Rh3BG042800

Mediator-associated protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr3B
OG0014757
Physical Location & Seq
Forward (+)
2762567 .. 2763919
1353 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh3BG042800.1

Sequence Viewer

Length: 903 bp
ATGCCGTGGCCATTGCTAAAATGGATCATGTATACTTCTTCGTCGTCTTCCTCTTCCTCTGAAGATTTCAGTGACGCCGAAGATGAAGGCATAAGTAAGAAGGGCAATGATCATCGTCGTGATCCCAACAAACAAGGAGAACAAAATCACGATGACAACTCCGATGAAGATGACTTTTCCTACGAACCCCAGGAAGACGAGACCTCCAACTCCTCCGACTATACTTTCACAATCAACGAACCGCGCAGAAAAAGACGTCGCATTGACAACAAGGAAGCCTCAACAATTGTGAGTTTTAAACGCCGTAAGAGGATTTGGAGCAAGGAGGACGAGCTAGAGCTCTTGAAGAATTTCCTTGACTACAGAACCAGACCGAGGACGGCTAACGAAACCACGTCGTTTTACGACGAGGTTAACCCCAAGTTCAAGGACCAATTCAGCAAGCGAAAGCTGGCCGAGAAGCTTGCAAGGTTGAAGAAGAAGCACCGCAACGTAGTAAACAAAATGGTGAACAATAATGGGCAAGAGTTTCGCTTCAGAAACCCACACGACAGAGCCATTTTCGATATTTCACATAGGATTTGGGCAGAATCCCCTCTTTACAATGTTCAGGAGAAGAACGTTGTGGTGGATTCACATGATGTGGGGAGTAGCAAAGCGTCGTTCGATGAGAAGCATAACAATGGGGATGATGAGGGTAACACTGACGTGCGAGAAATGATAATCGGGTTGGCGATGAATCCGATACCGTTGAGTTTAAGTAACTATGGAGGCCGTGAAGGAGAAGTGGTGGATGAGAAGTGGAAGGAGCAGCAGATACTGGAGTTGGAGGCGTACTCGAAGAGGTTGGAGTTGGTTCAACATCAGGTTAAGGCAGCTTTGGATGACTTGCGTTCCAAGTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF573

Protein Analysis

300

Amino Acids

35.14

Weight (kDa)

5.86

Isoelectric Point (pI)

46.22

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
GeBP-like_DBD PF04504 103 - 195 1.1e-23 Glabrous-enhancer-binding protein-like family, DBD domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 259
AccI GTMKAC 1 cut(s) 32
AccII CGCG 1 cut(s) 244
AciI CCGC 2 cut(s) 242, 487
AclI AACGTT 1 cut(s) 621
AclWI GGATC 2 cut(s) 32, 116
AcoI YGGCCR 2 cut(s) 8, 453
AcsI RAATTY 1 cut(s) 349
AcuI CTGAAG 2 cut(s) 81, 520
AcyI GRCGYC 2 cut(s) 75, 256
AfaI GTAC 1 cut(s) 836
AfiI CCNNNNNNNGG 1 cut(s) 375
AgsI TTSAA 4 cut(s) 346, 427, 475, 860
AjiI CACGTC 2 cut(s) 396, 709
AjnI CCWGG 1 cut(s) 189
AleI CACNNNNGTG 1 cut(s) 707
AluBI AGCT 5 cut(s) 334, 340, 451, 463, 878
AluI AGCT 5 cut(s) 334, 340, 451, 463, 878
Alw21I GWGCWC 1 cut(s) 342
Alw26I GTCTC 1 cut(s) 194
AlwI GGATC 2 cut(s) 32, 116
AlwNI CAGNNNCTG 1 cut(s) 820
AoxI GGCC 3 cut(s) 8, 453, 772
ApeKI GCWGC 2 cut(s) 811, 875
ApoI RAATTY 1 cut(s) 349
Asp700I GAANNNNTTC 1 cut(s) 350
AspLEI GCGC 1 cut(s) 246
AspS9I GGNCC 1 cut(s) 430
AsuHPI GGTGA 1 cut(s) 520
AvaII GGWCC 1 cut(s) 430
BaeI ACNNNNGTAYC 2 cut(s) 809, 842
BalI TGGCCA 1 cut(s) 10
BanII GRGCYC 1 cut(s) 342
BbsI GAAGAC 2 cut(s) 39, 201
Bbv12I GWGCWC 1 cut(s) 342
BbvI GCAGC 2 cut(s) 823, 887
BceAI ACGGC 3 cut(s) 288, 396, 759
BcgI CGANNNNNNTGC 4 cut(s) 446, 480, 512, 546
BciT130I CCWGG 1 cut(s) 191
BclI TGATCA 1 cut(s) 109
BcoDI GTCTC 1 cut(s) 194
BfaI CTAG 1 cut(s) 335
BfmI CTRYAG 1 cut(s) 361
BisI GCNGC 2 cut(s) 812, 876
BlsI GCNGC 2 cut(s) 813, 877
Bme1390I CCNGG 1 cut(s) 191
Bme18I GGWCC 1 cut(s) 430
BmgBI CACGTC 2 cut(s) 396, 709
BmgT120I GGNCC 1 cut(s) 430
BmrFI CCNGG 1 cut(s) 191
BpiI GAAGAC 2 cut(s) 39, 201
BplI GAGNNNNNCTC 2 cut(s) 821, 853
BpmI CTGGAG 1 cut(s) 842
BsaHI GRCGYC 2 cut(s) 75, 256
BsaI GGTCTC 1 cut(s) 194
BsaJI CCNNGG 3 cut(s) 5, 189, 374
BsaXI ACNNNNNCTCC 4 cut(s) 188, 218, 774, 804
Bsc4I CCNNNNNNNGG 1 cut(s) 375
Bse1I ACTGG 1 cut(s) 825
Bse3DI GCAATG 2 cut(s) 11, 112
BseBI CCWGG 1 cut(s) 191
BseDI CCNNGG 3 cut(s) 5, 189, 374
BseGI GGATG 3 cut(s) 694, 799, 889
BseLI CCNNNNNNNGG 1 cut(s) 375
BseMI GCAATG 2 cut(s) 11, 112
BseNI ACTGG 1 cut(s) 825
BseRI GAGGAG 1 cut(s) 202
BseXI GCAGC 2 cut(s) 823, 887
Bsh1236I CGCG 1 cut(s) 244
BshFI GGCC 3 cut(s) 10, 455, 774
BsiHKAI GWGCWC 1 cut(s) 342
BslI CCNNNNNNNGG 1 cut(s) 375
BsmAI GTCTC 1 cut(s) 194
BsnI GGCC 3 cut(s) 10, 455, 774
Bso31I GGTCTC 1 cut(s) 194
Bsp1286I GDGCHC 1 cut(s) 342
Bsp143I GATC 3 cut(s) 24, 109, 121
BspACI CCGC 2 cut(s) 242, 487
BspANI GGCC 3 cut(s) 10, 455, 774
BspFNI CGCG 1 cut(s) 244
BspPI GGATC 2 cut(s) 32, 116
BspTNI GGTCTC 1 cut(s) 194
BsrDI GCAATG 2 cut(s) 11, 112
BsrI ACTGG 1 cut(s) 825
BssECI CCNNGG 3 cut(s) 5, 189, 374
BssMI GATC 3 cut(s) 24, 109, 121
BssNAI GTATAC 1 cut(s) 33
BssNI GRCGYC 2 cut(s) 75, 256
Bst1107I GTATAC 1 cut(s) 33
Bst2UI CCWGG 1 cut(s) 191
Bst4CI ACNGT 1 cut(s) 750
Bst6I CTCTTC 2 cut(s) 58, 836
BstACI GRCGYC 2 cut(s) 75, 256
BstC8I GCNNGC 3 cut(s) 443, 453, 465
BstDSI CCRYGG 1 cut(s) 5
BstF5I GGATG 3 cut(s) 694, 799, 889
BstFNI CGCG 1 cut(s) 244
BstHHI GCGC 1 cut(s) 246
BstKTI GATC 3 cut(s) 27, 112, 124
BstMAI GTCTC 1 cut(s) 194
BstMBI GATC 3 cut(s) 24, 109, 121
BstNI CCWGG 1 cut(s) 191
BstSCI CCNGG 1 cut(s) 189
BstSFI CTRYAG 1 cut(s) 361
BstUI CGCG 1 cut(s) 244
BstV1I GCAGC 2 cut(s) 823, 887
BstV2I GAAGAC 2 cut(s) 39, 201
BstZ17I GTATAC 1 cut(s) 33
BsuRI GGCC 3 cut(s) 10, 455, 774
BtgI CCRYGG 1 cut(s) 5
BtgZI GCGATG 1 cut(s) 749
BtrI CACGTC 2 cut(s) 396, 709
BtsCI GGATG 3 cut(s) 694, 799, 889
BtsIMutI CAGTG 2 cut(s) 76, 702
Cac8I GCNNGC 3 cut(s) 443, 453, 465
CaiI CAGNNNCTG 1 cut(s) 820
CfoI GCGC 1 cut(s) 246
Cfr13I GGNCC 1 cut(s) 430
CseI GACGC 2 cut(s) 83, 648
Csp6I GTAC 1 cut(s) 835
CviAII CATG 2 cut(s) 28, 638
CviQI GTAC 1 cut(s) 835
DpnI GATC 3 cut(s) 26, 111, 123
DpnII GATC 3 cut(s) 24, 109, 121
DraI TTTAAA 1 cut(s) 298
EaeI YGGCCR 2 cut(s) 8, 453
Eam1104I CTCTTC 2 cut(s) 58, 836
EarI CTCTTC 2 cut(s) 58, 836
Ecl136II GAGCTC 1 cut(s) 340
Eco24I GRGCYC 1 cut(s) 342
Eco31I GGTCTC 1 cut(s) 194
Eco47I GGWCC 1 cut(s) 430
Eco53kI GAGCTC 1 cut(s) 340
Eco57I CTGAAG 2 cut(s) 81, 520
EcoICRI GAGCTC 1 cut(s) 340
EcoRII CCWGG 1 cut(s) 189
EcoT38I GRGCYC 1 cut(s) 342
FaeI CATG 2 cut(s) 31, 641
FaiI YATR 8 cut(s) 29, 33, 92, 222, 576, 639, 678, 768
FatI CATG 2 cut(s) 27, 637
FbaI TGATCA 1 cut(s) 109
FblI GTMKAC 1 cut(s) 32
Fnu4HI GCNGC 2 cut(s) 812, 876
FokI GGATG 3 cut(s) 701, 806, 896
FriOI GRGCYC 1 cut(s) 342
Fsp4HI GCNGC 2 cut(s) 812, 876
FspBI CTAG 1 cut(s) 335
GlaI GCGC 1 cut(s) 245
GluI GCNGC 2 cut(s) 812, 876
GsuI CTGGAG 1 cut(s) 842
HaeIII GGCC 3 cut(s) 10, 455, 774
HgaI GACGC 2 cut(s) 83, 648
HhaI GCGC 1 cut(s) 246
Hin1I GRCGYC 2 cut(s) 75, 256
Hin1II CATG 2 cut(s) 31, 641
Hin6I GCGC 1 cut(s) 244
HinP1I GCGC 1 cut(s) 244
HincII GTYRAC 1 cut(s) 415
HindII GTYRAC 1 cut(s) 415
HindIII AAGCTT 1 cut(s) 461
HinfI GANTC 3 cut(s) 590, 632, 739
HpaI GTTAAC 1 cut(s) 415
HphI GGTGA 1 cut(s) 520
Hpy166II GTNNAC 4 cut(s) 33, 415, 499, 511
Hpy188I TCNGA 5 cut(s) 61, 163, 217, 539, 744
Hpy188III TCNNGA 4 cut(s) 119, 149, 343, 611
Hpy8I GTNNAC 4 cut(s) 33, 415, 499, 511
Hpy99I CGWCG 6 cut(s) 46, 120, 261, 400, 410, 664
HpyAV CCTTC 4 cut(s) 80, 94, 773, 799
HpyCH4III ACNGT 1 cut(s) 750
HpyCH4IV ACGT 5 cut(s) 256, 395, 492, 621, 708
HpyCH4V TGCA 1 cut(s) 467
HpySE526I ACGT 5 cut(s) 256, 395, 492, 621, 708
Hsp92I GRCGYC 2 cut(s) 75, 256
Hsp92II CATG 2 cut(s) 31, 641
HspAI GCGC 1 cut(s) 244
Ksp22I TGATCA 1 cut(s) 109
KspAI GTTAAC 1 cut(s) 415
Kzo9I GATC 3 cut(s) 24, 109, 121
LmnI GCTCC 2 cut(s) 318, 808
LpnPI CCDG 7 cut(s) 176, 203, 382, 437, 596, 806, 851
Lsp1109I GCAGC 2 cut(s) 823, 887
MaeI CTAG 1 cut(s) 335
MaeII ACGT 5 cut(s) 256, 395, 492, 621, 708
MaeIII GTNAC 3 cut(s) 71, 698, 761
MalI GATC 3 cut(s) 26, 111, 123
MboI GATC 3 cut(s) 24, 109, 121
MfeI CAATTG 1 cut(s) 285
MhlI GDGCHC 1 cut(s) 342
MlsI TGGCCA 1 cut(s) 10
MluCI AATT 3 cut(s) 285, 349, 434
MluNI TGGCCA 1 cut(s) 10
MmeI TCCRAC 4 cut(s) 231, 240, 807, 828
Mox20I TGGCCA 1 cut(s) 10
MroXI GAANNNNTTC 1 cut(s) 350
MscI TGGCCA 1 cut(s) 10
MseI TTAA 4 cut(s) 297, 414, 758, 870
MslI CAYNNNNRTG 3 cut(s) 117, 681, 707
Msp20I TGGCCA 1 cut(s) 10
MspR9I CCNGG 1 cut(s) 191
MunI CAATTG 1 cut(s) 285
MvaI CCWGG 1 cut(s) 191
MvnI CGCG 1 cut(s) 244
NdeII GATC 3 cut(s) 24, 109, 121
NlaIII CATG 2 cut(s) 31, 641
NmeAIII GCCGAG 1 cut(s) 481
NmuCI GTSAC 1 cut(s) 71
OliI CACNNNNGTG 1 cut(s) 707
PdmI GAANNNNTTC 1 cut(s) 350
PfeI GAWTC 3 cut(s) 590, 632, 739
PkrI GCNGC 2 cut(s) 813, 877
Psp124BI GAGCTC 1 cut(s) 342
Psp1406I AACGTT 1 cut(s) 621
Psp6I CCWGG 1 cut(s) 189
PspGI CCWGG 1 cut(s) 189
PspPI GGNCC 1 cut(s) 430
PstNI CAGNNNCTG 1 cut(s) 820
RsaI GTAC 1 cut(s) 836
RsaNI GTAC 1 cut(s) 835
RseI CAYNNNNRTG 3 cut(s) 117, 681, 707
SacI GAGCTC 1 cut(s) 342
SaqAI TTAA 4 cut(s) 297, 414, 758, 870
SatI GCNGC 2 cut(s) 812, 876
Sau3AI GATC 3 cut(s) 24, 109, 121
Sau96I GGNCC 1 cut(s) 430
ScrFI CCNGG 1 cut(s) 191
SduI GDGCHC 1 cut(s) 342
SfcI CTRYAG 1 cut(s) 361
SinI GGWCC 1 cut(s) 430
SmiMI CAYNNNNRTG 3 cut(s) 117, 681, 707
Sse9I AATT 3 cut(s) 285, 349, 434
SsiI CCGC 2 cut(s) 242, 487
SspMI CTAG 1 cut(s) 335
SstI GAGCTC 1 cut(s) 342
StyD4I CCNGG 1 cut(s) 189
TaaI ACNGT 1 cut(s) 750
TaiI ACGT 5 cut(s) 259, 398, 495, 624, 711
TaqI TCGA 3 cut(s) 564, 666, 839
TaqII GACCGA 1 cut(s) 388
TasI AATT 3 cut(s) 285, 349, 434
TfiI GAWTC 3 cut(s) 590, 632, 739
Tru1I TTAA 4 cut(s) 297, 414, 758, 870
Tru9I TTAA 4 cut(s) 297, 414, 758, 870
TscAI CASTG 2 cut(s) 76, 709
TseFI GTSAC 1 cut(s) 71
TseI GCWGC 2 cut(s) 811, 875
Tsp45I GTSAC 1 cut(s) 71
TspDTI ATGAA 3 cut(s) 99, 180, 752
TspRI CASTG 2 cut(s) 76, 709
VpaK11BI GGWCC 1 cut(s) 430
XapI RAATTY 1 cut(s) 349
XcmI CCANNNNNNNNNTGG 1 cut(s) 18
XmiI GTMKAC 1 cut(s) 32
XmnI GAANNNNTTC 1 cut(s) 350
XspI CTAG 1 cut(s) 335
ZraI GACGTC 1 cut(s) 257
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.