Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr04

Orthogroup ID

OG0008815

Physical Location & Seq

Genomic Coordinates Forward (+)

23486050 .. 23491474

Gene Structure 5425 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

MD04G1146400.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 2286 bp

ATGGCTTCGATATCTCATTCTCCACTGGCGTTTGCTGGAACAAGCACCGCTTCGGATCTTTTTCGGAGCTCCAGTAATGGCGTCGGCGGTGTTCCGTTGAGAGCTTTAGGGAAGGTGCGGTTGGGCATGAGGAGGAACGTTGTGTTGGCCGCCAAGCTCAGGAAGGTGAAGAAGCACGAGTACCCCTGGCCTGAGGATCCCGATCCGAATGTCAAAGGTGGAGTGCTCACTCATCTCTCTCACTTCAAGCCGCTGAAGGAGAAGCCCAAGCCGGTTACCTTGCCGTTTGAGAAGCCGCTTGTTGATATCGAAAAGAAGATCATTGATGTGCGGAAGATGGCGAACGAAACTGGGCTGGACTTCAGTGATCAAATTATTTCACTGGAGAATAAATACAACCAGGCTTTAAAGGATTTATATACGAATCTGACTCCTATACAACGTGTGAATATTGCACGACATCCAAACAGGCCAACTTTCCTTGACCATGTGTTTAACATTACTGATAAGTTTGTAGAGCTTCATGGGGACCGAGCAGGGTATGATGATCCTGCTATTGTCACTGGTATAGGAACTATAGATGGTAGAAGGTATATGTTCATGGGTCACCAGAAAGGTAGAAATACGAAGGAGAATATTATGCGTAACTTTGGGATGCCTACTCCTCACGGTTACCGGAAAGCTCTGCGCATGATGTACTATGCAGATCACCATGGTTTCCCAATTGTTACTTTCATCGATACGCCTGGTGCATATGCAGACCTTAAATCTGAGGAACTGGGCCAAGGTGAAGCCATAGCTCACAACTTGCGTACTATGTTTGGTCTGAAAGTACCTATTGTCTCTATTGTCATGGGAGAAGGTGGCTCTGGTGGTGCCCTTGCCATTGGCTGTGCTAATACATTACTAATGCTTGAAAATGCAGTTTTCTATGTTGCCAGTCCAGAAGCATGTGCAGCAATCTTGTGGAAGAGTTCTAAAGCTTCTCCAAAGGCTGCTGAAAAGCTGAAAATTACTGCAACTGAGTTGTGCAAACTGCAAATTGCAGATGGCATCATCCCTGAGCCGCTTGGCGGCGCACATGCGGATCCTGCATGGACCTCTCAACAGATCAAAAATGCAATTAACAAAGAAATGGATGAGCTCCATAAGTTGGACACAGAAGCATTACTAAAGCATCGCATGATGAAGTTCCGGAAAATTGGTGGGTTCCAAGAAGGAATTCCTATAGATCCAAAGAAGAAAAAGAACATGAAACCAAAAGAGCAGCCTGTCTATGTGAAGACTCCGGTTGTAGATTTGGAGGGTGAGGTTGAAAAGGTAAAACAGCAAATCTTGAAAGCCAAGGAATCTTCTAATGAGCCTCCATTGGTGCCACTGACTGAGACGATAAAAAAACTGAGAAAGGAAGTGGATACTGAATTTTCTGAGGCAGTTAAATCCTTGGGTTTGAAAGAGAGGTTTGCCACTTTGCGAGAAGAATTTGCAAAAGTAAATGCACAGAACCAACTCCTGCATCCAGCTCTAAAAGCAAAGCTTGATAAGCTTAGGGAGGAATTCAACAAAGGGCTGCTGACAGCTCCTAATTATGAAGACCTAAAATATAAACTCGACATGTTGAAGGAATTGTCAAAAGCATATGATCTTGCCGAGAGCAACAAGAAGGCTGCAAAACTGAAGCAGGAAGTTAACAAGAAATTCAGCGAGATCATGGATCGGGAAGATGTTAAGGAGAAGGTTGTGGCACTTAGGGTTGAGGTTGAAAACTCTGGGGTGTCTAAGTTTGATGATTTGGATGACAGCCTGAGGGAGAAAATCAAGGAATTGAAGAAAGAGCTGGAGTTTGAATTTATTGATGTCCTCAAGTCATTGGGTTTGGATGTTGAATTAAAAGCAAAAGAGCCCGTTGAGCAAACTTTGCCCTCAGAAGTTAAGAACAAGATCGAAGAATTGAATGGAGAAATTAATGAGAGGATTGAAAGTGTGATCAATACAACAGACCTGAAGGACAAAGTTGAGCTACTGAAGTTGGAGATAGCAAAGGCAGGGAAAACACCCGATTCAGCAGCCAAAAATAGAATCGTGGCATTGGAGCAACAAATCAAACAAAGCTTAGCAGCAGCTGTGGAATCTTCAAATTTAAAAGAGAAGCATGAGAAGCTGAAGGCCGAGATTTCCCAAACTGTTGGATCGAATGAAAGTCTGAAGAAGGACGATGAAGACTCTTTCTCCTTTGATGCATCTAGAGTAGTGGGTGCAAATCGCACGTTCGTACTTCTCTTTTAG

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0009507 GO:0009526 GO:0009532 GO:0009536 GO:0009570 GO:0009941 GO:0016020 GO:0031967 GO:0031975 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044422 GO:0044424 GO:0044434 GO:0044435 GO:0044444 GO:0044446 GO:0044464

KEGG Pathways

Metabolic & Signaling

ko00061 ko00620 ko00640 ko00720 ko01100 ko01110 ko01120 ko01130 ko01200 ko01212 map00061 map00620 map00640 map00720 map01100 map01110 map01120 map01130 map01200 map01212

Pfam Domains

Protein Families

ACCA

Protein Analysis

762

Amino Acids

85.07

Weight (kDa)

8.84

Isoelectric Point (pI)

34.52

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
ACCA	PF03255	94 - 402	5.4e-131	Acetyl co-enzyme A carboxylase carboxyltransferase-like
Carboxyl_trans	PF01039	171 - 333	7.2e-08	Carboxyl transferase domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0008815)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT2G38040 AT2G38040
fragaria_vesca	FvH4_6g12150 FvH4_6g12150 FvH4_6g12150 FvH4_6g12150
malus_domestica	MD04G1146400.v1.1 MD12G1159600.v1.1
prunus_persica	Prupe.6G270200_v2.0.a1
pyrus_communis	pycom04g13200 pycom12g15130
rosa_chinensis	RchiOBHm_Chr3g0463941
rosa_laevigata	RLG00000024733
rosa_multiflora	Rmu_co8513843.1_g000002 Rmu_sc0006492.1_g000005
rosa_roxburghii	Rroxscaffold_6G00416120
rosa_rugosa	Rorug03G0069500 Rorug03G0069600
rosa_samantha	Rh3AG126300 Rh3BG130300 Rh3CG132900 Rh3DG131700
rosa_wichuraiana	Rw3G010670

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
Acc16I	TGCGCA	1 cut(s)	689
AccB1I	GGYRCC	2 cut(s)	875, 1372
AccB7I	CCANNNNNTGG	1 cut(s)	1153
AccIII	TCCGGA	1 cut(s)	1194
AclI	AACGTT	1 cut(s)	138
AcoI	YGGCCR	1 cut(s)	147
AcsI	RAATTY	7 cut(s)	1221, 1421, 1481, 1556, 1697, 1847, 2137
AcuI	CTGAAG	7 cut(s)	275, 346, 1697, 2024, 2045, 2183, 2225
AcyI	GRCGYC	1 cut(s)	81
AfaI	GTAC	5 cut(s)	182, 698, 814, 834, 2274
AfiI	CCNNNNNNNGG	3 cut(s)	159, 1073, 1153
AflIII	ACRYGT	2 cut(s)	442, 1614
AjnI	CCWGG	3 cut(s)	185, 399, 745
AjuI	GAANNNNNNNTTGG	6 cut(s)	104, 128, 136, 160, 1206, 1238
Alw21I	GWGCWC	3 cut(s)	71, 228, 1146
Alw26I	GTCTC	2 cut(s)	847, 1379
AlwNI	CAGNNNCTG	1 cut(s)	2123
Aor13HI	TCCGGA	1 cut(s)	1194
AoxI	GGCC	5 cut(s)	147, 188, 470, 781, 2166
ApeKI	GCWGC	8 cut(s)	956, 995, 1267, 1570, 1667, 2066, 2117, 2120
ApoI	RAATTY	7 cut(s)	1221, 1421, 1481, 1556, 1697, 1847, 2137
AseI	ATTAAT	1 cut(s)	1965
Asp700I	GAANNNNTTC	1 cut(s)	1221
AspLEI	GCGC	2 cut(s)	690, 1079
AspS9I	GGNCC	3 cut(s)	529, 781, 1098
AsuHPI	GGTGA	5 cut(s)	178, 599, 701, 800, 1319
AvaII	GGWCC	2 cut(s)	529, 1098
AxyI	CCTNAGG	2 cut(s)	192, 1805
BaeGI	GKGCMC	1 cut(s)	880
BamHI	GGATCC	2 cut(s)	196, 1087
BanI	GGYRCC	2 cut(s)	875, 1372
BanII	GRGCYC	3 cut(s)	71, 1146, 1905
BarI	GAAGNNNNNNTAC	2 cut(s)	164, 196
BauI	CACGAG	1 cut(s)	176
BbsI	GAAGAC	3 cut(s)	1289, 1599, 2226
Bbv12I	GWGCWC	3 cut(s)	71, 228, 1146
BbvI	GCAGC	8 cut(s)	968, 982, 1279, 1557, 1654, 2078, 2129, 2132
BccI	CCATC	3 cut(s)	331, 575, 1043
BceAI	ACGGC	1 cut(s)	268
BciT130I	CCWGG	3 cut(s)	187, 401, 747
BciVI	GTATCC	1 cut(s)	1408
BclI	TGATCA	2 cut(s)	367, 1986
BcoDI	GTCTC	2 cut(s)	847, 1379
BfaI	CTAG	1 cut(s)	2244
BfmI	CTRYAG	2 cut(s)	576, 1227
BfuI	GTATCC	1 cut(s)	1408
BlpI	GCTNAGC	1 cut(s)	2113
Bme1390I	CCNGG	3 cut(s)	187, 401, 747
Bme18I	GGWCC	2 cut(s)	529, 1098
BmgT120I	GGNCC	3 cut(s)	529, 781, 1098
BmiI	GGNNCC	6 cut(s)	198, 530, 877, 1089, 1211, 1374
BmrFI	CCNGG	3 cut(s)	187, 401, 747
BmrI	ACTGGG	2 cut(s)	360, 788
BmsI	GCATC	6 cut(s)	645, 1062, 1186, 1525, 2227, 2249
BmuI	ACTGGG	2 cut(s)	360, 788
BpiI	GAAGAC	3 cut(s)	1289, 1599, 2226
BpmI	CTGGAG	3 cut(s)	55, 404, 1859
Bpu10I	CCTNAGC	3 cut(s)	158, 1062, 1547
Bpu1102I	GCTNAGC	1 cut(s)	2113
BpuEI	CTTGAG	1 cut(s)	1847
Bsa29I	ATCGAT	1 cut(s)	738
BsaBI	GATNNNNATC	1 cut(s)	201
BsaHI	GRCGYC	1 cut(s)	81
BsaJI	CCNNGG	5 cut(s)	185, 712, 784, 1344, 1443
BsaWI	WCCGGW	3 cut(s)	675, 1194, 1288
BsaXI	ACNNNNNCTCC	4 cut(s)	58, 88, 2213, 2243
Bsc4I	CCNNNNNNNGG	3 cut(s)	159, 1073, 1153
Bse118I	RCCGGY	1 cut(s)	271
Bse1I	ACTGG	7 cut(s)	30, 72, 355, 387, 568, 783, 939
Bse21I	CCTNAGG	2 cut(s)	192, 1805
Bse8I	GATNNNNATC	1 cut(s)	201
BseAI	TCCGGA	1 cut(s)	1194
BseBI	CCWGG	3 cut(s)	187, 401, 747
BseCI	ATCGAT	1 cut(s)	738
BseDI	CCNNGG	5 cut(s)	185, 712, 784, 1344, 1443
BseGI	GGATG	7 cut(s)	460, 660, 1056, 1144, 1516, 1801, 1885
BseJI	GATNNNNATC	1 cut(s)	201
BseLI	CCNNNNNNNGG	3 cut(s)	159, 1073, 1153
BseNI	ACTGG	7 cut(s)	30, 72, 355, 387, 568, 783, 939
BseRI	GAGGAG	2 cut(s)	145, 654
BseSI	GKGCMC	1 cut(s)	880
BseXI	GCAGC	8 cut(s)	968, 982, 1279, 1557, 1654, 2078, 2129, 2132
BsgI	GTGCAG	1 cut(s)	975
BshFI	GGCC	5 cut(s)	149, 190, 472, 783, 2168
BshNI	GGYRCC	2 cut(s)	875, 1372
BshVI	ATCGAT	1 cut(s)	738
BsiHKAI	GWGCWC	3 cut(s)	71, 228, 1146
BsiSI	CCGG	4 cut(s)	272, 676, 1195, 1289
BslFI	GGGAC	1 cut(s)	542
BslI	CCNNNNNNNGG	3 cut(s)	159, 1073, 1153
BsmAI	GTCTC	2 cut(s)	847, 1379
BsmBI	CGTCTC	1 cut(s)	1379
BsmFI	GGGAC	1 cut(s)	542
BsnI	GGCC	5 cut(s)	149, 190, 472, 783, 2168
Bsp1286I	GDGCHC	5 cut(s)	71, 228, 880, 1146, 1905
Bsp13I	TCCGGA	1 cut(s)	1194
Bsp1720I	GCTNAGC	1 cut(s)	2113
Bsp19I	CCATGG	1 cut(s)	712
BspANI	GGCC	5 cut(s)	149, 190, 472, 783, 2168
BspDI	ATCGAT	1 cut(s)	738
BspEI	TCCGGA	1 cut(s)	1194
BspLI	GGNNCC	6 cut(s)	198, 530, 877, 1089, 1211, 1374
BspT107I	GGYRCC	2 cut(s)	875, 1372
BsrFI	RCCGGY	1 cut(s)	271
BsrI	ACTGG	7 cut(s)	30, 72, 355, 387, 568, 783, 939
BssAI	RCCGGY	1 cut(s)	271
BssECI	CCNNGG	5 cut(s)	185, 712, 784, 1344, 1443
BssNI	GRCGYC	1 cut(s)	81
BssSI	CACGAG	1 cut(s)	176
BssT1I	CCWWGG	4 cut(s)	712, 784, 1344, 1443
Bst2BI	CACGAG	1 cut(s)	176
Bst2UI	CCWGG	3 cut(s)	187, 401, 747
Bst4CI	ACNGT	2 cut(s)	671, 2185
Bst6I	CTCTTC	1 cut(s)	965
BstACI	GRCGYC	1 cut(s)	81
BstAPI	GCANNNNNTGC	1 cut(s)	1918
BstDSI	CCRYGG	1 cut(s)	712
BstEII	GGTNACC	3 cut(s)	274, 605, 671
BstF5I	GGATG	7 cut(s)	460, 660, 1056, 1144, 1516, 1801, 1885
BstHHI	GCGC	2 cut(s)	690, 1079
BstMAI	GTCTC	2 cut(s)	847, 1379
BstMWI	GCNNNNNNNGC	7 cut(s)	956, 1091, 1529, 1543, 1909, 1918, 2158
BstNI	CCWGG	3 cut(s)	187, 401, 747
BstNSI	RCATGY	3 cut(s)	954, 1085, 1618
BstPI	GGTNACC	3 cut(s)	274, 605, 671
BstSCI	CCNGG	3 cut(s)	185, 399, 745
BstSFI	CTRYAG	2 cut(s)	576, 1227
BstSLI	GKGCMC	1 cut(s)	880
BstV1I	GCAGC	8 cut(s)	968, 982, 1279, 1557, 1654, 2078, 2129, 2132
BstV2I	GAAGAC	3 cut(s)	1289, 1599, 2226
BstX2I	RGATCY	4 cut(s)	55, 196, 1087, 1231
BstXI	CCANNNNNNTGG	1 cut(s)	2186
BstYI	RGATCY	4 cut(s)	55, 196, 1087, 1231
Bsu15I	ATCGAT	1 cut(s)	738
Bsu36I	CCTNAGG	2 cut(s)	192, 1805
BsuI	GTATCC	1 cut(s)	1408
BsuRI	GGCC	5 cut(s)	149, 190, 472, 783, 2168
BsuTUI	ATCGAT	1 cut(s)	738
BtgI	CCRYGG	1 cut(s)	712
BtgZI	GCGATG	1 cut(s)	1163
BtsCI	GGATG	7 cut(s)	460, 660, 1056, 1144, 1516, 1801, 1885
BtsIMutI	CAGTG	5 cut(s)	23, 370, 380, 561, 1376
CaiI	CAGNNNCTG	1 cut(s)	2123
CfoI	GCGC	2 cut(s)	690, 1079
Cfr10I	RCCGGY	1 cut(s)	271
Cfr13I	GGNCC	3 cut(s)	529, 781, 1098
ClaI	ATCGAT	1 cut(s)	738
CseI	GACGC	1 cut(s)	70
Csp6I	GTAC	5 cut(s)	181, 697, 813, 833, 2273
CviQI	GTAC	5 cut(s)	181, 697, 813, 833, 2273
DraI	TTTAAA	2 cut(s)	408, 2142
EaeI	YGGCCR	1 cut(s)	147
Eam1104I	CTCTTC	1 cut(s)	965
EarI	CTCTTC	1 cut(s)	965
Ecl136II	GAGCTC	2 cut(s)	69, 1144
Eco130I	CCWWGG	4 cut(s)	712, 784, 1344, 1443
Eco24I	GRGCYC	3 cut(s)	71, 1146, 1905
Eco32I	GATATC	2 cut(s)	12, 307
Eco47I	GGWCC	2 cut(s)	529, 1098
Eco53kI	GAGCTC	2 cut(s)	69, 1144
Eco57I	CTGAAG	7 cut(s)	275, 346, 1697, 2024, 2045, 2183, 2225
Eco81I	CCTNAGG	2 cut(s)	192, 1805
Eco91I	GGTNACC	3 cut(s)	274, 605, 671
EcoICRI	GAGCTC	2 cut(s)	69, 1144
EcoO65I	GGTNACC	3 cut(s)	274, 605, 671
EcoRI	GAATTC	2 cut(s)	1221, 1556
EcoRII	CCWGG	3 cut(s)	185, 399, 745
EcoRV	GATATC	2 cut(s)	12, 307
EcoT14I	CCWWGG	4 cut(s)	712, 784, 1344, 1443
EcoT22I	ATGCAT	1 cut(s)	2242
EcoT38I	GRGCYC	3 cut(s)	71, 1146, 1905
ErhI	CCWWGG	4 cut(s)	712, 784, 1344, 1443
Esp3I	CGTCTC	1 cut(s)	1379
FalI	AAGNNNNNCTT	4 cut(s)	34, 66, 1521, 1553
FaqI	GGGAC	1 cut(s)	542
FauNDI	CATATG	2 cut(s)	754, 1639
FbaI	TGATCA	2 cut(s)	367, 1986
FokI	GGATG	7 cut(s)	447, 667, 1043, 1151, 1503, 1808, 1892
FriOI	GRGCYC	3 cut(s)	71, 1146, 1905
FspBI	CTAG	1 cut(s)	2244
FspI	TGCGCA	1 cut(s)	689
GlaI	GCGC	2 cut(s)	689, 1078
GsuI	CTGGAG	3 cut(s)	55, 404, 1859
HaeIII	GGCC	5 cut(s)	149, 190, 472, 783, 2168
HapII	CCGG	4 cut(s)	272, 676, 1195, 1289
HgaI	GACGC	1 cut(s)	70
HhaI	GCGC	2 cut(s)	690, 1079
Hin1I	GRCGYC	1 cut(s)	81
Hin6I	GCGC	2 cut(s)	688, 1077
HinP1I	GCGC	2 cut(s)	688, 1077
HincII	GTYRAC	1 cut(s)	1690
HindII	GTYRAC	1 cut(s)	1690
HindIII	AAGCTT	4 cut(s)	981, 1535, 1544, 2110
HinfI	GANTC	8 cut(s)	424, 430, 1285, 1349, 2060, 2079, 2129, 2222
HpaI	GTTAAC	1 cut(s)	1690
HpaII	CCGG	4 cut(s)	272, 676, 1195, 1289
HphI	GGTGA	5 cut(s)	178, 599, 701, 800, 1319
Hpy166II	GTNNAC	1 cut(s)	1690
Hpy188I	TCNGA	9 cut(s)	55, 66, 207, 429, 772, 828, 1429, 1927, 2205
Hpy188III	TCNNGA	7 cut(s)	160, 200, 944, 1195, 1336, 1718, 2244
Hpy8I	GTNNAC	1 cut(s)	1690
Hpy99I	CGWCG	1 cut(s)	86
HpyCH4III	ACNGT	2 cut(s)	671, 2185
HpyCH4IV	ACGT	3 cut(s)	138, 442, 2267
HpyF10VI	GCNNNNNNNGC	7 cut(s)	956, 1091, 1529, 1543, 1909, 1918, 2158
HpySE526I	ACGT	3 cut(s)	138, 442, 2267
Hsp92I	GRCGYC	1 cut(s)	81
HspAI	GCGC	2 cut(s)	688, 1077
Kpn2I	TCCGGA	1 cut(s)	1194
Ksp22I	TGATCA	2 cut(s)	367, 1986
KspAI	GTTAAC	1 cut(s)	1690
LmnI	GCTCC	5 cut(s)	66, 74, 1149, 1585, 2092
Lsp1109I	GCAGC	8 cut(s)	968, 982, 1279, 1557, 1654, 2078, 2129, 2132
LweI	GCATC	6 cut(s)	645, 1062, 1186, 1525, 2227, 2249
MaeI	CTAG	1 cut(s)	2244
MaeII	ACGT	3 cut(s)	138, 442, 2267
MaeIII	GTNAC	6 cut(s)	274, 559, 605, 644, 671, 727
MfeI	CAATTG	1 cut(s)	723
MflI	RGATCY	4 cut(s)	55, 196, 1087, 1231
MhlI	GDGCHC	5 cut(s)	71, 228, 880, 1146, 1905
MlyI	GAGTC	3 cut(s)	424, 1279, 2216
MmeI	TCCRAC	3 cut(s)	1134, 2010, 2167
Mph1103I	ATGCAT	1 cut(s)	2242
MroI	TCCGGA	1 cut(s)	1194
MroXI	GAANNNNTTC	1 cut(s)	1221
MslI	CAYNNNNRTG	1 cut(s)	326
MspA1I	CMGCKG	2 cut(s)	253, 2123
MspI	CCGG	4 cut(s)	272, 676, 1195, 1289
MspR9I	CCNGG	3 cut(s)	187, 401, 747
MunI	CAATTG	1 cut(s)	723
MvaI	CCWGG	3 cut(s)	187, 401, 747
MwoI	GCNNNNNNNGC	7 cut(s)	956, 1091, 1529, 1543, 1909, 1918, 2158
NcoI	CCATGG	1 cut(s)	712
NdeI	CATATG	2 cut(s)	754, 1639
NlaIV	GGNNCC	6 cut(s)	198, 530, 877, 1089, 1211, 1374
NmeAIII	GCCGAG	2 cut(s)	1675, 2194
NmuCI	GTSAC	2 cut(s)	559, 605
NsbI	TGCGCA	1 cut(s)	689
NsiI	ATGCAT	1 cut(s)	2242
NspI	RCATGY	3 cut(s)	954, 1085, 1618
PciI	ACATGT	1 cut(s)	1614
PdmI	GAANNNNTTC	1 cut(s)	1221
PfeI	GAWTC	5 cut(s)	424, 1349, 2060, 2079, 2129
PflMI	CCANNNNNTGG	1 cut(s)	1153
PleI	GAGTC	3 cut(s)	424, 1279, 2216
PpsI	GAGTC	3 cut(s)	424, 1279, 2216
PscI	ACATGT	1 cut(s)	1614
PshBI	ATTAAT	1 cut(s)	1965
Psp124BI	GAGCTC	2 cut(s)	71, 1146
Psp1406I	AACGTT	1 cut(s)	138
Psp6I	CCWGG	3 cut(s)	185, 399, 745
PspEI	GGTNACC	3 cut(s)	274, 605, 671
PspGI	CCWGG	3 cut(s)	185, 399, 745
PspN4I	GGNNCC	6 cut(s)	198, 530, 877, 1089, 1211, 1374
PspPI	GGNCC	3 cut(s)	529, 781, 1098
PstNI	CAGNNNCTG	1 cut(s)	2123
PsuI	RGATCY	4 cut(s)	55, 196, 1087, 1231
PvuII	CAGCTG	1 cut(s)	2123
RsaI	GTAC	5 cut(s)	182, 698, 814, 834, 2274
RsaNI	GTAC	5 cut(s)	181, 697, 813, 833, 2273
RseI	CAYNNNNRTG	1 cut(s)	326
SacI	GAGCTC	2 cut(s)	71, 1146
Sau96I	GGNCC	3 cut(s)	529, 781, 1098
SchI	GAGTC	3 cut(s)	424, 1279, 2216
ScrFI	CCNGG	3 cut(s)	187, 401, 747
SduI	GDGCHC	5 cut(s)	71, 228, 880, 1146, 1905
SfaNI	GCATC	6 cut(s)	645, 1062, 1186, 1525, 2227, 2249
SfcI	CTRYAG	2 cut(s)	576, 1227
SinI	GGWCC	2 cut(s)	529, 1098
SmiMI	CAYNNNNRTG	1 cut(s)	326
SmlI	CTYRAG	1 cut(s)	1862
SmoI	CTYRAG	1 cut(s)	1862
SspI	AATATT	2 cut(s)	451, 637
SspMI	CTAG	1 cut(s)	2244
SstI	GAGCTC	2 cut(s)	71, 1146
StyD4I	CCNGG	3 cut(s)	185, 399, 745
StyI	CCWWGG	4 cut(s)	712, 784, 1344, 1443
TaaI	ACNGT	2 cut(s)	671, 2185
TaiI	ACGT	3 cut(s)	141, 445, 2270
TaqI	TCGA	6 cut(s)	8, 309, 738, 1611, 1944, 2192
TaqII	GACCGA	1 cut(s)	546
TatI	WGTACW	1 cut(s)	696
TauI	GCSGC	5 cut(s)	152, 253, 298, 1069, 1077
TfiI	GAWTC	5 cut(s)	424, 1349, 2060, 2079, 2129
TscAI	CASTG	5 cut(s)	30, 370, 387, 568, 1383
TseFI	GTSAC	2 cut(s)	559, 605
TseI	GCWGC	8 cut(s)	956, 995, 1267, 1570, 1667, 2066, 2117, 2120
Tsp45I	GTSAC	2 cut(s)	559, 605
TspDTI	ATGAA	8 cut(s)	512, 589, 724, 1202, 1268, 1605, 2211, 2232
TspGWI	ACGGA	1 cut(s)	84
TspRI	CASTG	5 cut(s)	30, 370, 387, 568, 1383
Van91I	CCANNNNNTGG	1 cut(s)	1153
VpaK11BI	GGWCC	2 cut(s)	529, 1098
VspI	ATTAAT	1 cut(s)	1965
XapI	RAATTY	7 cut(s)	1221, 1421, 1481, 1556, 1697, 1847, 2137
XbaI	TCTAGA	1 cut(s)	2243
XceI	RCATGY	3 cut(s)	954, 1085, 1618
XmnI	GAANNNNTTC	1 cut(s)	1221
XspI	CTAG	1 cut(s)	2244
Zsp2I	ATGCAT	1 cut(s)	2242

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.