Basic Information

Type: gene

Biological Identity

Species

rosa_wichuraiana

Chromosome

Chr3

Orthogroup ID

OG0014411

Physical Location & Seq

Genomic Coordinates Forward (+)

7100495 .. 7102501

Gene Structure 2007 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

Rw3G007690.1

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1785 bp

ATGTTGACAAACCATACCTTCCAAGCTTCGAAAACCAAAGCCATCCTAACCCTCTTGCAAGGATGCAACACCCTCAAAAGGCTCAGAAAAATCCACGCCCACGTCATCACCAACGGTCTTCAACACCACCCTTCCATTTGCAACAAGCTCCTCAACTTCTGCGCCGTCTCCGTCTCAGGCAGCTTACCCTACGCTCAGCTGCTCTTCCATTATCACATCCCAAACCCACAAACCCAAGACTGGAATTCCATGATCAGAGGCTTCTCCCAAAGCCCATCTCCTCTTCAAGCCATTTCTTACTACAACCACATGCTTTCGTCCTCCTCCGCTTCCGATTTCCCACCCGACACGTTTAGCTTCTCGTTTGTGCTCAAGGCTTGCGAGAGAGTCAGGGCAGAGAGCAAGTGCCGAGAGGTTCATGGAGTTGTGGTTCGCCGTGGTTATGATATGGACGTTGTTGTTTGCACCAATTTGATGAAGTCTTATGCTGGCAGTGGGTCAGTTGATAATGCACGGAGAGTGTTCGACGAAATGTCTGAGAGAGACTTGGTGTGTTGGAATTCCATGATCTCGTGCTACTCTCAAGCGGGTCTTCACCATGAGGCGTTGAATATATTTAGTCTTATGAGAAATCAAAATGTGGGTCTTGATGGGTTCACCCTCGTTGGGTTGCTTTCGTCTTGTGCTCACTTGGGTGCGTTGAACACCGGAGTCACAATGCATGAACTTGCTCGTCAGAAGGGGTTTTTGGGAAGTGTTTATGTTGGCAATGCGCTTATAGATATGTACGCAAAATGTGGTGACTTGGATGGTGCTCTTTGTGTCTTTGAGAGAATGAGAAAGCGGGATGCTTTTACGTGGAACTCCATGATTGTTGGGTATGGAGTACATGGTCGCGGGGACGAAGCAATCTATTTTTTTGAGAAGATGATGATGGCAGGTGTGCAGCCGAATTCCATCACATTTCTCGGTTTGTTATGTGGGTGTAGTCACCAAGGATTAGTAGAGAAGGGTGTTGATTACTTCCATATGATGAGCTCCAAGTTCAATATCAAACCTGGAATTAAGCACTATGGGTGTCTGGTGGATCTGTTTGGTCGAGCTGGGAACCTCGAGAAGGCGCTTCATGTTATTAGGGCATCACATGCACAGGATGATCCAGTTCTATGGCGGACTCTGCTCAGCTCTTGCAAGATTCATAAAAACGTGGAAATAGGAGAAGTTGCCTTGAAGAATCTAATCCGTCTAGAGTCATCGAATGCAGGGGACTACGTACTTCTTGCCACTATTTATTTTAGGGCGAAAGATGCAGATGGTGTTTCAAGGATGAGAAAATTGATTAAAAACCAGGGAGTGAGGACCACTCCTGGTTGGAGCTGGATTGAAGTTGGCGATCAGGTTCACAAATTTGTAGTGGATGACAAGTCACATCCAGATACTGAAGAAATCTATCGAAAATTGGAGGTAGTAGTCCAACGAGCTGCTCTACATGGTTATCTGCAAGAGGAATCTCTTATCAGAGCCTGTGAATTTACCTCCATAGACACCAACTGTTCGGGAACTTCAGGTTCATGTCACAGTGAGAAGCTGGCAATTGCTTTTGGCTTGGCAAGAACTCCAGAAGGAACATGTTTACGGATAGTAAAAAACCTGAGAGTTTGTAGAGATTGCCATTCATTTACAAAATTTGTTTCCCAAGCATTTAATCGCGAAATAATTGTTAGGGACCGAGTTCGGTTTCATCACTTCAAGGGTGGATTGTGTAGTTGTAAAGACTATTGGTGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0003676 GO:0003723 GO:0003824 GO:0004518 GO:0004519 GO:0005488 GO:0005575 GO:0005622 GO:0005623 GO:0006139 GO:0006725 GO:0006807 GO:0008150 GO:0008152 GO:0009451 GO:0009987 GO:0016070 GO:0016787 GO:0016788 GO:0034641 GO:0043170 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043412 GO:0044237 GO:0044238 GO:0044424 GO:0044464 GO:0046483 GO:0071704 GO:0090304 GO:0090305 GO:0097159 GO:1901360 GO:1901363

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

DYW_deaminase PPR PPR_2 PPR_3

Protein Analysis

594

Amino Acids

66.53

Weight (kDa)

8.81

Isoelectric Point (pI)

37.54

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
PPR_2	PF13041	182 - 230	1e-07	PPR repeat family
PPR	PF01535	184 - 213	1.1e-07	PPR repeat
PPR_2	PF13041	283 - 330	7.9e-11	PPR repeat family
PPR	PF01535	285 - 315	4.2e-07	PPR repeat
E_motif	PF20431	400 - 462	2.8e-15	E motif
Eplus_motif	PF20430	467 - 493	9.7e-07	E+ motif
DYW_deaminase	PF14432	525 - 594	1.9e-33	DYW family of nucleic acid deaminases

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0014411)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT3G56550 AT3G56550
fragaria_vesca	FvH4_6g08690
malus_domestica	MD04G1184300.v1.1 MD12G1197500.v1.1
prunus_persica	Prupe.6G302800_v2.0.a1
pyrus_communis	pycom04g16330 pycom12g18380
rosa_chinensis	RchiOBHm_Chr3g0458831
rosa_laevigata	RLG00000025132
rosa_multiflora	Rmu_co8385739.1_g000001
rosa_roxburghii	Rroxscaffold_6G00420760
rosa_rugosa	Rorug03G0032200
rosa_samantha	Rh3BG092700 Rh3CG093000 Rh3DG093700
rosa_wichuraiana	Rw3G007690

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AarI	CACCTGC	1 cut(s)	929
Acc36I	ACCTGC	1 cut(s)	929
AccII	CGCG	2 cut(s)	897, 1710
AciI	CCGC	5 cut(s)	327, 587, 844, 897, 1171
AclWI	GGATC	2 cut(s)	1095, 1151
AcsI	RAATTY	6 cut(s)	244, 559, 952, 1406, 1529, 1685
AcuI	CTGAAG	2 cut(s)	1461, 1548
AfaI	GTAC	3 cut(s)	788, 888, 1275
AfiI	CCNNNNNNNGG	4 cut(s)	78, 240, 666, 1117
AflIII	ACRYGT	2 cut(s)	348, 1628
AgsI	TTSAA	9 cut(s)	122, 287, 610, 703, 1048, 1231, 1323, 1385, 1750
AhdI	GACNNNNNGTC	1 cut(s)	532
AjiI	CACGTC	1 cut(s)	103
AjnI	CCWGG	3 cut(s)	1057, 1347, 1366
AjuI	GAANNNNNNNTTGG	2 cut(s)	731, 763
AleI	CACNNNNGTG	1 cut(s)	693
AloI	GAACNNNNNNTCC	2 cut(s)	414, 446
Alw21I	GWGCWC	4 cut(s)	372, 688, 817, 1040
Alw26I	GTCTC	3 cut(s)	172, 178, 537
AlwI	GGATC	2 cut(s)	1095, 1151
AlwNI	CAGNNNCTG	2 cut(s)	1439, 1524
Ama87I	CYCGRG	1 cut(s)	1112
ApeKI	GCWGC	4 cut(s)	180, 199, 946, 1481
ApoI	RAATTY	6 cut(s)	244, 559, 952, 1406, 1529, 1685
ArsI	GACNNNNNNTTYG	2 cut(s)	628, 660
AspLEI	GCGC	3 cut(s)	164, 775, 1123
AspS9I	GGNCC	2 cut(s)	1359, 1726
AsuHPI	GGTGA	5 cut(s)	100, 587, 649, 812, 983
AsuII	TTCGAA	1 cut(s)	29
AvaI	CYCGRG	1 cut(s)	1112
AvaII	GGWCC	2 cut(s)	1359, 1726
BanII	GRGCYC	1 cut(s)	1040
BauI	CACGAG	1 cut(s)	571
BbsI	GAAGAC	2 cut(s)	110, 584
Bbv12I	GWGCWC	4 cut(s)	372, 688, 817, 1040
BbvI	GCAGC	4 cut(s)	186, 192, 958, 1468
BccI	CCATC	7 cut(s)	50, 283, 644, 803, 928, 965, 1307
BceAI	ACGGC	2 cut(s)	149, 420
BciT130I	CCWGG	3 cut(s)	1059, 1349, 1368
BclI	TGATCA	1 cut(s)	252
BcoDI	GTCTC	3 cut(s)	172, 178, 537
BfaI	CTAG	1 cut(s)	1247
BfoI	RGCGCY	1 cut(s)	1124
BfuAI	ACCTGC	1 cut(s)	929
BisI	GCNGC	4 cut(s)	181, 200, 947, 1482
BlpI	GCTNAGC	2 cut(s)	195, 1181
BlsI	GCNGC	4 cut(s)	182, 201, 948, 1483
Bme1390I	CCNGG	3 cut(s)	1059, 1349, 1368
Bme18I	GGWCC	2 cut(s)	1359, 1726
BmeRI	GACNNNNNGTC	1 cut(s)	532
BmeT110I	CYCGRG	1 cut(s)	1112
BmgBI	CACGTC	1 cut(s)	103
BmgT120I	GGNCC	2 cut(s)	1359, 1726
BmiI	GGNNCC	2 cut(s)	1109, 1727
BmrFI	CCNGG	3 cut(s)	1059, 1349, 1368
BmsI	GCATC	4 cut(s)	53, 838, 1148, 1297
BpiI	GAAGAC	2 cut(s)	110, 584
BplI	GAGNNNNNCTC	2 cut(s)	1348, 1380
BpmI	CTGGAG	1 cut(s)	1602
Bpu1102I	GCTNAGC	2 cut(s)	195, 1181
Bpu14I	TTCGAA	1 cut(s)	29
BpuEI	CTTGAG	2 cut(s)	356, 567
BsaAI	YACGTR	2 cut(s)	858, 1273
BsaJI	CCNNGG	3 cut(s)	436, 994, 1348
BsaWI	WCCGGW	1 cut(s)	707
BsaXI	ACNNNNNCTCC	2 cut(s)	414, 444
Bsc4I	CCNNNNNNNGG	4 cut(s)	78, 240, 666, 1117
Bse1I	ACTGG	2 cut(s)	245, 1160
Bse3DI	GCAATG	1 cut(s)	775
BseBI	CCWGG	3 cut(s)	1059, 1349, 1368
BseDI	CCNNGG	3 cut(s)	436, 994, 1348
BseGI	GGATG	9 cut(s)	42, 68, 216, 814, 853, 1159, 1332, 1423, 1429
BseLI	CCNNNNNNNGG	4 cut(s)	78, 240, 666, 1117
BseMI	GCAATG	1 cut(s)	775
BseMII	CTCAG	6 cut(s)	97, 189, 209, 528, 1195, 1643
BseNI	ACTGG	2 cut(s)	245, 1160
BseRI	GAGGAG	3 cut(s)	140, 270, 313
BseXI	GCAGC	4 cut(s)	186, 192, 958, 1468
BseYI	CCCAGC	1 cut(s)	1103
BsgI	GTGCAG	1 cut(s)	965
Bsh1236I	CGCG	2 cut(s)	897, 1710
BsiHKAI	GWGCWC	4 cut(s)	372, 688, 817, 1040
BsiHKCI	CYCGRG	1 cut(s)	1112
BsiSI	CCGG	1 cut(s)	708
BslFI	GGGAC	3 cut(s)	914, 1280, 1739
BslI	CCNNNNNNNGG	4 cut(s)	78, 240, 666, 1117
BsmAI	GTCTC	3 cut(s)	172, 178, 537
BsmBI	CGTCTC	2 cut(s)	172, 178
BsmFI	GGGAC	3 cut(s)	914, 1280, 1739
BsmI	GAATGC	1 cut(s)	1264
BsoBI	CYCGRG	1 cut(s)	1112
Bsp119I	TTCGAA	1 cut(s)	29
Bsp1286I	GDGCHC	4 cut(s)	372, 688, 817, 1040
Bsp143I	GATC	5 cut(s)	252, 567, 1087, 1156, 1393
Bsp1720I	GCTNAGC	2 cut(s)	195, 1181
Bsp68I	TCGCGA	1 cut(s)	1710
BspACI	CCGC	5 cut(s)	327, 587, 844, 897, 1171
BspCNI	CTCAG	6 cut(s)	96, 188, 208, 529, 1194, 1644
BspFNI	CGCG	2 cut(s)	897, 1710
BspLI	GGNNCC	2 cut(s)	1109, 1727
BspMI	ACCTGC	1 cut(s)	929
BspPI	GGATC	2 cut(s)	1095, 1151
BspQI	GCTCTTC	1 cut(s)	209
BspT104I	TTCGAA	1 cut(s)	29
BsrDI	GCAATG	1 cut(s)	775
BsrI	ACTGG	2 cut(s)	245, 1160
BssECI	CCNNGG	3 cut(s)	436, 994, 1348
BssMI	GATC	5 cut(s)	252, 567, 1087, 1156, 1393
BssSI	CACGAG	1 cut(s)	571
BssT1I	CCWWGG	1 cut(s)	994
Bst2BI	CACGAG	1 cut(s)	571
Bst2UI	CCWGG	3 cut(s)	1059, 1349, 1368
Bst4CI	ACNGT	3 cut(s)	116, 1553, 1580
Bst6I	CTCTTC	2 cut(s)	209, 288
BstAPI	GCANNNNNTGC	1 cut(s)	1145
BstBAI	YACGTR	2 cut(s)	858, 1273
BstBI	TTCGAA	1 cut(s)	29
BstC8I	GCNNGC	3 cut(s)	379, 490, 1590
BstDEI	CTNAG	6 cut(s)	83, 175, 195, 537, 1181, 1652
BstDSI	CCRYGG	1 cut(s)	436
BstENI	CCTNNNNNAGG	1 cut(s)	1115
BstF5I	GGATG	9 cut(s)	42, 68, 216, 814, 853, 1159, 1332, 1423, 1429
BstFNI	CGCG	2 cut(s)	897, 1710
BstH2I	RGCGCY	1 cut(s)	1124
BstHHI	GCGC	3 cut(s)	164, 775, 1123
BstKTI	GATC	5 cut(s)	255, 570, 1090, 1159, 1396
BstMAI	GTCTC	3 cut(s)	172, 178, 537
BstMBI	GATC	5 cut(s)	252, 567, 1087, 1156, 1393
BstMWI	GCNNNNNNNGC	3 cut(s)	1145, 1177, 1307
BstNI	CCWGG	3 cut(s)	1059, 1349, 1368
BstNSI	RCATGY	3 cut(s)	313, 1148, 1632
BstSCI	CCNGG	3 cut(s)	1057, 1347, 1366
BstSNI	TACGTA	1 cut(s)	1273
BstUI	CGCG	2 cut(s)	897, 1710
BstV1I	GCAGC	4 cut(s)	186, 192, 958, 1468
BstV2I	GAAGAC	2 cut(s)	110, 584
BstX2I	RGATCY	1 cut(s)	1087
BstXI	CCANNNNNNTGG	1 cut(s)	1167
BstYI	RGATCY	1 cut(s)	1087
BtgI	CCRYGG	1 cut(s)	436
BtrI	CACGTC	1 cut(s)	103
BtsCI	GGATG	9 cut(s)	42, 68, 216, 814, 853, 1159, 1332, 1423, 1429
BtsI	GCAGTG	1 cut(s)	499
BtsIMutI	CAGTG	2 cut(s)	499, 1585
BtuMI	TCGCGA	1 cut(s)	1710
BveI	ACCTGC	1 cut(s)	929
Cac8I	GCNNGC	3 cut(s)	379, 490, 1590
CaiI	CAGNNNCTG	2 cut(s)	1439, 1524
CfoI	GCGC	3 cut(s)	164, 775, 1123
Cfr13I	GGNCC	2 cut(s)	1359, 1726
Csp6I	GTAC	3 cut(s)	787, 887, 1274
CviQI	GTAC	3 cut(s)	787, 887, 1274
DdeI	CTNAG	6 cut(s)	83, 175, 195, 537, 1181, 1652
DpnI	GATC	5 cut(s)	254, 569, 1089, 1158, 1395
DpnII	GATC	5 cut(s)	252, 567, 1087, 1156, 1393
DriI	GACNNNNNGTC	1 cut(s)	532
Eam1104I	CTCTTC	2 cut(s)	209, 288
Eam1105I	GACNNNNNGTC	1 cut(s)	532
EarI	CTCTTC	2 cut(s)	209, 288
EciI	GGCGGA	1 cut(s)	1186
Ecl136II	GAGCTC	1 cut(s)	1038
Eco105I	TACGTA	1 cut(s)	1273
Eco130I	CCWWGG	1 cut(s)	994
Eco24I	GRGCYC	1 cut(s)	1040
Eco47I	GGWCC	2 cut(s)	1359, 1726
Eco53kI	GAGCTC	1 cut(s)	1038
Eco57I	CTGAAG	2 cut(s)	1461, 1548
Eco88I	CYCGRG	1 cut(s)	1112
EcoICRI	GAGCTC	1 cut(s)	1038
EcoNI	CCTNNNNNAGG	1 cut(s)	1115
EcoRI	GAATTC	3 cut(s)	244, 559, 952
EcoRII	CCWGG	3 cut(s)	1057, 1347, 1366
EcoT14I	CCWWGG	1 cut(s)	994
EcoT22I	ATGCAT	1 cut(s)	723
EcoT38I	GRGCYC	1 cut(s)	1040
ErhI	CCWWGG	1 cut(s)	994
Esp3I	CGTCTC	2 cut(s)	172, 178
FalI	AAGNNNNNCTT	2 cut(s)	576, 608
FaqI	GGGAC	3 cut(s)	914, 1280, 1739
FauI	CCCGC	3 cut(s)	580, 837, 890
FauNDI	CATATG	1 cut(s)	1029
FbaI	TGATCA	1 cut(s)	252
Fnu4HI	GCNGC	4 cut(s)	181, 200, 947, 1482
FokI	GGATG	9 cut(s)	29, 75, 203, 821, 860, 1166, 1339, 1416, 1430
FriOI	GRGCYC	1 cut(s)	1040
Fsp4HI	GCNGC	4 cut(s)	181, 200, 947, 1482
FspBI	CTAG	1 cut(s)	1247
GlaI	GCGC	3 cut(s)	163, 774, 1122
GluI	GCNGC	4 cut(s)	181, 200, 947, 1482
GsaI	CCCAGC	1 cut(s)	1107
GsuI	CTGGAG	1 cut(s)	1602
HaeII	RGCGCY	1 cut(s)	1124
HapII	CCGG	1 cut(s)	708
HhaI	GCGC	3 cut(s)	164, 775, 1123
Hin6I	GCGC	3 cut(s)	162, 773, 1121
HinP1I	GCGC	3 cut(s)	162, 773, 1121
HincII	GTYRAC	1 cut(s)	6
HindII	GTYRAC	1 cut(s)	6
HindIII	AAGCTT	1 cut(s)	24
HinfI	GANTC	7 cut(s)	387, 711, 1174, 1195, 1234, 1250, 1508
HpaII	CCGG	1 cut(s)	708
HphI	GGTGA	5 cut(s)	100, 587, 649, 812, 983
Hpy166II	GTNNAC	4 cut(s)	6, 657, 1402, 1634
Hpy188I	TCNGA	6 cut(s)	86, 257, 334, 538, 738, 1520
Hpy188III	TCNNGA	7 cut(s)	647, 1114, 1247, 1433, 1557, 1619, 1709
Hpy8I	GTNNAC	4 cut(s)	6, 657, 1402, 1634
Hpy99I	CGWCG	1 cut(s)	530
HpyAV	CCTTC	6 cut(s)	28, 141, 733, 1003, 1111, 1616
HpyCH4III	ACNGT	3 cut(s)	116, 1553, 1580
HpyCH4IV	ACGT	6 cut(s)	102, 350, 453, 857, 1206, 1272
HpyF10VI	GCNNNNNNNGC	3 cut(s)	1145, 1177, 1307
HpyF3I	CTNAG	6 cut(s)	83, 175, 195, 537, 1181, 1652
HpySE526I	ACGT	6 cut(s)	102, 350, 453, 857, 1206, 1272
HspAI	GCGC	3 cut(s)	162, 773, 1121
Ksp22I	TGATCA	1 cut(s)	252
Kzo9I	GATC	5 cut(s)	252, 567, 1087, 1156, 1393
LguI	GCTCTTC	1 cut(s)	209
LmnI	GCTCC	3 cut(s)	153, 1043, 1374
Lsp1109I	GCAGC	4 cut(s)	186, 192, 958, 1468
LweI	GCATC	4 cut(s)	53, 838, 1148, 1297
MaeI	CTAG	1 cut(s)	1247
MaeII	ACGT	6 cut(s)	102, 350, 453, 857, 1206, 1272
MaeIII	GTNAC	5 cut(s)	712, 800, 989, 1425, 1574
MalI	GATC	5 cut(s)	254, 569, 1089, 1158, 1395
MboI	GATC	5 cut(s)	252, 567, 1087, 1156, 1393
MboII	GAAGA	7 cut(s)	110, 196, 275, 584, 937, 1243, 1454
MfeI	CAATTG	1 cut(s)	1593
MflI	RGATCY	1 cut(s)	1087
MhlI	GDGCHC	4 cut(s)	372, 688, 817, 1040
MlyI	GAGTC	4 cut(s)	396, 720, 1168, 1259
MmeI	TCCRAC	3 cut(s)	536, 1352, 1498
Mph1103I	ATGCAT	1 cut(s)	723
MseI	TTAA	3 cut(s)	1065, 1341, 1704
MslI	CAYNNNNRTG	1 cut(s)	693
MspA1I	CMGCKG	1 cut(s)	199
MspI	CCGG	1 cut(s)	708
MspR9I	CCNGG	3 cut(s)	1059, 1349, 1368
MunI	CAATTG	1 cut(s)	1593
Mva1269I	GAATGC	1 cut(s)	1264
MvaI	CCWGG	3 cut(s)	1059, 1349, 1368
MvnI	CGCG	2 cut(s)	897, 1710
MwoI	GCNNNNNNNGC	3 cut(s)	1145, 1177, 1307
NdeI	CATATG	1 cut(s)	1029
NdeII	GATC	5 cut(s)	252, 567, 1087, 1156, 1393
NlaIV	GGNNCC	2 cut(s)	1109, 1727
NmeAIII	GCCGAG	1 cut(s)	434
NmuCI	GTSAC	5 cut(s)	712, 800, 989, 1425, 1574
NruI	TCGCGA	1 cut(s)	1710
NsiI	ATGCAT	1 cut(s)	723
NspI	RCATGY	3 cut(s)	313, 1148, 1632
NspV	TTCGAA	1 cut(s)	29
OliI	CACNNNNGTG	1 cut(s)	693
PaeR7I	CTCGAG	1 cut(s)	1112
PaqCI	CACCTGC	1 cut(s)	929
PciI	ACATGT	1 cut(s)	1628
PciSI	GCTCTTC	1 cut(s)	209
PctI	GAATGC	1 cut(s)	1264
PfeI	GAWTC	3 cut(s)	1195, 1234, 1508
PkrI	GCNGC	4 cut(s)	182, 201, 948, 1483
PleI	GAGTC	4 cut(s)	395, 719, 1168, 1258
PpsI	GAGTC	4 cut(s)	395, 719, 1168, 1258
Ppu21I	YACGTR	2 cut(s)	858, 1273
PscI	ACATGT	1 cut(s)	1628
Psp124BI	GAGCTC	1 cut(s)	1040
Psp6I	CCWGG	3 cut(s)	1057, 1347, 1366
PspFI	CCCAGC	1 cut(s)	1103
PspGI	CCWGG	3 cut(s)	1057, 1347, 1366
PspN4I	GGNNCC	2 cut(s)	1109, 1727
PspPI	GGNCC	2 cut(s)	1359, 1726
PstNI	CAGNNNCTG	2 cut(s)	1439, 1524
PsuI	RGATCY	1 cut(s)	1087
PvuII	CAGCTG	1 cut(s)	199
RruI	TCGCGA	1 cut(s)	1710
RsaI	GTAC	3 cut(s)	788, 888, 1275
RsaNI	GTAC	3 cut(s)	787, 887, 1274
RseI	CAYNNNNRTG	1 cut(s)	693
SacI	GAGCTC	1 cut(s)	1040
SapI	GCTCTTC	1 cut(s)	209
SaqAI	TTAA	3 cut(s)	1065, 1341, 1704
SatI	GCNGC	4 cut(s)	181, 200, 947, 1482
Sau3AI	GATC	5 cut(s)	252, 567, 1087, 1156, 1393
Sau96I	GGNCC	2 cut(s)	1359, 1726
SchI	GAGTC	4 cut(s)	396, 720, 1168, 1259
ScrFI	CCNGG	3 cut(s)	1059, 1349, 1368
SduI	GDGCHC	4 cut(s)	372, 688, 817, 1040
SfaNI	GCATC	4 cut(s)	53, 838, 1148, 1297
Sfr274I	CTCGAG	1 cut(s)	1112
SfuI	TTCGAA	1 cut(s)	29
SinI	GGWCC	2 cut(s)	1359, 1726
SlaI	CTCGAG	1 cut(s)	1112
SmiMI	CAYNNNNRTG	1 cut(s)	693
SmlI	CTYRAG	3 cut(s)	371, 582, 1112
SmoI	CTYRAG	3 cut(s)	371, 582, 1112
SnaBI	TACGTA	1 cut(s)	1273
SsiI	CCGC	5 cut(s)	327, 587, 844, 897, 1171
SspMI	CTAG	1 cut(s)	1247
SstI	GAGCTC	1 cut(s)	1040
StyD4I	CCNGG	3 cut(s)	1057, 1347, 1366
StyI	CCWWGG	1 cut(s)	994
TaaI	ACNGT	3 cut(s)	116, 1553, 1580
TaiI	ACGT	6 cut(s)	105, 353, 456, 860, 1209, 1275
TaqI	TCGA	6 cut(s)	29, 525, 1099, 1113, 1256, 1453
TaqII	GACCGA	1 cut(s)	1743
TatI	WGTACW	1 cut(s)	886
TfiI	GAWTC	3 cut(s)	1195, 1234, 1508
Tru1I	TTAA	3 cut(s)	1065, 1341, 1704
Tru9I	TTAA	3 cut(s)	1065, 1341, 1704
TscAI	CASTG	2 cut(s)	499, 1585
TseFI	GTSAC	5 cut(s)	712, 800, 989, 1425, 1574
TseI	GCWGC	4 cut(s)	180, 199, 946, 1481
Tsp45I	GTSAC	5 cut(s)	712, 800, 989, 1425, 1574
TspDTI	ATGAA	8 cut(s)	407, 491, 738, 1115, 1187, 1560, 1665, 1730
TspGWI	ACGGA	4 cut(s)	160, 529, 1232, 1651
TspRI	CASTG	2 cut(s)	499, 1585
VpaK11BI	GGWCC	2 cut(s)	1359, 1726
XagI	CCTNNNNNAGG	1 cut(s)	1115
XapI	RAATTY	6 cut(s)	244, 559, 952, 1406, 1529, 1685
XbaI	TCTAGA	1 cut(s)	1246
XceI	RCATGY	3 cut(s)	313, 1148, 1632
XhoI	CTCGAG	1 cut(s)	1112
XspI	CTAG	1 cut(s)	1247
Zsp2I	ATGCAT	1 cut(s)	723

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.