Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr12

Orthogroup ID

OG0008815

Physical Location & Seq

Genomic Coordinates Forward (+)

23949353 .. 23954597

Gene Structure 5245 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

MD12G1159600.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 2286 bp

ATGGCTTCGATATCTCATTCTCCACTGGCGTTCGCCGGAACAAGCACCGCTTCGGATCTTTTCCGGAGCTCCAGTAATGGTGTCAGCGGTGTTCCGTTGAGAGCCCTAGGGAAGGTAAGGTTGGGCATGAGGAGGAACCTTGTGGTGGCCGCCAAGCTCAGGAAGGTTAAGAAGCATGAGTACCCCTGGCCCGAGGATCCGGATCCCAATGTCAAAGGTGGAGTGCTCACTCATCTCTCTCACTTCAAACCATTGAAGGAGAAGCCCAAGCCGGTTACTTTGCCGTTCGAAAAGCCGCTTGTTGATATCGAAAAGAAGATCATTGATGTGCGGAAGATGGCAAACGAAACTGGGCTGGACTTCAGTGATCAAATTATTTCATTGGAGAATAAATACAACCAGGCTTTAAAGGATTTATATACGAATCTCACTCCTATACAACGTGTGAATATTGCACGACATCCTAACAGGCCAACTTTCCTTGACCATGTGTTTAGCATTACTGATAAGTTTGTGGAGCTTCATGGAGACCGAGCAGGGTACGATGATCCTGCTATTGTCACTGGTATAGGAACAATAGATGGTAGAAGGTATATGTTCATGGGTCACCAGAAAGGTAGAAATACGAAGGAGAACATTATGCGTAACTTTGGGATGCCTACTCCTCACGGTTACCGGAAAGCTATGCGCATGATGTATTATGCAGATCACCATGGTTTCCCAATTATTACTTTCATCGATACCCCTGGTGCATATGCAGACCTTAAATCTGAGGAACTGGGCCAAGGTGAAGCCATAGCTCACAACTTGCGGACTATGTTTGGTCTGAAAGTACCTATTGTCTCTATTGTCATTGGAGAAGGTGGCTCTGGTGGTGCCCTTGCCATTGGCTGTGCTAATAAATTGTTAATGCTTGAAAATGCAGTCTTCTATGTTGCCAGTCCAGAAGCTTGTGCAGCAATCTTGTGGAAGAGTTCTAAAGCTTCTCCAAAGGCTGCTGAGAAGTTGAAAATTACTGCAACTGAGTTGTGCAAGCTGCAAATTGCAGATGGCATCATCCCTGAGCCGCTTGGTGGCGCACATGCGGATCCTGCTTGGACCTCTCAACAGATAAAAAATGCAATTAACAAAGAAATGGATGAGCTCCATAAGTTGGACACAGAAGCATTATTAAAGCATCGCATGATGAAGTTCCGGAAAATTGGAGGGTTCCAAGAAGGAATTCCTATAGATCCAAAGAAGAAAAAGAACATGAAACCAAAAGAAGAGCCTATCCATGTGAAGACTCCGGTTGCGGATTTGGAGGGTGAGGTTGAAAAGGTAAAACAGCAAATTTTGAAAGCCAAGGAATCTTCTAATGAGCCTTCATTGGTGCCACTGACTGAGACGATAGAAAAACTGAGAAAGGAGGTGGATGCTGAATTTTCTGAGGCAGTTAAAGCCTTGGGCTTGAAAGAGAGGTTTGCGACATTGCGAGAAGAATTTGCAAAAGTAAATGCACAGAACCAACTCCTGCATCCAGCTCTAAAAGCGAAGCTTGATAAGCTTAGGGAGGAATTCAACAAGGGGCTGCTCACAGCTCCTAATTATGAAGACCTAAAATATAAACTCGACATGTTGAAGGAATTATCTAAAGCATATGATCTTGCACAGAGCAACAAGAAGGCTGCAAAACTGAAGCAGGAAGTTAACCAGAAATTCAGCGAGATCATGGATCGGGAAGATGTTAAGGAGAAGGTTGTGGCACTTAGGGTTGAGGTTGAAAACTCTGGGGTGTCTAAATTTGATGATTTGGGTGACAGCCTGAAGGAGAAAATCGTGGAATTGAAGAAAGAGTTGGAAATTGAATTTATTGATGTCCTCAAGTCATTGGGTTTGGATGTTGAATTAAAAGCAAAAAAGCCAGTTGAGCAGACTTTGCCCTCAGAAATTAAGAACAAGATCGAAGATTTGAATGGAGAAATTAATGAGAGGATTGAAAGTGTAATCAATTCGACAGACCTGAAGGACAAGATTGAGCTACTGAAGTTGGAGATAGCAAAGGCAGGGAAAACACCTGATTCAGCAGCCAAAACTAGAATTGTGGCATTGGAGCAACAAATCAAACAAAGCTTAGCAGCAGCCGTGGAATCTTCAAATTTAAAAGAGAAGCATGAGAAGTTGAAGGCTGAGATTTCCCAAACCGTTGGATCGAATGGAAGTCTGAAGAAGGATGATGAAGATCCTTACTCCTTTGATGCATCTAGAGCAGTGGGTGCAAATCGCACCTTCGTACTTATCTTTTAG

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0009507 GO:0009526 GO:0009532 GO:0009536 GO:0009570 GO:0009941 GO:0016020 GO:0031967 GO:0031975 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044422 GO:0044424 GO:0044434 GO:0044435 GO:0044444 GO:0044446 GO:0044464

KEGG Pathways

Metabolic & Signaling

ko00061 ko00620 ko00640 ko00720 ko01100 ko01110 ko01120 ko01130 ko01200 ko01212 map00061 map00620 map00640 map00720 map01100 map01110 map01120 map01130 map01200 map01212

Pfam Domains

Protein Families

ACCA

Protein Analysis

762

Amino Acids

84.78

Weight (kDa)

8.9

Isoelectric Point (pI)

34.99

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
ACCA	PF03255	94 - 402	6e-132	Acetyl co-enzyme A carboxylase carboxyltransferase-like
Carboxyl_trans	PF01039	171 - 333	8.2e-08	Carboxyl transferase domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0008815)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT2G38040 AT2G38040
fragaria_vesca	FvH4_6g12150 FvH4_6g12150 FvH4_6g12150 FvH4_6g12150
malus_domestica	MD04G1146400.v1.1 MD12G1159600.v1.1
prunus_persica	Prupe.6G270200_v2.0.a1
pyrus_communis	pycom04g13200 pycom12g15130
rosa_chinensis	RchiOBHm_Chr3g0463941
rosa_laevigata	RLG00000024733
rosa_multiflora	Rmu_co8513843.1_g000002 Rmu_sc0006492.1_g000005
rosa_roxburghii	Rroxscaffold_6G00416120
rosa_rugosa	Rorug03G0069500 Rorug03G0069600
rosa_samantha	Rh3AG126300 Rh3BG130300 Rh3CG132900 Rh3DG131700
rosa_wichuraiana	Rw3G010670

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
Acc16I	TGCGCA	1 cut(s)	689
AccB1I	GGYRCC	2 cut(s)	875, 1372
AccB7I	CCANNNNNTGG	1 cut(s)	1153
AccIII	TCCGGA	3 cut(s)	63, 199, 1194
AciI	CCGC	9 cut(s)	48, 87, 150, 296, 331, 811, 1067, 1085, 1295
AcoI	YGGCCR	1 cut(s)	147
AcsI	RAATTY	9 cut(s)	1221, 1332, 1421, 1481, 1556, 1697, 1781, 1847, 2137
AcuI	CTGAAG	6 cut(s)	346, 1697, 1826, 2024, 2045, 2225
AfaI	GTAC	4 cut(s)	182, 542, 834, 2274
AfiI	CCNNNNNNNGG	5 cut(s)	112, 145, 159, 1073, 1153
AflIII	ACRYGT	2 cut(s)	442, 1614
AjnI	CCWGG	3 cut(s)	185, 399, 745
AjuI	GAANNNNNNNTTGG	6 cut(s)	104, 136, 1206, 1238, 1820, 1852
Alw21I	GWGCWC	3 cut(s)	71, 228, 1146
Alw26I	GTCTC	3 cut(s)	522, 847, 1379
Ama87I	CYCGRG	1 cut(s)	191
Aor13HI	TCCGGA	3 cut(s)	63, 199, 1194
AoxI	GGCC	4 cut(s)	147, 188, 470, 781
ApeKI	GCWGC	8 cut(s)	956, 995, 1036, 1570, 1667, 2066, 2117, 2120
ApoI	RAATTY	9 cut(s)	1221, 1332, 1421, 1481, 1556, 1697, 1781, 1847, 2137
AseI	ATTAAT	1 cut(s)	1965
Asp700I	GAANNNNTTC	1 cut(s)	1221
AspA2I	CCTAGG	1 cut(s)	106
AspLEI	GCGC	2 cut(s)	690, 1079
AspS9I	GGNCC	3 cut(s)	189, 781, 1098
AsuHPI	GGTGA	5 cut(s)	599, 701, 800, 1319, 1808
AsuII	TTCGAA	1 cut(s)	288
AvaI	CYCGRG	1 cut(s)	191
AvaII	GGWCC	1 cut(s)	1098
AvrII	CCTAGG	1 cut(s)	106
BaeGI	GKGCMC	1 cut(s)	880
BamHI	GGATCC	3 cut(s)	196, 202, 1087
BanI	GGYRCC	2 cut(s)	875, 1372
BanII	GRGCYC	3 cut(s)	71, 106, 1146
BarI	GAAGNNNNNNTAC	2 cut(s)	164, 196
BbsI	GAAGAC	3 cut(s)	919, 1289, 1599
Bbv12I	GWGCWC	3 cut(s)	71, 228, 1146
BbvI	GCAGC	8 cut(s)	968, 982, 1023, 1557, 1654, 2078, 2129, 2132
BccI	CCATC	3 cut(s)	331, 575, 1043
BceAI	ACGGC	2 cut(s)	268, 2108
BcgI	CGANNNNNNTGC	2 cut(s)	533, 567
BciT130I	CCWGG	3 cut(s)	187, 401, 747
BclI	TGATCA	1 cut(s)	367
BcoDI	GTCTC	3 cut(s)	522, 847, 1379
BfaI	CTAG	3 cut(s)	107, 2076, 2244
BfmI	CTRYAG	1 cut(s)	1227
BlnI	CCTAGG	1 cut(s)	106
BlpI	GCTNAGC	1 cut(s)	2113
Bme1390I	CCNGG	3 cut(s)	187, 401, 747
Bme18I	GGWCC	1 cut(s)	1098
BmeT110I	CYCGRG	1 cut(s)	191
BmgT120I	GGNCC	3 cut(s)	189, 781, 1098
BmiI	GGNNCC	7 cut(s)	137, 198, 204, 877, 1089, 1211, 1374
BmrFI	CCNGG	3 cut(s)	187, 401, 747
BmrI	ACTGGG	2 cut(s)	360, 788
BmsI	GCATC	7 cut(s)	645, 1062, 1186, 1405, 1525, 2227, 2249
BmuI	ACTGGG	2 cut(s)	360, 788
BpiI	GAAGAC	3 cut(s)	919, 1289, 1599
BpmI	CTGGAG	1 cut(s)	55
Bpu10I	CCTNAGC	3 cut(s)	158, 1062, 1547
Bpu1102I	GCTNAGC	1 cut(s)	2113
Bpu14I	TTCGAA	1 cut(s)	288
BpuEI	CTTGAG	1 cut(s)	1847
Bsa29I	ATCGAT	1 cut(s)	738
BsaBI	GATNNNNATC	2 cut(s)	201, 2220
BsaI	GGTCTC	1 cut(s)	522
BsaJI	CCNNGG	9 cut(s)	106, 185, 192, 712, 745, 784, 1344, 1443, 2124
BsaWI	WCCGGW	5 cut(s)	63, 199, 675, 1194, 1288
BsaXI	ACNNNNNCTCC	2 cut(s)	58, 88
Bsc4I	CCNNNNNNNGG	5 cut(s)	112, 145, 159, 1073, 1153
Bse118I	RCCGGY	1 cut(s)	271
Bse1I	ACTGG	7 cut(s)	30, 72, 355, 568, 783, 939, 1904
Bse3DI	GCAATG	1 cut(s)	1469
Bse8I	GATNNNNATC	2 cut(s)	201, 2220
BseAI	TCCGGA	3 cut(s)	63, 199, 1194
BseBI	CCWGG	3 cut(s)	187, 401, 747
BseCI	ATCGAT	1 cut(s)	738
BseDI	CCNNGG	9 cut(s)	106, 185, 192, 712, 745, 784, 1344, 1443, 2124
BseGI	GGATG	8 cut(s)	460, 660, 1056, 1144, 1420, 1516, 1885, 2218
BseJI	GATNNNNATC	2 cut(s)	201, 2220
BseLI	CCNNNNNNNGG	5 cut(s)	112, 145, 159, 1073, 1153
BseMI	GCAATG	1 cut(s)	1469
BseNI	ACTGG	7 cut(s)	30, 72, 355, 568, 783, 939, 1904
BseRI	GAGGAG	2 cut(s)	145, 654
BseSI	GKGCMC	1 cut(s)	880
BseXI	GCAGC	8 cut(s)	968, 982, 1023, 1557, 1654, 2078, 2129, 2132
BsgI	GTGCAG	1 cut(s)	975
BshFI	GGCC	4 cut(s)	149, 190, 472, 783
BshNI	GGYRCC	2 cut(s)	875, 1372
BshVI	ATCGAT	1 cut(s)	738
BsiHKAI	GWGCWC	3 cut(s)	71, 228, 1146
BsiHKCI	CYCGRG	1 cut(s)	191
BsiSI	CCGG	7 cut(s)	36, 64, 200, 272, 676, 1195, 1289
BslI	CCNNNNNNNGG	5 cut(s)	112, 145, 159, 1073, 1153
BsmAI	GTCTC	3 cut(s)	522, 847, 1379
BsmBI	CGTCTC	1 cut(s)	1379
BsnI	GGCC	4 cut(s)	149, 190, 472, 783
Bso31I	GGTCTC	1 cut(s)	522
BsoBI	CYCGRG	1 cut(s)	191
Bsp119I	TTCGAA	1 cut(s)	288
Bsp1286I	GDGCHC	5 cut(s)	71, 106, 228, 880, 1146
Bsp13I	TCCGGA	3 cut(s)	63, 199, 1194
Bsp1720I	GCTNAGC	1 cut(s)	2113
Bsp19I	CCATGG	1 cut(s)	712
BspACI	CCGC	9 cut(s)	48, 87, 150, 296, 331, 811, 1067, 1085, 1295
BspANI	GGCC	4 cut(s)	149, 190, 472, 783
BspDI	ATCGAT	1 cut(s)	738
BspEI	TCCGGA	3 cut(s)	63, 199, 1194
BspLI	GGNNCC	7 cut(s)	137, 198, 204, 877, 1089, 1211, 1374
BspQI	GCTCTTC	1 cut(s)	1260
BspT104I	TTCGAA	1 cut(s)	288
BspT107I	GGYRCC	2 cut(s)	875, 1372
BspTNI	GGTCTC	1 cut(s)	522
BsrDI	GCAATG	1 cut(s)	1469
BsrFI	RCCGGY	1 cut(s)	271
BsrI	ACTGG	7 cut(s)	30, 72, 355, 568, 783, 939, 1904
BssAI	RCCGGY	1 cut(s)	271
BssECI	CCNNGG	9 cut(s)	106, 185, 192, 712, 745, 784, 1344, 1443, 2124
BssT1I	CCWWGG	5 cut(s)	106, 712, 784, 1344, 1443
Bst2UI	CCWGG	3 cut(s)	187, 401, 747
Bst4CI	ACNGT	2 cut(s)	671, 2185
Bst6I	CTCTTC	2 cut(s)	965, 1260
BstAPI	GCANNNNNTGC	2 cut(s)	1918, 2255
BstBI	TTCGAA	1 cut(s)	288
BstC8I	GCNNGC	1 cut(s)	1034
BstDSI	CCRYGG	2 cut(s)	712, 2124
BstEII	GGTNACC	2 cut(s)	605, 671
BstENI	CCTNNNNNAGG	1 cut(s)	110
BstF5I	GGATG	8 cut(s)	460, 660, 1056, 1144, 1420, 1516, 1885, 2218
BstHHI	GCGC	2 cut(s)	690, 1079
BstMAI	GTCTC	3 cut(s)	522, 847, 1379
BstMWI	GCNNNNNNNGC	9 cut(s)	956, 1091, 1439, 1529, 1543, 1909, 1918, 2246, 2255
BstNI	CCWGG	3 cut(s)	187, 401, 747
BstNSI	RCATGY	2 cut(s)	1085, 1618
BstPI	GGTNACC	2 cut(s)	605, 671
BstSCI	CCNGG	3 cut(s)	185, 399, 745
BstSFI	CTRYAG	1 cut(s)	1227
BstSLI	GKGCMC	1 cut(s)	880
BstV1I	GCAGC	8 cut(s)	968, 982, 1023, 1557, 1654, 2078, 2129, 2132
BstV2I	GAAGAC	3 cut(s)	919, 1289, 1599
BstX2I	RGATCY	6 cut(s)	55, 196, 202, 1087, 1231, 2221
BstXI	CCANNNNNNTGG	1 cut(s)	2186
BstYI	RGATCY	6 cut(s)	55, 196, 202, 1087, 1231, 2221
Bsu15I	ATCGAT	1 cut(s)	738
BsuRI	GGCC	4 cut(s)	149, 190, 472, 783
BsuTUI	ATCGAT	1 cut(s)	738
BtgI	CCRYGG	2 cut(s)	712, 2124
BtgZI	GCGATG	1 cut(s)	1163
BtsCI	GGATG	8 cut(s)	460, 660, 1056, 1144, 1420, 1516, 1885, 2218
BtsI	GCAGTG	1 cut(s)	2256
BtsIMutI	CAGTG	5 cut(s)	23, 370, 561, 1376, 2256
Cac8I	GCNNGC	1 cut(s)	1034
CfoI	GCGC	2 cut(s)	690, 1079
Cfr10I	RCCGGY	1 cut(s)	271
Cfr13I	GGNCC	3 cut(s)	189, 781, 1098
ClaI	ATCGAT	1 cut(s)	738
Csp6I	GTAC	4 cut(s)	181, 541, 833, 2273
CviQI	GTAC	4 cut(s)	181, 541, 833, 2273
DraI	TTTAAA	2 cut(s)	408, 2142
EaeI	YGGCCR	1 cut(s)	147
Eam1104I	CTCTTC	2 cut(s)	965, 1260
EarI	CTCTTC	2 cut(s)	965, 1260
Ecl136II	GAGCTC	2 cut(s)	69, 1144
Eco130I	CCWWGG	5 cut(s)	106, 712, 784, 1344, 1443
Eco24I	GRGCYC	3 cut(s)	71, 106, 1146
Eco31I	GGTCTC	1 cut(s)	522
Eco32I	GATATC	2 cut(s)	12, 307
Eco47I	GGWCC	1 cut(s)	1098
Eco53kI	GAGCTC	2 cut(s)	69, 1144
Eco57I	CTGAAG	6 cut(s)	346, 1697, 1826, 2024, 2045, 2225
Eco88I	CYCGRG	1 cut(s)	191
Eco91I	GGTNACC	2 cut(s)	605, 671
EcoICRI	GAGCTC	2 cut(s)	69, 1144
EcoNI	CCTNNNNNAGG	1 cut(s)	110
EcoO65I	GGTNACC	2 cut(s)	605, 671
EcoRI	GAATTC	2 cut(s)	1221, 1556
EcoRII	CCWGG	3 cut(s)	185, 399, 745
EcoRV	GATATC	2 cut(s)	12, 307
EcoT14I	CCWWGG	5 cut(s)	106, 712, 784, 1344, 1443
EcoT22I	ATGCAT	1 cut(s)	2242
EcoT38I	GRGCYC	3 cut(s)	71, 106, 1146
ErhI	CCWWGG	5 cut(s)	106, 712, 784, 1344, 1443
Esp3I	CGTCTC	1 cut(s)	1379
FalI	AAGNNNNNCTT	4 cut(s)	34, 66, 1521, 1553
FauNDI	CATATG	2 cut(s)	754, 1639
FbaI	TGATCA	1 cut(s)	367
FokI	GGATG	8 cut(s)	447, 667, 1043, 1151, 1427, 1503, 1892, 2225
FriOI	GRGCYC	3 cut(s)	71, 106, 1146
FspAI	RTGCGCAY	1 cut(s)	689
FspBI	CTAG	3 cut(s)	107, 2076, 2244
FspI	TGCGCA	1 cut(s)	689
GlaI	GCGC	2 cut(s)	689, 1078
GsuI	CTGGAG	1 cut(s)	55
HaeIII	GGCC	4 cut(s)	149, 190, 472, 783
HapII	CCGG	7 cut(s)	36, 64, 200, 272, 676, 1195, 1289
HhaI	GCGC	2 cut(s)	690, 1079
Hin6I	GCGC	2 cut(s)	688, 1077
HinP1I	GCGC	2 cut(s)	688, 1077
HincII	GTYRAC	1 cut(s)	1690
HindII	GTYRAC	1 cut(s)	1690
HindIII	AAGCTT	5 cut(s)	948, 981, 1535, 1544, 2110
HinfI	GANTC	5 cut(s)	424, 1285, 1349, 2060, 2129
HpaI	GTTAAC	1 cut(s)	1690
HpaII	CCGG	7 cut(s)	36, 64, 200, 272, 676, 1195, 1289
HphI	GGTGA	5 cut(s)	599, 701, 800, 1319, 1808
Hpy166II	GTNNAC	1 cut(s)	1690
Hpy188I	TCNGA	6 cut(s)	55, 772, 828, 1429, 1927, 2205
Hpy188III	TCNNGA	7 cut(s)	64, 160, 200, 944, 1195, 1718, 2244
Hpy8I	GTNNAC	1 cut(s)	1690
HpyCH4III	ACNGT	2 cut(s)	671, 2185
HpyCH4IV	ACGT	1 cut(s)	442
HpyF10VI	GCNNNNNNNGC	9 cut(s)	956, 1091, 1439, 1529, 1543, 1909, 1918, 2246, 2255
HpySE526I	ACGT	1 cut(s)	442
HspAI	GCGC	2 cut(s)	688, 1077
Kpn2I	TCCGGA	3 cut(s)	63, 199, 1194
Ksp22I	TGATCA	1 cut(s)	367
KspAI	GTTAAC	1 cut(s)	1690
LguI	GCTCTTC	1 cut(s)	1260
LmnI	GCTCC	6 cut(s)	66, 74, 517, 1149, 1585, 2092
Lsp1109I	GCAGC	8 cut(s)	968, 982, 1023, 1557, 1654, 2078, 2129, 2132
LweI	GCATC	7 cut(s)	645, 1062, 1186, 1405, 1525, 2227, 2249
MaeI	CTAG	3 cut(s)	107, 2076, 2244
MaeII	ACGT	1 cut(s)	442
MaeIII	GTNAC	6 cut(s)	274, 559, 605, 644, 671, 1796
MflI	RGATCY	6 cut(s)	55, 196, 202, 1087, 1231, 2221
MhlI	GDGCHC	5 cut(s)	71, 106, 228, 880, 1146
MlyI	GAGTC	1 cut(s)	1279
MmeI	TCCRAC	4 cut(s)	1134, 1818, 2010, 2167
Mph1103I	ATGCAT	1 cut(s)	2242
MroI	TCCGGA	3 cut(s)	63, 199, 1194
MroXI	GAANNNNTTC	1 cut(s)	1221
MslI	CAYNNNNRTG	1 cut(s)	326
MspA1I	CMGCKG	1 cut(s)	87
MspI	CCGG	7 cut(s)	36, 64, 200, 272, 676, 1195, 1289
MspR9I	CCNGG	3 cut(s)	187, 401, 747
MvaI	CCWGG	3 cut(s)	187, 401, 747
MwoI	GCNNNNNNNGC	9 cut(s)	956, 1091, 1439, 1529, 1543, 1909, 1918, 2246, 2255
NcoI	CCATGG	1 cut(s)	712
NdeI	CATATG	2 cut(s)	754, 1639
NlaIV	GGNNCC	7 cut(s)	137, 198, 204, 877, 1089, 1211, 1374
NmuCI	GTSAC	3 cut(s)	559, 605, 1796
NsbI	TGCGCA	1 cut(s)	689
NsiI	ATGCAT	1 cut(s)	2242
NspI	RCATGY	2 cut(s)	1085, 1618
NspV	TTCGAA	1 cut(s)	288
PciI	ACATGT	1 cut(s)	1614
PciSI	GCTCTTC	1 cut(s)	1260
PdmI	GAANNNNTTC	1 cut(s)	1221
PfeI	GAWTC	4 cut(s)	424, 1349, 2060, 2129
PflMI	CCANNNNNTGG	1 cut(s)	1153
PleI	GAGTC	1 cut(s)	1279
PpsI	GAGTC	1 cut(s)	1279
PscI	ACATGT	1 cut(s)	1614
PshBI	ATTAAT	1 cut(s)	1965
Psp124BI	GAGCTC	2 cut(s)	71, 1146
Psp6I	CCWGG	3 cut(s)	185, 399, 745
PspEI	GGTNACC	2 cut(s)	605, 671
PspGI	CCWGG	3 cut(s)	185, 399, 745
PspN4I	GGNNCC	7 cut(s)	137, 198, 204, 877, 1089, 1211, 1374
PspPI	GGNCC	3 cut(s)	189, 781, 1098
PsuI	RGATCY	6 cut(s)	55, 196, 202, 1087, 1231, 2221
RsaI	GTAC	4 cut(s)	182, 542, 834, 2274
RsaNI	GTAC	4 cut(s)	181, 541, 833, 2273
RseI	CAYNNNNRTG	1 cut(s)	326
SacI	GAGCTC	2 cut(s)	71, 1146
SapI	GCTCTTC	1 cut(s)	1260
Sau96I	GGNCC	3 cut(s)	189, 781, 1098
SchI	GAGTC	1 cut(s)	1279
ScrFI	CCNGG	3 cut(s)	187, 401, 747
SduI	GDGCHC	5 cut(s)	71, 106, 228, 880, 1146
SfaNI	GCATC	7 cut(s)	645, 1062, 1186, 1405, 1525, 2227, 2249
SfcI	CTRYAG	1 cut(s)	1227
SfuI	TTCGAA	1 cut(s)	288
SinI	GGWCC	1 cut(s)	1098
SmiMI	CAYNNNNRTG	1 cut(s)	326
SmlI	CTYRAG	1 cut(s)	1862
SmoI	CTYRAG	1 cut(s)	1862
SsiI	CCGC	9 cut(s)	48, 87, 150, 296, 331, 811, 1067, 1085, 1295
SspI	AATATT	1 cut(s)	451
SspMI	CTAG	3 cut(s)	107, 2076, 2244
SstI	GAGCTC	2 cut(s)	71, 1146
StyD4I	CCNGG	3 cut(s)	185, 399, 745
StyI	CCWWGG	5 cut(s)	106, 712, 784, 1344, 1443
TaaI	ACNGT	2 cut(s)	671, 2185
TaiI	ACGT	1 cut(s)	445
TaqI	TCGA	8 cut(s)	8, 288, 309, 738, 1611, 1944, 1994, 2192
TaqII	GACCGA	1 cut(s)	546
TauI	GCSGC	3 cut(s)	152, 298, 1069
TfiI	GAWTC	4 cut(s)	424, 1349, 2060, 2129
TscAI	CASTG	5 cut(s)	30, 370, 568, 1383, 2256
TseFI	GTSAC	3 cut(s)	559, 605, 1796
TseI	GCWGC	8 cut(s)	956, 995, 1036, 1570, 1667, 2066, 2117, 2120
Tsp45I	GTSAC	3 cut(s)	559, 605, 1796
TspDTI	ATGAA	9 cut(s)	369, 512, 589, 724, 1202, 1268, 1356, 1605, 2232
TspGWI	ACGGA	1 cut(s)	84
TspRI	CASTG	5 cut(s)	30, 370, 568, 1383, 2256
Van91I	CCANNNNNTGG	1 cut(s)	1153
VpaK11BI	GGWCC	1 cut(s)	1098
VspI	ATTAAT	1 cut(s)	1965
XagI	CCTNNNNNAGG	1 cut(s)	110
XapI	RAATTY	9 cut(s)	1221, 1332, 1421, 1481, 1556, 1697, 1781, 1847, 2137
XbaI	TCTAGA	1 cut(s)	2243
XceI	RCATGY	2 cut(s)	1085, 1618
XmaJI	CCTAGG	1 cut(s)	106
XmnI	GAANNNNTTC	1 cut(s)	1221
XspI	CTAG	3 cut(s)	107, 2076, 2244
Zsp2I	ATGCAT	1 cut(s)	2242

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.