Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr04

Orthogroup ID

OG0006552

Physical Location & Seq

Genomic Coordinates Forward (+)

20368948 .. 20370906

Gene Structure 1959 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

MD04G1118300.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 903 bp

ATGAAGATGAAGGGCATAGACATCTTCTGTGCATCCCAAGCTTCGACAGCCATATGCATGAGCATGGATAATCAAGCATCCTCCTCTTCCTCCTCCGTCATTCAACTTGGAGGCCGAGCCCTCGACCGCCACAACCCCATCATCCAAGATGCAAGAAGAAGCACAAGCTCCAGAACCCTTCCCATTGCTCCATGCTCTTCCCAATCTCCCATCAATCCCAAAGCCTACCACCAAATCCCAAAGAGCAAGAAAAAAACCTCCTCATCACCAAGACCAAGCACTGATCATCAAAACAAGAAAAAGAGCTCCACAAAAGAAAACGACCAGAAGAGGAAGAGTAGTAATCTTTTACAAGGAAAGTTGGATGAAAATGTGAAGAAGTTTTCTTCTGTTCCAAGTGAGAGTGTTGTTGTGAGGAAGAGCTCTGCAAACCCTACTGATCTCATCACTCCTCCTGGCTCCACCAGATACCTTTTGAGTGATACTGTGATCTTTGATGGGTTATCCGATTACGATCCGGTTTCGGCATTGGTTCCAGTTGGGCAGAAAAAGAGCCAAAATGCAGTAAAAATACTCAAAGAAAATGAATCTACTACTTCTCCAATTAGCTCTTCCTCATCTCTGACAAAAGACTCACAACCACCGCCTCTGCCGCCCCCCCCTTCCAACCAGGTTGTTGTATTGATGGTGTCTCTCCACTGCAAAGGGTGTGTAGGAAAACTGAGAAAACATCTATCCAGAATGGAAGGGGTGACATCATTCAACATAGACTTTGCAGCAAAGAAGGTGACAGTCACTGGAGATGTAACCCCATTGAGTGTCCTTGCCAGTGTCTCAAAGGTGAAGAACGCTCAGTTTTGGCCTACTGTGTCATCAGCATCACGTGCCTCTCCACTAATTTAA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000003 GO:0003006 GO:0003674 GO:0005488 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005737 GO:0006810 GO:0006811 GO:0006812 GO:0006873 GO:0006875 GO:0007275 GO:0008150 GO:0009653 GO:0009791 GO:0009908 GO:0009987 GO:0010015 GO:0010232 GO:0010233 GO:0019725 GO:0022414 GO:0022622 GO:0030001 GO:0030003 GO:0032501 GO:0032502 GO:0042592 GO:0043167 GO:0043169 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044424 GO:0044464 GO:0046872 GO:0046914 GO:0046916 GO:0048364 GO:0048367 GO:0048608 GO:0048731 GO:0048856 GO:0048878 GO:0050801 GO:0051179 GO:0051234 GO:0055065 GO:0055067 GO:0055076 GO:0055078 GO:0055080 GO:0055082 GO:0061458 GO:0065007 GO:0065008 GO:0090567 GO:0098771 GO:0099402 GO:1905392

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

HMA

Protein Analysis

301

Amino Acids

32.17

Weight (kDa)

9.92

Isoelectric Point (pI)

55.5

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
HMA	PF00403	228 - 274	5e-13	Heavy-metal-associated domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

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Publication-ready

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Orthologous Genes (Group: OG0006552)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT2G37390 AT2G37390 AT3G53530 AT3G53530
fragaria_vesca	FvH4_6g14630 FvH4_6g14630 FvH4_6g14630 FvH4_6g14630 FvH4_6g14630 FvH4_6g14630
malus_domestica	MD04G1118300.v1.1 MD12G1133800.v1.1
prunus_persica	Prupe.6G247400_v2.0.a1 Prupe.6G247400_v2.0.a1
pyrus_communis	pycom04g10790 pycom12g12910
rosa_chinensis	RchiOBHm_Chr3g0467751
rosa_laevigata	RLG00000024476
rosa_multiflora	Rmu_sc0001793.1_g000025
rosa_roxburghii	Rroxscaffold_6G00413450
rosa_rugosa	Rorug03G0089600
rosa_samantha	Rh3AG138000 Rh3BG159300 Rh3CG159200 Rh3DG159700
rosa_wichuraiana	Rw3G013070

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AciI	CCGC	3 cut(s)	127, 644, 653
AclWI	GGATC	1 cut(s)	509
AcvI	CACGTG	1 cut(s)	884
AgsI	TTSAA	2 cut(s)	104, 763
AjnI	CCWGG	2 cut(s)	454, 669
AjuI	GAANNNNNNNTTGG	2 cut(s)	595, 627
AluBI	AGCT	5 cut(s)	41, 168, 306, 423, 609
AluI	AGCT	5 cut(s)	41, 168, 306, 423, 609
Alw21I	GWGCWC	2 cut(s)	308, 425
Alw26I	GTCTC	2 cut(s)	696, 838
AlwI	GGATC	1 cut(s)	509
AlwNI	CAGNNNCTG	1 cut(s)	797
AoxI	GGCC	2 cut(s)	112, 860
ApeKI	GCWGC	1 cut(s)	776
AsuHPI	GGTGA	4 cut(s)	258, 763, 799, 853
BanII	GRGCYC	3 cut(s)	121, 308, 425
BbrPI	CACGTG	1 cut(s)	884
Bbv12I	GWGCWC	2 cut(s)	308, 425
BbvI	GCAGC	1 cut(s)	788
BccI	CCATC	4 cut(s)	146, 218, 491, 679
BciT130I	CCWGG	2 cut(s)	456, 671
BclI	TGATCA	1 cut(s)	283
BcoDI	GTCTC	2 cut(s)	696, 838
BisI	GCNGC	2 cut(s)	653, 777
BlsI	GCNGC	2 cut(s)	654, 778
Bme1390I	CCNGG	2 cut(s)	456, 671
BmiI	GGNNCC	2 cut(s)	460, 534
BmrFI	CCNGG	2 cut(s)	456, 671
BmsI	GCATC	4 cut(s)	41, 86, 139, 887
BpmI	CTGGAG	2 cut(s)	154, 819
BsaAI	YACGTR	1 cut(s)	884
BsaBI	GATNNNNATC	1 cut(s)	513
BsaWI	WCCGGW	1 cut(s)	517
BsaXI	ACNNNNNCTCC	2 cut(s)	583, 613
Bse1I	ACTGG	3 cut(s)	536, 802, 828
Bse3DI	GCAATG	1 cut(s)	183
Bse8I	GATNNNNATC	1 cut(s)	513
BseBI	CCWGG	2 cut(s)	456, 671
BseGI	GGATG	4 cut(s)	32, 77, 141, 370
BseJI	GATNNNNATC	1 cut(s)	513
BseMI	GCAATG	1 cut(s)	183
BseMII	CTCAG	2 cut(s)	713, 866
BseNI	ACTGG	3 cut(s)	536, 802, 828
BseRI	GAGGAG	4 cut(s)	73, 82, 250, 441
BseXI	GCAGC	1 cut(s)	788
Bsh1285I	CGRYCG	1 cut(s)	127
BshFI	GGCC	2 cut(s)	114, 862
BsiEI	CGRYCG	1 cut(s)	127
BsiHKAI	GWGCWC	2 cut(s)	308, 425
BsiSI	CCGG	1 cut(s)	518
BsmAI	GTCTC	2 cut(s)	696, 838
BsnI	GGCC	2 cut(s)	114, 862
Bsp1286I	GDGCHC	3 cut(s)	121, 308, 425
Bsp143I	GATC	4 cut(s)	283, 439, 489, 514
BspACI	CCGC	3 cut(s)	127, 644, 653
BspANI	GGCC	2 cut(s)	114, 862
BspCNI	CTCAG	2 cut(s)	714, 865
BspLI	GGNNCC	2 cut(s)	460, 534
BspPI	GGATC	1 cut(s)	509
BspQI	GCTCTTC	3 cut(s)	202, 413, 616
BsrDI	GCAATG	1 cut(s)	183
BsrI	ACTGG	3 cut(s)	536, 802, 828
BssMI	GATC	4 cut(s)	283, 439, 489, 514
Bst2UI	CCWGG	2 cut(s)	456, 671
Bst4CI	ACNGT	3 cut(s)	487, 793, 868
Bst6I	CTCTTC	6 cut(s)	91, 202, 323, 329, 413, 616
BstAPI	GCANNNNNTGC	1 cut(s)	884
BstBAI	YACGTR	1 cut(s)	884
BstDEI	CTNAG	2 cut(s)	722, 852
BstF5I	GGATG	4 cut(s)	32, 77, 141, 370
BstKTI	GATC	4 cut(s)	286, 442, 492, 517
BstMAI	GTCTC	2 cut(s)	696, 838
BstMBI	GATC	4 cut(s)	283, 439, 489, 514
BstMCI	CGRYCG	1 cut(s)	127
BstMWI	GCNNNNNNNGC	4 cut(s)	38, 47, 652, 884
BstNI	CCWGG	2 cut(s)	456, 671
BstSCI	CCNGG	2 cut(s)	454, 669
BstV1I	GCAGC	1 cut(s)	788
BsuRI	GGCC	2 cut(s)	114, 862
BtsCI	GGATG	4 cut(s)	32, 77, 141, 370
BtsI	GCAGTG	1 cut(s)	697
BtsIMutI	CAGTG	4 cut(s)	279, 697, 795, 835
CaiI	CAGNNNCTG	1 cut(s)	797
CsiI	ACCWGGT	1 cut(s)	669
CviAII	CATG	3 cut(s)	58, 64, 192
DdeI	CTNAG	2 cut(s)	722, 852
DpnI	GATC	4 cut(s)	285, 441, 491, 516
DpnII	GATC	4 cut(s)	283, 439, 489, 514
Eam1104I	CTCTTC	6 cut(s)	91, 202, 323, 329, 413, 616
EarI	CTCTTC	6 cut(s)	91, 202, 323, 329, 413, 616
Ecl136II	GAGCTC	2 cut(s)	306, 423
Eco24I	GRGCYC	3 cut(s)	121, 308, 425
Eco53kI	GAGCTC	2 cut(s)	306, 423
Eco72I	CACGTG	1 cut(s)	884
EcoICRI	GAGCTC	2 cut(s)	306, 423
EcoRII	CCWGG	2 cut(s)	454, 669
EcoT22I	ATGCAT	1 cut(s)	59
EcoT38I	GRGCYC	3 cut(s)	121, 308, 425
FaeI	CATG	3 cut(s)	61, 67, 195
FaiI	YATR	7 cut(s)	17, 53, 55, 59, 65, 193, 767
FatI	CATG	3 cut(s)	57, 63, 191
FauNDI	CATATG	1 cut(s)	53
FbaI	TGATCA	1 cut(s)	283
Fnu4HI	GCNGC	2 cut(s)	653, 777
FokI	GGATG	4 cut(s)	19, 64, 128, 377
FriOI	GRGCYC	3 cut(s)	121, 308, 425
Fsp4HI	GCNGC	2 cut(s)	653, 777
GluI	GCNGC	2 cut(s)	653, 777
GsuI	CTGGAG	2 cut(s)	154, 819
HaeIII	GGCC	2 cut(s)	114, 862
HapII	CCGG	1 cut(s)	518
Hin1II	CATG	3 cut(s)	61, 67, 195
HindIII	AAGCTT	1 cut(s)	39
HinfI	GANTC	2 cut(s)	587, 632
HpaII	CCGG	1 cut(s)	518
HphI	GGTGA	4 cut(s)	258, 763, 799, 853
Hpy188I	TCNGA	2 cut(s)	508, 624
Hpy188III	TCNNGA	2 cut(s)	171, 738
HpyAV	CCTTC	5 cut(s)	4, 188, 672, 740, 778
HpyCH4III	ACNGT	3 cut(s)	487, 793, 868
HpyCH4IV	ACGT	1 cut(s)	883
HpyCH4V	TGCA	7 cut(s)	32, 57, 152, 428, 563, 702, 776
HpyF10VI	GCNNNNNNNGC	4 cut(s)	38, 47, 652, 884
HpyF3I	CTNAG	2 cut(s)	722, 852
HpySE526I	ACGT	1 cut(s)	883
Hsp92II	CATG	3 cut(s)	61, 67, 195
Ksp22I	TGATCA	1 cut(s)	283
Kzo9I	GATC	4 cut(s)	283, 439, 489, 514
LguI	GCTCTTC	3 cut(s)	202, 413, 616
LmnI	GCTCC	4 cut(s)	173, 193, 311, 464
Lsp1109I	GCAGC	1 cut(s)	788
LweI	GCATC	4 cut(s)	41, 86, 139, 887
MabI	ACCWGGT	1 cut(s)	669
MaeII	ACGT	1 cut(s)	883
MaeIII	GTNAC	4 cut(s)	751, 787, 793, 805
MalI	GATC	4 cut(s)	285, 441, 491, 516
MboI	GATC	4 cut(s)	283, 439, 489, 514
MhlI	GDGCHC	3 cut(s)	121, 308, 425
MluCI	AATT	2 cut(s)	603, 897
MlyI	GAGTC	1 cut(s)	626
MmeI	TCCRAC	2 cut(s)	342, 690
Mph1103I	ATGCAT	1 cut(s)	59
MseI	TTAA	1 cut(s)	901
MslI	CAYNNNNRTG	2 cut(s)	56, 62
MspI	CCGG	1 cut(s)	518
MspR9I	CCNGG	2 cut(s)	456, 671
MvaI	CCWGG	2 cut(s)	456, 671
MwoI	GCNNNNNNNGC	4 cut(s)	38, 47, 652, 884
NdeI	CATATG	1 cut(s)	53
NdeII	GATC	4 cut(s)	283, 439, 489, 514
NlaIII	CATG	3 cut(s)	61, 67, 195
NlaIV	GGNNCC	2 cut(s)	460, 534
NmeAIII	GCCGAG	1 cut(s)	140
NmuCI	GTSAC	3 cut(s)	751, 787, 793
NsiI	ATGCAT	1 cut(s)	59
PciSI	GCTCTTC	3 cut(s)	202, 413, 616
PfeI	GAWTC	1 cut(s)	587
PkrI	GCNGC	2 cut(s)	654, 778
PleI	GAGTC	1 cut(s)	626
PmaCI	CACGTG	1 cut(s)	884
PmlI	CACGTG	1 cut(s)	884
PpsI	GAGTC	1 cut(s)	626
Ppu21I	YACGTR	1 cut(s)	884
Psp124BI	GAGCTC	2 cut(s)	308, 425
Psp6I	CCWGG	2 cut(s)	454, 669
PspCI	CACGTG	1 cut(s)	884
PspGI	CCWGG	2 cut(s)	454, 669
PspN4I	GGNNCC	2 cut(s)	460, 534
PstNI	CAGNNNCTG	1 cut(s)	797
RseI	CAYNNNNRTG	2 cut(s)	56, 62
SacI	GAGCTC	2 cut(s)	308, 425
SapI	GCTCTTC	3 cut(s)	202, 413, 616
SaqAI	TTAA	1 cut(s)	901
SatI	GCNGC	2 cut(s)	653, 777
Sau3AI	GATC	4 cut(s)	283, 439, 489, 514
SchI	GAGTC	1 cut(s)	626
ScrFI	CCNGG	2 cut(s)	456, 671
SduI	GDGCHC	3 cut(s)	121, 308, 425
SexAI	ACCWGGT	1 cut(s)	669
SfaNI	GCATC	4 cut(s)	41, 86, 139, 887
SmiMI	CAYNNNNRTG	2 cut(s)	56, 62
Sse9I	AATT	2 cut(s)	603, 897
SsiI	CCGC	3 cut(s)	127, 644, 653
SstI	GAGCTC	2 cut(s)	308, 425
StyD4I	CCNGG	2 cut(s)	454, 669
TaaI	ACNGT	3 cut(s)	487, 793, 868
TaiI	ACGT	1 cut(s)	886
TaqI	TCGA	2 cut(s)	44, 123
TasI	AATT	2 cut(s)	603, 897
TauI	GCSGC	1 cut(s)	655
TfiI	GAWTC	1 cut(s)	587
Tru1I	TTAA	1 cut(s)	901
Tru9I	TTAA	1 cut(s)	901
TscAI	CASTG	4 cut(s)	286, 704, 802, 835
TseFI	GTSAC	3 cut(s)	751, 787, 793
TseI	GCWGC	1 cut(s)	776
Tsp45I	GTSAC	3 cut(s)	751, 787, 793
TspDTI	ATGAA	4 cut(s)	17, 23, 381, 600
TspGWI	ACGGA	1 cut(s)	85
TspRI	CASTG	4 cut(s)	286, 704, 802, 835
Zsp2I	ATGCAT	1 cut(s)	59

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.