Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr04

Orthogroup ID

OG0014411

Physical Location & Seq

Genomic Coordinates Reverse (-)

27530041 .. 27531804

Gene Structure 1764 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

MD04G1184300.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1764 bp

ATGTGCCCAAATTCAAAAACCAAAGCCATCCTATCCCTATTGCAAGGATGCAACGGCCTCAAACGGCTCAAAAAAATCCACGCCCACGTCATCACCATCGGTCTTCAACCCGATCCCGCCATTTCAAACAAGCTGCTCAACTTCTGCGCCGTCTCCGTCTCCGGCAGCTTGGCCTACGCGCAGCTGCTCTTCTACCACCATCTTCAAAACCCACAAACCCAGGATTGGAATTCCCTCATCAGAGGCTTCTCCAACAGCCCTTCTCCTCTCCACGCCATTTTTTACTACAATCTCATGCTTTCATCTGGTTCCGATTCTTCTCCTGATACTTTCACTTTCTCCTTCGTGCTCAAGGCCTGCGAGAAGTTGAAGGCCCGGAAGAAGTGCGAAGAGGTTCATGGAGCTGTGGTGAGGTTTGGGTACGAGAAGGACGTCGTTGTTTGCACCAATCTTATGAGATCCTATGCGGGAGATGGGTTGATTGAGAGTGCGGAGAAGGTTTTTGATAATATGCCTGAGAGAGACTTGGTGTCCTGGAATTCTATGATTTCGTGTTATTCTCAGAAGGGTCTTCACCATATGGCGTTGAAGACGTATAATCTTATGAGAAATGAGAATGTGGGTCTTGATGGGTTTACGATTGTTGGGTTGCTTTCGTCTTGTGCTCACTTGGGCGCATTGAATACCGGAGTTCAGATGCATAGAATTGCTAATGAAAAGGGGCTTGTGGGGAATGTTTATGTTGGGAATGCGCTTATTGATATGTATGCTAAATGTGGTAACTTGGATAGTGCTCTTTGCGTCTTTGAGAGAATGGGAAAGAAAGATGTTTTTACTTGGAACTCCATGATTGTTGGGTATGGAGTACATGGTCATGGAGAAGAAGCAATCTCATTTTTCAGGCGGATGTTGGTCGAAGGAGTCCAGCCAAACTCCATCACATTTCTTGGTTTGTTATGTGGATGCAGTCACCAAGGTTTGGTTGACAAGGGTGTCGAGTACTTCAATGTGATGAGCTCCAAGTTCAGTGTCAAGCCTGGAATTAAGCATTACGGGTGCCTGGTGGATCTGTTTGGCAGAGCTGGGATGCTCGAAAAGGCGCTTCAAGTTGTTGGGACTTCGCGTGCCCAGGAGGATCCAGTCCTTTGGCGGACTCTGCTTGGCTCTTGCAAGATTCATAAAAATGTAGAAATAGGAGAAATTGCCATGAGGAATCTAATCCAGCTAGGGTCAGCTAATGCAGGGGACTATGTGCTTCTTGCTTCGATTTATTCTACAGAGAAAAATGCAGATGGTGTTGTAAAGATGAGAAAATTGATCAAAACTGAGGGAGTAAAGACCACTTCTGGTTGGAGCTGGATTGAAATTGGTGATCAGGTTCACAAATTTGTCGTGGACGACAAGTCACATCCAGATGCTAATGAAATTTACCAAAAATTGAGGAAAATAGTTCATCGAGCTGCTCTGCATGGTTACGTGCAGGAGGATTCCCTCATGACAGTGTCTGAACTTACTTCCACAGACACAGATTGTTTGGGAACTTCCGGTTCATGTCACAGCGAGAAGCTGGCAATTGCTTTTGGCCTAGCAAGAACTCCAGAAGGAACGCCCTTACGAATAGTAAAGAACCTGAGAGTCTGTAGGGATTGTCATTCGTTTACAAAGTTTGTTTCGCAAGCTTTCGATCGGGAAATAATTGTTAGGGATCGTGTTCGGTTTCATCACTTCAAGGGTGGATTATGTTCATGTAAAGACTATTGGTGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0003676 GO:0003723 GO:0003824 GO:0004518 GO:0004519 GO:0005488 GO:0005575 GO:0005622 GO:0005623 GO:0006139 GO:0006725 GO:0006807 GO:0008150 GO:0008152 GO:0009451 GO:0009987 GO:0016070 GO:0016787 GO:0016788 GO:0034641 GO:0043170 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043412 GO:0044237 GO:0044238 GO:0044424 GO:0044464 GO:0046483 GO:0071704 GO:0090304 GO:0090305 GO:0097159 GO:1901360 GO:1901363

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

DYW_deaminase PPR PPR_2

Protein Analysis

588

Amino Acids

65.09

Weight (kDa)

8.74

Isoelectric Point (pI)

35.19

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
PPR_2	PF13041	175 - 223	1.9e-08	PPR repeat family
PPR	PF01535	177 - 207	3.5e-07	PPR repeat
PPR_2	PF13041	275 - 323	7e-12	PPR repeat family
PPR	PF01535	278 - 308	7.9e-08	PPR repeat
E_motif	PF20431	393 - 455	4e-14	E motif
Eplus_motif	PF20430	460 - 486	5.2e-09	E+ motif
DYW_deaminase	PF14432	518 - 587	6.2e-34	DYW family of nucleic acid deaminases

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0014411)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT3G56550 AT3G56550
fragaria_vesca	FvH4_6g08690
malus_domestica	MD04G1184300.v1.1 MD12G1197500.v1.1
prunus_persica	Prupe.6G302800_v2.0.a1
pyrus_communis	pycom04g16330 pycom12g18380
rosa_chinensis	RchiOBHm_Chr3g0458831
rosa_laevigata	RLG00000025132
rosa_multiflora	Rmu_co8385739.1_g000001
rosa_roxburghii	Rroxscaffold_6G00420760
rosa_rugosa	Rorug03G0032200
rosa_samantha	Rh3BG092700 Rh3CG093000 Rh3DG093700
rosa_wichuraiana	Rw3G007690

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AasI	GACNNNNNNGTC	1 cut(s)	992
AatII	GACGTC	1 cut(s)	435
AccB1I	GGYRCC	1 cut(s)	1056
AccB7I	CCANNNNNTGG	1 cut(s)	979
AccII	CGCG	2 cut(s)	179, 1123
AciI	CCGC	5 cut(s)	117, 467, 491, 904, 1150
AclWI	GGATC	6 cut(s)	107, 453, 1074, 1130, 1143, 1713
AcsI	RAATTY	5 cut(s)	10, 229, 538, 1385, 1425
AcyI	GRCGYC	1 cut(s)	432
AfaI	GTAC	3 cut(s)	422, 867, 1001
AfiI	CCNNNNNNNGG	2 cut(s)	225, 979
AhdI	GACNNNNNGTC	2 cut(s)	529, 1402
AjiI	CACGTC	1 cut(s)	88
AjnI	CCWGG	5 cut(s)	219, 533, 1036, 1059, 1128
Alw21I	GWGCWC	4 cut(s)	351, 667, 796, 1019
Alw26I	GTCTC	3 cut(s)	157, 163, 516
AlwI	GGATC	6 cut(s)	107, 453, 1074, 1130, 1143, 1713
AlwNI	CAGNNNCTG	1 cut(s)	1505
AoxI	GGCC	5 cut(s)	55, 171, 354, 372, 1582
ApeKI	GCWGC	5 cut(s)	133, 165, 181, 184, 1460
ApoI	RAATTY	5 cut(s)	10, 229, 538, 1385, 1425
Asp700I	GAANNNNTTC	1 cut(s)	393
AspLEI	GCGC	5 cut(s)	149, 181, 677, 754, 1102
AspS9I	GGNCC	1 cut(s)	373
AsuC2I	CCSGG	1 cut(s)	376
AsuHPI	GGTGA	5 cut(s)	85, 421, 566, 962, 1382
BaeGI	GKGCMC	2 cut(s)	8, 1129
BamHI	GGATCC	1 cut(s)	1135
BanI	GGYRCC	1 cut(s)	1056
BanII	GRGCYC	1 cut(s)	1019
BbsI	GAAGAC	3 cut(s)	95, 563, 596
Bbv12I	GWGCWC	4 cut(s)	351, 667, 796, 1019
BbvI	GCAGC	5 cut(s)	120, 171, 177, 193, 1447
BccI	CCATC	7 cut(s)	35, 104, 207, 467, 623, 944, 1286
BceAI	ACGGC	3 cut(s)	70, 80, 134
BciT130I	CCWGG	5 cut(s)	221, 535, 1038, 1061, 1130
BclI	TGATCA	2 cut(s)	1317, 1372
BcnI	CCSGG	1 cut(s)	376
BcoDI	GTCTC	3 cut(s)	157, 163, 516
BfaI	CTAG	2 cut(s)	1226, 1586
BfmI	CTRYAG	2 cut(s)	1275, 1639
BfoI	RGCGCY	1 cut(s)	1103
BisI	GCNGC	5 cut(s)	134, 166, 182, 185, 1461
BlsI	GCNGC	5 cut(s)	135, 167, 183, 186, 1462
BmcAI	AGTACT	1 cut(s)	1001
Bme1390I	CCNGG	6 cut(s)	221, 376, 535, 1038, 1061, 1130
BmeRI	GACNNNNNGTC	2 cut(s)	529, 1402
BmgBI	CACGTC	1 cut(s)	88
BmgT120I	GGNCC	1 cut(s)	373
BmiI	GGNNCC	3 cut(s)	310, 1058, 1137
BmrFI	CCNGG	6 cut(s)	221, 376, 535, 1038, 1061, 1130
BmsI	GCATC	5 cut(s)	38, 687, 953, 1077, 1405
BpiI	GAAGAC	3 cut(s)	95, 563, 596
BpmI	CTGGAG	1 cut(s)	1581
BpuEI	CTTGAG	1 cut(s)	335
BpuMI	CCSGG	1 cut(s)	376
BsaAI	YACGTR	1 cut(s)	1477
BsaHI	GRCGYC	1 cut(s)	432
BsaJI	CCNNGG	3 cut(s)	219, 973, 1128
BsaWI	WCCGGW	2 cut(s)	686, 1544
BsaXI	ACNNNNNCTCC	2 cut(s)	393, 423
Bsc4I	CCNNNNNNNGG	2 cut(s)	225, 979
Bse1I	ACTGG	1 cut(s)	1139
BseBI	CCWGG	5 cut(s)	221, 535, 1038, 1061, 1130
BseDI	CCNNGG	3 cut(s)	219, 973, 1128
BseGI	GGATG	6 cut(s)	27, 53, 912, 968, 1092, 1408
BseLI	CCNNNNNNNGG	2 cut(s)	225, 979
BseMII	CTCAG	4 cut(s)	507, 575, 1317, 1622
BseNI	ACTGG	1 cut(s)	1139
BseRI	GAGGAG	1 cut(s)	255
BseSI	GKGCMC	2 cut(s)	8, 1129
BseXI	GCAGC	5 cut(s)	120, 171, 177, 193, 1447
BseYI	CCCAGC	1 cut(s)	1082
BsgI	GTGCAG	1 cut(s)	1499
Bsh1236I	CGCG	2 cut(s)	179, 1123
Bsh1285I	CGRYCG	1 cut(s)	1687
BshFI	GGCC	5 cut(s)	57, 173, 356, 374, 1584
BshNI	GGYRCC	1 cut(s)	1056
BsiEI	CGRYCG	1 cut(s)	1687
BsiHKAI	GWGCWC	4 cut(s)	351, 667, 796, 1019
BsiSI	CCGG	4 cut(s)	162, 376, 687, 1545
BslFI	GGGAC	2 cut(s)	1129, 1259
BslI	CCNNNNNNNGG	2 cut(s)	225, 979
BsmAI	GTCTC	3 cut(s)	157, 163, 516
BsmBI	CGTCTC	2 cut(s)	157, 163
BsmFI	GGGAC	2 cut(s)	1129, 1259
BsmI	GAATGC	1 cut(s)	754
BsnI	GGCC	5 cut(s)	57, 173, 356, 374, 1584
Bsp1286I	GDGCHC	6 cut(s)	8, 351, 667, 796, 1019, 1129
Bsp143I	GATC	8 cut(s)	112, 458, 1066, 1135, 1317, 1372, 1684, 1705
BspACI	CCGC	5 cut(s)	117, 467, 491, 904, 1150
BspANI	GGCC	5 cut(s)	57, 173, 356, 374, 1584
BspCNI	CTCAG	4 cut(s)	508, 574, 1318, 1623
BspFNI	CGCG	2 cut(s)	179, 1123
BspHI	TCATGA	1 cut(s)	1494
BspLI	GGNNCC	3 cut(s)	310, 1058, 1137
BspPI	GGATC	6 cut(s)	107, 453, 1074, 1130, 1143, 1713
BspQI	GCTCTTC	1 cut(s)	194
BspT107I	GGYRCC	1 cut(s)	1056
BsrI	ACTGG	1 cut(s)	1139
BssECI	CCNNGG	3 cut(s)	219, 973, 1128
BssMI	GATC	8 cut(s)	112, 458, 1066, 1135, 1317, 1372, 1684, 1705
BssNI	GRCGYC	1 cut(s)	432
BssT1I	CCWWGG	1 cut(s)	973
Bst2UI	CCWGG	5 cut(s)	221, 535, 1038, 1061, 1130
Bst4CI	ACNGT	1 cut(s)	1501
Bst6I	CTCTTC	2 cut(s)	194, 384
BstACI	GRCGYC	1 cut(s)	432
BstBAI	YACGTR	1 cut(s)	1477
BstC8I	GCNNGC	4 cut(s)	358, 1125, 1569, 1677
BstDEI	CTNAG	4 cut(s)	516, 561, 1326, 1631
BstF5I	GGATG	6 cut(s)	27, 53, 912, 968, 1092, 1408
BstFNI	CGCG	2 cut(s)	179, 1123
BstH2I	RGCGCY	1 cut(s)	1103
BstHHI	GCGC	5 cut(s)	149, 181, 677, 754, 1102
BstKTI	GATC	8 cut(s)	115, 461, 1069, 1138, 1320, 1375, 1687, 1708
BstMAI	GTCTC	3 cut(s)	157, 163, 516
BstMBI	GATC	8 cut(s)	112, 458, 1066, 1135, 1317, 1372, 1684, 1705
BstMCI	CGRYCG	1 cut(s)	1687
BstMWI	GCNNNNNNNGC	1 cut(s)	1156
BstNI	CCWGG	5 cut(s)	221, 535, 1038, 1061, 1130
BstSCI	CCNGG	6 cut(s)	219, 374, 533, 1036, 1059, 1128
BstSFI	CTRYAG	2 cut(s)	1275, 1639
BstSLI	GKGCMC	2 cut(s)	8, 1129
BstUI	CGCG	2 cut(s)	179, 1123
BstV1I	GCAGC	5 cut(s)	120, 171, 177, 193, 1447
BstV2I	GAAGAC	3 cut(s)	95, 563, 596
BstX2I	RGATCY	3 cut(s)	458, 1066, 1135
BstXI	CCANNNNNNTGG	1 cut(s)	1146
BstYI	RGATCY	3 cut(s)	458, 1066, 1135
BsuRI	GGCC	5 cut(s)	57, 173, 356, 374, 1584
BtrI	CACGTC	1 cut(s)	88
BtsCI	GGATG	6 cut(s)	27, 53, 912, 968, 1092, 1408
BtsIMutI	CAGTG	2 cut(s)	1033, 1506
Cac8I	GCNNGC	4 cut(s)	358, 1125, 1569, 1677
CaiI	CAGNNNCTG	1 cut(s)	1505
CciI	TCATGA	1 cut(s)	1494
CfoI	GCGC	5 cut(s)	149, 181, 677, 754, 1102
Cfr13I	GGNCC	1 cut(s)	373
CseI	GACGC	1 cut(s)	790
Csp6I	GTAC	3 cut(s)	421, 866, 1000
CviQI	GTAC	3 cut(s)	421, 866, 1000
DdeI	CTNAG	4 cut(s)	516, 561, 1326, 1631
DpnI	GATC	8 cut(s)	114, 460, 1068, 1137, 1319, 1374, 1686, 1707
DpnII	GATC	8 cut(s)	112, 458, 1066, 1135, 1317, 1372, 1684, 1705
DrdI	GACNNNNNNGTC	1 cut(s)	992
DriI	GACNNNNNGTC	2 cut(s)	529, 1402
DseDI	GACNNNNNNGTC	1 cut(s)	992
Eam1104I	CTCTTC	2 cut(s)	194, 384
Eam1105I	GACNNNNNGTC	2 cut(s)	529, 1402
EarI	CTCTTC	2 cut(s)	194, 384
EciI	GGCGGA	2 cut(s)	919, 1165
Ecl136II	GAGCTC	1 cut(s)	1017
Eco130I	CCWWGG	1 cut(s)	973
Eco147I	AGGCCT	1 cut(s)	356
Eco24I	GRGCYC	1 cut(s)	1019
Eco53kI	GAGCTC	1 cut(s)	1017
EcoICRI	GAGCTC	1 cut(s)	1017
EcoRI	GAATTC	2 cut(s)	229, 538
EcoRII	CCWGG	5 cut(s)	219, 533, 1036, 1059, 1128
EcoT14I	CCWWGG	1 cut(s)	973
EcoT22I	ATGCAT	1 cut(s)	702
EcoT38I	GRGCYC	1 cut(s)	1019
ErhI	CCWWGG	1 cut(s)	973
Esp3I	CGTCTC	2 cut(s)	157, 163
FaqI	GGGAC	2 cut(s)	1129, 1259
FauI	CCCGC	2 cut(s)	124, 460
FauNDI	CATATG	1 cut(s)	579
FbaI	TGATCA	2 cut(s)	1317, 1372
Fnu4HI	GCNGC	5 cut(s)	134, 166, 182, 185, 1461
FokI	GGATG	6 cut(s)	14, 60, 919, 975, 1099, 1395
FriOI	GRGCYC	1 cut(s)	1019
Fsp4HI	GCNGC	5 cut(s)	134, 166, 182, 185, 1461
FspBI	CTAG	2 cut(s)	1226, 1586
GlaI	GCGC	5 cut(s)	148, 180, 676, 753, 1101
GluI	GCNGC	5 cut(s)	134, 166, 182, 185, 1461
GsaI	CCCAGC	1 cut(s)	1086
GsuI	CTGGAG	1 cut(s)	1581
HaeII	RGCGCY	1 cut(s)	1103
HaeIII	GGCC	5 cut(s)	57, 173, 356, 374, 1584
HapII	CCGG	4 cut(s)	162, 376, 687, 1545
HgaI	GACGC	1 cut(s)	790
HhaI	GCGC	5 cut(s)	149, 181, 677, 754, 1102
Hin1I	GRCGYC	1 cut(s)	432
Hin6I	GCGC	5 cut(s)	147, 179, 675, 752, 1100
HinP1I	GCGC	5 cut(s)	147, 179, 675, 752, 1100
HincII	GTYRAC	1 cut(s)	985
HindII	GTYRAC	1 cut(s)	985
HindIII	AAGCTT	1 cut(s)	1677
HinfI	GANTC	7 cut(s)	314, 921, 1153, 1174, 1213, 1487, 1635
HpaII	CCGG	4 cut(s)	162, 376, 687, 1545
HphI	GGTGA	5 cut(s)	85, 421, 566, 962, 1382
Hpy166II	GTNNAC	5 cut(s)	636, 985, 1381, 1396, 1659
Hpy188I	TCNGA	5 cut(s)	242, 313, 564, 696, 1507
Hpy188III	TCNNGA	6 cut(s)	323, 626, 1412, 1495, 1598, 1688
Hpy8I	GTNNAC	5 cut(s)	636, 985, 1381, 1396, 1659
Hpy99I	CGWCG	1 cut(s)	437
HpyAV	CCTTC	8 cut(s)	270, 352, 364, 421, 490, 559, 911, 1595
HpyCH4III	ACNGT	1 cut(s)	1501
HpyCH4IV	ACGT	4 cut(s)	87, 432, 593, 1476
HpyF10VI	GCNNNNNNNGC	1 cut(s)	1156
HpyF3I	CTNAG	4 cut(s)	516, 561, 1326, 1631
HpySE526I	ACGT	4 cut(s)	87, 432, 593, 1476
Hsp92I	GRCGYC	1 cut(s)	432
HspAI	GCGC	5 cut(s)	147, 179, 675, 752, 1100
Ksp22I	TGATCA	2 cut(s)	1317, 1372
Kzo9I	GATC	8 cut(s)	112, 458, 1066, 1135, 1317, 1372, 1684, 1705
LguI	GCTCTTC	1 cut(s)	194
LmnI	GCTCC	3 cut(s)	401, 1022, 1353
Lsp1109I	GCAGC	5 cut(s)	120, 171, 177, 193, 1447
LweI	GCATC	5 cut(s)	38, 687, 953, 1077, 1405
MaeI	CTAG	2 cut(s)	1226, 1586
MaeII	ACGT	4 cut(s)	87, 432, 593, 1476
MaeIII	GTNAC	5 cut(s)	779, 968, 1404, 1472, 1553
MalI	GATC	8 cut(s)	114, 460, 1068, 1137, 1319, 1374, 1686, 1707
MboI	GATC	8 cut(s)	112, 458, 1066, 1135, 1317, 1372, 1684, 1705
MboII	GAAGA	9 cut(s)	95, 181, 194, 309, 391, 401, 563, 601, 893
MfeI	CAATTG	1 cut(s)	1572
MflI	RGATCY	3 cut(s)	458, 1066, 1135
MhlI	GDGCHC	6 cut(s)	8, 351, 667, 796, 1019, 1129
MlyI	GAGTC	3 cut(s)	930, 1147, 1644
MmeI	TCCRAC	2 cut(s)	276, 1331
Mph1103I	ATGCAT	1 cut(s)	702
MroXI	GAANNNNTTC	1 cut(s)	393
MseI	TTAA	1 cut(s)	1044
MslI	CAYNNNNRTG	4 cut(s)	873, 1182, 1413, 1499
MspA1I	CMGCKG	1 cut(s)	184
MspI	CCGG	4 cut(s)	162, 376, 687, 1545
MspR9I	CCNGG	6 cut(s)	221, 376, 535, 1038, 1061, 1130
MunI	CAATTG	1 cut(s)	1572
Mva1269I	GAATGC	1 cut(s)	754
MvaI	CCWGG	5 cut(s)	221, 535, 1038, 1061, 1130
MvnI	CGCG	2 cut(s)	179, 1123
MwoI	GCNNNNNNNGC	1 cut(s)	1156
NciI	CCSGG	1 cut(s)	376
NdeI	CATATG	1 cut(s)	579
NdeII	GATC	8 cut(s)	112, 458, 1066, 1135, 1317, 1372, 1684, 1705
NlaIV	GGNNCC	3 cut(s)	310, 1058, 1137
NmuCI	GTSAC	3 cut(s)	968, 1404, 1553
NsiI	ATGCAT	1 cut(s)	702
PagI	TCATGA	1 cut(s)	1494
PceI	AGGCCT	1 cut(s)	356
PciSI	GCTCTTC	1 cut(s)	194
PcsI	WCGNNNNNNNCGW	1 cut(s)	429
PctI	GAATGC	1 cut(s)	754
PdmI	GAANNNNTTC	1 cut(s)	393
PfeI	GAWTC	4 cut(s)	314, 1174, 1213, 1487
PflFI	GACNNNGTC	1 cut(s)	1501
PflMI	CCANNNNNTGG	1 cut(s)	979
PfoI	TCCNGGA	1 cut(s)	533
PkrI	GCNGC	5 cut(s)	135, 167, 183, 186, 1462
Ple19I	CGATCG	1 cut(s)	1687
PleI	GAGTC	3 cut(s)	929, 1147, 1643
PpsI	GAGTC	3 cut(s)	929, 1147, 1643
Ppu21I	YACGTR	1 cut(s)	1477
Psp124BI	GAGCTC	1 cut(s)	1019
Psp6I	CCWGG	5 cut(s)	219, 533, 1036, 1059, 1128
PspFI	CCCAGC	1 cut(s)	1082
PspGI	CCWGG	5 cut(s)	219, 533, 1036, 1059, 1128
PspN4I	GGNNCC	3 cut(s)	310, 1058, 1137
PspPI	GGNCC	1 cut(s)	373
PstNI	CAGNNNCTG	1 cut(s)	1505
PsuI	RGATCY	3 cut(s)	458, 1066, 1135
PsyI	GACNNNGTC	1 cut(s)	1501
PvuI	CGATCG	1 cut(s)	1687
PvuII	CAGCTG	1 cut(s)	184
RsaI	GTAC	3 cut(s)	422, 867, 1001
RsaNI	GTAC	3 cut(s)	421, 866, 1000
RseI	CAYNNNNRTG	4 cut(s)	873, 1182, 1413, 1499
SacI	GAGCTC	1 cut(s)	1019
SapI	GCTCTTC	1 cut(s)	194
SaqAI	TTAA	1 cut(s)	1044
SatI	GCNGC	5 cut(s)	134, 166, 182, 185, 1461
Sau3AI	GATC	8 cut(s)	112, 458, 1066, 1135, 1317, 1372, 1684, 1705
Sau96I	GGNCC	1 cut(s)	373
ScaI	AGTACT	1 cut(s)	1001
SchI	GAGTC	3 cut(s)	930, 1147, 1644
ScrFI	CCNGG	6 cut(s)	221, 376, 535, 1038, 1061, 1130
SduI	GDGCHC	6 cut(s)	8, 351, 667, 796, 1019, 1129
SfaNI	GCATC	5 cut(s)	38, 687, 953, 1077, 1405
SfcI	CTRYAG	2 cut(s)	1275, 1639
SmiMI	CAYNNNNRTG	4 cut(s)	873, 1182, 1413, 1499
SmlI	CTYRAG	1 cut(s)	350
SmoI	CTYRAG	1 cut(s)	350
SseBI	AGGCCT	1 cut(s)	356
SsiI	CCGC	5 cut(s)	117, 467, 491, 904, 1150
SspMI	CTAG	2 cut(s)	1226, 1586
SstI	GAGCTC	1 cut(s)	1019
StuI	AGGCCT	1 cut(s)	356
StyD4I	CCNGG	6 cut(s)	219, 374, 533, 1036, 1059, 1128
StyI	CCWWGG	1 cut(s)	973
TaaI	ACNGT	1 cut(s)	1501
TaiI	ACGT	4 cut(s)	90, 435, 596, 1479
TaqI	TCGA	6 cut(s)	915, 996, 1092, 1265, 1456, 1683
TaqII	GACCGA	1 cut(s)	89
TatI	WGTACW	2 cut(s)	865, 999
TfiI	GAWTC	4 cut(s)	314, 1174, 1213, 1487
Tru1I	TTAA	1 cut(s)	1044
Tru9I	TTAA	1 cut(s)	1044
TscAI	CASTG	2 cut(s)	1033, 1506
TseFI	GTSAC	3 cut(s)	968, 1404, 1553
TseI	GCWGC	5 cut(s)	133, 165, 181, 184, 1460
Tsp45I	GTSAC	3 cut(s)	968, 1404, 1553
TspDTI	ATGAA	9 cut(s)	291, 386, 729, 1166, 1437, 1442, 1539, 1709, 1734
TspGWI	ACGGA	1 cut(s)	145
TspRI	CASTG	2 cut(s)	1033, 1506
Tth111I	GACNNNGTC	1 cut(s)	1501
Van91I	CCANNNNNTGG	1 cut(s)	979
XapI	RAATTY	5 cut(s)	10, 229, 538, 1385, 1425
XmnI	GAANNNNTTC	1 cut(s)	393
XspI	CTAG	2 cut(s)	1226, 1586
ZraI	GACGTC	1 cut(s)	433
ZrmI	AGTACT	1 cut(s)	1001
Zsp2I	ATGCAT	1 cut(s)	702

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.