Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr04

Orthogroup ID

OG0010067

Physical Location & Seq

Genomic Coordinates Reverse (-)

20657977 .. 20660838

Gene Structure 2862 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

MD04G1120400.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1092 bp

ATGAGCATAGTCGAAACAATGAAGGCTGCCAAGCCTTTCTTTGCTGTTGTTTTCTTGCAATTTGGGCTTGCCGGCATGGATATTCTCTCTAAGGCAGCACTAAACCAGGGGATGAGTAACTACGTGCTCGTTGTCTACCGCCACGTTGTTGCCACTGCTGTCGTTGCTCCTTTCGCCGTCATACTGGACAAAAAAGTGAGACCGAAGATGACGCTTTCGATCTTCATCAAGATAATGCTGCTCGCTTTGCTGGAGCCAGTTCTGGACCAAAATCTGTACTTTATGGGAATGAAGTACACAACAGCAACGTTTGCAGCAGCTATGTGCAATATTCTTCCCGCCCTTACTTTTGTAATGGCTTGGGTTCTTAGGCTTGAGAAAGTGAATTTGAAAAGCATCCGGAGCCAAAGTAAGTTGGTTGGAACTGTAGCAACCGTTGCAGGTGCCATGATCATGACCCTCGTAAAGGGTCCACTTTTGCATTTATTTTGGACCAGAGCTAGCCATGAACAGCATACAAGTGCCGCTACTAGCCTCCAAGATTCGATCAAGGGTTCCCTAATGATCACAGTCGGCTGCTTCTGCTGGGCTTGTTTCATGATTCTGCAGGCAATCACACTGAAAACGTACCCAGCTGAGCTCTCCCTTTCGGCTTGGATATGTGCGTTGGGCACACTGGAAGGAACTGCTGTAGCATTGGTGATGGAAAGGGGAAATGCTGCCGTCTGGTCGATAAAATGGGATACAAAATTATTGGCAGCTAGTTACAGTGGAATATTCTGTTCAGGACTAGCTTATTACATTCAAGGAGTAGTAATGAAATACAGAGGCCCTGTGTTTGTGACAGCATTCAGTCCCCTGAGCATGGTTATTGTTGCTGTCATGAGCTCCTTCATCCTGCGTGAGCAAATGTTTCTTGGAAGGCTGCTGGGTGCTGTGGTCATAATCGCAGGCCTATATCTCGTTGTCTGGGGTAAAGCCAAAGATTACGAGTCCCCGGAAAAAACAATCGAAGATGAATTAACGGCAACGAAGCAAGCACAAGATGAAAAAGCTCTTGAGGCCATCACAATCGCACCATCTTGCAAATGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003333 GO:0003674 GO:0005215 GO:0005342 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005886 GO:0005887 GO:0005938 GO:0006810 GO:0006811 GO:0006820 GO:0006865 GO:0007154 GO:0007165 GO:0008150 GO:0008509 GO:0008514 GO:0009719 GO:0009725 GO:0009733 GO:0009734 GO:0009755 GO:0009987 GO:0010033 GO:0015075 GO:0015171 GO:0015318 GO:0015711 GO:0015849 GO:0016020 GO:0016021 GO:0022857 GO:0023052 GO:0031224 GO:0031226 GO:0032870 GO:0032973 GO:0034220 GO:0042221 GO:0042592 GO:0043090 GO:0044424 GO:0044425 GO:0044444 GO:0044459 GO:0044464 GO:0046942 GO:0046943 GO:0048878 GO:0050789 GO:0050794 GO:0050801 GO:0050896 GO:0051179 GO:0051234 GO:0051716 GO:0055081 GO:0055085 GO:0065007 GO:0065008 GO:0070887 GO:0071310 GO:0071365 GO:0071495 GO:0071702 GO:0071705 GO:0071944 GO:0080144 GO:0098656 GO:0099568 GO:0140115 GO:1903825 GO:1905039

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

EamA

Protein Analysis

364

Amino Acids

39.65

Weight (kDa)

9.2

Isoelectric Point (pI)

34.48

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
EamA	PF00892	20 - 153	1.2e-11	EamA-like transporter family
EamA	PF00892	184 - 323	2.3e-12	EamA-like transporter family

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

Style Settings

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0010067)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT2G37450 AT2G37450 AT2G37450 AT2G37460
fragaria_vesca	FvH4_6g14370 FvH4_6g14370 FvH4_6g14370 FvH4_6g14370 FvH4_6g14370
malus_domestica	MD04G1120400.v1.1
prunus_persica	Prupe.6G249600_v2.0.a1
pyrus_communis	pycom04g10950
rosa_chinensis	RchiOBHm_Chr3g0467431
rosa_laevigata	RLG00000024505
rosa_multiflora	Rmu_sc0001601.1_g000006
rosa_roxburghii	Rroxscaffold_6G00413680
rosa_rugosa	Rorug03G0086900
rosa_samantha	Rh3BG157000 Rh3CG156900 Rh3DG157800
rosa_wichuraiana	Rw3G012850

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AarI	CACCTGC	1 cut(s)	431
Acc36I	ACCTGC	1 cut(s)	431
AccB1I	GGYRCC	1 cut(s)	443
AccI	GTMKAC	1 cut(s)	135
AccIII	TCCGGA	1 cut(s)	399
AciI	CCGC	3 cut(s)	139, 339, 525
AclI	AACGTT	1 cut(s)	308
AcsI	RAATTY	1 cut(s)	385
AfaI	GTAC	3 cut(s)	278, 296, 629
AfiI	CCNNNNNNNGG	2 cut(s)	466, 865
AgsI	TTSAA	2 cut(s)	391, 806
AjnI	CCWGG	1 cut(s)	105
AluBI	AGCT	8 cut(s)	320, 500, 635, 640, 761, 794, 888, 1055
AluI	AGCT	8 cut(s)	320, 500, 635, 640, 761, 794, 888, 1055
Alw21I	GWGCWC	3 cut(s)	129, 642, 890
Alw26I	GTCTC	1 cut(s)	193
Aor13HI	TCCGGA	1 cut(s)	399
AoxI	GGCC	3 cut(s)	829, 952, 1062
ApeKI	GCWGC	9 cut(s)	26, 95, 238, 314, 317, 576, 719, 758, 925
ApoI	RAATTY	1 cut(s)	385
AspS9I	GGNCC	4 cut(s)	265, 470, 492, 830
AsuC2I	CCSGG	1 cut(s)	998
AsuHPI	GGTGA	1 cut(s)	712
AsuNHI	GCTAGC	1 cut(s)	500
AvaII	GGWCC	3 cut(s)	265, 470, 492
BaeGI	GKGCMC	1 cut(s)	674
BanI	GGYRCC	1 cut(s)	443
BanII	GRGCYC	2 cut(s)	642, 890
Bbv12I	GWGCWC	3 cut(s)	129, 642, 890
BbvI	GCAGC	9 cut(s)	13, 107, 225, 326, 329, 563, 706, 770, 912
BccI	CCATC	3 cut(s)	697, 1073, 1087
BceAI	ACGGC	3 cut(s)	161, 707, 1041
BciT130I	CCWGG	1 cut(s)	107
BciVI	GTATCC	1 cut(s)	736
BclI	TGATCA	2 cut(s)	450, 564
BcnI	CCSGG	1 cut(s)	998
BcoDI	GTCTC	1 cut(s)	193
BfaI	CTAG	4 cut(s)	501, 531, 762, 791
BfmI	CTRYAG	3 cut(s)	426, 605, 690
BfuAI	ACCTGC	1 cut(s)	431
BfuI	GTATCC	1 cut(s)	736
BlpI	GCTNAGC	1 cut(s)	636
Bme1390I	CCNGG	2 cut(s)	107, 998
Bme18I	GGWCC	3 cut(s)	265, 470, 492
BmgT120I	GGNCC	4 cut(s)	265, 470, 492, 830
BmiI	GGNNCC	5 cut(s)	255, 404, 445, 471, 556
BmrFI	CCNGG	2 cut(s)	107, 998
BmsI	GCATC	1 cut(s)	405
BmtI	GCTAGC	1 cut(s)	504
BpmI	CTGGAG	1 cut(s)	272
Bpu10I	CCTNAGC	1 cut(s)	860
Bpu1102I	GCTNAGC	1 cut(s)	636
BpuEI	CTTGAG	2 cut(s)	395, 1079
BpuMI	CCSGG	1 cut(s)	998
BsaAI	YACGTR	1 cut(s)	124
BsaBI	GATNNNNATC	1 cut(s)	224
BsaI	GGTCTC	1 cut(s)	193
BsaJI	CCNNGG	2 cut(s)	106, 996
BsaWI	WCCGGW	1 cut(s)	399
BsaXI	ACNNNNNCTCC	2 cut(s)	394, 424
Bsc4I	CCNNNNNNNGG	2 cut(s)	466, 865
Bse118I	RCCGGY	1 cut(s)	71
Bse1I	ACTGG	3 cut(s)	189, 257, 681
Bse8I	GATNNNNATC	1 cut(s)	224
BseAI	TCCGGA	1 cut(s)	399
BseBI	CCWGG	1 cut(s)	107
BseDI	CCNNGG	2 cut(s)	106, 996
BseGI	GGATG	3 cut(s)	117, 396, 894
BseJI	GATNNNNATC	1 cut(s)	224
BseLI	CCNNNNNNNGG	2 cut(s)	466, 865
BseMII	CTCAG	2 cut(s)	627, 851
BseNI	ACTGG	3 cut(s)	189, 257, 681
BseSI	GKGCMC	1 cut(s)	674
BseXI	GCAGC	9 cut(s)	13, 107, 225, 326, 329, 563, 706, 770, 912
BseYI	CCCAGC	3 cut(s)	585, 631, 928
BshFI	GGCC	3 cut(s)	831, 954, 1064
BshNI	GGYRCC	1 cut(s)	443
BsiHKAI	GWGCWC	3 cut(s)	129, 642, 890
BsiSI	CCGG	3 cut(s)	72, 400, 998
BslFI	GGGAC	2 cut(s)	840, 979
BslI	CCNNNNNNNGG	2 cut(s)	466, 865
BsmAI	GTCTC	1 cut(s)	193
BsmFI	GGGAC	2 cut(s)	840, 979
BsmI	GAATGC	1 cut(s)	848
BsnI	GGCC	3 cut(s)	831, 954, 1064
Bso31I	GGTCTC	1 cut(s)	193
Bsp1286I	GDGCHC	4 cut(s)	129, 642, 674, 890
Bsp13I	TCCGGA	1 cut(s)	399
Bsp143I	GATC	4 cut(s)	219, 450, 546, 564
Bsp1720I	GCTNAGC	1 cut(s)	636
BspACI	CCGC	3 cut(s)	139, 339, 525
BspANI	GGCC	3 cut(s)	831, 954, 1064
BspCNI	CTCAG	2 cut(s)	628, 852
BspEI	TCCGGA	1 cut(s)	399
BspHI	TCATGA	3 cut(s)	453, 597, 882
BspLI	GGNNCC	5 cut(s)	255, 404, 445, 471, 556
BspMAI	CTGCAG	1 cut(s)	609
BspMI	ACCTGC	1 cut(s)	431
BspOI	GCTAGC	1 cut(s)	504
BspT107I	GGYRCC	1 cut(s)	443
BspTNI	GGTCTC	1 cut(s)	193
BsrFI	RCCGGY	1 cut(s)	71
BsrI	ACTGG	3 cut(s)	189, 257, 681
BssAI	RCCGGY	1 cut(s)	71
BssECI	CCNNGG	2 cut(s)	106, 996
BssMI	GATC	4 cut(s)	219, 450, 546, 564
Bst2UI	CCWGG	1 cut(s)	107
Bst4CI	ACNGT	4 cut(s)	427, 436, 571, 770
BstAPI	GCANNNNNTGC	2 cut(s)	311, 437
BstBAI	YACGTR	1 cut(s)	124
BstC8I	GCNNGC	7 cut(s)	69, 73, 243, 502, 609, 952, 1038
BstDEI	CTNAG	4 cut(s)	90, 368, 636, 860
BstENI	CCTNNNNNAGG	1 cut(s)	464
BstF5I	GGATG	3 cut(s)	117, 396, 894
BstKTI	GATC	4 cut(s)	222, 453, 549, 567
BstMAI	GTCTC	1 cut(s)	193
BstMBI	GATC	4 cut(s)	219, 450, 546, 564
BstMWI	GCNNNNNNNGC	9 cut(s)	64, 164, 173, 247, 311, 402, 437, 582, 1061
BstNI	CCWGG	1 cut(s)	107
BstSCI	CCNGG	2 cut(s)	105, 996
BstSFI	CTRYAG	3 cut(s)	426, 605, 690
BstSLI	GKGCMC	1 cut(s)	674
BstV1I	GCAGC	9 cut(s)	13, 107, 225, 326, 329, 563, 706, 770, 912
BsuI	GTATCC	1 cut(s)	736
BsuRI	GGCC	3 cut(s)	831, 954, 1064
BtsCI	GGATG	3 cut(s)	117, 396, 894
BtsI	GCAGTG	1 cut(s)	153
BtsIMutI	CAGTG	4 cut(s)	153, 617, 674, 775
BveI	ACCTGC	1 cut(s)	431
Cac8I	GCNNGC	7 cut(s)	69, 73, 243, 502, 609, 952, 1038
CciI	TCATGA	3 cut(s)	453, 597, 882
Cfr10I	RCCGGY	1 cut(s)	71
Cfr13I	GGNCC	4 cut(s)	265, 470, 492, 830
CseI	GACGC	1 cut(s)	220
Csp6I	GTAC	3 cut(s)	277, 295, 628
CviAII	CATG	7 cut(s)	76, 448, 454, 506, 598, 865, 883
CviQI	GTAC	3 cut(s)	277, 295, 628
DdeI	CTNAG	4 cut(s)	90, 368, 636, 860
DpnI	GATC	4 cut(s)	221, 452, 548, 566
DpnII	GATC	4 cut(s)	219, 450, 546, 564
Ecl136II	GAGCTC	2 cut(s)	640, 888
Eco147I	AGGCCT	1 cut(s)	954
Eco24I	GRGCYC	2 cut(s)	642, 890
Eco31I	GGTCTC	1 cut(s)	193
Eco47I	GGWCC	3 cut(s)	265, 470, 492
Eco53kI	GAGCTC	2 cut(s)	640, 888
EcoICRI	GAGCTC	2 cut(s)	640, 888
EcoNI	CCTNNNNNAGG	1 cut(s)	464
EcoO109I	RGGNCCY	1 cut(s)	830
EcoRII	CCWGG	1 cut(s)	105
EcoT38I	GRGCYC	2 cut(s)	642, 890
FaeI	CATG	7 cut(s)	79, 451, 457, 509, 601, 868, 886
FalI	AAGNNNNNCTT	2 cut(s)	23, 55
FaqI	GGGAC	2 cut(s)	840, 979
FatI	CATG	7 cut(s)	75, 447, 453, 505, 597, 864, 882
FauI	CCCGC	1 cut(s)	346
FbaI	TGATCA	2 cut(s)	450, 564
FblI	GTMKAC	1 cut(s)	135
FokI	GGATG	3 cut(s)	124, 383, 881
FriOI	GRGCYC	2 cut(s)	642, 890
FspBI	CTAG	4 cut(s)	501, 531, 762, 791
GsaI	CCCAGC	3 cut(s)	589, 635, 932
GsuI	CTGGAG	1 cut(s)	272
HaeIII	GGCC	3 cut(s)	831, 954, 1064
HapII	CCGG	3 cut(s)	72, 400, 998
HgaI	GACGC	1 cut(s)	220
Hin1II	CATG	7 cut(s)	79, 451, 457, 509, 601, 868, 886
HinfI	GANTC	3 cut(s)	542, 601, 992
HpaII	CCGG	3 cut(s)	72, 400, 998
HphI	GGTGA	1 cut(s)	712
Hpy166II	GTNNAC	3 cut(s)	136, 297, 473
Hpy188III	TCNNGA	8 cut(s)	229, 263, 400, 454, 598, 786, 883, 1058
Hpy8I	GTNNAC	3 cut(s)	136, 297, 473
HpyAV	CCTTC	4 cut(s)	16, 674, 901, 915
HpyCH4III	ACNGT	4 cut(s)	427, 436, 571, 770
HpyCH4IV	ACGT	4 cut(s)	123, 144, 308, 626
HpyCH4V	TGCA	7 cut(s)	58, 314, 327, 440, 481, 607, 1086
HpyF10VI	GCNNNNNNNGC	9 cut(s)	64, 164, 173, 247, 311, 402, 437, 582, 1061
HpyF3I	CTNAG	4 cut(s)	90, 368, 636, 860
HpySE526I	ACGT	4 cut(s)	123, 144, 308, 626
Hsp92II	CATG	7 cut(s)	79, 451, 457, 509, 601, 868, 886
Kpn2I	TCCGGA	1 cut(s)	399
KroI	GCCGGC	1 cut(s)	71
KroNI	GCCGGC	1 cut(s)	73
Ksp22I	TGATCA	2 cut(s)	450, 564
Kzo9I	GATC	4 cut(s)	219, 450, 546, 564
LmnI	GCTCC	4 cut(s)	172, 253, 402, 893
Lsp1109I	GCAGC	9 cut(s)	13, 107, 225, 326, 329, 563, 706, 770, 912
LweI	GCATC	1 cut(s)	405
MaeI	CTAG	4 cut(s)	501, 531, 762, 791
MaeII	ACGT	4 cut(s)	123, 144, 308, 626
MaeIII	GTNAC	3 cut(s)	116, 764, 841
MalI	GATC	4 cut(s)	221, 452, 548, 566
MboI	GATC	4 cut(s)	219, 450, 546, 564
MboII	GAAGA	4 cut(s)	214, 217, 326, 1025
MhlI	GDGCHC	4 cut(s)	129, 642, 674, 890
MluCI	AATT	4 cut(s)	59, 385, 749, 1019
MlyI	GAGTC	1 cut(s)	1001
MmeI	TCCRAC	1 cut(s)	400
MnlI	CCTC	4 cut(s)	470, 545, 821, 1054
MroI	TCCGGA	1 cut(s)	399
MroNI	GCCGGC	1 cut(s)	71
MseI	TTAA	1 cut(s)	1022
MslI	CAYNNNNRTG	2 cut(s)	452, 519
MspA1I	CMGCKG	1 cut(s)	635
MspI	CCGG	3 cut(s)	72, 400, 998
MspR9I	CCNGG	2 cut(s)	107, 998
Mva1269I	GAATGC	1 cut(s)	848
MvaI	CCWGG	1 cut(s)	107
MwoI	GCNNNNNNNGC	9 cut(s)	64, 164, 173, 247, 311, 402, 437, 582, 1061
NaeI	GCCGGC	1 cut(s)	73
NciI	CCSGG	1 cut(s)	998
NdeII	GATC	4 cut(s)	219, 450, 546, 564
NgoMIV	GCCGGC	1 cut(s)	71
NheI	GCTAGC	1 cut(s)	500
NlaIII	CATG	7 cut(s)	79, 451, 457, 509, 601, 868, 886
NlaIV	GGNNCC	5 cut(s)	255, 404, 445, 471, 556
NmuCI	GTSAC	1 cut(s)	841
PagI	TCATGA	3 cut(s)	453, 597, 882
PaqCI	CACCTGC	1 cut(s)	431
PceI	AGGCCT	1 cut(s)	954
PctI	GAATGC	1 cut(s)	848
PdiI	GCCGGC	1 cut(s)	73
PfeI	GAWTC	2 cut(s)	542, 601
PleI	GAGTC	1 cut(s)	1000
PpsI	GAGTC	1 cut(s)	1000
Ppu21I	YACGTR	1 cut(s)	124
Psp124BI	GAGCTC	2 cut(s)	642, 890
Psp1406I	AACGTT	1 cut(s)	308
Psp6I	CCWGG	1 cut(s)	105
PspFI	CCCAGC	3 cut(s)	585, 631, 928
PspGI	CCWGG	1 cut(s)	105
PspN4I	GGNNCC	5 cut(s)	255, 404, 445, 471, 556
PspPI	GGNCC	4 cut(s)	265, 470, 492, 830
PstI	CTGCAG	1 cut(s)	609
PvuII	CAGCTG	1 cut(s)	635
RsaI	GTAC	3 cut(s)	278, 296, 629
RsaNI	GTAC	3 cut(s)	277, 295, 628
RseI	CAYNNNNRTG	2 cut(s)	452, 519
SacI	GAGCTC	2 cut(s)	642, 890
SaqAI	TTAA	1 cut(s)	1022
Sau3AI	GATC	4 cut(s)	219, 450, 546, 564
Sau96I	GGNCC	4 cut(s)	265, 470, 492, 830
SchI	GAGTC	1 cut(s)	1001
ScrFI	CCNGG	2 cut(s)	107, 998
SduI	GDGCHC	4 cut(s)	129, 642, 674, 890
SfaNI	GCATC	1 cut(s)	405
SfcI	CTRYAG	3 cut(s)	426, 605, 690
SinI	GGWCC	3 cut(s)	265, 470, 492
SmiMI	CAYNNNNRTG	2 cut(s)	452, 519
SmlI	CTYRAG	2 cut(s)	374, 1058
SmoI	CTYRAG	2 cut(s)	374, 1058
Sse9I	AATT	4 cut(s)	59, 385, 749, 1019
SseBI	AGGCCT	1 cut(s)	954
SsiI	CCGC	3 cut(s)	139, 339, 525
SspI	AATATT	2 cut(s)	331, 777
SspMI	CTAG	4 cut(s)	501, 531, 762, 791
SstI	GAGCTC	2 cut(s)	642, 890
StuI	AGGCCT	1 cut(s)	954
StyD4I	CCNGG	2 cut(s)	105, 996
TaaI	ACNGT	4 cut(s)	427, 436, 571, 770
TaiI	ACGT	4 cut(s)	126, 147, 311, 629
TaqI	TCGA	5 cut(s)	12, 218, 545, 731, 1011
TaqII	GACCGA	1 cut(s)	217
TasI	AATT	4 cut(s)	59, 385, 749, 1019
TatI	WGTACW	2 cut(s)	276, 294
TauI	GCSGC	1 cut(s)	527
TfiI	GAWTC	2 cut(s)	542, 601
Tru1I	TTAA	1 cut(s)	1022
Tru9I	TTAA	1 cut(s)	1022
TscAI	CASTG	4 cut(s)	160, 624, 681, 775
TseFI	GTSAC	1 cut(s)	841
TseI	GCWGC	9 cut(s)	26, 95, 238, 314, 317, 576, 719, 758, 925
Tsp45I	GTSAC	1 cut(s)	841
TspDTI	ATGAA	9 cut(s)	35, 214, 305, 522, 586, 833, 883, 1032, 1062
TspRI	CASTG	4 cut(s)	160, 624, 681, 775
VpaK11BI	GGWCC	3 cut(s)	265, 470, 492
XagI	CCTNNNNNAGG	1 cut(s)	464
XapI	RAATTY	1 cut(s)	385
XmiI	GTMKAC	1 cut(s)	135
XspI	CTAG	4 cut(s)	501, 531, 762, 791

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.