Rroxscaffold_6G00420760

Pentatricopeptide repeat-containing protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000006
OG0014411
Physical Location & Seq
Reverse (-)
41976608 .. 41979157
2550 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_6G00420760.1

Sequence Viewer

Length: 1788 bp
ATGTTGACAAACCATACCTTCCAAGCTTCGAAAACCAAAGCCATCCTAACCCTCTTGCAAGGATGCAACACCCTCAAAAGGCTCAGAAAAATCCACGCCCACGTCATCACCAACGGTCTTCAACACCACCCTTCCATTTCCAACAAGCTCCTCAACTTCTGCGCTGTCTCCGTCTCAGGCAGCTTACCCTACGCTCAGCTTCTGTTCCATTATCACATCCCAAACCCACAAACCCAAGACTGGAATTCCATGATCAGAGGCTTCTCCCAAAGCCCATCTCCTCTTCAAGCCATTTCTTACTACAACCACATGCTTTCGTCCTCCTCCTCCGCTTCCGATTGCCCACCCGACACTTTTAGCTTCTCGTTTGTGCTCAAGGCTTGCGAGAGAGTCAGGGCAGAGAGCAAGTGCCGAGAGGTTCATGGAGTTGTGGTTCGCCATGGTTATGATATGGACGTTGTTGTTTGCACCAATTTGATGAAGTCTTATGCTGGCAATGGGTCAGTTGATAATGCACGGAGAGTGTTCGACGAAATGTCTGAGAGAGACTTGGTGTGTTGGAATTCCATGATCTCGTGCTACTCTCAAGCGGGTCTTCACCATGAGGCGTTGAATATATATAGTCTTATGAGAAATCAAAATGTGGATCTTGATGGGTTCACTCTTGTTGGGTTGCTTTCGTCTTCTGCTCACCTGGGTGCGTTGAACACCGGAGTCACGATGCATGAACTTGCTCGTGAGAAGGGGTTTTTGGGAAGTGTTTATGTTGGCAATGCGCTTATAGATATGTATGCAAAATGTGGTGACTTGGATGGTGCTCTTTGTGTCTTTGAGAGAATGCGAAAGCGGGATGCTTTTACATGGAACTCCATGATTGTTGGGTATGGAGTACATGGTCGTGGGGACGAAGCAATCTATTTTTTTGAGAAGATGATGATGGCAGGTGTTCAGCCGAATTCCATCACATTTCTCGGGTTGTTGTGTGGGTGTAGTCACCAAGGATTGGTAGAGAAGGGTGTTGATTACTTCCATATGATGAGCTCCAAGTTCAATATCAAACCTGGAATTAAGCACTATGGGTGTCTGGTGGATCTGTTTGGTCGAGCTGGGAAGCTCGAGAAGGCGCTTCATGTTATAAGGACATCACATGCACAGGATGATCCAGTTCTATGGCGGACTCTGCTCAGCTCTTGCAAGATTCATAAAAACGTGGAAATAGGAGAAGTTGCCTTGAAGAATCTAATCCGTTTAGGGTCATCAAATGCAGGGGACTACGTACTTCTTGCTACTATTTATTTTAGGGCGAAAGATGCAGATGGTGTTTCAAGGATGAGAAAATTGATTAAAAACCAGGGAGTGAGGACCACTCCTGGTTGGAGCTGGATTGAAGTTGGCGATCAGGTTCACAAATTTGTAGTGGATGACAAGTCACATCCAGATACTGAAGAAATCTATCGAAAATTGGAGGTAGTAGTCCAACGAGCTGCTCTACATGGTTATCTGCAAGAGGAATCTCTTATCAGAGCCTGTGAATTTACCTCCATAGACACCAACTGTTCGGGAACTTCTGGTTCATGTCACAGTGAGAAGCTGGCAATTGCTTTTGGCTTGGCAAGAACTCCACAAGGAACATGTTTACGGATAGTAAAAAACCTGAGAGTTTGTAGAGATTGCCATTCATTTACAAAATTTGTTTCCCAAGCATTTAATCGAGAAATAATTGTTAGGGACCGAGTTCGGTTTCATCACTTCAAGGGTGGATTGTGTAGTTGTAAAGACTATTGGTGA

Protein Analysis

595

Amino Acids

66.59

Weight (kDa)

8.83

Isoelectric Point (pI)

35.79

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PPR_2 PF13041 183 - 227 2.9e-07 PPR repeat family
PPR PF01535 185 - 214 3.3e-07 PPR repeat
PPR_2 PF13041 284 - 331 7.9e-11 PPR repeat family
PPR PF01535 286 - 316 4.3e-07 PPR repeat
E_motif PF20431 401 - 463 2.5e-14 E motif
Eplus_motif PF20430 468 - 494 9.7e-07 E+ motif
DYW_deaminase PF14432 526 - 595 1.5e-33 DYW family of nucleic acid deaminases
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0014411)

Species Orthologous Gene IDs
arabidopsis_thaliana AT3G56550 AT3G56550
fragaria_vesca FvH4_6g08690
malus_domestica MD04G1184300.v1.1 MD12G1197500.v1.1
prunus_persica Prupe.6G302800_v2.0.a1
pyrus_communis pycom04g16330 pycom12g18380
rosa_chinensis RchiOBHm_Chr3g0458831
rosa_laevigata RLG00000025132
rosa_multiflora Rmu_co8385739.1_g000001
rosa_roxburghii Rroxscaffold_6G00420760
rosa_rugosa Rorug03G0032200
rosa_samantha Rh3BG092700 Rh3CG093000 Rh3DG093700
rosa_wichuraiana Rw3G007690

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 1136
AarI CACCTGC 1 cut(s) 932
Acc36I ACCTGC 1 cut(s) 932
AccB7I CCANNNNNTGG 1 cut(s) 1003
AciI CCGC 4 cut(s) 330, 590, 847, 1174
AclWI GGATC 3 cut(s) 654, 1098, 1154
AcsI RAATTY 6 cut(s) 244, 562, 955, 1409, 1532, 1688
AcuI CTGAAG 1 cut(s) 1464
AfaI GTAC 2 cut(s) 891, 1278
AfiI CCNNNNNNNGG 3 cut(s) 78, 240, 1003
AflIII ACRYGT 1 cut(s) 1631
AgsI TTSAA 9 cut(s) 122, 287, 613, 706, 1051, 1234, 1326, 1388, 1753
AhdI GACNNNNNGTC 1 cut(s) 535
AjiI CACGTC 1 cut(s) 103
AjnI CCWGG 4 cut(s) 693, 1060, 1350, 1369
AjuI GAANNNNNNNTTGG 2 cut(s) 734, 766
AleI CACNNNNGTG 1 cut(s) 696
AloI GAACNNNNNNTCC 2 cut(s) 417, 449
Alw21I GWGCWC 3 cut(s) 375, 820, 1043
Alw26I GTCTC 3 cut(s) 172, 178, 540
AlwI GGATC 3 cut(s) 654, 1098, 1154
AlwNI CAGNNNCTG 3 cut(s) 202, 1442, 1527
Ama87I CYCGRG 2 cut(s) 971, 1115
ApeKI GCWGC 2 cut(s) 180, 1484
ApoI RAATTY 6 cut(s) 244, 562, 955, 1409, 1532, 1688
AspLEI GCGC 3 cut(s) 164, 778, 1126
AspS9I GGNCC 2 cut(s) 1362, 1729
AsuHPI GGTGA 5 cut(s) 100, 590, 683, 815, 986
AsuII TTCGAA 1 cut(s) 29
AvaI CYCGRG 2 cut(s) 971, 1115
AvaII GGWCC 2 cut(s) 1362, 1729
BanII GRGCYC 1 cut(s) 1043
BauI CACGAG 2 cut(s) 574, 735
BbsI GAAGAC 3 cut(s) 110, 587, 675
Bbv12I GWGCWC 3 cut(s) 375, 820, 1043
BbvI GCAGC 2 cut(s) 192, 1471
BccI CCATC 7 cut(s) 50, 283, 647, 806, 931, 968, 1310
BciT130I CCWGG 4 cut(s) 695, 1062, 1352, 1371
BclI TGATCA 1 cut(s) 252
BcoDI GTCTC 3 cut(s) 172, 178, 540
BfoI RGCGCY 1 cut(s) 1127
BfuAI ACCTGC 1 cut(s) 932
BisI GCNGC 2 cut(s) 181, 1485
BlpI GCTNAGC 2 cut(s) 195, 1184
BlsI GCNGC 2 cut(s) 182, 1486
Bme1390I CCNGG 4 cut(s) 695, 1062, 1352, 1371
Bme18I GGWCC 2 cut(s) 1362, 1729
BmeRI GACNNNNNGTC 1 cut(s) 535
BmeT110I CYCGRG 2 cut(s) 971, 1115
BmgBI CACGTC 1 cut(s) 103
BmgT120I GGNCC 2 cut(s) 1362, 1729
BmiI GGNNCC 1 cut(s) 1730
BmrFI CCNGG 4 cut(s) 695, 1062, 1352, 1371
BmsI GCATC 4 cut(s) 53, 711, 841, 1300
BpiI GAAGAC 3 cut(s) 110, 587, 675
BplI GAGNNNNNCTC 2 cut(s) 1351, 1383
Bpu1102I GCTNAGC 2 cut(s) 195, 1184
Bpu14I TTCGAA 1 cut(s) 29
BpuEI CTTGAG 2 cut(s) 359, 570
BsaAI YACGTR 1 cut(s) 1276
BsaJI CCNNGG 4 cut(s) 439, 694, 997, 1351
BsaWI WCCGGW 1 cut(s) 710
BsaXI ACNNNNNCTCC 2 cut(s) 417, 447
Bsc4I CCNNNNNNNGG 3 cut(s) 78, 240, 1003
Bse1I ACTGG 2 cut(s) 245, 1163
Bse3DI GCAATG 2 cut(s) 502, 778
BseBI CCWGG 4 cut(s) 695, 1062, 1352, 1371
BseDI CCNNGG 4 cut(s) 439, 694, 997, 1351
BseGI GGATG 9 cut(s) 42, 68, 216, 817, 856, 1162, 1335, 1426, 1432
BseLI CCNNNNNNNGG 3 cut(s) 78, 240, 1003
BseMI GCAATG 2 cut(s) 502, 778
BseMII CTCAG 6 cut(s) 97, 189, 209, 531, 1198, 1646
BseNI ACTGG 2 cut(s) 245, 1163
BseRI GAGGAG 4 cut(s) 140, 270, 313, 316
BseXI GCAGC 2 cut(s) 192, 1471
BseYI CCCAGC 1 cut(s) 1106
BsiHKAI GWGCWC 3 cut(s) 375, 820, 1043
BsiHKCI CYCGRG 2 cut(s) 971, 1115
BsiSI CCGG 1 cut(s) 711
BslFI GGGAC 3 cut(s) 917, 1283, 1742
BslI CCNNNNNNNGG 3 cut(s) 78, 240, 1003
BsmAI GTCTC 3 cut(s) 172, 178, 540
BsmBI CGTCTC 1 cut(s) 178
BsmFI GGGAC 3 cut(s) 917, 1283, 1742
BsmI GAATGC 1 cut(s) 843
BsoBI CYCGRG 2 cut(s) 971, 1115
Bsp119I TTCGAA 1 cut(s) 29
Bsp1286I GDGCHC 3 cut(s) 375, 820, 1043
Bsp143I GATC 6 cut(s) 252, 570, 646, 1090, 1159, 1396
Bsp1720I GCTNAGC 2 cut(s) 195, 1184
Bsp19I CCATGG 1 cut(s) 439
BspACI CCGC 4 cut(s) 330, 590, 847, 1174
BspCNI CTCAG 6 cut(s) 96, 188, 208, 532, 1197, 1647
BspLI GGNNCC 1 cut(s) 1730
BspMI ACCTGC 1 cut(s) 932
BspPI GGATC 3 cut(s) 654, 1098, 1154
BspT104I TTCGAA 1 cut(s) 29
BsrDI GCAATG 2 cut(s) 502, 778
BsrI ACTGG 2 cut(s) 245, 1163
BssECI CCNNGG 4 cut(s) 439, 694, 997, 1351
BssMI GATC 6 cut(s) 252, 570, 646, 1090, 1159, 1396
BssSI CACGAG 2 cut(s) 574, 735
BssT1I CCWWGG 2 cut(s) 439, 997
Bst2BI CACGAG 2 cut(s) 574, 735
Bst2UI CCWGG 4 cut(s) 695, 1062, 1352, 1371
Bst4CI ACNGT 3 cut(s) 116, 1556, 1583
Bst6I CTCTTC 1 cut(s) 288
BstBAI YACGTR 1 cut(s) 1276
BstBI TTCGAA 1 cut(s) 29
BstC8I GCNNGC 3 cut(s) 382, 493, 1593
BstDEI CTNAG 6 cut(s) 83, 175, 195, 540, 1184, 1655
BstDSI CCRYGG 1 cut(s) 439
BstF5I GGATG 9 cut(s) 42, 68, 216, 817, 856, 1162, 1335, 1426, 1432
BstH2I RGCGCY 1 cut(s) 1127
BstHHI GCGC 3 cut(s) 164, 778, 1126
BstKTI GATC 6 cut(s) 255, 573, 649, 1093, 1162, 1399
BstMAI GTCTC 3 cut(s) 172, 178, 540
BstMBI GATC 6 cut(s) 252, 570, 646, 1090, 1159, 1396
BstMWI GCNNNNNNNGC 2 cut(s) 1180, 1310
BstNI CCWGG 4 cut(s) 695, 1062, 1352, 1371
BstNSI RCATGY 3 cut(s) 313, 1151, 1635
BstSCI CCNGG 4 cut(s) 693, 1060, 1350, 1369
BstSNI TACGTA 1 cut(s) 1276
BstV1I GCAGC 2 cut(s) 192, 1471
BstV2I GAAGAC 3 cut(s) 110, 587, 675
BstX2I RGATCY 2 cut(s) 646, 1090
BstXI CCANNNNNNTGG 1 cut(s) 1170
BstYI RGATCY 2 cut(s) 646, 1090
BtgI CCRYGG 1 cut(s) 439
BtrI CACGTC 1 cut(s) 103
BtsCI GGATG 9 cut(s) 42, 68, 216, 817, 856, 1162, 1335, 1426, 1432
BtsIMutI CAGTG 1 cut(s) 1588
BveI ACCTGC 1 cut(s) 932
Cac8I GCNNGC 3 cut(s) 382, 493, 1593
CaiI CAGNNNCTG 3 cut(s) 202, 1442, 1527
CfoI GCGC 3 cut(s) 164, 778, 1126
Cfr13I GGNCC 2 cut(s) 1362, 1729
Csp6I GTAC 2 cut(s) 890, 1277
CviQI GTAC 2 cut(s) 890, 1277
DdeI CTNAG 6 cut(s) 83, 175, 195, 540, 1184, 1655
DpnI GATC 6 cut(s) 254, 572, 648, 1092, 1161, 1398
DpnII GATC 6 cut(s) 252, 570, 646, 1090, 1159, 1396
DriI GACNNNNNGTC 1 cut(s) 535
Eam1104I CTCTTC 1 cut(s) 288
Eam1105I GACNNNNNGTC 1 cut(s) 535
EarI CTCTTC 1 cut(s) 288
EciI GGCGGA 1 cut(s) 1189
Ecl136II GAGCTC 1 cut(s) 1041
Eco105I TACGTA 1 cut(s) 1276
Eco130I CCWWGG 2 cut(s) 439, 997
Eco24I GRGCYC 1 cut(s) 1043
Eco47I GGWCC 2 cut(s) 1362, 1729
Eco53kI GAGCTC 1 cut(s) 1041
Eco57I CTGAAG 1 cut(s) 1464
Eco88I CYCGRG 2 cut(s) 971, 1115
EcoICRI GAGCTC 1 cut(s) 1041
EcoRI GAATTC 3 cut(s) 244, 562, 955
EcoRII CCWGG 4 cut(s) 693, 1060, 1350, 1369
EcoT14I CCWWGG 2 cut(s) 439, 997
EcoT22I ATGCAT 1 cut(s) 726
EcoT38I GRGCYC 1 cut(s) 1043
ErhI CCWWGG 2 cut(s) 439, 997
Esp3I CGTCTC 1 cut(s) 178
FalI AAGNNNNNCTT 2 cut(s) 579, 611
FaqI GGGAC 3 cut(s) 917, 1283, 1742
FauI CCCGC 2 cut(s) 583, 840
FauNDI CATATG 1 cut(s) 1032
FbaI TGATCA 1 cut(s) 252
Fnu4HI GCNGC 2 cut(s) 181, 1485
FokI GGATG 9 cut(s) 29, 75, 203, 824, 863, 1169, 1342, 1419, 1433
FriOI GRGCYC 1 cut(s) 1043
Fsp4HI GCNGC 2 cut(s) 181, 1485
GlaI GCGC 3 cut(s) 163, 777, 1125
GluI GCNGC 2 cut(s) 181, 1485
GsaI CCCAGC 1 cut(s) 1110
HaeII RGCGCY 1 cut(s) 1127
HapII CCGG 1 cut(s) 711
HhaI GCGC 3 cut(s) 164, 778, 1126
Hin6I GCGC 3 cut(s) 162, 776, 1124
HinP1I GCGC 3 cut(s) 162, 776, 1124
HincII GTYRAC 1 cut(s) 6
HindII GTYRAC 1 cut(s) 6
HindIII AAGCTT 1 cut(s) 24
HinfI GANTC 6 cut(s) 390, 714, 1177, 1198, 1237, 1511
HpaII CCGG 1 cut(s) 711
HphI GGTGA 5 cut(s) 100, 590, 683, 815, 986
Hpy166II GTNNAC 4 cut(s) 6, 660, 1405, 1637
Hpy188I TCNGA 5 cut(s) 86, 257, 337, 541, 1523
Hpy188III TCNNGA 7 cut(s) 650, 718, 737, 1117, 1436, 1560, 1712
Hpy8I GTNNAC 4 cut(s) 6, 660, 1405, 1637
Hpy99I CGWCG 1 cut(s) 533
HpyAV CCTTC 5 cut(s) 28, 141, 736, 1006, 1114
HpyCH4III ACNGT 3 cut(s) 116, 1556, 1583
HpyCH4IV ACGT 4 cut(s) 102, 456, 1209, 1275
HpyF10VI GCNNNNNNNGC 2 cut(s) 1180, 1310
HpyF3I CTNAG 6 cut(s) 83, 175, 195, 540, 1184, 1655
HpySE526I ACGT 4 cut(s) 102, 456, 1209, 1275
HspAI GCGC 3 cut(s) 162, 776, 1124
Ksp22I TGATCA 1 cut(s) 252
Kzo9I GATC 6 cut(s) 252, 570, 646, 1090, 1159, 1396
LmnI GCTCC 3 cut(s) 153, 1046, 1377
Lsp1109I GCAGC 2 cut(s) 192, 1471
LweI GCATC 4 cut(s) 53, 711, 841, 1300
MaeII ACGT 4 cut(s) 102, 456, 1209, 1275
MaeIII GTNAC 5 cut(s) 715, 803, 992, 1428, 1577
MalI GATC 6 cut(s) 254, 572, 648, 1092, 1161, 1398
MboI GATC 6 cut(s) 252, 570, 646, 1090, 1159, 1396
MboII GAAGA 7 cut(s) 110, 275, 587, 675, 940, 1246, 1457
MfeI CAATTG 1 cut(s) 1596
MflI RGATCY 2 cut(s) 646, 1090
MhlI GDGCHC 3 cut(s) 375, 820, 1043
MlyI GAGTC 3 cut(s) 399, 723, 1171
MmeI TCCRAC 4 cut(s) 165, 539, 1355, 1501
Mph1103I ATGCAT 1 cut(s) 726
MseI TTAA 3 cut(s) 1068, 1344, 1707
MslI CAYNNNNRTG 3 cut(s) 444, 696, 897
MspI CCGG 1 cut(s) 711
MspR9I CCNGG 4 cut(s) 695, 1062, 1352, 1371
MunI CAATTG 1 cut(s) 1596
Mva1269I GAATGC 1 cut(s) 843
MvaI CCWGG 4 cut(s) 695, 1062, 1352, 1371
MwoI GCNNNNNNNGC 2 cut(s) 1180, 1310
NcoI CCATGG 1 cut(s) 439
NdeI CATATG 1 cut(s) 1032
NdeII GATC 6 cut(s) 252, 570, 646, 1090, 1159, 1396
NlaIV GGNNCC 1 cut(s) 1730
NmeAIII GCCGAG 1 cut(s) 437
NmuCI GTSAC 5 cut(s) 715, 803, 992, 1428, 1577
NsiI ATGCAT 1 cut(s) 726
NspI RCATGY 3 cut(s) 313, 1151, 1635
NspV TTCGAA 1 cut(s) 29
OliI CACNNNNGTG 1 cut(s) 696
PaeR7I CTCGAG 1 cut(s) 1115
PaqCI CACCTGC 1 cut(s) 932
PciI ACATGT 1 cut(s) 1631
PctI GAATGC 1 cut(s) 843
PfeI GAWTC 3 cut(s) 1198, 1237, 1511
PflMI CCANNNNNTGG 1 cut(s) 1003
PkrI GCNGC 2 cut(s) 182, 1486
PleI GAGTC 3 cut(s) 398, 722, 1171
PpsI GAGTC 3 cut(s) 398, 722, 1171
Ppu21I YACGTR 1 cut(s) 1276
PscI ACATGT 1 cut(s) 1631
PsiI TTATAA 1 cut(s) 1136
Psp124BI GAGCTC 1 cut(s) 1043
Psp6I CCWGG 4 cut(s) 693, 1060, 1350, 1369
PspFI CCCAGC 1 cut(s) 1106
PspGI CCWGG 4 cut(s) 693, 1060, 1350, 1369
PspN4I GGNNCC 1 cut(s) 1730
PspPI GGNCC 2 cut(s) 1362, 1729
PstNI CAGNNNCTG 3 cut(s) 202, 1442, 1527
PsuI RGATCY 2 cut(s) 646, 1090
RsaI GTAC 2 cut(s) 891, 1278
RsaNI GTAC 2 cut(s) 890, 1277
RseI CAYNNNNRTG 3 cut(s) 444, 696, 897
SacI GAGCTC 1 cut(s) 1043
SaqAI TTAA 3 cut(s) 1068, 1344, 1707
SatI GCNGC 2 cut(s) 181, 1485
Sau3AI GATC 6 cut(s) 252, 570, 646, 1090, 1159, 1396
Sau96I GGNCC 2 cut(s) 1362, 1729
SchI GAGTC 3 cut(s) 399, 723, 1171
ScrFI CCNGG 4 cut(s) 695, 1062, 1352, 1371
SduI GDGCHC 3 cut(s) 375, 820, 1043
SfaNI GCATC 4 cut(s) 53, 711, 841, 1300
Sfr274I CTCGAG 1 cut(s) 1115
SfuI TTCGAA 1 cut(s) 29
SinI GGWCC 2 cut(s) 1362, 1729
SlaI CTCGAG 1 cut(s) 1115
SmiMI CAYNNNNRTG 3 cut(s) 444, 696, 897
SmlI CTYRAG 3 cut(s) 374, 585, 1115
SmoI CTYRAG 3 cut(s) 374, 585, 1115
SnaBI TACGTA 1 cut(s) 1276
SsiI CCGC 4 cut(s) 330, 590, 847, 1174
SstI GAGCTC 1 cut(s) 1043
StyD4I CCNGG 4 cut(s) 693, 1060, 1350, 1369
StyI CCWWGG 2 cut(s) 439, 997
TaaI ACNGT 3 cut(s) 116, 1556, 1583
TaiI ACGT 4 cut(s) 105, 459, 1212, 1278
TaqI TCGA 6 cut(s) 29, 528, 1102, 1116, 1456, 1711
TaqII GACCGA 1 cut(s) 1746
TatI WGTACW 1 cut(s) 889
TfiI GAWTC 3 cut(s) 1198, 1237, 1511
Tru1I TTAA 3 cut(s) 1068, 1344, 1707
Tru9I TTAA 3 cut(s) 1068, 1344, 1707
TscAI CASTG 1 cut(s) 1588
TseFI GTSAC 5 cut(s) 715, 803, 992, 1428, 1577
TseI GCWGC 2 cut(s) 180, 1484
Tsp45I GTSAC 5 cut(s) 715, 803, 992, 1428, 1577
TspDTI ATGAA 8 cut(s) 410, 494, 741, 1118, 1190, 1563, 1668, 1733
TspGWI ACGGA 4 cut(s) 160, 532, 1235, 1654
TspRI CASTG 1 cut(s) 1588
Van91I CCANNNNNTGG 1 cut(s) 1003
VpaK11BI GGWCC 2 cut(s) 1362, 1729
XapI RAATTY 6 cut(s) 244, 562, 955, 1409, 1532, 1688
XceI RCATGY 3 cut(s) 313, 1151, 1635
XhoI CTCGAG 1 cut(s) 1115
Zsp2I ATGCAT 1 cut(s) 726
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.