MD04G1161800.v1.1

helix loop helix domain

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr04
OG0014131
Physical Location & Seq
Reverse (-)
25280887 .. 25281480
594 bp
Loading structure...
UTR
Exon/CDS
Intron
MD04G1161800.v1.1.491

Sequence Viewer

Length: 594 bp
ATGGACTACCTCACTGCCACTGACTCTAACTCAAATAATTTCGCCAACTCTTCTTCCGTGACAAAAAACAATCGTGGTGGTGGGAAGAACAAAAGTAATGGAGTGAGGCTGTCCACTGACCCACAGAGCGTGGCGGCTAGGGAGAGGAGGCACAGGATCAGCGACCGCTTCAAGATCTTGCAGAGCTTGGTTCCTGGCGGGACGAAGCTGGACACGGTGTCCATGCTGGAGGAGGCTATCCACTATGTGAAGTTCCTCAAGACTCAGATATGGCTTCACCAGACCATGATCAACTTTGCGGAGGATTACCATGATCCTTCGTCGTCATCATCACCACCGTCCATGTTGATGGATATTCCAGCTCATGATCATGATCTTCATCCAATTGGGTGTCTTGATCATGATCTTCTTCCAACTGGGTGCTTTGATCATCTTTCTCACCAGCTTCATCAGCAGACTCATGATTTGTACGCTCACGGCGATGTCATTAGTACTGATGCTTCAGTGCAACCACCGTCACCACCAGCAGTTAATATTCCAATAATGCCCGAGTACAATTGCTTCCAAAGGTCAAGGAACAATTGGCAATATTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
HLH

Protein Analysis

198

Amino Acids

22.13

Weight (kDa)

6.19

Isoelectric Point (pI)

55.69

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
HLH PF00010 46 - 87 1.1e-09 Helix-loop-helix DNA-binding domain
bHLH_SAC51 PF23173 48 - 89 4.5e-09 Transcription factor SAC51 bHLH domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
Loading...
Style Settings
Image
Tree File
Tip: Beautify your tree with professional tools

Download the Full Tree (.nwk) file, then upload it to any of the following tools to customize colors, fonts, annotations, clades, and branch support.

Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Orthologous Genes (Group: OG0014131)

Species Orthologous Gene IDs
arabidopsis_thaliana AT5G01305
fragaria_vesca FvH4_6g11070
malus_domestica MD04G1161800.v1.1 MD12G1174500.v1.1
prunus_persica Prupe.6G281600_v2.0.a1
pyrus_communis pycom04g14470
rosa_chinensis RchiOBHm_Chr3g0462431
rosa_laevigata RLG00000024861
rosa_multiflora Rmu_co8365921.1_g000001 Rmu_sc0003850.1_g000005
rosa_roxburghii Rroxscaffold_6G00417520
rosa_rugosa Rorug03G0056500
rosa_samantha Rh3AG114900 Rh3BG118300 Rh3CG121100 Rh3DG120300
rosa_wichuraiana Rw3G009730

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AciI CCGC 4 cut(s) 134, 166, 198, 299
AclWI GGATC 2 cut(s) 164, 308
AcuI CTGAAG 1 cut(s) 486
AdeI CACNNNGTG 1 cut(s) 247
AfaI GTAC 3 cut(s) 470, 493, 554
AgsI TTSAA 1 cut(s) 172
AhdI GACNNNNNGTC 1 cut(s) 217
AjnI CCWGG 1 cut(s) 193
AluBI AGCT 4 cut(s) 186, 208, 362, 445
AluI AGCT 4 cut(s) 186, 208, 362, 445
AlwI GGATC 2 cut(s) 164, 308
Ama87I CYCGRG 1 cut(s) 548
ArsI GACNNNNNNTTYG 2 cut(s) 448, 480
AsuHPI GGTGA 4 cut(s) 269, 324, 431, 510
AvaI CYCGRG 1 cut(s) 548
BarI GAAGNNNNNNTAC 4 cut(s) 484, 516, 545, 577
BccI CCATC 1 cut(s) 343
BceAI ACGGC 1 cut(s) 493
BciT130I CCWGG 1 cut(s) 195
BclI TGATCA 4 cut(s) 288, 367, 397, 427
BfaI CTAG 1 cut(s) 138
BglII AGATCT 1 cut(s) 174
BisI GCNGC 1 cut(s) 135
BlsI GCNGC 1 cut(s) 136
BmcAI AGTACT 1 cut(s) 493
Bme1390I CCNGG 1 cut(s) 195
BmeRI GACNNNNNGTC 1 cut(s) 217
BmeT110I CYCGRG 1 cut(s) 548
BmiI GGNNCC 1 cut(s) 192
BmrFI CCNGG 1 cut(s) 195
BmrI ACTGGG 1 cut(s) 426
BmsI GCATC 1 cut(s) 487
BmuI ACTGGG 1 cut(s) 426
BpmI CTGGAG 1 cut(s) 248
BpuEI CTTGAG 1 cut(s) 242
BsaBI GATNNNNATC 3 cut(s) 372, 378, 402
Bse1I ACTGG 1 cut(s) 421
Bse8I GATNNNNATC 3 cut(s) 372, 378, 402
BseBI CCWGG 1 cut(s) 195
BseGI GGATG 1 cut(s) 379
BseJI GATNNNNATC 3 cut(s) 372, 378, 402
BseMII CTCAG 1 cut(s) 278
BseNI ACTGG 1 cut(s) 421
BseRI GAGGAG 2 cut(s) 160, 245
Bsh1285I CGRYCG 1 cut(s) 166
BsiEI CGRYCG 1 cut(s) 166
BsiHKCI CYCGRG 1 cut(s) 548
BslFI GGGAC 1 cut(s) 214
BsmFI GGGAC 1 cut(s) 214
BsoBI CYCGRG 1 cut(s) 548
Bsp143I GATC 9 cut(s) 156, 174, 288, 313, 367, 373, 397, 403, 427
BspACI CCGC 4 cut(s) 134, 166, 198, 299
BspCNI CTCAG 1 cut(s) 277
BspHI TCATGA 4 cut(s) 364, 370, 400, 460
BspLI GGNNCC 1 cut(s) 192
BspPI GGATC 2 cut(s) 164, 308
BsrI ACTGG 1 cut(s) 421
BssMI GATC 9 cut(s) 156, 174, 288, 313, 367, 373, 397, 403, 427
Bst2UI CCWGG 1 cut(s) 195
Bst4CI ACNGT 3 cut(s) 217, 339, 516
Bst6I CTCTTC 1 cut(s) 55
BstDEI CTNAG 1 cut(s) 264
BstF5I GGATG 1 cut(s) 379
BstKTI GATC 9 cut(s) 159, 177, 291, 316, 370, 376, 400, 406, 430
BstMBI GATC 9 cut(s) 156, 174, 288, 313, 367, 373, 397, 403, 427
BstMCI CGRYCG 1 cut(s) 166
BstMWI GCNNNNNNNGC 1 cut(s) 451
BstNI CCWGG 1 cut(s) 195
BstSCI CCNGG 1 cut(s) 193
BstX2I RGATCY 1 cut(s) 174
BstXI CCANNNNNNTGG 1 cut(s) 349
BstYI RGATCY 1 cut(s) 174
BtgZI GCGATG 1 cut(s) 495
BtsCI GGATG 1 cut(s) 379
BtsI GCAGTG 1 cut(s) 12
BtsIMutI CAGTG 4 cut(s) 12, 18, 114, 510
CciI TCATGA 4 cut(s) 364, 370, 400, 460
Csp6I GTAC 3 cut(s) 469, 492, 553
CspCI CAANNNNNGTGG 2 cut(s) 58, 93
CviAII CATG 8 cut(s) 223, 286, 311, 343, 365, 371, 401, 461
CviJI RGCY 8 cut(s) 109, 137, 186, 208, 236, 274, 362, 445
CviKI_1 RGCY 8 cut(s) 109, 137, 186, 208, 236, 274, 362, 445
CviQI GTAC 3 cut(s) 469, 492, 553
DdeI CTNAG 1 cut(s) 264
DpnI GATC 9 cut(s) 158, 176, 290, 315, 369, 375, 399, 405, 429
DpnII GATC 9 cut(s) 156, 174, 288, 313, 367, 373, 397, 403, 427
DraIII CACNNNGTG 1 cut(s) 247
DriI GACNNNNNGTC 1 cut(s) 217
Eam1104I CTCTTC 1 cut(s) 55
Eam1105I GACNNNNNGTC 1 cut(s) 217
EarI CTCTTC 1 cut(s) 55
Eco57I CTGAAG 1 cut(s) 486
Eco88I CYCGRG 1 cut(s) 548
EcoRII CCWGG 1 cut(s) 193
FaeI CATG 8 cut(s) 226, 289, 314, 346, 368, 374, 404, 464
FaqI GGGAC 1 cut(s) 214
FatI CATG 8 cut(s) 222, 285, 310, 342, 364, 370, 400, 460
FauI CCCGC 1 cut(s) 191
FbaI TGATCA 4 cut(s) 288, 367, 397, 427
Fnu4HI GCNGC 1 cut(s) 135
FokI GGATG 1 cut(s) 366
Fsp4HI GCNGC 1 cut(s) 135
FspBI CTAG 1 cut(s) 138
GluI GCNGC 1 cut(s) 135
GsuI CTGGAG 1 cut(s) 248
Hin1II CATG 8 cut(s) 226, 289, 314, 346, 368, 374, 404, 464
HinfI GANTC 3 cut(s) 23, 262, 457
HphI GGTGA 4 cut(s) 269, 324, 431, 510
Hpy166II GTNNAC 1 cut(s) 114
Hpy188I TCNGA 1 cut(s) 267
Hpy188III TCNNGA 7 cut(s) 172, 259, 365, 371, 395, 401, 461
Hpy8I GTNNAC 1 cut(s) 114
Hpy99I CGWCG 1 cut(s) 325
HpyAV CCTTC 1 cut(s) 327
HpyCH4III ACNGT 3 cut(s) 217, 339, 516
HpyCH4V TGCA 2 cut(s) 181, 508
HpyF10VI GCNNNNNNNGC 1 cut(s) 451
HpyF3I CTNAG 1 cut(s) 264
Hsp92II CATG 8 cut(s) 226, 289, 314, 346, 368, 374, 404, 464
Ksp22I TGATCA 4 cut(s) 288, 367, 397, 427
Kzo9I GATC 9 cut(s) 156, 174, 288, 313, 367, 373, 397, 403, 427
LweI GCATC 1 cut(s) 487
MaeI CTAG 1 cut(s) 138
MaeIII GTNAC 2 cut(s) 58, 516
MalI GATC 9 cut(s) 158, 176, 290, 315, 369, 375, 399, 405, 429
MboI GATC 9 cut(s) 156, 174, 288, 313, 367, 373, 397, 403, 427
MboII GAAGA 6 cut(s) 42, 45, 97, 368, 398, 401
MfeI CAATTG 3 cut(s) 384, 556, 580
MflI RGATCY 1 cut(s) 174
MluCI AATT 4 cut(s) 37, 384, 556, 580
MlyI GAGTC 3 cut(s) 17, 256, 451
MmeI TCCRAC 1 cut(s) 437
MnlI CCTC 8 cut(s) 20, 99, 138, 141, 223, 226, 266, 295
MseI TTAA 1 cut(s) 531
MslI CAYNNNNRTG 3 cut(s) 347, 369, 480
MspR9I CCNGG 1 cut(s) 195
MunI CAATTG 3 cut(s) 384, 556, 580
MvaI CCWGG 1 cut(s) 195
MwoI GCNNNNNNNGC 1 cut(s) 451
NdeII GATC 9 cut(s) 156, 174, 288, 313, 367, 373, 397, 403, 427
NlaIII CATG 8 cut(s) 226, 289, 314, 346, 368, 374, 404, 464
NlaIV GGNNCC 1 cut(s) 192
NmuCI GTSAC 2 cut(s) 58, 516
PagI TCATGA 4 cut(s) 364, 370, 400, 460
PcsI WCGNNNNNNNCGW 1 cut(s) 477
PkrI GCNGC 1 cut(s) 136
PleI GAGTC 3 cut(s) 17, 256, 451
PpsI GAGTC 3 cut(s) 17, 256, 451
Psp6I CCWGG 1 cut(s) 193
PspGI CCWGG 1 cut(s) 193
PspN4I GGNNCC 1 cut(s) 192
PsuI RGATCY 1 cut(s) 174
RsaI GTAC 3 cut(s) 470, 493, 554
RsaNI GTAC 3 cut(s) 469, 492, 553
RseI CAYNNNNRTG 3 cut(s) 347, 369, 480
SaqAI TTAA 1 cut(s) 531
SatI GCNGC 1 cut(s) 135
Sau3AI GATC 9 cut(s) 156, 174, 288, 313, 367, 373, 397, 403, 427
ScaI AGTACT 1 cut(s) 493
SchI GAGTC 3 cut(s) 17, 256, 451
ScrFI CCNGG 1 cut(s) 195
SetI ASST 6 cut(s) 12, 188, 210, 364, 447, 572
SfaNI GCATC 1 cut(s) 487
SmiMI CAYNNNNRTG 3 cut(s) 347, 369, 480
SmlI CTYRAG 1 cut(s) 257
SmoI CTYRAG 1 cut(s) 257
Sse9I AATT 4 cut(s) 37, 384, 556, 580
SsiI CCGC 4 cut(s) 134, 166, 198, 299
SspI AATATT 2 cut(s) 535, 590
SspMI CTAG 1 cut(s) 138
StyD4I CCNGG 1 cut(s) 193
TaaI ACNGT 3 cut(s) 217, 339, 516
TasI AATT 4 cut(s) 37, 384, 556, 580
TatI WGTACW 2 cut(s) 491, 552
TauI GCSGC 1 cut(s) 137
Tru1I TTAA 1 cut(s) 531
Tru9I TTAA 1 cut(s) 531
TscAI CASTG 4 cut(s) 19, 25, 121, 510
TseFI GTSAC 2 cut(s) 58, 516
Tsp45I GTSAC 2 cut(s) 58, 516
TspDTI ATGAA 2 cut(s) 368, 437
TspGWI ACGGA 1 cut(s) 46
TspRI CASTG 4 cut(s) 19, 25, 121, 510
XspI CTAG 1 cut(s) 138
ZrmI AGTACT 1 cut(s) 493
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.