Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr04

Orthogroup ID

OG0010673

Physical Location & Seq

Genomic Coordinates Forward (+)

23091657 .. 23097022

Gene Structure 5366 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

MD04G1142000.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1476 bp

ATGGGAGCCCGCTGCTCCAGATTCTCCCTCTGCTGGTTCCACTCCCACCTTAAACCCACCGTCCTCGAATCTTCCGAACTGGACAATGGGGGCAAAAGCGACAAAAGCATATGGCCGACCTTCACGGAGTACAGCCTCGACCAAATGAAAGCCGCCACGTCTGGCTTCGCCTCCGACAACATAGTCTCCGAGCACGGCGAAAAAGCTCCCAACGTTGTCTACAAGGGAAAGCTCGACAACGGCCGGTTAATTGCCGTCAAGCGATTCAACAAGCTCGCCTGGCCCGACAATCGCCAATTCCTAGAGGAGGCGAGGTCGGTGGGGAGCCTGAGGAGCGAGAGGCTGGGGAATCTGATCGGATGCTGTTGCGAAGGGGAGGAGCGGCTGCTGGTGGCGGAGTTCATGGCGCACGAGACGCTGGCGAAGCACCTGTTCCACTGGGAGAGCCAGCCGATGAGGTGGGCGATGAGGCTGAGGGTGGCGCTGTATCTGGCTCAAGCTCTCGATTACTGCAGTGGGAAAGGTCGTGCCTTGTATCATGATCTCAATGCCTACAGGGTTTTGTTTGATCAGGATGGTAATCCCAGGCTGTCATGCTTTGGCCTCATGAAGAATAGCAGGGATGGCAAGAGTTACAGTACGAACTTGGCTTTCACTCCTCCTGAGTATCTCAGGACAGGTAGGGTGACGCAAGAAAGTGTAATTTACAGCTTTGGAACGTTGTTGCTTGATCTTCTAAGTGGCAAACATATTCCCCCCAGCCATGCGCTAGATCTAATTCGCGGCAAGAATTTCCTGATGCTAATGGATTCTGCTTTGGAGGGTAATTTCTCAAATGATGACGGAACTGAGTTAGTACGCTTAGCTTCCCGTTGTCTGCAATATGAAGCTCGTGAGCGGCCAAATGCAAAGTCCCTCGTCACTGCTCTCATGTCACTTCAGAAAGAAACAGAGGTTGCATCACATGTTTTGATGGGTATTGCACATGAAAGCGCATCCTCAACGCAGCCATTGGCATTAACACCCTTTGGAGAAGCTTGTTTGAGAATGGATCTCACTGCCATACATGAAATATTGGAAAAGAATGGATACAAGGATGATGAGGGGATTGCTAATGAGCTTTCTTTCCAGCTGTGGACTAATCAAATGCAGGAGACTTTGAATTCTAAGAAGAGTGGAGATTCTGCTTTTCGAGCTAAAGATTTTACAACTGCGATTGATTCCTATACACAATTTATCGACGGCGGGACCATGGTATCACCCACGGTGTATGCTCGGCGCTGCCTCTCTTACCTGATGAGTGAAATGCCACAAGAAGCTCTGGGGGATGCTATGCAAGCCCAGGTAGTTTCTCCCGAGTGGCCGGCGGCTTTGTACCTTCAAGCAGCTTGCCTCTTCAGCCTTGGCATGGATAATGATGCACAAGAAACACTCAAAGATGGCACAAATTTGGAAGCCAAAAAGAACAAAACTTGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0003824 GO:0004672 GO:0004674 GO:0004675 GO:0004888 GO:0005575 GO:0005623 GO:0005886 GO:0006464 GO:0006468 GO:0006793 GO:0006796 GO:0006807 GO:0007154 GO:0007165 GO:0007166 GO:0007167 GO:0007178 GO:0008150 GO:0008152 GO:0009719 GO:0009725 GO:0009741 GO:0009742 GO:0009755 GO:0009987 GO:0010033 GO:0014070 GO:0016020 GO:0016301 GO:0016310 GO:0016740 GO:0016772 GO:0016773 GO:0019199 GO:0019538 GO:0023052 GO:0032870 GO:0033993 GO:0036211 GO:0038023 GO:0042221 GO:0043170 GO:0043401 GO:0043412 GO:0044237 GO:0044238 GO:0044260 GO:0044267 GO:0044464 GO:0048545 GO:0050789 GO:0050794 GO:0050896 GO:0051716 GO:0060089 GO:0065007 GO:0070887 GO:0071310 GO:0071367 GO:0071383 GO:0071396 GO:0071407 GO:0071495 GO:0071704 GO:0071944 GO:0140096 GO:1901564 GO:1901700 GO:1901701

KEGG Pathways

Metabolic & Signaling

ko04075 map04075

Pfam Domains

Protein Families

Pkinase Pkinase_Tyr

Protein Analysis

492

Amino Acids

54.82

Weight (kDa)

5.9

Isoelectric Point (pI)

36.18

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
PK_Tyr_Ser-Thr	PF07714	72 - 310	3.9e-25	Protein tyrosine and serine/threonine kinase
Pkinase	PF00069	72 - 307	6.3e-21	Protein kinase domain
TPR_BSK1_C	PF25575	345 - 462	1.1e-64	Serine/threonine-protein kinase BSK1-like, TPR repeats

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0010673)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT3G54030
fragaria_vesca	FvH4_6g12470 FvH4_6g12470 FvH4_6g12470 FvH4_6g12470 FvH4_6g12470
malus_domestica	MD04G1142000.v1.1 MD12G1156600.v1.1
prunus_persica	Prupe.6G267400_v2.0.a1 Prupe.6G267400_v2.0.a1 Prupe.6G267400_v2.0.a1
pyrus_communis	pycom04g12900
rosa_chinensis	RchiOBHm_Chr3g0464291
rosa_laevigata	RLG00000024711
rosa_roxburghii	Rroxscaffold_6G00415840
rosa_rugosa	Rorug03G0071200 Rorug03G0071300
rosa_samantha	Rh3BG133200 Rh3CG135500
rosa_wichuraiana	Rw3G010910

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AasI	GACNNNNNNGTC	1 cut(s)	182
AccBSI	CCGCTC	2 cut(s)	382, 898
AccI	GTMKAC	1 cut(s)	219
AccII	CGCG	1 cut(s)	783
AciI	CCGC	8 cut(s)	10, 153, 382, 395, 783, 898, 1245, 1367
AclI	AACGTT	2 cut(s)	213, 719
AclWI	GGATC	1 cut(s)	1059
AcoI	YGGCCR	4 cut(s)	113, 241, 899, 1361
AcsI	RAATTY	3 cut(s)	790, 1162, 1447
AcuI	CTGAAG	2 cut(s)	923, 1381
AfaI	GTAC	4 cut(s)	131, 640, 858, 1376
AfiI	CCNNNNNNNGG	3 cut(s)	33, 307, 1408
AflIII	ACRYGT	1 cut(s)	964
AgsI	TTSAA	3 cut(s)	268, 1162, 1382
AjiI	CACGTC	1 cut(s)	159
AjnI	CCWGG	3 cut(s)	278, 584, 1341
Alw21I	GWGCWC	1 cut(s)	195
Alw26I	GTCTC	3 cut(s)	190, 407, 1148
AlwI	GGATC	1 cut(s)	1059
Ama87I	CYCGRG	1 cut(s)	1355
AoxI	GGCC	6 cut(s)	113, 241, 281, 601, 899, 1361
ApeKI	GCWGC	5 cut(s)	12, 385, 1006, 1281, 1385
ApoI	RAATTY	3 cut(s)	790, 1162, 1447
ArsI	GACNNNNNNTTYG	2 cut(s)	896, 928
AspLEI	GCGC	5 cut(s)	409, 484, 769, 995, 1281
AspS9I	GGNCC	2 cut(s)	282, 1248
AsuHPI	GGTGA	2 cut(s)	697, 1251
AvaI	CYCGRG	1 cut(s)	1355
AvaII	GGWCC	1 cut(s)	1248
AxyI	CCTNAGG	1 cut(s)	329
BaeI	ACNNNNGTAYC	2 cut(s)	1239, 1272
BanII	GRGCYC	1 cut(s)	10
BauI	CACGAG	2 cut(s)	410, 891
Bbv12I	GWGCWC	1 cut(s)	195
BbvCI	CCTCAGC	1 cut(s)	473
BbvI	GCAGC	4 cut(s)	372, 1018, 1268, 1397
BccI	CCATC	4 cut(s)	569, 617, 967, 1433
BceAI	ACGGC	4 cut(s)	211, 239, 256, 1258
BciT130I	CCWGG	3 cut(s)	280, 586, 1343
BciVI	GTATCC	1 cut(s)	1082
BclI	TGATCA	1 cut(s)	568
BcoDI	GTCTC	3 cut(s)	190, 407, 1148
BfaI	CTAG	2 cut(s)	302, 770
BfmI	CTRYAG	2 cut(s)	511, 553
BfoI	RGCGCY	2 cut(s)	485, 1282
BfuI	GTATCC	1 cut(s)	1082
BglII	AGATCT	1 cut(s)	772
BlpI	GCTNAGC	1 cut(s)	862
Bme1390I	CCNGG	3 cut(s)	280, 586, 1343
Bme18I	GGWCC	1 cut(s)	1248
BmeT110I	CYCGRG	1 cut(s)	1355
BmgBI	CACGTC	1 cut(s)	159
BmgT120I	GGNCC	2 cut(s)	282, 1248
BmiI	GGNNCC	4 cut(s)	7, 38, 326, 1249
BmrFI	CCNGG	3 cut(s)	280, 586, 1343
BmrI	ACTGGG	1 cut(s)	448
BmsI	GCATC	6 cut(s)	350, 789, 968, 1004, 1318, 1408
BmuI	ACTGGG	1 cut(s)	448
Bpu10I	CCTNAGC	1 cut(s)	473
Bpu1102I	GCTNAGC	1 cut(s)	862
BpuEI	CTTGAG	1 cut(s)	480
BsaBI	GATNNNNATC	1 cut(s)	579
BsaJI	CCNNGG	5 cut(s)	584, 1251, 1263, 1341, 1402
BsaXI	ACNNNNNCTCC	4 cut(s)	170, 200, 299, 329
Bsc4I	CCNNNNNNNGG	3 cut(s)	33, 307, 1408
Bse118I	RCCGGY	2 cut(s)	243, 1363
Bse1I	ACTGG	2 cut(s)	84, 443
Bse21I	CCTNAGG	1 cut(s)	329
Bse8I	GATNNNNATC	1 cut(s)	579
BseBI	CCWGG	3 cut(s)	280, 586, 1343
BseDI	CCNNGG	5 cut(s)	584, 1251, 1263, 1341, 1402
BseGI	GGATG	6 cut(s)	365, 580, 628, 995, 1102, 1333
BseJI	GATNNNNATC	1 cut(s)	579
BseLI	CCNNNNNNNGG	3 cut(s)	33, 307, 1408
BseMII	CTCAG	5 cut(s)	320, 464, 654, 685, 840
BseNI	ACTGG	2 cut(s)	84, 443
BseRI	GAGGAG	4 cut(s)	320, 346, 392, 648
BseX3I	CGGCCG	1 cut(s)	241
BseXI	GCAGC	4 cut(s)	372, 1018, 1268, 1397
BseYI	CCCAGC	2 cut(s)	343, 758
Bsh1236I	CGCG	1 cut(s)	783
Bsh1285I	CGRYCG	1 cut(s)	244
BshFI	GGCC	6 cut(s)	115, 243, 283, 603, 901, 1363
BsiEI	CGRYCG	1 cut(s)	244
BsiHKAI	GWGCWC	1 cut(s)	195
BsiHKCI	CYCGRG	1 cut(s)	1355
BsiSI	CCGG	2 cut(s)	244, 1364
BslFI	GGGAC	2 cut(s)	898, 1261
BslI	CCNNNNNNNGG	3 cut(s)	33, 307, 1408
BsmAI	GTCTC	3 cut(s)	190, 407, 1148
BsmBI	CGTCTC	1 cut(s)	407
BsmFI	GGGAC	2 cut(s)	898, 1261
BsnI	GGCC	6 cut(s)	115, 243, 283, 603, 901, 1363
BsoBI	CYCGRG	1 cut(s)	1355
Bsp1286I	GDGCHC	2 cut(s)	10, 195
Bsp143I	GATC	6 cut(s)	354, 541, 568, 730, 772, 1051
Bsp1720I	GCTNAGC	1 cut(s)	862
Bsp19I	CCATGG	1 cut(s)	1251
BspACI	CCGC	8 cut(s)	10, 153, 382, 395, 783, 898, 1245, 1367
BspANI	GGCC	6 cut(s)	115, 243, 283, 603, 901, 1363
BspCNI	CTCAG	5 cut(s)	321, 465, 655, 684, 841
BspFNI	CGCG	1 cut(s)	783
BspHI	TCATGA	2 cut(s)	538, 606
BspLI	GGNNCC	4 cut(s)	7, 38, 326, 1249
BspMAI	CTGCAG	1 cut(s)	515
BspPI	GGATC	1 cut(s)	1059
BsrBI	CCGCTC	2 cut(s)	382, 898
BsrFI	RCCGGY	2 cut(s)	243, 1363
BsrI	ACTGG	2 cut(s)	84, 443
BssAI	RCCGGY	2 cut(s)	243, 1363
BssECI	CCNNGG	5 cut(s)	584, 1251, 1263, 1341, 1402
BssMI	GATC	6 cut(s)	354, 541, 568, 730, 772, 1051
BssSI	CACGAG	2 cut(s)	410, 891
BssT1I	CCWWGG	2 cut(s)	1251, 1402
Bst2BI	CACGAG	2 cut(s)	410, 891
Bst2UI	CCWGG	3 cut(s)	280, 586, 1343
Bst4CI	ACNGT	3 cut(s)	61, 638, 1267
Bst6I	CTCTTC	2 cut(s)	1166, 1400
BstC8I	GCNNGC	7 cut(s)	10, 276, 420, 449, 1338, 1365, 1390
BstDEI	CTNAG	8 cut(s)	329, 473, 663, 671, 737, 849, 862, 1167
BstDSI	CCRYGG	2 cut(s)	1251, 1263
BstENI	CCTNNNNNAGG	1 cut(s)	305
BstF5I	GGATG	6 cut(s)	365, 580, 628, 995, 1102, 1333
BstFNI	CGCG	1 cut(s)	783
BstH2I	RGCGCY	2 cut(s)	485, 1282
BstHHI	GCGC	5 cut(s)	409, 484, 769, 995, 1281
BstKTI	GATC	6 cut(s)	357, 544, 571, 733, 775, 1054
BstMAI	GTCTC	3 cut(s)	190, 407, 1148
BstMBI	GATC	6 cut(s)	354, 541, 568, 730, 772, 1051
BstMCI	CGRYCG	1 cut(s)	244
BstMWI	GCNNNNNNNGC	9 cut(s)	105, 280, 333, 415, 424, 624, 1193, 1337, 1398
BstNI	CCWGG	3 cut(s)	280, 586, 1343
BstNSI	RCATGY	1 cut(s)	968
BstSCI	CCNGG	3 cut(s)	278, 584, 1341
BstSFI	CTRYAG	2 cut(s)	511, 553
BstUI	CGCG	1 cut(s)	783
BstV1I	GCAGC	4 cut(s)	372, 1018, 1268, 1397
BstX2I	RGATCY	2 cut(s)	772, 1051
BstYI	RGATCY	2 cut(s)	772, 1051
BstZI	CGGCCG	1 cut(s)	241
Bsu36I	CCTNAGG	1 cut(s)	329
BsuI	GTATCC	1 cut(s)	1082
BsuRI	GGCC	6 cut(s)	115, 243, 283, 603, 901, 1363
BtgI	CCRYGG	2 cut(s)	1251, 1263
BtgZI	GCGATG	1 cut(s)	479
BtrI	CACGTC	1 cut(s)	159
BtsCI	GGATG	6 cut(s)	365, 580, 628, 995, 1102, 1333
BtsI	GCAGTG	3 cut(s)	520, 921, 1056
BtsIMutI	CAGTG	4 cut(s)	436, 520, 921, 1056
Cac8I	GCNNGC	7 cut(s)	10, 276, 420, 449, 1338, 1365, 1390
CciI	TCATGA	2 cut(s)	538, 606
CfoI	GCGC	5 cut(s)	409, 484, 769, 995, 1281
Cfr10I	RCCGGY	2 cut(s)	243, 1363
Cfr13I	GGNCC	2 cut(s)	282, 1248
CseI	GACGC	2 cut(s)	424, 697
Csp6I	GTAC	4 cut(s)	130, 639, 857, 1375
CviQI	GTAC	4 cut(s)	130, 639, 857, 1375
DdeI	CTNAG	8 cut(s)	329, 473, 663, 671, 737, 849, 862, 1167
DpnI	GATC	6 cut(s)	356, 543, 570, 732, 774, 1053
DpnII	GATC	6 cut(s)	354, 541, 568, 730, 772, 1051
DrdI	GACNNNNNNGTC	1 cut(s)	182
DseDI	GACNNNNNNGTC	1 cut(s)	182
EaeI	YGGCCR	4 cut(s)	113, 241, 899, 1361
EagI	CGGCCG	1 cut(s)	241
Eam1104I	CTCTTC	2 cut(s)	1166, 1400
EarI	CTCTTC	2 cut(s)	1166, 1400
EciI	GGCGGA	1 cut(s)	410
EclXI	CGGCCG	1 cut(s)	241
Eco130I	CCWWGG	2 cut(s)	1251, 1402
Eco24I	GRGCYC	1 cut(s)	10
Eco47I	GGWCC	1 cut(s)	1248
Eco52I	CGGCCG	1 cut(s)	241
Eco57I	CTGAAG	2 cut(s)	923, 1381
Eco81I	CCTNAGG	1 cut(s)	329
Eco88I	CYCGRG	1 cut(s)	1355
EcoNI	CCTNNNNNAGG	1 cut(s)	305
EcoRI	GAATTC	1 cut(s)	1162
EcoRII	CCWGG	3 cut(s)	278, 584, 1341
EcoT14I	CCWWGG	2 cut(s)	1251, 1402
EcoT38I	GRGCYC	1 cut(s)	10
ErhI	CCWWGG	2 cut(s)	1251, 1402
Esp3I	CGTCTC	1 cut(s)	407
FaqI	GGGAC	2 cut(s)	898, 1261
FauI	CCCGC	2 cut(s)	17, 1238
FauNDI	CATATG	1 cut(s)	110
FbaI	TGATCA	1 cut(s)	568
FblI	GTMKAC	1 cut(s)	219
FokI	GGATG	6 cut(s)	372, 587, 635, 982, 1109, 1340
FriOI	GRGCYC	1 cut(s)	10
FspBI	CTAG	2 cut(s)	302, 770
GlaI	GCGC	5 cut(s)	408, 483, 768, 994, 1280
GsaI	CCCAGC	2 cut(s)	347, 762
HaeII	RGCGCY	2 cut(s)	485, 1282
HaeIII	GGCC	6 cut(s)	115, 243, 283, 603, 901, 1363
HapII	CCGG	2 cut(s)	244, 1364
HgaI	GACGC	2 cut(s)	424, 697
HhaI	GCGC	5 cut(s)	409, 484, 769, 995, 1281
Hin6I	GCGC	5 cut(s)	407, 482, 767, 993, 1279
HinP1I	GCGC	5 cut(s)	407, 482, 767, 993, 1279
HindIII	AAGCTT	1 cut(s)	1035
HinfI	GANTC	7 cut(s)	21, 68, 264, 349, 809, 1181, 1220
HpaII	CCGG	2 cut(s)	244, 1364
HphI	GGTGA	2 cut(s)	697, 1251
Hpy166II	GTNNAC	2 cut(s)	220, 1137
Hpy188I	TCNGA	6 cut(s)	76, 175, 190, 354, 359, 942
Hpy8I	GTNNAC	2 cut(s)	220, 1137
Hpy99I	CGWCG	1 cut(s)	1244
HpyAV	CCTTC	3 cut(s)	130, 365, 1388
HpyCH4III	ACNGT	3 cut(s)	61, 638, 1267
HpyCH4IV	ACGT	3 cut(s)	158, 213, 719
HpyCH4V	TGCA	8 cut(s)	513, 880, 908, 959, 983, 1150, 1336, 1421
HpyF10VI	GCNNNNNNNGC	9 cut(s)	105, 280, 333, 415, 424, 624, 1193, 1337, 1398
HpyF3I	CTNAG	8 cut(s)	329, 473, 663, 671, 737, 849, 862, 1167
HpySE526I	ACGT	3 cut(s)	158, 213, 719
HspAI	GCGC	5 cut(s)	407, 482, 767, 993, 1279
KroI	GCCGGC	1 cut(s)	1363
KroNI	GCCGGC	1 cut(s)	1365
Ksp22I	TGATCA	1 cut(s)	568
Kzo9I	GATC	6 cut(s)	354, 541, 568, 730, 772, 1051
LmnI	GCTCC	6 cut(s)	5, 20, 211, 324, 333, 379
Lsp1109I	GCAGC	4 cut(s)	372, 1018, 1268, 1397
LweI	GCATC	6 cut(s)	350, 789, 968, 1004, 1318, 1408
MaeI	CTAG	2 cut(s)	302, 770
MaeII	ACGT	3 cut(s)	158, 213, 719
MaeIII	GTNAC	4 cut(s)	632, 685, 919, 933
MalI	GATC	6 cut(s)	356, 543, 570, 732, 774, 1053
MbiI	CCGCTC	2 cut(s)	382, 898
MboI	GATC	6 cut(s)	354, 541, 568, 730, 772, 1051
MboII	GAAGA	5 cut(s)	63, 622, 725, 1183, 1387
MflI	RGATCY	2 cut(s)	772, 1051
MhlI	GDGCHC	2 cut(s)	10, 195
MluCI	AATT	9 cut(s)	249, 296, 702, 777, 790, 826, 1162, 1232, 1447
MmeI	TCCRAC	1 cut(s)	198
MroNI	GCCGGC	1 cut(s)	1363
MseI	TTAA	3 cut(s)	51, 248, 1019
MspA1I	CMGCKG	2 cut(s)	12, 1132
MspI	CCGG	2 cut(s)	244, 1364
MspR9I	CCNGG	3 cut(s)	280, 586, 1343
MvaI	CCWGG	3 cut(s)	280, 586, 1343
MvnI	CGCG	1 cut(s)	783
MwoI	GCNNNNNNNGC	9 cut(s)	105, 280, 333, 415, 424, 624, 1193, 1337, 1398
NaeI	GCCGGC	1 cut(s)	1365
NcoI	CCATGG	1 cut(s)	1251
NdeI	CATATG	1 cut(s)	110
NdeII	GATC	6 cut(s)	354, 541, 568, 730, 772, 1051
NgoMIV	GCCGGC	1 cut(s)	1363
NlaIV	GGNNCC	4 cut(s)	7, 38, 326, 1249
NmeAIII	GCCGAG	1 cut(s)	1255
NmuCI	GTSAC	3 cut(s)	685, 919, 933
NspI	RCATGY	1 cut(s)	968
PagI	TCATGA	2 cut(s)	538, 606
PciI	ACATGT	1 cut(s)	964
PcsI	WCGNNNNNNNCGW	2 cut(s)	72, 282
PdiI	GCCGGC	1 cut(s)	1365
PfeI	GAWTC	7 cut(s)	21, 68, 264, 349, 809, 1181, 1220
PscI	ACATGT	1 cut(s)	964
Psp1406I	AACGTT	2 cut(s)	213, 719
Psp6I	CCWGG	3 cut(s)	278, 584, 1341
PspFI	CCCAGC	2 cut(s)	343, 758
PspGI	CCWGG	3 cut(s)	278, 584, 1341
PspN4I	GGNNCC	4 cut(s)	7, 38, 326, 1249
PspPI	GGNCC	2 cut(s)	282, 1248
PstI	CTGCAG	1 cut(s)	515
PsuI	RGATCY	2 cut(s)	772, 1051
PvuII	CAGCTG	1 cut(s)	1132
RsaI	GTAC	4 cut(s)	131, 640, 858, 1376
RsaNI	GTAC	4 cut(s)	130, 639, 857, 1375
SaqAI	TTAA	3 cut(s)	51, 248, 1019
Sau3AI	GATC	6 cut(s)	354, 541, 568, 730, 772, 1051
Sau96I	GGNCC	2 cut(s)	282, 1248
ScrFI	CCNGG	3 cut(s)	280, 586, 1343
SduI	GDGCHC	2 cut(s)	10, 195
SfaNI	GCATC	6 cut(s)	350, 789, 968, 1004, 1318, 1408
SfcI	CTRYAG	2 cut(s)	511, 553
SinI	GGWCC	1 cut(s)	1248
SmlI	CTYRAG	1 cut(s)	495
SmoI	CTYRAG	1 cut(s)	495
Sse9I	AATT	9 cut(s)	249, 296, 702, 777, 790, 826, 1162, 1232, 1447
SsiI	CCGC	8 cut(s)	10, 153, 382, 395, 783, 898, 1245, 1367
SspI	AATATT	1 cut(s)	1074
SspMI	CTAG	2 cut(s)	302, 770
StyD4I	CCNGG	3 cut(s)	278, 584, 1341
StyI	CCWWGG	2 cut(s)	1251, 1402
TaaI	ACNGT	3 cut(s)	61, 638, 1267
TaiI	ACGT	3 cut(s)	161, 216, 722
TaqI	TCGA	6 cut(s)	66, 138, 234, 504, 1192, 1239
TasI	AATT	9 cut(s)	249, 296, 702, 777, 790, 826, 1162, 1232, 1447
TatI	WGTACW	1 cut(s)	129
TauI	GCSGC	5 cut(s)	155, 385, 786, 901, 1370
TfiI	GAWTC	7 cut(s)	21, 68, 264, 349, 809, 1181, 1220
Tru1I	TTAA	3 cut(s)	51, 248, 1019
Tru9I	TTAA	3 cut(s)	51, 248, 1019
TscAI	CASTG	4 cut(s)	443, 520, 928, 1063
TseFI	GTSAC	3 cut(s)	685, 919, 933
TseI	GCWGC	5 cut(s)	12, 385, 1006, 1281, 1385
Tsp45I	GTSAC	3 cut(s)	685, 919, 933
TspDTI	ATGAA	6 cut(s)	161, 391, 623, 900, 1002, 1083
TspGWI	ACGGA	2 cut(s)	140, 858
TspRI	CASTG	4 cut(s)	443, 520, 928, 1063
VpaK11BI	GGWCC	1 cut(s)	1248
XagI	CCTNNNNNAGG	1 cut(s)	305
XapI	RAATTY	3 cut(s)	790, 1162, 1447
XceI	RCATGY	1 cut(s)	968
XmiI	GTMKAC	1 cut(s)	219
XspI	CTAG	2 cut(s)	302, 770

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.