfr13I GGNCC 1 cut(s) 45
CseI GACGC 1 cut(s) 902
Csp6I GTAC 4 cut(s) 71, 551, 575, 617
CviAII CATG 5 cut(s) 457, 469, 878, 1107, 1117
CviQI GTAC 4 cut(s) 71, 551, 575, 617
DdeI CTNAG 3 cut(s) 245, 761, 786
DpnI GATC 2 cut(s) 687, 1089
DpnII GATC 2 cut(s) 685, 1087
DraI TTTAAA 1 cut(s) 888
DraIII CACNNNGTG 1 cut(s) 1085
DrdI GACNNNNNNGTC 1 cut(s) 92
DseDI GACNNNNNNGTC 1 cut(s) 92
EciI GGCGGA 1 cut(s) 138
Eco105I TACGTA 2 cut(s) 385, 854
Eco130I CCWWGG 1 cut(s) 158
Eco24I GRGCYC 1 cut(s) 528
Eco57I CTGAAG 2 cut(s) 342, 726
Eco88I CYCGRG 2 cut(s) 358, 974
EcoRII CCWGG 3 cut(s) 27, 372, 920
EcoT14I CCWWGG 1 cut(s) 158
EcoT22I ATGCAT 2 cut(s) 984, 1122
EcoT38I GRGCYC 1 cut(s) 528
ErhI CCWWGG 1 cut(s) 158
FaeI CATG 5 cut(s) 460, 472, 881, 1110, 1120
FalI AAGNNNNNCTT 4 cut(s) 315, 347, 882, 914
FaqI GGGAC 1 cut(s) 980
FatI CATG 5 cut(s) 456, 468, 877, 1106, 1116
FauI CCCGC 2 cut(s) 71, 97
FbaI TGATCA 1 cut(s) 1087
FblI GTMKAC 1 cut(s) 387
Fnu4HI GCNGC 3 cut(s) 48, 242, 571
FokI GGATG 1 cut(s) 322
FriOI GRGCYC 1 cut(s) 528
Fsp4HI GCNGC 3 cut(s) 48, 242, 571
GlaI GCGC 2 cut(s) 897, 1055
GluI GCNGC 3 cut(s) 48, 242, 571
GsuI CTGGAG 2 cut(s) 420, 470
HaeIII GGCC 1 cut(s) 47
HapII CCGG 1 cut(s) 120
HgaI GACGC 1 cut(s) 902
HhaI GCGC 2 cut(s) 898, 1056
Hin1II CATG 5 cut(s) 460, 472, 881, 1110, 1120
Hin6I GCGC 2 cut(s) 896, 1054
HinP1I GCGC 2 cut(s) 896, 1054
HindIII AAGCTT 1 cut(s) 817
HinfI GANTC 7 cut(s) 132, 344, 353, 389, 472, 968, 1019
HpaII CCGG 1 cut(s) 120
HphI GGTGA 3 cut(s) 34, 52, 963
Hpy166II GTNNAC 5 cut(s) 25, 288, 388, 553, 1079
Hpy188I TCNGA 5 cut(s) 322, 340, 394, 1018, 1063
Hpy188III TCNNGA 5 cut(s) 277, 422, 449, 476, 608
Hpy8I GTNNAC 5 cut(s) 25, 288, 388, 553, 1079
Hpy99I CGWCG 1 cut(s) 119
HpyAV CCTTC 2 cut(s) 748, 761
HpyCH4III ACNGT 3 cut(s) 70, 91, 187
HpyCH4IV ACGT 2 cut(s) 384, 853
HpyF10VI GCNNNNNNNGC 8 cut(s) 38, 47, 63, 72, 110, 125, 656, 689
HpyF3I CTNAG 3 cut(s) 245, 761, 786
HpySE526I ACGT 2 cut(s) 384, 853
Hsp92II CATG 5 cut(s) 460, 472, 881, 1110, 1120
HspAI GCGC 2 cut(s) 896, 1054
KroI GCCGGC 1 cut(s) 119
KroNI GCCGGC 1 cut(s) 121
Ksp22I TGATCA 1 cut(s) 1087
Kzo9I GATC 2 cut(s) 685, 1087
LmnI GCTCC 2 cut(s) 125, 645
Lsp1109I GCAGC 2 cut(s) 228, 582
LweI GCATC 6 cut(s) 385, 691, 790, 831, 969, 1129
MaeII ACGT 2 cut(s) 384, 853
MaeIII GTNAC 1 cut(s) 936
MalI GATC 2 cut(s) 687, 1089
MboI GATC 2 cut(s) 685, 1087
MboII GAAGA 9 cut(s) 212, 335, 425, 491, 506, 644, 737, 802, 958
MfeI CAATTG 2 cut(s) 588, 660
MflI RGATCY 1 cut(s) 685
MhlI GDGCHC 2 cut(s) 528, 1081
MlyI GAGTC 5 cut(s) 126, 347, 353, 383, 962
MmeI TCCRAC 1 cut(s) 906
Mph1103I ATGCAT 2 cut(s) 984, 1122
MreI CGCCGGCG 1 cut(s) 119
MroNI GCCGGC 1 cut(s) 119
MroXI GAANNNNTTC 2 cut(s) 234, 316
MseI TTAA 3 cut(s) 177, 719, 887
MslI CAYNNNNRTG 2 cut(s) 854, 876
MspA1I CMGCKG 2 cut(s) 241, 785
MspI CCGG 1 cut(s) 120
MspR9I CCNGG 3 cut(s) 29, 374, 922
MunI CAATTG 2 cut(s) 588, 660
Mva1269I GAATGC 1 cut(s) 150
MvaI CCWGG 3 cut(s) 29, 374, 922
MvnI CGCG 2 cut(s) 104, 896
MwoI GCNNNNNNNGC 8 cut(s) 38, 47, 63, 72, 110, 125, 656, 689
NaeI GCCGGC 1 cut(s) 121
NdeII GATC 2 cut(s) 685, 1087
NgoMIV GCCGGC 1 cut(s) 119
NlaIII CATG 5 cut(s) 460, 472, 881, 1110, 1120
NlaIV GGNNCC 2 cut(s) 46, 127
NsiI ATGCAT 2 cut(s) 984, 1122
NspI RCATGY 2 cut(s) 1110, 1120
PaeI GCATGC 1 cut(s) 1120
PcsI WCGNNNNNNNCGW 1 cut(s) 390
PctI GAATGC 1 cut(s) 150
PdiI GCCGGC 1 cut(s) 121
PdmI GAANNNNTTC 2 cut(s) 234, 316
PfeI GAWTC 2 cut(s) 472, 1019
Pfl23II CGTACG 1 cut(s) 70
PflFI GACNNNGTC 1 cut(s) 89
PflMI CCANNNNNTGG 1 cut(s) 448
PkrI GCNGC 3 cut(s) 49, 243, 572
PleI GAGTC 5 cut(s) 126, 347, 352, 383, 962
PpsI GAGTC 5 cut(s) 126, 347, 352, 383, 962
Ppu21I YACGTR 2 cut(s) 385, 854
Psp6I CCWGG 3 cut(s) 27, 372, 920
PspGI CCWGG 3 cut(s) 27, 372, 920
PspLI CGTACG 1 cut(s) 70
PspN4I GGNNCC 2 cut(s) 46, 127
PspPI GGNCC 1 cut(s) 45
PsrI GAACNNNNNNTAC 2 cut(s) 219, 251
PstI CTGCAG 1 cut(s) 716
PstNI CAGNNNCTG 1 cut(s) 244
PsuI RGATCY 1 cut(s) 685
PsyI GACNNNGTC 1 cut(s) 89
PvuII CAGCTG 2 cut(s) 241, 785
RsaI GTAC 4 cut(s) 72, 552, 576, 618
RsaNI GTAC 4 cut(s) 71, 551, 575, 617
RseI CAYNNNNRTG 2 cut(s) 854, 876
SaqAI TTAA 3 cut(s) 177, 719, 887
SatI GCNGC 3 cut(s) 48, 242, 571
Sau3AI GATC 2 cut(s) 685, 1087
Sau96I GGNCC 1 cut(s) 45
ScaI AGTACT 1 cut(s) 618
SchI GAGTC 5 cut(s) 126, 347, 353, 383, 962
ScrFI CCNGG 3 cut(s) 29, 374, 922
SduI GDGCHC 2 cut(s) 528, 1081
SfaNI GCATC 6 cut(s) 385, 691, 790, 831, 969, 1129
SfcI CTRYAG 1 cut(s) 712
SgrAI CRCCGGYG 1 cut(s) 119
SmiMI CAYNNNNRTG 2 cut(s) 854, 876
SnaBI TACGTA 2 cut(s) 385, 854
SphI GCATGC 1 cut(s) 1120
SsiI CCGC 6 cut(s) 20, 48, 64, 104, 123, 148
SspI AATATT 1 cut(s) 1011
StyD4I CCNGG 3 cut(s) 27, 372, 920
StyI CCWWGG 1 cut(s) 158
TaaI ACNGT 3 cut(s) 70, 91, 187
TaiI ACGT 2 cut(s) 387, 856
TaqI TCGA 1 cut(s) 276
TatI WGTACW 2 cut(s) 550, 616
TauI GCSGC 1 cut(s) 50
TfiI GAWTC 2 cut(s) 472, 1019
Tru1I TTAA 3 cut(s) 177, 719, 887
Tru9I TTAA 3 cut(s) 177, 719, 887
TscAI CASTG 2 cut(s) 39, 94
TseI GCWGC 2 cut(s) 241, 570
TspDTI ATGAA 5 cut(s) 420, 425, 588, 627, 1002
TspGWI ACGGA 1 cut(s) 324
TspRI CASTG 2 cut(s) 39, 94
Tth111I GACNNNGTC 1 cut(s) 89
Van91I CCANNNNNTGG 1 cut(s) 448
VneI GTGCAC 1 cut(s) 1077
XapI RAATTY 4 cut(s) 163, 580, 883, 906
XceI RCATGY 2 cut(s) 1110, 1120
XmiI GTMKAC 1 cut(s) 387
XmnI GAANNNNTTC 2 cut(s) 234, 316
ZrmI AGTACT 1 cut(s) 618
Zsp2I ATGCAT 2 cut(s) 984, 1122
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.