MD12G1140300.v1.1

Fusaric acid resistance protein-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr12
OG0003101
Physical Location & Seq
Reverse (-)
21809176 .. 21810208
1033 bp
Loading structure...
UTR
Exon/CDS
Intron
MD12G1140300.v1.1.491

Sequence Viewer

Length: 954 bp
ATGGGGGATTACCAAGCGCAATCGCTGTGGCAGATGCGCCTAGGCTTCGCCCTTCGCACGGCTTTGGCATGCACCATTGTCGACTGCACCGTCCTCTATGGACCTCCACAACTTAAAAAGTACATAACCTACCCGTCCTTTTCGTACATGACCACAATCCTAATAGCCCCGGACGCAATGCTTGGGGACGTCATGAGAAGCTGTTGGCATGTGATATTTGCCACGACACAGGTACTTTTGTCTTCTGTGCTGACCCTTTGGCTGGTGGAACTCCTCCTAGAAAACCAACCCATTGCCCGGAATCCTGGAAACACTAGGAAACCTAACCAAGAACCGAGTGATTCGCAAAATCAACAGCGGTGGAACTTCAAAAGTGTTTGGAGAAACATAATCCCAAGTACCAGGAGTATAATTTTTGCTCTCAAGTGCTCACTTGCATTAGGCCTTGTTGTGTTATTCGGCTTGTTGTATAACAAGGAAAACGGGTACTGGTCAGGCCTCACCATTGCCATCAGCTTTGTAACAGGACGGCAAGCAACATTCACAGTTACAAATGCTCGAGTTCAAGGAACAGCACTGGGATCGATCTACGGAATCATATGGCATAGCAAGATGTATGGCCAAGCTGGTGGGATCTCGGCAGCCATTGCAACATTACTTATCTTGGGTAGGGAAAACTATGGTCCTCCAAGTGAATTTGCATTTGCAAGAATGATAGAGGCATCCATTAGACTGCTTTGTTTCCTTTTAGTAGAGATTGTCTTTTATCCGATGAGAGCAGCGACCCTAGCAAAAAATAAGCTCTCACAAAGCATGGAGGCGCTTCGAGATTGTATCGGAGTAAATCTTTGCGTACCGGCTTCCACAGGATTGAGGGATGATAGGGATTTTACTACTCATGTGAATAAGCTAAAAACTCCTATTATACTTCCAAGAATCACAAGGTTGTTGTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
FUSC FUSC_2

Protein Analysis

318

Amino Acids

35.35

Weight (kDa)

9.53

Isoelectric Point (pI)

42.69

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
FUSC_2 PF13515 150 - 221 7.6e-06 Fusaric acid resistance protein-like
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 89
AatII GACGTC 1 cut(s) 192
AccI GTMKAC 1 cut(s) 81
AciI CCGC 1 cut(s) 358
AclWI GGATC 2 cut(s) 589, 641
AcoI YGGCCR 1 cut(s) 619
AcsI RAATTY 1 cut(s) 695
AcyI GRCGYC 1 cut(s) 189
AfaI GTAC 6 cut(s) 122, 146, 234, 400, 488, 855
AfiI CCNNNNNNNGG 3 cut(s) 58, 262, 297
AgsI TTSAA 2 cut(s) 370, 566
AjnI CCWGG 2 cut(s) 304, 401
AluBI AGCT 5 cut(s) 201, 516, 626, 802, 910
AluI AGCT 5 cut(s) 201, 516, 626, 802, 910
Alw21I GWGCWC 1 cut(s) 431
AlwI GGATC 2 cut(s) 589, 641
Ama87I CYCGRG 1 cut(s) 558
AoxI GGCC 3 cut(s) 442, 496, 619
ApeKI GCWGC 2 cut(s) 641, 779
ApoI RAATTY 1 cut(s) 695
AspA2I CCTAGG 1 cut(s) 40
AspLEI GCGC 3 cut(s) 19, 39, 823
AspS9I GGNCC 2 cut(s) 101, 683
AsuC2I CCSGG 2 cut(s) 170, 298
AsuHPI GGTGA 1 cut(s) 493
AvaI CYCGRG 1 cut(s) 558
AvaII GGWCC 2 cut(s) 101, 683
AvrII CCTAGG 1 cut(s) 40
BaeI ACNNNNGTAYC 2 cut(s) 224, 257
BalI TGGCCA 1 cut(s) 621
BbsI GAAGAC 1 cut(s) 234
Bbv12I GWGCWC 1 cut(s) 431
BbvI GCAGC 2 cut(s) 653, 791
BccI CCATC 1 cut(s) 518
BceAI ACGGC 2 cut(s) 75, 545
BcgI CGANNNNNNTGC 4 cut(s) 61, 95, 564, 598
BciT130I CCWGG 2 cut(s) 306, 403
BcnI CCSGG 2 cut(s) 170, 298
BfaI CTAG 4 cut(s) 41, 278, 315, 788
BfoI RGCGCY 1 cut(s) 824
BisI GCNGC 2 cut(s) 642, 780
BlnI CCTAGG 1 cut(s) 40
BlsI GCNGC 2 cut(s) 643, 781
Bme1390I CCNGG 4 cut(s) 170, 298, 306, 403
Bme18I GGWCC 2 cut(s) 101, 683
BmeT110I CYCGRG 1 cut(s) 558
BmgT120I GGNCC 2 cut(s) 101, 683
BmrFI CCNGG 4 cut(s) 170, 298, 306, 403
BmrI ACTGGG 1 cut(s) 587
BmsI GCATC 2 cut(s) 24, 731
BmuI ACTGGG 1 cut(s) 587
BpiI GAAGAC 1 cut(s) 234
BpuEI CTTGAG 1 cut(s) 407
BpuMI CCSGG 2 cut(s) 170, 298
Bsa29I ATCGAT 1 cut(s) 584
BsaHI GRCGYC 1 cut(s) 189
BsaJI CCNNGG 2 cut(s) 40, 168
Bsc4I CCNNNNNNNGG 3 cut(s) 58, 262, 297
Bse118I RCCGGY 1 cut(s) 856
Bse1I ACTGG 2 cut(s) 494, 582
Bse3DI GCAATG 4 cut(s) 183, 291, 504, 645
BseBI CCWGG 2 cut(s) 306, 403
BseCI ATCGAT 1 cut(s) 584
BseDI CCNNGG 2 cut(s) 40, 168
BseGI GGATG 2 cut(s) 722, 883
BseLI CCNNNNNNNGG 3 cut(s) 58, 262, 297
BseMI GCAATG 4 cut(s) 183, 291, 504, 645
BseNI ACTGG 2 cut(s) 494, 582
BseRI GAGGAG 1 cut(s) 263
BseXI GCAGC 2 cut(s) 653, 791
BsgI GTGCAG 1 cut(s) 70
BshFI GGCC 3 cut(s) 444, 498, 621
BshVI ATCGAT 1 cut(s) 584
BsiHKAI GWGCWC 1 cut(s) 431
BsiHKCI CYCGRG 1 cut(s) 558
BsiSI CCGG 3 cut(s) 170, 298, 857
BslFI GGGAC 1 cut(s) 200
BslI CCNNNNNNNGG 3 cut(s) 58, 262, 297
BsmFI GGGAC 1 cut(s) 200
BsnI GGCC 3 cut(s) 444, 498, 621
BsoBI CYCGRG 1 cut(s) 558
Bsp1286I GDGCHC 1 cut(s) 431
Bsp143I GATC 3 cut(s) 581, 585, 633
BspACI CCGC 1 cut(s) 358
BspANI GGCC 3 cut(s) 444, 498, 621
BspDI ATCGAT 1 cut(s) 584
BspHI TCATGA 1 cut(s) 192
BspPI GGATC 2 cut(s) 589, 641
BsrDI GCAATG 4 cut(s) 183, 291, 504, 645
BsrFI RCCGGY 1 cut(s) 856
BsrI ACTGG 2 cut(s) 494, 582
BssAI RCCGGY 1 cut(s) 856
BssECI CCNNGG 2 cut(s) 40, 168
BssMI GATC 3 cut(s) 581, 585, 633
BssNI GRCGYC 1 cut(s) 189
BssT1I CCWWGG 1 cut(s) 40
Bst2UI CCWGG 2 cut(s) 306, 403
Bst4CI ACNGT 2 cut(s) 91, 547
BstACI GRCGYC 1 cut(s) 189
BstAPI GCANNNNNTGC 1 cut(s) 647
BstC8I GCNNGC 2 cut(s) 70, 534
BstF5I GGATG 2 cut(s) 722, 883
BstH2I RGCGCY 1 cut(s) 824
BstHHI GCGC 3 cut(s) 19, 39, 823
BstKTI GATC 3 cut(s) 584, 588, 636
BstMBI GATC 3 cut(s) 581, 585, 633
BstMWI GCNNNNNNNGC 3 cut(s) 173, 647, 788
BstNI CCWGG 2 cut(s) 306, 403
BstNSI RCATGY 2 cut(s) 72, 212
BstSCI CCNGG 4 cut(s) 168, 296, 304, 401
BstV1I GCAGC 2 cut(s) 653, 791
BstV2I GAAGAC 1 cut(s) 234
BstX2I RGATCY 1 cut(s) 633
BstXI CCANNNNNNTGG 1 cut(s) 629
BstYI RGATCY 1 cut(s) 633
Bsu15I ATCGAT 1 cut(s) 584
BsuRI GGCC 3 cut(s) 444, 498, 621
BsuTUI ATCGAT 1 cut(s) 584
BtsCI GGATG 2 cut(s) 722, 883
BtsIMutI CAGTG 1 cut(s) 575
Cac8I GCNNGC 2 cut(s) 70, 534
CciI TCATGA 1 cut(s) 192
CfoI GCGC 3 cut(s) 19, 39, 823
Cfr10I RCCGGY 1 cut(s) 856
Cfr13I GGNCC 2 cut(s) 101, 683
ClaI ATCGAT 1 cut(s) 584
CseI GACGC 1 cut(s) 182
Csp6I GTAC 6 cut(s) 121, 145, 233, 399, 487, 854
CspCI CAANNNNNGTGG 4 cut(s) 8, 43, 341, 376
CviAII CATG 6 cut(s) 69, 148, 193, 209, 814, 899
CviQI GTAC 6 cut(s) 121, 145, 233, 399, 487, 854
DpnI GATC 3 cut(s) 583, 587, 635
DpnII GATC 3 cut(s) 581, 585, 633
DrdI GACNNNNNNGTC 1 cut(s) 89
DseDI GACNNNNNNGTC 1 cut(s) 89
EaeI YGGCCR 1 cut(s) 619
Eco130I CCWWGG 1 cut(s) 40
Eco147I AGGCCT 2 cut(s) 444, 498
Eco47I GGWCC 2 cut(s) 101, 683
Eco88I CYCGRG 1 cut(s) 558
EcoRII CCWGG 2 cut(s) 304, 401
EcoT14I CCWWGG 1 cut(s) 40
ErhI CCWWGG 1 cut(s) 40
FaeI CATG 6 cut(s) 72, 151, 196, 212, 817, 902
FaqI GGGAC 1 cut(s) 200
FatI CATG 6 cut(s) 68, 147, 192, 208, 813, 898
FauNDI CATATG 1 cut(s) 599
FblI GTMKAC 1 cut(s) 81
Fnu4HI GCNGC 2 cut(s) 642, 780
FokI GGATG 2 cut(s) 709, 890
Fsp4HI GCNGC 2 cut(s) 642, 780
FspBI CTAG 4 cut(s) 41, 278, 315, 788
GlaI GCGC 3 cut(s) 18, 38, 822
GluI GCNGC 2 cut(s) 642, 780
HaeII RGCGCY 1 cut(s) 824
HaeIII GGCC 3 cut(s) 444, 498, 621
HapII CCGG 3 cut(s) 170, 298, 857
HgaI GACGC 1 cut(s) 182
HhaI GCGC 3 cut(s) 19, 39, 823
Hin1I GRCGYC 1 cut(s) 189
Hin1II CATG 6 cut(s) 72, 151, 196, 212, 817, 902
Hin6I GCGC 3 cut(s) 17, 37, 821
HinP1I GCGC 3 cut(s) 17, 37, 821
HincII GTYRAC 1 cut(s) 82
HindII GTYRAC 1 cut(s) 82
HinfI GANTC 4 cut(s) 301, 341, 594, 936
HpaII CCGG 3 cut(s) 170, 298, 857
HphI GGTGA 1 cut(s) 493
Hpy166II GTNNAC 1 cut(s) 82
Hpy188I TCNGA 2 cut(s) 771, 839
Hpy188III TCNNGA 2 cut(s) 193, 827
Hpy8I GTNNAC 1 cut(s) 82
HpyAV CCTTC 1 cut(s) 62
HpyCH4III ACNGT 2 cut(s) 91, 547
HpyCH4IV ACGT 1 cut(s) 189
HpyCH4V TGCA 6 cut(s) 72, 87, 437, 650, 701, 707
HpyF10VI GCNNNNNNNGC 3 cut(s) 173, 647, 788
HpySE526I ACGT 1 cut(s) 189
Hsp92I GRCGYC 1 cut(s) 189
Hsp92II CATG 6 cut(s) 72, 151, 196, 212, 817, 902
HspAI GCGC 3 cut(s) 17, 37, 821
Kzo9I GATC 3 cut(s) 581, 585, 633
Lsp1109I GCAGC 2 cut(s) 653, 791
LweI GCATC 2 cut(s) 24, 731
MaeI CTAG 4 cut(s) 41, 278, 315, 788
MaeII ACGT 1 cut(s) 189
MaeIII GTNAC 2 cut(s) 520, 547
MalI GATC 3 cut(s) 583, 587, 635
MboI GATC 3 cut(s) 581, 585, 633
MboII GAAGA 1 cut(s) 234
MflI RGATCY 1 cut(s) 633
MhlI GDGCHC 1 cut(s) 431
MlsI TGGCCA 1 cut(s) 621
MluCI AATT 2 cut(s) 411, 695
MluNI TGGCCA 1 cut(s) 621
MnlI CCTC 8 cut(s) 104, 114, 284, 509, 696, 712, 811, 867
Mox20I TGGCCA 1 cut(s) 621
MscI TGGCCA 1 cut(s) 621
MseI TTAA 1 cut(s) 114
Msp20I TGGCCA 1 cut(s) 621
MspA1I CMGCKG 1 cut(s) 358
MspI CCGG 3 cut(s) 170, 298, 857
MspR9I CCNGG 4 cut(s) 170, 298, 306, 403
MvaI CCWGG 2 cut(s) 306, 403
MwoI GCNNNNNNNGC 3 cut(s) 173, 647, 788
NciI CCSGG 2 cut(s) 170, 298
NdeI CATATG 1 cut(s) 599
NdeII GATC 3 cut(s) 581, 585, 633
NlaIII CATG 6 cut(s) 72, 151, 196, 212, 817, 902
NmeAIII GCCGAG 1 cut(s) 617
NspI RCATGY 2 cut(s) 72, 212
PaeI GCATGC 1 cut(s) 72
PaeR7I CTCGAG 1 cut(s) 558
PagI TCATGA 1 cut(s) 192
PceI AGGCCT 2 cut(s) 444, 498
PcsI WCGNNNNNNNCGW 1 cut(s) 87
PfeI GAWTC 4 cut(s) 301, 341, 594, 936
PfoI TCCNGGA 1 cut(s) 304
PkrI GCNGC 2 cut(s) 643, 781
Psp6I CCWGG 2 cut(s) 304, 401
PspGI CCWGG 2 cut(s) 304, 401
PspPI GGNCC 2 cut(s) 101, 683
PspXI VCTCGAGB 1 cut(s) 558
PsuI RGATCY 1 cut(s) 633
RsaI GTAC 6 cut(s) 122, 146, 234, 400, 488, 855
RsaNI GTAC 6 cut(s) 121, 145, 233, 399, 487, 854
SalI GTCGAC 1 cut(s) 80
SaqAI TTAA 1 cut(s) 114
SatI GCNGC 2 cut(s) 642, 780
Sau3AI GATC 3 cut(s) 581, 585, 633
Sau96I GGNCC 2 cut(s) 101, 683
ScrFI CCNGG 4 cut(s) 170, 298, 306, 403
SduI GDGCHC 1 cut(s) 431
SfaNI GCATC 2 cut(s) 24, 731
Sfr274I CTCGAG 1 cut(s) 558
SinI GGWCC 2 cut(s) 101, 683
SlaI CTCGAG 1 cut(s) 558
SmlI CTYRAG 2 cut(s) 422, 558
SmoI CTYRAG 2 cut(s) 422, 558
SphI GCATGC 1 cut(s) 72
Sse9I AATT 2 cut(s) 411, 695
SseBI AGGCCT 2 cut(s) 444, 498
SsiI CCGC 1 cut(s) 358
SspMI CTAG 4 cut(s) 41, 278, 315, 788
StuI AGGCCT 2 cut(s) 444, 498
StyD4I CCNGG 4 cut(s) 168, 296, 304, 401
StyI CCWWGG 1 cut(s) 40
TaaI ACNGT 2 cut(s) 91, 547
TaiI ACGT 1 cut(s) 192
TaqI TCGA 4 cut(s) 81, 559, 584, 826
TasI AATT 2 cut(s) 411, 695
TatI WGTACW 1 cut(s) 120
TfiI GAWTC 4 cut(s) 301, 341, 594, 936
Tru1I TTAA 1 cut(s) 114
Tru9I TTAA 1 cut(s) 114
TscAI CASTG 1 cut(s) 582
TseI GCWGC 2 cut(s) 641, 779
TspGWI ACGGA 1 cut(s) 606
TspRI CASTG 1 cut(s) 582
VpaK11BI GGWCC 2 cut(s) 101, 683
XapI RAATTY 1 cut(s) 695
XceI RCATGY 2 cut(s) 72, 212
XhoI CTCGAG 1 cut(s) 558
XmaJI CCTAGG 1 cut(s) 40
XmiI GTMKAC 1 cut(s) 81
XspI CTAG 4 cut(s) 41, 278, 315, 788
ZraI GACGTC 1 cut(s) 190
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.