MD12G1150900.v1.1

May be involved in both secretory and endocytic intracellular trafficking in the endosomal prevacuolar compartments

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr12
OG0011985
Physical Location & Seq
Reverse (-)
23100166 .. 23102800
2635 bp
Loading structure...
UTR
Exon/CDS
Intron
MD12G1150900.v1.1.491

Sequence Viewer

Length: 726 bp
ATGGATTGGGGCAACGTCACGGCGGAGGATGTGATCGACGCCCTCCGCGAGGTCGACTGGTCGTCTCCGCCGCGCCCTCTCTCTGAATTCTTCTCCAGGTTCACCATCCCCAGATCCTCCTCCAAATGGAATAGCCGCCTCAAATGCAATCTTTACTACTACAGGACCAACTACTTCCTTTTGATCGTGCTAGTTCTTGGCTTGGCTTTTCTCCGGAGTCCACTCGCTCTTATTGCGGCTGTTCTTACTGCGCTCAGCATTGCGTTTCTCAATGACAGCTTTGCAGGTACCTTTAGTGAGAAAGTTTCAAGAACCGTGAGACAATTCTCTCCACATTTAGCTGCAAAAATGAGACCTCCCCTTACGCCTGTAATTCGTGGACGTCCATCAGCTAAAAGAGCAATATTCATATGTGGTCGGCCTCGATGGGTGTTTGTCATGATATTCTCTTCTGTGAGTTTCATTCTATGGTTTGTCTCCTGTGGTCTCTTAACTGTGTTGTGGGCACTTGCTTTTGGACTTCTTGCCACTTTGTTGCACGCAAGCTTCAGAACACCAAACTTGAAAGCACGCCTGAACACTTACCGTGAGGAATTTCGTGCAGTTTGGCGCAACTATAGTGAGCTCCTTTTATTGTTTCTATTTAGACATTGTCCTGTTGATTGTGAATCTCATACAAGATATGTGATTCTAAACTTGAGCTTCGCTGTGGCTGCCTTCAAATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005783 GO:0006810 GO:0008150 GO:0012505 GO:0016192 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044424 GO:0044444 GO:0044464 GO:0051179 GO:0051234
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
C1_2 PRA1

Protein Analysis

242

Amino Acids

27.66

Weight (kDa)

10.24

Isoelectric Point (pI)

43.78

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PRA1 PF03208 20 - 192 1.1e-37 PRA1 family protein
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0011985)

Species Orthologous Gene IDs
arabidopsis_thaliana AT3G11397 AT5G02040 AT5G02040 AT5G05987
fragaria_vesca FvH4_6g12950
malus_domestica MD04G1136600.v1.1 MD12G1150900.v1.1
prunus_persica Prupe.6G262800_v2.0.a1 Prupe.6G262800_v2.0.a1
rosa_chinensis RchiOBHm_Chr3g0465061
rosa_laevigata RLG00000024659
rosa_multiflora Rmu_sc0004362.1_g000018
rosa_roxburghii Rroxscaffold_6G00415290
rosa_rugosa Rorug03G0075000
rosa_samantha Rh3BG138500 Rh3CG140900 Rh3CG149200 Rh3DG140000
rosa_wichuraiana Rw3G011330

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 385
Acc36I ACCTGC 1 cut(s) 275
Acc65I GGTACC 1 cut(s) 287
AccB1I GGYRCC 1 cut(s) 287
AccI GTMKAC 1 cut(s) 54
AccII CGCG 2 cut(s) 48, 73
AccIII TCCGGA 1 cut(s) 213
AciI CCGC 6 cut(s) 23, 46, 68, 71, 136, 236
AclWI GGATC 1 cut(s) 108
AcsI RAATTY 2 cut(s) 86, 593
AcuI CTGAAG 1 cut(s) 532
AcyI GRCGYC 2 cut(s) 39, 382
AfaI GTAC 1 cut(s) 289
AfiI CCNNNNNNNGG 2 cut(s) 49, 126
AgsI TTSAA 3 cut(s) 309, 565, 721
AhdI GACNNNNNGTC 1 cut(s) 61
AjnI CCWGG 1 cut(s) 95
AluBI AGCT 6 cut(s) 279, 341, 392, 546, 625, 702
AluI AGCT 6 cut(s) 279, 341, 392, 546, 625, 702
Alw21I GWGCWC 1 cut(s) 627
Alw26I GTCTC 5 cut(s) 69, 313, 346, 481, 491
AlwI GGATC 1 cut(s) 108
Aor13HI TCCGGA 1 cut(s) 213
AoxI GGCC 1 cut(s) 419
ApeKI GCWGC 2 cut(s) 341, 713
ApoI RAATTY 2 cut(s) 86, 593
Asp718I GGTACC 1 cut(s) 287
AspLEI GCGC 3 cut(s) 75, 253, 612
AspS9I GGNCC 1 cut(s) 165
AsuHPI GGTGA 1 cut(s) 94
AvaII GGWCC 1 cut(s) 165
BaeGI GKGCMC 1 cut(s) 508
BanI GGYRCC 1 cut(s) 287
BanII GRGCYC 1 cut(s) 627
Bbv12I GWGCWC 1 cut(s) 627
BbvI GCAGC 2 cut(s) 328, 700
BccI CCATC 3 cut(s) 113, 394, 420
BceAI ACGGC 1 cut(s) 36
BciT130I CCWGG 1 cut(s) 97
BcoDI GTCTC 5 cut(s) 69, 313, 346, 481, 491
BfaI CTAG 1 cut(s) 191
BfmI CTRYAG 2 cut(s) 160, 616
BfuAI ACCTGC 1 cut(s) 275
BisI GCNGC 5 cut(s) 71, 136, 237, 342, 714
BlpI GCTNAGC 1 cut(s) 254
BlsI GCNGC 5 cut(s) 72, 137, 238, 343, 715
Bme1390I CCNGG 1 cut(s) 97
Bme18I GGWCC 1 cut(s) 165
BmeRI GACNNNNNGTC 1 cut(s) 61
BmgT120I GGNCC 1 cut(s) 165
BmiI GGNNCC 1 cut(s) 289
BmrFI CCNGG 1 cut(s) 97
BpmI CTGGAG 1 cut(s) 79
Bpu1102I GCTNAGC 1 cut(s) 254
BpuEI CTTGAG 1 cut(s) 718
BsaHI GRCGYC 2 cut(s) 39, 382
BsaI GGTCTC 2 cut(s) 346, 491
BsaWI WCCGGW 1 cut(s) 213
Bsc4I CCNNNNNNNGG 2 cut(s) 49, 126
Bse1I ACTGG 1 cut(s) 62
Bse3DI GCAATG 1 cut(s) 258
BseAI TCCGGA 1 cut(s) 213
BseBI CCWGG 1 cut(s) 97
BseGI GGATG 2 cut(s) 34, 105
BseLI CCNNNNNNNGG 2 cut(s) 49, 126
BseMI GCAATG 1 cut(s) 258
BseMII CTCAG 1 cut(s) 268
BseNI ACTGG 1 cut(s) 62
BseRI GAGGAG 1 cut(s) 109
BseSI GKGCMC 1 cut(s) 508
BseXI GCAGC 2 cut(s) 328, 700
BsgI GTGCAG 1 cut(s) 621
Bsh1236I CGCG 2 cut(s) 48, 73
BshFI GGCC 1 cut(s) 421
BshNI GGYRCC 1 cut(s) 287
BsiHKAI GWGCWC 1 cut(s) 627
BsiSI CCGG 1 cut(s) 214
BslI CCNNNNNNNGG 2 cut(s) 49, 126
BsmAI GTCTC 5 cut(s) 69, 313, 346, 481, 491
BsmBI CGTCTC 1 cut(s) 69
BsnI GGCC 1 cut(s) 421
Bso31I GGTCTC 2 cut(s) 346, 491
Bsp1286I GDGCHC 2 cut(s) 508, 627
Bsp13I TCCGGA 1 cut(s) 213
Bsp143I GATC 3 cut(s) 33, 113, 183
Bsp1720I GCTNAGC 1 cut(s) 254
BspACI CCGC 6 cut(s) 23, 46, 68, 71, 136, 236
BspANI GGCC 1 cut(s) 421
BspCNI CTCAG 1 cut(s) 267
BspEI TCCGGA 1 cut(s) 213
BspFNI CGCG 2 cut(s) 48, 73
BspHI TCATGA 1 cut(s) 438
BspLI GGNNCC 1 cut(s) 289
BspMI ACCTGC 1 cut(s) 275
BspPI GGATC 1 cut(s) 108
BspT107I GGYRCC 1 cut(s) 287
BspTNI GGTCTC 2 cut(s) 346, 491
BsrDI GCAATG 1 cut(s) 258
BsrI ACTGG 1 cut(s) 62
BssMI GATC 3 cut(s) 33, 113, 183
BssNI GRCGYC 2 cut(s) 39, 382
Bst2UI CCWGG 1 cut(s) 97
Bst4CI ACNGT 3 cut(s) 316, 496, 587
Bst6I CTCTTC 1 cut(s) 454
BstACI GRCGYC 2 cut(s) 39, 382
BstC8I GCNNGC 3 cut(s) 540, 544, 571
BstDEI CTNAG 1 cut(s) 254
BstENI CCTNNNNNAGG 1 cut(s) 47
BstF5I GGATG 2 cut(s) 34, 105
BstFNI CGCG 2 cut(s) 48, 73
BstHHI GCGC 3 cut(s) 75, 253, 612
BstKTI GATC 3 cut(s) 36, 116, 186
BstMAI GTCTC 5 cut(s) 69, 313, 346, 481, 491
BstMBI GATC 3 cut(s) 33, 113, 183
BstMWI GCNNNNNNNGC 4 cut(s) 144, 233, 398, 713
BstNI CCWGG 1 cut(s) 97
BstSCI CCNGG 1 cut(s) 95
BstSFI CTRYAG 2 cut(s) 160, 616
BstSLI GKGCMC 1 cut(s) 508
BstUI CGCG 2 cut(s) 48, 73
BstV1I GCAGC 2 cut(s) 328, 700
BstX2I RGATCY 1 cut(s) 113
BstYI RGATCY 1 cut(s) 113
BsuRI GGCC 1 cut(s) 421
BtsCI GGATG 2 cut(s) 34, 105
BveI ACCTGC 1 cut(s) 275
Cac8I GCNNGC 3 cut(s) 540, 544, 571
CciI TCATGA 1 cut(s) 438
CfoI GCGC 3 cut(s) 75, 253, 612
Cfr13I GGNCC 1 cut(s) 165
CseI GACGC 1 cut(s) 47
Csp6I GTAC 1 cut(s) 288
CviAII CATG 1 cut(s) 439
CviQI GTAC 1 cut(s) 288
DdeI CTNAG 1 cut(s) 254
DpnI GATC 3 cut(s) 35, 115, 185
DpnII GATC 3 cut(s) 33, 113, 183
DriI GACNNNNNGTC 1 cut(s) 61
Eam1104I CTCTTC 1 cut(s) 454
Eam1105I GACNNNNNGTC 1 cut(s) 61
EarI CTCTTC 1 cut(s) 454
EciI GGCGGA 2 cut(s) 38, 57
Ecl136II GAGCTC 1 cut(s) 625
Eco24I GRGCYC 1 cut(s) 627
Eco31I GGTCTC 2 cut(s) 346, 491
Eco47I GGWCC 1 cut(s) 165
Eco53kI GAGCTC 1 cut(s) 625
Eco57I CTGAAG 1 cut(s) 532
EcoICRI GAGCTC 1 cut(s) 625
EcoNI CCTNNNNNAGG 1 cut(s) 47
EcoRI GAATTC 1 cut(s) 86
EcoRII CCWGG 1 cut(s) 95
EcoT38I GRGCYC 1 cut(s) 627
Esp3I CGTCTC 1 cut(s) 69
FaeI CATG 1 cut(s) 442
FaiI YATR 7 cut(s) 410, 412, 440, 469, 618, 675, 684
FatI CATG 1 cut(s) 438
FauNDI CATATG 1 cut(s) 410
FblI GTMKAC 1 cut(s) 54
Fnu4HI GCNGC 5 cut(s) 71, 136, 237, 342, 714
FokI GGATG 2 cut(s) 41, 92
FriOI GRGCYC 1 cut(s) 627
Fsp4HI GCNGC 5 cut(s) 71, 136, 237, 342, 714
FspBI CTAG 1 cut(s) 191
GlaI GCGC 3 cut(s) 74, 252, 611
GluI GCNGC 5 cut(s) 71, 136, 237, 342, 714
GsuI CTGGAG 1 cut(s) 79
HaeIII GGCC 1 cut(s) 421
HapII CCGG 1 cut(s) 214
HgaI GACGC 1 cut(s) 47
HhaI GCGC 3 cut(s) 75, 253, 612
Hin1I GRCGYC 2 cut(s) 39, 382
Hin1II CATG 1 cut(s) 442
Hin6I GCGC 3 cut(s) 73, 251, 610
HinP1I GCGC 3 cut(s) 73, 251, 610
HincII GTYRAC 1 cut(s) 55
HindII GTYRAC 1 cut(s) 55
HindIII AAGCTT 1 cut(s) 544
HinfI GANTC 3 cut(s) 217, 668, 688
HpaII CCGG 1 cut(s) 214
HphI GGTGA 1 cut(s) 94
Hpy166II GTNNAC 4 cut(s) 55, 102, 221, 380
Hpy188I TCNGA 2 cut(s) 85, 551
Hpy188III TCNNGA 3 cut(s) 214, 309, 439
Hpy8I GTNNAC 4 cut(s) 55, 102, 221, 380
Hpy99I CGWCG 1 cut(s) 41
HpyCH4III ACNGT 3 cut(s) 316, 496, 587
HpyCH4IV ACGT 2 cut(s) 15, 382
HpyCH4V TGCA 5 cut(s) 147, 284, 344, 538, 602
HpyF10VI GCNNNNNNNGC 4 cut(s) 144, 233, 398, 713
HpyF3I CTNAG 1 cut(s) 254
HpySE526I ACGT 2 cut(s) 15, 382
Hsp92I GRCGYC 2 cut(s) 39, 382
Hsp92II CATG 1 cut(s) 442
HspAI GCGC 3 cut(s) 73, 251, 610
Kpn2I TCCGGA 1 cut(s) 213
KpnI GGTACC 1 cut(s) 291
Kzo9I GATC 3 cut(s) 33, 113, 183
LmnI GCTCC 1 cut(s) 630
Lsp1109I GCAGC 2 cut(s) 328, 700
MaeI CTAG 1 cut(s) 191
MaeII ACGT 2 cut(s) 15, 382
MaeIII GTNAC 1 cut(s) 16
MalI GATC 3 cut(s) 35, 115, 185
MboI GATC 3 cut(s) 33, 113, 183
MboII GAAGA 2 cut(s) 82, 441
MflI RGATCY 1 cut(s) 113
MhlI GDGCHC 2 cut(s) 508, 627
MluCI AATT 4 cut(s) 86, 323, 372, 593
MlyI GAGTC 1 cut(s) 226
MroI TCCGGA 1 cut(s) 213
MseI TTAA 1 cut(s) 491
MspI CCGG 1 cut(s) 214
MspR9I CCNGG 1 cut(s) 97
MvaI CCWGG 1 cut(s) 97
MvnI CGCG 2 cut(s) 48, 73
MwoI GCNNNNNNNGC 4 cut(s) 144, 233, 398, 713
NdeI CATATG 1 cut(s) 410
NdeII GATC 3 cut(s) 33, 113, 183
NlaIII CATG 1 cut(s) 442
NlaIV GGNNCC 1 cut(s) 289
NmuCI GTSAC 1 cut(s) 16
PagI TCATGA 1 cut(s) 438
PcsI WCGNNNNNNNCGW 1 cut(s) 45
PfeI GAWTC 2 cut(s) 668, 688
PflFI GACNNNGTC 1 cut(s) 651
PkrI GCNGC 5 cut(s) 72, 137, 238, 343, 715
PleI GAGTC 1 cut(s) 225
PpsI GAGTC 1 cut(s) 225
Psp124BI GAGCTC 1 cut(s) 627
Psp6I CCWGG 1 cut(s) 95
PspGI CCWGG 1 cut(s) 95
PspN4I GGNNCC 1 cut(s) 289
PspPI GGNCC 1 cut(s) 165
PsuI RGATCY 1 cut(s) 113
PsyI GACNNNGTC 1 cut(s) 651
RsaI GTAC 1 cut(s) 289
RsaNI GTAC 1 cut(s) 288
SacI GAGCTC 1 cut(s) 627
SalI GTCGAC 1 cut(s) 53
SaqAI TTAA 1 cut(s) 491
SatI GCNGC 5 cut(s) 71, 136, 237, 342, 714
Sau3AI GATC 3 cut(s) 33, 113, 183
Sau96I GGNCC 1 cut(s) 165
SchI GAGTC 1 cut(s) 226
ScrFI CCNGG 1 cut(s) 97
SduI GDGCHC 2 cut(s) 508, 627
SfcI CTRYAG 2 cut(s) 160, 616
SinI GGWCC 1 cut(s) 165
SmlI CTYRAG 1 cut(s) 697
SmoI CTYRAG 1 cut(s) 697
Sse9I AATT 4 cut(s) 86, 323, 372, 593
SsiI CCGC 6 cut(s) 23, 46, 68, 71, 136, 236
SspI AATATT 1 cut(s) 405
SspMI CTAG 1 cut(s) 191
SstI GAGCTC 1 cut(s) 627
StyD4I CCNGG 1 cut(s) 95
TaaI ACNGT 3 cut(s) 316, 496, 587
TaiI ACGT 2 cut(s) 18, 385
TaqI TCGA 3 cut(s) 36, 54, 424
TasI AATT 4 cut(s) 86, 323, 372, 593
TauI GCSGC 3 cut(s) 73, 138, 239
TfiI GAWTC 2 cut(s) 668, 688
Tru1I TTAA 1 cut(s) 491
Tru9I TTAA 1 cut(s) 491
TseFI GTSAC 1 cut(s) 16
TseI GCWGC 2 cut(s) 341, 713
Tsp45I GTSAC 1 cut(s) 16
TspDTI ATGAA 2 cut(s) 397, 451
Tth111I GACNNNGTC 1 cut(s) 651
VpaK11BI GGWCC 1 cut(s) 165
XagI CCTNNNNNAGG 1 cut(s) 47
XapI RAATTY 2 cut(s) 86, 593
XmiI GTMKAC 1 cut(s) 54
XspI CTAG 1 cut(s) 191
ZraI GACGTC 1 cut(s) 383
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.