MD04G1203300.v1.1

Nuclear transcription factor Y subunit

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr04
OG0015296
Physical Location & Seq
Forward (+)
28953974 .. 28954426
453 bp
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UTR
Exon/CDS
Intron
MD04G1203300.v1.1.491

Sequence Viewer

Length: 453 bp
ATGGATCAATCAATCAACATGGTTGATCAGGAACAAGATGGGATGCTCCCAATTGCAAACGTGGGTCGAATCATGAAGCAAATTCTTCCCCCAAGAGCCAAAATTTCCAAGGAGGCGAAACAAACAATGCAAGAATGTGCAACTGAGTTCCTAAGTTTTGTGACTGGCGAGGCTTCTGACAAGTGTCACAAGGAGAATCGCAAGACCGTCAATGGAGACGACATCTGTTGGGCTCTGAGTGCTCTAGGGTTTGATAACTATGCCCAGGCTGCTATGAGGTACTTGCATAAATATAGGGAAACTGAAAGAGACAAAGCTGCTGCTAACAACAACCAAAACACGGCAAATATTACAAGTCATGGAGGCCTGCAAGTAAGGGAGCAAACTCAGACTTCAAATCTGGAGTTTCGGGTGCTTGAGAAAGGTGACAACTCCCTTATTACCAAGCCATGA

Protein Analysis

151

Amino Acids

16.86

Weight (kDa)

6.3

Isoelectric Point (pI)

34.43

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
CBFD_NFYB_HMF PF00808 16 - 79 1.6e-25 Histone-like transcription factor (CBF/NF-Y) and archaeal histone
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0015296)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G09030
fragaria_vesca FvH4_6g06220
malus_domestica MD04G1203300.v1.1 MD12G1217100.v1.1
prunus_persica Prupe.6G323400_v2.0.a1
pyrus_communis pycom04g17970
rosa_chinensis RchiOBHm_Chr3g0455241
rosa_laevigata RLG00000025374
rosa_multiflora Rmu_sc0002169.1_g000034
rosa_roxburghii Rroxscaffold_6G00424430
rosa_rugosa Rorug03G0005500
rosa_samantha Rh3AG065500 Rh3BG067600 Rh3CG066100 Rh3DG067700
rosa_wichuraiana Rw3G005160

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AclWI GGATC 1 cut(s) 12
AcsI RAATTY 2 cut(s) 81, 102
AfaI GTAC 1 cut(s) 281
AfiI CCNNNNNNNGG 1 cut(s) 340
AgsI TTSAA 1 cut(s) 396
AjnI CCWGG 1 cut(s) 264
AluBI AGCT 1 cut(s) 317
AluI AGCT 1 cut(s) 317
Alw21I GWGCWC 1 cut(s) 244
Alw26I GTCTC 2 cut(s) 210, 303
AlwI GGATC 1 cut(s) 12
AoxI GGCC 1 cut(s) 364
ApeKI GCWGC 3 cut(s) 269, 317, 320
ApoI RAATTY 2 cut(s) 81, 102
AsuHPI GGTGA 1 cut(s) 437
BanII GRGCYC 1 cut(s) 235
Bbv12I GWGCWC 1 cut(s) 244
BbvI GCAGC 3 cut(s) 256, 304, 307
BccI CCATC 1 cut(s) 32
BceAI ACGGC 1 cut(s) 357
BciT130I CCWGG 1 cut(s) 266
BclI TGATCA 1 cut(s) 25
BcoDI GTCTC 2 cut(s) 210, 303
BfaI CTAG 1 cut(s) 245
BisI GCNGC 3 cut(s) 270, 318, 321
BlsI GCNGC 3 cut(s) 271, 319, 322
Bme1390I CCNGG 1 cut(s) 266
BmrFI CCNGG 1 cut(s) 266
BmsI GCATC 1 cut(s) 33
BoxI GACNNNNGTC 1 cut(s) 183
BpmI CTGGAG 1 cut(s) 422
BpuEI CTTGAG 1 cut(s) 437
BsaJI CCNNGG 2 cut(s) 108, 264
Bsc4I CCNNNNNNNGG 1 cut(s) 340
Bse1I ACTGG 1 cut(s) 169
BseBI CCWGG 1 cut(s) 266
BseDI CCNNGG 2 cut(s) 108, 264
BseGI GGATG 1 cut(s) 48
BseLI CCNNNNNNNGG 1 cut(s) 340
BseMII CTCAG 3 cut(s) 135, 227, 401
BseNI ACTGG 1 cut(s) 169
BseXI GCAGC 3 cut(s) 256, 304, 307
BshFI GGCC 1 cut(s) 366
BsiHKAI GWGCWC 1 cut(s) 244
BslI CCNNNNNNNGG 1 cut(s) 340
BsmAI GTCTC 2 cut(s) 210, 303
BsmBI CGTCTC 1 cut(s) 210
BsnI GGCC 1 cut(s) 366
Bsp1286I GDGCHC 2 cut(s) 235, 244
Bsp143I GATC 2 cut(s) 4, 25
BspANI GGCC 1 cut(s) 366
BspCNI CTCAG 3 cut(s) 136, 228, 400
BspHI TCATGA 1 cut(s) 72
BspPI GGATC 1 cut(s) 12
BsrI ACTGG 1 cut(s) 169
BssECI CCNNGG 2 cut(s) 108, 264
BssMI GATC 2 cut(s) 4, 25
BssT1I CCWWGG 1 cut(s) 108
Bst2UI CCWGG 1 cut(s) 266
Bst4CI ACNGT 1 cut(s) 208
BstC8I GCNNGC 1 cut(s) 368
BstDEI CTNAG 4 cut(s) 144, 152, 236, 387
BstF5I GGATG 1 cut(s) 48
BstKTI GATC 2 cut(s) 7, 28
BstMAI GTCTC 2 cut(s) 210, 303
BstMBI GATC 2 cut(s) 4, 25
BstMWI GCNNNNNNNGC 2 cut(s) 239, 269
BstNI CCWGG 1 cut(s) 266
BstPAI GACNNNNGTC 1 cut(s) 183
BstSCI CCNGG 1 cut(s) 264
BstV1I GCAGC 3 cut(s) 256, 304, 307
BsuRI GGCC 1 cut(s) 366
BtsCI GGATG 1 cut(s) 48
Cac8I GCNNGC 1 cut(s) 368
CciI TCATGA 1 cut(s) 72
Csp6I GTAC 1 cut(s) 280
CviAII CATG 4 cut(s) 19, 73, 359, 450
CviJI RGCY 7 cut(s) 98, 173, 233, 269, 317, 366, 448
CviKI_1 RGCY 7 cut(s) 98, 173, 233, 269, 317, 366, 448
CviQI GTAC 1 cut(s) 280
DdeI CTNAG 4 cut(s) 144, 152, 236, 387
DpnI GATC 2 cut(s) 6, 27
DpnII GATC 2 cut(s) 4, 25
Eco130I CCWWGG 1 cut(s) 108
Eco147I AGGCCT 1 cut(s) 366
Eco24I GRGCYC 1 cut(s) 235
EcoRII CCWGG 1 cut(s) 264
EcoT14I CCWWGG 1 cut(s) 108
EcoT38I GRGCYC 1 cut(s) 235
ErhI CCWWGG 1 cut(s) 108
Esp3I CGTCTC 1 cut(s) 210
FaeI CATG 4 cut(s) 22, 76, 362, 453
FaiI YATR 8 cut(s) 20, 74, 261, 275, 288, 294, 360, 451
FatI CATG 4 cut(s) 18, 72, 358, 449
FbaI TGATCA 1 cut(s) 25
Fnu4HI GCNGC 3 cut(s) 270, 318, 321
FokI GGATG 1 cut(s) 55
FriOI GRGCYC 1 cut(s) 235
Fsp4HI GCNGC 3 cut(s) 270, 318, 321
FspBI CTAG 1 cut(s) 245
GluI GCNGC 3 cut(s) 270, 318, 321
GsuI CTGGAG 1 cut(s) 422
HaeIII GGCC 1 cut(s) 366
Hin1II CATG 4 cut(s) 22, 76, 362, 453
HinfI GANTC 2 cut(s) 69, 196
HphI GGTGA 1 cut(s) 437
Hpy188I TCNGA 3 cut(s) 178, 237, 390
Hpy188III TCNNGA 3 cut(s) 29, 73, 401
HpyCH4III ACNGT 1 cut(s) 208
HpyCH4IV ACGT 1 cut(s) 60
HpyCH4V TGCA 5 cut(s) 56, 130, 140, 286, 370
HpyF10VI GCNNNNNNNGC 2 cut(s) 239, 269
HpyF3I CTNAG 4 cut(s) 144, 152, 236, 387
HpySE526I ACGT 1 cut(s) 60
Hsp92II CATG 4 cut(s) 22, 76, 362, 453
Ksp22I TGATCA 1 cut(s) 25
Kzo9I GATC 2 cut(s) 4, 25
LmnI GCTCC 2 cut(s) 51, 379
LpnPI CCDG 6 cut(s) 14, 150, 251, 278, 380, 386
Lsp1109I GCAGC 3 cut(s) 256, 304, 307
LweI GCATC 1 cut(s) 33
MaeI CTAG 1 cut(s) 245
MaeII ACGT 1 cut(s) 60
MaeIII GTNAC 3 cut(s) 160, 185, 425
MalI GATC 2 cut(s) 6, 27
MboI GATC 2 cut(s) 4, 25
MboII GAAGA 1 cut(s) 77
MfeI CAATTG 1 cut(s) 51
MhlI GDGCHC 2 cut(s) 235, 244
MluCI AATT 3 cut(s) 51, 81, 102
MnlI CCTC 4 cut(s) 106, 163, 270, 356
MspR9I CCNGG 1 cut(s) 266
MunI CAATTG 1 cut(s) 51
MvaI CCWGG 1 cut(s) 266
MwoI GCNNNNNNNGC 2 cut(s) 239, 269
NdeII GATC 2 cut(s) 4, 25
NlaIII CATG 4 cut(s) 22, 76, 362, 453
NmuCI GTSAC 3 cut(s) 160, 185, 425
PagI TCATGA 1 cut(s) 72
PceI AGGCCT 1 cut(s) 366
PfeI GAWTC 2 cut(s) 69, 196
PkrI GCNGC 3 cut(s) 271, 319, 322
PshAI GACNNNNGTC 1 cut(s) 183
Psp6I CCWGG 1 cut(s) 264
PspGI CCWGG 1 cut(s) 264
RsaI GTAC 1 cut(s) 281
RsaNI GTAC 1 cut(s) 280
SatI GCNGC 3 cut(s) 270, 318, 321
Sau3AI GATC 2 cut(s) 4, 25
ScrFI CCNGG 1 cut(s) 266
SduI GDGCHC 2 cut(s) 235, 244
SetI ASST 4 cut(s) 63, 281, 319, 427
SfaNI GCATC 1 cut(s) 33
SmlI CTYRAG 1 cut(s) 416
SmoI CTYRAG 1 cut(s) 416
Sse9I AATT 3 cut(s) 51, 81, 102
SseBI AGGCCT 1 cut(s) 366
SspI AATATT 1 cut(s) 349
SspMI CTAG 1 cut(s) 245
StuI AGGCCT 1 cut(s) 366
StyD4I CCNGG 1 cut(s) 264
StyI CCWWGG 1 cut(s) 108
TaaI ACNGT 1 cut(s) 208
TaiI ACGT 1 cut(s) 63
TaqI TCGA 1 cut(s) 67
TasI AATT 3 cut(s) 51, 81, 102
TfiI GAWTC 2 cut(s) 69, 196
TseFI GTSAC 3 cut(s) 160, 185, 425
TseI GCWGC 3 cut(s) 269, 317, 320
Tsp45I GTSAC 3 cut(s) 160, 185, 425
TspDTI ATGAA 1 cut(s) 89
XapI RAATTY 2 cut(s) 81, 102
XspI CTAG 1 cut(s) 245
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.