Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr04

Orthogroup ID

OG0008676

Physical Location & Seq

Genomic Coordinates Forward (+)

23065417 .. 23069853

Gene Structure 4437 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

MD04G1141700.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 2832 bp

ATGGGATTCAGTTTAGAGCAACGAAATAGTTCAAAAGAGCAGAACAGGTCCAGCATAGAGGACAATGTTGTACAGCCCCAAACAAGTCGTAGAACAAGGCATATAGAGAAGGCGAAAGTGAGAACTGGTGCTGGTTTGAAGCAGAATGATCTGCATCAGAATGCAAGACAAGATGCAGGTGATGGTGCTTCAGGCCAAGAAAAATTTTCGGGAAATCGATCTAGAGAGAGTATGAAGGGGAATAATGCAGGTAAAGTTGATGAGCTTGTCAAGCACATGTCAAACTTGCCTGGTTATCTTCAGCGCCCTGAGAGAGGAGAAAAGATCCAAGAAAAGGTTTTGAATGTTGGAGTTCTGGATTGGGGTCGACTAGAGAAATGGAAGCACAAACAGAAGCCTTTTCCCGAAAAAGGTAGCGGTAGTTCATCCTCAGAAAGGACTACTGGAAAGTCTACATCATCTGTTGTAGTTCACAATGGGACAAATGCTGAAGAACTCTCTTCAGGTTGTTCTAGTCTCAAGTCGTCTCAAAAGGATGGCCTTTCGCAGGGTGTCAAACCATCTGTGCATAAAGATGTGCGCTTCCAAGACTCTAAAACTGCTTCGAAGAATGCCATTTATGGGCAGAAAAAGATATCCATTCCCTATCGGTCCCTTGGAAGACATCACTCAGATATGATGCTCGATAAGGGGAAGGCAAAAGATTCAGATCAGAAGTTCACATCAGAAACGGGCAATATGGCAGCAAACTTGAAAAGCTATGGAGTCTCTCTCAATCAAAAGCACGAGATGAGCACTAGGCATGGTCGAGCTAAAAAAACCAAGGACCTGCAAGAATCGGATATCAAGAGGAAGGATATTGATCAAGGAATCATTACGGAAAAGGGAGTTCCTTCATCTAAGCTCGAAGGTTTTGATGTCTCACTCAGTTCAGAGGGAAAGACAATTGCTTGTCATGGTAAAACTGAGAAAAGGGTGGAGGAGCTGCACAAATCAGTTACCAATCTAGCTCATCAACACTGCCCTTCTGCAGAGAATCCCGTTGTTGTCTCACCACCAAAGGAACTACCCCAAAATGACTTTCCAGAGGTACTCCAGCTTTCAAAACCAAGAGCATCATGGGATGAGTGTATGGCAGAAGCTGATAAAATTAGCTTCTCGGGCAACTTTTCGACCAAAGAGATGGACTTTGCCGAATTCTCTTCTGAAGTTTCACACTCATGCCCACTGTCTTCTGCAGTTGAGACTAATACAGCATCAAACATGTTGCTAAATAGCACAATCAACGGTAACAGTGTGGGGCTTTCCTTTGTTCCATCTCACATGCGTCGATGCTCAACACAAGACCTCCTCTATGATGATAAATTTGTACATAAGAAAAATTCAGAGAAAATGCTTACCCGTTCAAGCTTTGATACTTCGAGTACATTGGATCAAGAAGATGTTGAACTGGCAACTAGAAAAGGCAGAAGCCCAACTCCCATTCGCCGGTTTAACTTCAGCTTAGGCCGGCTTGGTAGAAGTCTCAGTTTTAAGGAAATTTCAGATATTCCACAGTTGAGTTCCACATATACGACGGTCAAATCAGGTCCTGTGAGATCTGGAACTTCTGATTTTCCAGATAATCCAAACAGAGAGAAAGCAAGTACTCATAACCGAGCTAGGTCAAGCCCGTTAAGAAGGCTACTAGACCCAATATTAAAGCACAAGGAGGCAAATCTACACCATTCTGCTGAAGCTGTTAGACCCCCGAAATCAAATTTTAATTCCTTGGTCCCCAAGCCAATTAATATCAGCGAGTCATTAGAGAATCTGAAGGCTGAGGCATCATGGGTTAAAGCTTTTTTGCAAATAACAATCAAGAATGGACTTCCTTTGTTTAAGTTTTGGGTTGACAACAACAGCAACTGTTTTGCGGCAACCATGAAGAATTTATCATCCGGGAAGGATGATTTCTGCCAGTATTTTACATTTTACTGTATTAATGAAGTTAAGAGAAAAAGTGGTGGCTGGATGAGTCAAGGAAGTAAAGGCAAAAGTTGTCACTATGCTTACAACATTGTTGCTCAAATGAAAGTTTCTAGCTCTGACTTATCTGATGTTAACGGGCAGGACTTGAGCAAGTATATGGTAAGAGAGGCTGTTTTGTTAGATGTTGATCTAACACAAGCAGATCAAGAATCACCTAGCGTTGTACCACATAGGGAGCTTGCTGCTGCTGTGGTCAAGTTACCTAGGAAAGACTTGAGCCATCCTGAACAACAGAGTGATGAGGAAGTTATGGAGAAGGGCTGCGCGAAAGGTTCATCCGAGGATTACAGCTGGGAAGATAGTACTATAGTCATACTTCCAGGTGGAGTCCATAGCTCACCGAACACAGGAGAACCTTCACCATTACTTGACCGTTGGAAATCTGGTGGGTTATGTGATTGTGGAGGCTGGGATGTTGGTTGTAAGCTGCGTGTTCTCTCCAACCAGAACAAATGCTGTCGGATTCCAAAAACATCCTCCGCATGTTACCCTTTTCCAGAGACTTTTGAACTATTTGCGGAGGAGGGAGCTCAGCAGAACAGGCCAATCTTCAGTTTGGCACCAGGGAAGGATGGAAGCTACTCGATTGAATTCAACACGTCACTTTCTTTATTACAAGCGTTCTTCATCTGTGTTGTCGTAATCAGCAGTTGCAGACCATCAGATCTTTCAGAAGTTAGTAACGTGTTGGAGGCGAAAGAATTCCAGGAACCGAATCTGAATCGAAGTAATGGAATCCAGGTGACAGGTCCGGCAAAGTATGCTCCAAATCCACCCCTGTCACCTGTTGGGAGGGTCTAG

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

No ontology terms assigned.

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

DUF3527

Protein Analysis

944

Amino Acids

104.07

Weight (kDa)

8.84

Isoelectric Point (pI)

52.58

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
DUF3527	PF12043	612 - 726	3.7e-10	Domain of unknown function (DUF3527)
DUF3527	PF12043	729 - 892	1.9e-37	Domain of unknown function (DUF3527)

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0008676)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT5G01030 AT5G01030 AT5G01030
fragaria_vesca	FvH4_6g12500 FvH4_6g12500
malus_domestica	MD04G1141700.v1.1 MD12G1156300.v1.1
prunus_persica	Prupe.6G267100_v2.0.a1
pyrus_communis	pycom04g12880 pycom12g14840
rosa_chinensis	RchiOBHm_Chr3g0464321
rosa_laevigata	RLG00000024708
rosa_multiflora	Rmu_sc0003074.1_g000030 Rmu_sc0007811.1_g000001 Rmu_sc0029720.1_g000001
rosa_roxburghii	Rroxscaffold_6G00415810
rosa_rugosa	Rorug03G0071300 Rorug03G0071300 Rorug03G0071400
rosa_samantha	Rh3BG133500 Rh3CG135800 Rh3DG135000
rosa_wichuraiana	Rw3G010940

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AarI	CACCTGC	1 cut(s)	167
Acc36I	ACCTGC	3 cut(s)	167, 239, 837
AccB1I	GGYRCC	1 cut(s)	2590
AccI	GTMKAC	2 cut(s)	367, 452
AccII	CGCG	1 cut(s)	2297
AciI	CCGC	4 cut(s)	417, 1916, 2511, 2549
AclWI	GGATC	2 cut(s)	319, 1440
AcsI	RAATTY	9 cut(s)	203, 1196, 1364, 1381, 1539, 1759, 1930, 2621, 2732
AcuI	CTGAAG	9 cut(s)	174, 284, 486, 510, 1227, 1483, 1755, 1835, 2566
AfaI	GTAC	7 cut(s)	72, 1092, 1371, 1426, 1648, 2196, 2335
AfiI	CCNNNNNNNGG	8 cut(s)	314, 334, 410, 435, 547, 621, 1488, 2378
AflIII	ACRYGT	4 cut(s)	276, 1263, 2628, 2715
AjiI	CACGTC	1 cut(s)	2631
AjnI	CCWGG	5 cut(s)	289, 2350, 2593, 2736, 2769
AjuI	GAANNNNNNNTTGG	2 cut(s)	1065, 1097
Alw21I	GWGCWC	2 cut(s)	797, 2563
Alw26I	GTCTC	8 cut(s)	521, 531, 772, 925, 1054, 1238, 1529, 2525
AlwI	GGATC	2 cut(s)	319, 1440
AlwNI	CAGNNNCTG	3 cut(s)	1142, 1592, 1908
Ama87I	CYCGRG	1 cut(s)	1159
AoxI	GGCC	4 cut(s)	193, 538, 1507, 2573
ApeKI	GCWGC	6 cut(s)	743, 985, 2213, 2216, 2292, 2458
ApoI	RAATTY	9 cut(s)	203, 1196, 1364, 1381, 1539, 1759, 1930, 2621, 2732
AseI	ATTAAT	2 cut(s)	1788, 1983
Asp700I	GAANNNNTTC	2 cut(s)	28, 2732
AspA2I	CCTAGG	1 cut(s)	2234
AspLEI	GCGC	3 cut(s)	306, 582, 2297
AspS9I	GGNCC	6 cut(s)	48, 651, 826, 1589, 1774, 2780
AsuC2I	CCSGG	1 cut(s)	1942
AsuHPI	GGTGA	7 cut(s)	191, 1044, 2175, 2361, 2382, 2785, 2805
AsuII	TTCGAA	1 cut(s)	605
AvaI	CYCGRG	1 cut(s)	1159
AvaII	GGWCC	6 cut(s)	48, 651, 826, 1589, 1774, 2780
AvrII	CCTAGG	1 cut(s)	2234
BanI	GGYRCC	1 cut(s)	2590
BanII	GRGCYC	1 cut(s)	2563
BauI	CACGAG	1 cut(s)	785
BbsI	GAAGAC	2 cut(s)	667, 1224
Bbv12I	GWGCWC	2 cut(s)	797, 2563
BbvCI	CCTCAGC	1 cut(s)	1821
BbvI	GCAGC	6 cut(s)	755, 972, 2200, 2203, 2279, 2445
BccI	CCATC	8 cut(s)	176, 530, 568, 1177, 1324, 2259, 2597, 2698
BciT130I	CCWGG	5 cut(s)	291, 2352, 2595, 2738, 2771
BclI	TGATCA	1 cut(s)	862
BcnI	CCSGG	1 cut(s)	1942
BcoDI	GTCTC	8 cut(s)	521, 531, 772, 925, 1054, 1238, 1529, 2525
BfmI	CTRYAG	3 cut(s)	1029, 1236, 2337
BfoI	RGCGCY	1 cut(s)	307
BfuAI	ACCTGC	3 cut(s)	167, 239, 837
BglII	AGATCT	2 cut(s)	1598, 2695
BisI	GCNGC	7 cut(s)	744, 986, 1917, 2214, 2217, 2293, 2459
BlnI	CCTAGG	1 cut(s)	2234
BlpI	GCTNAGC	1 cut(s)	2562
BlsI	GCNGC	7 cut(s)	745, 987, 1918, 2215, 2218, 2294, 2460
BmcAI	AGTACT	2 cut(s)	1648, 2335
Bme1390I	CCNGG	6 cut(s)	291, 1942, 2352, 2595, 2738, 2771
Bme18I	GGWCC	6 cut(s)	48, 651, 826, 1589, 1774, 2780
BmeT110I	CYCGRG	1 cut(s)	1159
BmgBI	CACGTC	1 cut(s)	2631
BmgT120I	GGNCC	6 cut(s)	48, 651, 826, 1589, 1774, 2780
BmiI	GGNNCC	4 cut(s)	653, 1776, 2592, 2742
BmrFI	CCNGG	6 cut(s)	291, 1942, 2352, 2595, 2738, 2771
BmsI	GCATC	7 cut(s)	163, 163, 669, 1124, 1265, 1322, 1835
BpiI	GAAGAC	2 cut(s)	667, 1224
BplI	GAGNNNNNCTC	2 cut(s)	756, 788
BpmI	CTGGAG	1 cut(s)	1079
Bpu10I	CCTNAGC	2 cut(s)	1504, 1821
Bpu1102I	GCTNAGC	1 cut(s)	2562
Bpu14I	TTCGAA	1 cut(s)	605
BpuEI	CTTGAG	3 cut(s)	503, 2137, 2266
BpuMI	CCSGG	1 cut(s)	1942
Bsa29I	ATCGAT	1 cut(s)	217
BsaBI	GATNNNNATC	4 cut(s)	153, 708, 861, 2157
BsaJI	CCNNGG	6 cut(s)	655, 822, 1770, 2234, 2310, 2594
BsaXI	ACNNNNNCTCC	2 cut(s)	1332, 1362
Bsc4I	CCNNNNNNNGG	8 cut(s)	314, 334, 410, 435, 547, 621, 1488, 2378
Bse118I	RCCGGY	2 cut(s)	1488, 1509
Bse1I	ACTGG	4 cut(s)	130, 448, 1455, 1960
Bse8I	GATNNNNATC	4 cut(s)	153, 708, 861, 2157
BseBI	CCWGG	5 cut(s)	291, 2352, 2595, 2738, 2771
BseCI	ATCGAT	1 cut(s)	217
BseDI	CCNNGG	6 cut(s)	655, 822, 1770, 2234, 2310, 2594
BseJI	GATNNNNATC	4 cut(s)	153, 708, 861, 2157
BseLI	CCNNNNNNNGG	8 cut(s)	314, 334, 410, 435, 547, 621, 1488, 2378
BseMII	CTCAG	8 cut(s)	300, 444, 684, 940, 957, 1540, 1812, 2576
BseNI	ACTGG	4 cut(s)	130, 448, 1455, 1960
BseRI	GAGGAG	4 cut(s)	330, 995, 1340, 2567
BseXI	GCAGC	6 cut(s)	755, 972, 2200, 2203, 2279, 2445
BseYI	CCCAGC	2 cut(s)	2322, 2439
BsgI	GTGCAG	1 cut(s)	971
Bsh1236I	CGCG	1 cut(s)	2297
BshFI	GGCC	4 cut(s)	195, 540, 1509, 2575
BshNI	GGYRCC	1 cut(s)	2590
BshVI	ATCGAT	1 cut(s)	217
BsiHKAI	GWGCWC	2 cut(s)	797, 2563
BsiHKCI	CYCGRG	1 cut(s)	1159
BsiSI	CCGG	4 cut(s)	1489, 1510, 1941, 2783
BslFI	GGGAC	3 cut(s)	493, 637, 1760
BslI	CCNNNNNNNGG	8 cut(s)	314, 334, 410, 435, 547, 621, 1488, 2378
BsmAI	GTCTC	8 cut(s)	521, 531, 772, 925, 1054, 1238, 1529, 2525
BsmBI	CGTCTC	1 cut(s)	531
BsmFI	GGGAC	3 cut(s)	493, 637, 1760
BsmI	GAATGC	2 cut(s)	166, 616
BsnI	GGCC	4 cut(s)	195, 540, 1509, 2575
BsoBI	CYCGRG	1 cut(s)	1159
Bsp119I	TTCGAA	1 cut(s)	605
Bsp1286I	GDGCHC	2 cut(s)	797, 2563
Bsp1407I	TGTACA	2 cut(s)	70, 1369
Bsp1720I	GCTNAGC	1 cut(s)	2562
BspACI	CCGC	4 cut(s)	417, 1916, 2511, 2549
BspANI	GGCC	4 cut(s)	195, 540, 1509, 2575
BspCNI	CTCAG	8 cut(s)	301, 443, 683, 939, 958, 1539, 1813, 2575
BspDI	ATCGAT	1 cut(s)	217
BspFNI	CGCG	1 cut(s)	2297
BspLI	GGNNCC	4 cut(s)	653, 1776, 2592, 2742
BspMAI	CTGCAG	2 cut(s)	1033, 1240
BspMI	ACCTGC	3 cut(s)	167, 239, 837
BspPI	GGATC	2 cut(s)	319, 1440
BspT104I	TTCGAA	1 cut(s)	605
BspT107I	GGYRCC	1 cut(s)	2590
BsrFI	RCCGGY	2 cut(s)	1488, 1509
BsrGI	TGTACA	2 cut(s)	70, 1369
BsrI	ACTGG	4 cut(s)	130, 448, 1455, 1960
BssAI	RCCGGY	2 cut(s)	1488, 1509
BssECI	CCNNGG	6 cut(s)	655, 822, 1770, 2234, 2310, 2594
BssSI	CACGAG	1 cut(s)	785
BssT1I	CCWWGG	4 cut(s)	655, 822, 1770, 2234
Bst2BI	CACGAG	1 cut(s)	785
Bst2UI	CCWGG	5 cut(s)	291, 2352, 2595, 2738, 2771
Bst4CI	ACNGT	8 cut(s)	1230, 1289, 1295, 1557, 1579, 1910, 1979, 2405
Bst6I	CTCTTC	2 cut(s)	505, 1207
BstAPI	GCANNNNNTGC	1 cut(s)	2792
BstAUI	TGTACA	2 cut(s)	70, 1369
BstBI	TTCGAA	1 cut(s)	605
BstC8I	GCNNGC	2 cut(s)	1511, 2211
BstENI	CCTNNNNNAGG	3 cut(s)	312, 433, 545
BstFNI	CGCG	1 cut(s)	2297
BstH2I	RGCGCY	1 cut(s)	307
BstHHI	GCGC	3 cut(s)	306, 582, 2297
BstMAI	GTCTC	8 cut(s)	521, 531, 772, 925, 1054, 1238, 1529, 2525
BstMWI	GCNNNNNNNGC	4 cut(s)	271, 1161, 2572, 2792
BstNI	CCWGG	5 cut(s)	291, 2352, 2595, 2738, 2771
BstNSI	RCATGY	4 cut(s)	280, 1267, 1327, 2517
BstSCI	CCNGG	6 cut(s)	289, 1940, 2350, 2593, 2736, 2769
BstSFI	CTRYAG	3 cut(s)	1029, 1236, 2337
BstUI	CGCG	1 cut(s)	2297
BstV1I	GCAGC	6 cut(s)	755, 972, 2200, 2203, 2279, 2445
BstV2I	GAAGAC	2 cut(s)	667, 1224
BstX2I	RGATCY	3 cut(s)	324, 1598, 2695
BstXI	CCANNNNNNTGG	1 cut(s)	1183
BstYI	RGATCY	3 cut(s)	324, 1598, 2695
Bsu15I	ATCGAT	1 cut(s)	217
BsuRI	GGCC	4 cut(s)	195, 540, 1509, 2575
BsuTUI	ATCGAT	1 cut(s)	217
BtrI	CACGTC	1 cut(s)	2631
BtsI	GCAGTG	1 cut(s)	1018
BtsIMutI	CAGTG	3 cut(s)	1018, 1226, 1300
BveI	ACCTGC	3 cut(s)	167, 239, 837
Cac8I	GCNNGC	2 cut(s)	1511, 2211
CaiI	CAGNNNCTG	3 cut(s)	1142, 1592, 1908
CfoI	GCGC	3 cut(s)	306, 582, 2297
Cfr10I	RCCGGY	2 cut(s)	1488, 1509
Cfr13I	GGNCC	6 cut(s)	48, 651, 826, 1589, 1774, 2780
ClaI	ATCGAT	1 cut(s)	217
CseI	GACGC	1 cut(s)	1316
Csp6I	GTAC	7 cut(s)	71, 1091, 1370, 1425, 1647, 2195, 2334
CviQI	GTAC	7 cut(s)	71, 1091, 1370, 1425, 1647, 2195, 2334
Eam1104I	CTCTTC	2 cut(s)	505, 1207
EarI	CTCTTC	2 cut(s)	505, 1207
Ecl136II	GAGCTC	1 cut(s)	2561
Eco130I	CCWWGG	4 cut(s)	655, 822, 1770, 2234
Eco24I	GRGCYC	1 cut(s)	2563
Eco32I	GATATC	2 cut(s)	636, 844
Eco47I	GGWCC	6 cut(s)	48, 651, 826, 1589, 1774, 2780
Eco53kI	GAGCTC	1 cut(s)	2561
Eco57I	CTGAAG	9 cut(s)	174, 284, 486, 510, 1227, 1483, 1755, 1835, 2566
Eco88I	CYCGRG	1 cut(s)	1159
EcoICRI	GAGCTC	1 cut(s)	2561
EcoNI	CCTNNNNNAGG	3 cut(s)	312, 433, 545
EcoO109I	RGGNCCY	2 cut(s)	826, 1589
EcoRI	GAATTC	3 cut(s)	1196, 2621, 2732
EcoRII	CCWGG	5 cut(s)	289, 2350, 2593, 2736, 2769
EcoRV	GATATC	2 cut(s)	636, 844
EcoT14I	CCWWGG	4 cut(s)	655, 822, 1770, 2234
EcoT38I	GRGCYC	1 cut(s)	2563
ErhI	CCWWGG	4 cut(s)	655, 822, 1770, 2234
Esp3I	CGTCTC	1 cut(s)	531
FaqI	GGGAC	3 cut(s)	493, 637, 1760
FbaI	TGATCA	1 cut(s)	862
FblI	GTMKAC	2 cut(s)	367, 452
Fnu4HI	GCNGC	7 cut(s)	744, 986, 1917, 2214, 2217, 2293, 2459
FriOI	GRGCYC	1 cut(s)	2563
Fsp4HI	GCNGC	7 cut(s)	744, 986, 1917, 2214, 2217, 2293, 2459
GlaI	GCGC	3 cut(s)	305, 581, 2296
GluI	GCNGC	7 cut(s)	744, 986, 1917, 2214, 2217, 2293, 2459
GsaI	CCCAGC	2 cut(s)	2326, 2443
GsuI	CTGGAG	1 cut(s)	1079
HaeII	RGCGCY	1 cut(s)	307
HaeIII	GGCC	4 cut(s)	195, 540, 1509, 2575
HapII	CCGG	4 cut(s)	1489, 1510, 1941, 2783
HgaI	GACGC	1 cut(s)	1316
HhaI	GCGC	3 cut(s)	306, 582, 2297
Hin6I	GCGC	3 cut(s)	304, 580, 2295
HinP1I	GCGC	3 cut(s)	304, 580, 2295
HincII	GTYRAC	3 cut(s)	368, 1894, 2104
HindII	GTYRAC	3 cut(s)	368, 1894, 2104
HindIII	AAGCTT	2 cut(s)	1408, 1839
HpaI	GTTAAC	1 cut(s)	2104
HpaII	CCGG	4 cut(s)	1489, 1510, 1941, 2783
HphI	GGTGA	7 cut(s)	191, 1044, 2175, 2361, 2382, 2785, 2805
Hpy166II	GTNNAC	6 cut(s)	368, 453, 472, 720, 1894, 2104
Hpy8I	GTNNAC	6 cut(s)	368, 453, 472, 720, 1894, 2104
Hpy99I	CGWCG	2 cut(s)	1332, 1579
HpyCH4III	ACNGT	8 cut(s)	1230, 1289, 1295, 1557, 1579, 1910, 1979, 2405
HpyCH4IV	ACGT	2 cut(s)	2630, 2715
HpyF10VI	GCNNNNNNNGC	4 cut(s)	271, 1161, 2572, 2792
HpySE526I	ACGT	2 cut(s)	2630, 2715
HspAI	GCGC	3 cut(s)	304, 580, 2295
KroI	GCCGGC	1 cut(s)	1509
KroNI	GCCGGC	1 cut(s)	1511
Ksp22I	TGATCA	1 cut(s)	862
KspAI	GTTAAC	1 cut(s)	2104
LmnI	GCTCC	4 cut(s)	982, 2206, 2558, 2800
Lsp1109I	GCAGC	6 cut(s)	755, 972, 2200, 2203, 2279, 2445
LweI	GCATC	7 cut(s)	163, 163, 669, 1124, 1265, 1322, 1835
MaeII	ACGT	2 cut(s)	2630, 2715
MaeIII	GTNAC	9 cut(s)	997, 1289, 2042, 2229, 2516, 2631, 2711, 2773, 2811
MfeI	CAATTG	1 cut(s)	945
MflI	RGATCY	3 cut(s)	324, 1598, 2695
MhlI	GDGCHC	2 cut(s)	797, 2563
MlyI	GAGTC	5 cut(s)	584, 774, 1808, 2026, 2367
MmeI	TCCRAC	5 cut(s)	328, 2387, 2471, 2496, 2700
MroNI	GCCGGC	1 cut(s)	1509
MroXI	GAANNNNTTC	2 cut(s)	28, 2732
MslI	CAYNNNNRTG	3 cut(s)	159, 573, 1219
MspA1I	CMGCKG	1 cut(s)	2322
MspI	CCGG	4 cut(s)	1489, 1510, 1941, 2783
MspR9I	CCNGG	6 cut(s)	291, 1942, 2352, 2595, 2738, 2771
MunI	CAATTG	1 cut(s)	945
Mva1269I	GAATGC	2 cut(s)	166, 616
MvaI	CCWGG	5 cut(s)	291, 2352, 2595, 2738, 2771
MvnI	CGCG	1 cut(s)	2297
MwoI	GCNNNNNNNGC	4 cut(s)	271, 1161, 2572, 2792
NaeI	GCCGGC	1 cut(s)	1511
NciI	CCSGG	1 cut(s)	1942
NgoMIV	GCCGGC	1 cut(s)	1509
NlaIV	GGNNCC	4 cut(s)	653, 1776, 2592, 2742
NmuCI	GTSAC	4 cut(s)	2042, 2631, 2773, 2811
NspI	RCATGY	4 cut(s)	280, 1267, 1327, 2517
NspV	TTCGAA	1 cut(s)	605
PaqCI	CACCTGC	1 cut(s)	167
PciI	ACATGT	2 cut(s)	276, 1263
PctI	GAATGC	2 cut(s)	166, 616
PdiI	GCCGGC	1 cut(s)	1511
PdmI	GAANNNNTTC	2 cut(s)	28, 2732
PfoI	TCCNGGA	2 cut(s)	1940, 2736
PkrI	GCNGC	7 cut(s)	745, 987, 1918, 2215, 2218, 2294, 2460
PleI	GAGTC	5 cut(s)	584, 773, 1807, 2025, 2366
PpsI	GAGTC	5 cut(s)	584, 773, 1807, 2025, 2366
PpuMI	RGGWCCY	2 cut(s)	826, 1589
PscI	ACATGT	2 cut(s)	276, 1263
PshBI	ATTAAT	2 cut(s)	1788, 1983
Psp124BI	GAGCTC	1 cut(s)	2563
Psp5II	RGGWCCY	2 cut(s)	826, 1589
Psp6I	CCWGG	5 cut(s)	289, 2350, 2593, 2736, 2769
PspFI	CCCAGC	2 cut(s)	2322, 2439
PspGI	CCWGG	5 cut(s)	289, 2350, 2593, 2736, 2769
PspN4I	GGNNCC	4 cut(s)	653, 1776, 2592, 2742
PspPI	GGNCC	6 cut(s)	48, 651, 826, 1589, 1774, 2780
PspPPI	RGGWCCY	2 cut(s)	826, 1589
PsrI	GAACNNNNNNTAC	2 cut(s)	406, 438
PstI	CTGCAG	2 cut(s)	1033, 1240
PstNI	CAGNNNCTG	3 cut(s)	1142, 1592, 1908
PsuI	RGATCY	3 cut(s)	324, 1598, 2695
PvuII	CAGCTG	1 cut(s)	2322
RsaI	GTAC	7 cut(s)	72, 1092, 1371, 1426, 1648, 2196, 2335
RsaNI	GTAC	7 cut(s)	71, 1091, 1370, 1425, 1647, 2195, 2334
RseI	CAYNNNNRTG	3 cut(s)	159, 573, 1219
SacI	GAGCTC	1 cut(s)	2563
SalI	GTCGAC	1 cut(s)	366
SatI	GCNGC	7 cut(s)	744, 986, 1917, 2214, 2217, 2293, 2459
Sau96I	GGNCC	6 cut(s)	48, 651, 826, 1589, 1774, 2780
ScaI	AGTACT	2 cut(s)	1648, 2335
SchI	GAGTC	5 cut(s)	584, 774, 1808, 2026, 2367
ScrFI	CCNGG	6 cut(s)	291, 1942, 2352, 2595, 2738, 2771
SduI	GDGCHC	2 cut(s)	797, 2563
SfaNI	GCATC	7 cut(s)	163, 163, 669, 1124, 1265, 1322, 1835
SfcI	CTRYAG	3 cut(s)	1029, 1236, 2337
SfuI	TTCGAA	1 cut(s)	605
SinI	GGWCC	6 cut(s)	48, 651, 826, 1589, 1774, 2780
SmiMI	CAYNNNNRTG	3 cut(s)	159, 573, 1219
SmlI	CTYRAG	3 cut(s)	518, 2116, 2245
SmoI	CTYRAG	3 cut(s)	518, 2116, 2245
SsiI	CCGC	4 cut(s)	417, 1916, 2511, 2549
SspI	AATATT	1 cut(s)	1698
SstI	GAGCTC	1 cut(s)	2563
StyD4I	CCNGG	6 cut(s)	289, 1940, 2350, 2593, 2736, 2769
StyI	CCWWGG	4 cut(s)	655, 822, 1770, 2234
TaaI	ACNGT	8 cut(s)	1230, 1289, 1295, 1557, 1579, 1910, 1979, 2405
TaiI	ACGT	2 cut(s)	2633, 2718
TaqII	GACCGA	1 cut(s)	639
TatI	WGTACW	5 cut(s)	70, 1369, 1424, 1646, 2333
TauI	GCSGC	1 cut(s)	1919
TscAI	CASTG	3 cut(s)	1025, 1233, 1300
TseFI	GTSAC	4 cut(s)	2042, 2631, 2773, 2811
TseI	GCWGC	6 cut(s)	743, 985, 2213, 2216, 2292, 2458
Tsp45I	GTSAC	4 cut(s)	2042, 2631, 2773, 2811
TspDTI	ATGAA	8 cut(s)	248, 414, 885, 1940, 2001, 2087, 2295, 2647
TspGWI	ACGGA	1 cut(s)	893
TspRI	CASTG	3 cut(s)	1025, 1233, 1300
VpaK11BI	GGWCC	6 cut(s)	48, 651, 826, 1589, 1774, 2780
VspI	ATTAAT	2 cut(s)	1788, 1983
XagI	CCTNNNNNAGG	3 cut(s)	312, 433, 545
XapI	RAATTY	9 cut(s)	203, 1196, 1364, 1381, 1539, 1759, 1930, 2621, 2732
XbaI	TCTAGA	1 cut(s)	221
XceI	RCATGY	4 cut(s)	280, 1267, 1327, 2517
XcmI	CCANNNNNNNNNTGG	1 cut(s)	1116
XmaJI	CCTAGG	1 cut(s)	2234
XmiI	GTMKAC	2 cut(s)	367, 452
XmnI	GAANNNNTTC	2 cut(s)	28, 2732
ZrmI	AGTACT	2 cut(s)	1648, 2335

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.