MD04G1136600.v1.1

May be involved in both secretory and endocytic intracellular trafficking in the endosomal prevacuolar compartments

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr04
OG0011985
Physical Location & Seq
Reverse (-)
22422574 .. 22425588
3015 bp
Loading structure...
UTR
Exon/CDS
Intron
MD04G1136600.v1.1.491

Sequence Viewer

Length: 594 bp
ATGGATTGGGGCAGCGTCACGGCCGAGGATGTCATCGACGCCCTCCGCGAAGTCGACTGGTCGTCTCCGCCGCGCCCTCTCTCCGAATTCTTCTCCAGGTTCACCGTCCCCAGATCCTCTTCCAAATGGAATAGCCGCCTCAAATGCAATCTCTACTACTACAGGACCAACTACTTCCTTTTGATCGTGTTAGTTCTTGGCTTGGCTTTTCTCCGGAGGCCACTCGCTCTCATCGCAGCTGTTCTTACCGCGCTAAGCATTGCTTTCCTCAATGATAGCTTTGCAGGTACCTTTAGTGAGAAAGTATCGAGAACTGTGAGACAATTCTCTCCACATTTAGCTGCAAAGATGAGACCTCCTCTTACCCCTGTAATTCGTGGACGTCCATCTGCTAAAAGAGCAATATTCATATGTGGACGGCCTCGATGGGTGTTTGTCATGATATTCTCTTCTGGTACTGAGTTTCATTCTATGGTTCGTCTCCTGTGGTCTCTTAACTGTATTGTGGGCAGTCACTTTTGGACTTCTTGCCACTTTGTTGCATGCAAGCTTCAGAACGCCAAACTTGAAAGCACGCCTGAACACTTACCGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005783 GO:0006810 GO:0008150 GO:0012505 GO:0016192 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044424 GO:0044444 GO:0044464 GO:0051179 GO:0051234
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
C1_2 PRA1

Protein Analysis

198

Amino Acids

22.46

Weight (kDa)

10.1

Isoelectric Point (pI)

51.61

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PRA1 PF03208 20 - 112 6.8e-24 PRA1 family protein
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0011985)

Species Orthologous Gene IDs
arabidopsis_thaliana AT3G11397 AT5G02040 AT5G02040 AT5G05987
fragaria_vesca FvH4_6g12950
malus_domestica MD04G1136600.v1.1 MD12G1150900.v1.1
prunus_persica Prupe.6G262800_v2.0.a1 Prupe.6G262800_v2.0.a1
rosa_chinensis RchiOBHm_Chr3g0465061
rosa_laevigata RLG00000024659
rosa_multiflora Rmu_sc0004362.1_g000018
rosa_roxburghii Rroxscaffold_6G00415290
rosa_rugosa Rorug03G0075000
rosa_samantha Rh3BG138500 Rh3CG140900 Rh3CG149200 Rh3DG140000
rosa_wichuraiana Rw3G011330

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 385
Acc36I ACCTGC 1 cut(s) 275
Acc65I GGTACC 1 cut(s) 287
AccB1I GGYRCC 1 cut(s) 287
AccI GTMKAC 1 cut(s) 54
AccII CGCG 3 cut(s) 48, 73, 251
AccIII TCCGGA 1 cut(s) 213
AciI CCGC 5 cut(s) 46, 68, 71, 136, 249
AclWI GGATC 1 cut(s) 108
AcoI YGGCCR 1 cut(s) 21
AcsI RAATTY 1 cut(s) 86
AcuI CTGAAG 1 cut(s) 536
AcyI GRCGYC 2 cut(s) 39, 382
AfaI GTAC 2 cut(s) 289, 457
AgsI TTSAA 1 cut(s) 569
AhdI GACNNNNNGTC 1 cut(s) 61
AjnI CCWGG 1 cut(s) 95
AluBI AGCT 4 cut(s) 239, 279, 341, 550
AluI AGCT 4 cut(s) 239, 279, 341, 550
Alw26I GTCTC 5 cut(s) 69, 313, 346, 485, 495
AlwI GGATC 1 cut(s) 108
Aor13HI TCCGGA 1 cut(s) 213
AoxI GGCC 3 cut(s) 21, 218, 419
ApeKI GCWGC 3 cut(s) 12, 236, 341
ApoI RAATTY 1 cut(s) 86
Asp718I GGTACC 1 cut(s) 287
AspLEI GCGC 2 cut(s) 75, 253
AspS9I GGNCC 1 cut(s) 165
AsuHPI GGTGA 1 cut(s) 94
AvaII GGWCC 1 cut(s) 165
BanI GGYRCC 1 cut(s) 287
BbvI GCAGC 3 cut(s) 24, 248, 328
BccI CCATC 2 cut(s) 394, 420
BceAI ACGGC 2 cut(s) 36, 434
BciT130I CCWGG 1 cut(s) 97
BcoDI GTCTC 5 cut(s) 69, 313, 346, 485, 495
BfmI CTRYAG 1 cut(s) 160
BfuAI ACCTGC 1 cut(s) 275
BisI GCNGC 5 cut(s) 13, 71, 136, 237, 342
BlpI GCTNAGC 1 cut(s) 254
BlsI GCNGC 5 cut(s) 14, 72, 137, 238, 343
Bme1390I CCNGG 1 cut(s) 97
Bme18I GGWCC 1 cut(s) 165
BmeRI GACNNNNNGTC 1 cut(s) 61
BmgT120I GGNCC 1 cut(s) 165
BmiI GGNNCC 1 cut(s) 289
BmrFI CCNGG 1 cut(s) 97
BplI GAGNNNNNCTC 2 cut(s) 343, 375
BpmI CTGGAG 1 cut(s) 79
Bpu1102I GCTNAGC 1 cut(s) 254
BsaHI GRCGYC 2 cut(s) 39, 382
BsaI GGTCTC 2 cut(s) 346, 495
BsaJI CCNNGG 1 cut(s) 24
BsaWI WCCGGW 1 cut(s) 213
Bse1I ACTGG 1 cut(s) 62
Bse3DI GCAATG 1 cut(s) 258
BseAI TCCGGA 1 cut(s) 213
BseBI CCWGG 1 cut(s) 97
BseDI CCNNGG 1 cut(s) 24
BseGI GGATG 1 cut(s) 34
BseMI GCAATG 1 cut(s) 258
BseMII CTCAG 1 cut(s) 450
BseNI ACTGG 1 cut(s) 62
BseRI GAGGAG 1 cut(s) 348
BseX3I CGGCCG 1 cut(s) 21
BseXI GCAGC 3 cut(s) 24, 248, 328
Bsh1236I CGCG 3 cut(s) 48, 73, 251
Bsh1285I CGRYCG 1 cut(s) 24
BshFI GGCC 3 cut(s) 23, 220, 421
BshNI GGYRCC 1 cut(s) 287
BsiEI CGRYCG 1 cut(s) 24
BsiSI CCGG 1 cut(s) 214
BslFI GGGAC 1 cut(s) 92
BsmAI GTCTC 5 cut(s) 69, 313, 346, 485, 495
BsmBI CGTCTC 2 cut(s) 69, 485
BsmFI GGGAC 1 cut(s) 92
BsnI GGCC 3 cut(s) 23, 220, 421
Bso31I GGTCTC 2 cut(s) 346, 495
Bsp13I TCCGGA 1 cut(s) 213
Bsp143I GATC 2 cut(s) 113, 183
Bsp1720I GCTNAGC 1 cut(s) 254
BspACI CCGC 5 cut(s) 46, 68, 71, 136, 249
BspANI GGCC 3 cut(s) 23, 220, 421
BspCNI CTCAG 1 cut(s) 451
BspEI TCCGGA 1 cut(s) 213
BspFNI CGCG 3 cut(s) 48, 73, 251
BspHI TCATGA 1 cut(s) 438
BspLI GGNNCC 1 cut(s) 289
BspMI ACCTGC 1 cut(s) 275
BspPI GGATC 1 cut(s) 108
BspT107I GGYRCC 1 cut(s) 287
BspTNI GGTCTC 2 cut(s) 346, 495
BsrDI GCAATG 1 cut(s) 258
BsrI ACTGG 1 cut(s) 62
BssECI CCNNGG 1 cut(s) 24
BssMI GATC 2 cut(s) 113, 183
BssNI GRCGYC 2 cut(s) 39, 382
Bst2UI CCWGG 1 cut(s) 97
Bst4CI ACNGT 4 cut(s) 106, 316, 500, 591
Bst6I CTCTTC 2 cut(s) 124, 454
BstACI GRCGYC 2 cut(s) 39, 382
BstC8I GCNNGC 3 cut(s) 544, 548, 575
BstDEI CTNAG 2 cut(s) 254, 459
BstF5I GGATG 1 cut(s) 34
BstFNI CGCG 3 cut(s) 48, 73, 251
BstHHI GCGC 2 cut(s) 75, 253
BstKTI GATC 2 cut(s) 116, 186
BstMAI GTCTC 5 cut(s) 69, 313, 346, 485, 495
BstMBI GATC 2 cut(s) 113, 183
BstMCI CGRYCG 1 cut(s) 24
BstMWI GCNNNNNNNGC 3 cut(s) 144, 233, 398
BstNI CCWGG 1 cut(s) 97
BstNSI RCATGY 1 cut(s) 546
BstSCI CCNGG 1 cut(s) 95
BstSFI CTRYAG 1 cut(s) 160
BstUI CGCG 3 cut(s) 48, 73, 251
BstV1I GCAGC 3 cut(s) 24, 248, 328
BstX2I RGATCY 1 cut(s) 113
BstYI RGATCY 1 cut(s) 113
BstZI CGGCCG 1 cut(s) 21
BsuRI GGCC 3 cut(s) 23, 220, 421
BtgZI GCGATG 1 cut(s) 217
BtsCI GGATG 1 cut(s) 34
BveI ACCTGC 1 cut(s) 275
Cac8I GCNNGC 3 cut(s) 544, 548, 575
CciI TCATGA 1 cut(s) 438
CfoI GCGC 2 cut(s) 75, 253
Cfr13I GGNCC 1 cut(s) 165
CseI GACGC 2 cut(s) 4, 47
Csp6I GTAC 2 cut(s) 288, 456
CviAII CATG 2 cut(s) 439, 543
CviQI GTAC 2 cut(s) 288, 456
DdeI CTNAG 2 cut(s) 254, 459
DpnI GATC 2 cut(s) 115, 185
DpnII GATC 2 cut(s) 113, 183
DriI GACNNNNNGTC 1 cut(s) 61
EaeI YGGCCR 1 cut(s) 21
EagI CGGCCG 1 cut(s) 21
Eam1104I CTCTTC 2 cut(s) 124, 454
Eam1105I GACNNNNNGTC 1 cut(s) 61
EarI CTCTTC 2 cut(s) 124, 454
EciI GGCGGA 1 cut(s) 57
EclXI CGGCCG 1 cut(s) 21
Eco31I GGTCTC 2 cut(s) 346, 495
Eco47I GGWCC 1 cut(s) 165
Eco52I CGGCCG 1 cut(s) 21
Eco57I CTGAAG 1 cut(s) 536
EcoRI GAATTC 1 cut(s) 86
EcoRII CCWGG 1 cut(s) 95
Esp3I CGTCTC 2 cut(s) 69, 485
FaeI CATG 2 cut(s) 442, 546
FaiI YATR 5 cut(s) 410, 412, 440, 473, 544
FalI AAGNNNNNCTT 2 cut(s) 247, 279
FaqI GGGAC 1 cut(s) 92
FatI CATG 2 cut(s) 438, 542
FauNDI CATATG 1 cut(s) 410
FblI GTMKAC 1 cut(s) 54
Fnu4HI GCNGC 5 cut(s) 13, 71, 136, 237, 342
FokI GGATG 1 cut(s) 41
Fsp4HI GCNGC 5 cut(s) 13, 71, 136, 237, 342
GlaI GCGC 2 cut(s) 74, 252
GluI GCNGC 5 cut(s) 13, 71, 136, 237, 342
GsuI CTGGAG 1 cut(s) 79
HaeIII GGCC 3 cut(s) 23, 220, 421
HapII CCGG 1 cut(s) 214
HgaI GACGC 2 cut(s) 4, 47
HhaI GCGC 2 cut(s) 75, 253
Hin1I GRCGYC 2 cut(s) 39, 382
Hin1II CATG 2 cut(s) 442, 546
Hin6I GCGC 2 cut(s) 73, 251
HinP1I GCGC 2 cut(s) 73, 251
HincII GTYRAC 1 cut(s) 55
HindII GTYRAC 1 cut(s) 55
HindIII AAGCTT 1 cut(s) 548
HpaII CCGG 1 cut(s) 214
HphI GGTGA 1 cut(s) 94
Hpy166II GTNNAC 4 cut(s) 55, 102, 380, 416
Hpy188I TCNGA 2 cut(s) 85, 555
Hpy188III TCNNGA 3 cut(s) 214, 309, 439
Hpy8I GTNNAC 4 cut(s) 55, 102, 380, 416
Hpy99I CGWCG 1 cut(s) 41
HpyCH4III ACNGT 4 cut(s) 106, 316, 500, 591
HpyCH4IV ACGT 1 cut(s) 382
HpyCH4V TGCA 5 cut(s) 147, 284, 344, 542, 546
HpyF10VI GCNNNNNNNGC 3 cut(s) 144, 233, 398
HpyF3I CTNAG 2 cut(s) 254, 459
HpySE526I ACGT 1 cut(s) 382
Hsp92I GRCGYC 2 cut(s) 39, 382
Hsp92II CATG 2 cut(s) 442, 546
HspAI GCGC 2 cut(s) 73, 251
Kpn2I TCCGGA 1 cut(s) 213
KpnI GGTACC 1 cut(s) 291
Kzo9I GATC 2 cut(s) 113, 183
Lsp1109I GCAGC 3 cut(s) 24, 248, 328
MaeII ACGT 1 cut(s) 382
MaeIII GTNAC 2 cut(s) 16, 512
MalI GATC 2 cut(s) 115, 185
MboI GATC 2 cut(s) 113, 183
MboII GAAGA 3 cut(s) 82, 111, 441
MflI RGATCY 1 cut(s) 113
MluCI AATT 3 cut(s) 86, 323, 372
MroI TCCGGA 1 cut(s) 213
MseI TTAA 1 cut(s) 495
MspA1I CMGCKG 1 cut(s) 239
MspI CCGG 1 cut(s) 214
MspR9I CCNGG 1 cut(s) 97
MvaI CCWGG 1 cut(s) 97
MvnI CGCG 3 cut(s) 48, 73, 251
MwoI GCNNNNNNNGC 3 cut(s) 144, 233, 398
NdeI CATATG 1 cut(s) 410
NdeII GATC 2 cut(s) 113, 183
NlaIII CATG 2 cut(s) 442, 546
NlaIV GGNNCC 1 cut(s) 289
NmeAIII GCCGAG 1 cut(s) 49
NmuCI GTSAC 2 cut(s) 16, 512
NspI RCATGY 1 cut(s) 546
PaeI GCATGC 1 cut(s) 546
PagI TCATGA 1 cut(s) 438
PcsI WCGNNNNNNNCGW 1 cut(s) 45
PkrI GCNGC 5 cut(s) 14, 72, 137, 238, 343
Psp6I CCWGG 1 cut(s) 95
PspGI CCWGG 1 cut(s) 95
PspN4I GGNNCC 1 cut(s) 289
PspPI GGNCC 1 cut(s) 165
PsuI RGATCY 1 cut(s) 113
PvuII CAGCTG 1 cut(s) 239
RsaI GTAC 2 cut(s) 289, 457
RsaNI GTAC 2 cut(s) 288, 456
SalI GTCGAC 1 cut(s) 53
SaqAI TTAA 1 cut(s) 495
SatI GCNGC 5 cut(s) 13, 71, 136, 237, 342
Sau3AI GATC 2 cut(s) 113, 183
Sau96I GGNCC 1 cut(s) 165
ScrFI CCNGG 1 cut(s) 97
SetI ASST 9 cut(s) 101, 241, 281, 289, 293, 343, 358, 385, 552
SfcI CTRYAG 1 cut(s) 160
SinI GGWCC 1 cut(s) 165
SphI GCATGC 1 cut(s) 546
Sse9I AATT 3 cut(s) 86, 323, 372
SsiI CCGC 5 cut(s) 46, 68, 71, 136, 249
SspI AATATT 1 cut(s) 405
StyD4I CCNGG 1 cut(s) 95
TaaI ACNGT 4 cut(s) 106, 316, 500, 591
TaiI ACGT 1 cut(s) 385
TaqI TCGA 4 cut(s) 36, 54, 308, 424
TasI AATT 3 cut(s) 86, 323, 372
TauI GCSGC 2 cut(s) 73, 138
Tru1I TTAA 1 cut(s) 495
Tru9I TTAA 1 cut(s) 495
TseFI GTSAC 2 cut(s) 16, 512
TseI GCWGC 3 cut(s) 12, 236, 341
Tsp45I GTSAC 2 cut(s) 16, 512
TspDTI ATGAA 2 cut(s) 397, 455
VpaK11BI GGWCC 1 cut(s) 165
XapI RAATTY 1 cut(s) 86
XceI RCATGY 1 cut(s) 546
XmiI GTMKAC 1 cut(s) 54
ZraI GACGTC 1 cut(s) 383
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.