Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr12

Orthogroup ID

OG0006552

Physical Location & Seq

Genomic Coordinates Forward (+)

20921112 .. 20923109

Gene Structure 1998 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

MD12G1133800.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 906 bp

ATGAAGATGAAAGGCATAGACATATTCTGTGCATCCCAAGCTTCAACAGCCATATGCATGAACATGGATCATCAAGCATCCTCCTCCATCGTACAACTCGGCGGCCGAGCCCTCGACCGCCACAACCCCATCATCCAAGATGCAAGAAGAAGCACAAGCTCCAGAACCCTTCCCATTGCTCCATGCTCTTCCCAATCCCCCATCAACCCCAAGGCCTACCACCAACTCCCAAAGAACAAGAAAAAAGCAACCTCCTCGTCACTTAGACCAAGTACCAAGAGCTCCTCAAAAGAAAATGATCAGAAGAGGAAGAGTACTAGTAATATTTTTCATGGAAAGTTGGATGAAAATGTTAAGAAGTTTTCTTCTGTTCTAAGTGAGAGTGTTGTTAGGAAGAGCTCTGCCAACCCTATCGATCTTATTACTCCTCCTGGCTCCTCGAGATCCCTTTTGAGTGATACTGTGATCTTTGATGGGTTATCGGATTACGATCCGGTTTTGGCATTGGTTCCGGTTGGGCAGAAAAAGAATGCAGTGAAAATAATCACAGAAAATAAATCTACTACCTCTTCAATTGGCTCTTCTTCCTCATCTCTGACAAAAGACAAAAAACCACCAACGCCACCGCCACTGCCACCGCCTTCCAGCCAGGTTGTTGTATTGATGGTGTCCCTTCACTGCAAAGGGTGTGTAGGAAAACTGAGAAAACATCTATCCAGAATGGAAGGGGTGACATCATTCAACATAGACTTCGCAGCAAAGAAGGTGACAGTCACTGGAGATGTAACCCCATTGAGTGTCCTTGCCAGTGTCTCAAAGGTGAAGAACGCTCAGTTTTGGCCTACTGTGTCATCAGCATCACCTGCCTCTCCACCTTGTCCTACCAAACTAGAGGCCAAGAAATAA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000003 GO:0003006 GO:0003674 GO:0005488 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005737 GO:0006810 GO:0006811 GO:0006812 GO:0006873 GO:0006875 GO:0007275 GO:0008150 GO:0009653 GO:0009791 GO:0009908 GO:0009987 GO:0010015 GO:0010232 GO:0010233 GO:0019725 GO:0022414 GO:0022622 GO:0030001 GO:0030003 GO:0032501 GO:0032502 GO:0042592 GO:0043167 GO:0043169 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044424 GO:0044464 GO:0046872 GO:0046914 GO:0046916 GO:0048364 GO:0048367 GO:0048608 GO:0048731 GO:0048856 GO:0048878 GO:0050801 GO:0051179 GO:0051234 GO:0055065 GO:0055067 GO:0055076 GO:0055078 GO:0055080 GO:0055082 GO:0061458 GO:0065007 GO:0065008 GO:0090567 GO:0098771 GO:0099402 GO:1905392

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

HMA

Protein Analysis

302

Amino Acids

32.15

Weight (kDa)

9.97

Isoelectric Point (pI)

54.1

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
HMA	PF00403	221 - 267	5.1e-13	Heavy-metal-associated domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

Style Settings

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0006552)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT2G37390 AT2G37390 AT3G53530 AT3G53530
fragaria_vesca	FvH4_6g14630 FvH4_6g14630 FvH4_6g14630 FvH4_6g14630 FvH4_6g14630 FvH4_6g14630
malus_domestica	MD04G1118300.v1.1 MD12G1133800.v1.1
prunus_persica	Prupe.6G247400_v2.0.a1 Prupe.6G247400_v2.0.a1
pyrus_communis	pycom04g10790 pycom12g12910
rosa_chinensis	RchiOBHm_Chr3g0467751
rosa_laevigata	RLG00000024476
rosa_multiflora	Rmu_sc0001793.1_g000025
rosa_roxburghii	Rroxscaffold_6G00413450
rosa_rugosa	Rorug03G0089600
rosa_samantha	Rh3AG138000 Rh3BG159300 Rh3CG159200 Rh3DG159700
rosa_wichuraiana	Rw3G013070

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AarI	CACCTGC	1 cut(s)	871
Acc36I	ACCTGC	1 cut(s)	871
AciI	CCGC	4 cut(s)	102, 118, 626, 638
AclWI	GGATC	3 cut(s)	75, 438, 485
AcoI	YGGCCR	1 cut(s)	103
AfaI	GTAC	3 cut(s)	93, 274, 316
AgsI	TTSAA	3 cut(s)	45, 573, 742
AhlI	ACTAGT	1 cut(s)	317
AjnI	CCWGG	2 cut(s)	430, 648
AluBI	AGCT	4 cut(s)	41, 159, 282, 399
AluI	AGCT	4 cut(s)	41, 159, 282, 399
Alw21I	GWGCWC	2 cut(s)	284, 401
Alw26I	GTCTC	1 cut(s)	817
AlwI	GGATC	3 cut(s)	75, 438, 485
AlwNI	CAGNNNCTG	1 cut(s)	776
Ama87I	CYCGRG	1 cut(s)	439
AoxI	GGCC	4 cut(s)	103, 213, 839, 894
ApeKI	GCWGC	1 cut(s)	755
AsuHPI	GGTGA	4 cut(s)	742, 778, 832, 852
AvaI	CYCGRG	1 cut(s)	439
BanII	GRGCYC	3 cut(s)	112, 284, 401
Bbv12I	GWGCWC	2 cut(s)	284, 401
BbvI	GCAGC	1 cut(s)	767
BccI	CCATC	5 cut(s)	95, 137, 209, 467, 658
BcgI	CGANNNNNNTGC	2 cut(s)	237, 271
BciT130I	CCWGG	2 cut(s)	432, 650
BclI	TGATCA	1 cut(s)	298
BcoDI	GTCTC	1 cut(s)	817
BcuI	ACTAGT	1 cut(s)	317
BfaI	CTAG	2 cut(s)	318, 890
BfuAI	ACCTGC	1 cut(s)	871
BisI	GCNGC	2 cut(s)	103, 756
BlsI	GCNGC	2 cut(s)	104, 757
BmcAI	AGTACT	1 cut(s)	316
Bme1390I	CCNGG	2 cut(s)	432, 650
BmeT110I	CYCGRG	1 cut(s)	439
BmiI	GGNNCC	2 cut(s)	436, 510
BmrFI	CCNGG	2 cut(s)	432, 650
BmsI	GCATC	4 cut(s)	41, 86, 130, 866
BpmI	CTGGAG	2 cut(s)	145, 798
Bsa29I	ATCGAT	1 cut(s)	414
BsaBI	GATNNNNATC	1 cut(s)	489
BsaJI	CCNNGG	1 cut(s)	210
BsaWI	WCCGGW	2 cut(s)	493, 511
Bse1I	ACTGG	2 cut(s)	781, 807
Bse3DI	GCAATG	1 cut(s)	174
Bse8I	GATNNNNATC	1 cut(s)	489
BseBI	CCWGG	2 cut(s)	432, 650
BseCI	ATCGAT	1 cut(s)	414
BseDI	CCNNGG	1 cut(s)	210
BseGI	GGATG	4 cut(s)	32, 77, 132, 349
BseJI	GATNNNNATC	1 cut(s)	489
BseMI	GCAATG	1 cut(s)	174
BseMII	CTCAG	2 cut(s)	692, 845
BseNI	ACTGG	2 cut(s)	781, 807
BseRI	GAGGAG	5 cut(s)	73, 244, 274, 417, 427
BseX3I	CGGCCG	1 cut(s)	103
BseXI	GCAGC	1 cut(s)	767
Bsh1285I	CGRYCG	2 cut(s)	106, 118
BshFI	GGCC	4 cut(s)	105, 215, 841, 896
BshVI	ATCGAT	1 cut(s)	414
BsiEI	CGRYCG	2 cut(s)	106, 118
BsiHKAI	GWGCWC	2 cut(s)	284, 401
BsiHKCI	CYCGRG	1 cut(s)	439
BsiSI	CCGG	2 cut(s)	494, 512
BslFI	GGGAC	1 cut(s)	655
BsmAI	GTCTC	1 cut(s)	817
BsmFI	GGGAC	1 cut(s)	655
BsmI	GAATGC	1 cut(s)	535
BsnI	GGCC	4 cut(s)	105, 215, 841, 896
BsoBI	CYCGRG	1 cut(s)	439
Bsp1286I	GDGCHC	3 cut(s)	112, 284, 401
Bsp143I	GATC	6 cut(s)	67, 298, 415, 443, 465, 490
BspACI	CCGC	4 cut(s)	102, 118, 626, 638
BspANI	GGCC	4 cut(s)	105, 215, 841, 896
BspCNI	CTCAG	2 cut(s)	693, 844
BspDI	ATCGAT	1 cut(s)	414
BspLI	GGNNCC	2 cut(s)	436, 510
BspMI	ACCTGC	1 cut(s)	871
BspPI	GGATC	3 cut(s)	75, 438, 485
BspQI	GCTCTTC	3 cut(s)	193, 389, 586
BsrDI	GCAATG	1 cut(s)	174
BsrI	ACTGG	2 cut(s)	781, 807
BssECI	CCNNGG	1 cut(s)	210
BssMI	GATC	6 cut(s)	67, 298, 415, 443, 465, 490
BssT1I	CCWWGG	1 cut(s)	210
Bst2UI	CCWGG	2 cut(s)	432, 650
Bst4CI	ACNGT	3 cut(s)	463, 772, 847
Bst6I	CTCTTC	6 cut(s)	193, 299, 305, 389, 574, 586
BstAPI	GCANNNNNTGC	1 cut(s)	863
BstDEI	CTNAG	4 cut(s)	263, 374, 701, 831
BstF5I	GGATG	4 cut(s)	32, 77, 132, 349
BstKTI	GATC	6 cut(s)	70, 301, 418, 446, 468, 493
BstMAI	GTCTC	1 cut(s)	817
BstMBI	GATC	6 cut(s)	67, 298, 415, 443, 465, 490
BstMCI	CGRYCG	2 cut(s)	106, 118
BstMWI	GCNNNNNNNGC	3 cut(s)	38, 47, 863
BstNI	CCWGG	2 cut(s)	432, 650
BstSCI	CCNGG	2 cut(s)	430, 648
BstV1I	GCAGC	1 cut(s)	767
BstX2I	RGATCY	1 cut(s)	443
BstYI	RGATCY	1 cut(s)	443
BstZI	CGGCCG	1 cut(s)	103
Bsu15I	ATCGAT	1 cut(s)	414
BsuRI	GGCC	4 cut(s)	105, 215, 841, 896
BsuTUI	ATCGAT	1 cut(s)	414
BtsCI	GGATG	4 cut(s)	32, 77, 132, 349
BtsI	GCAGTG	3 cut(s)	540, 629, 676
BtsIMutI	CAGTG	5 cut(s)	540, 629, 676, 774, 814
BveI	ACCTGC	1 cut(s)	871
CaiI	CAGNNNCTG	1 cut(s)	776
ClaI	ATCGAT	1 cut(s)	414
Csp6I	GTAC	3 cut(s)	92, 273, 315
CviAII	CATG	4 cut(s)	58, 64, 183, 332
CviQI	GTAC	3 cut(s)	92, 273, 315
DdeI	CTNAG	4 cut(s)	263, 374, 701, 831
DpnI	GATC	6 cut(s)	69, 300, 417, 445, 467, 492
DpnII	GATC	6 cut(s)	67, 298, 415, 443, 465, 490
EaeI	YGGCCR	1 cut(s)	103
EagI	CGGCCG	1 cut(s)	103
Eam1104I	CTCTTC	6 cut(s)	193, 299, 305, 389, 574, 586
EarI	CTCTTC	6 cut(s)	193, 299, 305, 389, 574, 586
Ecl136II	GAGCTC	2 cut(s)	282, 399
EclXI	CGGCCG	1 cut(s)	103
Eco130I	CCWWGG	1 cut(s)	210
Eco147I	AGGCCT	1 cut(s)	215
Eco24I	GRGCYC	3 cut(s)	112, 284, 401
Eco52I	CGGCCG	1 cut(s)	103
Eco53kI	GAGCTC	2 cut(s)	282, 399
Eco88I	CYCGRG	1 cut(s)	439
EcoICRI	GAGCTC	2 cut(s)	282, 399
EcoRII	CCWGG	2 cut(s)	430, 648
EcoT14I	CCWWGG	1 cut(s)	210
EcoT22I	ATGCAT	1 cut(s)	59
EcoT38I	GRGCYC	3 cut(s)	112, 284, 401
ErhI	CCWWGG	1 cut(s)	210
FaeI	CATG	4 cut(s)	61, 67, 186, 335
FaiI	YATR	9 cut(s)	17, 23, 53, 55, 59, 65, 184, 333, 746
FaqI	GGGAC	1 cut(s)	655
FatI	CATG	4 cut(s)	57, 63, 182, 331
FauNDI	CATATG	1 cut(s)	53
FbaI	TGATCA	1 cut(s)	298
Fnu4HI	GCNGC	2 cut(s)	103, 756
FokI	GGATG	4 cut(s)	19, 64, 119, 356
FriOI	GRGCYC	3 cut(s)	112, 284, 401
Fsp4HI	GCNGC	2 cut(s)	103, 756
FspBI	CTAG	2 cut(s)	318, 890
GluI	GCNGC	2 cut(s)	103, 756
GsuI	CTGGAG	2 cut(s)	145, 798
HaeIII	GGCC	4 cut(s)	105, 215, 841, 896
HapII	CCGG	2 cut(s)	494, 512
Hin1II	CATG	4 cut(s)	61, 67, 186, 335
HindIII	AAGCTT	1 cut(s)	39
HpaII	CCGG	2 cut(s)	494, 512
HphI	GGTGA	4 cut(s)	742, 778, 832, 852
Hpy188I	TCNGA	3 cut(s)	303, 484, 597
Hpy188III	TCNNGA	3 cut(s)	162, 441, 717
HpyAV	CCTTC	5 cut(s)	179, 651, 683, 719, 757
HpyCH4III	ACNGT	3 cut(s)	463, 772, 847
HpyCH4V	TGCA	5 cut(s)	32, 57, 143, 533, 681
HpyF10VI	GCNNNNNNNGC	3 cut(s)	38, 47, 863
HpyF3I	CTNAG	4 cut(s)	263, 374, 701, 831
Hsp92II	CATG	4 cut(s)	61, 67, 186, 335
Ksp22I	TGATCA	1 cut(s)	298
Kzo9I	GATC	6 cut(s)	67, 298, 415, 443, 465, 490
LguI	GCTCTTC	3 cut(s)	193, 389, 586
LmnI	GCTCC	4 cut(s)	164, 184, 287, 440
Lsp1109I	GCAGC	1 cut(s)	767
LweI	GCATC	4 cut(s)	41, 86, 130, 866
MaeI	CTAG	2 cut(s)	318, 890
MaeIII	GTNAC	5 cut(s)	258, 730, 766, 772, 784
MalI	GATC	6 cut(s)	69, 300, 417, 445, 467, 492
MboI	GATC	6 cut(s)	67, 298, 415, 443, 465, 490
MfeI	CAATTG	1 cut(s)	573
MflI	RGATCY	1 cut(s)	443
MhlI	GDGCHC	3 cut(s)	112, 284, 401
MluCI	AATT	1 cut(s)	573
MmeI	TCCRAC	1 cut(s)	321
Mph1103I	ATGCAT	1 cut(s)	59
MseI	TTAA	1 cut(s)	354
MslI	CAYNNNNRTG	2 cut(s)	56, 62
MspI	CCGG	2 cut(s)	494, 512
MspR9I	CCNGG	2 cut(s)	432, 650
MunI	CAATTG	1 cut(s)	573
Mva1269I	GAATGC	1 cut(s)	535
MvaI	CCWGG	2 cut(s)	432, 650
MwoI	GCNNNNNNNGC	3 cut(s)	38, 47, 863
NdeI	CATATG	1 cut(s)	53
NdeII	GATC	6 cut(s)	67, 298, 415, 443, 465, 490
NlaIII	CATG	4 cut(s)	61, 67, 186, 335
NlaIV	GGNNCC	2 cut(s)	436, 510
NmeAIII	GCCGAG	2 cut(s)	78, 131
NmuCI	GTSAC	4 cut(s)	258, 730, 766, 772
NsiI	ATGCAT	1 cut(s)	59
PaeR7I	CTCGAG	1 cut(s)	439
PaqCI	CACCTGC	1 cut(s)	871
PceI	AGGCCT	1 cut(s)	215
PciSI	GCTCTTC	3 cut(s)	193, 389, 586
PctI	GAATGC	1 cut(s)	535
PkrI	GCNGC	2 cut(s)	104, 757
Psp124BI	GAGCTC	2 cut(s)	284, 401
Psp6I	CCWGG	2 cut(s)	430, 648
PspGI	CCWGG	2 cut(s)	430, 648
PspN4I	GGNNCC	2 cut(s)	436, 510
PstNI	CAGNNNCTG	1 cut(s)	776
PsuI	RGATCY	1 cut(s)	443
RsaI	GTAC	3 cut(s)	93, 274, 316
RsaNI	GTAC	3 cut(s)	92, 273, 315
RseI	CAYNNNNRTG	2 cut(s)	56, 62
SacI	GAGCTC	2 cut(s)	284, 401
SapI	GCTCTTC	3 cut(s)	193, 389, 586
SaqAI	TTAA	1 cut(s)	354
SatI	GCNGC	2 cut(s)	103, 756
Sau3AI	GATC	6 cut(s)	67, 298, 415, 443, 465, 490
ScaI	AGTACT	1 cut(s)	316
ScrFI	CCNGG	2 cut(s)	432, 650
SduI	GDGCHC	3 cut(s)	112, 284, 401
SfaNI	GCATC	4 cut(s)	41, 86, 130, 866
Sfr274I	CTCGAG	1 cut(s)	439
SlaI	CTCGAG	1 cut(s)	439
SmiMI	CAYNNNNRTG	2 cut(s)	56, 62
SmlI	CTYRAG	1 cut(s)	439
SmoI	CTYRAG	1 cut(s)	439
SpeI	ACTAGT	1 cut(s)	317
Sse9I	AATT	1 cut(s)	573
SseBI	AGGCCT	1 cut(s)	215
SsiI	CCGC	4 cut(s)	102, 118, 626, 638
SspI	AATATT	1 cut(s)	325
SspMI	CTAG	2 cut(s)	318, 890
SstI	GAGCTC	2 cut(s)	284, 401
StuI	AGGCCT	1 cut(s)	215
StyD4I	CCNGG	2 cut(s)	430, 648
StyI	CCWWGG	1 cut(s)	210
TaaI	ACNGT	3 cut(s)	463, 772, 847
TaqI	TCGA	3 cut(s)	114, 414, 440
TasI	AATT	1 cut(s)	573
TatI	WGTACW	1 cut(s)	314
TauI	GCSGC	1 cut(s)	105
Tru1I	TTAA	1 cut(s)	354
Tru9I	TTAA	1 cut(s)	354
TscAI	CASTG	5 cut(s)	540, 636, 683, 781, 814
TseFI	GTSAC	4 cut(s)	258, 730, 766, 772
TseI	GCWGC	1 cut(s)	755
Tsp45I	GTSAC	4 cut(s)	258, 730, 766, 772
TspDTI	ATGAA	5 cut(s)	17, 23, 74, 320, 360
TspRI	CASTG	5 cut(s)	540, 636, 683, 781, 814
XhoI	CTCGAG	1 cut(s)	439
XspI	CTAG	2 cut(s)	318, 890
ZrmI	AGTACT	1 cut(s)	316
Zsp2I	ATGCAT	1 cut(s)	59

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.