MD12G1217100.v1.1

Nuclear transcription factor Y subunit

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr12
OG0015296
Physical Location & Seq
Forward (+)
29460289 .. 29460741
453 bp
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UTR
Exon/CDS
Intron
MD12G1217100.v1.1.491

Sequence Viewer

Length: 453 bp
ATGGATCGATCAATCAACATGGTAGATCAGGAACAAGATGGAATGCTCCCAATTGCAAACGTGGGTCGAATCATGAAGCAAATTCTTCCCCCAAGAGCCAAAATTTCCAAAGAAGCCAAACAAACAATGCAGGAATGTGCAACTGAGTTCCTAAGTTTTGTAACTGGCGAGGCTTCTGACAAGTGTCACAAGGAGAATCGCAAGACCGTCAATGGAGACGACATCTGTTGGGCTCTGAGTGCTCTAGGGTTTGATAACTATGCCCAGGCTACTATAAGGTACTTGCATAAATACAGGGAAGCTGAAAGAGACAAAGCTGCAGCTAACAACAACCAAAACACAGCAGATATTAAAAGTTATGGAGGCCTGCAGGTACCGCAGCAAAATCAAACTTCAAATCTGGAGTTTCGGGTGCTTGGGAAAGGTGACAACTCCCTTGTTACCAAGCCATGA

Protein Analysis

151

Amino Acids

16.79

Weight (kDa)

7.69

Isoelectric Point (pI)

30.64

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
CBFD_NFYB_HMF PF00808 16 - 79 1.6e-25 Histone-like transcription factor (CBF/NF-Y) and archaeal histone
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0015296)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G09030
fragaria_vesca FvH4_6g06220
malus_domestica MD04G1203300.v1.1 MD12G1217100.v1.1
prunus_persica Prupe.6G323400_v2.0.a1
pyrus_communis pycom04g17970
rosa_chinensis RchiOBHm_Chr3g0455241
rosa_laevigata RLG00000025374
rosa_multiflora Rmu_sc0002169.1_g000034
rosa_roxburghii Rroxscaffold_6G00424430
rosa_rugosa Rorug03G0005500
rosa_samantha Rh3AG065500 Rh3BG067600 Rh3CG066100 Rh3DG067700
rosa_wichuraiana Rw3G005160

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 361
Acc65I GGTACC 1 cut(s) 373
AccB1I GGYRCC 1 cut(s) 373
AciI CCGC 1 cut(s) 377
AclWI GGATC 1 cut(s) 12
AcsI RAATTY 2 cut(s) 81, 102
AfaI GTAC 2 cut(s) 281, 375
AgsI TTSAA 1 cut(s) 396
AjnI CCWGG 1 cut(s) 264
AluBI AGCT 3 cut(s) 302, 317, 323
AluI AGCT 3 cut(s) 302, 317, 323
Alw21I GWGCWC 1 cut(s) 244
Alw26I GTCTC 2 cut(s) 210, 303
AlwI GGATC 1 cut(s) 12
AoxI GGCC 1 cut(s) 364
ApeKI GCWGC 3 cut(s) 317, 320, 379
ApoI RAATTY 2 cut(s) 81, 102
Asp718I GGTACC 1 cut(s) 373
AsuHPI GGTGA 1 cut(s) 437
BanI GGYRCC 1 cut(s) 373
BanII GRGCYC 1 cut(s) 235
Bbv12I GWGCWC 1 cut(s) 244
BbvI GCAGC 3 cut(s) 304, 332, 391
BccI CCATC 1 cut(s) 32
BciT130I CCWGG 1 cut(s) 266
BcoDI GTCTC 2 cut(s) 210, 303
BfaI CTAG 1 cut(s) 245
BfmI CTRYAG 2 cut(s) 318, 368
BfuAI ACCTGC 1 cut(s) 361
BisI GCNGC 3 cut(s) 318, 321, 380
BlsI GCNGC 3 cut(s) 319, 322, 381
Bme1390I CCNGG 1 cut(s) 266
BmiI GGNNCC 1 cut(s) 375
BmrFI CCNGG 1 cut(s) 266
BoxI GACNNNNGTC 1 cut(s) 183
BpmI CTGGAG 1 cut(s) 422
Bsa29I ATCGAT 1 cut(s) 7
BsaJI CCNNGG 1 cut(s) 264
Bse1I ACTGG 1 cut(s) 169
BseBI CCWGG 1 cut(s) 266
BseCI ATCGAT 1 cut(s) 7
BseDI CCNNGG 1 cut(s) 264
BseMII CTCAG 2 cut(s) 135, 227
BseNI ACTGG 1 cut(s) 169
BseXI GCAGC 3 cut(s) 304, 332, 391
BshFI GGCC 1 cut(s) 366
BshNI GGYRCC 1 cut(s) 373
BshVI ATCGAT 1 cut(s) 7
BsiHKAI GWGCWC 1 cut(s) 244
BsmAI GTCTC 2 cut(s) 210, 303
BsmBI CGTCTC 1 cut(s) 210
BsmI GAATGC 1 cut(s) 48
BsnI GGCC 1 cut(s) 366
Bsp1286I GDGCHC 2 cut(s) 235, 244
Bsp143I GATC 3 cut(s) 4, 8, 25
BspACI CCGC 1 cut(s) 377
BspANI GGCC 1 cut(s) 366
BspCNI CTCAG 2 cut(s) 136, 228
BspDI ATCGAT 1 cut(s) 7
BspHI TCATGA 1 cut(s) 72
BspLI GGNNCC 1 cut(s) 375
BspMAI CTGCAG 2 cut(s) 322, 372
BspMI ACCTGC 1 cut(s) 361
BspPI GGATC 1 cut(s) 12
BspT107I GGYRCC 1 cut(s) 373
BsrI ACTGG 1 cut(s) 169
BssECI CCNNGG 1 cut(s) 264
BssMI GATC 3 cut(s) 4, 8, 25
Bst2UI CCWGG 1 cut(s) 266
Bst4CI ACNGT 1 cut(s) 208
BstC8I GCNNGC 1 cut(s) 368
BstDEI CTNAG 3 cut(s) 144, 152, 236
BstKTI GATC 3 cut(s) 7, 11, 28
BstMAI GTCTC 2 cut(s) 210, 303
BstMBI GATC 3 cut(s) 4, 8, 25
BstMWI GCNNNNNNNGC 2 cut(s) 239, 376
BstNI CCWGG 1 cut(s) 266
BstPAI GACNNNNGTC 1 cut(s) 183
BstSCI CCNGG 1 cut(s) 264
BstSFI CTRYAG 2 cut(s) 318, 368
BstV1I GCAGC 3 cut(s) 304, 332, 391
Bsu15I ATCGAT 1 cut(s) 7
BsuRI GGCC 1 cut(s) 366
BsuTUI ATCGAT 1 cut(s) 7
BveI ACCTGC 1 cut(s) 361
Cac8I GCNNGC 1 cut(s) 368
CciI TCATGA 1 cut(s) 72
ClaI ATCGAT 1 cut(s) 7
Csp6I GTAC 2 cut(s) 280, 374
CviAII CATG 3 cut(s) 19, 73, 450
CviQI GTAC 2 cut(s) 280, 374
DdeI CTNAG 3 cut(s) 144, 152, 236
DpnI GATC 3 cut(s) 6, 10, 27
DpnII GATC 3 cut(s) 4, 8, 25
Eco147I AGGCCT 1 cut(s) 366
Eco24I GRGCYC 1 cut(s) 235
EcoRII CCWGG 1 cut(s) 264
EcoT38I GRGCYC 1 cut(s) 235
Esp3I CGTCTC 1 cut(s) 210
FaeI CATG 3 cut(s) 22, 76, 453
FaiI YATR 7 cut(s) 20, 74, 261, 275, 288, 360, 451
FatI CATG 3 cut(s) 18, 72, 449
Fnu4HI GCNGC 3 cut(s) 318, 321, 380
FriOI GRGCYC 1 cut(s) 235
Fsp4HI GCNGC 3 cut(s) 318, 321, 380
FspBI CTAG 1 cut(s) 245
GluI GCNGC 3 cut(s) 318, 321, 380
GsuI CTGGAG 1 cut(s) 422
HaeIII GGCC 1 cut(s) 366
Hin1II CATG 3 cut(s) 22, 76, 453
HinfI GANTC 2 cut(s) 69, 196
HphI GGTGA 1 cut(s) 437
Hpy188I TCNGA 2 cut(s) 178, 237
Hpy188III TCNNGA 3 cut(s) 29, 73, 401
HpyCH4III ACNGT 1 cut(s) 208
HpyCH4IV ACGT 1 cut(s) 60
HpyCH4V TGCA 6 cut(s) 56, 130, 140, 286, 320, 370
HpyF10VI GCNNNNNNNGC 2 cut(s) 239, 376
HpyF3I CTNAG 3 cut(s) 144, 152, 236
HpySE526I ACGT 1 cut(s) 60
Hsp92II CATG 3 cut(s) 22, 76, 453
KpnI GGTACC 1 cut(s) 377
Kzo9I GATC 3 cut(s) 4, 8, 25
LmnI GCTCC 1 cut(s) 51
LpnPI CCDG 9 cut(s) 14, 116, 150, 251, 278, 280, 356, 380, 386
Lsp1109I GCAGC 3 cut(s) 304, 332, 391
MaeI CTAG 1 cut(s) 245
MaeII ACGT 1 cut(s) 60
MaeIII GTNAC 4 cut(s) 160, 185, 425, 439
MalI GATC 3 cut(s) 6, 10, 27
MboI GATC 3 cut(s) 4, 8, 25
MboII GAAGA 1 cut(s) 77
MfeI CAATTG 1 cut(s) 51
MhlI GDGCHC 2 cut(s) 235, 244
MluCI AATT 3 cut(s) 51, 81, 102
MnlI CCTC 2 cut(s) 163, 356
MseI TTAA 1 cut(s) 351
MspR9I CCNGG 1 cut(s) 266
MunI CAATTG 1 cut(s) 51
Mva1269I GAATGC 1 cut(s) 48
MvaI CCWGG 1 cut(s) 266
MwoI GCNNNNNNNGC 2 cut(s) 239, 376
NdeII GATC 3 cut(s) 4, 8, 25
NlaIII CATG 3 cut(s) 22, 76, 453
NlaIV GGNNCC 1 cut(s) 375
NmuCI GTSAC 2 cut(s) 185, 425
PagI TCATGA 1 cut(s) 72
PceI AGGCCT 1 cut(s) 366
PctI GAATGC 1 cut(s) 48
PfeI GAWTC 2 cut(s) 69, 196
PkrI GCNGC 3 cut(s) 319, 322, 381
PshAI GACNNNNGTC 1 cut(s) 183
Psp6I CCWGG 1 cut(s) 264
PspGI CCWGG 1 cut(s) 264
PspN4I GGNNCC 1 cut(s) 375
PstI CTGCAG 2 cut(s) 322, 372
RsaI GTAC 2 cut(s) 281, 375
RsaNI GTAC 2 cut(s) 280, 374
SaqAI TTAA 1 cut(s) 351
SatI GCNGC 3 cut(s) 318, 321, 380
Sau3AI GATC 3 cut(s) 4, 8, 25
SbfI CCTGCAGG 1 cut(s) 372
ScrFI CCNGG 1 cut(s) 266
SdaI CCTGCAGG 1 cut(s) 372
SduI GDGCHC 2 cut(s) 235, 244
SetI ASST 7 cut(s) 63, 281, 304, 319, 325, 375, 427
SfcI CTRYAG 2 cut(s) 318, 368
Sse8387I CCTGCAGG 1 cut(s) 372
Sse9I AATT 3 cut(s) 51, 81, 102
SseBI AGGCCT 1 cut(s) 366
SsiI CCGC 1 cut(s) 377
SspMI CTAG 1 cut(s) 245
StuI AGGCCT 1 cut(s) 366
StyD4I CCNGG 1 cut(s) 264
TaaI ACNGT 1 cut(s) 208
TaiI ACGT 1 cut(s) 63
TaqI TCGA 2 cut(s) 7, 67
TasI AATT 3 cut(s) 51, 81, 102
TfiI GAWTC 2 cut(s) 69, 196
Tru1I TTAA 1 cut(s) 351
Tru9I TTAA 1 cut(s) 351
TseFI GTSAC 2 cut(s) 185, 425
TseI GCWGC 3 cut(s) 317, 320, 379
Tsp45I GTSAC 2 cut(s) 185, 425
TspDTI ATGAA 1 cut(s) 89
XapI RAATTY 2 cut(s) 81, 102
XspI CTAG 1 cut(s) 245
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.