MD04G1219400.v1.1

Mediator-associated protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr04
OG0014757
Physical Location & Seq
Reverse (-)
30122720 .. 30123805
1086 bp
Loading structure...
UTR
Exon/CDS
Intron
MD04G1219400.v1.1.491

Sequence Viewer

Length: 1086 bp
ATGGCGTGGCGGCCGTTGCTTAGTTGGATCATGTCTTCTTCTTCCTCTTCTTCGTCTTCTTATTCATCCTCAGAGCATGAATCTAAGTCTGAGGATGACCTTCGCCATGTTGACCCCGGTAACAACAACGTCCAGCAAGCACAGAAGGGTCACAATGTTGATGATCATGACAACGAGGATGATGACAATGACGTTTCATACGAACCCCATGAGGACGAGGAAGATGACGAGGAGAAAGTTGAGGAACCAAAATCCAGCTCCACCTCCGACTACAGCTTCACAATATACGAGCCCCGTCTGAAACGACGCCGTATTGACCATGCAAATGAGGCCTTGACGTCCCTACAAGAGGTAAAGAAACGGCGGGGTAAGAGGGTTTGGTGCAAGGACGACGAGCTTGAGCTGTTGCGTGGCTTTCTTGACTACTACGGCACCAGACAAACCAAAATTACCAGAACCACCGCCAAGGAAACATCGTCGTTGTACGAAGAAATCAAGCCGAGGTTAAAGCTTGAATGCAACAAGACAAAGATGGTGGAGAAGCTGAGAAGACTAAAGAGGAAGTACCACAATGTCGTGAAGAAAACGAACGAAATCAACTCCTCCGGGAAAGAGTTTCGGTTCAAAAACGCACATGACAATGCAGTTTTCGAGCTTTCACATATGATTTGGAATGTCGAAAACAGAAATAATTGTGTCGTGGAATCGCATAATAATAACGTTATGGTGAAGAGGGAGGAGGACAATGTGGCGGCGAGTAGTAGCGTTGCGGATCATGAGAGGCCAACGACGTGGTTTGATGAGAATAATAATGTTACTAATGTGGATGATAACATCAATACTGGTCATGATGTGAGACGATTGATAGTAGGGTTGGCTATGAATCCAATGCCTCTGAGTTTGAAGGGTAAAATGGGAAGTGGCGGCGGCGGTGGCGGTGGTGGGGTTGGAGAAGTGGTGGAGGATCAGAAGTGGAGGCAGCAGCAGATTTTGGAGTTGGAGTCTTATGAAAAGAGGTTGGATTTGGTGCAGCATCAGGTTAAGGCAGCTTTGGAGGAATTGAGGACCAAGGGAGGAGCTCACTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF573

Protein Analysis

362

Amino Acids

41.4

Weight (kDa)

5.76

Isoelectric Point (pI)

55.32

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
GeBP-like_DBD PF04504 124 - 225 1.7e-23 Glabrous-enhancer-binding protein-like family, DBD domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 341
AccB1I GGYRCC 1 cut(s) 431
AciI CCGC 9 cut(s) 10, 364, 462, 752, 770, 924, 927, 930, 936
AclI AACGTT 1 cut(s) 720
AclWI GGATC 3 cut(s) 35, 780, 972
AcoI YGGCCR 1 cut(s) 11
AcyI GRCGYC 2 cut(s) 307, 338
AfaI GTAC 2 cut(s) 485, 566
AfiI CCNNNNNNNGG 1 cut(s) 349
AgsI TTSAA 3 cut(s) 515, 625, 904
AjiI CACGTC 1 cut(s) 792
AluBI AGCT 9 cut(s) 258, 276, 397, 403, 511, 544, 655, 1049, 1079
AluI AGCT 9 cut(s) 258, 276, 397, 403, 511, 544, 655, 1049, 1079
Alw21I GWGCWC 1 cut(s) 1081
Alw26I GTCTC 1 cut(s) 850
AlwI GGATC 3 cut(s) 35, 780, 972
AoxI GGCC 3 cut(s) 11, 330, 782
ApeKI GCWGC 4 cut(s) 979, 982, 1030, 1046
AspS9I GGNCC 1 cut(s) 1065
AsuC2I CCSGG 2 cut(s) 117, 607
AsuHPI GGTGA 1 cut(s) 739
AvaII GGWCC 1 cut(s) 1065
BanI GGYRCC 1 cut(s) 431
BanII GRGCYC 2 cut(s) 294, 1081
BbsI GAAGAC 3 cut(s) 27, 48, 556
Bbv12I GWGCWC 1 cut(s) 1081
BbvI GCAGC 4 cut(s) 991, 994, 1042, 1058
BccI CCATC 1 cut(s) 526
BceAI ACGGC 3 cut(s) 294, 377, 445
BclI TGATCA 1 cut(s) 163
BcnI CCSGG 2 cut(s) 117, 607
BcoDI GTCTC 1 cut(s) 850
BfmI CTRYAG 1 cut(s) 271
BisI GCNGC 8 cut(s) 11, 753, 925, 928, 980, 983, 1031, 1047
BlsI GCNGC 8 cut(s) 12, 754, 926, 929, 981, 984, 1032, 1048
Bme1390I CCNGG 2 cut(s) 117, 607
Bme18I GGWCC 1 cut(s) 1065
BmgBI CACGTC 1 cut(s) 792
BmgT120I GGNCC 1 cut(s) 1065
BmiI GGNNCC 2 cut(s) 246, 433
BmrFI CCNGG 2 cut(s) 117, 607
BmsI GCATC 1 cut(s) 1042
BpiI GAAGAC 3 cut(s) 27, 48, 556
BpuEI CTTGAG 1 cut(s) 419
BpuMI CCSGG 2 cut(s) 117, 607
BsaHI GRCGYC 2 cut(s) 307, 338
BsaJI CCNNGG 4 cut(s) 115, 465, 500, 1068
BsaXI ACNNNNNCTCC 4 cut(s) 942, 972, 986, 1016
Bsc4I CCNNNNNNNGG 1 cut(s) 349
Bse1I ACTGG 1 cut(s) 847
BseDI CCNNGG 4 cut(s) 115, 465, 500, 1068
BseGI GGATG 4 cut(s) 65, 100, 184, 832
BseLI CCNNNNNNNGG 1 cut(s) 349
BseMII CTCAG 4 cut(s) 81, 84, 536, 887
BseNI ACTGG 1 cut(s) 847
BseRI GAGGAG 3 cut(s) 245, 592, 752
BseX3I CGGCCG 1 cut(s) 11
BseXI GCAGC 4 cut(s) 991, 994, 1042, 1058
BsgI GTGCAG 1 cut(s) 1049
Bsh1285I CGRYCG 1 cut(s) 14
BshFI GGCC 3 cut(s) 13, 332, 784
BshNI GGYRCC 1 cut(s) 431
BsiEI CGRYCG 1 cut(s) 14
BsiHKAI GWGCWC 1 cut(s) 1081
BsiSI CCGG 2 cut(s) 117, 606
BslFI GGGAC 1 cut(s) 325
BslI CCNNNNNNNGG 1 cut(s) 349
BsmAI GTCTC 1 cut(s) 850
BsmBI CGTCTC 1 cut(s) 850
BsmFI GGGAC 1 cut(s) 325
BsmI GAATGC 1 cut(s) 521
BsnI GGCC 3 cut(s) 13, 332, 784
Bsp1286I GDGCHC 2 cut(s) 294, 1081
Bsp143I GATC 4 cut(s) 27, 163, 772, 964
BspACI CCGC 9 cut(s) 10, 364, 462, 752, 770, 924, 927, 930, 936
BspANI GGCC 3 cut(s) 13, 332, 784
BspCNI CTCAG 4 cut(s) 82, 83, 537, 888
BspHI TCATGA 3 cut(s) 166, 775, 847
BspLI GGNNCC 2 cut(s) 246, 433
BspPI GGATC 3 cut(s) 35, 780, 972
BspT107I GGYRCC 1 cut(s) 431
BsrI ACTGG 1 cut(s) 847
BssECI CCNNGG 4 cut(s) 115, 465, 500, 1068
BssMI GATC 4 cut(s) 27, 163, 772, 964
BssNI GRCGYC 2 cut(s) 307, 338
BssT1I CCWWGG 2 cut(s) 465, 1068
Bst6I CTCTTC 2 cut(s) 52, 725
BstACI GRCGYC 2 cut(s) 307, 338
BstC8I GCNNGC 1 cut(s) 138
BstDEI CTNAG 6 cut(s) 20, 70, 84, 90, 545, 896
BstENI CCTNNNNNAGG 1 cut(s) 347
BstF5I GGATG 4 cut(s) 65, 100, 184, 832
BstKTI GATC 4 cut(s) 30, 166, 775, 967
BstMAI GTCTC 1 cut(s) 850
BstMBI GATC 4 cut(s) 27, 163, 772, 964
BstMCI CGRYCG 1 cut(s) 14
BstMWI GCNNNNNNNGC 3 cut(s) 16, 329, 933
BstSCI CCNGG 2 cut(s) 115, 605
BstSFI CTRYAG 1 cut(s) 271
BstV1I GCAGC 4 cut(s) 991, 994, 1042, 1058
BstV2I GAAGAC 3 cut(s) 27, 48, 556
BstXI CCANNNNNNTGG 1 cut(s) 792
BstZI CGGCCG 1 cut(s) 11
BsuRI GGCC 3 cut(s) 13, 332, 784
BtrI CACGTC 1 cut(s) 792
BtsCI GGATG 4 cut(s) 65, 100, 184, 832
Cac8I GCNNGC 1 cut(s) 138
CciI TCATGA 3 cut(s) 166, 775, 847
Cfr13I GGNCC 1 cut(s) 1065
CseI GACGC 1 cut(s) 315
Csp6I GTAC 2 cut(s) 484, 565
CspCI CAANNNNNGTGG 2 cut(s) 516, 551
CviAII CATG 9 cut(s) 31, 77, 107, 167, 209, 320, 635, 776, 848
CviQI GTAC 2 cut(s) 484, 565
DdeI CTNAG 6 cut(s) 20, 70, 84, 90, 545, 896
DpnI GATC 4 cut(s) 29, 165, 774, 966
DpnII GATC 4 cut(s) 27, 163, 772, 964
EaeI YGGCCR 1 cut(s) 11
EagI CGGCCG 1 cut(s) 11
Eam1104I CTCTTC 2 cut(s) 52, 725
EarI CTCTTC 2 cut(s) 52, 725
Ecl136II GAGCTC 1 cut(s) 1079
EclXI CGGCCG 1 cut(s) 11
Eco130I CCWWGG 2 cut(s) 465, 1068
Eco147I AGGCCT 1 cut(s) 332
Eco24I GRGCYC 2 cut(s) 294, 1081
Eco47I GGWCC 1 cut(s) 1065
Eco52I CGGCCG 1 cut(s) 11
Eco53kI GAGCTC 1 cut(s) 1079
EcoICRI GAGCTC 1 cut(s) 1079
EcoNI CCTNNNNNAGG 1 cut(s) 347
EcoT14I CCWWGG 2 cut(s) 465, 1068
EcoT38I GRGCYC 2 cut(s) 294, 1081
ErhI CCWWGG 2 cut(s) 465, 1068
Esp3I CGTCTC 1 cut(s) 850
FaeI CATG 9 cut(s) 34, 80, 110, 170, 212, 323, 638, 779, 851
FaqI GGGAC 1 cut(s) 325
FatI CATG 9 cut(s) 30, 76, 106, 166, 208, 319, 634, 775, 847
FauI CCCGC 1 cut(s) 357
FauNDI CATATG 1 cut(s) 663
FbaI TGATCA 1 cut(s) 163
Fnu4HI GCNGC 8 cut(s) 11, 753, 925, 928, 980, 983, 1031, 1047
FokI GGATG 4 cut(s) 52, 107, 191, 839
FriOI GRGCYC 2 cut(s) 294, 1081
Fsp4HI GCNGC 8 cut(s) 11, 753, 925, 928, 980, 983, 1031, 1047
GluI GCNGC 8 cut(s) 11, 753, 925, 928, 980, 983, 1031, 1047
HaeIII GGCC 3 cut(s) 13, 332, 784
HapII CCGG 2 cut(s) 117, 606
HgaI GACGC 1 cut(s) 315
Hin1I GRCGYC 2 cut(s) 307, 338
Hin1II CATG 9 cut(s) 34, 80, 110, 170, 212, 323, 638, 779, 851
HincII GTYRAC 1 cut(s) 112
HindII GTYRAC 1 cut(s) 112
HindIII AAGCTT 1 cut(s) 509
HinfI GANTC 4 cut(s) 80, 704, 883, 1001
HpaII CCGG 2 cut(s) 117, 606
HphI GGTGA 1 cut(s) 739
Hpy166II GTNNAC 1 cut(s) 112
Hpy188I TCNGA 6 cut(s) 73, 91, 268, 300, 897, 969
Hpy188III TCNNGA 5 cut(s) 167, 419, 577, 776, 848
Hpy8I GTNNAC 1 cut(s) 112
Hpy99I CGWCG 4 cut(s) 309, 395, 481, 793
HpyAV CCTTC 3 cut(s) 110, 139, 898
HpyCH4IV ACGT 5 cut(s) 129, 192, 338, 720, 791
HpyCH4V TGCA 5 cut(s) 323, 384, 519, 644, 1030
HpyF10VI GCNNNNNNNGC 3 cut(s) 16, 329, 933
HpyF3I CTNAG 6 cut(s) 20, 70, 84, 90, 545, 896
HpySE526I ACGT 5 cut(s) 129, 192, 338, 720, 791
Hsp92I GRCGYC 2 cut(s) 307, 338
Hsp92II CATG 9 cut(s) 34, 80, 110, 170, 212, 323, 638, 779, 851
Ksp22I TGATCA 1 cut(s) 163
Kzo9I GATC 4 cut(s) 27, 163, 772, 964
LmnI GCTCC 2 cut(s) 263, 1076
LpnPI CCDG 8 cut(s) 130, 146, 268, 448, 466, 619, 828, 1022
Lsp1109I GCAGC 4 cut(s) 991, 994, 1042, 1058
LweI GCATC 1 cut(s) 1042
MaeII ACGT 5 cut(s) 129, 192, 338, 720, 791
MaeIII GTNAC 3 cut(s) 119, 149, 814
MalI GATC 4 cut(s) 29, 165, 774, 966
MboI GATC 4 cut(s) 27, 163, 772, 964
MhlI GDGCHC 2 cut(s) 294, 1081
MluCI AATT 3 cut(s) 447, 691, 1058
MlyI GAGTC 1 cut(s) 1010
MmeI TCCRAC 5 cut(s) 5, 291, 928, 978, 999
MseI TTAA 2 cut(s) 506, 1041
MslI CAYNNNNRTG 2 cut(s) 324, 639
MspI CCGG 2 cut(s) 117, 606
MspR9I CCNGG 2 cut(s) 117, 607
Mva1269I GAATGC 1 cut(s) 521
MwoI GCNNNNNNNGC 3 cut(s) 16, 329, 933
NciI CCSGG 2 cut(s) 117, 607
NdeI CATATG 1 cut(s) 663
NdeII GATC 4 cut(s) 27, 163, 772, 964
NlaIII CATG 9 cut(s) 34, 80, 110, 170, 212, 323, 638, 779, 851
NlaIV GGNNCC 2 cut(s) 246, 433
NmeAIII GCCGAG 1 cut(s) 525
NmuCI GTSAC 1 cut(s) 149
PagI TCATGA 3 cut(s) 166, 775, 847
PceI AGGCCT 1 cut(s) 332
PcsI WCGNNNNNNNCGW 1 cut(s) 198
PctI GAATGC 1 cut(s) 521
PfeI GAWTC 3 cut(s) 80, 704, 883
PfoI TCCNGGA 1 cut(s) 605
PkrI GCNGC 8 cut(s) 12, 754, 926, 929, 981, 984, 1032, 1048
PleI GAGTC 1 cut(s) 1009
PpsI GAGTC 1 cut(s) 1009
Psp124BI GAGCTC 1 cut(s) 1081
Psp1406I AACGTT 1 cut(s) 720
PspN4I GGNNCC 2 cut(s) 246, 433
PspPI GGNCC 1 cut(s) 1065
RsaI GTAC 2 cut(s) 485, 566
RsaNI GTAC 2 cut(s) 484, 565
RseI CAYNNNNRTG 2 cut(s) 324, 639
SacI GAGCTC 1 cut(s) 1081
SaqAI TTAA 2 cut(s) 506, 1041
SatI GCNGC 8 cut(s) 11, 753, 925, 928, 980, 983, 1031, 1047
Sau3AI GATC 4 cut(s) 27, 163, 772, 964
Sau96I GGNCC 1 cut(s) 1065
SchI GAGTC 1 cut(s) 1010
ScrFI CCNGG 2 cut(s) 117, 607
SduI GDGCHC 2 cut(s) 294, 1081
SfaNI GCATC 1 cut(s) 1042
SfcI CTRYAG 1 cut(s) 271
SinI GGWCC 1 cut(s) 1065
SmiMI CAYNNNNRTG 2 cut(s) 324, 639
SmlI CTYRAG 1 cut(s) 398
SmoI CTYRAG 1 cut(s) 398
Sse9I AATT 3 cut(s) 447, 691, 1058
SseBI AGGCCT 1 cut(s) 332
SsiI CCGC 9 cut(s) 10, 364, 462, 752, 770, 924, 927, 930, 936
SstI GAGCTC 1 cut(s) 1081
StuI AGGCCT 1 cut(s) 332
StyD4I CCNGG 2 cut(s) 115, 605
StyI CCWWGG 2 cut(s) 465, 1068
TaiI ACGT 5 cut(s) 132, 195, 341, 723, 794
TaqI TCGA 2 cut(s) 651, 678
TasI AATT 3 cut(s) 447, 691, 1058
TauI GCSGC 4 cut(s) 13, 755, 927, 930
TfiI GAWTC 3 cut(s) 80, 704, 883
Tru1I TTAA 2 cut(s) 506, 1041
Tru9I TTAA 2 cut(s) 506, 1041
TseFI GTSAC 1 cut(s) 149
TseI GCWGC 4 cut(s) 979, 982, 1030, 1046
Tsp45I GTSAC 1 cut(s) 149
TspDTI ATGAA 5 cut(s) 54, 93, 186, 896, 1023
VpaK11BI GGWCC 1 cut(s) 1065
XagI CCTNNNNNAGG 1 cut(s) 347
ZraI GACGTC 1 cut(s) 339
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.