Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr04

Orthogroup ID

OG0003101

Physical Location & Seq

Genomic Coordinates Forward (+)

20986348 .. 20989604

Gene Structure 3257 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

MD04G1123800.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 2442 bp

ATGGTTTCGGCCACCAGGATGGGCTGGACCTATGCCGTTTGGTGCCTGATTCTACGAGCAGCGTTTCGAACCATCTTAGCCTGCACCATAGTCTACACCACCACCCTCTACGGTCCACAACCTCTCAGGCGTTTAGTAGCCTACCCTGCATACTCTTACCTCACCACAATCCTCATCGTCTCAGAAGCGACGCTGGGGGAGGCACTGAGAGGCTTCTGGCATGCGCTTTACGCTTGTATTCAGGTGTTGATCCTTTCAATCCTGAGCCTCCGGCTAATAGGGCCAGCGCACTTCAGTTACTTTGTATCATCCCTTGCAGTGGCTTTGTGCGCTTTTGTTGTGGCACTGCCGGGAGCAACCCACTTGATGTCTAAGCGGATTGCGTTCGGGCAGATTGTGATTGTGTATGTAGGCACTGCCATTCATTGTGCAGAAGCTGATGAGTTGGTACTTATGCACCCGATTCAGGTGTCGTTGAGTACTATGCTTGGAGCCTCGGCATCTGTTGGGGCTATGTTGTTTCCCAACCTGTTTTTCTATCAGAAGGCATCTCTGGCGGCTGTGTTGTCTCCCTACCTTGCCTACGACGAGGTGAGACAAATGTGCCAAAAATATGCCGAGAATGCTAGGAAAAGGTTCGACTTGTACTTAGATGCAATCTTGTCTCAAGACAGCTCTGCTGCACTTGACTTTATTTTACAGGCCAAGCCCTTGGCTGAAGTAGGAGAAAAGCTCCTTCGAAGTATTGAACATAAACAGGAAGCCTTACTGTGGGAGAGACCAACAATCAGAAATATCCTCAACCGTAGTCGCTTCTCTGTTCGAGAAAAATTGCAGGAAGTTGAAATACCCTTGAGAGGGATTGCAATAGCTGCAACTTCATGCCCTTCCTTTCCTGTTGCCATGATTAAGAAACTGCAAGCGGACGCTTTACTCGACCTGAAAAAGCGAATACATGACAAGCTTGAGCAACTTGAGCACTTTGCACCGTTCTACACAAGAAAGAAAAAGGAAAGATCTTTGGATGAGGCCTTCTGGTCCCTTGAAACCATTTCTCATGAGGATTTATTGGCCTTCTTTTTCATTGATTGTCTGAAAATTCTCCAAGAAAATACCGAGGCTGTTGAAGAATGCAAGCATGAGTGCTCTGGCATCGAAAGCTCAAGCGGTTCACAAAATCAAAGCAATTGTTGTGCCAAATGGAGCTGTCGACTTTTGTGCATTATACCAAGCAAAACAAGTCTACGATTTGCAGTTAAGTGTTCACTTTCATTAGGCCTTGCAGTGCAGCTGGGTTTCCTGTTTAACAAGAGAGAAGCATATTGGGCAGGACTCACAATTGCAATAAGTTTTGTCAGGGGAAGACAAGCAACATTCACTCTCGCAAATGCTCGAGCGCAATCTACGGCAATGGGGTCAATCTACGGGATCTTGTGTTGCTTCATATTCAAGAAAGTTGAGGACTTGAGCTTCTTACCTCTTCTACCTTGGTTAGTTTTGACAAGCTTTCTGAGGCACAGCAGGATGTATGGCCAAGCTGGTGGAATATCAGCAGCTGTAGGGGCACTACTAATACTCGGCAGGAGACATTATGGCCCTCCACTCGATTTTGCGATCGCTAGAATCACAGAGGCTGTCATTGGATTGATCTGTTTCATCACCGTAGAGATTCTCACATCCCCTGTAAGAGCAGCAACTCTGGCAAAACTCAAACTTTCACTTGGACTGGGATCCCTTCAAGAATGTATCAAAGATTTAGTTCTTTTCGACAACAAAATCGCTTCAGTGGGTCCAAAGTTGAGACAGAAACAGAACAAGTTGAAATCCCACGTCAACAAGTTAGAGAAGTTCATTGAAGAAGCTCAGCTGGAGCCTAATTTCTGGTTCATCCCTTTCAACGGTGCCTGTTATGACAAGCTCCTGAGATCTTTATCTAACACGGGGAATCTTTCCATTATCATGTCCAACATAATAGAATCTCTATCATTCGTGTTACAGTGCTCCGAACTTGATTCCGAAGAACTCCGAAAACCCATGAAGGGGATCGAGAAAGATTTGGAGATTTTCAAGAAAAAAATTGGGAATTCTCTGCAATGCCTCCAAGAGCTGGCTTCCAATAAGTCTCTTGCAGTCCTTGGCAAACATGACATTGAGTCGGGAACATTATCAGCAAGAGAAAATGGTTCTAGGGTTTCGGATTCAGACAAGGAAGAGACTGAATGCCTTGTAGATTCTTTTCTTCAAAATTCCAATGAATTATCCGACAAAATTGCCACCATGACTGGATTAATAGAGGATCATCAGAAGCTTAGAGGGCAAGTAACTGTTTGTTTGGGTGGATTAGGGTTTTGCATCAGCAGTCTGCTGGGCCTGGGGGAATTAAAAGAGATGGAGAAAGATTTTCAAGAACTTATCAAATTGGAACACCCTTCAAGCATGTAA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

No ontology terms assigned.

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

FUSC FUSC_2

Protein Analysis

814

Amino Acids

90.4

Weight (kDa)

8.43

Isoelectric Point (pI)

52.23

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
FUSC	PF04632	415 - 568	2.3e-07	Fusaric acid resistance protein family
FUSC_2	PF13515	428 - 554	4.2e-10	Fusaric acid resistance protein-like

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0003101)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT3G09450
fragaria_vesca	FvH4_6g14110 FvH4_6g14110 FvH4_6g14240
malus_domestica	MD04G1122800.v1.1 MD04G1123800.v1.1 MD04G1125300.v1.1 MD12G1139000.v1.1 MD12G1140100.v1.1 MD12G1140200.v1.1 MD12G1140300.v1.1
prunus_persica	Prupe.6G250900_v2.0.a1 Prupe.6G250900_v2.0.a1 Prupe.6G250900_v2.0.a1 Prupe.6G252300_v2.0.a1
pyrus_communis	pycom04g11100 pycom04g11170 pycom12g13330
rosa_chinensis	RchiOBHm_Chr3g0467011 RchiOBHm_Chr3g0467201
rosa_laevigata	RLG00000024520 RLG00000024536
rosa_multiflora	Rmu_sc0001193.1_g000008
rosa_roxburghii	Rroxscaffold_6G00413830 Rroxscaffold_6G00414010
rosa_rugosa	Rorug03G0084800 Rorug03G0085700
rosa_samantha	Rh3AG132700 Rh3AG134100 Rh3BG153700 Rh3BG155400 Rh3CG153700 Rh3CG155000 Rh3DG154800 Rh3DG156200
rosa_wichuraiana	Rw3G012570 Rw3G012690

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AccB1I	GGYRCC	2 cut(s)	42, 1901
AccB7I	CCANNNNNTGG	1 cut(s)	2107
AccI	GTMKAC	3 cut(s)	93, 1210, 1243
AciI	CCGC	4 cut(s)	376, 557, 923, 1167
AclWI	GGATC	6 cut(s)	244, 1436, 1725, 1738, 2051, 2304
AcoI	YGGCCR	2 cut(s)	9, 1531
AcsI	RAATTY	3 cut(s)	1098, 2083, 2245
AcuI	CTGAAG	3 cut(s)	277, 738, 1767
AfaI	GTAC	3 cut(s)	450, 481, 647
AfiI	CCNNNNNNNGG	9 cut(s)	319, 466, 771, 857, 858, 1898, 2039, 2040, 2107
AhdI	GACNNNNNGTC	1 cut(s)	2152
AjiI	CACGTC	1 cut(s)	1831
AjnI	CCWGG	2 cut(s)	14, 2370
AjuI	GAANNNNNNNTTGG	2 cut(s)	518, 550
Alw21I	GWGCWC	3 cut(s)	981, 1148, 2003
Alw26I	GTCTC	9 cut(s)	184, 573, 589, 669, 772, 1579, 1795, 2127, 2207
AlwI	GGATC	6 cut(s)	244, 1436, 1725, 1738, 2051, 2304
AlwNI	CAGNNNCTG	3 cut(s)	437, 1556, 1634
Ama87I	CYCGRG	1 cut(s)	1392
AoxI	GGCC	9 cut(s)	9, 281, 702, 1029, 1071, 1276, 1531, 1594, 2368
ApeKI	GCWGC	6 cut(s)	59, 680, 872, 1288, 1553, 1691
ApoI	RAATTY	3 cut(s)	1098, 2083, 2245
AseI	ATTAAT	1 cut(s)	2288
AsiSI	GCGATCGC	1 cut(s)	1617
Asp700I	GAANNNNTTC	1 cut(s)	635
AspLEI	GCGC	4 cut(s)	226, 289, 332, 1399
AspS9I	GGNCC	7 cut(s)	27, 113, 281, 1038, 1595, 1790, 2368
AsuC2I	CCSGG	1 cut(s)	351
AsuHPI	GGTGA	3 cut(s)	154, 604, 1651
AsuII	TTCGAA	2 cut(s)	67, 739
AvaI	CYCGRG	1 cut(s)	1392
AvaII	GGWCC	4 cut(s)	27, 113, 1038, 1790
BaeGI	GKGCMC	1 cut(s)	1567
BalI	TGGCCA	1 cut(s)	1533
BamHI	GGATCC	1 cut(s)	1730
BanI	GGYRCC	2 cut(s)	42, 1901
BarI	GAAGNNNNNNTAC	2 cut(s)	831, 863
BbsI	GAAGAC	1 cut(s)	1369
Bbv12I	GWGCWC	3 cut(s)	981, 1148, 2003
BbvI	GCAGC	6 cut(s)	71, 667, 859, 1300, 1565, 1703
BccI	CCATC	3 cut(s)	13, 80, 2383
BceAI	ACGGC	2 cut(s)	20, 1422
BcgI	CGANNNNNNTGC	4 cut(s)	1200, 1234, 2252, 2286
BciT130I	CCWGG	2 cut(s)	16, 2372
BcnI	CCSGG	1 cut(s)	351
BcoDI	GTCTC	9 cut(s)	184, 573, 589, 669, 772, 1579, 1795, 2127, 2207
BfaI	CTAG	3 cut(s)	627, 1620, 2187
BfmI	CTRYAG	1 cut(s)	1557
BglII	AGATCT	2 cut(s)	1016, 1925
BisI	GCNGC	7 cut(s)	60, 558, 681, 873, 1289, 1554, 1692
BlpI	GCTNAGC	1 cut(s)	1863
BlsI	GCNGC	7 cut(s)	61, 559, 682, 874, 1290, 1555, 1693
BmcAI	AGTACT	1 cut(s)	481
Bme1390I	CCNGG	3 cut(s)	16, 351, 2372
Bme18I	GGWCC	4 cut(s)	27, 113, 1038, 1790
BmeRI	GACNNNNNGTC	1 cut(s)	2152
BmeT110I	CYCGRG	1 cut(s)	1392
BmgBI	CACGTC	1 cut(s)	1831
BmgT120I	GGNCC	7 cut(s)	27, 113, 281, 1038, 1595, 1790, 2368
BmiI	GGNNCC	7 cut(s)	44, 493, 1040, 1732, 1791, 1872, 1903
BmrFI	CCNGG	3 cut(s)	16, 351, 2372
BmrI	ACTGGG	1 cut(s)	1736
BmsI	GCATC	5 cut(s)	509, 557, 643, 1161, 2361
BmuI	ACTGGG	1 cut(s)	1736
BpiI	GAAGAC	1 cut(s)	1369
BplI	GAGNNNNNCTC	2 cut(s)	717, 749
BpmI	CTGGAG	1 cut(s)	1889
Bpu10I	CCTNAGC	1 cut(s)	263
Bpu1102I	GCTNAGC	1 cut(s)	1863
Bpu14I	TTCGAA	2 cut(s)	67, 739
BpuEI	CTTGAG	6 cut(s)	651, 874, 986, 995, 1147, 1486
BpuMI	CCSGG	1 cut(s)	351
BsaBI	GATNNNNATC	1 cut(s)	1930
BsaI	GGTCTC	1 cut(s)	772
BsaJI	CCNNGG	6 cut(s)	495, 711, 1116, 1487, 2134, 2371
Bsc4I	CCNNNNNNNGG	9 cut(s)	319, 466, 771, 857, 858, 1898, 2039, 2040, 2107
Bse1I	ACTGG	2 cut(s)	1731, 2287
Bse3DI	GCAATG	2 cut(s)	1416, 2099
Bse8I	GATNNNNATC	1 cut(s)	1930
BseBI	CCWGG	2 cut(s)	16, 2372
BseDI	CCNNGG	6 cut(s)	495, 711, 1116, 1487, 2134, 2371
BseGI	GGATG	6 cut(s)	24, 308, 1030, 1530, 1676, 1887
BseJI	GATNNNNATC	1 cut(s)	1930
BseLI	CCNNNNNNNGG	9 cut(s)	319, 466, 771, 857, 858, 1898, 2039, 2040, 2107
BseMI	GCAATG	2 cut(s)	1416, 2099
BseMII	CTCAG	7 cut(s)	139, 195, 197, 254, 1502, 1877, 1913
BseNI	ACTGG	2 cut(s)	1731, 2287
BseSI	GKGCMC	1 cut(s)	1567
BseXI	GCAGC	6 cut(s)	71, 667, 859, 1300, 1565, 1703
BseYI	CCCAGC	3 cut(s)	193, 1291, 2365
BsgI	GTGCAG	4 cut(s)	67, 450, 666, 1307
Bsh1285I	CGRYCG	1 cut(s)	1617
BshFI	GGCC	9 cut(s)	11, 283, 704, 1031, 1073, 1278, 1533, 1596, 2370
BshNI	GGYRCC	2 cut(s)	42, 1901
BsiEI	CGRYCG	1 cut(s)	1617
BsiHKAI	GWGCWC	3 cut(s)	981, 1148, 2003
BsiHKCI	CYCGRG	1 cut(s)	1392
BsiSI	CCGG	2 cut(s)	271, 350
BslFI	GGGAC	1 cut(s)	1024
BslI	CCNNNNNNNGG	9 cut(s)	319, 466, 771, 857, 858, 1898, 2039, 2040, 2107
BsmAI	GTCTC	9 cut(s)	184, 573, 589, 669, 772, 1579, 1795, 2127, 2207
BsmBI	CGTCTC	1 cut(s)	184
BsmFI	GGGAC	1 cut(s)	1024
BsmI	GAATGC	3 cut(s)	628, 1136, 2225
BsnI	GGCC	9 cut(s)	11, 283, 704, 1031, 1073, 1278, 1533, 1596, 2370
Bso31I	GGTCTC	1 cut(s)	772
BsoBI	CYCGRG	1 cut(s)	1392
Bsp119I	TTCGAA	2 cut(s)	67, 739
Bsp1286I	GDGCHC	4 cut(s)	981, 1148, 1567, 2003
Bsp143I	GATC	9 cut(s)	249, 1016, 1428, 1614, 1647, 1730, 1925, 2043, 2296
Bsp1720I	GCTNAGC	1 cut(s)	1863
BspACI	CCGC	4 cut(s)	376, 557, 923, 1167
BspANI	GGCC	9 cut(s)	11, 283, 704, 1031, 1073, 1278, 1533, 1596, 2370
BspCNI	CTCAG	7 cut(s)	138, 194, 198, 255, 1503, 1876, 1914
BspHI	TCATGA	1 cut(s)	1057
BspLI	GGNNCC	7 cut(s)	44, 493, 1040, 1732, 1791, 1872, 1903
BspPI	GGATC	6 cut(s)	244, 1436, 1725, 1738, 2051, 2304
BspT104I	TTCGAA	2 cut(s)	67, 739
BspT107I	GGYRCC	2 cut(s)	42, 1901
BspTNI	GGTCTC	1 cut(s)	772
BsrDI	GCAATG	2 cut(s)	1416, 2099
BsrI	ACTGG	2 cut(s)	1731, 2287
BssECI	CCNNGG	6 cut(s)	495, 711, 1116, 1487, 2134, 2371
BssMI	GATC	9 cut(s)	249, 1016, 1428, 1614, 1647, 1730, 1925, 2043, 2296
BssT1I	CCWWGG	3 cut(s)	711, 1487, 2134
Bst2UI	CCWGG	2 cut(s)	16, 2372
Bst4CI	ACNGT	8 cut(s)	113, 771, 806, 990, 1663, 1901, 1998, 2326
Bst6I	CTCTTC	2 cut(s)	1485, 2205
BstAPI	GCANNNNNTGC	1 cut(s)	872
BstBI	TTCGAA	2 cut(s)	67, 739
BstC8I	GCNNGC	6 cut(s)	82, 222, 285, 921, 1136, 2109
BstF5I	GGATG	6 cut(s)	24, 308, 1030, 1530, 1676, 1887
BstHHI	GCGC	4 cut(s)	226, 289, 332, 1399
BstKTI	GATC	9 cut(s)	252, 1019, 1431, 1617, 1650, 1733, 1928, 2046, 2299
BstMAI	GTCTC	9 cut(s)	184, 573, 589, 669, 772, 1579, 1795, 2127, 2207
BstMBI	GATC	9 cut(s)	249, 1016, 1428, 1614, 1647, 1730, 1925, 2043, 2296
BstMCI	CGRYCG	1 cut(s)	1617
BstNI	CCWGG	2 cut(s)	16, 2372
BstNSI	RCATGY	2 cut(s)	224, 2440
BstSCI	CCNGG	3 cut(s)	14, 349, 2370
BstSFI	CTRYAG	1 cut(s)	1557
BstSLI	GKGCMC	1 cut(s)	1567
BstV1I	GCAGC	6 cut(s)	71, 667, 859, 1300, 1565, 1703
BstV2I	GAAGAC	1 cut(s)	1369
BstX2I	RGATCY	4 cut(s)	1016, 1428, 1730, 1925
BstXI	CCANNNNNNTGG	3 cut(s)	19, 712, 1541
BstYI	RGATCY	4 cut(s)	1016, 1428, 1730, 1925
BsuRI	GGCC	9 cut(s)	11, 283, 704, 1031, 1073, 1278, 1533, 1596, 2370
BtrI	CACGTC	1 cut(s)	1831
BtsCI	GGATG	6 cut(s)	24, 308, 1030, 1530, 1676, 1887
BtsI	GCAGTG	4 cut(s)	324, 344, 414, 1290
BtsIMutI	CAGTG	7 cut(s)	203, 324, 344, 414, 1290, 1791, 2003
Cac8I	GCNNGC	6 cut(s)	82, 222, 285, 921, 1136, 2109
CaiI	CAGNNNCTG	3 cut(s)	437, 1556, 1634
CciI	TCATGA	1 cut(s)	1057
CfoI	GCGC	4 cut(s)	226, 289, 332, 1399
Cfr13I	GGNCC	7 cut(s)	27, 113, 281, 1038, 1595, 1790, 2368
CseI	GACGC	2 cut(s)	199, 935
Csp6I	GTAC	3 cut(s)	449, 480, 646
CviQI	GTAC	3 cut(s)	449, 480, 646
DpnI	GATC	9 cut(s)	251, 1018, 1430, 1616, 1649, 1732, 1927, 2045, 2298
DpnII	GATC	9 cut(s)	249, 1016, 1428, 1614, 1647, 1730, 1925, 2043, 2296
DriI	GACNNNNNGTC	1 cut(s)	2152
EaeI	YGGCCR	2 cut(s)	9, 1531
Eam1104I	CTCTTC	2 cut(s)	1485, 2205
Eam1105I	GACNNNNNGTC	1 cut(s)	2152
EarI	CTCTTC	2 cut(s)	1485, 2205
Eco130I	CCWWGG	3 cut(s)	711, 1487, 2134
Eco147I	AGGCCT	2 cut(s)	1031, 1278
Eco31I	GGTCTC	1 cut(s)	772
Eco47I	GGWCC	4 cut(s)	27, 113, 1038, 1790
Eco57I	CTGAAG	3 cut(s)	277, 738, 1767
Eco88I	CYCGRG	1 cut(s)	1392
EcoRI	GAATTC	1 cut(s)	2083
EcoRII	CCWGG	2 cut(s)	14, 2370
EcoT14I	CCWWGG	3 cut(s)	711, 1487, 2134
ErhI	CCWWGG	3 cut(s)	711, 1487, 2134
Esp3I	CGTCTC	1 cut(s)	184
FaqI	GGGAC	1 cut(s)	1024
FblI	GTMKAC	3 cut(s)	93, 1210, 1243
Fnu4HI	GCNGC	7 cut(s)	60, 558, 681, 873, 1289, 1554, 1692
FokI	GGATG	6 cut(s)	31, 295, 1037, 1537, 1663, 1874
Fsp4HI	GCNGC	7 cut(s)	60, 558, 681, 873, 1289, 1554, 1692
FspBI	CTAG	3 cut(s)	627, 1620, 2187
GlaI	GCGC	4 cut(s)	225, 288, 331, 1398
GluI	GCNGC	7 cut(s)	60, 558, 681, 873, 1289, 1554, 1692
GsaI	CCCAGC	3 cut(s)	197, 1295, 2369
GsuI	CTGGAG	1 cut(s)	1889
HaeIII	GGCC	9 cut(s)	11, 283, 704, 1031, 1073, 1278, 1533, 1596, 2370
HapII	CCGG	2 cut(s)	271, 350
HgaI	GACGC	2 cut(s)	199, 935
HhaI	GCGC	4 cut(s)	226, 289, 332, 1399
Hin6I	GCGC	4 cut(s)	224, 287, 330, 1397
HinP1I	GCGC	4 cut(s)	224, 287, 330, 1397
HincII	GTYRAC	2 cut(s)	1211, 1834
HindII	GTYRAC	2 cut(s)	1211, 1834
HindIII	AAGCTT	3 cut(s)	962, 1504, 2306
HpaII	CCGG	2 cut(s)	271, 350
HphI	GGTGA	3 cut(s)	154, 604, 1651
Hpy166II	GTNNAC	7 cut(s)	94, 116, 1172, 1211, 1244, 1265, 1834
Hpy8I	GTNNAC	7 cut(s)	94, 116, 1172, 1211, 1244, 1265, 1834
Hpy99I	CGWCG	2 cut(s)	193, 590
HpyAV	CCTTC	8 cut(s)	538, 746, 897, 1042, 1084, 1745, 2032, 2439
HpyCH4III	ACNGT	8 cut(s)	113, 771, 806, 990, 1663, 1901, 1998, 2326
HpyCH4IV	ACGT	1 cut(s)	1830
HpySE526I	ACGT	1 cut(s)	1830
HspAI	GCGC	4 cut(s)	224, 287, 330, 1397
Kzo9I	GATC	9 cut(s)	249, 1016, 1428, 1614, 1647, 1730, 1925, 2043, 2296
LmnI	GCTCC	7 cut(s)	353, 491, 738, 1203, 1870, 1923, 2006
Lsp1109I	GCAGC	6 cut(s)	71, 667, 859, 1300, 1565, 1703
LweI	GCATC	5 cut(s)	509, 557, 643, 1161, 2361
MaeI	CTAG	3 cut(s)	627, 1620, 2187
MaeII	ACGT	1 cut(s)	1830
MaeIII	GTNAC	3 cut(s)	296, 1992, 2320
MalI	GATC	9 cut(s)	251, 1018, 1430, 1616, 1649, 1732, 1927, 2045, 2298
MboI	GATC	9 cut(s)	249, 1016, 1428, 1614, 1647, 1730, 1925, 2043, 2296
MboII	GAAGA	7 cut(s)	1139, 1374, 1472, 1868, 2030, 2222, 2231
MfeI	CAATTG	2 cut(s)	1186, 1338
MflI	RGATCY	4 cut(s)	1016, 1428, 1730, 1925
MhlI	GDGCHC	4 cut(s)	981, 1148, 1567, 2003
MlsI	TGGCCA	1 cut(s)	1533
MluNI	TGGCCA	1 cut(s)	1533
MlyI	GAGTC	2 cut(s)	1326, 2162
MmeI	TCCRAC	2 cut(s)	1989, 2286
Mox20I	TGGCCA	1 cut(s)	1533
MroXI	GAANNNNTTC	1 cut(s)	635
MscI	TGGCCA	1 cut(s)	1533
MseI	TTAA	5 cut(s)	909, 1257, 1305, 2288, 2381
MslI	CAYNNNNRTG	2 cut(s)	17, 1958
Msp20I	TGGCCA	1 cut(s)	1533
MspA1I	CMGCKG	3 cut(s)	1291, 1556, 1867
MspI	CCGG	2 cut(s)	271, 350
MspR9I	CCNGG	3 cut(s)	16, 351, 2372
MunI	CAATTG	2 cut(s)	1186, 1338
Mva1269I	GAATGC	3 cut(s)	628, 1136, 2225
MvaI	CCWGG	2 cut(s)	16, 2372
NciI	CCSGG	1 cut(s)	351
NdeII	GATC	9 cut(s)	249, 1016, 1428, 1614, 1647, 1730, 1925, 2043, 2296
NlaIV	GGNNCC	7 cut(s)	44, 493, 1040, 1732, 1791, 1872, 1903
NmeAIII	GCCGAG	3 cut(s)	476, 643, 1557
NspI	RCATGY	2 cut(s)	224, 2440
NspV	TTCGAA	2 cut(s)	67, 739
PaeI	GCATGC	1 cut(s)	224
PaeR7I	CTCGAG	1 cut(s)	1392
PagI	TCATGA	1 cut(s)	1057
PceI	AGGCCT	2 cut(s)	1031, 1278
PcsI	WCGNNNNNNNCGW	1 cut(s)	933
PctI	GAATGC	3 cut(s)	628, 1136, 2225
PdmI	GAANNNNTTC	1 cut(s)	635
PfeI	GAWTC	9 cut(s)	49, 463, 1623, 1669, 1945, 1976, 2012, 2198, 2231
PflMI	CCANNNNNTGG	1 cut(s)	2107
PkrI	GCNGC	7 cut(s)	61, 559, 682, 874, 1290, 1555, 1693
Ple19I	CGATCG	1 cut(s)	1617
PleI	GAGTC	2 cut(s)	1326, 2161
PpsI	GAGTC	2 cut(s)	1326, 2161
PshBI	ATTAAT	1 cut(s)	2288
Psp6I	CCWGG	2 cut(s)	14, 2370
PspFI	CCCAGC	3 cut(s)	193, 1291, 2365
PspGI	CCWGG	2 cut(s)	14, 2370
PspN4I	GGNNCC	7 cut(s)	44, 493, 1040, 1732, 1791, 1872, 1903
PspPI	GGNCC	7 cut(s)	27, 113, 281, 1038, 1595, 1790, 2368
PspXI	VCTCGAGB	1 cut(s)	1392
PstNI	CAGNNNCTG	3 cut(s)	437, 1556, 1634
PsuI	RGATCY	4 cut(s)	1016, 1428, 1730, 1925
PvuI	CGATCG	1 cut(s)	1617
PvuII	CAGCTG	3 cut(s)	1291, 1556, 1867
RgaI	GCGATCGC	1 cut(s)	1617
RsaI	GTAC	3 cut(s)	450, 481, 647
RsaNI	GTAC	3 cut(s)	449, 480, 646
RseI	CAYNNNNRTG	2 cut(s)	17, 1958
SalI	GTCGAC	1 cut(s)	1209
SaqAI	TTAA	5 cut(s)	909, 1257, 1305, 2288, 2381
SatI	GCNGC	7 cut(s)	60, 558, 681, 873, 1289, 1554, 1692
Sau3AI	GATC	9 cut(s)	249, 1016, 1428, 1614, 1647, 1730, 1925, 2043, 2296
Sau96I	GGNCC	7 cut(s)	27, 113, 281, 1038, 1595, 1790, 2368
ScaI	AGTACT	1 cut(s)	481
SchI	GAGTC	2 cut(s)	1326, 2162
ScrFI	CCNGG	3 cut(s)	16, 351, 2372
SduI	GDGCHC	4 cut(s)	981, 1148, 1567, 2003
SfaAI	GCGATCGC	1 cut(s)	1617
SfaNI	GCATC	5 cut(s)	509, 557, 643, 1161, 2361
SfcI	CTRYAG	1 cut(s)	1557
Sfr274I	CTCGAG	1 cut(s)	1392
SfuI	TTCGAA	2 cut(s)	67, 739
SgfI	GCGATCGC	1 cut(s)	1617
SinI	GGWCC	4 cut(s)	27, 113, 1038, 1790
SlaI	CTCGAG	1 cut(s)	1392
SmiMI	CAYNNNNRTG	2 cut(s)	17, 1958
SmlI	CTYRAG	7 cut(s)	666, 853, 965, 974, 1162, 1392, 1465
SmoI	CTYRAG	7 cut(s)	666, 853, 965, 974, 1162, 1392, 1465
SphI	GCATGC	1 cut(s)	224
SseBI	AGGCCT	2 cut(s)	1031, 1278
SsiI	CCGC	4 cut(s)	376, 557, 923, 1167
SspMI	CTAG	3 cut(s)	627, 1620, 2187
StuI	AGGCCT	2 cut(s)	1031, 1278
StyD4I	CCNGG	3 cut(s)	14, 349, 2370
StyI	CCWWGG	3 cut(s)	711, 1487, 2134
TaaI	ACNGT	8 cut(s)	113, 771, 806, 990, 1663, 1901, 1998, 2326
TaiI	ACGT	1 cut(s)	1833
TatI	WGTACW	2 cut(s)	479, 645
TauI	GCSGC	1 cut(s)	560
TfiI	GAWTC	9 cut(s)	49, 463, 1623, 1669, 1945, 1976, 2012, 2198, 2231
Tru1I	TTAA	5 cut(s)	909, 1257, 1305, 2288, 2381
Tru9I	TTAA	5 cut(s)	909, 1257, 1305, 2288, 2381
TscAI	CASTG	7 cut(s)	210, 324, 351, 421, 1290, 1791, 2003
TseI	GCWGC	6 cut(s)	59, 680, 872, 1288, 1553, 1691
TspRI	CASTG	7 cut(s)	210, 324, 351, 421, 1290, 1791, 2003
Van91I	CCANNNNNTGG	1 cut(s)	2107
VpaK11BI	GGWCC	4 cut(s)	27, 113, 1038, 1790
VspI	ATTAAT	1 cut(s)	2288
XapI	RAATTY	3 cut(s)	1098, 2083, 2245
XceI	RCATGY	2 cut(s)	224, 2440
XhoI	CTCGAG	1 cut(s)	1392
XmiI	GTMKAC	3 cut(s)	93, 1210, 1243
XmnI	GAANNNNTTC	1 cut(s)	635
XspI	CTAG	3 cut(s)	627, 1620, 2187
ZrmI	AGTACT	1 cut(s)	481

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.