MD12G1140100.v1.1

Fusaric acid resistance protein-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr12
OG0003101
Physical Location & Seq
Reverse (-)
21793089 .. 21800357
7269 bp
Loading structure...
UTR
Exon/CDS
Intron
MD12G1140100.v1.1.491

Sequence Viewer

Length: 3855 bp
ATGACCACAATCCTAATAGCCCCGGACGCAACGCTTGGGGACGTCATGAGAAGCTGTTGGCATGTGATCTTTGCCACGGCACAGGTACTGTTGTCTTCTGTGCTGACCCTTTGGCTGGTGGAGCCTAAAAACTTCACGGTGGAGGTAGCGGCGGTGGCAGTTGCTCTGAGTGCTTTTGTGGTGGTTTTGCCGGAGTCCACGCACTTGTTGTCGAAGAGAATTGCGTTTGGGCAGTTTGTGATTGTTTATGTTGGCACTGTGATTCATGGAGCACAAACTGGGGTTGTCATGCATCCACTTGGTGTTGCATCAAGCACTGCCCTTGGAGCTTTCGCTTCTGTTGTTGCTATGTTGTTTCCTTATCCTCGGCTATCTTACTATGTGGTCAGTAAATCATGGCGCATGTATGCCGGAAATGCTTCTCAGAGGTTAGCTTGCTTCGTTGAGGCCATCTCTTCCCGGGACAAGAGCGGAGCGCTCGAATTCCTCTCTCGGGGACAATCTCTCTCCAAAGAAGCAGCTAAGCTTCTTCAAAGCATCAGTAATAATCTGGAAACCATGGTGTGGGAGAGACCACATATCAAATTTCTGAAACCAAATTATCTGGACTCAGGTGAAAGGTTGCAAGAAATTGAGGTTCCACTGAGGGGGATCGAAATAGCTTTATCTAGTTGCTCTTCACATCCTGTAAACCTAATTGATGAAGACCTAAGACGCAATTTACAAAGTTCAGAAGTAGAGGTTAGCCTAAGGCTTCTGCAAGCTAAGTACTCTTTGCCTTCCGATGCAACATTGGCTCCGAAGTCATACAGAGAAATCTTCGACAAGCCACTTTGGGCCGGAAAACCCACCACCAATAACCGTGAGAATCTTCCGGCTTTTTTCCTCTTGTACTGCATGGAACTCCTCCTAGAAAACCAACCCATTGCCTGGAATCCTCTAAACACTAGGAAACCTAACCAACAACCCAGTGATTCGCAAAATCAACGGCGGTGGAACTTCAAAACTGTTTGGAGAAACATAATCCCAAGTACCAGGAGTATAATTTTTGCTCTCAAGTGCTCACTTGCATTAGGCCTTGCTGTGTTATTCGGCTTGTTGTATAACAAGGAAAACGGGTACTGGTCAGGCCTCGCCATTGCCTTCAGCTTTGTAACAGGACGGCAAGCAACATTCACAGTTACAAATGCTCGAGTTCAAGGAATGGCACTGGGATCGATCTATGGAATCATATGGTTATTTATTTTCCAAAGACTTGAGAAATTCAAGCTCTTACCACTCATACCTTGGATCTTTTTCTGCCATTTTCTTAGGCATAGCAGGATGTATGGCCAAGCTGGTGGGATCTCGGCAGCCATTGCAGCATTACTTATCTTGGGTAGGGAAAACCATGGTCCTCCAAGTGAATTTGCATTTGCAAGAATGATAGAGGCATCCATTGGACTACTTTGTTTCCTTTTAGTAGAGATTGTCTTTTATCCGATGAGAGCAGCGACCCTAGCGAAAAATAAGCTCTCGCAAAGCATGGAGGCGCTTCGAGATTGTATAGGAGGAGACGTAAGTAGTGATATGTCTAAGAATATAGTTAAAGATATGCAAGATCGTGGAGAAGGTAACGAAAAAAAGAGCAAGGCAATGTGGGATGATGCAAGTGTTGAGATATTTATAAGTGTTTGTATAGCTGAGACATTAGCCGGAAATCAAACAGGTGGTCATCTTAATAGAATTGGATGGAAAAATGTTATTAAAAAATTTAATGACCTTACTCAAAGGTCATATGTTCATAAACAACTCAAGAACAAATGGACTGCACTTAAGAAAGAATGGCAATTGTGGGCATCATTAGTTGGAAAAGAGACTGGCTTAGGATGGGATCCTGTGAAGCAAACTATCATTGCAAGTGATGAGTGGTGGGAGAAAAAAGTGAAGGAGAATTCTGAAGTTGCAAAATATCGAAATAGTGGATTGAAAAATGTTGACCAACTGGACATTTTGTTTAAAGAAGGAGCTGTTACAGGTGTAGGTGCATGGGCACCCTCACAAGGGTTGATGAGTAATAATGTCCAAAAAGCTCCAACATGCGAACAAAATGACGGTCAAGATATGAGTTCTAGCAGCAGTGATAGTAGCAATACAAACAACTCTACTGATGGAAGTGATGATGAATTTTATCAACTGGTTTCTGCAAGCTGTGTCACGGCTGTCTCTGAATGGTTAAAGTATGTTGACAAAGAACCTTGTAGGACTTCTTCGTATACGGGATATAGATGGGTAATGGAAGTTTTAAATGGTCATCCTATAAGATGCCATGAACAATTTAGGATGGAACAACATGTTTTTAAGGACTTGTTAGAGACTTTGAAGCGTGATTATGGCTTAAAAAGAATTGGAGATATAACCCTAGAAGAAGCATTAGCAATGTTTCTAAACACATTAGGTCATGGGCACACTAATAGGATGGTCCAAGAAAGGTTTCAACGCTCTGGAGAGACTGTTTCTAGGTGGTTTGGGATACTATTAGATGTTGTTTCTCGCATGGCTACTGATATTATATGTCCACATGATCGGCAATTCAAGAGAGTATCAAATAAAATTAAGGATGACAATCGGTATTGGCCATATTTTAAAGGTTGCATAGGGGCAATTGACGGAACACATGTCCCAGTTGTGGTACCTAGAGAAATGCAAGTGCCATATATAGGTAGAAAAGGAAATACAACTCAAAATGTTATGGCTGTATGTGATTTTAATATGTGTTTCACATTTGTCTGGGCTGGATGTGAGGGTGCTGCTCATGATTCACGTATATTCATGGAGGCCTTGAGAAGACCAGAATTGAAATTTCCTTATCCCCCTTCTGGCAAATATTATTTAGTTGATGCTGGATATCCACATATGAATGGATATATTGGACCTTATAGGGGTGAACGATATCATCTTCCTGAATTTCGACGTGGAAGTCATCCAAGAGGGAAAAAAGAGATATTTAATCAGAGACATTCATCCTTAAGATGCACAATAGAAAGAACTTTTGGTGTTTGGAAAAATAGATGGACAATGATTCGTCTCATGCGTAACTTCCCTTTTGAAAAACAGGTACAAATTGTTGTTGCTTCAATGGCCTTACACAATTTTATTAGGAAGCATTCCATGACAGATCAAGAGTTCCAACCTTATGATGATGATGATGAGCTACTTCCACCTGGACATGAGGAAGATCATAGAGATGAAGAAATTGTTGATGAAAATTTTACTCATAGGCGAGAGATGGATAGGAAGCTCAAGTCCCATGTCAAAGAACTAGAAACATTCATCCAAGAAGCTGAAACAGAGCCTAATTGCTGGTTCTTGCCCTTCCAGGGTGCCAGTTACAATCGGGTCCTAGGATCTTTATCAAAAATCGCTGATCTTTTACTATTTGTGGCCTATAAGACGGAGTTTCTCGCACAAGTAGAGCAGAACTTTGGAGGTGTTTGGGAGGAACTAAGGCAACAAATGAATGCTGATCTAGAGCTGCTAAAGGGTTGCCTTGAGGAGGCGTCTTCGATTAAGTCCCTGCCGGTATCCGAGACAATGTCGGAAAGTGATTACCATGACAGTGATTTGGGCAAACCACCGAGGGGATTTGCGACTTTGGGTTGTACTGATGATGTTGAGACTATTGTGAGTAGCTTTCTTCAACATTTGCAGGAAGTGGCTGACAAGGTACATACTAGTGACAGTGAAGAGAAACATAAGAGCCAAATGGTTCTTTGTTTGACTAGCCTGGGATTCTGCATTCCAAGCGTGATGAGAGAAACAATGGAGATGAAGAAAGAAGTGAGAAAACTAGTCAAATTGAATGCGCCTACATAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
FUSC FUSC_2

Protein Analysis

1285

Amino Acids

145.59

Weight (kDa)

7.73

Isoelectric Point (pI)

40.79

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
FUSC PF04632 348 - 529 2.6e-11 Fusaric acid resistance protein family
FUSC_2 PF13515 360 - 488 1.7e-13 Fusaric acid resistance protein-like
Myb_DNA-bind_3 PF12776 547 - 640 8.8e-20 Myb/SANT-like DNA-binding domain
DUF8040 PF26138 749 - 844 1.4e-34 Domain of unknown function (DUF8040)
DDE_Tnp_1 PF01609 876 - 1036 5.9e-08 Transposase DDE domain
DDE_Tnp_4 PF13359 882 - 1044 3.2e-22 DDE superfamily endonuclease
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 1667
AasI GACNNNNNNGTC 1 cut(s) 2958
AatII GACGTC 1 cut(s) 45
Acc65I GGTACC 1 cut(s) 2671
AccB1I GGYRCC 3 cut(s) 2032, 2671, 3362
AccB7I CCANNNNNTGG 2 cut(s) 564, 930
AccBSI CCGCTC 1 cut(s) 471
AccI GTMKAC 1 cut(s) 2255
AciI CCGC 4 cut(s) 149, 152, 471, 991
AclWI GGATC 7 cut(s) 659, 1222, 1298, 1352, 1868, 1881, 3394
AcoI YGGCCR 2 cut(s) 1330, 2615
AcuI CTGAAG 2 cut(s) 1129, 1959
AcyI GRCGYC 2 cut(s) 42, 3539
AdeI CACNNNGTG 1 cut(s) 302
AfaI GTAC 9 cut(s) 87, 770, 893, 1033, 1121, 2673, 3099, 3643, 3708
AfeI AGCGCT 1 cut(s) 477
AflII CTTAAG 2 cut(s) 1814, 3007
AflIII ACRYGT 2 cut(s) 2332, 2656
AhlI ACTAGT 2 cut(s) 3713, 3829
AjiI CACGTC 1 cut(s) 2954
AjnI CCWGG 5 cut(s) 929, 1034, 3202, 3357, 3765
AjuI GAANNNNNNNTTGG 4 cut(s) 2457, 2489, 3015, 3047
Alw21I GWGCWC 2 cut(s) 274, 1064
AlwI GGATC 7 cut(s) 659, 1222, 1298, 1352, 1868, 1881, 3394
AlwNI CAGNNNCTG 1 cut(s) 88
Ama87I CYCGRG 3 cut(s) 459, 492, 1191
Aor51HI AGCGCT 1 cut(s) 477
AoxI GGCC 9 cut(s) 447, 837, 1075, 1129, 1330, 2615, 2817, 3120, 3423
ApeKI GCWGC 7 cut(s) 518, 1352, 1361, 1490, 2115, 2789, 3514
ArsI GACNNNNNNTTYG 4 cut(s) 1696, 1728, 2080, 2112
Asp700I GAANNNNTTC 2 cut(s) 418, 2472
Asp718I GGTACC 1 cut(s) 2671
AspA2I CCTAGG 1 cut(s) 3382
AspLEI GCGC 4 cut(s) 402, 478, 1534, 3847
AspS9I GGNCC 5 cut(s) 837, 1394, 2461, 2912, 3379
AsuC2I CCSGG 3 cut(s) 23, 460, 461
AsuHPI GGTGA 2 cut(s) 626, 2936
AvaI CYCGRG 3 cut(s) 459, 492, 1191
AvaII GGWCC 4 cut(s) 1394, 2461, 2912, 3379
AvrII CCTAGG 1 cut(s) 3382
AxyI CCTNAGG 1 cut(s) 749
BaeGI GKGCMC 2 cut(s) 2035, 2448
BaeI ACNNNNGTAYC 2 cut(s) 77, 110
BalI TGGCCA 2 cut(s) 1332, 2617
BamHI GGATCC 1 cut(s) 1873
BanI GGYRCC 3 cut(s) 2032, 2671, 3362
BarI GAAGNNNNNNTAC 2 cut(s) 2233, 2265
BbsI GAAGAC 4 cut(s) 87, 711, 2833, 3534
Bbv12I GWGCWC 2 cut(s) 274, 1064
BbvI GCAGC 7 cut(s) 530, 1364, 1373, 1502, 2127, 2776, 3501
BccI CCATC 9 cut(s) 458, 1725, 1863, 2144, 2262, 2317, 2452, 3045, 3262
BceAI ACGGC 4 cut(s) 93, 1004, 1178, 2214
BcgI CGANNNNNNTGC 2 cut(s) 1197, 1231
BciT130I CCWGG 5 cut(s) 931, 1036, 3204, 3359, 3767
BciVI GTATCC 2 cut(s) 2505, 3574
BcnI CCSGG 3 cut(s) 23, 460, 461
BcuI ACTAGT 2 cut(s) 3713, 3829
BfoI RGCGCY 2 cut(s) 479, 1535
BfrI CTTAAG 2 cut(s) 1814, 3007
BfuI GTATCC 2 cut(s) 2505, 3574
BisI GCNGC 8 cut(s) 150, 519, 1353, 1362, 1491, 2116, 2790, 3515
BlnI CCTAGG 1 cut(s) 3382
BlpI GCTNAGC 1 cut(s) 522
BlsI GCNGC 8 cut(s) 151, 520, 1354, 1363, 1492, 2117, 2791, 3516
BmcAI AGTACT 1 cut(s) 770
Bme1390I CCNGG 8 cut(s) 23, 460, 461, 931, 1036, 3204, 3359, 3767
Bme18I GGWCC 4 cut(s) 1394, 2461, 2912, 3379
BmeT110I CYCGRG 3 cut(s) 459, 492, 1191
BmgBI CACGTC 1 cut(s) 2954
BmgT120I GGNCC 5 cut(s) 837, 1394, 2461, 2912, 3379
BmiI GGNNCC 8 cut(s) 123, 639, 798, 1875, 2034, 2673, 3364, 3380
BmrFI CCNGG 8 cut(s) 23, 460, 461, 931, 1036, 3204, 3359, 3767
BmrI ACTGGG 4 cut(s) 288, 963, 1220, 2657
BmuI ACTGGG 4 cut(s) 288, 963, 1220, 2657
BpiI GAAGAC 4 cut(s) 87, 711, 2833, 3534
BplI GAGNNNNNCTC 2 cut(s) 437, 469
BpmI CTGGAG 1 cut(s) 2505
Bpu10I CCTNAGC 1 cut(s) 1864
Bpu1102I GCTNAGC 1 cut(s) 522
BpuEI CTTGAG 6 cut(s) 1040, 1277, 1778, 2842, 3266, 3551
BpuMI CCSGG 3 cut(s) 23, 460, 461
Bsa29I ATCGAT 1 cut(s) 1217
BsaAI YACGTR 1 cut(s) 2804
BsaBI GATNNNNATC 2 cut(s) 2604, 3391
BsaHI GRCGYC 2 cut(s) 42, 3539
BsaI GGTCTC 1 cut(s) 565
BsaXI ACNNNNNCTCC 6 cut(s) 781, 811, 1542, 1572, 2478, 2508
Bse118I RCCGGY 1 cut(s) 3559
Bse1I ACTGG 9 cut(s) 283, 969, 1127, 1215, 1864, 1989, 2181, 2663, 3366
Bse21I CCTNAGG 1 cut(s) 749
Bse3DI GCAATG 6 cut(s) 924, 1137, 1356, 1641, 1893, 2424
Bse8I GATNNNNATC 2 cut(s) 2604, 3391
BseBI CCWGG 5 cut(s) 931, 1036, 3204, 3359, 3767
BseCI ATCGAT 1 cut(s) 1217
BseJI GATNNNNATC 2 cut(s) 2604, 3391
BseMI GCAATG 6 cut(s) 924, 1137, 1356, 1641, 1893, 2424
BseMII CTCAG 5 cut(s) 158, 437, 624, 635, 1674
BseNI ACTGG 9 cut(s) 283, 969, 1127, 1215, 1864, 1989, 2181, 2663, 3366
BseRI GAGGAG 3 cut(s) 896, 1566, 3548
BseSI GKGCMC 2 cut(s) 2035, 2448
BseXI GCAGC 7 cut(s) 530, 1364, 1373, 1502, 2127, 2776, 3501
BsgI GTGCAG 1 cut(s) 1794
BshFI GGCC 9 cut(s) 449, 839, 1077, 1131, 1332, 2617, 2819, 3122, 3425
BshNI GGYRCC 3 cut(s) 2032, 2671, 3362
BshVI ATCGAT 1 cut(s) 1217
BsiHKAI GWGCWC 2 cut(s) 274, 1064
BsiHKCI CYCGRG 3 cut(s) 459, 492, 1191
BsiSI CCGG 8 cut(s) 23, 191, 411, 460, 840, 875, 1695, 3560
BslFI GGGAC 6 cut(s) 53, 476, 510, 2645, 3271, 3538
BsmBI CGTCTC 2 cut(s) 1548, 3071
BsmFI GGGAC 6 cut(s) 53, 476, 510, 2645, 3271, 3538
BsmI GAATGC 4 cut(s) 3145, 3505, 3777, 3847
BsnI GGCC 9 cut(s) 449, 839, 1077, 1131, 1332, 2617, 2819, 3122, 3425
Bso31I GGTCTC 1 cut(s) 565
BsoBI CYCGRG 3 cut(s) 459, 492, 1191
Bsp1286I GDGCHC 4 cut(s) 274, 1064, 2035, 2448
Bsp1720I GCTNAGC 1 cut(s) 522
Bsp19I CCATGG 2 cut(s) 558, 1390
BspACI CCGC 4 cut(s) 149, 152, 471, 991
BspANI GGCC 9 cut(s) 449, 839, 1077, 1131, 1332, 2617, 2819, 3122, 3425
BspCNI CTCAG 5 cut(s) 159, 436, 623, 636, 1675
BspDI ATCGAT 1 cut(s) 1217
BspHI TCATGA 2 cut(s) 45, 2794
BspLI GGNNCC 8 cut(s) 123, 639, 798, 1875, 2034, 2673, 3364, 3380
BspPI GGATC 7 cut(s) 659, 1222, 1298, 1352, 1868, 1881, 3394
BspQI GCTCTTC 1 cut(s) 682
BspT107I GGYRCC 3 cut(s) 2032, 2671, 3362
BspTI CTTAAG 2 cut(s) 1814, 3007
BspTNI GGTCTC 1 cut(s) 565
BsrBI CCGCTC 1 cut(s) 471
BsrDI GCAATG 6 cut(s) 924, 1137, 1356, 1641, 1893, 2424
BsrFI RCCGGY 1 cut(s) 3559
BsrI ACTGG 9 cut(s) 283, 969, 1127, 1215, 1864, 1989, 2181, 2663, 3366
BssAI RCCGGY 1 cut(s) 3559
BssNAI GTATAC 1 cut(s) 2256
BssNI GRCGYC 2 cut(s) 42, 3539
BssT1I CCWWGG 5 cut(s) 322, 558, 1286, 1390, 3382
Bst1107I GTATAC 1 cut(s) 2256
Bst2UI CCWGG 5 cut(s) 931, 1036, 3204, 3359, 3767
Bst6I CTCTTC 4 cut(s) 209, 460, 682, 3720
BstACI GRCGYC 2 cut(s) 42, 3539
BstAFI CTTAAG 2 cut(s) 1814, 3007
BstAPI GCANNNNNTGC 1 cut(s) 1358
BstBAI YACGTR 1 cut(s) 2804
BstC8I GCNNGC 4 cut(s) 436, 762, 1167, 2188
BstDSI CCRYGG 3 cut(s) 75, 558, 1390
BstENI CCTNNNNNAGG 1 cut(s) 3533
BstH2I RGCGCY 2 cut(s) 479, 1535
BstHHI GCGC 4 cut(s) 402, 478, 1534, 3847
BstNI CCWGG 5 cut(s) 931, 1036, 3204, 3359, 3767
BstNSI RCATGY 5 cut(s) 65, 406, 2082, 2336, 2660
BstSCI CCNGG 8 cut(s) 21, 458, 459, 929, 1034, 3202, 3357, 3765
BstSLI GKGCMC 2 cut(s) 2035, 2448
BstV1I GCAGC 7 cut(s) 530, 1364, 1373, 1502, 2127, 2776, 3501
BstV2I GAAGAC 4 cut(s) 87, 711, 2833, 3534
BstX2I RGATCY 4 cut(s) 1290, 1344, 1873, 3386
BstXI CCANNNNNNTGG 1 cut(s) 1340
BstYI RGATCY 4 cut(s) 1290, 1344, 1873, 3386
BstZ17I GTATAC 1 cut(s) 2256
Bsu15I ATCGAT 1 cut(s) 1217
Bsu36I CCTNAGG 1 cut(s) 749
BsuI GTATCC 2 cut(s) 2505, 3574
BsuRI GGCC 9 cut(s) 449, 839, 1077, 1131, 1332, 2617, 2819, 3122, 3425
BsuTUI ATCGAT 1 cut(s) 1217
BtgI CCRYGG 3 cut(s) 75, 558, 1390
BtrI CACGTC 1 cut(s) 2954
BtsI GCAGTG 2 cut(s) 315, 2125
BtsIMutI CAGTG 8 cut(s) 255, 315, 641, 976, 1208, 2125, 3604, 3727
Cac8I GCNNGC 4 cut(s) 436, 762, 1167, 2188
CaiI CAGNNNCTG 1 cut(s) 88
CciI TCATGA 2 cut(s) 45, 2794
CfoI GCGC 4 cut(s) 402, 478, 1534, 3847
Cfr10I RCCGGY 1 cut(s) 3559
Cfr13I GGNCC 5 cut(s) 837, 1394, 2461, 2912, 3379
Cfr9I CCCGGG 1 cut(s) 459
ClaI ATCGAT 1 cut(s) 1217
CseI GACGC 3 cut(s) 35, 723, 3528
Csp6I GTAC 9 cut(s) 86, 769, 892, 1032, 1120, 2672, 3098, 3642, 3707
CspCI CAANNNNNGTGG 2 cut(s) 974, 1009
CviQI GTAC 9 cut(s) 86, 769, 892, 1032, 1120, 2672, 3098, 3642, 3707
DraI TTTAAA 3 cut(s) 1999, 2286, 2626
DraIII CACNNNGTG 1 cut(s) 302
DrdI GACNNNNNNGTC 1 cut(s) 2958
DseDI GACNNNNNNGTC 1 cut(s) 2958
EaeI YGGCCR 2 cut(s) 1330, 2615
Eam1104I CTCTTC 4 cut(s) 209, 460, 682, 3720
EarI CTCTTC 4 cut(s) 209, 460, 682, 3720
Eco130I CCWWGG 5 cut(s) 322, 558, 1286, 1390, 3382
Eco147I AGGCCT 3 cut(s) 1077, 1131, 2819
Eco31I GGTCTC 1 cut(s) 565
Eco32I GATATC 2 cut(s) 2888, 2933
Eco47I GGWCC 4 cut(s) 1394, 2461, 2912, 3379
Eco47III AGCGCT 1 cut(s) 477
Eco57I CTGAAG 2 cut(s) 1129, 1959
Eco81I CCTNAGG 1 cut(s) 749
Eco88I CYCGRG 3 cut(s) 459, 492, 1191
EcoNI CCTNNNNNAGG 1 cut(s) 3533
EcoO109I RGGNCCY 1 cut(s) 3379
EcoRI GAATTC 2 cut(s) 482, 1933
EcoRII CCWGG 5 cut(s) 929, 1034, 3202, 3357, 3765
EcoRV GATATC 2 cut(s) 2888, 2933
EcoT14I CCWWGG 5 cut(s) 322, 558, 1286, 1390, 3382
EcoT22I ATGCAT 1 cut(s) 294
ErhI CCWWGG 5 cut(s) 322, 558, 1286, 1390, 3382
Esp3I CGTCTC 2 cut(s) 1548, 3071
FaqI GGGAC 6 cut(s) 53, 476, 510, 2645, 3271, 3538
FauNDI CATATG 3 cut(s) 1232, 1777, 2895
FblI GTMKAC 1 cut(s) 2255
Fnu4HI GCNGC 8 cut(s) 150, 519, 1353, 1362, 1491, 2116, 2790, 3515
Fsp4HI GCNGC 8 cut(s) 150, 519, 1353, 1362, 1491, 2116, 2790, 3515
GlaI GCGC 4 cut(s) 401, 477, 1533, 3846
GluI GCNGC 8 cut(s) 150, 519, 1353, 1362, 1491, 2116, 2790, 3515
GsuI CTGGAG 1 cut(s) 2505
HaeII RGCGCY 2 cut(s) 479, 1535
HaeIII GGCC 9 cut(s) 449, 839, 1077, 1131, 1332, 2617, 2819, 3122, 3425
HapII CCGG 8 cut(s) 23, 191, 411, 460, 840, 875, 1695, 3560
HgaI GACGC 3 cut(s) 35, 723, 3528
HhaI GCGC 4 cut(s) 402, 478, 1534, 3847
Hin1I GRCGYC 2 cut(s) 42, 3539
Hin6I GCGC 4 cut(s) 400, 476, 1532, 3845
HinP1I GCGC 4 cut(s) 400, 476, 1532, 3845
HincII GTYRAC 2 cut(s) 1978, 2227
HindII GTYRAC 2 cut(s) 1978, 2227
HindIII AAGCTT 1 cut(s) 524
HpaII CCGG 8 cut(s) 23, 191, 411, 460, 840, 875, 1695, 3560
HphI GGTGA 2 cut(s) 626, 2936
Hpy166II GTNNAC 7 cut(s) 198, 691, 1978, 2227, 2256, 2558, 2927
Hpy8I GTNNAC 7 cut(s) 198, 691, 1978, 2227, 2256, 2558, 2927
Hpy99I CGWCG 1 cut(s) 2955
HpyAV CCTTC 7 cut(s) 789, 1153, 1604, 1921, 1997, 2865, 3364
HpyCH4IV ACGT 4 cut(s) 42, 1557, 2803, 2953
HpySE526I ACGT 4 cut(s) 42, 1557, 2803, 2953
Hsp92I GRCGYC 2 cut(s) 42, 3539
HspAI GCGC 4 cut(s) 400, 476, 1532, 3845
KpnI GGTACC 1 cut(s) 2675
LguI GCTCTTC 1 cut(s) 682
LmnI GCTCC 7 cut(s) 121, 269, 326, 473, 802, 2006, 2077
Lsp1109I GCAGC 7 cut(s) 530, 1364, 1373, 1502, 2127, 2776, 3501
MaeII ACGT 4 cut(s) 42, 1557, 2803, 2953
MaeIII GTNAC 8 cut(s) 1153, 1180, 1613, 2011, 2194, 3074, 3368, 3716
MbiI CCGCTC 1 cut(s) 471
MfeI CAATTG 2 cut(s) 1829, 2643
MflI RGATCY 4 cut(s) 1290, 1344, 1873, 3386
MhlI GDGCHC 4 cut(s) 274, 1064, 2035, 2448
MlsI TGGCCA 2 cut(s) 1332, 2617
MluNI TGGCCA 2 cut(s) 1332, 2617
MlyI GAGTC 2 cut(s) 203, 602
MmeI TCCRAC 4 cut(s) 1828, 2099, 3193, 3558
Mox20I TGGCCA 2 cut(s) 1332, 2617
Mph1103I ATGCAT 1 cut(s) 294
MroXI GAANNNNTTC 2 cut(s) 418, 2472
MscI TGGCCA 2 cut(s) 1332, 2617
MslI CAYNNNNRTG 4 cut(s) 2898, 3225, 3597, 3714
Msp20I TGGCCA 2 cut(s) 1332, 2617
MspCI CTTAAG 2 cut(s) 1814, 3007
MspI CCGG 8 cut(s) 23, 191, 411, 460, 840, 875, 1695, 3560
MspR9I CCNGG 8 cut(s) 23, 460, 461, 931, 1036, 3204, 3359, 3767
MunI CAATTG 2 cut(s) 1829, 2643
Mva1269I GAATGC 4 cut(s) 3145, 3505, 3777, 3847
MvaI CCWGG 5 cut(s) 931, 1036, 3204, 3359, 3767
NciI CCSGG 3 cut(s) 23, 460, 461
NcoI CCATGG 2 cut(s) 558, 1390
NdeI CATATG 3 cut(s) 1232, 1777, 2895
NlaIV GGNNCC 8 cut(s) 123, 639, 798, 1875, 2034, 2673, 3364, 3380
NmeAIII GCCGAG 2 cut(s) 346, 1328
NmuCI GTSAC 2 cut(s) 2194, 3716
NsiI ATGCAT 1 cut(s) 294
NspI RCATGY 5 cut(s) 65, 406, 2082, 2336, 2660
PaeR7I CTCGAG 1 cut(s) 1191
PagI TCATGA 2 cut(s) 45, 2794
PceI AGGCCT 3 cut(s) 1077, 1131, 2819
PciI ACATGT 2 cut(s) 2332, 2656
PciSI GCTCTTC 1 cut(s) 682
PcsI WCGNNNNNNNCGW 1 cut(s) 3070
PctI GAATGC 4 cut(s) 3145, 3505, 3777, 3847
PdmI GAANNNNTTC 2 cut(s) 418, 2472
PfeI GAWTC 8 cut(s) 262, 868, 934, 974, 1227, 2798, 3061, 3771
PflFI GACNNNGTC 1 cut(s) 3574
PflMI CCANNNNNTGG 2 cut(s) 564, 930
PkrI GCNGC 8 cut(s) 151, 520, 1354, 1363, 1492, 2117, 2791, 3516
PleI GAGTC 2 cut(s) 202, 602
PpsI GAGTC 2 cut(s) 202, 602
Ppu21I YACGTR 1 cut(s) 2804
PpuMI RGGWCCY 1 cut(s) 3379
PscI ACATGT 2 cut(s) 2332, 2656
PsiI TTATAA 1 cut(s) 1667
Psp5II RGGWCCY 1 cut(s) 3379
Psp6I CCWGG 5 cut(s) 929, 1034, 3202, 3357, 3765
PspGI CCWGG 5 cut(s) 929, 1034, 3202, 3357, 3765
PspN4I GGNNCC 8 cut(s) 123, 639, 798, 1875, 2034, 2673, 3364, 3380
PspPI GGNCC 5 cut(s) 837, 1394, 2461, 2912, 3379
PspPPI RGGWCCY 1 cut(s) 3379
PspXI VCTCGAGB 1 cut(s) 1191
PstNI CAGNNNCTG 1 cut(s) 88
PsuI RGATCY 4 cut(s) 1290, 1344, 1873, 3386
PsyI GACNNNGTC 1 cut(s) 3574
RsaI GTAC 9 cut(s) 87, 770, 893, 1033, 1121, 2673, 3099, 3643, 3708
RsaNI GTAC 9 cut(s) 86, 769, 892, 1032, 1120, 2672, 3098, 3642, 3707
RseI CAYNNNNRTG 4 cut(s) 2898, 3225, 3597, 3714
SapI GCTCTTC 1 cut(s) 682
SatI GCNGC 8 cut(s) 150, 519, 1353, 1362, 1491, 2116, 2790, 3515
Sau96I GGNCC 5 cut(s) 837, 1394, 2461, 2912, 3379
ScaI AGTACT 1 cut(s) 770
SchI GAGTC 2 cut(s) 203, 602
ScrFI CCNGG 8 cut(s) 23, 460, 461, 931, 1036, 3204, 3359, 3767
SduI GDGCHC 4 cut(s) 274, 1064, 2035, 2448
Sfr274I CTCGAG 1 cut(s) 1191
SinI GGWCC 4 cut(s) 1394, 2461, 2912, 3379
SlaI CTCGAG 1 cut(s) 1191
SmaI CCCGGG 1 cut(s) 461
SmiMI CAYNNNNRTG 4 cut(s) 2898, 3225, 3597, 3714
SmlI CTYRAG 9 cut(s) 1055, 1191, 1256, 1793, 1814, 2821, 3007, 3281, 3530
SmoI CTYRAG 9 cut(s) 1055, 1191, 1256, 1793, 1814, 2821, 3007, 3281, 3530
SpeI ACTAGT 2 cut(s) 3713, 3829
SseBI AGGCCT 3 cut(s) 1077, 1131, 2819
SsiI CCGC 4 cut(s) 149, 152, 471, 991
SspI AATATT 1 cut(s) 2867
StuI AGGCCT 3 cut(s) 1077, 1131, 2819
StyD4I CCNGG 8 cut(s) 21, 458, 459, 929, 1034, 3202, 3357, 3765
StyI CCWWGG 5 cut(s) 322, 558, 1286, 1390, 3382
TaiI ACGT 4 cut(s) 45, 1560, 2806, 2956
TatI WGTACW 3 cut(s) 768, 891, 3641
TauI GCSGC 1 cut(s) 152
TfiI GAWTC 8 cut(s) 262, 868, 934, 974, 1227, 2798, 3061, 3771
TscAI CASTG 8 cut(s) 262, 322, 648, 976, 1215, 2125, 3604, 3727
TseFI GTSAC 2 cut(s) 2194, 3716
TseI GCWGC 7 cut(s) 518, 1352, 1361, 1490, 2115, 2789, 3514
Tsp45I GTSAC 2 cut(s) 2194, 3716
TspGWI ACGGA 2 cut(s) 2664, 3449
TspMI CCCGGG 1 cut(s) 459
TspRI CASTG 8 cut(s) 262, 322, 648, 976, 1215, 2125, 3604, 3727
Tth111I GACNNNGTC 1 cut(s) 3574
Van91I CCANNNNNTGG 2 cut(s) 564, 930
Vha464I CTTAAG 2 cut(s) 1814, 3007
VpaK11BI GGWCC 4 cut(s) 1394, 2461, 2912, 3379
XagI CCTNNNNNAGG 1 cut(s) 3533
XbaI TCTAGA 1 cut(s) 3508
XceI RCATGY 5 cut(s) 65, 406, 2082, 2336, 2660
XcmI CCANNNNNNNNNTGG 1 cut(s) 1284
XhoI CTCGAG 1 cut(s) 1191
XmaI CCCGGG 1 cut(s) 459
XmaJI CCTAGG 1 cut(s) 3382
XmiI GTMKAC 1 cut(s) 2255
XmnI GAANNNNTTC 2 cut(s) 418, 2472
ZraI GACGTC 1 cut(s) 43
ZrmI AGTACT 1 cut(s) 770
Zsp2I ATGCAT 1 cut(s) 294
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.