Prupe.6G338700_v2.0.a1

Mediator-associated protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
prunus_persica
Pp06
OG0014757
Physical Location & Seq
Reverse (-)
29357338 .. 29358459
1122 bp
Loading structure...
UTR
Exon/CDS
Intron
Prupe.6G338700.1

Sequence Viewer

Length: 1122 bp
ATGGCATGGCCGCCATTGCTTAGTTGGATCCTATCTTCTTCTTCTTCTTCGTCTTCCTCAGAGTATGAATCCGACGACAACAACGACATTGAAGATTTAGGAGGAATTAATGATGTCCCCGACAACAACCCAAAATCACAAAATGACAATATTGATGATCATGAAAACGACGACGTCTCATACCAACCGCAGGAGGAGGATCAAAACTCCAACTCCACCTCCGACTACACCTTCACAGTCTCCGAACCCAGCGCAAAACGACGACGCATTGATCGCACATACAAGCCAGCATTGACGTCCGATGCACCCGAGGAAAACAAGAAACGGCGTCGAAAAAGGGTGTGGAGCAAGGAGGACGAGCTGGAGTTGTTGCGGAGTTTGCTCGACGACTACTACAGCAGGGGACAAATTAGAACCACCGTGAAGGACACGGCGTCGTTTTACGAAGAGGTTAAGCCCAAGTTAAAGCTGGAATGTAACAGGGCCCAAATGGTCGAGAAGCTGAGGAGATTGAAGAAGAAGTACCACAACGTCGTAAACAAAATGAAGGACATGAACTCGACTGGCAAAGAGTTCCGCTTCAAAAACCCGCACGACAAGGTCGTTTTTGAACTTTCCGACAAGATTTGGAACGTCGTAGATTCGCATGAAGATTATGCACCAAATTCTAAGTACGTTAGTATTTGCAACAATGTTAAGGTGAAAAAGGAGGAGGACAACGTGGGTAGCAGCAGCGTTGCAGATCATGAACGGCCACAGATGAGACACACGTGGTCGCATTCTAGGCTAACAACAACGTTTGATGAGAACACTAAGGCTCATGATGTTTTGAATGATAATGGAACGACGTCGTCGTTGAATAATAATAATAAAAATAAAAATAATAACAATAATGTGGATGATAATATGAAGAATGATATACCAGGGTTGATAATTGAGGGGCTGGCTATGAATCCATTACCTCTGAGTTTAAGTAAATTTGGAAGTGGGGTTGAGGTGGCAGATGAAAAGTGGAGGAAGCAGCAGATTTTGGAGTTGGAGACATACTCTAAAAGGTTGGATTTGGTGCAGCATCAGGTTAAGGCAGCTTTGGATGAATTGCGGTCCAAGGGAGATGAGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF573

Protein Analysis

374

Amino Acids

42.96

Weight (kDa)

5.55

Isoelectric Point (pI)

53.28

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 3 cut(s) 177, 299, 851
AciI CCGC 6 cut(s) 11, 188, 373, 577, 590, 1102
AclI AACGTT 1 cut(s) 797
AclWI GGATC 3 cut(s) 22, 35, 207
AcoI YGGCCR 2 cut(s) 8, 752
AcsI RAATTY 2 cut(s) 664, 977
AcvI CACGTG 1 cut(s) 771
AcyI GRCGYC 5 cut(s) 174, 296, 328, 434, 848
AfaI GTAC 2 cut(s) 524, 674
AfiI CCNNNNNNNGG 1 cut(s) 190
AflIII ACRYGT 1 cut(s) 768
AgsI TTSAA 6 cut(s) 92, 514, 583, 611, 832, 859
AhdI GACNNNNNGTC 1 cut(s) 433
AjnI CCWGG 1 cut(s) 922
AluBI AGCT 4 cut(s) 361, 469, 502, 1088
AluI AGCT 4 cut(s) 361, 469, 502, 1088
Alw26I GTCTC 4 cut(s) 181, 244, 757, 1034
AlwI GGATC 3 cut(s) 22, 35, 207
Ama87I CYCGRG 1 cut(s) 308
AoxI GGCC 3 cut(s) 8, 483, 752
ApaI GGGCCC 1 cut(s) 487
ApeKI GCWGC 5 cut(s) 729, 732, 1021, 1069, 1085
ApoI RAATTY 2 cut(s) 664, 977
AseI ATTAAT 1 cut(s) 108
AspLEI GCGC 1 cut(s) 254
AspS9I GGNCC 3 cut(s) 483, 484, 1104
AsuHPI GGTGA 1 cut(s) 712
AvaI CYCGRG 1 cut(s) 308
AvaII GGWCC 1 cut(s) 1104
BaeGI GKGCMC 1 cut(s) 487
BamHI GGATCC 1 cut(s) 27
BanII GRGCYC 1 cut(s) 487
BarI GAAGNNNNNNTAC 2 cut(s) 506, 538
BbrPI CACGTG 1 cut(s) 771
BbsI GAAGAC 1 cut(s) 45
BbvCI CCTCAGC 1 cut(s) 503
BbvI GCAGC 5 cut(s) 741, 744, 1033, 1081, 1097
BceAI ACGGC 3 cut(s) 341, 447, 767
BciT130I CCWGG 1 cut(s) 924
BclI TGATCA 1 cut(s) 157
BcoDI GTCTC 4 cut(s) 181, 244, 757, 1034
BfaI CTAG 1 cut(s) 783
BfmI CTRYAG 1 cut(s) 394
BisI GCNGC 6 cut(s) 11, 730, 733, 1022, 1070, 1086
BlsI GCNGC 6 cut(s) 12, 731, 734, 1023, 1071, 1087
Bme1390I CCNGG 1 cut(s) 924
Bme18I GGWCC 1 cut(s) 1104
BmeRI GACNNNNNGTC 1 cut(s) 433
BmeT110I CYCGRG 1 cut(s) 308
BmgT120I GGNCC 3 cut(s) 483, 484, 1104
BmiI GGNNCC 2 cut(s) 29, 485
BmrFI CCNGG 1 cut(s) 924
BmsI GCATC 2 cut(s) 292, 1081
BpiI GAAGAC 1 cut(s) 45
BplI GAGNNNNNCTC 2 cut(s) 1031, 1063
BpmI CTGGAG 1 cut(s) 383
Bpu10I CCTNAGC 1 cut(s) 503
BsaAI YACGTR 1 cut(s) 771
BsaHI GRCGYC 5 cut(s) 174, 296, 328, 434, 848
BsaJI CCNNGG 3 cut(s) 309, 923, 1107
BsaXI ACNNNNNCTCC 4 cut(s) 197, 203, 227, 233
Bsc4I CCNNNNNNNGG 1 cut(s) 190
Bse1I ACTGG 1 cut(s) 568
Bse3DI GCAATG 1 cut(s) 14
BseBI CCWGG 1 cut(s) 924
BseDI CCNNGG 3 cut(s) 309, 923, 1107
BseGI GGATG 2 cut(s) 904, 1099
BseLI CCNNNNNNNGG 1 cut(s) 190
BseMI GCAATG 1 cut(s) 14
BseMII CTCAG 3 cut(s) 72, 494, 956
BseNI ACTGG 1 cut(s) 568
BseRI GAGGAG 3 cut(s) 209, 520, 725
BseSI GKGCMC 1 cut(s) 487
BseXI GCAGC 5 cut(s) 741, 744, 1033, 1081, 1097
BseYI CCCAGC 1 cut(s) 248
BsgI GTGCAG 1 cut(s) 1088
BshFI GGCC 3 cut(s) 10, 485, 754
BsiHKCI CYCGRG 1 cut(s) 308
BslFI GGGAC 2 cut(s) 101, 417
BslI CCNNNNNNNGG 1 cut(s) 190
BsmAI GTCTC 4 cut(s) 181, 244, 757, 1034
BsmBI CGTCTC 1 cut(s) 181
BsmFI GGGAC 2 cut(s) 101, 417
BsmI GAATGC 1 cut(s) 778
BsnI GGCC 3 cut(s) 10, 485, 754
BsoBI CYCGRG 1 cut(s) 308
Bsp120I GGGCCC 1 cut(s) 483
Bsp1286I GDGCHC 1 cut(s) 487
Bsp143I GATC 5 cut(s) 27, 157, 199, 271, 742
BspACI CCGC 6 cut(s) 11, 188, 373, 577, 590, 1102
BspANI GGCC 3 cut(s) 10, 485, 754
BspCNI CTCAG 3 cut(s) 71, 495, 957
BspHI TCATGA 3 cut(s) 160, 745, 820
BspLI GGNNCC 2 cut(s) 29, 485
BspPI GGATC 3 cut(s) 22, 35, 207
BsrDI GCAATG 1 cut(s) 14
BsrI ACTGG 1 cut(s) 568
BssECI CCNNGG 3 cut(s) 309, 923, 1107
BssMI GATC 5 cut(s) 27, 157, 199, 271, 742
BssNI GRCGYC 5 cut(s) 174, 296, 328, 434, 848
BssT1I CCWWGG 1 cut(s) 1107
Bst2UI CCWGG 1 cut(s) 924
Bst4CI ACNGT 2 cut(s) 238, 421
Bst6I CTCTTC 1 cut(s) 441
BstACI GRCGYC 5 cut(s) 174, 296, 328, 434, 848
BstBAI YACGTR 1 cut(s) 771
BstC8I GCNNGC 2 cut(s) 288, 945
BstDEI CTNAG 6 cut(s) 20, 58, 503, 669, 813, 965
BstF5I GGATG 2 cut(s) 904, 1099
BstHHI GCGC 1 cut(s) 254
BstKTI GATC 5 cut(s) 30, 160, 202, 274, 745
BstMAI GTCTC 4 cut(s) 181, 244, 757, 1034
BstMBI GATC 5 cut(s) 27, 157, 199, 271, 742
BstMWI GCNNNNNNNGC 4 cut(s) 16, 273, 379, 784
BstNI CCWGG 1 cut(s) 924
BstSCI CCNGG 1 cut(s) 922
BstSFI CTRYAG 1 cut(s) 394
BstSLI GKGCMC 1 cut(s) 487
BstV1I GCAGC 5 cut(s) 741, 744, 1033, 1081, 1097
BstV2I GAAGAC 1 cut(s) 45
BstX2I RGATCY 1 cut(s) 27
BstYI RGATCY 1 cut(s) 27
BsuRI GGCC 3 cut(s) 10, 485, 754
BtsCI GGATG 2 cut(s) 904, 1099
Cac8I GCNNGC 2 cut(s) 288, 945
CciI TCATGA 3 cut(s) 160, 745, 820
CfoI GCGC 1 cut(s) 254
Cfr13I GGNCC 3 cut(s) 483, 484, 1104
CseI GACGC 3 cut(s) 273, 317, 423
Csp6I GTAC 2 cut(s) 523, 673
CviAII CATG 6 cut(s) 6, 161, 553, 647, 746, 821
CviQI GTAC 2 cut(s) 523, 673
DdeI CTNAG 6 cut(s) 20, 58, 503, 669, 813, 965
DpnI GATC 5 cut(s) 29, 159, 201, 273, 744
DpnII GATC 5 cut(s) 27, 157, 199, 271, 742
DriI GACNNNNNGTC 1 cut(s) 433
EaeI YGGCCR 2 cut(s) 8, 752
Eam1104I CTCTTC 1 cut(s) 441
Eam1105I GACNNNNNGTC 1 cut(s) 433
EarI CTCTTC 1 cut(s) 441
Eco130I CCWWGG 1 cut(s) 1107
Eco24I GRGCYC 1 cut(s) 487
Eco47I GGWCC 1 cut(s) 1104
Eco72I CACGTG 1 cut(s) 771
Eco88I CYCGRG 1 cut(s) 308
EcoO109I RGGNCCY 1 cut(s) 483
EcoRII CCWGG 1 cut(s) 922
EcoT14I CCWWGG 1 cut(s) 1107
EcoT38I GRGCYC 1 cut(s) 487
ErhI CCWWGG 1 cut(s) 1107
Esp3I CGTCTC 1 cut(s) 181
FaeI CATG 6 cut(s) 9, 164, 556, 650, 749, 824
FaqI GGGAC 2 cut(s) 101, 417
FatI CATG 6 cut(s) 5, 160, 552, 646, 745, 820
FauI CCCGC 1 cut(s) 597
FbaI TGATCA 1 cut(s) 157
Fnu4HI GCNGC 6 cut(s) 11, 730, 733, 1022, 1070, 1086
FokI GGATG 2 cut(s) 911, 1106
FriOI GRGCYC 1 cut(s) 487
Fsp4HI GCNGC 6 cut(s) 11, 730, 733, 1022, 1070, 1086
FspBI CTAG 1 cut(s) 783
GlaI GCGC 1 cut(s) 253
GluI GCNGC 6 cut(s) 11, 730, 733, 1022, 1070, 1086
GsaI CCCAGC 1 cut(s) 252
GsuI CTGGAG 1 cut(s) 383
HaeIII GGCC 3 cut(s) 10, 485, 754
HgaI GACGC 3 cut(s) 273, 317, 423
HhaI GCGC 1 cut(s) 254
Hin1I GRCGYC 5 cut(s) 174, 296, 328, 434, 848
Hin1II CATG 6 cut(s) 9, 164, 556, 650, 749, 824
Hin6I GCGC 1 cut(s) 252
HinP1I GCGC 1 cut(s) 252
HinfI GANTC 3 cut(s) 68, 641, 952
HphI GGTGA 1 cut(s) 712
Hpy166II GTNNAC 1 cut(s) 538
Hpy188I TCNGA 7 cut(s) 61, 73, 223, 244, 301, 619, 966
Hpy188III TCNNGA 4 cut(s) 161, 496, 746, 821
Hpy8I GTNNAC 1 cut(s) 538
HpyAV CCTTC 3 cut(s) 241, 418, 541
HpyCH4III ACNGT 2 cut(s) 238, 421
HpyCH4IV ACGT 9 cut(s) 174, 296, 531, 633, 675, 720, 770, 797, 848
HpyCH4V TGCA 5 cut(s) 305, 659, 687, 740, 1069
HpyF10VI GCNNNNNNNGC 4 cut(s) 16, 273, 379, 784
HpyF3I CTNAG 6 cut(s) 20, 58, 503, 669, 813, 965
HpySE526I ACGT 9 cut(s) 174, 296, 531, 633, 675, 720, 770, 797, 848
Hsp92I GRCGYC 5 cut(s) 174, 296, 328, 434, 848
Hsp92II CATG 6 cut(s) 9, 164, 556, 650, 749, 824
HspAI GCGC 1 cut(s) 252
Ksp22I TGATCA 1 cut(s) 157
Kzo9I GATC 5 cut(s) 27, 157, 199, 271, 742
LmnI GCTCC 1 cut(s) 345
Lsp1109I GCAGC 5 cut(s) 741, 744, 1033, 1081, 1097
LweI GCATC 2 cut(s) 292, 1081
MaeI CTAG 1 cut(s) 783
MaeII ACGT 9 cut(s) 174, 296, 531, 633, 675, 720, 770, 797, 848
MaeIII GTNAC 1 cut(s) 476
MalI GATC 5 cut(s) 29, 159, 201, 273, 744
MboI GATC 5 cut(s) 27, 157, 199, 271, 742
MflI RGATCY 1 cut(s) 27
MhlI GDGCHC 1 cut(s) 487
MluCI AATT 6 cut(s) 105, 408, 664, 933, 977, 1097
MmeI TCCRAC 7 cut(s) 5, 96, 234, 246, 642, 1017, 1038
MseI TTAA 6 cut(s) 108, 453, 464, 696, 971, 1080
MspR9I CCNGG 1 cut(s) 924
Mva1269I GAATGC 1 cut(s) 778
MvaI CCWGG 1 cut(s) 924
MwoI GCNNNNNNNGC 4 cut(s) 16, 273, 379, 784
NdeII GATC 5 cut(s) 27, 157, 199, 271, 742
NlaIII CATG 6 cut(s) 9, 164, 556, 650, 749, 824
NlaIV GGNNCC 2 cut(s) 29, 485
PagI TCATGA 3 cut(s) 160, 745, 820
PcsI WCGNNNNNNNCGW 3 cut(s) 81, 600, 851
PctI GAATGC 1 cut(s) 778
PfeI GAWTC 3 cut(s) 68, 641, 952
PflFI GACNNNGTC 3 cut(s) 173, 599, 850
PkrI GCNGC 6 cut(s) 12, 731, 734, 1023, 1071, 1087
PmaCI CACGTG 1 cut(s) 771
PmlI CACGTG 1 cut(s) 771
Ppu21I YACGTR 1 cut(s) 771
PshBI ATTAAT 1 cut(s) 108
Psp1406I AACGTT 1 cut(s) 797
Psp6I CCWGG 1 cut(s) 922
PspCI CACGTG 1 cut(s) 771
PspFI CCCAGC 1 cut(s) 248
PspGI CCWGG 1 cut(s) 922
PspN4I GGNNCC 2 cut(s) 29, 485
PspOMI GGGCCC 1 cut(s) 483
PspPI GGNCC 3 cut(s) 483, 484, 1104
PsuI RGATCY 1 cut(s) 27
PsyI GACNNNGTC 3 cut(s) 173, 599, 850
RsaI GTAC 2 cut(s) 524, 674
RsaNI GTAC 2 cut(s) 523, 673
SaqAI TTAA 6 cut(s) 108, 453, 464, 696, 971, 1080
SatI GCNGC 6 cut(s) 11, 730, 733, 1022, 1070, 1086
Sau3AI GATC 5 cut(s) 27, 157, 199, 271, 742
Sau96I GGNCC 3 cut(s) 483, 484, 1104
ScrFI CCNGG 1 cut(s) 924
SduI GDGCHC 1 cut(s) 487
SfaNI GCATC 2 cut(s) 292, 1081
SfcI CTRYAG 1 cut(s) 394
SinI GGWCC 1 cut(s) 1104
Sse9I AATT 6 cut(s) 105, 408, 664, 933, 977, 1097
SsiI CCGC 6 cut(s) 11, 188, 373, 577, 590, 1102
SspI AATATT 1 cut(s) 151
SspMI CTAG 1 cut(s) 783
StyD4I CCNGG 1 cut(s) 922
StyI CCWWGG 1 cut(s) 1107
TaaI ACNGT 2 cut(s) 238, 421
TaiI ACGT 9 cut(s) 177, 299, 534, 636, 678, 723, 773, 800, 851
TaqI TCGA 4 cut(s) 331, 384, 495, 560
TasI AATT 6 cut(s) 105, 408, 664, 933, 977, 1097
TauI GCSGC 1 cut(s) 13
TfiI GAWTC 3 cut(s) 68, 641, 952
Tru1I TTAA 6 cut(s) 108, 453, 464, 696, 971, 1080
Tru9I TTAA 6 cut(s) 108, 453, 464, 696, 971, 1080
TseI GCWGC 5 cut(s) 729, 732, 1021, 1069, 1085
Tth111I GACNNNGTC 3 cut(s) 173, 599, 850
VpaK11BI GGWCC 1 cut(s) 1104
VspI ATTAAT 1 cut(s) 108
XapI RAATTY 2 cut(s) 664, 977
XcmI CCANNNNNNNNNTGG 2 cut(s) 21, 466
XspI CTAG 1 cut(s) 783
ZraI GACGTC 3 cut(s) 175, 297, 849
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.