Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr12

Orthogroup ID

OG0008676

Physical Location & Seq

Genomic Coordinates Forward (+)

23695251 .. 23699229

Gene Structure 3979 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

MD12G1156300.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 2796 bp

ATGGGAAGTACCTCAAAAGTGCAAGACAATGTTGTGCAGCGCCAAACTAGTCGAAGAACAAGACATGTAGAGAAGGCGAAGGTGAGAACTGGTGTTGGTTTGCAGCAGAATGATGTGCATTGTAAAGCAAGAAAAGATGCAGGTGATGGTGCTGTGGACCAAGAAAAGTCTTCGGGAAGTCAAAATGGGAAGGGGAATGATGCAGCTAAAGTTGATGAGCTGGTCAAGCACATGTCAAACTTGCCCGGTTATCTTCAACGCCCTGCGAGAGGAGAAAAAAACCAAGAGAAGGTTTTGAATGTTGGGGTTTTGGATTGGGGTCGACTAGAGAAATGGAAGCACAAGCAGAAGCCTTTTCCTGAAAAAGTTAGCGGCAGGTCATCCTTGGAAAGGGCTATTGGATCATCTACATCATTTGTTGTCGTTCGCAATGGGACAAATGCTGAGCAACCCTCTTCGCGTTCTAGTGTCAAGTCGTCTCAAAAGAATGGCCTTTCACAGGGTGTCAAACCATCTGTCCGTAAAGATATGCGCTTCCAGGACTCCAAAACTGCTTCAAAGAATGCCATTCATGGGCAGAAAAAGATATCCTTTCCCAGGTCTCTTGGAAGAAATCACTCAGATATGATGCTTGATAAGGGAAAGGGAAAATATTCAGATAAGAAGTTCCCTTCAGAAATGGGGAATGCAGCGGCAAACTTGAAAAAGTATGGAGTGTCATTTGGTCCAAAGGACAAGGTTGGCACAGGGGATGGTCGAGCTATGGAAACAGAATCAGATATCAAGAAGAAAAATAATGATCAAAGAATCACTACGGAAAAGGGAGCTCCATCGCCTAAATTCAGAAGTTATGATGCCTCGCTGAGTTCAAAGGGAAAGACAATTGCTTGTAATGATAAAACTGAGAATAGGGTGGAAGAATTGCACAAATCAAACATCAGCACAGCTCATCAACACTGCCCTGCTGCGGAGAATACCGTTGTTCTTTCTCCATCAAAGGAACTGCCCCAAAATGACTTTCCAGAGGTCCTCCAGCTTTCACAACCAAGAGCATCAGGGGATGAGTGTATGACAGAACCTGATAAGATCAGCTTCTCGGGAGACTTTTCTACCAAGGAGATGGACTTTGCAGAACTCTGTTCAGAAGTTCCTTCTGAAGTTCCACACTCATGCCCCTTGCCTTCTGTAGTTGACACTAATACAGGATCAAACATGGTACCGAATAGCTCAATCAACGGTAAGAGTGCAGGGCTTAACTTTGTCCCAGCTCACATGCGCCAATGCTCAAACGAAACACAAGACCTCCTATATGAAGATAAATTTGTACAGAAGAACAGTTCAGAGAAAATGCTTACTCGTTCAAGTTTTGATACTTCAAAAACATTGGATCAAGAAGATGTTGAGCTAGCAACTAGAAAAGGCAGAAGCCCAACCCCCCATCGCCGGTTTAACTTCAGCTTAGAACGGCTTGGTAGAAGTTTAAGTTTTAAGGAAAGTTCAGATATTCCGCAGTTAAGTTCCACATATATGACAGTCAAATCAGGTCCTGTGAGATCCGGAACTTCTGACTGTCCAGATAATCCAAACAGAGAGAAAGCAAGTACTCATAACCGAGCTAGGTCAAGCCCGTTAAGAAGGTTACTAGACCCAATATTAAAGCACAAGGAGGCAAATCTACACCATTCTGCTGAAGCTGTTAGACCCCCGAAATCACTTTTTAATTCCTTCGTCCCCAAGCCAATTAATATCAGCGAGTCATTACAGAATCTGAAGGCTGAGGCATCATGGGTTAAAGCATCTTTGCAAATAACAATCAAGAATGGACTTCCTTTGTTTAAGTTTTGGGTTGACAACATCAACTGTTTTGCGGCAACCATGAAGAATTTATCATCTGGGAAGGATGACTTCTGCCAGTATTTTACATTTTACTGTATTAATGAAGCCAAGAAAAAAATTGGTGGCTGGATGAGTCAAGGAAGTAAAGGCAAAAGTTGTCGCTATGCCTACAATGTTGTTGCTCAAATGAAAGTTTCTAGCTCCGACTTATCTGATGTTAACAGGCAGGACTTAAGCAAGTGTGTGGTAAGAGAGGCTGTTATGTTAGGTGTTGATCTAACACAAGCAGATCTAGAATCACCAAGCGTCGTACCACATGTGGAGCTTGCTGCTGCTGTTGTCAAGTTACCTAGGAAAGACTTGAGCCATTCTGAACAGCAGTGTGACGAGGAAGTTATGGAGAAGAAGGGCTGCGCCAAAGATTCATCCGAGGATATCTGCTCTTACAGCTGGGAAGATAGTACTATAGTCATACTTCCAGGTGGAGTCCATAGCTCACCGAACAAAGGAGCACCTTCACCATTACTTGACCGTTGGAAATCTGGTGGGTTATGTGATTGTGGAGGCTGGGATGTTGGTTGTAAGCTGCATGTTCTCTCAAACCAGAACAAATGCTGTCAGAGTCCAAAAACATCTTCCGCATGTTACCCTTTTCCGGATACTTTTGAACTATTTGCTGAGGAGGGAGCTCAGGAGAACAGGCCAATTTTCAGTTTGGCACCAGGGAAGGATGGAAGCTACTCGGTTGAATTCAACACGTCACTTTCTTTGTTACAAGCGTTCTTCATCTGTGTTGTCATAATCAGCAGTTGCAAACCATCAGATCTTTCAGAAGTTAGTAACATGACAGAAGCGAAAGATTTCCAGGAACCGAATTTGAATCAAAGTAATGGAATCCAGGTGACAGGGCCGGCAAAGTATGCTCCAAATCCACCCATGTCCCCTGTTGGGAGGGTCTAG

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

No ontology terms assigned.

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

DUF3527

Protein Analysis

932

Amino Acids

102.21

Weight (kDa)

8.94

Isoelectric Point (pI)

49.54

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
DUF3527	PF12043	597 - 703	1.3e-06	Domain of unknown function (DUF3527)
DUF3527	PF12043	709 - 880	2.3e-39	Domain of unknown function (DUF3527)

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0008676)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT5G01030 AT5G01030 AT5G01030
fragaria_vesca	FvH4_6g12500 FvH4_6g12500
malus_domestica	MD04G1141700.v1.1 MD12G1156300.v1.1
prunus_persica	Prupe.6G267100_v2.0.a1
pyrus_communis	pycom04g12880 pycom12g14840
rosa_chinensis	RchiOBHm_Chr3g0464321
rosa_laevigata	RLG00000024708
rosa_multiflora	Rmu_sc0003074.1_g000030 Rmu_sc0007811.1_g000001 Rmu_sc0029720.1_g000001
rosa_roxburghii	Rroxscaffold_6G00415810
rosa_rugosa	Rorug03G0071300 Rorug03G0071300 Rorug03G0071400
rosa_samantha	Rh3BG133500 Rh3CG135800 Rh3DG135000
rosa_wichuraiana	Rw3G010940

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AarI	CACCTGC	1 cut(s)	131
Acc36I	ACCTGC	2 cut(s)	131, 366
Acc65I	GGTACC	1 cut(s)	1216
AccB1I	GGYRCC	2 cut(s)	1216, 2554
AccI	GTMKAC	1 cut(s)	322
AccII	CGCG	1 cut(s)	460
AccIII	TCCGGA	2 cut(s)	1556, 2491
AciI	CCGC	6 cut(s)	372, 692, 968, 1508, 1868, 2475
AclWI	GGATC	4 cut(s)	409, 1213, 1395, 1548
AcsI	RAATTY	5 cut(s)	839, 1319, 1882, 2585, 2710
AcuI	CTGAAG	5 cut(s)	657, 1176, 1438, 1710, 1790
AdeI	CACNNNGTG	1 cut(s)	503
AfaI	GTAC	6 cut(s)	10, 1218, 1326, 1603, 2148, 2299
AflII	CTTAAG	1 cut(s)	2068
AflIII	ACRYGT	4 cut(s)	64, 231, 2152, 2592
AhlI	ACTAGT	1 cut(s)	47
AjiI	CACGTC	1 cut(s)	2595
AjnI	CCWGG	6 cut(s)	537, 596, 2314, 2557, 2700, 2733
AjuI	GAANNNNNNNTTGG	6 cut(s)	381, 413, 1002, 1034, 2455, 2487
Alw21I	GWGCWC	3 cut(s)	829, 2350, 2527
Alw26I	GTCTC	3 cut(s)	483, 606, 1095
AlwI	GGATC	4 cut(s)	409, 1213, 1395, 1548
AlwNI	CAGNNNCTG	3 cut(s)	1079, 1547, 1768
Ama87I	CYCGRG	1 cut(s)	1096
Aor13HI	TCCGGA	2 cut(s)	1556, 2491
AoxI	GGCC	3 cut(s)	490, 2537, 2744
ApeKI	GCWGC	9 cut(s)	37, 103, 203, 689, 965, 2165, 2168, 2247, 2422
ApoI	RAATTY	5 cut(s)	839, 1319, 1882, 2585, 2710
ArsI	GACNNNNNNTTYG	2 cut(s)	501, 533
AseI	ATTAAT	2 cut(s)	1743, 1935
Asp700I	GAANNNNTTC	2 cut(s)	652, 2696
Asp718I	GGTACC	1 cut(s)	1216
AspA2I	CCTAGG	1 cut(s)	2186
AspLEI	GCGC	4 cut(s)	42, 534, 1278, 2252
AspS9I	GGNCC	5 cut(s)	157, 725, 1027, 1544, 2744
AsuC2I	CCSGG	1 cut(s)	246
AsuHPI	GGTGA	6 cut(s)	94, 155, 2127, 2325, 2346, 2749
AsuNHI	GCTAGC	1 cut(s)	1405
AvaI	CYCGRG	1 cut(s)	1096
AvaII	GGWCC	4 cut(s)	157, 725, 1027, 1544
AvrII	CCTAGG	1 cut(s)	2186
BanI	GGYRCC	2 cut(s)	1216, 2554
BanII	GRGCYC	2 cut(s)	829, 2527
BbsI	GAAGAC	1 cut(s)	162
Bbv12I	GWGCWC	3 cut(s)	829, 2350, 2527
BbvCI	CCTCAGC	2 cut(s)	1776, 2514
BbvI	GCAGC	9 cut(s)	49, 115, 215, 701, 952, 2152, 2155, 2234, 2409
BccI	CCATC	9 cut(s)	140, 520, 746, 838, 1000, 1114, 1446, 2561, 2662
BceAI	ACGGC	1 cut(s)	1481
BciT130I	CCWGG	6 cut(s)	539, 598, 2316, 2559, 2702, 2735
BciVI	GTATCC	1 cut(s)	2488
BclI	TGATCA	1 cut(s)	799
BcnI	CCSGG	1 cut(s)	246
BcoDI	GTCTC	3 cut(s)	483, 606, 1095
BcuI	ACTAGT	1 cut(s)	47
BfmI	CTRYAG	2 cut(s)	1185, 2301
BfoI	RGCGCY	1 cut(s)	43
BfrI	CTTAAG	1 cut(s)	2068
BfuAI	ACCTGC	2 cut(s)	131, 366
BfuI	GTATCC	1 cut(s)	2488
BglII	AGATCT	2 cut(s)	2125, 2659
BlnI	CCTAGG	1 cut(s)	2186
BlpI	GCTNAGC	1 cut(s)	444
BmcAI	AGTACT	2 cut(s)	1603, 2299
Bme1390I	CCNGG	7 cut(s)	246, 539, 598, 2316, 2559, 2702, 2735
Bme18I	GGWCC	4 cut(s)	157, 725, 1027, 1544
BmeT110I	CYCGRG	1 cut(s)	1096
BmgBI	CACGTC	1 cut(s)	2595
BmgT120I	GGNCC	5 cut(s)	157, 725, 1027, 1544, 2744
BmiI	GGNNCC	3 cut(s)	1218, 2556, 2706
BmrFI	CCNGG	7 cut(s)	246, 539, 598, 2316, 2559, 2702, 2735
BmsI	GCATC	7 cut(s)	127, 190, 618, 844, 1061, 1790, 1805
BmtI	GCTAGC	1 cut(s)	1409
BpiI	GAAGAC	1 cut(s)	162
BplI	GAGNNNNNCTC	2 cut(s)	437, 469
BpmI	CTGGAG	1 cut(s)	1016
Bpu10I	CCTNAGC	3 cut(s)	1776, 2514, 2526
Bpu1102I	GCTNAGC	1 cut(s)	444
BpuEI	CTTGAG	1 cut(s)	2218
BpuMI	CCSGG	1 cut(s)	246
BsaI	GGTCTC	1 cut(s)	606
BsaJI	CCNNGG	6 cut(s)	384, 596, 1113, 2186, 2265, 2558
BsaWI	WCCGGW	2 cut(s)	1556, 2491
BsaXI	ACNNNNNCTCC	2 cut(s)	1287, 1317
Bse118I	RCCGGY	2 cut(s)	1443, 2746
Bse1I	ACTGG	2 cut(s)	94, 1912
Bse3DI	GCAATG	1 cut(s)	436
BseAI	TCCGGA	2 cut(s)	1556, 2491
BseBI	CCWGG	6 cut(s)	539, 598, 2316, 2559, 2702, 2735
BseDI	CCNNGG	6 cut(s)	384, 596, 1113, 2186, 2265, 2558
BseGI	GGATG	8 cut(s)	380, 757, 1066, 1906, 1971, 2261, 2413, 2572
BseMI	GCAATG	1 cut(s)	436
BseMII	CTCAG	7 cut(s)	435, 633, 854, 894, 1767, 2505, 2540
BseNI	ACTGG	2 cut(s)	94, 1912
BseRI	GAGGAG	2 cut(s)	285, 2531
BseXI	GCAGC	9 cut(s)	49, 115, 215, 701, 952, 2152, 2155, 2234, 2409
BseYI	CCCAGC	3 cut(s)	1264, 2286, 2403
BsgI	GTGCAG	2 cut(s)	56, 1266
Bsh1236I	CGCG	1 cut(s)	460
BshFI	GGCC	3 cut(s)	492, 2539, 2746
BshNI	GGYRCC	2 cut(s)	1216, 2554
BsiHKAI	GWGCWC	3 cut(s)	829, 2350, 2527
BsiHKCI	CYCGRG	1 cut(s)	1096
BsiSI	CCGG	5 cut(s)	246, 1444, 1557, 2492, 2747
BslFI	GGGAC	4 cut(s)	448, 1247, 1715, 2761
BsmAI	GTCTC	3 cut(s)	483, 606, 1095
BsmBI	CGTCTC	1 cut(s)	483
BsmFI	GGGAC	4 cut(s)	448, 1247, 1715, 2761
BsmI	GAATGC	2 cut(s)	568, 691
BsnI	GGCC	3 cut(s)	492, 2539, 2746
Bso31I	GGTCTC	1 cut(s)	606
BsoBI	CYCGRG	1 cut(s)	1096
Bsp1286I	GDGCHC	3 cut(s)	829, 2350, 2527
Bsp13I	TCCGGA	2 cut(s)	1556, 2491
Bsp1407I	TGTACA	1 cut(s)	1324
Bsp143I	GATC	9 cut(s)	401, 799, 1086, 1205, 1387, 1553, 2110, 2125, 2659
Bsp1720I	GCTNAGC	1 cut(s)	444
BspACI	CCGC	6 cut(s)	372, 692, 968, 1508, 1868, 2475
BspANI	GGCC	3 cut(s)	492, 2539, 2746
BspCNI	CTCAG	7 cut(s)	436, 632, 855, 895, 1768, 2506, 2539
BspEI	TCCGGA	2 cut(s)	1556, 2491
BspFNI	CGCG	1 cut(s)	460
BspLI	GGNNCC	3 cut(s)	1218, 2556, 2706
BspMI	ACCTGC	2 cut(s)	131, 366
BspOI	GCTAGC	1 cut(s)	1409
BspPI	GGATC	4 cut(s)	409, 1213, 1395, 1548
BspT107I	GGYRCC	2 cut(s)	1216, 2554
BspTI	CTTAAG	1 cut(s)	2068
BspTNI	GGTCTC	1 cut(s)	606
BsrDI	GCAATG	1 cut(s)	436
BsrFI	RCCGGY	2 cut(s)	1443, 2746
BsrGI	TGTACA	1 cut(s)	1324
BsrI	ACTGG	2 cut(s)	94, 1912
BssAI	RCCGGY	2 cut(s)	1443, 2746
BssECI	CCNNGG	6 cut(s)	384, 596, 1113, 2186, 2265, 2558
BssMI	GATC	9 cut(s)	401, 799, 1086, 1205, 1387, 1553, 2110, 2125, 2659
BssT1I	CCWWGG	3 cut(s)	384, 1113, 2186
Bst2UI	CCWGG	6 cut(s)	539, 598, 2316, 2559, 2702, 2735
Bst4CI	ACNGT	8 cut(s)	979, 1238, 1337, 1534, 1571, 1862, 1931, 2369
Bst6I	CTCTTC	1 cut(s)	460
BstAFI	CTTAAG	1 cut(s)	2068
BstAPI	GCANNNNNTGC	1 cut(s)	2756
BstAUI	TGTACA	1 cut(s)	1324
BstC8I	GCNNGC	3 cut(s)	1407, 2163, 2748
BstDEI	CTNAG	8 cut(s)	444, 619, 863, 903, 1459, 1776, 2514, 2526
BstENI	CCTNNNNNAGG	4 cut(s)	267, 388, 497, 595
BstF5I	GGATG	8 cut(s)	380, 757, 1066, 1906, 1971, 2261, 2413, 2572
BstFNI	CGCG	1 cut(s)	460
BstH2I	RGCGCY	1 cut(s)	43
BstHHI	GCGC	4 cut(s)	42, 534, 1278, 2252
BstKTI	GATC	9 cut(s)	404, 802, 1089, 1208, 1390, 1556, 2113, 2128, 2662
BstMAI	GTCTC	3 cut(s)	483, 606, 1095
BstMBI	GATC	9 cut(s)	401, 799, 1086, 1205, 1387, 1553, 2110, 2125, 2659
BstMWI	GCNNNNNNNGC	3 cut(s)	226, 2283, 2756
BstNI	CCWGG	6 cut(s)	539, 598, 2316, 2559, 2702, 2735
BstNSI	RCATGY	6 cut(s)	68, 235, 1276, 2156, 2429, 2481
BstSCI	CCNGG	7 cut(s)	244, 537, 596, 2314, 2557, 2700, 2733
BstSFI	CTRYAG	2 cut(s)	1185, 2301
BstUI	CGCG	1 cut(s)	460
BstV1I	GCAGC	9 cut(s)	49, 115, 215, 701, 952, 2152, 2155, 2234, 2409
BstV2I	GAAGAC	1 cut(s)	162
BstX2I	RGATCY	3 cut(s)	1553, 2125, 2659
BstXI	CCANNNNNNTGG	1 cut(s)	1120
BstYI	RGATCY	3 cut(s)	1553, 2125, 2659
BsuI	GTATCC	1 cut(s)	2488
BsuRI	GGCC	3 cut(s)	492, 2539, 2746
BtgZI	GCGATG	2 cut(s)	816, 1424
BtrI	CACGTC	1 cut(s)	2595
BtsCI	GGATG	8 cut(s)	380, 757, 1066, 1906, 1971, 2261, 2413, 2572
BtsI	GCAGTG	2 cut(s)	955, 2222
BtsIMutI	CAGTG	2 cut(s)	955, 2222
BveI	ACCTGC	2 cut(s)	131, 366
Cac8I	GCNNGC	3 cut(s)	1407, 2163, 2748
CaiI	CAGNNNCTG	3 cut(s)	1079, 1547, 1768
CfoI	GCGC	4 cut(s)	42, 534, 1278, 2252
Cfr10I	RCCGGY	2 cut(s)	1443, 2746
Cfr13I	GGNCC	5 cut(s)	157, 725, 1027, 1544, 2744
CseI	GACGC	1 cut(s)	2131
Csp6I	GTAC	6 cut(s)	9, 1217, 1325, 1602, 2147, 2298
CviQI	GTAC	6 cut(s)	9, 1217, 1325, 1602, 2147, 2298
DdeI	CTNAG	8 cut(s)	444, 619, 863, 903, 1459, 1776, 2514, 2526
DpnI	GATC	9 cut(s)	403, 801, 1088, 1207, 1389, 1555, 2112, 2127, 2661
DpnII	GATC	9 cut(s)	401, 799, 1086, 1205, 1387, 1553, 2110, 2125, 2659
DraIII	CACNNNGTG	1 cut(s)	503
Eam1104I	CTCTTC	1 cut(s)	460
EarI	CTCTTC	1 cut(s)	460
Ecl136II	GAGCTC	2 cut(s)	827, 2525
Eco130I	CCWWGG	3 cut(s)	384, 1113, 2186
Eco24I	GRGCYC	2 cut(s)	829, 2527
Eco31I	GGTCTC	1 cut(s)	606
Eco32I	GATATC	3 cut(s)	588, 781, 2272
Eco47I	GGWCC	4 cut(s)	157, 725, 1027, 1544
Eco53kI	GAGCTC	2 cut(s)	827, 2525
Eco57I	CTGAAG	5 cut(s)	657, 1176, 1438, 1710, 1790
Eco88I	CYCGRG	1 cut(s)	1096
EcoICRI	GAGCTC	2 cut(s)	827, 2525
EcoNI	CCTNNNNNAGG	4 cut(s)	267, 388, 497, 595
EcoO109I	RGGNCCY	2 cut(s)	1027, 1544
EcoRI	GAATTC	1 cut(s)	2585
EcoRII	CCWGG	6 cut(s)	537, 596, 2314, 2557, 2700, 2733
EcoRV	GATATC	3 cut(s)	588, 781, 2272
EcoT14I	CCWWGG	3 cut(s)	384, 1113, 2186
EcoT38I	GRGCYC	2 cut(s)	829, 2527
ErhI	CCWWGG	3 cut(s)	384, 1113, 2186
Esp3I	CGTCTC	1 cut(s)	483
FalI	AAGNNNNNCTT	6 cut(s)	575, 607, 1076, 1108, 1889, 1921
FaqI	GGGAC	4 cut(s)	448, 1247, 1715, 2761
FbaI	TGATCA	1 cut(s)	799
FblI	GTMKAC	1 cut(s)	322
FokI	GGATG	8 cut(s)	367, 764, 1073, 1913, 1978, 2248, 2420, 2579
FriOI	GRGCYC	2 cut(s)	829, 2527
GlaI	GCGC	4 cut(s)	41, 533, 1277, 2251
GsaI	CCCAGC	3 cut(s)	1268, 2290, 2407
GsuI	CTGGAG	1 cut(s)	1016
HaeII	RGCGCY	1 cut(s)	43
HaeIII	GGCC	3 cut(s)	492, 2539, 2746
HapII	CCGG	5 cut(s)	246, 1444, 1557, 2492, 2747
HgaI	GACGC	1 cut(s)	2131
HhaI	GCGC	4 cut(s)	42, 534, 1278, 2252
Hin6I	GCGC	4 cut(s)	40, 532, 1276, 2250
HinP1I	GCGC	4 cut(s)	40, 532, 1276, 2250
HincII	GTYRAC	4 cut(s)	323, 1192, 1849, 2056
HindII	GTYRAC	4 cut(s)	323, 1192, 1849, 2056
HpaI	GTTAAC	1 cut(s)	2056
HpaII	CCGG	5 cut(s)	246, 1444, 1557, 2492, 2747
HphI	GGTGA	6 cut(s)	94, 155, 2127, 2325, 2346, 2749
Hpy166II	GTNNAC	5 cut(s)	157, 323, 1192, 1849, 2056
Hpy8I	GTNNAC	5 cut(s)	157, 323, 1192, 1849, 2056
Hpy99I	CGWCG	1 cut(s)	2147
HpyCH4III	ACNGT	8 cut(s)	979, 1238, 1337, 1534, 1571, 1862, 1931, 2369
HpyCH4IV	ACGT	1 cut(s)	2594
HpyF10VI	GCNNNNNNNGC	3 cut(s)	226, 2283, 2756
HpyF3I	CTNAG	8 cut(s)	444, 619, 863, 903, 1459, 1776, 2514, 2526
HpySE526I	ACGT	1 cut(s)	2594
HspAI	GCGC	4 cut(s)	40, 532, 1276, 2250
Kpn2I	TCCGGA	2 cut(s)	1556, 2491
KpnI	GGTACC	1 cut(s)	1220
KroI	GCCGGC	1 cut(s)	2746
KroNI	GCCGGC	1 cut(s)	2748
Ksp22I	TGATCA	1 cut(s)	799
KspAI	GTTAAC	1 cut(s)	2056
Kzo9I	GATC	9 cut(s)	401, 799, 1086, 1205, 1387, 1553, 2110, 2125, 2659
LmnI	GCTCC	7 cut(s)	824, 832, 2042, 2158, 2345, 2522, 2764
Lsp1109I	GCAGC	9 cut(s)	49, 115, 215, 701, 952, 2152, 2155, 2234, 2409
LweI	GCATC	7 cut(s)	127, 190, 618, 844, 1061, 1790, 1805
MaeII	ACGT	1 cut(s)	2594
MaeIII	GTNAC	8 cut(s)	1638, 2181, 2219, 2480, 2595, 2607, 2675, 2737
MalI	GATC	9 cut(s)	403, 801, 1088, 1207, 1389, 1555, 2112, 2127, 2661
MboI	GATC	9 cut(s)	401, 799, 1086, 1205, 1387, 1553, 2110, 2125, 2659
MfeI	CAATTG	1 cut(s)	882
MflI	RGATCY	3 cut(s)	1553, 2125, 2659
MhlI	GDGCHC	3 cut(s)	829, 2350, 2527
MlyI	GAGTC	5 cut(s)	536, 1763, 1978, 2331, 2467
MmeI	TCCRAC	2 cut(s)	2064, 2351
MroI	TCCGGA	2 cut(s)	1556, 2491
MroNI	GCCGGC	1 cut(s)	2746
MroXI	GAANNNNTTC	2 cut(s)	652, 2696
MslI	CAYNNNNRTG	2 cut(s)	1168, 1526
MspA1I	CMGCKG	2 cut(s)	692, 2286
MspCI	CTTAAG	1 cut(s)	2068
MspI	CCGG	5 cut(s)	246, 1444, 1557, 2492, 2747
MspR9I	CCNGG	7 cut(s)	246, 539, 598, 2316, 2559, 2702, 2735
MunI	CAATTG	1 cut(s)	882
Mva1269I	GAATGC	2 cut(s)	568, 691
MvaI	CCWGG	6 cut(s)	539, 598, 2316, 2559, 2702, 2735
MvnI	CGCG	1 cut(s)	460
MwoI	GCNNNNNNNGC	3 cut(s)	226, 2283, 2756
NaeI	GCCGGC	1 cut(s)	2748
NciI	CCSGG	1 cut(s)	246
NdeII	GATC	9 cut(s)	401, 799, 1086, 1205, 1387, 1553, 2110, 2125, 2659
NgoMIV	GCCGGC	1 cut(s)	2746
NheI	GCTAGC	1 cut(s)	1405
NlaIV	GGNNCC	3 cut(s)	1218, 2556, 2706
NmuCI	GTSAC	3 cut(s)	2219, 2595, 2737
NspI	RCATGY	6 cut(s)	68, 235, 1276, 2156, 2429, 2481
PaqCI	CACCTGC	1 cut(s)	131
PciI	ACATGT	3 cut(s)	64, 231, 2152
PctI	GAATGC	2 cut(s)	568, 691
PdiI	GCCGGC	1 cut(s)	2748
PdmI	GAANNNNTTC	2 cut(s)	652, 2696
PfeI	GAWTC	7 cut(s)	773, 807, 1765, 2132, 2258, 2716, 2730
PfoI	TCCNGGA	2 cut(s)	537, 2700
PleI	GAGTC	5 cut(s)	536, 1762, 1977, 2330, 2466
PpsI	GAGTC	5 cut(s)	536, 1762, 1977, 2330, 2466
PpuMI	RGGWCCY	2 cut(s)	1027, 1544
PscI	ACATGT	3 cut(s)	64, 231, 2152
PshBI	ATTAAT	2 cut(s)	1743, 1935
Psp124BI	GAGCTC	2 cut(s)	829, 2527
Psp5II	RGGWCCY	2 cut(s)	1027, 1544
Psp6I	CCWGG	6 cut(s)	537, 596, 2314, 2557, 2700, 2733
PspFI	CCCAGC	3 cut(s)	1264, 2286, 2403
PspGI	CCWGG	6 cut(s)	537, 596, 2314, 2557, 2700, 2733
PspN4I	GGNNCC	3 cut(s)	1218, 2556, 2706
PspPI	GGNCC	5 cut(s)	157, 725, 1027, 1544, 2744
PspPPI	RGGWCCY	2 cut(s)	1027, 1544
PstNI	CAGNNNCTG	3 cut(s)	1079, 1547, 1768
PsuI	RGATCY	3 cut(s)	1553, 2125, 2659
PvuII	CAGCTG	1 cut(s)	2286
RsaI	GTAC	6 cut(s)	10, 1218, 1326, 1603, 2148, 2299
RsaNI	GTAC	6 cut(s)	9, 1217, 1325, 1602, 2147, 2298
RseI	CAYNNNNRTG	2 cut(s)	1168, 1526
SacI	GAGCTC	2 cut(s)	829, 2527
SalI	GTCGAC	1 cut(s)	321
Sau3AI	GATC	9 cut(s)	401, 799, 1086, 1205, 1387, 1553, 2110, 2125, 2659
Sau96I	GGNCC	5 cut(s)	157, 725, 1027, 1544, 2744
ScaI	AGTACT	2 cut(s)	1603, 2299
SchI	GAGTC	5 cut(s)	536, 1763, 1978, 2331, 2467
ScrFI	CCNGG	7 cut(s)	246, 539, 598, 2316, 2559, 2702, 2735
SduI	GDGCHC	3 cut(s)	829, 2350, 2527
SfaNI	GCATC	7 cut(s)	127, 190, 618, 844, 1061, 1790, 1805
SfcI	CTRYAG	2 cut(s)	1185, 2301
SinI	GGWCC	4 cut(s)	157, 725, 1027, 1544
SmiMI	CAYNNNNRTG	2 cut(s)	1168, 1526
SmlI	CTYRAG	2 cut(s)	2068, 2197
SmoI	CTYRAG	2 cut(s)	2068, 2197
SpeI	ACTAGT	1 cut(s)	47
SsiI	CCGC	6 cut(s)	372, 692, 968, 1508, 1868, 2475
SspI	AATATT	2 cut(s)	653, 1653
SstI	GAGCTC	2 cut(s)	829, 2527
StyD4I	CCNGG	7 cut(s)	244, 537, 596, 2314, 2557, 2700, 2733
StyI	CCWWGG	3 cut(s)	384, 1113, 2186
TaaI	ACNGT	8 cut(s)	979, 1238, 1337, 1534, 1571, 1862, 1931, 2369
TaiI	ACGT	1 cut(s)	2597
TaqI	TCGA	3 cut(s)	52, 322, 757
TatI	WGTACW	3 cut(s)	1324, 1601, 2297
TauI	GCSGC	3 cut(s)	375, 695, 1871
TfiI	GAWTC	7 cut(s)	773, 807, 1765, 2132, 2258, 2716, 2730
TscAI	CASTG	2 cut(s)	962, 2222
TseFI	GTSAC	3 cut(s)	2219, 2595, 2737
TseI	GCWGC	9 cut(s)	37, 103, 203, 689, 965, 2165, 2168, 2247, 2422
Tsp45I	GTSAC	3 cut(s)	2219, 2595, 2737
TspDTI	ATGAA	7 cut(s)	560, 1326, 1892, 1953, 2039, 2250, 2611
TspGWI	ACGGA	2 cut(s)	509, 830
TspRI	CASTG	2 cut(s)	962, 2222
Vha464I	CTTAAG	1 cut(s)	2068
VpaK11BI	GGWCC	4 cut(s)	157, 725, 1027, 1544
VspI	ATTAAT	2 cut(s)	1743, 1935
XagI	CCTNNNNNAGG	4 cut(s)	267, 388, 497, 595
XapI	RAATTY	5 cut(s)	839, 1319, 1882, 2585, 2710
XbaI	TCTAGA	1 cut(s)	2128
XceI	RCATGY	6 cut(s)	68, 235, 1276, 2156, 2429, 2481
XmaJI	CCTAGG	1 cut(s)	2186
XmiI	GTMKAC	1 cut(s)	322
XmnI	GAANNNNTTC	2 cut(s)	652, 2696
ZrmI	AGTACT	2 cut(s)	1603, 2299

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.